CN113566556B - Drying equipment and drying method for biological material - Google Patents
Drying equipment and drying method for biological material Download PDFInfo
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- CN113566556B CN113566556B CN202110843826.4A CN202110843826A CN113566556B CN 113566556 B CN113566556 B CN 113566556B CN 202110843826 A CN202110843826 A CN 202110843826A CN 113566556 B CN113566556 B CN 113566556B
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B25/00—Details of general application not covered by group F26B21/00 or F26B23/00
- F26B25/06—Chambers, containers, or receptacles
- F26B25/14—Chambers, containers, receptacles of simple construction
- F26B25/16—Chambers, containers, receptacles of simple construction mainly closed, e.g. drum
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Abstract
A drying device and a drying method for biological materials relate to the technical field of drying devices and comprise a bearing structure and a pressing structure which are mutually clamped and matched for fixing the biological materials; the bearing structure is provided with a plurality of first bearing parts protruding out of the bearing surface; the structure of exerting pressure has a plurality of cell bodies of keeping away from the concave establishment of the face of exerting pressure, and the cell body sets up with first bearing part one-to-one, and the degree of depth of cell body is less than the length of first bearing part protrusion loading face, under the exogenic action first bearing part with when the cell body card was held, can form airflow gap between loading face and the face of exerting pressure, and the air current of being convenient for passes through, makes biomaterial's degree of dryness more even, has guaranteed simultaneously that biomaterial's activity is not influenced, and in addition, the biomaterial atress is even, avoids taking place the shrink when dry and lead to deformation or tearing, has guaranteed the integrality of biomaterial after the drying.
Description
Technical Field
The invention relates to the technical field of drying equipment, in particular to drying equipment and a drying method for biological materials.
Background
At present, biological materials such as biological amniotic membrane, collagen membrane, etc. are mostly dried by using a freeze-drying, drying or microwave drying method. Some of the above methods use organic solvents such as DMSO, glycerol, or auxiliary solvents such as sucrose to stabilize the biological material during the lyophilization process, so as to reduce the damage of ice crystals to the biological material, and the use of these solvents may have a certain effect on the activity of the biological material. Simple oven drying or simple lyophilization can cause uneven dryness of the material, shrinkage or sticking to the drying equipment; meanwhile, the biological material is usually fixed around the plate-shaped material after being flattened, so that the biological material is easy to shrink during drying, and the middle part is torn.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the defects that the drying degree of the biological material is not uniform and the biological material is damaged during drying in the prior art, thereby providing a drying device for the biological material.
The invention is realized by the following technical scheme, and the drying equipment for the biological material comprises:
the bearing structure is provided with a plurality of first bearing parts protruding out of the bearing surface;
the pressing structure is provided with a plurality of groove bodies which are away from the pressing surface and are concavely arranged, the groove bodies are in one-to-one correspondence with the first bearing parts, the depth of each groove body is smaller than the length of each bearing surface, the first bearing parts are clamped with the groove bodies under the action of external force, and airflow gaps are formed between the bearing surfaces and the pressing surfaces.
Optionally, the bearing structure and the pressure applying structure are both provided with a plurality of air holes communicated with the air flow gap, and the air holes in the bearing structure and the pressure applying structure are arranged in a one-to-one correspondence manner.
Optionally, the air holes on the bearing structure and the pressure applying structure are uniformly distributed on the bearing surface and the pressure applying surface in rows and columns.
Optionally, the first bearing part and the groove body are provided with patterns.
Optionally, the periphery of the first bearing part is further provided with a second bearing part, and the length of the second bearing part protruding the bearing surface is smaller than the length of the first bearing part protruding the bearing surface.
Optionally, a positioning structure is disposed between the bearing structure and the pressing structure.
Optionally, the positioning structure includes a positioning pin and a positioning hole, which are matched with each other, the positioning pin is disposed at a corner of the bearing structure or the pressure applying structure, and correspondingly, the positioning hole is disposed at a corner of the pressure applying structure or the bearing structure.
Optionally, the bearing structure and the pressing structure are both plate bodies.
Optionally, the bearing structure and the pressing structure are made of stainless steel or silicone rubber.
Another technical problem to be solved by the present invention is to provide a method for drying biological material, which overcomes the disadvantages of the prior art that the drying degree of the biological material is not uniform and the biological material is damaged.
