CN113583906B - Application of pseudomonas B5 in algae removal - Google Patents
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Abstract
The invention provides application of pseudomonas B5 in algae removal. Pseudomonas B5 from North Svalbard island is used as an experimental material, and the number of algae cells and the OD of algae liquid are measured 720 And chlorophyll a content, the pseudomonas B5 has obvious algicidal effect on the microcystis aeruginosa. After the pseudomonas B5 cultured by the novel culture medium is treated for 14 days, the algicidal rate reaches 87%. The algae dissolving mode of the pseudomonas B5 is indirect algae dissolving, the algae dissolving activity of the sterile filtrate is similar to that of the original bacterial liquid, the sterile filtrate treated at high temperature still has higher algae dissolving effect, and the pseudomonas B5 is presumed to exert algae dissolving effect through secretion of biological macromolecules and heat-resistant micromolecules.
Description
Technical Field
The invention relates to application of pseudomonas B5 in algae removal, and belongs to the field of environmental protection.
Background
Water bloom (HBs) is classified into fresh water bloom and ocean red tide, and is an aquatic ecological anomaly. When domestic sewage, industrial sewage and agricultural sewage containing a large amount of nutrients flow into the ocean or lake, red tide organisms (phytoplankton, protozoa or bacteria) and water bloom organisms (blue algae, green algae or diatoms) are rapidly propagated to form algal bloom under specific environmental conditions (such as ocean current, temperature, sealing degree of the sea area and the like).
Water bloom has a serious impact on the ecosystem, aquaculture and human health in a number of ways. Rapid excessive proliferation of algae can release secondary metabolites and toxins, or affect symbionts and alter food network dynamics through the physical structure of the algae cells or accumulated biomass, thereby jeopardizing the aquatic ecosystem. Water bloom can also lead to reduced water clarity, reduced aesthetic value, consumption of oxygen, production of toxins, and thus death of large numbers of aquatic organisms, ultimately affecting the biodiversity of the aquatic organisms. Harmful water bloom can pollute drinking water and aquatic products, not only destroy the structure and the function of an aquatic ecological system, but also cause different degrees of injury to human beings through a food chain, and even cause death.
Common water bloom prevention and control methods include physical, chemical and biological methods. The physical method comprises the technologies of mechanical salvage, ultrasonic treatment, membrane filtration, adsorption and the like; the chemical rule is to remove the water bloom by metal, photosensitizer, herbicide and other chemicals; biological methods refer to the use of aquatic animals, aquatic plants, microorganisms and the like in natural ecology to degrade algae cells, thereby achieving the purpose of removing water bloom. The physical and chemical methods can remove algal cells to some extent, but are costly and prone to secondary pollution [ Jiao Yankai, yan Xiaojun, li Xiaobing. Development of algicidal bacteria and algicidal compounds, industrial microorganisms, 2018,48 (4): 56-62]. Biological algae control is the most potential algae control method at present, wherein algae-lysing microorganisms have the characteristics of easy propagation, good algae-lysing effect, strong specificity and the like [ Sun R, sun P, zhang J, esquirol-Elizondo S, wu Y.Microorganisms-based methods for harmful algal blooms control: A review.Bioresource technology.2018, 248 (Pt B): 12-20].
The algicidal microorganisms include algicidal bacteria, algicidal fungi, algicidal viruses, algicidal protist, etc., and have different algicidal modes for algae. Algicidal bacteria are capable of inhibiting algae growth, or killing algae and even lysing algae cells, by direct or indirect means. Algae-lysing fungi lyse algae mainly by secreting antibiotics or parasitizing in algae cells. Algicidal viruses, commonly referred to as phagosomes, cause lysis of host algal cells, primarily by direct infection, and exert an algae control effect. Algae-dissolving protists control the population density of algae mainly through ingestion, and ingestion of algae tends to have certain preference, so that the change of the algae population structure is influenced, the succession direction of an algae community is changed, and the desquamation of water bloom is influenced to a certain extent.
