CN113583932A - Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof - Google Patents
Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof Download PDFInfo
- Publication number
- CN113583932A CN113583932A CN202110858894.8A CN202110858894A CN113583932A CN 113583932 A CN113583932 A CN 113583932A CN 202110858894 A CN202110858894 A CN 202110858894A CN 113583932 A CN113583932 A CN 113583932A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- medium
- acetic acid
- escherichia coli
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for inhibiting fermentation of escherichia coli and byproduct acetic acid and a fermentation process thereof, wherein the fermentation culture medium comprises the following components: lactose 1-5% (g/mL), KH2PO40.01‑0.2％(g/mL)、K2HPO4·3H2O 0.01‑0.2％(g/mL)、MgSO4·7H20.01-0.05% (g/mL) of O, 0.1-1% (g/mL) of yeast powder, 0.1-1% (g/mL) of ammonium chloride, 0.1-1% (g/mL) of urea, 0.01-0.1% (g/mL) of ferric chloride or 0.01-0.1% (g/mL) of ferrous sulfate, 1-5mL/L of inducer, the balance of water, and the pH value of 6.5-7.5; a supplemented medium: 25-100g/L of yeast powder, 25-100g/L of peptone and 600g/L of glycerol. The acetic acid accumulation in the fermentation process is reduced to below 1g/L, and the fermentation level can be improved. The same fermentation medium is not added with Fe as an acetic acid inhibitorThe contrast is obvious, the fermentation enzyme activity is stable, the fermentation tank change caused by high acetic acid byproduct is reduced, the raw material loss is less, the economic value is high, and the method is suitable for industrial production.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a culture medium for inhibiting byproduct acetic acid by escherichia coli fermentation and a fermentation process thereof.
Background
Escherichia coli is a commonly used host bacterium in genetic engineering and is widely applied to foreign protein expression. However, some harmful byproducts are produced in the fermentation and culture process of the escherichia coli, and acetic acid is harmful and most commonly used. Acetic acid affects the growth of E.coli and the expression of target protein, and the production efficiency is seriously affected. Therefore, in the high-density culture of recombinant E.coli, the reduction of acetic acid production during fermentation can improve the production efficiency. At present, common measures for reducing byproduct acetic acid by escherichia coli fermentation are controlling dissolved oxygen level, initial sugar concentration, a limited fed-batch mode of feeding and the like, and the measures have very low effect on escherichia coli with more byproduct acetic acid in the fermentation control process. Therefore, the production efficiency in the high-density culture process is low, the utilization rate of raw materials is low, and the economic effect is poor.
Disclosure of Invention
The invention aims to provide a culture medium for inhibiting byproduct acetic acid in escherichia coli fermentation and a fermentation process thereof. The fermentation enzyme has stable activity, reduces the fermentation tank change caused by high acetic acid byproduct, has less raw material loss and high economic value, is suitable for industrial production, and can solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a culture medium for inhibiting by-product acetic acid in Escherichia coli fermentation comprises fermentation cultureThe medium and the supplementary medium are characterized in that the fermentation medium comprises the following components: lactose 1-5% (g/mL), KH2PO4 0.01-0.2％(g/mL)、K2HPO4·3H2O 0.01-0.2％(g/mL)、MgSO4·7H20.01-0.05% (g/mL) of O, 0.1-1% (g/mL) of yeast powder, 0.1-1% (g/mL) of ammonium chloride, 0.1-1% (g/mL) of urea, 0.01-0.1% (g/mL) of ferric chloride or 0.01-0.1% (g/mL) of ferrous sulfate, 1-5mL/L of inducer, the balance of water, and the pH value of 6.5-7.5; a supplemented medium: 25-100g/L of yeast powder, 25-100g/L of peptone and 600g/L of glycerol.
Further, the inducer is an IPTG inducer.
Further, the fermentation medium consists of: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H20.01% (g/mL) of O, 0.5% (g/mL) of yeast powder, 0.5% (g/mL) of ammonium chloride, 0.5% (g/mL) of urea, 0.05% (g/mL) of ferric chloride or 0.05% (g/mL) of ferrous sulfate, 3.5mL/L of inducer and the balance of water, wherein the pH value is 6.5; a supplemented medium: 50g/L of yeast powder, 50g/L of peptone and 400g/L of glycerol.
Further, the fermentation medium consists of: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H2O0.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, water in balance, pH 7; a supplemented medium: 100g/L of yeast powder and 400g/L of glycerol.
Further, the fermentation medium consists of: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H2O0.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, water in balance, pH 7.5.