The invention can also be realized by the following technical proposal that the drying method of the biological material comprises the following steps,
the method comprises the following steps of flatly paving a pretreated biological material on a bearing surface of a bearing structure, clamping a plurality of groove bodies of a pressure applying structure, which are far away from a pressure applying surface, and a plurality of first bearing parts of the bearing structure, which protrude out of the bearing surface, in a one-to-one correspondence manner to fix the biological material, wherein an airflow gap is reserved between the pressure applying surface and the pressure bearing surface; drying the biological material at 25-37 deg.C for 8-16 hr.
The technical scheme of the invention has the following advantages:
1. according to the drying equipment for the biological material, the bearing structure is used as a carrier of the biological material, the pressure applying structure is used for clamping and fixing the biological material, because the depth of the groove body on the pressure applying structure is smaller than the length of the first bearing part on the bearing structure which protrudes out of the bearing surface, the first bearing part is clamped with the groove body under the action of external force, an airflow gap is formed between the bearing surface and the pressure applying surface, when the drying equipment is used for ventilation drying, air passes through the airflow gap, the biological material is dried, an organic solvent is not used, and the activity of the biological material is not influenced while the biological material is dried; meanwhile, as the biological material is clamped and fixed between the pressing surface and the bearing surface by the first bearing part and the groove body, the biological material is uniformly stressed, deformation or tearing caused by shrinkage during drying is avoided, and the integrity of the dried biological material is ensured.
2. According to the drying equipment for the biological materials, the bearing structure and the pressure applying structure are respectively provided with a plurality of air holes communicated with the air flow gaps, and the air holes on the bearing structure and the pressure applying structure are arranged in a one-to-one correspondence manner; the air holes on the bearing structure and the pressure applying structure are uniformly distributed on the bearing surface and the pressure applying surface in rows and columns. Through setting up the bleeder vent, the increase air current flows, has improved biomaterial's drying efficiency, makes the more even of biomaterial dryness fraction simultaneously.
3. According to the drying equipment for the biological material, the first bearing part and the groove body are respectively provided with the patterns, so that the patterns can be printed on the biological material in the drying process, the biological material is not damaged, and the printed patterns are lasting and stable.
4. According to the drying method of the biological material, the biological material is dried by ventilation by using the drying equipment of the biological material, and compared with freeze-drying in the prior art, the drying method of the biological material does not need to use an organic solvent and does not influence the biological activity; meanwhile, the pretreated biological material is flatly laid on the bearing surface of the bearing structure, and the biological material is fixed by mutually clamping the plurality of groove bodies of the pressure applying structure and the plurality of first bearing parts of the bearing structure in a one-to-one correspondence manner, so that the biological material is uniformly stressed, and the shape change caused by shrinkage due to nonuniform deformation of the biological material during drying is prevented; and an airflow gap is reserved between the pressure application face and the pressure bearing face, compared with the existing method for directly drying the biological material exposed outside, the method for drying the biological material by ventilating and drying the biological material by utilizing the airflow gap has the advantages that the dryness of the biological material is more uniform, the biological material can be protected from being damaged, and the integrity of the biological material is ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a load-bearing structure according to a first embodiment;
FIG. 2 is a schematic view of a press structure cooperating with the load bearing structure shown in FIG. 1;
FIG. 3 is a front view of a load bearing structure in a second embodiment;
an enlarged view of the structure at a in fig. 3 in fig. 4;
FIG. 5 is a top view of the load bearing structure shown in FIG. 3;
FIG. 6 is an enlarged view of the structure at B in FIG. 5;
FIG. 7 is a left side view of the load bearing structure shown in FIG. 3;
FIG. 8 is a top view of a press structure mated with the load bearing structure shown in FIG. 3;
FIG. 9 is a front view of the pressing structure shown in FIG. 8;
fig. 10 is an enlarged view of the structure at C in fig. 9.