At present, microorganisms used for actual algae control mainly include photosynthetic bacteria, lactic acid bacteria, nitrifying bacteria, denitrifying bacteria, pseudomonas and the like, and other microorganisms are often used for assisting bacteria in algae dissolving.
The algae dissolving mode of the algae dissolving bacteria includes direct algae dissolving, indirect algae dissolving and direct and indirect synergistic algae dissolving. Direct algicidal is that algicidal bacteria directly attack algae cells, directly contact with the surfaces of the algae cells, and even invade the algae cells to cause cracking and death of the algae cells. The thallus of partial algicidal bacteria has special structure, and can be made to be fast near to algae cell and adsorbed to cell surface or invaded inside algae cell. Indirect algae lysis involves 3 modes: secretion of algicidal substances, competition of nutrients with algae, and inhibition of photosynthesis of algae. Specific or non-specific extracellular substances secreted by algicidal bacteria during metabolism, such as proteins, alkaloids, cyclic peptides, fatty acids, etc., can disrupt the structure of the algae cells and cause their lytic death. Certain algicidal bacteria can inhibit algae by competing for limited nutrients. For example, when the nitrogen/phosphorus ratio is increased, the nutrient absorption rate of some algicidal bacteria is significantly higher than that of algae, which in turn results in inhibition of microalgae growth. The algae-dissolving bacteria can also form a layer of bacteria adhesive film on the surface of the water body to prevent light transmission and gas exchange, so that the underwater illumination is seriously insufficient, and normal photosynthesis of algae is prevented.
Disclosure of Invention
The invention aims to provide a novel culture method and application of pseudomonas B5.
The novel culture method and the application of the pseudomonas B5 provided by the invention are as follows: pseudomonas B5 is cultured by adopting a novel culture medium, and the application of the Pseudomonas B5 cultured by adopting the novel culture medium in algae removal is provided.
Pseudomonas sp.B5 was the Pseudomonas strain, and the subject group was isolated from soil samples of the North Svalbard island in 2019.
The novel culture medium consists of the following components: NH (NH) 4 H 2 PO 4 1g，MgSO 4 ·7H 2 O0.2 g, trisodium citrate 0.5g, K 2 HPO 4 1g, add dH 2 O was constant to 1,000mL.
The cultivation operation is as follows: taking out Pseudomonas sp.B5 preserved at-20deg.C, streaking 10 μl of bacterial liquid on LB solid medium, culturing in a constant temperature incubator for 24 hr, picking single colony in 60mL of the new culture medium, and culturing for 24 hr.
The algae dissolving mode is indirect algae dissolving.
The invention takes pseudomonas B5 from North Svalbard island as an experimental material, and determines the quantity of algae cells and the OD of algae liquid 720 And chlorophyll a content, the pseudomonas B5 has obvious algicidal effect on the microcystis aeruginosa. After the pseudomonas B5 cultured by the novel culture medium is treated for 14 days, the algicidal rate reaches 87%. The algae dissolving mode of the pseudomonas B5 is indirect algae dissolving, the algae dissolving activity of the sterile filtrate is similar to that of the original bacterial liquid, the sterile filtrate treated at high temperature still has higher algae dissolving effect, and the pseudomonas B5 is presumed to exert algae dissolving effect through secretion of biological macromolecules and heat-resistant micromolecules.
Drawings
FIG. 1 shows the color contrast of the algae solution after 14 days of action of Pseudomonas B5 cultured in 1/4R2A medium, wherein CK1, CK2, T0, T1, T2 and T3 are sequentially arranged from left to right.
FIG. 2 shows chlorophyll a content of the algae liquid after treatment of Pseudomonas B5 cultured in 1/4R2A medium.
FIG. 3 shows the algicidal efficiency after treatment with Pseudomonas B5 in 1/4R2A medium.