The invention also provides another technical scheme: a fermentation process of a culture medium for inhibiting byproduct acetic acid by escherichia coli fermentation comprises the following steps:
step 1: preparing a first-level seed solution: culturing Escherichia coli from a flat plate, inoculating the Escherichia coli to a first-stage seed liquid shake flask with a culture medium liquid loading amount of 40mL, carrying out shake cultivation at 25-35 ℃ for 12-48h, and carrying out shaking table rotation speed of 150-300rpm to obtain a first-stage seed liquid;
step 2: preparing a secondary seed solution: inoculating the cultured primary seeds into a secondary seed liquid shake flask with the culture medium liquid loading amount of 250mL according to the inoculation amount of 5-20%, carrying out shake cultivation for 12-48h at the temperature of 25-35 ℃ and the rotation speed of a shaking table of 150 plus 300rpm to obtain a secondary seed liquid;
and step 3: fermentation culture: adding 1-5L of fermentation medium into a 10L fermentation tank;
and 4, step 4: sterilizing the culture tank at 121 ℃ for 10-30min, inoculating the secondary seed liquid into a fermentation tank with the inoculation amount of 5-20%, fermenting at 25-35 ℃ for 72-96h, regulating and controlling the fermentation feeding according to the specific growth rate in the culture process, wherein the specific growth rate is controlled to be 0.01-0.2, the air flow rate in the fermentation process is 2-5L/min, the dissolved oxygen is controlled to be 20-50%, the pH value is controlled to be 6.5-7.5, supplementing an inducer when the OD600 reaches 50-70, sampling every 4-6h after induction, and stopping reaction after the enzyme activity is reduced.
Compared with the prior art, the invention has the beneficial effects that:
in order to reduce the byproduct acetic acid generated in the fermentation process of escherichia coli, the byproduct acetic acid inhibitor is added with metal ions, namely ferric chloride or ferrous sulfate and is used as an acetic acid production inhibitor in the fermentation process, the acetic acid production inhibitor is prepared into a specific fermentation culture medium, the specific fermentation process conditions are used for regulation and control, the generation of the byproduct acetic acid in the fermentation process of escherichia coli can be successfully controlled finally, the acetic acid accumulation in the fermentation process is reduced to be below 1g/L, and the fermentation level can be improved. Compared with the result of not adding Fe serving as an acetic acid inhibitor in a fermentation medium, the method has the advantages of stable fermentation enzyme activity, reduction of fermentation tank switching caused by high acetic acid byproduct, less raw material loss, high economic value and suitability for industrial production.
Drawings
FIG. 1 is a flow diagram of a fermentation process of the present invention;
FIG. 2 is a graph showing a fermentation process in example 1 of the present invention;
FIG. 3 is an electrophoretogram of a protein according to example 1 of the present invention;
FIG. 4 is a graph showing a fermentation process in example 2 of the present invention;
FIG. 5 is an electrophoretogram of a protein according to example 2 of the present invention;
FIG. 6 is a graph showing a fermentation process in example 3 of the present invention;
FIG. 7 is an electrophoretogram of a protein according to example 3 of the present invention;
FIG. 8 is a graph showing the amount of alkali supplementation during the fermentation process in example 3 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The fermentation process flow is shown in figure 1, and the fermentation process of the culture medium for inhibiting the byproduct acetic acid by escherichia coli fermentation comprises the following steps:
(1) preparing a first-level seed solution: culturing escherichia coli from a flat plate, inoculating the escherichia coli into a first-stage seed liquid shake flask with a culture medium liquid loading amount of 40mL, carrying out shake cultivation for 12h at 35 ℃ in a shaking table with a shaking table rotating speed of 200rpm to obtain a first-stage seed liquid;
(2) preparing a secondary seed solution: inoculating the cultured primary seeds into a secondary seed liquid shake flask with the culture medium liquid loading amount of 250mL according to the inoculation amount of 10%, carrying out shake cultivation for 12h at 35 ℃ by using a shaking table and at the shaking table rotating speed of 200rpm to obtain a secondary seed liquid;
(3) fermentation culture: 2L of fermentation medium was added to a 10L fermenter, the fermentation medium consisting of: lactose 2.5% (g/mL), KH2PO40.2% (g/mL), K2HPO 4.3H 2O 0.2% (g/mL), MgSO 4.7H 2O 0.01.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.05% (g/mL) or ferrous sulfate 0.05% (g/mL), inducer 3.5mL/L, balance water, pH 6.5;
a supplemented medium: 50g/L of yeast powder, 50g/L of peptone and 400g/L of glycerol.
