CN113559310A

CN113559310A - Preparation method and equipment of hemostatic material for war wounds

Info

Publication number: CN113559310A
Application number: CN202110813272.3A
Authority: CN
Inventors: 李大伟; 张旭; 陶笙; 李春宝; 周瑾; 王星; 廖玉辉; 罗展鹏; 鲍达; 徐雄
Original assignee: 8th Medical Center of PLA General Hospital
Current assignee: 8th Medical Center of PLA General Hospital
Priority date: 2021-07-19
Filing date: 2021-07-19
Publication date: 2021-10-29
Anticipated expiration: 2041-07-19
Also published as: CN113559310B

Abstract

The invention discloses a preparation method of a hemostatic material for war wounds, which comprises an electrostatic spinning outer membrane and natural polysaccharide hydrogel, wherein the natural polysaccharide hydrogel is conveniently covered on tissues around the wounds of patients for isolation through the electrostatic spinning membrane, so that the tissues around the wounds are prevented from being adhered, pollutants can be prevented from entering the hydrogel, and meanwhile, the electrostatic spinning membrane is convenient for removing the natural polysaccharide hydrogel; through the hemostatic material consisting of the electrostatic spinning membrane and the natural polysaccharide hydrogel, the natural polysaccharide hydrogel has a good hemostatic effect, on one hand, the hydrogel material can generate instantaneous sol-gel transformation, and can realize physical plugging of bleeding points so as to stop bleeding; on the other hand, the hydrogel material has a biological function hemostasis effect, because the chitosan has a certain amount of charges, the molecules of the chitosan can directly activate the blood platelets on the wound surface to initiate the blood coagulation reaction, and the blood coagulation is promoted, so that the hemostasis effect is achieved.

Description

Preparation method and equipment of hemostatic material for war wounds

Technical Field

The invention relates to the technical field of biomedical materials, in particular to a preparation method of a hemostatic material for war wounds and equipment thereof.

Background

The traditional tourniquet and the pressure bandaging hemostasis method have the defects of uncertain hemostasis effect, ischemic necrosis of limbs and the like, and are not suitable for being used as a first choice method for emergency treatment hemostasis. How to rationally construct novel first aid hemostasis material to different bleeding wounds, realize the quick shutoff of bleeding wound, strive for more time for subsequent treatment, become the problem that the letter awaits the solution. In recent years, many researches on novel emergency hemostatic materials are carried out, and the hydrogel material has the functional characteristics of semi-permeability, wound infection prevention, oxygen and water passing permission and the like due to the specific polymer network structure, so that the hydrogel material becomes one of the important development directions of the emergency hemostatic materials. Meanwhile, the self-rescue mutual-rescue material is provided as a high-efficiency and convenient self-rescue mutual-rescue material for war wounds wounded personnel, and has very important significance for reducing the death rate and improving the war wound rescue level. Therefore, there is a need for a method and apparatus for preparing hemostatic material for war wound to at least partially solve the problems of the prior art.

Disclosure of Invention

In this summary, concepts in a simplified form are introduced that are further described in the detailed description. This summary of the invention is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.

In order to at least partially solve the above problems, the present invention provides a method for preparing a hemostatic material for war wounds, the hemostatic material comprising an electrospun outer membrane and a natural polysaccharide hydrogel for coating on a surface of the electrospun outer membrane to rapidly gel with the electrospun outer membrane, the method comprising:

1) preparing a preparation material, namely selecting polyethylene glycol amino or polyethylene glycol-succinimide ester as the preparation material of the electrostatic spinning outer membrane; selecting a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle as preparation materials of the hydrogel;

2) preparing an electrostatic spinning outer membrane, namely preparing the polyethylene glycol amino or polyethylene glycol-succinimide ester in the step 1 into electrostatic spinning liquid; then, by an electrostatic spinning technology, electrostatic spinning is carried out under a receiving electric field, and the electrostatic spinning is gathered into a plurality of groups of first fiber bundles, the first fiber bundles and the first fiber bundles are mutually staggered to form a plurality of groups of grid second fiber bundles and membrane body fibers positioned between the grids, so that an electrostatic spinning outer membrane is prepared;

3) preparing natural polysaccharide hydrogel, firstly obtaining a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle respectively; then respectively preparing a solution A containing chitosan derivatives and a solution B containing aldehyde-based natural polysaccharides, modified block copolymer micelles and photoinitiators; and finally, mixing the solution A and the solution B, adding a cross-linking agent and thrombin, and mixing and reacting at room temperature to obtain the natural hydrogel.