FIG. 4 shows the color contrast of the algae solution after 14 days of action of Pseudomonas B5 cultured in the novel medium, wherein CK1, CK2, T0, T1, T2 and T3 are sequentially arranged from left to right.
FIG. 5 shows chlorophyll a content of the algae solution after treatment with Pseudomonas B5 cultured in the novel medium.
FIG. 6 shows the algicidal effect of the novel medium-cultured Pseudomonas B5 treatment.
Detailed Description
The experimental methods used in the following examples are conventional methods unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The invention provides a novel culture method and application of pseudomonas B5.
The novel culture method and the application of the pseudomonas B5 provided by the invention are as follows: pseudomonas B5 is cultured by adopting a novel culture medium, and the application of the Pseudomonas B5 cultured by adopting the novel culture medium in algae removal is provided.
Pseudomonas sp.B5, 2019, the subject group was isolated from soil samples of the North Svalbard island,
wherein the novel culture medium consists of the following components: NH (NH) 4 H 2 PO 4 1g，MgSO 4 ·7H 2 O0.2 g, trisodium citrate 0.5g, K 2 HPO 4 1g, add dH 2 O was constant to 1,000mL.
The cultivation operation is as follows: taking out Pseudomonas sp.B5 preserved at-20deg.C, streaking 10 μl of bacterial liquid on LB solid medium, culturing in a constant temperature incubator for 24 hr, picking single colony in 60mL of the new culture medium, and culturing for 24 hr.
The algae dissolving mode is indirect algae dissolving.
The invention takes pseudomonas B5 from North Svalbard island as an experimental material, and determines the quantity of algae cells and the OD of algae liquid 720 And chlorophyll a content, the pseudomonas B5 has obvious algicidal effect on the microcystis aeruginosa. After the pseudomonas B5 cultured by the novel culture medium is treated for 14 days, the algicidal rate reaches 87%.
1.1 Experimental materials
1.1.1 test algae species and culture
The algae used in this experiment was microcystis aeruginosa (Microcystis aeruginosa) purchased from the institute of aquatic organisms in the national academy of sciences (Hubei). The culture medium used for the algae strain is BG11. The algal cells were used for experiments after culturing in a light incubator to the logarithmic growth phase. The culture condition is 25 ℃, and the light-dark period ratio of the illumination intensity of 1000-2000lx is 12h to 12h.
1.1.2 species
The strain is Pseudomonas sp.B5 which is isolated from soil samples of North Svalbard islands in the laboratory.
The 16S rDNA of the B5 strain was PCR amplified and sequenced and compared with the database of EzTaxon model strains, and identified as Pseudomonas (Pseudomonas) strain. The strain is submitted to China general microbiological culture collection center (CGMCC) for patent preservation, and verified and identified that B5 is Pseudomonas strain with a preservation number of 22780.
The method comprises the following steps: pseudomonas sp.B5 16S rDNA sequence
GTCGAGCGGTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTTCGGAAACGGACGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCTACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTTACCTAATACGTGATTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTAATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGAAGCCTTGAGCTTTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTAATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAGAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCACAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTACCACGG
Classification naming of biological materials: pseudomonas sp.
Strain number of biological material: B5B 5
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection of microorganisms)
The preservation unit of biological materials is abbreviated as: CGMCC
Deposit unit address of biological material: beijing city, the North Chen Xili No. 1, 3 national academy of sciences of China for microbiology
Preservation date of biological material: 2021.6.24
Accession numbers of the collection center of biological materials: 22780
1.1.3 major reagents
BG11 medium: BG 11.7 g was dissolved in 1L of sterile water. Is used for culturing microcystis aeruginosa.
LB medium: 10g of tryptone, 5g of yeast extract, 10g of NaCl, and adding 15g of agar into a solid culture medium until the volume reaches 1L. Is used for activating algicidal bacteria.