(4) Sterilizing the solid tank at 121 ℃ for 30min, inoculating the secondary seed liquid into the fermentation tank with the inoculation amount of 20%, fermenting for 96h at 35 ℃, and regulating and controlling the specific growth rate of fermentation feeding materials in the culture process according to the specific growth rate, wherein the specific growth rate is controlled to be 0.02, the air flow rate in the fermentation process is 3L/min, and the dissolved oxygen is controlled to be 30%.
Controlling the pH value to be 7, supplementing an inducer when the OD600 reaches 60, sampling every 4-6h after induction, stopping reaction after the OD is reduced, and finally putting the tank until the OD reaches 230.
The fermentation process curve of this example is shown in FIG. 2, and the protein electrophoresis is shown in FIG. 3.
Example 2
A fermentation process of a culture medium for inhibiting byproduct acetic acid by escherichia coli fermentation comprises the following steps:
(1) preparing a first-level seed solution: culturing escherichia coli from a flat plate, inoculating the escherichia coli into a first-stage seed liquid shake flask with a culture medium liquid loading amount of 40mL, carrying out shake cultivation for 12h at 35 ℃ in a shaking table with a shaking table rotating speed of 200rpm to obtain a first-stage seed liquid;
(2) preparing a secondary seed solution: inoculating the cultured primary seeds into a secondary seed liquid shake flask with the culture medium liquid loading amount of 250mL according to the inoculation amount of 10%, carrying out shake cultivation for 12h at 35 ℃ by using a shaking table and at the shaking table rotating speed of 200rpm to obtain a secondary seed liquid;
(3) fermentation culture: 2L of fermentation medium was added to a 10L fermenter, the fermentation medium consisting of: lactose 2.5% (g/mL), KH2PO40.2% (g/mL), K2HPO 4.3H 2O 0.2% (g/mL), MgSO 4.7H 2O 0.01.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, balance water, pH 7;
the feed culture medium comprises: 100g/L of yeast powder and 400g/L of glycerol.
(4) Sterilizing the solid tank at 121 ℃ for 30min, inoculating the secondary seed liquid into a fermentation tank with the inoculation amount of 20%, fermenting for 96h at 35 ℃, regulating and controlling the fermentation feeding according to the specific growth rate in the culture process, wherein the specific growth rate is controlled to be 0.02, the air flow rate in the fermentation process is 3L/min, the dissolved oxygen is controlled to be 30%, the pH value is controlled to be 7, supplementing an inducer when the OD600 reaches 60, sampling every 4-6h after induction, and stopping reaction after the OD is reduced. Finally the can OD reached 240.
The fermentation process curve of this example is shown in FIG. 4, and the protein electrophoresis is shown in FIG. 5.
Example 3
A fermentation process of a culture medium for inhibiting byproduct acetic acid by escherichia coli fermentation comprises the following steps:
(1) preparing a first-level seed solution: culturing escherichia coli from a flat plate, inoculating the escherichia coli into a first-stage seed liquid shake flask with a culture medium liquid loading amount of 40mL, carrying out shake cultivation for 12h at 35 ℃ in a shaking table with a shaking table rotating speed of 200rpm to obtain a first-stage seed liquid;
(2) preparing a secondary seed solution: inoculating the cultured primary seeds into a secondary seed liquid shake flask with the culture medium liquid loading amount of 250mL according to the inoculation amount of 10%, carrying out shake cultivation for 12h at 35 ℃ by using a shaking table and at the shaking table rotating speed of 200rpm to obtain a secondary seed liquid;
(3) fermentation culture: 2L of fermentation medium was added to a 10L fermenter, the fermentation medium consisting of: lactose 2.5% (g/mL), KH2PO40.2% (g/mL), K2HPO 4.3H 2O 0.2% (g/mL), MgSO 4.7H 2O 0.01.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, balance water, pH 7.5;
a supplemented medium: 100g/L of yeast powder and 400g/L of glycerol.
(4) Sterilizing the solid tank at 121 ℃ for 30min, inoculating the secondary seed liquid into a fermentation tank with the inoculation amount of 20%, fermenting for 96h at the temperature of 35 ℃, regulating and controlling the fermentation feeding according to the specific growth rate in the culture process, controlling the specific growth rate at 0.02, controlling the air flow rate at 3L/min and the dissolved oxygen at 30% and the pH value at 7 in the fermentation process, supplementing the inducer when the OD600 reaches 60, sampling every 4-6h after induction, and stopping reaction after the OD is reduced. Finally the can OD reached 210.
