CN113584180B - Application of exosome miRNA derived from endometrium in embryo implantation - Google Patents
Application of exosome miRNA derived from endometrium in embryo implantation Download PDFInfo
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- CN113584180B CN113584180B CN202110761587.8A CN202110761587A CN113584180B CN 113584180 B CN113584180 B CN 113584180B CN 202110761587 A CN202110761587 A CN 202110761587A CN 113584180 B CN113584180 B CN 113584180B
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- 108091070501 miRNA Proteins 0.000 title abstract description 38
- 239000002679 microRNA Substances 0.000 title abstract description 36
- 230000032692 embryo implantation Effects 0.000 title abstract description 16
- 210000004696 endometrium Anatomy 0.000 title abstract description 12
- 210000001808 exosome Anatomy 0.000 title abstract description 4
- 241000699666 Mus <mouse, genus> Species 0.000 claims abstract description 145
- 241000699670 Mus sp. Species 0.000 claims abstract description 100
- 230000009471 action Effects 0.000 claims abstract description 16
- 230000000903 blocking effect Effects 0.000 claims description 23
- 230000000694 effects Effects 0.000 abstract description 15
- 238000002513 implantation Methods 0.000 abstract description 13
- 230000008859 change Effects 0.000 abstract description 11
- 230000003111 delayed effect Effects 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 6
- 230000004913 activation Effects 0.000 abstract description 5
- 230000035935 pregnancy Effects 0.000 abstract description 5
- 238000012163 sequencing technique Methods 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 4
- 230000002357 endometrial effect Effects 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 4
- 108091032955 Bacterial small RNA Proteins 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 16
- 210000001519 tissue Anatomy 0.000 description 10
- 210000003739 neck Anatomy 0.000 description 7
- 108091066337 Mus musculus miR-192 stem-loop Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 238000012167 Small RNA sequencing Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000012173 estrus Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 108091086416 miR-192 stem-loop Proteins 0.000 description 2
- 239000003128 rodenticide Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 101100460719 Mus musculus Noto gene Proteins 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- 239000003433 contraceptive agent Substances 0.000 description 1
- 230000002254 contraceptive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002282 effect on embryo Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001119 rodenticidal effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses an application of endometrium-derived exosome miRNA in embryo implantation, which comprises the following steps: step A, sequencing small RNA: the pregnant mice are fixed by the mice fixing device 3, and then the mice are sacrificed; step B, analyzing the change of gestation miRNA of the pregnant mice; step C, verifying the action function of miRNA by a pseudo-pregnancy model; step D, verifying the action function of miRNA by a delayed implantation model and a delayed implantation activation model; step E, exploring the effect of miRNA as a drug for inhibiting embryo implantation in a mouse body; the automatic fixing device for the mice in the step A comprises a fixing device; the fixing device comprises a mouse foot leveling component; through having set up the mouse foot and having pulled flat subassembly, fix the four limbs of mice to pulling four limbs all around, thereby make the mice be in the state of lying prone down, thereby make the mice unable to break loose and thereby improve the accuracy to exosome miRNA effect test of endometrial source.
Description
Technical Field
The invention belongs to the technical field of miRNA application, and particularly relates to application of exocrine miRNA derived from endometrium in embryo implantation.
Background
miRNA has higher expression level in spleen, kidney and liver in the organism. Recently, research shows that the consistency of human miRNA and mouse miRNA reaches 100%, so that mice can be selected as model animals, the influence of miRNA on embryo implantation is researched, and medicines for inhibiting or promoting embryo implantation are developed; however, in the existing experiments, the mice need to be fixed, then corresponding experimental objects are extracted from the bodies of the mice, but the mice need to be fixed by hands in the extraction process, because the sizes of hands of people are different, each experimenter cannot be guaranteed to fix the mice well, so that the stability of sampling the mice can be affected, the mice themselves are difficult to fix, the effect of fixing the mice is poor, and the phenomenon of inaccurate sampling data can be caused in the sampling process.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides an application of exocrine miRNA from endometrium in embryo implantation, which can better fix mice in the process of exocrine miRNA test from endometrium and improve the test stability and accuracy of exocrine miRNA effect.
In order to achieve the above purpose, the present invention adopts the following technical scheme: use of exocrine mirnas derived from endometrium in embryo implantation, comprising the steps of:
step A, sequencing small RNA: selecting a female mouse and a male mouse for estrus, carrying out cage combination, conception on the female mouse, fixing a pregnant white mouse through automatic white mouse fixing equipment, then killing the white mouse, and extracting tissue miRNA; finding miRNA playing a key role in the implantation window period from the sequencing result, and mainly observing the change of the mmu-miR-192-5p before and after implantation in the embryo implantation process;
step B, analyzing the change of gestation miRNA of the pregnant mice; the change trend of miR-192-5p on the first day to the seventh day is mainly recorded and analyzed.
Step C, verifying the action function of miRNA by a pseudo-pregnancy model; in the pseudopregnant model, in the absence of embryos, a change in the appearance of mmu-miR-192-5p in the endometrium compared to the first day of gestation;
step D, verifying the action function of miRNA by a delayed implantation model and a delayed implantation activation model; analysis of data results of the time delay model and the time delay activation model mainly proves that implantation events are the reasons for the change of the mmu-miR-192-5p during embryo implantation
And E, exploring the effect of miRNA as a drug for inhibiting embryo implantation in a mouse body. .
The automatic mouse fixing device in the step A comprises a first supporting frame, a mouse cage, a cage door, a first baffle, a door device and a fixing device, wherein the mouse cage is arranged above the first supporting frame and used for closing a mouse, the cage door is arranged on the mouse cage and used for controlling the mouse to go in and out, the first baffle is arranged on the side face of the mouse cage and used for controlling the mouse to leave the mouse cage and not deviate, the door device is arranged at the end part of the first baffle and used for controlling the mouse to drop down, and the fixing device is arranged below the door device and used for fixing the mouse; the fixing device comprises a first box body fixedly arranged below the first supporting frame and used for containing mice, a lifting plate arranged in the first box body and used for enabling the mice to move up and down, a cylinder arranged below the first box body and used for driving the lifting plate, a mouse foot leveling component arranged on the lifting plate and used for fixing limbs of the mice, two first control components arranged on two sides of the first box body and used for being matched with the lifting plate to drive the mouse foot leveling component, two second control components arranged on two sides of the first box body and positioned above the first control components and used for controlling the first control components not to drive the mouse foot leveling component, two first fixing plates arranged above the lifting plate and positioned on two sides of the first box body and used for pressing the mice, a third control component arranged on two sides of the first box body and connected with the two first fixing plates and used for being matched with the first control components to control the first fixing plates to move towards the middle position of the first box body, and a fourth control component arranged on two sides of the first box body and positioned above the third control components and used for controlling the first fixing plates to move up and down, and a mouse killing component arranged on the neck of the mice; the mouse foot leveling assembly comprises two first guide rods connected to the first control assembly, two first guide blocks arranged on the first guide rods in a sliding manner, a swinging structure arranged on the first guide blocks and connected to the lifting plate for controlling the first guide blocks to slide on the first guide rods, two third baffle plates arranged on the lifting plate and used for clamping mice, a second avoidance groove arranged on the third baffle plates and used for avoiding positions with the swinging structure, an automatic clamping structure arranged on the first guide blocks and used for clamping limbs of the mice, and a limit rod arranged on the lifting plate and used for being matched with the swinging structure; when the device is used, a mouse comes out of a mouse cage, reaches a door blocking device along a first baffle, falls onto a lifting plate, is clamped by a first control component, enables the mouse to rotate in a direction, then drives the lifting plate to move upwards by a cylinder, and drives a first guide rod to move towards two sides of a first box body by matching with the first control component when the lifting plate moves upwards, then drives a first guide block to move together, then drives the first guide block to move towards two sides of the first guide rod under the action of a swinging structure, and then drives the automatic clamping structure to move, the automatic clamping structure clamps four limbs of the mouse and stretches towards the periphery of the mouse, so that the mouse is prone to be on the lifting plate, the automatic clamping structure is matched with a limit rod when moving, the automatic clamping structure clamps four limbs of the mouse to stretch towards the periphery, and then the first control component is matched with a second control component, so that the first guide rod and the first guide block do not move towards the two sides of the first guide block, and then the first control component is fixed and moves upwards, and the first control component is fixed and moves upwards; the mouse can fall on the lifter plate automatically, the convenience of use has been increased, afterwards, first guide bar can drive lifter plate upward movement, can make the four limbs of mouse fixed when lifter plate upward movement, and pull around, can make the better quilt of mouse fixed on the lifter plate, have the mouse can lie prone on the lifter plate in view of, so the mouse also can not use four limbs to struggle, thereby the fixed effect of mouse has further been increased, first fixed plate can press on the mouse before fixing the mouse four limbs, thereby make the clamp structure clamp the four limbs of mouse that can be better, and stretch around, the success rate to mouse four limbs is increased, make the mouse can't break loose when being fixed, the stability when having increased this equipment is used, the cooperation of first control module and second control module, can make the mouse by the automatic clamp structure just stop stretching after fixed, avoid the mouse to receive big tensile force to lead to the bad, thereby the mouse can be fixed by the healthy experiment of mouse, thereby the position can be better fixed to the mouse, the experiment can be more stable position can be opened to the help the fixed position, thereby the experiment can be better, the experiment can be carried out to the position is better, the experiment can be opened, the experiment can be carried out, the experiment can be better, the position is better, the experiment can be carried out, the experiment can be well, the position is better, the position can be kept up to the experiment can be the top has been well, and can be fixed, and can be had, the stability is better, the experiment can be the stability is well is used.
Further, the tissue miRNA extraction in the step A is performed by using a tissue grinder on endometrial tissues, and then analyzing the result of small RNA sequencing.
Furthermore, the tissue grinding instrument uses an instrument with better high-flux grinding effect.
Further, the automatic clamping structure comprises a fixed sleeve fixedly arranged on the automatic clamping structure, a fifth rotating shaft rotationally arranged in the fixed sleeve, a third supporting frame fixedly arranged on the fifth rotating shaft and used for pushing the limbs of the mice, a second clamping plate arranged on the third supporting frame and used for clamping the limbs of the mice, a sliding rod slidingly arranged on the third supporting frame, a fourth rotating shaft rotationally arranged on the sliding rod, a first clamping plate fixedly arranged on the fourth rotating shaft and used for clamping the limbs of the mice, a rotating part arranged above the fourth rotating shaft and used for controlling the first clamping plate to rotate, a rope connected with the rotating part and the fixed sleeve, and a guiding part arranged on the third supporting frame and used for guiding the rope; the second clamping plate is provided with a plurality of first bulges; a plurality of second bulges are arranged on the first clamping plate; after the mice are held by first fixed plate, the third support frame can strike the four limbs of mice, at this moment the mice can be owing to by being pushed down, the four limbs are used for supporting the health and can not fall, thereby unable lift, thereby the third support frame can be better push away the four limbs of mice and make it can't avoid the third support frame, afterwards, the length of mice four limbs is limited, and the one end of four limbs is fixed on the mice health, so can make the four limbs of mice incline when pushing the mice all around, afterwards, the third support frame can incline along with the joint slope, thereby also be rotatory in the fifth pivot, can lead to the rope to be pulled behind the rotatory of third support frame, thereby can make first grip block rotate under the effect of rotating part, the first grip block can rotate the back and hold the four limbs of mice, thereby can make the four limbs of mice to be pressed from both sides tightly, the centre gripping, afterwards, because the mice are used for supporting on the body of mice, so can be greatly when pushing the mouse, can make the third support frame, thereby can be very good to make the mouse, the four limbs can be pressed from both sides tightly by the device to the second, the four limbs can be held by the fixed by the mouse, thereby the four limbs can be held by the device is kept up by the four limbs to the fixed to the mouse, the four limbs can be kept up by the fixed by the mouse, the device is kept up because of the four limbs can be held by the fixed to the four limbs of the mouse, the four limbs can be because of the mouse is very good, the four limbs can be held by the fixed, the four limbs can be because of the device can be kept down by the four limbs.
Further, the automatic clamping structure further comprises a second supporting plate fixedly arranged on the sliding rod and a fifth spring arranged between the second supporting plate and the third supporting frame; the rotating part comprises a third gear fixedly arranged on the fourth rotating shaft, a second rack meshed with the third gear and fixed on the third supporting frame, and a second fixing block rotatably arranged above the fourth rotating shaft and fixedly connected with the rope; the guide part comprises a first fixed block fixedly arranged on the third support frame, a sixth rotating shaft rotatably arranged on the first fixed block and a guide wheel fixedly arranged on the sixth rotating shaft; the number of teeth on the second rack is only half of the number of teeth on the third gear; the rope is wound on the guide wheel; the rope pulls the second fixed block to move to the sliding rod can slide on the third support frame, and the number of teeth on the second rack is only half of the number of teeth on the third gear can make the third gear drive the fourth pivot to rotate one quarter circle, makes first grip block and second grip block keep parallel, can make first grip block be close to the second grip block when the rope pulls the second fixed block, thereby can make the four limbs of mice can be fixed tighter, later utilize the fifth spring to make first grip block reset, and need not the power supply just can realize the fixed of four limbs of mice.
Further, the swing structure comprises a fifth connecting rod shutter device fixed above the first guide block, a third connecting rod hinged to the first guide block, a fourth connecting rod fixed to the third connecting rod, a fifth connecting rod hinged to the fourth connecting rod, a first control plate fixedly arranged on the fourth connecting rod and contacted with the fifth connecting rod, a second support frame fixedly arranged on the fourth connecting rod, a ninth guide rod slidingly arranged in the second support frame, a second control plate fixedly arranged on the ninth guide rod and propped against the fifth connecting rod, and a fourth spring arranged between the second support frame and the second control plate; the limit rod piece is arranged on the side face of the fifth connecting rod; when the first guide rod moves to two sides, the third connecting rod, the fourth connecting rod and the fifth connecting rod enable the first guide block to move to two sides of the first guide rod, then the fifth connecting rod impacts the limit rod to enable the third connecting rod and the fourth connecting rod to rotate around the hinge point of the fourth connecting rod and the hinge point of the fifth connecting rod, so that the range that the first guide block moves to two sides on the first guide rod is increased, the first guide block can be enabled to move on the first guide rod better, and the effect of fixing the limbs of a mice by the automatic clamping structure and the stability of fixing are increased.
Further, the first control component comprises a plurality of first sliding grooves formed in two side surfaces of the first box body, a plurality of second sliding grooves formed in the lifting plate, a first guide plate arranged on the first sliding grooves in a sliding mode, a first supporting plate arranged on the first guide plate in the second sliding grooves in a sliding mode, a fifth sliding groove and a sixth sliding groove formed in the first guide plate, a third limiting block arranged on the first supporting plate and used for being clamped on the second sliding grooves, a first rod piece fixedly arranged between the two first guide plates, a first connecting rod hinged to the first rod piece, a second guide rod fixedly arranged on two sides of the first box body, a second guide plate arranged on the second guide rod in a sliding mode and arranged in the sixth sliding groove in a sliding mode, and a third guide rod fixedly arranged on the second guide plate and used for controlling the movement of the first guide plate; the second control assembly comprises a second fixed plate fixedly arranged on the side surface of the first box body and distributed up and down, a second guide block slidably arranged on the fourth guide rod, a first spring arranged between the second fixed plate and the second guide block, and a second rod piece arranged on the second guide block and hinged to the first connecting rod; the lifter plate upward movement can make first deflector to both sides move, third stopper and first backup pad can be that first deflector is better carries out the slip on the lifter plate, afterwards, first member is kept away the effect of position groove at the second and is moved to both sides, realize basic motion, drive the mouse foot and draw down the subassembly and carry out the centre gripping to the mouse fixedly, but the mode of centre gripping is to stretching the four limbs of mouse all around, so can not draw always, so the lifter plate is continuous upwards movement but first deflector can not continue to move to both sides after stretching to a certain extent, so can take place the third guide bar and strike the second guide block, make second guide bar and second member keep away the position groove along with first fixed plate is first and upward movement together, thereby two pin joints and first connecting rod of first connecting rod all can upward synchronous motion, so first deflector can not move to both sides, first guide plate only can upward movement, the sliding connection of sixth spout and second guide plate can be better make first control assembly and second control assembly realize relevant cooperation, the stability of mouse when having realized the operation is better, the stability of mouse is fixed.
Further, the third control assembly comprises a fifth guide rod fixedly arranged below the first support frame, a third guide plate slidingly arranged on the fifth guide rod, a third guide block fixedly arranged on the third guide plate and slidingly connected with a third limiting block, a second connecting rod hinged to the third guide block, a fourth guide block hinged to the other end of the third guide block, a sixth guide rod fixedly arranged below the first support frame and slidingly connected with the fourth guide block for enabling the fourth guide block to move up and down, a seventh guide rod fixedly arranged on the sixth guide rod and the first fixing plate, a fifth guide block fixedly arranged on the seventh guide rod, a second spring arranged between the fifth guide block and the first box and used for enabling the first fixing plate to reset, and a moving groove arranged on the first box and used for enabling the seventh guide rod to move up and down; the fourth control assembly comprises a third spring, a fifth guide plate, a third rod piece, a sixth guide plate, a third chute, a second limiting block, a sixth guide plate and a seventh guide plate, wherein the third spring is arranged between the fourth guide block and the first support frame and is used for enabling the fourth guide block to be incapable of moving upwards at the first time; the third guide plate and the fifth guide rod are used for enabling the third guide block to only move up and down; the sliding connection of the third guide block and the third limiting block and the sliding connection of the third guide plate and the fifth guide rod enable the third guide block to move upwards along with the first guide plate instead of moving to two sides along with the first guide plate, then the third spring enables the fourth guide block to drive the fifth guide plate to bear downward pressure, and accordingly the fourth guide block is pressed on the sixth guide plate, large force is needed for the upward movement of the fourth guide block, the fact that the first guide plate moves to two sides to drive the sixth guide block to slide in the third sliding groove instead of moving upwards is achieved, then the first guide plate and the second rod are impacted together when moving upwards, continuous movement cannot be continued, at the moment, the seventh guide rod is enabled to move upwards, and therefore the first fixing plate is driven to move upwards, the first fixing plate can be pressed on the back of a mouse instead of pressing the mouse, and the mouse is enabled to be fixed better.
Further, the mouse killing component comprises an eighth guide rod fixedly arranged below the first box body, a fourth guide plate slidingly arranged on the eighth guide rod, a first limiting block arranged below the eighth guide rod and used for clamping the fourth guide plate, a threaded sleeve rotationally arranged on the fourth guide plate, a threaded rod fixedly arranged in the threaded sleeve in a threaded connection manner, a mouse killing plate fixedly connected to the threaded rod and used for clamping the neck of a mouse, and bearings fixedly arranged on the threaded sleeve and positioned at the upper position and the lower position of the fourth guide plate and used for clamping the threaded sleeve; through the rotation of screw sleeve, control the reciprocates of the board that kills, make the board that kills can support on the neck of mouse when needing to kill the mouse, just descend the position that can not contact the mouse when need not killing the mouse, increased the effect that the device used.
Further, the blocking devices comprise a first rotating shaft, a second blocking plate, a first position avoiding groove, a first connecting piece, a first rack, a first gear, a second rotating shaft, a second gear, a first groove, a torsion spring and a fourth sliding groove, wherein the first rotating shaft is rotatably arranged in the blocking device, the second blocking plate is fixedly arranged on the first rotating shaft and used for blocking the first box body, the first position avoiding groove is arranged in the blocking device and used for avoiding the second blocking plate, the first connecting piece is fixedly arranged on the lifting plate, the first rack is fixedly arranged on the first connecting piece, the first gear is arranged on the first rotating shaft, the second rotating shaft is rotatably arranged at the first position avoiding groove, the second gear is fixedly arranged on the second rotating shaft and used for being meshed with the first rack to control the first gear to rotate, the first groove is formed in the second blocking plate, the torsion spring is arranged in the first groove and the fourth sliding groove is arranged on the first box body and used for enabling the first rack to slide up and down; when the mice arrive above the second baffle, the first rack and the second gear are not meshed, so the second baffle can rotate, so that the mice fall into the lifting plate at the second baffle, then the second baffle can rotate for half a circle through the first rack when the lifting plate moves upwards, so that the lifting plate is exposed outside, the mice at the fixed position are lifted, the equipment can be used for fixing the mice better, the convenience of experiments is improved, and the accuracy of the experiments can be better ensured.
