CN113567405A - Paper-based microfluid diode device and visual biomolecule detection method - Google Patents
Paper-based microfluid diode device and visual biomolecule detection method Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
- B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
- B41J2/00—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed
- B41J2/005—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
- B41J2/01—Ink jet
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention relates to a paper-based microfluid diode device and a visual biomolecule detection method, wherein the diode device structurally comprises a forward dripping pool and a reverse dripping pool, and the forward dripping pool and the reverse dripping pool are connected through a micro-channel; the forward direction dropping liquid pool is provided with a first wall surface which is vertically connected with one end of the micro-channel; the reverse drip pool is provided with a second wall surface which is symmetrically arranged, the second wall surface is connected with the other end of the micro channel at an angle alpha, a fluid contact angle formed by liquid flowing from the forward drip pool to the reverse drip pool in the micro channel is theta, and the sum of alpha and theta is less than 90 degrees. The one-way conduction characteristic of the diode device is utilized, and the reverse drip pool is utilized to adsorb the upconversion nanometer fluorescent material with a certain concentration. During detection, the forward dripping pool is used for discharging a solution to be detected, the detected object is enriched through capillary action and transported to the reverse dripping pool, and fluorescence analysis is carried out through photographing, so that quantitative and accurate detection of biomolecules is realized.
Description
Technical Field
The invention relates to the technical field of microfluidic chips, in particular to a paper-based microfluidic diode device manufactured based on an ink-jet printing technology and a visual biomolecule detection method.
Background
The diode is one of the most basic elements in an electronic circuit, can realize unidirectional current conduction and plays an important role in miniaturization of electronic devices. For the fluid flow control system, the development of microminiaturization is also directed, and the main objective is to integrate operation modules in physical and biochemical reactions, analysis reactions, such as sampling, dilution, reaction, separation, detection, etc., on a chip of several microns or tens of microns for automation. And the key component to accomplish these operations is the fluid diode. On a macroscopic scale, the function of a fluid diode can be performed by a valve with moving parts. On a small scale, however, these moving parts can increase manufacturing costs and present problems of unreliability, requiring extremely high energy to start operation, and the like.
In the prior art, a microfluidic diode mainly aims at controlling ion transport current to achieve the current rectification effect. Control of the flow of the fluid itself is difficult due to the ordered control of the flow of the fluid, particularly the liquid sample required during the test, at the microscale. Therefore, the development of the microfluidic diode with a fixed structure to realize the directional flow of the fluid has important significance for the development of the microfluidic chip.
The micro-fluidic chip detection technology mainly comprises a colorimetric method, a fluorescence method, an electrochemical detection method and the like, wherein the fluorescence analysis method is a method for performing qualitative or quantitative analysis by utilizing fluorescence which is generated when some substances enter an excited state after being irradiated by light with specific wavelength and can reflect the characteristics of the substances when the substances are restored to a ground state from the excited state. However, the current fluorescence analysis method usually adopts ultraviolet light as an excitation light source, the penetration depth of ultraviolet light excitation light tissue is limited, deep tissue signal molecules cannot be effectively excited, and the interference of organism autofluorescence and the like can be caused, and meanwhile, a traditional fluorescence method needs a fluorescence photometer or a fluorescence spectrophotometer. These devices are often required to be used in laboratories, are not readily available and are not easily adjustable.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a paper-based microfluid diode device and a visual biomolecule detection method, aiming at realizing accurate control and accurate test of fluid flow.
The technical scheme adopted by the invention is as follows:
a paper-based microfluidic diode device comprises a forward dripping pool and a reverse dripping pool, wherein the forward dripping pool and the reverse dripping pool are connected through a microchannel; the forward direction dropping liquid pool is provided with a first wall surface which is vertically connected with one end of the micro-channel; the reverse drip pool is provided with a second wall surface which is symmetrically arranged, the second wall surface is connected with the other end of the micro channel at an angle alpha, a fluid contact angle formed by liquid flowing from the forward drip pool to the reverse drip pool in the micro channel is theta, and the sum of alpha and theta is less than 90 degrees.