The invention provides a preparation device of a hemostatic material for war wounds, which comprises: the electrostatic spinning device is used for preparing an electrostatic spinning outer diaphragm and is positioned on the back face of the fastening mechanism, a fixing plate is arranged on the inner bottom wall of the electrostatic spinning device, a first connecting rod is arranged on the inner front wall of the electrostatic spinning device, a liquid storage tank is arranged at one end of the first connecting rod, a transverse seat plate is arranged on the inner bottom wall of the electrostatic spinning device and is positioned on one side of the fixing plate, a transmitter is arranged at the top of the transverse seat plate, a liquid conveying pipe penetrates through the bottom of the liquid storage tank, one end of the liquid conveying pipe penetrates through the outer wall of the transmitter, an electric valve is arranged on the outer wall of the liquid conveying pipe, a clamping groove is formed in the inner wall of the transmitter, a concave plate is arranged at the top of the transmitter, a second pressure sensor is arranged at the top of the concave plate, a round hole is formed in the bottom of the concave plate, and a small cylinder is arranged at the top of the transmitter in a penetrating manner, and one end of the small cylinder extends into the clamping groove, the outer wall of the small cylinder is provided with a limiting block, and the bottom of the small cylinder is provided with a floating plate.

According to the preparation equipment of the hemostatic material for war wounds, the top of the first support plate is provided with the driving box and the antibacterial cover, the antibacterial cover is positioned on the front side of the electrostatic spinning device, the inner wall of the antibacterial cover is provided with the first disinfection lamp, the driving box is positioned in the antibacterial cover, the top of the driving box is provided with the support cylinder, the inner bottom wall of the driving box is provided with the driving motor, the output end of the driving motor is provided with the lead screw, one end of the lead screw extends into the support cylinder, the outer wall of the support cylinder is provided with the chute, the outer wall of the lead screw is provided with the sliding plate, one end of the sliding plate penetrates through the inside of the chute, the outer wall of the sliding plate is provided with the moving sleeve, the outer wall of the moving sleeve is provided with the second connecting rod, one end of the second connecting rod is provided with the support disc, and the top of the support disc is provided with the rotating motor, the output of rotating electrical machines is provided with the stirring rod, support cylindrical outer wall and be provided with first pressure sensor, the below of stirring rod is provided with a container section of thick bamboo.

According to the preparation device of the hemostatic material for war wounds, disclosed by the embodiment of the invention, the transparent box door is installed on the outer wall of the electrostatic spinning device, the second disinfection lamp is installed on the inner top wall of the electrostatic spinning device, the high-voltage power supply is installed on the inner bottom wall of the base, the hydraulic push rod is installed on the inner wall of the electrostatic spinning device, the movable piston is installed at one end of the hydraulic push rod, the liquid spraying port is installed at the top of the emitter in a penetrating mode, and the liquid spraying port is electrically connected with the high-voltage power supply through a lead.

According to the preparation device of the hemostatic material for war wounds, the receiving screen is arranged on the inner bottom wall of the fixing plate and is electrically connected with the high-voltage power supply through the conducting wire, the moving rod penetrates through the outer wall of the fixing plate, and the first spring is arranged on the outer wall of the moving rod in a surrounding mode.

According to the preparation device of the hemostatic material for war wounds, provided by the embodiment of the invention, the top of the first support plate is provided with the fixed cylinder, the top of the first support plate is provided with the connecting column, the connecting column is positioned on the back surface of the fixed cylinder, the outer wall of the connecting column is provided with the sliding sleeve, the outer wall of the connecting column is provided with the mounting block, the mounting block is positioned below the sliding sleeve, the outer wall of the sliding sleeve is provided with the third connecting rod, one end of the third connecting rod is provided with the connecting disc, the inner wall of the fixed cylinder is provided with the beaker, the top of the connecting disc is provided with the catheter in a penetrating mode, and one end of the catheter extends into the beaker.

According to the preparation device of the hemostatic material for war wounds, the fastening mechanism comprises the transverse electric rod, the rubber block and the vertical rod, the fastening mechanism is arranged on two sides of the container barrel respectively, the vertical rod is arranged on the first supporting plate, the transverse electric rod is horizontally and rotatably arranged on the vertical rod, and the rubber block is arranged on one end, facing the container barrel, of the transverse electric rod and abuts against the container barrel.

According to the preparation device of the hemostatic material for war wounds, the top of the first support plate is provided with the placing cylinder, and the measuring cylinder is arranged in the placing cylinder.

According to the preparation device of the hemostatic material for war wounds, the placing cylinder is internally provided with a bearing mechanism for bearing the measuring cylinder, the bearing mechanism comprises a second supporting plate, a supporting cylinder, a second spring and an intermediate bearing plate, the supporting cylinder is arranged on the second supporting plate, a spring groove is formed in the second supporting plate, the second spring is arranged in the spring groove, the intermediate bearing plate is arranged on the second spring, a plurality of first hinge seats are arranged around the second spring, the first hinge seats are hinged with the intermediate bearing plate through first hinge rods, second hinge seats corresponding to the first hinge seats are arranged on the inner wall of the supporting cylinder, vertical guide grooves are formed in the inner wall of the supporting cylinder, the second hinge seats are positioned in the vertical guide grooves, and the second hinge seats are hinged with the first seats through second hinge rods, the upper end of the supporting cylinder is provided with a top plate, the middle bearing plate is provided with an upper fixing column, the upper end of the upper fixing column extends out of the top plate, the measuring cylinder is arranged on the upper fixing column, the upper fixing column is provided with a fixing groove corresponding to the measuring cylinder, the second hinge seat is connected with a third hinge seat through a third hinge rod, the third hinge seat is positioned at the bottom of the top plate, the third hinge seat is provided with a propping seat for propping against the upper fixing column, the cylinder wall of the supporting cylinder is internally provided with an inner vertical groove, the inner vertical groove is positioned at the inner side of the vertical guide groove, the bottom of the inner vertical groove is provided with a third spring, the third spring is provided with a vertical supporting rod, the second hinge seat is provided with a plurality of wedge-shaped blocks, and the vertical supporting rod is provided with a wedge-shaped rod corresponding to the wedge-shaped blocks, and the upper end of the vertical supporting rod is provided with a top column, and the top column is connected with the top plate.