In summary, the four limbs of the mice are fixed by arranging the mouse foot leveling component, and the four limbs are pulled to the periphery, so that the mice are in a lying state, the mice cannot break loose, the mice can be better fixed, the stability and the accuracy of a sampling process are not affected by the struggling of the mice when the mice are sampled later, the application of exocrine miRNA from endometrium is better obtained through experiments on the mice, the accuracy of the effect test on the exocrine miRNA from endometrium is improved, and the effect of the exocrine miRNA from endometrium is better found; experiments prove that miRNA has extremely remarkable inhibition effect on embryo implantation, and the miRNA can be used as a contraceptive medicament, so that a new way is provided for screening new medicaments.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of a door stop device according to the present invention;
FIG. 3 is a schematic view of a first groove structure according to the present invention;
FIG. 4 is a schematic view of a third baffle plate according to the present invention;
FIG. 5 is a schematic view of the structure of a first rack in the present invention;
FIG. 6 is a schematic view of a fourth chute according to the present invention;
FIG. 7 is a schematic cross-sectional view of a fastening device according to the present invention;
FIG. 8 is a schematic view showing the structure of the first case outside according to the present invention;
FIG. 9 is a schematic view of a first guide plate according to the present invention;
FIG. 10 is a schematic diagram of a second control assembly according to the present invention;
FIG. 11 is a schematic diagram of a fourth control assembly according to the present invention;
FIG. 12 is a schematic view of a third rod member according to the present invention;
FIG. 13 is a schematic view of a mouse foot leveling assembly according to the present invention;
FIG. 14 is an enlarged view of A of FIG. 13 in accordance with the present invention;
FIG. 15 is an enlarged view of B of FIG. 13 in accordance with the present invention;
FIG. 16 is a schematic view of a swing structure according to the present invention;
FIG. 17 is a schematic structural view of a second support frame according to the present invention;
FIG. 18 is a schematic view of the structure of the automatic clamping structure of the present invention;
FIG. 19 is an enlarged view of C of FIG. 18 in accordance with the present invention;
FIG. 20 is an enlarged view of D of FIG. 18 in accordance with the present invention;
FIG. 21 is a schematic view of a fifth shaft according to the present invention;
FIG. 22 is a schematic diagram of a rodenticidal assembly according to the present invention;
FIG. 23 is a schematic view of the structure of the first guide block according to the present invention;
Detailed Description
In order to enable those skilled in the art to better understand the present invention, the following description will make clear and complete descriptions of the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention.
As shown in fig. 1-23, the use of exocrine mirnas of endometrial origin in embryo implantation comprises the steps of:
step A, sequencing small RNA: selecting a female mouse and a male mouse for estrus, carrying out cage combination, conception on the female mouse, fixing a pregnant white mouse through automatic white mouse fixing equipment, then killing the white mouse, and extracting tissue miRNA; finding miRNA playing a key role in the implantation window period from the sequencing result, and mainly observing the change of the mmu-miR-192-5p before and after implantation in the embryo implantation process;
step B, analyzing the change of gestation miRNA of the pregnant mice; the change trend of miR-192-5p on the first day to the seventh day is mainly recorded and analyzed.
Step C, verifying the action function of miRNA by a pseudo-pregnancy model; in the pseudopregnant model, in the absence of embryos, a change in the appearance of mmu-miR-192-5p in the endometrium compared to the first day of gestation;
step D, verifying the action function of miRNA by a delayed implantation model and a delayed implantation activation model; analysis of data results of the time delay model and the time delay activation model mainly proves that implantation events are the reasons for the change of the mmu-miR-192-5p during embryo implantation
And E, exploring the effect of miRNA as a drug for inhibiting embryo implantation in a mouse body.
The automatic mouse fixing device in the step A comprises a first support frame 1, a mouse cage 2 arranged above the first support frame 1 and used for closing a mouse, a cage door arranged on the mouse cage 2 and used for controlling the mouse to come in and go out, a first baffle plate 21 arranged on the side surface of the mouse cage 2 and used for controlling the mouse not to deviate after leaving the mouse cage 2, a door blocking device 4 arranged at the end part of the first baffle plate 21 and used for controlling the mouse to fall down, and a fixing device 3 arranged below the door blocking device 4 and used for fixing the mouse in a good position; the fixing device 3 comprises a first box 31 fixedly arranged below the first supporting frame 1 and used for containing mice, a lifting plate 32 arranged in the first box 31 and used for enabling the mice to move up and down, an air cylinder 311 arranged below the first box 31 and used for driving the lifting plate 32, a mouse foot leveling component 33 arranged on the lifting plate 32 and used for fixing limbs of the mice, two first control components 34 arranged on two sides of the first box 31 and used for being matched with the lifting plate 32 to drive the mouse foot leveling component 33, two second control components 35 arranged on two sides of the first box 31 and positioned above the first control components 34 and used for controlling the first control components 34 not to drive the mouse foot leveling component 33, two first fixing plates 36 arranged above the lifting plate 32 and positioned on two sides inside the first box 31 and used for pressing the mice, a third control component 37 arranged on two sides of the first box 31 and connected with the two first fixing plates 36 and used for being matched with the first control components 34 and used for controlling the first fixing plates 36 to move towards the middle position of the first box 31, and a third box 31 and a mouse killing component 37 arranged on the upper side of the first box 37 and used for being positioned on the neck of the first control components 32 and used for being fixed on the second control components 38; the mouse foot leveling component 33 comprises two first guide rods 331 connected to the first control component 34, two first guide blocks 332 slidably arranged on the first guide rods 331, a swinging structure 333 arranged on the first guide blocks 332 and connected to the lifting plate 32 for controlling the first guide blocks 332 to slide on the first guide rods 331, two third baffle plates 334 arranged on the lifting plate 32 for clamping a mouse, a second avoidance groove 335 arranged on the third baffle plates 334 for avoiding the swinging structure 333, an automatic clamping structure 336 arranged on the first guide blocks 332 for clamping limbs of the mouse, and a limit rod piece 337 arranged on the lifting plate 32 for being matched with the swinging structure 333; when the device is used, a mouse comes out of the mouse cage 2, reaches the door blocking device 4 along the first baffle 21, then falls onto the lifting plate 32, is clamped by the first control component 34, and cannot rotate, then the cylinder 311 drives the lifting plate 32 to move upwards, the lifting plate 32 moves upwards to cooperate with the first control component 34 to drive the first guide rod 331 to move towards the two sides of the first box 31, then the first guide rod 331 drives the first guide block 332 to move together, then the first guide block 332 moves towards the two sides of the first guide rod 331 under the action of the swinging structure 333, the first guide block 332 drives the automatic clamping structure 336 to move, the automatic clamping structure 336 clamps the limbs of the mouse and stretches towards the periphery of the mouse, the mouse is prone to the lifting plate 32, the swinging structure 333 is matched with the limit rod 337 when moving, the automatic clamping structure 336 presses the mouse on the periphery of the four limbs of the mouse to stretch, and then the first guide block 332 moves towards the two sides of the first guide rod 331, and then the first guide block 37 is matched with the first control component 36 when the first control component 35 is pressed down, and the first guide block 332 is not fixed, and the first guide block 36 moves towards the first control component 37 is not pressed together, and the first control component 36 is fixed.