1/4R2A Medium: 0.25g of tryptone, 0.5g of acid hydrolyzed casein, 0.5g of yeast extract powder, 0.5g of soluble starch, 0.3g of dipotassium hydrogen phosphate, 0.1g of magnesium sulfate, 0.3g of sodium pyruvate, 0.25g of peptone, 0.5g of glucose and pH value of 7.2+/-0.2 and 25 ℃. 0.8g of the product is weighed and dissolved in 1000mL of distilled water by heating. Is used for culturing algicidal bacteria and verifying algicidal mode.
New culture medium [ Yujian, wang Pingyu, wanglong, she Wenjing, xinhua, liu Mingchuan. Research on method for separating and identifying algicidal bacteria and evaluating algicidal effect [ J ]]Chemical engineering, jiangxi, 2011,69 (2) 20-22]：NH 4 H 2 PO 4 1g，MgSO 4 ·7H 2 O0.2 g, trisodium citrate 0.5g, K 2 HPO 4 1g, add dH 2 O was constant to 1,000mL. For use inCulturing algicidal bacteria and verifying algicidal mode.
1.1.4 Main instruments
Ultra-clean bench, sterilizing pot, constant temperature incubator, illumination incubator, visible spectrophotometer, blood cell counting plate, optical microscope, table centrifuge, shaker, water bath pot, adjustable pipettor, etc.
1.2 Experimental methods
1.2.1 cultivation of algae solution and determination of algae solution concentration
Transferring the algae liquid into BG11 culture medium at ratio of 1:10, and culturing in illumination incubator for several days until the algae liquid concentration reaches 10 7 Individual/mL (corresponding to OD 720 ≈0.5)。
1.2.2 cultivation of bacterial liquid
Taking out Pseudomonas strain preserved at-20deg.C, streaking 10 μl of bacterial liquid on LB solid plate, culturing in constant temperature incubator for 24 hr, picking single colony in 20mL new culture medium, and culturing under optimal condition for 24 hr.
1.2.3 exploration of the algicidal Activity and algicidal Property of Pseudomonas B5
Reference to Chen Liting et al [ Chen Liting, levojun, song Lirong, liu Jin, gannan Qin. Algicidal bacterial screening and Effect of algicidal active substances on the physiological Activity of Microcystis aeruginosa [ J ]]Aquatic biologicals, 2020,44 (3): 638-646]The bacterial liquid was centrifuged at 5000 rpm at 4℃for 10min, and the supernatant was collected. The bacterial cells in the filtrate were filtered off with a 0.22 μm filter, and the filtrate was divided into two portions, one portion was stored at 4℃for (T1) and the other portion was treated at 121℃for 15 minutes at high temperature (T2). The bacterial pellet was washed 2 times with physiological saline and resuspended in an equal volume of physiological saline and stored at 4 ℃ (T3). Mixing 3mL of stock solution (T0), T1, T2, T3, physiological saline (CK 1), bacterial culture medium (CK 2) and 12mL of microcystis aeruginosa solution uniformly, culturing in an illumination incubator for 14 days, observing the color of each group of solution, counting the number of microcystis cells, and determining the OD of the solution 720 And judging the algae dissolving mode by the value and the chlorophyll a content of the algae liquid.
1.2.4 determination of chlorophyll a content of algae liquid
The extraction method of chlorophyll a adopts a hot ethanol extraction method [ Chen Yu, chen Kaining, hu Yaohui ], a "hot ethanol method" for measuring chlorophyll a of phytoplankton and measuring errors is discussed [ J ]. Lake science, 2006, (05): 550-552], and the supernatant is discarded after 4850g of algae solution treated by algicidal bacteria is centrifuged at 4 ℃ for 20min, and sediment is frozen at-20 ℃ for more than 24h. Taking out the centrifuge tube filled with the precipitate, adding a preheated 90% ethanol solution into the centrifuge tube, carrying out water bath at 85 ℃ for 2min, extracting the sample at room temperature and in a dark place for 4-6h, centrifuging at 4850g and 4 ℃ for 10min, and collecting the supernatant to obtain chlorophyll a extract.
Measuring absorbance values of chlorophyll a extract at 665nm and 750nm respectively with 90% ethanol as reference, adding 1 drop of 0.1M hydrochloric acid into the cuvette, mixing, measuring absorbance values of acidified extract at 665nm and 750nm again after 1min, and calculating chlorophyll a content.
2 experimental results and discussion
(1) Algicidal effect of 1/4R2A culture medium for culturing pseudomonas B5 bacterial liquid
Pseudomonas is cultured by using a 1/4R2A culture medium, the color of the algae liquid after the pseudomonas is treated by the microcystis aeruginosa for 14 days is observed (figure 1), the chlorophyll a content is determined, and the algae killing rate is calculated. The algae killing rate of the original bacterial liquid (T0) is 66.03%, the algae killing rate of the sterile filtrate (T1) is 64.14%, the algae killing rate of the filtrate (T2) treated at high temperature is 66.47%, and the bacteria killing rate is not obviously different from that of T0. Compared with the filtrate treatment groups T1 and T2, the cell group (T3) has a lower algicidal effect. In addition, the 1/4R2A culture medium has obvious promotion effect on the growth of microcystis aeruginosa, so that the algae cell number of the algae liquid is greatly increased, and the algae liquid is dark green (Table 1, figures 2-3).
Table 1 1/4R2A cultured B5 treatment for 14 days was followed by algicidal effect on Microcystis aeruginosa
(2) Novel algae dissolving effect of pseudomonas B5 bacterial liquid cultured by culture medium
Pseudomonas B5 was cultured using a novel medium, the color of the algae solution after the Pseudomonas treatment of Microcystis aeruginosa for 14 days was observed (FIG. 4), the chlorophyll a content was determined and the algicidal rate was calculated. Compared with the Pseudomonas B5 cultured by the 1/4R2A culture medium, the algicidal rate of the Pseudomonas stock solution (T0) cultured by the novel culture medium is improved to 87.45 percent. The sterile filtrate (T1) has similar algicidal effect as the original bacterial solution (T0). The algicidal effect of the high temperature treated filtrate (T2) was reduced to 64.74% but still had a high algicidal activity. The algicidal effect of cell group T3 was lower than that of filtrate treatment groups T1, T2 (Table 2, FIGS. 5-6).
The sterile filtrate T1 has similar algae dissolving effect with the original bacterial solution (T0), which shows that the algae dissolving mode of the pseudomonas B5 is indirect algae dissolving, and the B5 is presumed to have the killing effect on the microcystis aeruginosa by secreting algae dissolving substances. The sterile filtrate (T2) treated at high temperature still has a certain algae dissolving effect, which shows that the algae dissolving effect of B5 can be the result of the combined action of bioactive macromolecules (enzymes) and heat-resistant inorganic small molecules.
TABLE 2 algicidal Rate of microcystis aeruginosa after 14 days of novel Medium-cultured B5 treatment
In conclusion, the pseudomonas B5 cultivated by the novel culture medium has higher algicidal activity, and the algicidal rate reaches about 87% after 14 days of treatment. The algae killing rate of the sterile filtrate is the same as that of the original bacterial liquid, and the sterile filtrate after high-temperature treatment still keeps higher algae dissolving activity, which shows that the algae dissolving mode of the pseudomonas B5 is mainly indirect algae dissolving, and the algae dissolving effect is probably the result of the combined action of bioactive macromolecules secreted by the pseudomonas B5 and heat-resistant inorganic micromolecules.
SEQUENCE LISTING
<110> university of capital and education
<120> application of Pseudomonas B5 in algae removal
<130> GNCAQ211638
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1405
<212> DNA
<213> Pseudomonas sp.
<400> 1
gtcgagcggt agagagaagc ttgcttctct tgagagcggc ggacgggtga gtaatgccta 60
ggaatctgcc tggtagtggg ggataacgtt cggaaacgga cgctaatacc gcatacgtcc 120
tacgggagaa agcaggggac cttcgggcct tgcgctatca gatgagccta ggtcggatta 180
gctagttggt gaggtaatgg ctcaccaagg ctacgatccg taactggtct gagaggatga 240
tcagtcacac tggaactgag acacggtcca gactcctacg ggaggcagca gtggggaata 300
ttggacaatg ggcgaaagcc tgatccagcc atgccgcgtg tgtgaagaag gtcttcggat 360
tgtaaagcac tttaagttgg gaggaagggc agttacctaa tacgtgattg ttttgacgtt 420
accgacagaa taagcaccgg ctaactctgt gccagcagcc gcggtaatac agagggtgca 480
agcgttaatc ggaattactg ggcgtaaagc gcgcgtaggt ggtttgttaa gttggatgtg 540
aaatccccgg gctcaacctg ggaactgcat tcaaaactga ctgactagag tatggtagag 600
ggtggtggaa tttcctgtgt agcggtgaaa tgcgtagata taggaaggaa caccagtggc 660
gaaggcgacc acctggacta atactgacac tgaggtgcga aagcgtgggg agcaaacagg 720
attagatacc ctggtagtcc acgccgtaaa cgatgtcaac tagccgttgg aagccttgag 780
cttttagtgg cgcagctaac gcattaagtt gaccgcctgg ggagtacggc cgcaaggtta 840
aaactcaaat gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag 900
caacgcgaag aaccttacca ggccttgaca tccaatgaac tttctagaga tagattggtg 960
ccttcgggaa cattgagaca ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt 1020
gggttaagtc ccgtaacgag cgcaaccctt gtccttagtt accagcacgt aatggtgggc 1080
actctaagga gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aagtcatcat 1140
ggcccttacg gcctgggcta cacacgtgct acaatggtcg gtacagaggg ttgccaagcc 1200
gcgaggtgga gctaatccca caaaaccgat cgtagtccgg atcgcagtct gcaactcgac 1260
tgcgtgaagt cggaatcgct agtaatcgcg aatcagaatg tcgcggtgaa tacgttcccg 1320
ggccttgtac acaccgcccg tcacaccatg ggagtgggtt gcaccagaag tagctagtct 1380
aaccttcggg aggacggtac cacgg 1405
Claims (3)
1. The application of the pseudomonas B5 cultured by the culture medium in algae removal;
pseudomonas sp.B5, deposited at 2021.6.24 with China general microbiological culture Collection center (CGMCC), number 22780;
the culture medium consists of the following components: NH (NH) 4 H 2 PO 4 1 g，MgSO 4 7H 2 O0.2 g, trisodium citrate 0.5g, K 2 HPO 4 1g, add dH 2 O constant volume to 1000 mL.
2. The use according to claim 1, characterized in that: the cultivation operation is as follows: taking out Pseudomonas sp.B5 stored at-20deg.C, streaking 10 μl of the bacterial liquid on LB solid medium, culturing 24h in a constant temperature incubator, picking single colony in 60mL medium, and culturing 24h.
3. The use according to claim 1, characterized in that: the algae removal mode is indirect algae dissolution.
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| CN103937726A (en) * | 2014-04-29 | 2014-07-23 | 吉林农业大学 | Alga-lysing pseudomonas aeruginosa and application thereof |
| CN104498387B (en) * | 2014-11-25 | 2017-06-16 | 辽宁大学 | A kind of pseudomonas aeruginosa and its application |
| EP3254565A1 (en) * | 2016-06-06 | 2017-12-13 | Etablissements J. Soufflet | Microbial strains for biologically controlling fusarium head blight |
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