As a further improvement of the above technical solution:
the diode means is formed by a hydrophobic border on the paper.
The hydrophobic boundary is a hydrophobic barrier left by the printer-ejected hydrophobic ink after drying.
And a luminescent material layer is arranged in the reverse dropping liquid pool.
The luminescent material layer is obtained by dripping up-conversion nano fluorescent material solution into the reverse dropping pool and drying.
The cross section of the micro flow channel is rectangular, and the width of the micro flow channel is 50 mu m.
The forward direction drip pool is a rectangular groove, and the width dimension of the forward direction drip pool is 10-30 mm.
A visual biomolecule detection method using a paper based microfluidic diode device formed by hydrophobic boundary printing on hydrophilic paper based on inkjet printing, comprising the steps of:
drop into reverse drip pond with up-conversion nanometer fluorescent material solution, solution is full of reverse drip pond and microchannel, but can not flow into forward drip pond:
after the up-conversion nano fluorescent material solution is dried, irradiating the reverse dripping pool by using a laser, taking a picture under a dark condition, and calculating the total fluorescence intensity of the picture;
adding a sample solution to be detected into the forward dropping liquid pool, and enabling the sample solution to flow into the reverse dropping liquid pool through a micro-channel;
and after the sample solution is dried, irradiating the reverse dripping pool by using a laser, taking a picture under a dark condition, calculating the change of the total fluorescence intensity of the two pictures, and comparing the change with a standard curve to obtain the concentration of the biomolecule.
The invention has the following beneficial effects:
the device realizes different flow mechanisms in the positive direction and the negative direction through the design of the effective contact angle so as to form different starting pressures and realize the unidirectional flow of fluid in a larger pressure range. The size of the pressure range can be varied by controlling the width dimension and wettability of the microchannel.
The device has simple structure and convenient material acquisition, utilizes the ink-jet printing technology and adopts paper materials in micro-scale compared with other similar manufacturing modes using the micro surface modification technology, has rich sources, can be produced in batches, is degradable, is portable and easy to operate, and greatly reduces the manufacturing difficulty and the cost. The high precision of the ink-jet printer determines the minimum width of the micro-flow channel, and the micron-level printing precision can increase the pressure range of the device to about several thousand Pa.
The biomolecule testing method provided by the invention adopts the microfluidic diode device, forward flow does not need a driving force, a passive spontaneous working mode enables fluid flow to be more reliable, the concentration of an up-conversion material in a detection area is ensured to be constant, enrichment of a detected target substance is realized through a paper base, and the detection precision and speed are greatly improved.
Drawings
FIG. 1 is a schematic diagram of the structure of one form of the apparatus of the present invention.
Fig. 2 is a schematic structural diagram of another form of the apparatus of the present invention.
FIG. 3 is a schematic diagram of the operation of the device of the present invention in a forward flow mode.
FIG. 4 is a schematic view of the working principle of the device of the present invention in which the direction of the device is not circulated.
FIG. 5 is a schematic structural diagram of a specific device used in the method for detecting biomolecules according to the present invention.
FIG. 6 shows the results of the biomolecule detection method according to an embodiment of the present invention.
In the figure: 1. a forward direction drip pool; 2. a micro flow channel; 3. a reverse drip tank; 4. a hydrophobic boundary; 11. a first wall surface; 31. and a second wall surface.
Detailed Description
The following describes embodiments of the present invention with reference to the drawings.
The paper-based microfluidic diode device of the present embodiment, as shown in fig. 1 and 2, includes a forward droplet pool 1 and a reverse droplet pool 3, and the forward droplet pool 1 and the reverse droplet pool 3 are connected by a microchannel 2; the forward direction dropping liquid pool 1 is provided with a first wall surface 11, and the first wall surface 11 is vertically connected with one end of the micro-channel 2; the reverse drip pool 3 has a second wall surface 31 symmetrically arranged, as shown in fig. 3, the second wall surface 31 is connected with the other end of the micro channel 2 at an angle α, a fluid contact angle formed by the liquid flowing from the forward drip pool 1 to the reverse drip pool 3 in the micro channel 2 is θ, and the sum of α and θ is less than 90 degrees.
In the above embodiment, the shape of the inverted drip chamber 3 may be specifically configured as a "drop-like" structure as shown in fig. 1, where the "drop-like" structure includes the wall surface two 31 and the arc-shaped edge smoothly connected to the wall surface two 31. The inverted drip chamber 3 is also shaped as a "trumpet" or "funnel" as shown in FIG. 2. Other similar structures can be adopted as long as the included angle between the second wall surface 31 and the micro flow channel 2 is alpha.
As can be seen from fig. 3, α is an acute angle formed between the central axis of the microchannel 2 (the direction of flow toward the droplet tank 3) and the second wall surface 31. In the above embodiment, the micro flow channel 2 has a rectangular cross section and a width (perpendicular to the flow direction) of several tens to several hundreds of micrometers. Preferably, 50 μm is taken.
In the above embodiment, the forward drip chamber 1 is preferably a rectangular tank, and the width dimension (i.e. the dimension along the direction perpendicular to the micro flow channel 2) is in mm, which is 10-30 mm. Preferably, 15mm is taken.
The paper-based microfluidic diode device of the above embodiment is formed by the hydrophobic boundary 4 on the hydrophilic paper, and the regions formed inside the hydrophobic boundary 4 are all hydrophilic regions. The paper is preferably hydrophilic filter paper.
Specifically, the hydrophobic boundary 4 is a hydrophobic barrier left by the printer-ejected hydrophobic ink after drying.
The hydrophobic ink may be a mixed ink of permanent marker ink and alcohol, and the mixed ink may be volatilized to leave the hydrophobic resin and colorant as a hydrophobic barrier.
In particular, the thickness of the hydrophobic boundary 4 is 1 mm.
The paper-based microfluidic diode device of the above example works:
the fluid in the forward drip pool 1 can spontaneously fill the micro-channel 2 and smoothly flow to the reverse drip pool 3, so that forward flowing conduction is realized; on the contrary, the fluid in the reverse droplet pool 3 permeates into the micro channel 2, and when the fluid flows through the end of the micro channel 2, the fluid cannot smoothly flow into the forward droplet pool 1 due to the surface tension of the fluid, that is, the reverse flow is not conducted.
The working principle of the paper-based microfluidic diode device of the above embodiment:
osmotic pressure refers to the minimum pressure required for fluid from the reservoir to enter and fill the capillary tube. Relief pressure refers to the minimum pressure required for fluid to flow from a channel of smaller cross-sectional size. The equation for the young laplace to calculate the minimum pressure required is as follows:
p＝(-2γcosθ)/H (1)
wherein gamma is the surface tension coefficient of the fluid, H is the width of the flow channel, and theta is the contact angle of the fluid;
the cross-sectional dimensions (width, height) of the micro flow channel 2 of the present embodiment are set to the order of micrometers in order to determine the pressure drop range of the unidirectional flow of the diode device due to the osmotic pressure and the release pressure of the micro flow channel 2. The smaller the cross-sectional size of the micro flow channel 2, the greater the fluid surface tension influence. When the width is reduced to a micron level, the difference between the osmotic pressure and the release pressure of the micro-channel 2 can reach megapascals, the pressure drop range for controlling the unidirectional flow of fluid is greatly improved, and the inner wall of the micro-channel 2 is a hydrophobic wall formed by hydrophobic ink. The width of the section of the micro flow channel 2 cannot be too large, and at least one atmosphere can be ensured to ensure that the fluid in the reverse drip pool 3 cannot spontaneously flow into the drip pool 1.
In operation, the activation pressure for the forward and reverse flows are different due to the different flow mechanisms. In the forward flow, the infiltration process through the micro flow channel 2 is required, which can be illustrated by referring to the principle of the forward conduction process in fig. 3. According to the formula (1), theta is less than 90 DEG, and the infiltration pressure P of the microchannel 2inLess than 0, and because the effective contact angle theta + alpha is less than 90 degrees, the fluid can spontaneously enter the reverse drip pool 3 along the edge of the micro-channel 2; on the other hand, when the fluid flows in the reverse direction, as shown in fig. 4, the fluid needs to penetrate into the micro flow channel 2, and then when the fluid reaches the end of the micro flow channel 2, α is 90 °, the contact angle of the fluid increases due to the obstruction of the surface tension of the fluid, and the effective contact angle θ increases*Above 90 deg., fluid flow stops. Only by further increasing the driving pressure can the constraint of the surface tension of the fluid be broken through, thus realizing reverse conduction. Therefore, the pressure for reverse conduction is much higher than that for forward conduction.
The device is made of paper based on an ink-jet printing technology, fluid is guided by hydrophilic cellulose or nitrocellulose, and is automatically conveyed to an expected outlet from an inlet of the micro-channel 2 through capillary action, and driving force is not needed in forward working. At the micro scale, the osmotic pressure and the release pressure of the fluid in the micro flow channel 2 are greatly different, so that the unidirectional flow of the fluid in a larger pressure range can be realized.
At present, the printing precision of most household printers also reaches the micron level, and ink droplet size of ink jet printer can reach 1.5 picoliters even, and this embodiment uses the mixture of marker ink and alcohol to make the black and replace the former dress black of printer and print, specifically adopts piezoelectric ink jet printer to carry out the production of this embodiment diode device, and specific operating procedure is as follows:
i. prying a new ink box in the printer, taking out the sponge and the filter block, cleaning with clear water, cleaning once with the ink cleaning agent, and drying after cleaning with the clear water. And prevents water or cleaning agents from contacting the chip.
And ii, splicing the cleaned and dried ink box as it is, and sticking the pried ink box to be completely sealed by using hot melt adhesive.
Configuring the marker ink, wherein the volume ratio of the marker ink to the ethanol is 1: 5. Filtering the marker ink through a 0.45-micron filter membrane, adding 5 times of ethanol, and placing the mixture into a beaker to be uniformly stirred.
And iv, sucking the configured ink by using a syringe and injecting the ink into the ink box, wherein the injection speed is slow so as to avoid generating bubbles to overflow the ink box. After the injection was completed, the injection port was closed with an adhesive tape.
v. replacing the original printer cartridge with the modified cartridge.
Printer self-cleaning. A paper feeding device is set aside at a paper feeding port of the printer, A4 paper is loaded, and the pattern of the nozzle check is printed according to the screen prompt and compared.
Drawing the diode pattern with drawing software (AutoCAD).
Replace Whatman Gradel filter paper (or Xinhua No. 1 filter paper, cut to A4 size in advance), print the paper-based micro flow channel on both sides multiple times. The printed filter paper was air-dried for 15 minutes to evaporate the ethanol. The hydrophobic resin and colorant remain in the filter paper forming a visible hydrophobic barrier.
In view of the fact that the forward and reverse dropping wells of the diode device of the above embodiment are suitable for being used as the sample application region and the detection region, respectively, the following provides a detection method for visualizing biomolecules by using the paper-based microfluidic diode device of the above embodiment, comprising the following processes:
dripping the up-conversion nano fluorescent material solution into the reverse dripping pool 3, wherein the solution can fill the reverse dripping pool 3 and the micro channel 2;
after the up-conversion nano fluorescent material solution is dried, irradiating the reverse dropping pool 3 by using a laser, taking a picture under a dark condition, and calculating the total fluorescence intensity of the picture;
adding a sample solution to be detected into the forward dripping pool 1, and enabling the sample solution to smoothly flow into the reverse dripping pool 3;
and after the sample solution is dried, irradiating the reverse dropping pool 3 by using a laser, taking a picture under a dark condition, calculating the change of the total fluorescence intensity of the two pictures, and comparing the change with a standard curve to obtain the concentration of the biomolecule.
Referring to fig. 5, a specific test device and a specific test process are shown, in which the "paper-based microfluidic chip" refers to the paper-based microfluidic diode device of the above embodiment. Near infrared light of 808nm or 980nm can be used as an excitation light source of the laser; and taking a picture of the laser irradiation position by adopting the mobile phone loaded with the optical filter under a dark condition. The filter can adopt a 700nm short-pass filter. The fluorescence intensity can be rapidly calculated by adopting professional software such as ImageJ and the like. The concentration of the biomolecule in the patch solution can be calculated from the efficiency of quenching (the difference between the two total fluorescence intensities divided by the total fluorescence intensity of the untreated sample).
The up-conversion nano fluorescent material can adopt the following materials: NaGdF 4: yb, Nd, Er, NaGdF 4: er, NaGdF 4: yb, Er @ NaYbF 4: nd, NaYF 4: yb, Er, Nd @ NaYF 4: nd, and the like. The biomolecule in the sample solution has absorption peak at 550nm, and comprises adriamycin, epiadriamycin, mitoxantrone, daunomycin, demethyldaunomycin, anthocyanin, and dopamine. The upconversion nanometer fluorescent material emitting 550nm visible light is used as a fluorescent probe, and biomolecules such as dopamine and adriamycin can quantitatively quench the upconversion nanometer luminescent fluorescent probe, so that rapid, sensitive and visual detection of biomolecules such as dopamine and adriamycin is achieved.
In the detection method, the forward dripping pool 1 and the reverse dripping pool 3 are respectively used as a sample adding area and a detection area, the upconversion nano fluorescent material solution is dripped into the reverse dripping pool 3, the solution is filled in the reverse dripping pool 3 and the micro channel 2, but the upconversion nano fluorescent material solution cannot flow into the forward dripping pool 1 due to the rectification working mechanism of the diode device. The excellent rectification characteristic of the paper-based diode device is utilized to realize the visual quantitative detection of the biomolecules by the fluorescence method, and the following important problems can be solved:
firstly, the up-conversion nano fluorescent material solution can be quantitatively dripped into the reverse dripping pool 3 and expanded in the regionBut the sample can not flow into the forward direction dropping liquid pool 1 area all the time, thus effectively solving the self-separation of the detection area and the sample adding area. Secondly, after the upconversion nano-phosphor solution is dried, the sample solution is added into the positive direction dropping well 1 due to the negative pressure (infiltration pressure P) mentioned abovein< 0), the sample solution can automatically flow to the detection area, namely the reverse drip pool 3, along the micro-channel 2, and diffuse in the detection area 3, and fully mix with the up-conversion material and quench, and the sample solution to be detected can be enriched.
The paper-based microfluidic diode device of the above embodiment can be produced efficiently in large batches by means of inkjet printing:
a layer of luminescent material is provided within the inverted drip chamber 3. The luminescent material layer can be obtained by dripping up-conversion nano fluorescent material solution into the reverse dripping pool 3 in advance and drying.
Equal and quantitative up-conversion material solution is added in advance to the region in reverse drip chamber 3, and in laboratory experiment or field in-situ test, can directly take, need not the professional, can use like the test paper, and at the sample application district (forward drip chamber 1) dropwise add the sample that awaits measuring, send to the high in the clouds after shooing and calculate fluorescence value and obtain biomolecule concentration ratio.
The following is a further description of the detection method for visualizing biomolecules with specific embodiments:
the total fluorescence intensity change of the doxorubicin solutions (sample solutions) at different concentrations of 100. mu.M and 200. mu.M, respectively, was detected.
The parameters of the mobile phone camera are set as follows: 1.85 (focal length), 1000 (sensitivity), 1/5 (shutter), the mobile phone model is millet 11.
And (3) dropwise adding 1 mu L of NaYF4 with the concentration of 1mg/mL into the reverse dropping pool 3: yb, Er, Nd @ NaYF 4: the Nd up-conversion material solution is used after being dried;
irradiating the reverse drip pool 3 by using a 808nm laser, and adjusting the laser coverage angle to take 3 times of pictures;
dripping 1 μ L of sample solution (100 μ M and 200 μ M of adriamycin solution) into the forward direction dripping pool 1, drying, and taking 3 times of pictures;
the taken picture was implanted into ImageJ for total fluorescence intensity calculation.
The average value of the total fluorescence intensity of the three tests and the quenching efficiency are compared as shown in FIG. 6, and the total fluorescence quenching efficiency and the adriamycin concentration have a linear relation and a linear range. With the dropwise addition of the concentration of the adriamycin solution, the quenching of the up-conversion nano fluorescent material is increased, the total fluorescence intensity is reduced immediately, the quenching efficiency is increased, and the quenching efficiency in the experiment is divided into 17.738% and 23.697%.
Claims (8)
1. The paper-based microfluidic diode device is characterized by comprising a forward dripping pool (1) and a reverse dripping pool (3), wherein the forward dripping pool (1) and the reverse dripping pool (3) are connected through a microchannel (2); the forward direction dropping liquid pool (1) is provided with a first wall surface (11), and the first wall surface (11) is vertically connected with one end of the micro flow channel (2); the reverse dripping pool (3) is provided with a wall surface two (31) which is symmetrically arranged, the wall surface two (31) is connected with the other end of the micro channel (2) at an angle alpha, a fluid contact angle formed by liquid flowing from the forward dripping pool (1) to the reverse dripping pool (3) in the micro channel (2) is theta, and the sum of the angle alpha and the angle theta is less than 90 degrees.
2. The paper-based microfluidic diode device according to claim 1, characterized in that the diode device is formed by a hydrophobic border (4) on a hydrophilic paper.
3. The paper-based microfluidic diode device according to claim 2, wherein the hydrophobic boundary (4) is a hydrophobic barrier left by drying of hydrophobic ink ejected by an inkjet printer.
4. The paper based microfluidic diode device according to claim 2, wherein a luminescent material layer is provided within the reverse drip reservoir (3).
5. The paper-based microfluidic diode device according to claim 4, wherein the luminescent material layer is obtained by dropping an upconversion nanofluorescent material solution into the reverse dropping tank (3) and drying.
6. The paper-based microfluidic diode device according to claim 1, characterized in that the micro flow channel (2) is rectangular in cross section with a width of 50 μm.
7. The paper based microfluidic diode device according to claim 1, wherein the forward drip reservoir (1) is a rectangular slot with a width dimension of 10-30 mm.
8. A visual biomolecule detection method using the paper based microfluidic diode device of claim 1, characterized in that the diode device is formed by printing of hydrophobic borders (4) on hydrophilic paper based on inkjet printing, the detection method comprising the steps of:
dripping the up-conversion nano fluorescent material solution into a reverse dripping pool (3), wherein the solution is filled in the reverse dripping pool (3) and the micro channel (2) but cannot flow into the forward dripping pool (1);
after the up-conversion nano fluorescent material solution is dried, irradiating the reverse dripping pool (3) by using a laser, taking a picture under a dark condition, and calculating the total fluorescence intensity of the picture;
adding a sample solution to be detected into the forward dripping pool (1), and enabling the sample solution to flow into the reverse dripping pool (3) through the micro-channel (2):
and after the sample solution is dried, irradiating the reverse drip pool (3) by using a laser, taking a picture under a dark condition, calculating the change of the total fluorescence intensity of the two pictures, and comparing the change with a standard curve to obtain the biomolecule concentration of the sample solution.
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| CN115779817A (en) * | 2022-12-06 | 2023-03-14 | 浙江大学 | Super-hydrophobic three-dimensional surface structure for liquid directional transportation and application |
| CN115779817B (en) * | 2022-12-06 | 2023-09-26 | 浙江大学 | Super-hydrophobic three-dimensional surface structure for directional liquid transportation and application |
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| CN113567405B (en) | 2022-11-04 |
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