Specifically, the tissue miRNA extraction in the step A is performed by using a tissue grinder on endometrial tissues, and then analyzing the result of small RNA sequencing.
Specifically, the tissue grinding instrument uses an instrument with better high-flux grinding effect.
Specifically, the automatic clamping structure 336 includes a fixing sleeve 3361 fixedly disposed on the automatic clamping structure 336, a fifth rotating shaft 3369 rotatably disposed in the fixing sleeve 3361, a third support frame 3362 fixedly disposed on the fifth rotating shaft 3369 and used for pushing the limbs of the mice, a second clamping plate 33622 disposed on the third support frame 3362 and used for clamping the limbs of the mice, a sliding rod 3363 slidably disposed on the third support frame 3362, a fourth rotating shaft 3364 rotatably disposed on the sliding rod 3363, a first clamping plate 3365 fixedly disposed on the fourth rotating shaft 3364 and used for clamping the limbs of the mice, a rotating member 3367 disposed above the fourth rotating shaft 3364 and used for controlling the first clamping plate 3365, a rope 3368 connected to the rotating member 3367 and the fixing sleeve 3361, and a guiding member 3366 disposed on the third support frame 3362 and used for guiding the rope 3368. The second clamping plate 33622 is provided with a plurality of first protrusions 33621; the first clamping plate 3365 is provided with a plurality of second protrusions 33651.
Specifically, the automatic clamping structure 336 further includes a second support plate 33631 fixedly disposed on the sliding rod 3363, and a fifth spring 33632 disposed between the second support plate 33631 and the third support frame 3362; the rotating component 3367 comprises a third gear 33671 fixedly arranged on the fourth rotating shaft 3364, a second rack gear 33672 meshed with the third gear 33671 and fixed on the third supporting frame 3362, and a second fixed block 33673 rotatably arranged above the fourth rotating shaft 3364 and fixedly connected with a rope 3368; the guiding component 3366 comprises a first fixed block 33661 fixedly arranged on the third supporting frame 3362, a sixth rotating shaft 33662 rotatably arranged on the first fixed block 33661, and a guiding wheel 33663 fixedly arranged on the sixth rotating shaft 33662; the number of teeth on the second rack 33672 is half that on the third gear 33671; the rope 3368 is wound around the guide wheel 33663.
Specifically, the swinging structure 333 includes a fifth link 3333 door device 4 fixed above the first guide block 332, a third link 3331 hinged to the first guide block 332, a fourth link 3332 fixed to the third link 3331, a fifth link 3333 hinged to the fourth link 3332, a first control plate 3335 fixed to the fourth link 3332 and contacting the fifth link 3333, a second support frame 3336 fixed to the fourth link 3332, a ninth guide bar 3337 slidably disposed in the second support frame 3336, a second control plate 3339 fixed to the ninth guide bar 3337 and abutting against the fifth link 3333, and a fourth spring 3338 disposed between the second support frame 3336 and the second control plate 3339; the stopper rod 337 is provided on a side surface of the fifth link 3333.
Specifically, the first control assembly 34 includes a plurality of first sliding grooves 347 formed on two sides of the first casing 31, a plurality of second sliding grooves 348 formed on the lifting plate 32, a first guide plate 341 slidably formed on the first sliding grooves 347, a first support plate 3413 formed on the first guide plate 341 and slidably formed in the second sliding grooves 348, a fifth sliding groove 3411 and a sixth sliding groove 3412 formed on the first guide plate 341, a third limiting block 3414 formed on the first support plate 3413 and used for being clamped on the second sliding grooves 348, a first rod 342 fixedly formed between the two first guide plates 341, a first connecting rod 343 hinged to the first rod 342, a second guide rod 345 fixedly formed on two sides of the first casing 31, a second guide plate 344 slidably formed on the second guide rod 345 and slidably formed in the sixth sliding groove 3412, and a third guide rod 346 fixedly formed on the second guide plate 344 and used for controlling the movement of the first guide plate 341; the second control assembly 35 includes a second fixing plate 351 fixedly disposed on the side of the first case 31 and distributed vertically, a second guide block 354 slidably disposed on the fourth guide bar 352, a first spring 353 disposed between the second fixing plate 351 and the second guide block 354, and a second rod 355 disposed on the second guide block 354 and hinged to the first link 343.
Specifically, the third control assembly 37 includes a fifth guide rod 371 fixedly disposed below the first support frame 1, a third guide plate 372 slidably disposed on the fifth guide rod 371, a third guide block 373 fixedly disposed on the third guide plate 372 and slidably connected to the third limiting block 3414, a second link 374 hinged to the third guide block 373, a fourth guide block 375 hinged to the other end of the third guide block 373, a sixth guide rod 376 fixedly disposed below the first support frame 1 and slidably connected to the fourth guide block 375 for allowing the fourth guide block 375 to move up and down, a seventh guide rod 377 fixedly disposed on the sixth guide rod 376 and the first fixing plate 36, a fifth guide block 378 fixedly disposed on the seventh guide rod 377, a second spring 379 disposed between the fifth guide block 378 and the first box 31 for allowing the first fixing plate 36 to reset, and a movement slot disposed on the first box 31 for allowing the seventh guide rod 377 to move up and down; the fourth control assembly 39 includes a third spring 391 disposed between the fourth guide block 375 and the first support frame 1 for making the fourth guide block 375 unable to move upward at the first time, a fifth guide plate 392 fixedly disposed on the fourth guide block 375, a third rod 393 fixedly disposed on the fifth guide plate 392, a sixth guide plate 394 fixedly disposed on the side of the first support frame 1 and disposed below the fifth guide plate 392, a third chute 395 disposed on the sixth guide plate 394 and cooperating with the third rod 393 for guiding the third rod 393, a second stopper 396 fixedly disposed above the third rod 393 for fixing the third rod 393 on the fifth guide plate 392, a sixth guide block 398 fixedly disposed below the third rod 393 and slidably cooperating with the third chute 395, and a seventh guide plate 397 fixedly disposed below the sixth guide plate 394 for supporting the third chute 395 so that the third chute 395 can move better; the third guide plate 372 and the fifth guide bar 371 are used to move the third guide block 373 only up and down.
Specifically, the rodenticide assembly 38 includes an eighth guide rod 385 fixedly disposed below the first box 31, a fourth guide plate 384 slidably disposed on the eighth guide rod 385, a first limiting block 386 disposed below the eighth guide rod 385 and used for clamping the fourth guide plate 384, a threaded sleeve 383 rotatably disposed on the fourth guide plate 384, a threaded rod 382 fixedly disposed in the threaded sleeve 383 in a threaded connection manner, a rodenticide plate 381 fixedly disposed on the threaded rod 382 and used for clamping a neck of a white mouse, and a bearing 387 fixedly disposed on the threaded sleeve 383 and disposed at an upper position and a lower position of the fourth guide plate 384 and used for clamping the threaded sleeve 383.
Specifically, the blocking devices 47 include a first rotating shaft 43 rotatably provided in the door blocking device 4, a second blocking plate 42 fixedly provided on the first rotating shaft 43 for blocking the first casing 31, a first avoidance groove 41 provided in the door blocking device 4 for avoiding the second blocking plate 42, a first connecting member 471 fixedly provided on the lifting plate 32, a first rack 47 fixedly provided on the first connecting member 471, a first gear 44 provided on the first rotating shaft 43, a second rotating shaft 46 rotatably provided at the first avoidance groove 41, a second gear 45 fixedly provided on the second rotating shaft 46 for meshing with the first rack 47 to control the rotation of the first gear 44, a first groove 48 provided on the second blocking plate 42, a torsion spring 49 provided in the first groove 48 for controlling the holding position of the second blocking plate 42, and a fourth runner 472 provided on the first casing 31 for allowing the first rack 47 to slide up and down.
The specific working flow of the invention is as follows: the mice to be tested are put out of the squirrel cage 2, the mice are moved up to the upper side of the second baffle 42 along the first baffle 21, the second baffle 42 is subjected to gravity, the second baffle 42 rotates, the mice drop into a cavity formed between the lifting plate 32 and the third baffle 334, the second baffle 42 resets under the action of the torsion spring 49, the cylinder 311 drives the lifting plate 32 to move upwards, the lifting plate 32 slides upwards in the first box 31, the lifting plate 32 moves upwards under the action of the third limit block 3414, one end of the first connecting rod 343 is hinged with the second rod 355, the second rod 355 does not move upwards, the first connecting rod 343 rotates in a small range under the action of the first guide plate 341, the first guide plate 341 is moved to both sides of the first box 31 under the action of the torsion spring 49, the first support plate 32 slides upwards in the first sliding groove 3412, the first guide block 331 slides to both sides of the first sliding block 331 and the second guide block 331, the first guide block 332 slides to both sides of the first sliding block 331 is moved upwards along the first sliding block 3414, the first sliding block 331 is moved to both sides of the first sliding block 331 is moved upwards along the first sliding block 3412, the first sliding block is moved together, the first guide block 331 is moved along the first sliding block 3433 is moved along the first sliding block 341, that is, the positions of the third link 3331, the fourth link 3332 and the fifth link 3333 are relatively unchanged, so that the rod composed of the three drives the first guide block 332 to move to two sides, then the fifth link 3333 impacts the limit rod 337 when moving, so that the swinging structure 333 is clamped between the third rotating shaft 3334 and the limit rod 337 and does not rotate any more, then the third link 3331 and the fourth link 3332 rotate around the hinging point with the fifth link 3333, the fifth link 3333 pushes the second control panel 3339 when rotating, the second control panel 3339 moves backwards, so that the second control panel 3339 can normally rotate, then the first guide block 332 is reset under the action of the fourth spring 3338, so that the rotating point is changed to increase the sliding range of the first guide block 332 on the first guide rod 331, then the first guide block 332 moves to two sides of the first guide rod 331, namely, the first guide block 332 moves around the center position of the mouse, then the third support frame 3362 on the first guide block 332 pushes the mouse around the center of the mouse, and then the mouse on the first guide block 332 pushes the fourth support frame 3362 to move around the mouse limbs;
At the same time, the first guide plate 341 moves upwards to drive the third guide block 373 to move upwards, then the second connecting rod 374 drives the fourth guide block 375 to move towards the inner side of the first box 31, then the sixth guide rod 376 moves together, the sixth guide rod 376 also moves towards the inner side of the first box 31 through the seventh guide rod 377 and the fifth guide block 378, then the first fixing plate 36 moves above the mice, the first fixing plate 36 reaches above the mice before the first guide block 332 impacts the limbs of the mice, then the first fixing plate 36 cannot move continuously, that is, the first fixing plate 36 cannot be driven to move continuously through the second connecting rod 374, at this time the second connecting rod 374 drives the fourth guide block 375 to move upwards on the automatic clamping structure 336, thereby ensuring that the first fixing plate 36 can reach the upper part of the mice before the first guide block 332 impacts the limbs of the mice, then the first fixing plate 36 is pressed on the backs and the necks of the mice when the first guide block 332 impacts the limbs of the mice, then the mice cannot break away upwards, then the third support frame 3362 on the first guide block 332 pushes the limbs of the mice to move, during pushing, the limbs of the mice are straightened to the periphery, so that the limbs of the mice are in an inclined state, the fifth rotating shaft 3369 on the third support frame 3362 rotates anticlockwise around the fixing sleeve 3361, the third support frame 3362 can be better attached to the limbs of the mice, then the fifth rotating shaft 3369 rotates, namely the ropes 3368 are stretched, so that the ropes 3368 pull the second fixing block 33673 to move, the second fixing block 33673 drives the fourth rotating shaft 3364 to move together, the third gear 33671 is meshed with the second rack 33672, so that the fourth rotating shaft 3364 rotates while moving, the fourth rotating shaft 3364 drives the third supporting frame 3362 to slide on the fixed sleeve 3361, the distance between the second supporting plate 33631 and the first bulge 33621 is shortened, the fourth rotating shaft 3364 rotates the first clamping plate 3365 to enable the first clamping plate 3365 to be parallel to the first bulge 33621, the third gear 33671 is disengaged from the second rack 33672, the rope 3368 is pulled continuously, the first clamping plate 3365 and the first bulge 33621 clamp the limbs of the mice, the mice are in a state of lying on the lifting plate 32, and the mice cannot exert force;
Then, the air cylinder 311 will continue to drive the lifting plate 32 to move upwards, at this time, the third guide rod 346 will abut against the second guide block 354, so as to drive the second guide block 354 to move upwards together, so that both ends of the second connecting rod 374 will move upwards together, at this time, the first guide block 332 will not continue to push the limbs of the mice around, then the mice are in a fixed state, then the mice move upwards on the lifting plate 32, then the first rack 47 on the lifting plate 32 moves upwards and is meshed with the second gear 45, the second gear 45 is meshed with the first gear 44, so that the first rotating shaft 43 drives the second baffle 42 to rotate for half a circle, so that the second baffle 42 is completely opened, the mice are exposed, and a series of treatments are performed on the mice;
when the mice are killed before treatment, the threaded rod 382 is required to be rotated, so that the threaded rod 382 and the threaded sleeve 383 are matched to drive the mouse killing plate 381 to move upwards, and then the mouse killing plate 381 abuts against the neck of the mice when the mice are compacted, so that the mice are killed.
It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Claims (8)
1. The automatic white mouse fixing equipment is characterized by comprising a first support frame (1), a mouse cage (2) arranged above the first support frame (1) and used for closing a white mouse, a cage door arranged on the mouse cage (2) and used for controlling the entrance and exit of the white mouse, a first baffle (21) arranged on the side surface of the mouse cage (2) and used for controlling the white mouse to leave the mouse cage (2) and not to deviate, a baffle door device (4) arranged at the end part of the first baffle (21) and used for controlling the falling of the white mouse, and a fixing device (3) arranged below the baffle door device (4) and used for fixing the white mouse; the fixing device (3) comprises a first box body (31) fixedly arranged below the first supporting frame (1) and used for containing mice, a lifting plate (32) arranged in the first box body (31) and used for enabling the mice to move up and down, an air cylinder (311) arranged below the first box body (31) and used for driving the lifting plate (32), a mouse foot leveling component (33) arranged on the lifting plate (32) and used for fixing limbs of the mice, two first control components (34) arranged on two sides of the first box body (31) and used for driving the mouse foot leveling component (33) in cooperation with the lifting plate (32), two second control components (35) arranged on two sides of the first box body (31) and positioned above the first control components (34) and used for controlling the first control components (34) not to drive the mouse foot leveling component (33), two first fixing plates (36) arranged above the lifting plate (32) and positioned at two sides inside the first box body (31) and used for pressing the mice, two first fixing plates (36) arranged on two sides of the first box body (31) and connected with the first box body (31) and used for fixing the first control components (37) to the first box body (36) at the middle position matched with the first control components (37), the fourth control components (39) are arranged on two sides of the first box body (31) and are positioned above the third control components (37) and used for controlling the first fixed plate (36) to move up and down, and the mouse killing components (38) are arranged on the lifting plate (32) and positioned on the neck of the mice; the mouse foot leveling component (33) comprises two first guide rods (331) connected to the first control component (34), two first guide blocks (332) arranged on the first guide rods (331) in a sliding mode, a swinging structure (333) arranged on the first guide blocks (332) and connected to the lifting plate (32) for controlling the first guide blocks (332) to slide on the first guide rods (331), two third baffle plates (334) arranged on the lifting plate (32) for clamping mice, a second avoidance groove (335) arranged on the third baffle plates (334) for avoiding the swinging structure (333), an automatic clamping structure (336) arranged on the first guide blocks (332) for clamping limbs of the mice, and a limit rod piece (337) arranged on the lifting plate (32) for being matched with the swinging structure (333); when the device is used, a mouse comes out of a mouse cage (2) and moves along a first baffle plate (21) to a door blocking device (4), then falls above a lifting plate (32) and is clamped by a first control component (34) to enable the mouse to be unable to rotate, then a cylinder (311) drives the lifting plate (32) to move upwards, the lifting plate (32) is matched with the first control component (34) to drive a first guide rod (331) to move towards the two sides of a first box body (31) when moving upwards, then the first guide rod (331) can drive a first guide block (332) to move together, then the first guide block (332) can move towards the two sides of the first guide rod (331) under the action of a swinging structure (333), the first guide block (332) can drive an automatic clamping structure (336) to move, the four limbs of the mouse are clamped and stretched around the mouse, the mouse is prone on the lifting plate (32), the swinging structure (333) is matched with the automatic clamping structure (336) to move around the four sides of the mouse, the four limbs of the mouse are matched with the first guide rod (331) when moving along the first guide rod (33) and the first guide block (33) is matched with the first guide block (35) to enable the first guide block (332) to move towards the two sides of the first guide block (331) under the action of the swinging structure (333), at this time, the first fixing plate (36) is already pressed against the mice, and the first fixing plate (36) drives the first fixing plate (36) and the third control assembly (37) to move upwards together under the action of the fourth control assembly (39).
2. The automatic mouse fixing device according to claim 1, wherein: the automatic clamping structure 336 comprises a fixed sleeve (3361) fixedly arranged on the automatic clamping structure (336), a fifth rotating shaft (3369) rotatably arranged in the fixed sleeve (3361), a third supporting frame (3362) fixedly arranged on the fifth rotating shaft (3369) and used for pushing the limbs of the mice, a second clamping plate (33622) arranged on the third supporting frame (3362) and used for clamping the limbs of the mice, a sliding rod (3363) slidably arranged on the third supporting frame (3362), a fourth rotating shaft (3364) rotatably arranged on the sliding rod (3363), a first clamping plate (3365) fixedly arranged on the fourth rotating shaft (3364) and used for clamping the limbs of the mice, a rotating part (3367) arranged above the fourth rotating shaft (3364) and used for controlling the first clamping plate (3365), a rope (3368) connected with the rotating part (3367) and the fixed sleeve (3361), and a guide part (3366) arranged on the third supporting frame (3362) and used for guiding the rope (3368); the second clamping plate (33622) is provided with a plurality of first bulges (33621); the first clamping plate (3365) is provided with a plurality of second protrusions (33651).
3. The automatic mouse fixing device according to claim 2, wherein: the automatic clamping structure (336) further comprises a second supporting plate (33631) fixedly arranged on the sliding rod (3363) and a fifth spring (33632) arranged between the second supporting plate (33631) and the third supporting frame (3362); the rotating component (3367) comprises a third gear (33671) fixedly arranged on a fourth rotating shaft (3364), a second rack (33672) meshed with the third gear (33671) and fixed on a third supporting frame (3362), and a second fixed block (33673) rotatably arranged above the fourth rotating shaft (3364) and fixedly connected with a rope (3368); the guide part (3366) comprises a first fixed block (33661) fixedly arranged on the third support frame (3362), a sixth rotating shaft (33662) rotatably arranged on the first fixed block (33661) and a guide wheel (33663) fixedly arranged on the sixth rotating shaft (33662); the number of teeth on the second rack (33672) is only half of the number of teeth on the third gear (33671); the rope (3368) is wound around the guide wheel (33663).
4. The automatic mouse fixing device according to claim 2, wherein: the swing structure (333) comprises a fifth connecting rod (3333) door blocking device (4) fixed above the first guide block (332), a third connecting rod (3331) hinged on the first guide block (332), a fourth connecting rod (3332) fixed on the third connecting rod (3331), a fifth connecting rod (3333) hinged on the fourth connecting rod (3332), a first control plate (3335) fixedly arranged on the fourth connecting rod (3332) and contacted with the fifth connecting rod (3333), a second support frame (3336) fixedly arranged on the fourth connecting rod (3332), a ninth guide rod (3337) slidingly arranged in the second support frame (3336), a second control plate (3339) fixedly arranged on the ninth guide rod (3337) and abutted against the fifth connecting rod (3333), and a fourth spring (3338) arranged between the second support frame (3336) and the second control plate (3339); the limit rod piece (337) is arranged on the side surface of the fifth connecting rod (3333).
5. The automatic mouse fixing device according to claim 2, wherein: the first control assembly (34) comprises a plurality of first sliding grooves (347) arranged on two side surfaces of the first box body (31), a plurality of second sliding grooves (348) arranged on the lifting plate (32), a first guide plate (341) arranged on the first sliding grooves (347) in a sliding manner, a first support plate (3413) arranged on the first guide plate (341) in the second sliding grooves (348) in a sliding manner, a fifth sliding groove (3411) and a sixth sliding groove (3412) arranged on the first guide plate (341), a third limit block (3414) arranged on the first support plate (3413) and used for being clamped on the second sliding groove (348), a first rod piece (342) fixedly arranged between the two first guide plates (341), a first connecting rod (343) hinged on the first rod piece (342), a second guide rod (345) fixedly arranged on two sides of the first box body (31), a second guide plate (344) arranged on the second guide plate (345) in the sixth sliding manner, and a third guide plate (344) fixedly arranged on the second guide plate (344) for being used for moving the first guide plate (346); the second control assembly (35) comprises a second fixing plate (351) fixedly arranged on the side face of the first box body (31) and distributed up and down, a second guide block (354) slidably arranged on the fourth guide rod (352), a first spring (353) arranged between the second fixing plate (351) and the second guide block (354), and a second rod piece (355) arranged on the second guide block (354) and hinged to the first connecting rod (343).
6. The automatic mouse fixing device of claim 5, wherein: the third control assembly (37) comprises a fifth guide rod (371) fixedly arranged below the first support frame (1), a third guide plate (372) fixedly arranged on the fifth guide rod (371), a third guide block (373) fixedly arranged on the third guide plate (372) and connected with a third limit block (3414) in a sliding manner, a second connecting rod (374) hinged to the third guide block (373), a fourth guide block (375) hinged to the other end of the third guide block (373), a sixth guide rod (376) fixedly arranged below the first support frame (1) and connected with the fourth guide block (375) in a sliding manner and used for enabling the fourth guide block (375) to move up and down, a seventh guide rod (377) fixedly arranged on the sixth guide rod (376) and the first fixing plate (36), a fifth guide block (378) fixedly arranged on the seventh guide rod (377), a second spring (379) arranged between the fifth guide block (378) and the first box (31) and used for enabling the first fixing plate (36) to reset, and a seventh box (31) to be arranged on the seventh guide rod (377) for enabling the seventh guide rod (377) to move up and down; the fourth control assembly (39) comprises a third spring (391) arranged between the fourth guide block (375) and the first support frame (1) and used for enabling the fourth guide block (375) not to move upwards at the first time, a fifth guide plate (392) fixedly arranged on the fourth guide block (375), a third rod piece (393) fixedly arranged on the fifth guide plate (392), a sixth guide plate (394) fixedly arranged on the side surface of the first support frame (1) and positioned below the fifth guide plate (392), a third chute (395) arranged on the sixth guide plate (394) and matched with the third rod piece (393) for enabling the third rod piece (393) to guide, a second limiting block (396) fixedly arranged above the third rod piece (393) and used for fixing the third rod piece (393) on the fifth guide plate (392), a sixth guide block (398) fixedly arranged below the third rod piece (393) and matched with the third chute (395) in a sliding manner, and a seventh chute (395) fixedly arranged below the sixth guide plate (395) and used for supporting the third rod piece (393) to move better; the third guide plate (372) and the fifth guide rod (371) are used for enabling the third guide block (373) to only move up and down.
7. The automatic mouse fixing device according to claim 1, wherein: the mouse killing assembly (38) comprises an eighth guide rod (385) fixedly arranged below the first box body (31), a fourth guide plate (384) arranged on the eighth guide rod (385) in a sliding mode, a first limiting block (386) arranged below the eighth guide rod (385) and used for clamping the fourth guide plate (384), a threaded sleeve (383) rotationally arranged on the fourth guide plate (384), a threaded rod (382) fixedly arranged in the threaded sleeve (383) in a threaded connection mode, a mouse killing plate (381) fixedly connected to the threaded rod (382) and used for clamping the neck of a mouse, and a bearing (387) fixedly arranged on the threaded sleeve (383) and located at the upper position and the lower position of the fourth guide plate (384) and used for clamping the threaded sleeve (383).
8. The automatic mouse fixing device according to claim 1, wherein: the shutter device (4) comprises a first rotating shaft (43) which is arranged in the shutter device (4) in a rotating mode, a second baffle plate (42) which is fixedly arranged on the first rotating shaft (43) and used for blocking the first box body (31), a first avoidance groove (41) which is arranged in the shutter device (4) and used for avoiding the second baffle plate (42), a first connecting piece (471) which is fixedly arranged on the lifting plate (32), a first rack (47) which is fixedly arranged on the first connecting piece (471), a first gear (44) which is arranged on the first rotating shaft (43), a second rotating shaft (46) which is arranged on the first avoidance groove (41) in a rotating mode, a second gear (45) which is fixedly arranged on the second rotating shaft (46) and used for meshing with the first rack (47) to control the rotation of the first gear (44), a first groove (48) which is arranged on the second baffle plate (42), a torsion spring (49) which is arranged in the first groove (48) and used for controlling the holding position of the second baffle plate (42), and a fourth chute (472) which is arranged on the first box body (31) and used for enabling the first rack (47) to slide up and down.
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| CN202110761587.8A CN113584180B (en) | 2021-07-06 | 2021-07-06 | Application of exosome miRNA derived from endometrium in embryo implantation |
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| CN202110761587.8A CN113584180B (en) | 2021-07-06 | 2021-07-06 | Application of exosome miRNA derived from endometrium in embryo implantation |
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| CN113584180B true CN113584180B (en) | 2024-02-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103571843A (en) * | 2013-10-11 | 2014-02-12 | 安徽瀚海博兴生物技术有限公司 | Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation |
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| CN110038024A (en) * | 2019-03-27 | 2019-07-23 | 浙江大学 | Application of the mmu-miR-192-5p in the drug that preparation inhibits embryo nidation |
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| US10253374B2 (en) * | 2015-05-15 | 2019-04-09 | Wisconsin Alumni Research Foundation | Non-invasive assays for embryo quality |
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| CN103571843A (en) * | 2013-10-11 | 2014-02-12 | 安徽瀚海博兴生物技术有限公司 | Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation |
| GB201501302D0 (en) * | 2015-01-27 | 2015-03-11 | Ostara Biomedical Ltd | Embryo implantation |
| CN206586948U (en) * | 2016-11-22 | 2017-10-27 | 况春燕 | A kind of mice embryonic surface electrocardiogram operating desk |
| CN210095982U (en) * | 2019-02-15 | 2020-02-21 | 重庆医科大学附属儿童医院 | Multi-functional laboratory mouse fixing device |
| CN110038024A (en) * | 2019-03-27 | 2019-07-23 | 浙江大学 | Application of the mmu-miR-192-5p in the drug that preparation inhibits embryo nidation |
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