(5) Repeating the above four steps, but changing the components of the culture medium in the step (3), wherein ferric chloride or ferrous sulfate is abandoned, and fermentation culture is carried out, other conditions are not changed, and the OD of the final tank is only 80.
The fermentation process curve of this example is shown in FIG. 6, the protein electrophoresis is shown in FIG. 7, and the alkali supplementation amount during the fermentation process is shown in FIG. 8, which laterally illustrates the acetic acid production.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (6)
1. A culture medium for inhibiting byproduct acetic acid in escherichia coli fermentation comprises a fermentation culture medium and a supplemented culture medium, and is characterized in that the fermentation culture medium comprises the following components: lactose 1-5% (g/mL), KH2PO4 0.01-0.2％(g/mL)、K2HPO4·3H2O 0.01-0.2％(g/mL)、MgSO4·7H20.01-0.05% (g/mL) of O, 0.1-1% (g/mL) of yeast powder, 0.1-1% (g/mL) of ammonium chloride, 0.1-1% (g/mL) of urea, 0.01-0.1% (g/mL) of ferric chloride or 0.01-0.1% (g/mL) of ferrous sulfate, 1-5mL/L of inducer, the balance of water, and the pH value of 6.5-7.5; a supplemented medium: 25-100g/L of yeast powder, 25-100g/L of peptone and 600g/L of glycerol.
2. The medium for inhibiting acetic acid as a byproduct of Escherichia coli fermentation according to claim 1, wherein the inducer is IPTG inducer.
3. The medium for suppressing acetic acid byproduct from fermentation of Escherichia coli according to claim 1, wherein the fermentation medium comprises: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H20.01% (g/mL) of O, 0.5% (g/mL) of yeast powder, 0.5% (g/mL) of ammonium chloride, 0.5% (g/mL) of urea, 0.05% (g/mL) of ferric chloride or 0.05% (g/mL) of ferrous sulfate, 3.5mL/L of inducer and the balance of water, wherein the pH value is 6.5; a supplemented medium: 50g/L of yeast powder, 50g/L of peptone and 400g/L of glycerol.
4. The medium for suppressing acetic acid byproduct from fermentation of Escherichia coli according to claim 1, wherein the fermentation medium comprises: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H2O0.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, water in balance, pH 7; a supplemented medium: 100g/L of yeast powder and 400g/L of glycerol.
5. The medium for suppressing acetic acid byproduct from fermentation of Escherichia coli according to claim 1, wherein the fermentation medium comprises: lactose 2.5% (g/mL), KH2PO4 0.2％(g/mL)、K2HPO4·3H2O 0.2％(g/mL)、MgSO4·7H2O0.01% (g/mL), yeast powder 0.5% (g/mL), ammonium chloride 0.5% (g/mL), urea 0.5% (g/mL), ferric chloride 0.75% (g/mL) or ferrous sulfate 0.75% (g/mL), inducer 3.5mL/L, water in balance, pH 7.5.
6. The fermentation process of the culture medium for inhibiting the byproduct acetic acid in the fermentation of Escherichia coli according to claim 1, comprising the steps of:
step 1: preparing a first-level seed solution: culturing Escherichia coli from a flat plate, inoculating the Escherichia coli to a first-stage seed liquid shake flask with a culture medium liquid loading amount of 40mL, carrying out shake cultivation at 25-35 ℃ for 12-48h, and carrying out shaking table rotation speed of 150-300rpm to obtain a first-stage seed liquid;
step 2: preparing a secondary seed solution: inoculating the cultured primary seeds into a secondary seed liquid shake flask with the culture medium liquid loading amount of 250mL according to the inoculation amount of 5-20%, carrying out shake cultivation for 12-48h at the temperature of 25-35 ℃ and the rotation speed of a shaking table of 150 plus 300rpm to obtain a secondary seed liquid;
and step 3: fermentation culture: adding 1-5L of fermentation medium into a 10L fermentation tank;
and 4, step 4: sterilizing the culture tank at 121 ℃ for 10-30min, inoculating the secondary seed liquid into a fermentation tank with the inoculation amount of 5-20%, fermenting at 25-35 ℃ for 72-96h, regulating and controlling the fermentation feed according to the specific growth rate in the culture process, controlling the specific growth rate at 0.01-0.2, controlling the air flow rate at 2-5L/min in the fermentation process, controlling the dissolved oxygen at 20-50%, controlling the pH value at 6.5-7.5, supplementing an inducer when the OD600 reaches 50-70, sampling every 4-6h after induction, and stopping reaction after the enzyme activity is reduced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110858894.8A CN113583932A (en) | 2021-07-28 | 2021-07-28 | Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110858894.8A CN113583932A (en) | 2021-07-28 | 2021-07-28 | Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113583932A true CN113583932A (en) | 2021-11-02 |