Compared with the prior art, the invention at least comprises the following beneficial effects:

the invention provides a preparation method of a hemostatic material for war wounds, which comprises an electrostatic spinning outer membrane and natural polysaccharide hydrogel, wherein the natural polysaccharide hydrogel is conveniently covered on tissues around the wounds of patients for isolation through the electrostatic spinning membrane, so that the tissues around the wounds are prevented from being adhered, pollutants can be prevented from entering the hydrogel, and meanwhile, the electrostatic spinning membrane is convenient for removing the natural polysaccharide hydrogel; the hemostatic material composed of the electrostatic spinning membrane and the natural polysaccharide hydrogel can keep ideal strength, protect wounds, control bleeding, prevent infection and be used as a secretion absorbing dressing; on one hand, the hydrogel material can generate instant sol-gel transformation, and can realize physical plugging of bleeding points so as to stop bleeding; on the other hand, the hydrogel material has a biological function hemostasis effect, because the chitosan has a certain amount of charges, the molecules of the chitosan can directly activate the blood platelets on the wound surface to initiate the blood coagulation reaction, and the blood coagulation is promoted, so that the hemostasis effect is achieved.

Other advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic flow diagram of the process of the present invention;

FIG. 2 is a schematic structural view of a hemostatic material of the present invention;

FIG. 3 is a schematic structural view of an electrospun outer membrane of the present invention;

FIG. 4 is a schematic structural view of the apparatus of the present invention;

FIG. 5 is a schematic structural view of a fixed cylinder according to the present invention;

FIG. 6 is a schematic view of the structure of the driving motor according to the present invention;

FIG. 7 is a schematic view of the fastening mechanism of the present invention;

FIG. 8 is a schematic view showing an internal structure of an electrospinning device according to the present invention;

FIG. 9 is a schematic diagram of a second pressure sensor according to the present invention;

FIG. 10 is a schematic structural view of a carrying mechanism according to the present invention;

FIG. 11 is a partial structural view of the loading mechanism of the present invention;

FIG. 12 is a partial structural view of a support cylinder according to the present invention;

FIG. 13 is a top view of the structure of the load bearing mechanism of the present invention;

fig. 14 is a schematic structural view of the robot arm mechanism of the present invention.

Detailed Description

The present invention is further described in detail below with reference to the drawings and examples so that those skilled in the art can practice the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

As shown in fig. 1 to 3, the present invention provides a method for preparing a hemostatic material for war wounds, the hemostatic material comprising an electrospun outer membrane 100 and a natural polysaccharide hydrogel 200 for coating the surface of the electrospun outer membrane 100 and rapidly gelling the electrospun outer membrane 100, the method comprising:

1) preparing materials, namely selecting polyethylene glycol amino or polyethylene glycol-succinimidyl ester as the materials for preparing the electrostatic spinning outer membrane 100; selecting a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle as preparation materials of the hydrogel 200;

2) preparing an electrostatic spinning outer membrane 100, and preparing the polyethylene glycol amino or polyethylene glycol-succinimidyl ester in the step 1 into electrostatic spinning solution; then, by an electrostatic spinning technology, electrostatic spinning is carried out under a receiving electric field, and the electrostatic spinning is gathered into a plurality of groups of first fiber bundles 300, the first fiber bundles 300 are mutually staggered to form a plurality of groups of second fiber bundles 400 of grids and membrane fibers 500 positioned among the grids, so that the electrostatic spinning outer membrane 100 is prepared;

3) preparing a natural polysaccharide hydrogel 200, firstly obtaining a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle respectively; then respectively preparing a solution A containing chitosan derivatives and a solution B containing aldehyde-based natural polysaccharides, modified block copolymer micelles and photoinitiators; and finally, mixing the solution A and the solution B, adding a cross-linking agent and thrombin, and mixing and reacting at room temperature to obtain the natural hydrogel 200.

As shown in fig. 4 to 9, the present invention also provides a device for preparing a hemostatic material for war wound, which is used for preparing the electrospun outerwrap 100, and mixing the solution a and the solution B with a cross-linking agent and thrombin, followed by stirring to prepare a natural hydrogel 200.

The preparation equipment comprises a first supporting plate 1, a fixed cylinder 2 and a driving box 4, wherein the fixed cylinder 2 is installed at the top of the first supporting plate 1, the first supporting plate 1 supports the fixed cylinder 2 at the top to realize a supporting function, the fixed cylinder 2 plays a role in fixing a beaker to prevent the beaker from deviating, the driving box 4 and an antibacterial cover 41 are arranged at the top of the first supporting plate 1, the antibacterial cover 41 is positioned on the front surface of an electrostatic spinning device 5, the electrostatic spinning device 5 is used for preparing an electrostatic spinning outer membrane 100, a first disinfection lamp 42 is installed on the inner wall of the antibacterial cover 41, the driving box 4 is positioned in the antibacterial cover 41, the antibacterial cover 41 provides an installation position for the first disinfection lamp 42, the first disinfection lamp 42 sterilizes components used for preparing hydrogel in the antibacterial cover 41, the hydrogel preparation process is in an aseptic environment, and the first disinfection lamp 42 still works for a period of time after the hydrogel preparation is finished, will be shut down;

the top of the driving box 4 is provided with a supporting cylinder 401, the bottom wall inside the driving box 4 is provided with a driving motor 402, and the driving box 4 provides a mounting position for the driving motor 402; the output end of the driving motor 402 is provided with a screw rod 403, one end of the screw rod 403 extends into the supporting cylinder 401, the outer wall of the supporting cylinder 401 is provided with a sliding groove, the outer wall of the screw rod 403 is provided with a sliding plate, one end of the sliding plate penetrates through the sliding groove, the outer wall of the sliding plate is provided with a moving sleeve 404, the outer wall of the moving sleeve 404 is provided with a second connecting rod, one end of the second connecting rod is provided with a supporting disc, the top of the supporting disc is provided with a rotating motor 405, the output end of the rotating motor 405 is provided with a stirring rod 406, the outer wall of the supporting cylinder 401 is provided with a first pressure sensor 407, and a container 408 is arranged below the stirring rod 406;

mixing the solution A and the solution B to prepare a mixed solution, adding a cross-linking agent and thrombin into the mixed solution in a container barrel 408, stirring, driving a screw rod 403 to rotate by a driving motor 402, converting the self rotary motion of the screw rod 403 into the linear motion of a sliding plate, driving a movable sleeve 404 to move downwards by the sliding plate, driving a connecting rod and a supporting disc to move downwards by the movable sleeve 404, when the movable sleeve 404 touches the top of a first pressure sensor 407, starting a rotary motor 405 by the first pressure sensor 407, driving a stirring rod 406 to rotate by the rotary motor 405, stirring the mixed solution in the container barrel 408 by the stirring rod 406, accelerating the reaction rate, shortening the preparation time of hydrogel and realizing the function of shortening the preparation time, when stirring is not needed, driving the motor 402 to rotate reversely, driving the movable sleeve 404 to move upwards, when the movable sleeve 404 is not pressing the first pressure sensor 407, rotating electrical machines 405 stall, first pressure sensor 407 plays the function of control rotating electrical machines 405 operating condition, and the spout plays spacing effect to the slide for the slide can only be along spout up-and-down motion, and the connecting rod provides the hookup location for supporting the disc, supports the rotating electrical machines 405 at disc to the top and supports the function.

Further, an electrostatic spinning device 5 is installed on the top of the first support plate 1, a fixing plate 6 is installed on the inner bottom wall of the electrostatic spinning device 5, a connecting rod is installed on the inner front wall of the electrostatic spinning device 5, a liquid storage tank 7 is installed at one end of the connecting rod, a transverse seat plate is installed on the inner bottom wall of the electrostatic spinning device 5 and is located on one side of the fixing plate 6, an emitter 503 is installed on the top of the transverse seat plate, a liquid conveying pipe is installed at the bottom of the liquid storage tank 7 in a penetrating mode, one end of the liquid conveying pipe penetrates through the outer wall of the emitter 503, an electric valve 701 is installed on the outer wall of the liquid conveying pipe, a clamping groove is formed in the inner wall of the emitter 503, a concave plate 702 is installed on the top of the emitter 503, a second pressure sensor 703 is installed on the top of the concave plate 702, a round hole is formed in the bottom of the concave plate 702, a small cylinder is installed on the top of the emitter 503 in a penetrating mode, and one end of the small cylinder extends into the clamping groove, the outer wall of the small cylinder is provided with a limiting block 705, the bottom of the small cylinder is provided with a floating plate 704, when the electrostatic spinning solution is supplemented into the emitter 503, a hydraulic push rod 504 drives a piston to move backwards, the floating plate 704 positioned in the clamping groove drives the small cylinder to move downwards, the limiting block 705 plays a limiting role on the small cylinder to prevent the small cylinder from continuously descending, the top of the small cylinder does not extrude the second pressure sensor 703, the second pressure sensor 703 starts an electric valve 701, the electric valve 701 is opened, the electrostatic spinning solution in the liquid storage tank 7 flows into the emitter 503 through a liquid conveying pipe, the floating plate 704 rises along with the rise of the liquid level until the floating plate 704 enters the clamping groove again, the small cylinder moves upwards and extrudes the second pressure sensor 703, the second pressure sensor 703 senses the pressure change and closes the electric valve 701, the electrostatic spinning solution can be automatically supplemented into the emitter through the electric valve 701 and the second pressure sensor 703, realize the function of automatic replenishment electrostatic spinning liquid, electrostatic spinning liquid is formed with the preparation of polyethylene glycol amino, and electrostatic spinning ware 5 provides the mounted position for fixed plate 6, and the connecting rod provides hookup location for liquid reserve tank 7, and the horizontal bedplate supports the transmitter 503 at top, realizes the support function, and the small circle post passes concave plate 702 through the round hole and extrudees second pressure sensor 703.

Further, a placing cylinder 104 is installed on the top of the first supporting plate 1, the placing cylinder 104 provides a placing space for the measuring cylinder 105, and the measuring cylinder 105 can measure the volume of the required solution.

Further, a connecting column 201 is installed at the top of the first support plate 1, the connecting column 201 is located on the back of the fixed cylinder 2, a sliding sleeve 202 is installed on the outer wall of the connecting column 201, an installation block is installed on the outer wall of the connecting column 201 and located below the sliding sleeve 202, a connecting rod is installed on the outer wall of the sliding sleeve 202, a connecting disc is installed at one end of the connecting rod, a beaker 206 is installed on the inner wall of the fixed cylinder 2, a guide tube 203 is installed on the top of the connecting disc in a penetrating manner, and one end of the guide tube 203 extends into the beaker 206, when polyethylene glycol amino or polyethylene glycol-succinimidyl ester is prepared into a solution, polyethylene glycol amino or polyethylene glycol-succinimidyl ester with a volume measured by the measuring cylinder 105 is added into the beaker 206 through the guide tube 203, then a proper amount of clear water is measured and poured into the beaker through the guide tube 203, and the sliding sleeve 202 is lifted upwards, the sliding sleeve 202 drives the connecting rod and the connecting disc to move upwards, the bottom of the guide pipe 203 leaves the interior of the beaker 206, the beaker 206 is taken out, polyethylene glycol amino or polyethylene glycol-succinimide ester solution is filled in the beaker 206, the function of preparing the polyethylene glycol amino or polyethylene glycol-succinimide ester solution is realized, and the mounting block plays a limiting role in the sliding sleeve 202 and limits the moving range of the sliding sleeve 202.

Further, the fastening mechanism comprises a transverse electric rod 101, a rubber block 102 and a vertical rod 103, the fastening mechanism is arranged on each of two sides of the container barrel 408, the vertical rod 103 is arranged on the first supporting plate, the transverse electric rod 101 is horizontally and rotatably arranged on the vertical rod 103, the rubber block 102 is arranged at one end, facing the container barrel 408, of the transverse electric rod 101 and abuts against the container barrel 408, specifically, the transverse electric rod 101 is installed on the front side of the vertical rod 103 in a penetrating manner, the rubber block 102 is installed at one end of the transverse electric rod 101, the transverse electric rod 101 provides an installation position for the rubber block 102, and the rubber block 102 fixes the container barrel 408 filled with the polyethylene glycol amino solution, so that the container barrel 408 is prevented from severely shaking to cause splashing of the solution in the stirring process; when the driving motor 402 drives the screw rod 403 to rotate, so that the stirring rod 406 on the rotating motor 405 moves downwards, the transverse electric rod 101 pushes the rubber block 102 to approach the container barrel 408 to tightly fix the container barrel 408, and then the stirring rod 406 enters the container barrel 408 for stirring; after the stirring is completed, the rotating motor 405 stops rotating, further the stirring rod 406 stops rotating, the driving motor 402 drives the screw rod 403 to rotate, so that the rotating motor 405 and the stirring rod 406 move upwards, and meanwhile, the transverse electric rod 101 also drives the rubber block 102 to be far away from the container barrel 408, so that when the solution is mixed, the stirring action of the stirring rod 406 and the clamping or loosening action of the fastening mechanism are combined, and the use efficiency is improved.

Further, a transparent box door 501 is installed on the outer wall of the electrostatic spinning device 5, a second disinfection lamp 506 is installed on the inner top wall of the electrostatic spinning device 5, a high-voltage power supply 502 is installed on the inner bottom wall of the transverse seat plate, a hydraulic push rod 504 is installed on the inner wall of the electrostatic spinning device 5, a moving piston is installed at one end of the hydraulic push rod 504, a liquid spraying port 505 is installed on the top of the emitter 503 in a penetrating mode, the liquid spraying port 505 is electrically connected with the high-voltage power supply 502 through a lead, when the electrostatic spinning outer membrane is manufactured, the high-voltage power supply 502 enables high-voltage electricity to be conducted to the liquid spraying port 505 and the receiving screen 601, the hydraulic push rod 504 drives the piston to move forwards, the piston pushes the electrostatic spinning liquid inside the emitter forwards, liquid drops at the liquid spraying port 505 are changed into a conical shape from a spherical shape, fiber filaments are obtained by extending from the conical tip end of the conical shape, the fiber filaments fall on the receiving screen 601, and the operations are repeated, and finally a plurality of groups of first fiber bundles, a plurality of the first fiber bundles, a second disinfection lamp can be obtained, The electrostatic spinning outer membrane formed by the die body fibers and the second fiber bundles achieves the function of manufacturing the electrostatic spinning outer membrane, the electrostatic spinning device 5 can be opened by the transparent box door 501, the second disinfection lamp 506 disinfects the inside of the electrostatic spinning device 5, the manufacturing process of the electrostatic spinning outer membrane is in an aseptic environment, and after the manufacturing of the electrostatic spinning outer membrane is finished, the second disinfection lamp 506 still works for a period of time to continuously disinfect related components for manufacturing the electrostatic spinning outer membrane.

Further, the receiving screen 601 is installed to the inside diapire of fixed plate 6, and receive screen 601 and high voltage power supply 502 and pass through wire electric connection, the outer wall of fixed plate 6 runs through and installs the carriage release lever 602, the outer wall of carriage release lever 602 encircles and installs first spring, when changing receiving screen 601, outside pulling carriage release lever 602, the inside of receiving screen 601 is left to the one end of carriage release lever 602, take off used receiving screen 601, change new receiving screen 601, loosen carriage release lever 602, the spring drives the inside removal of carriage release lever 602, carriage release lever 602 fixes receiving screen 601, realize conveniently changing the function of receiving screen 601.

The working principle is that firstly, a mixed solution is prepared after a solution A and a solution B are mixed, a cross-linking agent and thrombin are added into the mixed solution in a container barrel 408 for stirring, specifically, a driving motor 402 drives a screw rod 403 to rotate, the screw rod 403 converts the self rotary motion into the linear motion of a sliding plate, the sliding plate drives a movable sleeve 404 to move downwards, the movable sleeve 404 drives a connecting rod and a supporting disc to move downwards, when the movable sleeve 404 touches the top of a first pressure sensor 407, the first pressure sensor 407 starts a rotary motor 405, the rotary motor 405 drives a stirring rod 406 to rotate, the stirring rod 406 stirs the mixed solution in the container barrel 408, the reaction rate is accelerated, the preparation time of hydrogel is shortened, the preparation time is shortened, then when electrostatic spinning solution is supplemented into an emitter 503, a hydraulic push rod 504 drives a piston to move backwards, a floating plate 704 in a clamping groove drives a small column to move downwards, stopper 705 plays limiting displacement to the small circle post, prevent that the small circle post continues to descend, the top of small circle post is not extruding second pressure sensor 703, second pressure sensor 703 starts electric valve 701, electric valve 701 opens, the electrostatic spinning liquid in the liquid reserve tank 7 passes through the transfer line and flows in emitter 503, it rises along with the rising of liquid level to float board 704, inside until floating board 704 reenters the draw-in groove, small circle post upward movement extrudees second pressure sensor 703, second pressure sensor 703 senses pressure variation and closes electric valve 701, accomplish automatic replenishment electrostatic spinning liquid to the emitter through electric valve 701 and second pressure sensor 703, realize the automatic function of replenishing electrostatic spinning liquid.

As shown in fig. 10-13, further, a bearing mechanism 3 for bearing the measuring cylinder 105 is disposed in the placing cylinder 104, the bearing mechanism 3 includes a second supporting plate 301, a supporting cylinder 302, a second spring 303, and an intermediate bearing plate 304, the supporting cylinder 302 is disposed on the second supporting plate 301, a spring groove 305 is disposed in the second supporting plate 301, the second spring 303 is disposed in the spring groove 305, the intermediate bearing plate 304 is disposed on the second spring 303, and a plurality of first hinge seats 306 are disposed around the second spring 303, the first hinge seats 306 are hinged to the intermediate bearing plate 304 through first hinge rods 307, second hinge seats 308 corresponding to the first hinge seats 306 are disposed on the inner wall of the supporting cylinder 302, a vertical guide groove 309 is disposed on the inner wall of the supporting cylinder 302, the second hinge seats 308 are located in the vertical guide groove 309, and the second hinge seats 308 are hinged to the first hinge seats 306 through second rods 310, the upper end of the supporting cylinder 302 is provided with a top plate 311, the middle bearing plate 304 is provided with an upper fixing column 312, the upper end of the upper fixing column 312 extends out of the top plate 311, the measuring cylinder 105 is arranged on the upper fixing column 312, the upper fixing column 312 is provided with a fixing groove corresponding to the measuring cylinder 105, the second hinge seat 308 is connected with a third hinge seat 314 through a third hinge rod 313, the third hinge seat 314 is located at the bottom of the top plate 311, the third hinge seat 314 is provided with a propping seat 315 for propping against the upper fixing column 312, an inner vertical groove 316 is arranged in the wall of the supporting cylinder 302, the inner vertical groove 316 is located at the inner side of the vertical guide groove 309, the bottom of the inner vertical groove 316 is provided with a third spring 317, the third spring 317 is provided with a vertical support rod 318, the second hinge seat 308 is provided with a plurality of wedge blocks 319, the vertical strut 318 is provided with a wedge rod 320 corresponding to the wedge block 319, and the upper end of the vertical strut 318 is provided with a top pillar 321, and the top pillar 321 is connected with the top plate 311.

In use, the measuring cylinder 105 can be inserted into the fixing slot (not shown) of the upper fixing column 312 by the mechanical arm mechanism 106 (as shown in fig. 14) on the first support plate 1, and then the measuring cylinder 105 is pressed downward, so that the middle support plate 304 moves toward and approaches the second support plate 301, and then the middle support plate 304 presses the plurality of first hinge rods 307 around the middle support plate and moves the first hinge rods 307 toward the horizontal position in an inclined state, so that the plurality of first hinge seats 306 on the second support plate 301 also move outward, and the first hinge seats 306 drive the second hinge seats 308 to move upward along the inner wall of the support cylinder 302 through the second hinge rods 310, and then the second hinge seats 308 drive the third hinge seats 314 to move along the bottom of the top plate 311 through the third hinge rods 313, and the third hinge seats 314 approach the side of the upper fixing column 312, so that the outer surface of the upper fixing column 312 is pressed against and limited by the pressing seats 315 on the third hinge seats 313, thereby causing the upper fixing post 312 to be caught, causing the second spring 303 to contract downward when the middle carrier plate 304 is pressed, the middle carrier plate 301 is then caused to have a pulling force on the first hinge lever 307 by the second spring 303, so that the first hinge base 306 tends to move toward the second spring 303, and the second hinge base 308 is driven to move downward, since the wedge block 319 is installed on the second hinge seat 308, and the wedge block 319 and the wedge rod 320 can be inserted into each other for limiting, so that the wedge 319 is blocked, and so that the second hinge seat 308 is also restrained, so that the abutting base 315 can continuously abut against the upper fixing column 312 to ensure that the measuring cylinder 105 cannot move downwards after being clamped by the top plate 311, under the action of the second spring 303, the middle bearing plate 301 can stably support the upper fixing column 312, so that the phenomenon of deflection is prevented;

when the measuring cylinder 105 needs to be replaced, the mechanical arm mechanism 106 presses the top plate 311 downwards, so that the top column 321 also moves downwards, and further drives the vertical support rod 318 in the inner vertical groove 316 to move downwards to compress the third spring 317, so that the wedge rod 320 moves into the inner vertical groove 316 of the support cylinder 302 again, the wedge block 319 and the wedge rod 320 are separated from each other, the second hinge seat 308 can move downwards on the inner wall of the support cylinder 302, so that the third hinge seat 314 moves towards the support cylinder 302 and is separated from the upper fixing column 312, so that the measuring cylinder 105 is ejected upwards by the second spring 303, the middle bearing plate 304 and the upper fixing column 312, then the mechanical arm mechanism 106 can clamp the measuring cylinder 105 and then move above the guide pipe 203, and polyethylene glycol amino or polyethylene glycol-succinimidyl ester in the measuring cylinder 105 is added into the beaker 206 through the guide pipe 203, therefore, the process is more automatic, manual operation is reduced, and efficiency is improved.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; may be mechanically coupled, may be electrically coupled or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

While embodiments of the invention have been disclosed above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.

Claims

1. A method for preparing a hemostatic material for war wounds, the hemostatic material comprising an electrospun outer membrane (100) and a natural polysaccharide hydrogel (200) for application to a surface of the electrospun outer membrane (100) to rapidly gel with the electrospun outer membrane (100), the method comprising:

1) preparing a preparation material, namely selecting polyethylene glycol amino or polyethylene glycol-succinimide ester as the preparation material of the electrostatic spinning outer membrane (100); selecting a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle as preparation materials of the hydrogel (200);

2) preparing an electrostatic spinning outer membrane (1), and preparing the polyethylene glycol amino or polyethylene glycol-succinimide ester in the step 1) into electrostatic spinning solution; then, by means of an electrostatic spinning technology, electrostatic spinning is carried out under a received electric field, the fibers are gathered into a plurality of groups of first fiber bundles (300), the first fiber bundles (300) are mutually staggered to form a plurality of groups of second fiber bundles (400) of grids and membrane fibers (500) positioned among the grids, and the electrostatic spinning outer membrane (100) is prepared;

3) preparing a natural polysaccharide hydrogel (200), firstly obtaining a chitosan derivative, an aldehyde-based natural polysaccharide and a modified block copolymer micelle respectively; then respectively preparing a solution A containing chitosan derivatives and a solution B containing aldehyde-based natural polysaccharides, modified block copolymer micelles and photoinitiators; and finally, mixing the solution A and the solution B, adding a cross-linking agent and thrombin, and mixing and reacting at room temperature to obtain the natural hydrogel (200).