Description of reference numerals:
1-a load bearing structure; 11-a first carrier part; 12-a second carrier part; 2-a pressure applying structure; 21-a groove body; 3, air holes; 4-positioning structure.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
Fig. 1 shows an embodiment of the drying apparatus for biological material, which is combined with fig. 2, and comprises a support structure 1 and a pressing structure 2 for fixing biological material by snap-fitting with each other. The bearing structure 1 is provided with a plurality of first bearing parts 11 protruding out of a bearing surface; structure 2 of exerting pressure has a plurality of cell bodies 21 of keeping away from the concave establishment of pressure face, cell body 21 with first bearing part 11 one-to-one sets up, just the degree of depth of cell body 21 is less than first bearing part 11 protrusion the length of loading end, like this, through exogenic action with first bearing part 11 with when cell body 21 card is held the loading end with can form airflow gap between the pressure face, make things convenient for passing through of biomaterial air current in the ventilation drying process for biomaterial's degree of dryness is more even. In order to increase ventilation and improve the drying efficiency and drying uniformity of the biological material, a plurality of air holes 3 communicated with air flow gaps are formed in the bearing structure 1 and the pressure applying structure 2, and the air holes 3 in the bearing structure 1 and the pressure applying structure 2 are arranged in a one-to-one correspondence manner. When the first bearing part of the bearing structure 1 and the groove body 21 of the pressure applying structure 2 are clamped in a one-to-one correspondence manner, the vent holes 3 on the bearing structure 1 and the pressure applying structure 2 are also in correspondence, so that air circulation is facilitated. Meanwhile, the air holes 3 on the bearing structure 1 and the pressure applying structure 2 are uniformly distributed on the bearing surface and the pressure applying surface in rows and columns, so that the drying uniformity of the biological material is further improved.
Fig. 3 shows another embodiment of the drying apparatus for biological material, and with reference to fig. 4-10, patterns are provided on both the first bearing part 11 and the trough body 21. The present embodiment is UP letters.
The periphery of the first bearing part 11 is further provided with a second bearing part 12, and the length of the second bearing part 12 protruding the bearing surface is smaller than the length of the first bearing part 11 protruding the bearing surface. In this way, the second bearing part 12 can play a role of assisting in fixing the biomaterial, and at the same time, because the length of the second bearing part 12 protruding the bearing surface is shorter than the length of the first bearing part 11 protruding the bearing surface, no pattern can be printed on the biomaterial.
In order to fix the relative position between the bearing structure 1 and the pressing structure 2, a positioning structure 4 is further arranged between the bearing structure 1 and the pressing structure 2.
Specifically, the positioning structure 4 includes a positioning pin and a positioning hole, which are matched with each other, the positioning pin is disposed at a corner of the bearing structure 1, and correspondingly, the positioning hole is disposed at a corner of the pressing structure 2. Of course, the positioning pin may also be disposed at a corner of the pressing structure 2, and correspondingly, the positioning hole is disposed at a corner of the bearing structure 1.
The bearing structure 1 and the pressing structure 2 are both plate bodies.
Specifically, the bearing structure 1 and the pressing structure 2 are made of metal, rubber or plastic, such as stainless steel, silicone rubber, nylon, polytetrafluoroethylene, wherein the stainless steel or the silicone rubber is preferred, and the silicone rubber hardness is preferably 60-80 shore a. The bearing structure 1 and the pressing structure 2 in the embodiment are made of stainless steel, so that the processing precision is high, and the printed patterns are finer. Certainly, silicon rubber can be selected, the processing precision of the silicon rubber is high without stainless steel equipment, but the length of the first bearing part 11 protruding out of the bearing surface is longer, the depth of the groove body 21 far away from the pressing surface is deeper, the inertia of the silicon rubber material is better, and the silicon rubber has the characteristic that the biological material is not adhered to drying equipment after being dried.
Example 2
In the prior art, the drying method of the biological material generally comprises the steps of directly placing the biological material such as biological amniotic membrane, collagen membrane and the like on a tray in an electric heating air blast drying box for drying, wherein the biological material is shrunk due to drying to form an uneven membrane. The use of a simple clamp can result in uneven drying of the biological material. Or the biological material is leveled, and the periphery of the biological material is fixed on the plate-shaped equipment. Because the fixing force points are around, the biomaterial shrinks during drying and is easy to tear at the middle part. In addition, although it is necessary to press patterns such as patterns on the dried biomaterial, and then perform processes such as cutting, packaging, and sterilization, the simple method using the copper stamp and the steel stamp causes unnecessary contamination or damage to the biomaterial, and the pressed patterns such as patterns gradually become flat or disappear with time due to the toughness of the biomaterial.
In view of the above problems, the present invention provides a method for drying a biomaterial, comprising the steps of,
laying the pretreated biological material on a bearing surface of a bearing structure 1, correspondingly and mutually clamping a plurality of groove bodies 21 of a pressure applying structure 2, which are far away from the pressure applying surface, and a plurality of first bearing parts 11 of the bearing structure 1, which protrude out of the bearing surface, one by one so as to fix the biological material, and reserving an air flow gap between the pressure applying surface and the bearing surface; drying the biological material at 25-37 deg.C for 8-16 hr.
Compared with the traditional drying method, the drying method can uniformly dry the biological material, the biological material is uniformly stressed in the drying process, and the biological material cannot become irregular due to the shrinkage of the biological material and even be torn due to deformation. In the drying process, the drying equipment and the biological material are not seriously adhered due to small stressed contact surface so that the biological material cannot be removed after the drying is finished, the bearing structure 1 and the pressing structure 2 take patterns such as patterns as an acting point when being clamped, the effects of the patterns and the patterns are generated while the drying is carried out, and the patterns on the surface of the material cannot disappear along with the prolonging of the storage time of the material.
One embodiment of a method for drying biological material, comprising the steps of,
the method comprises the steps of flatly paving a biological amniotic membrane material processed by washing and other steps on a bearing surface of a bearing structure 1, correspondingly and mutually clamping a groove body 21 of a pressure applying structure 2 and a first bearing part 11 of the bearing structure 1 one by one, loading positioning saltpeter or clamping other positioning devices for fixing, and clamping the biological amniotic membrane material between the bearing structure 1 and the pressure applying structure 2. Since the length of the first bearing part 11 protruding the bearing surface is greater than the depth of the groove body 21, an air flow gap through which air passes is left between the bearing surface and the pressing surface. The drying equipment with the biological amnion placed is placed in an electric heating blast drying box (Boxun GZX-9023MBE) and dried for 12 hours at the temperature of 37 ℃ or dried for 16 hours at the temperature of 25 ℃, and then the biological amnion is taken out, thus obtaining the flat biological amnion material with the pattern printed, the water content of which is less than 5 percent. The water content was measured using the loss on drying method.
Another embodiment of the method for drying biological material comprises the steps of,
the collagen membrane material processed by the steps of washing, crosslinking and the like is flatly laid on the bearing surface of the bearing structure 1, the groove body 21 of the pressure applying structure 2 and the first bearing part 11 of the bearing structure 1 are correspondingly clamped with each other one by one, positioning saltpeter is arranged in the groove body, or other positioning devices are clamped, and the collagen membrane material is clamped between the bearing structure 1 and the pressure applying structure 2. Since the length of the first bearing part 11 protruding the bearing surface is greater than the depth of the groove body 21, an air flow gap through which air passes is left between the bearing surface and the pressing surface. The drying equipment with the collagen film is placed in an electric heating blast drying oven (Boxun GZX-9023MBE), dried for 8 hours at the temperature of 37 ℃, and then the collagen film is taken out, thus obtaining the smooth collagen film material with the water content less than 5 percent and printed with the pattern.
In yet another embodiment of the method for drying biological material, the method comprises the steps of,
the gelatin film material processed by the steps of washing, crosslinking and the like is flatly paved on the bearing surface of the bearing structure 1, the groove body 21 of the pressure applying structure 2 and the first bearing part 11 of the bearing structure 1 are correspondingly clamped with each other one by one, positioning saltpeter is loaded in the groove body, or other positioning devices are clamped, and the gelatin film material is clamped between the bearing structure 1 and the pressure applying structure 2. Since the length of the first bearing part 11 protruding the bearing surface is greater than the depth of the groove body 21, an air flow gap through which air passes is left between the bearing surface and the pressing surface. The drying equipment with the gelatin film placed is placed in an electric heating blowing drying oven (Boxun GZX-9023MBE), the temperature is set to be 25 ℃, the drying is carried out for 12 hours, and then the gelatin film is taken out, so that the relatively flat gelatin film material with the water content of less than 5 percent and the printed pattern can be obtained.
In yet another embodiment of the method for drying biological material, the method comprises the steps of,
the dried biomaterial of any of the above examples was radiation sterilized, packaged with blisters and plastic-aluminum bags, and sterilized at a minimum of 17.5 kGy. After sterilization, the quality guarantee period can reach more than 2 years through sterility, drying weight loss, endotoxin content, physicochemical property, biological property and other detection, and the printed patterns and other patterns can exist for a long time.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (11)
1. An apparatus for drying biological material, comprising:
the bearing structure is provided with a plurality of first bearing parts protruding out of the bearing surface;
the pressing structure is provided with a plurality of groove bodies which are away from the pressing surface and are concavely arranged, the groove bodies are in one-to-one correspondence with the first bearing parts, the depth of each groove body is smaller than the length of each bearing surface, the first bearing parts are clamped with the groove bodies under the action of external force, and airflow gaps are formed between the bearing surfaces and the pressing surfaces.
2. The drying apparatus for biological material according to claim 1, wherein the bearing structure and the pressing structure are respectively provided with a plurality of air holes communicated with the air flow gap, and the air holes of the bearing structure and the pressing structure are correspondingly arranged.
3. The apparatus for drying biological material according to claim 2, wherein the air holes of the support structure and the pressing structure are uniformly distributed in rows and columns on the support surface and the pressing surface.
4. The drying apparatus for biomaterial as claimed in any one of claims 1 to 3, wherein the first bearing portion and the trough body are provided with patterns.
5. The drying apparatus for biomaterial according to any one of claims 1 to 3, wherein the first bearing part is further provided with a second bearing part at the periphery, and the length of the convex bearing surface of the second bearing part is less than that of the convex bearing surface of the first bearing part.
6. The drying apparatus for biological material according to claim 4, wherein the first bearing part is further provided with a second bearing part at the periphery thereof, and the length of the convex bearing surface of the second bearing part is smaller than that of the convex bearing surface of the first bearing part.
7. Drying apparatus for biological material according to any of claims 1-3, characterised in that a positioning structure is provided between the carrying structure and the pressure application structure.
8. The drying apparatus for biomaterial as claimed in claim 7, wherein the positioning structure comprises a positioning pin and a positioning hole which are matched with each other, the positioning pin is arranged at the corner of the bearing structure or the pressing structure, and correspondingly, the positioning hole is arranged at the corner of the pressing structure or the bearing structure.
9. Drying apparatus for biological material according to any of claims 1-3, characterised in that the carrying structure and the pressing structure are both plate bodies.
10. Drying apparatus for biological material according to any of claims 1-3, characterised in that the carrying structure and the pressure exerting structure are made of stainless steel or silicone rubber.
11. A drying method of a biomaterial using the drying apparatus of the biomaterial according to any one of claims 1 to 10, comprising the steps of,
the method comprises the following steps of flatly paving a pretreated biological material on a bearing surface of a bearing structure, clamping a plurality of groove bodies of a pressure applying structure, which are far away from a pressure applying surface, and a plurality of first bearing parts of the bearing structure, which protrude out of the bearing surface, in a one-to-one correspondence manner to fix the biological material, wherein an airflow gap is reserved between the pressure applying surface and the bearing surface; drying the biological material at 25-37 deg.C for 8-16 hr.
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| EP2578976A1 (en) * | 2011-10-06 | 2013-04-10 | Sanofi Pasteur Sa | Rotary drum for use in a vacuum freeze-dryer |
| LU92648B1 (en) * | 2015-02-04 | 2016-08-05 | Project Pharmaceutics Gmbh | Method and device for optimized freeze-drying of a pharmaceutical product |
| CN104888269B (en) * | 2015-05-05 | 2017-04-12 | 上海大学 | Method for increasing interface bonding force of HA/ZrO2 layered composite biological ceramic material |
| CN107702526A (en) * | 2017-09-22 | 2018-02-16 | 北京大清生物技术股份有限公司 | A kind of biological sticking patch drying device |
| CN210832769U (en) * | 2019-09-30 | 2020-06-23 | 陕西佰傲再生医学有限公司 | Freeze-drying mold for acellular matrix material |
| CN211503446U (en) * | 2019-12-16 | 2020-09-15 | 杭州恩格生物医疗科技有限公司 | Biological amnion freeze-drying device and system thereof |
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