Family
ID=78251230
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110858894.8A Pending CN113583932A (en) | 2021-07-28 | 2021-07-28 | Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113583932A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180112255A (en) * | 2017-04-03 | 2018-10-12 | 한국생명공학연구원 | Streptomyces sp. m19-1 having high productivity of paromomycin and culture medium composition for mass production of streptomyces sp. m19-1 |
| CN110257450A (en) * | 2019-07-15 | 2019-09-20 | 安徽瑞邦生物科技有限公司 | A kind of method of bacillus subtilis nitrile hydratase production high-purity niacinamide |
-
2021
- 2021-07-28 CN CN202110858894.8A patent/CN113583932A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180112255A (en) * | 2017-04-03 | 2018-10-12 | 한국생명공학연구원 | Streptomyces sp. m19-1 having high productivity of paromomycin and culture medium composition for mass production of streptomyces sp. m19-1 |
| CN110257450A (en) * | 2019-07-15 | 2019-09-20 | 安徽瑞邦生物科技有限公司 | A kind of method of bacillus subtilis nitrile hydratase production high-purity niacinamide |
Non-Patent Citations (4)
| Title |
|---|
| YE HENG LIM等: "Rapid Evaluation and Optimization of Medium Components Governing Tryptophan Production by Pediococcus acidilactici TP-6 Isolated from Malaysian Food via Statistical Approaches", 《MOLECULES》 * |
| 张嗣良，储炬编著: "《多尺度微生物过程优化》", 30 September 2003 * |
| 陈理等: "重组大肠杆菌高密度发酵工艺进展", 《海峡药学》 * |
| 黄方一主编: "《发酵工程》", 31 January 2006 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2249061C (en) | Protein production process | |
| US20030175951A1 (en) | Methods for growing mammalian cells in vitro | |
| CN104404097B (en) | A kind of fermentation method for producing of high-yield glutamine | |
| CN113186147B (en) | Fermentation method for improving production of porcine myoglobin by pichia pastoris engineering bacteria | |
| JP6300121B2 (en) | High concentration culture method of E. coli cells | |
| Møller et al. | Production of fungal α-amylase by Saccharomyces kluyveri in glucose-limited cultivations | |
| CN102168118B (en) | Method for increasing fermentation output of tryptophan | |
| CN103627743A (en) | Method for improving fermentation yield of L-tryptophan | |
| CN102604904B (en) | Production method of glucose dehydrogenase | |
| CN113583932A (en) | Culture medium for inhibiting byproduct acetic acid by fermentation of escherichia coli and fermentation process thereof | |
| CN104561199B (en) | A kind of method that porcine alpha-IFN is stably produced using recombinant yeast pichia pastoris | |
| CN101139570A (en) | High-density fermentation method for HPV L1 protein prokaryotic expression | |
| CN105316371B (en) | A method of for improving tryptophan fermentation yield | |
| KR100405944B1 (en) | Protein manufacturing method | |
| CN107475223B (en) | Production method of acidic cellulase | |
| CN103627775A (en) | Method for improving fermentation production efficiency of 2-keto-L-gulonic acid | |
| CN111996225A (en) | Fermentation method for producing high-activity recombinant human interferon alpha 2b by using microorganisms | |
| CN109706206B (en) | Method for producing delicious peptide by segmented feeding fermentation | |
| CN112522338A (en) | Method for improving fermentation yield of L-tryptophan | |
| CN112029810A (en) | Culture medium for producing insulin glargine and fermentation method | |
| CN115125171B (en) | High-temperature culture process of glutamic acid fermentation bacteria | |
| CN103898142A (en) | Method for improving expression quantity of pichia pastoris foreign protein by using mercaptan peroxidase | |
| CN1231572C (en) | Fermentation process for reforming yeast of liking for carbinol | |
| CN112391431B (en) | Fermentation medium and fermentation method for recombinant leukocyte inhibitory factor and hirudin chimeric protein | |
| CN115651883A (en) | Sialyllactose fermentation medium additive and application thereof in improving sialyllactose production capability of microorganisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |