CN113567213A

CN113567213A - Heating equipment for experiment container and biological sample preparation device

Info

Publication number: CN113567213A
Application number: CN202110927914.2A
Authority: CN
Inventors: 刘南峰; 阳方义; 潘雅楠
Original assignee: Rwd Life Science Co ltd
Current assignee: Rwd Life Science Co ltd
Priority date: 2021-08-13
Filing date: 2021-08-13
Publication date: 2021-10-29

Abstract

The embodiment of the invention provides heating equipment, which comprises a clamp-shaped device, a clamping device and a heating device, wherein the inner surface of the clamp-shaped device is matched with the outer surface of an experiment container and is used for clamping the experiment container or releasing the experiment container; at least one heating circuit disposed on an inner surface of the fixture-like device; wherein the clamp-like device comprises a body, a rotating shaft, a first arm and a second arm; the body forms a cavity around the experimental container; the rotating shaft is arranged on the body; the first arm and the second arm maintain or expand the body with the rotating shaft as a fulcrum. The inner surface of the clamp-shaped device provided by the embodiment of the invention is provided with the heating loop, so that the experiment container can be heated when the clamp-shaped device clamps the experiment container, the body of the clamp-shaped device forms a cavity surrounding the experiment container, the first arm and the second arm of the clamp-shaped device use the rotating shaft arranged on the body as a fulcrum, the body of the clamp-shaped device is unfolded or maintained by utilizing the lever principle, the loading and the dismounting are easy, and other operations in the experiment container cannot be influenced.

Description

Heating equipment for experiment container and biological sample preparation device

Technical Field

The invention relates to the technical field of laboratory instruments, in particular to heating equipment for an experimental container and a biological sample preparation device.

Background

In many biological experiments, it is often necessary to simulate the body temperature of an organism or to maintain a specific temperature during the preparation of a biological sample in order to facilitate the performance of the biological experiment. Therefore, there is a need for a heating device that can heat individual assay containers without affecting other operations of the biological assay while heating. For example, in preparing single cell suspensions, the operations of cutting, milling tissue, etc. are performed in the test vessels, while the biological sample in each test vessel needs to be maintained at a different specific temperature.

Disclosure of Invention

The embodiment of the invention provides heating equipment for an experimental container, and aims to solve the problem that different experimental containers are independently heated in the prior art.

In a first aspect, there is provided a heating apparatus for an assay vessel, comprising:

the inner surface of the clamp-shaped device (1) is matched with the outer surface of the experiment container and used for clamping the experiment container or releasing the experiment container;

at least one heating circuit (2), the heating circuit (2) being arranged on the inner surface of the clamp-like device (1);

wherein the clamp-like device (1) comprises a body (11), a rotating shaft (12), a first arm (13) and a second arm (14); the body (11) forms a cavity surrounding the experimental container; the rotating shaft (12) is arranged on the body (11); the first arm (13) and the second arm (14) maintain or expand the body (11) with the rotating shaft (12) as a fulcrum.

In a second aspect, there is provided a biological sample preparation device comprising a housing, a plurality of motors, a plurality of assay containers and a plurality of heating apparatus as described above;

a plurality of motors set up in the shell, and the drive shaft of a plurality of motors is connected with a plurality of experiment containers respectively, and a plurality of experiment containers of drive are rotatory, and a plurality of firing equipment centre gripping a plurality of experiment containers respectively heats.

The inner surface of the clamp-shaped device provided by the embodiment of the invention is provided with the heating loop, so that the experiment container can be heated when the clamp-shaped device clamps the experiment container, the body of the clamp-shaped device forms a cavity surrounding the experiment container, the first arm and the second arm of the clamp-shaped device use the rotating shaft arranged on the body as a fulcrum, the body of the clamp-shaped device is unfolded or maintained by utilizing the lever principle, the loading and the dismounting are easy, and other operations in the experiment container cannot be influenced.

Drawings

The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic view of a heating apparatus provided in accordance with an embodiment of the present invention;

FIG. 2 is a schematic diagram of an experimental container according to a first embodiment of the present invention;

FIG. 3 is a bottom view of a heating apparatus according to an embodiment of the present invention;

FIG. 4a is a schematic view of a clamp-like device 1 in a holding state according to an embodiment of the present invention;

FIG. 4b is a schematic view of the clamp-like device 1 in a spread-apart state according to an embodiment of the present invention;

FIG. 5 is a schematic view of a biological sample preparation device according to a second embodiment of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar modules or modules having the same or similar functionality throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.

Tissue is the cellular architecture that borders between cells and organs, and is composed of many morphologically similar cells and intercellular substances. For the cellular study of organisms, tissues need to be processed to prepare single cell suspensions. A single cell suspension is a biological sample that releases discrete individual cells as the connections between cells in the tissue are broken. The prepared single cell suspension has the characteristics of sufficient cell dispersion, high activity and the like, namely, the connection between cells needs to be fully destroyed, and the integrity and the activity of a single cell are kept, so that the single cell suspension is the basis for cell research. During the process of breaking the connections between cells in the tissue in the test vessel, the biological sample in the test vessel needs to be maintained at a specific temperature, such as 37 degrees celsius.

Example one

Fig. 1 is a schematic view of a heating apparatus according to an embodiment of the present invention. FIG. 2 is a schematic diagram of an experimental container according to a first embodiment of the present invention. Fig. 3 is a bottom view of a heating apparatus according to an embodiment of the present invention. With reference to fig. 1, 2 and 3, the heating apparatus comprises a clamp-like device 1 and at least one heating circuit 2.

The experimental container for preparing the single cell suspension is similar to a cylindrical test tube and consists of an experimental container body and a cover. The experimental container body and the cover are connected together through screw threads. The clamp-like device 1 forms a cavity around the test vessel, the inner surface of which matches the outer surface of the test vessel. The experimental container is put into a cavity formed by the clamp-shaped device 1, the size of the cavity is adjusted, and the clamp-shaped device 1 can clamp the experimental container and release the experimental container. At least one heating circuit 2 is a resistance heating circuit, and is arranged on the inner surface of the clamp-shaped device 1, and when the clamp-shaped device 1 clamps the experimental container, the heating circuit 2 is tightly attached to the outer surface of the experimental container, so that the experimental container can be heated. The heating circuit 2 may be battery powered or powered by an external power source. The heating circuit 2 extends at the inner surface of the clamp-like device 1 to the bottom of the clamp-like device 1 where contacts are formed for connection to an external power source.

In an embodiment of the invention, the clamp-like device 1 comprises a body 11, a rotation shaft 12, a first arm 13, a second arm 14 and a flap 15.

The body 11 forms a cavity around the test vessel, and the inner surface of the body 11, i.e. the inner surface of the clamp-like device 1, may match the shape of the outer surface of the test vessel. When the experimental container is a test tube similar to a cylinder, the cross section of the body 11 is a circular ring, and the circular ring extends for a certain length in the vertical direction; or the cross section of the body 11 is two semicircular rings, and the two semicircular rings extend in the vertical direction by a certain length. In order to clamp and release the experimental container, the size of the cavity formed by the body 11 can be adjusted, and as an embodiment of the invention, when the cross section of the body 11 is a circular ring, a gap, also called an opening, exists in the body 11 in the vertical direction; as another embodiment of the present invention, when the cross section of the body 11 is two semicircular rings, a gap, also called an opening, exists at one adjacent position of the two semicircular rings, and the other adjacent position is connected by a movable structure. The structure consisting of the rotating shaft 12, the first arm 13 and the second arm 14 distracts and maintains the body 11 so that the size of the cavity formed by the body 11 is adjustable. Maintaining the body 11 means that the body 11 is in a non-expanded, non-deformed state. When the experiment container needs to be heated, the body 11 is opened, the experiment container is loaded and then loosened, and heating is carried out; when the experiment container is not required to be heated, the body 11 is opened, and the experiment container is detached.

Fig. 4a is a schematic view of the clamp-shaped device 1 in a holding state according to an embodiment of the present invention. Fig. 4b is a schematic view of the clamp-like device 1 in a spread state according to an embodiment of the present invention. In the embodiment of the present invention, the rotating shaft 12 is provided on the body 11, penetrates the inner surface and the outer surface of the body 11, and the first arm 13 and the second arm 14 support or hold the body 11 with the rotating shaft 12 as a fulcrum. When the cross section of body 11 is the ring or two semicircle rings, axis of rotation 12 sets up in the predetermined position that is close to body 11 open-ended, and first arm 13 and second arm 14 are connected with axis of rotation 12 respectively, and first arm 13 extends body 11 top from axis of rotation 12, and second arm 14 extends to body 11 opening from axis of rotation 12, and first arm 13 and second arm 14 use axis of rotation 12 to form the lever as the fulcrum. An operator applies force to the first arm 13, the first arm 13 drives the second arm 14 to rotate in the same direction when rotating in one direction, and the second arm 14 extends out of the opening of the body 11 to expand the opening of the body 11, so that the cavity formed by the body 11 is enlarged, and the experimental container is released. When the first arm 13 rotates in the opposite direction, the second arm 14 is driven to rotate in the opposite direction, the second arm 14 is retracted at the opening of the body 11, the opening of the body 11 is reduced, the body 11 is restored, the formed cavity is reduced, and the experimental container is clamped. As another embodiment of the present invention, the rotational shaft 12 is accommodated between the inner surface and the outer surface of the body 11.

In the above embodiment, the rotating shaft 12 is provided on the body 11, and the position of the rotating shaft 12 in the vertical direction of the body 11 is not limited. The position of the rotating shaft 12 is a fulcrum of the lever, and the end of the second arm 14 far away from the rotating shaft 12 is a prying point. This sled is moved some and can be set up in 11 open-ended middle parts of body or bottom for body 11's whole deformation is comparatively even, is favorable to increase of service life, has reduced the requirement to making moreover.

In the present embodiment, it is preferable that the length of the first arm 13 is greater than the length of the second arm 14, and the operator can use a smaller force to the first arm 13. A shutter 15 is provided at the top of the body 11, in a preset position close to the first arm 13. When operating personnel exert oneself to first arm 13, baffle 15 carries on spacingly to first arm 13, avoids operating personnel to cross the operation and lead to damaging. Further, the heating device also comprises an elastic sleeve 3 sleeved at the bottom of the body 11, and resistance is formed for opening the body 11. By choosing a resilient sleeve 3 of suitable modulus of elasticity, the operator can apply comfortable forces to the first arm 13.

In an embodiment of the invention, the clamp-like device 1 further comprises a support 16 and a connector 17 arranged at the top of the body 11. The position of the support 16 does not coincide with the opening of the body 11 to enhance the rigidity of the body 11. The connecting member 17 is used for connecting the baffle 15 and the supporting member 16, and can enhance the rigidity of the body 11 and play a role of decoration.

Example two

FIG. 5 is a schematic view of a biological sample preparation device according to a second embodiment of the present invention. As shown in fig. 5, the biological sample preparation device comprises a housing, a plurality of motors, a plurality of experimental containers for preparing single cell suspensions, and a plurality of heating apparatuses described in the first embodiment. In the embodiment of the present invention, the structure of the heating apparatus is the same as that of the first embodiment, and includes all the features described in the first embodiment, which are not described herein again.

In the embodiment of the invention, a plurality of motors are arranged in the shell, the driving shafts of the motors are respectively connected with a plurality of experimental containers to drive the experimental containers to rotate, and meanwhile, a plurality of heating devices respectively clamp the experimental containers for heating. The biological sample preparation device also comprises a controller, wherein the controller respectively controls the plurality of heating devices, and the biological samples in the plurality of experimental containers can be maintained at different specific temperatures.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. Heating device for laboratory vessels, characterized in that it comprises:

a clamp-like device (1), the inner surface of the clamp-like device (1) is matched with the outer surface of an experiment container, and the clamp-like device is used for clamping the experiment container or releasing the experiment container;

at least one heating circuit (2), said heating circuit (2) being arranged on the inner surface of said clamp-like device (1);

wherein the clamp-like device (1) comprises a body (11), a rotation shaft (12), a first arm (13) and a second arm (14); the body (11) forms a cavity around the test vessel; the rotating shaft (12) is arranged on the body (11); the first arm (13) and the second arm (14) maintain or expand the body (11) with the rotation shaft (12) as a fulcrum.

2. The heating device according to claim 1, wherein the first arm (13) extends out of the top of the body (11); the clamp-shaped device (1) further comprises a baffle (15) arranged at the top of the body (11) and used for limiting the first arm (13).

3. The heating device according to claim 2, characterized in that the length of the first arm (13) is greater than the length of the second arm (14).

4. The device according to claim 2, characterized in that said clamp-like device (1) further comprises a support (16) and a connector (17) arranged on top of said body (11), said connector (17) being adapted to connect said baffle (15) and said support (16).

5. The heating device according to claim 1, further comprising a resilient sleeve (3) which is sleeved on the body (11).

6. A heating device according to any one of claims 1-5, characterized in that the at least one heating circuit (2) is a resistance heating circuit.

7. A heating device according to any one of claims 1-5, characterized in that the at least one heating circuit (2) is powered by an external power source.

8. A heating device according to any of claims 1-5, characterized in that the at least one heating circuit (2) extends to the bottom of the body (11) for connection to an external power source.

9. A biological sample preparation device comprising a housing, a plurality of motors, a plurality of assay containers, and a plurality of heating apparatuses according to any one of claims 1-8;

the plurality of motors are arranged in the shell, the driving shafts of the plurality of motors are respectively connected with the plurality of experimental containers to drive the plurality of experimental containers to rotate, and the plurality of heating devices are respectively clamped to heat the plurality of experimental containers.

10. The apparatus of claim 9, further comprising a controller that controls the plurality of heating devices to heat the plurality of experimental containers, respectively.