2. A device for preparing a hemostatic material for war wounds, comprising: the electrostatic spinning device comprises a first supporting plate (1) and an electrostatic spinning device (5) arranged on the first supporting plate (1), wherein the electrostatic spinning device (5) is used for preparing an electrostatic spinning outer membrane (100), the electrostatic spinning device (5) is positioned on the back face of a fastening mechanism, a fixing plate (6) is arranged on the inner bottom wall of the electrostatic spinning device (5), a first connecting rod is arranged on the inner front wall of the electrostatic spinning device (5), a liquid storage tank (7) is arranged at one end of the first connecting rod, a transverse seat plate is arranged on the inner bottom wall of the electrostatic spinning device (5) and positioned on one side of the fixing plate (6), an emitter (503) is arranged at the top of the transverse seat plate, a liquid conveying pipe penetrates through the bottom of the liquid storage tank (7), one end of the liquid conveying pipe penetrates through the outer wall of the emitter (503), an electric valve (701) is arranged on the outer wall of the liquid conveying pipe, and a clamping groove is formed in the inner wall of the emitter (503), the top of transmitter (503) is provided with notch plate (702), and the top of notch plate (702) is provided with second pressure sensor (703), and the bottom of notch plate (702) is equipped with the round hole, and the top of transmitter (503) is run through and is provided with little cylinder, and the one end of little cylinder extends into the inside of draw-in groove, and the outer wall of little cylinder is provided with stopper (705), and the bottom of little cylinder is provided with floating board (704).

3. The preparation equipment of hemostatic material for war wounds according to claim 2, characterized in that the top of the first support plate (1) is provided with a drive box (4), an antibacterial cover (41), the antibacterial cover (41) is located on the front of the electrostatic spinner (5), the inner wall of the antibacterial cover (41) is provided with a first disinfection lamp (42), the drive box (4) is located in the antibacterial cover (41), the top of the drive box (4) is provided with a support cylinder (401), the inner bottom wall of the drive box (4) is provided with a drive motor (402), the output end of the drive motor (402) is provided with a lead screw (403), one end of the lead screw (403) extends into the inside of the support cylinder (401), the outer wall of the support cylinder (401) is provided with a chute, the outer wall of the lead screw (403) is provided with a slide plate, and one end of the slide plate extends through the inside of the chute, the outer wall of slide is provided with and removes cover (404), the outer wall of removing cover (404) is provided with the second connecting rod, the one end of second connecting rod is provided with the support disc, the top of supporting the disc is provided with rotating electrical machines (405), the output of rotating electrical machines (405) is provided with stirring rod (406), the outer wall of support cylinder (401) is provided with first pressure sensor (407), the below of stirring rod (406) is provided with a container section of thick bamboo (408).

4. The preparation equipment of the hemostatic material for war wounds as claimed in claim 2, wherein a transparent box door (501) is installed on the outer wall of the electrostatic spinning device (5), a second disinfection lamp (506) is installed on the inner top wall of the electrostatic spinning device (5), a high voltage power supply (502) is installed on the inner bottom wall of the base, a hydraulic push rod (504) is installed on the inner wall of the electrostatic spinning device (5), a movable piston is installed at one end of the hydraulic push rod (504), a liquid spraying port (505) is installed at the top of the emitter (503) in a penetrating manner, and the liquid spraying port (505) is electrically connected with the high voltage power supply (502) through a conducting wire.

5. The preparation equipment of hemostatic materials for war wounds according to claim 2, characterized in that the inner bottom wall of the fixing plate (6) is provided with a receiving screen (601), the receiving screen (601) is electrically connected with the high voltage power supply (502) through a wire, the outer wall of the fixing plate (6) is provided with a moving rod (602) in a penetrating manner, and the outer wall of the moving rod (602) is provided with a first spring in a surrounding manner.

6. The apparatus for preparing hemostatic material for war wounds of claim 2, the top of the first supporting plate (1) is provided with a fixed cylinder (2), the top of the first supporting plate (1) is provided with a connecting column (201), the connecting column (201) is positioned on the back of the fixed cylinder (2), the outer wall of the connecting column (201) is provided with a sliding sleeve (202), the outer wall of the connecting column (201) is provided with a mounting block (207), the mounting block (207) is positioned below the sliding sleeve (202), the outer wall of the sliding sleeve (202) is provided with a third connecting rod (204), one end of the third connecting rod (204) is provided with a connecting disc (205), the inner wall of the fixed cylinder (2) is provided with a beaker (206), the top of the connecting disc (205) is provided with a guide pipe (203) in a penetrating manner, and one end of the guide pipe (203) extends into the beaker (206).

7. The device for preparing hemostatic material for war wounds according to claim 2, characterized in that the fastening means comprises a transverse electric rod (101), a rubber block (102), a vertical rod (103), wherein the fastening means is provided on both sides of the container barrel (408), the vertical rod (103) is disposed on the first support plate, the transverse electric rod is horizontally and rotatably disposed on the vertical rod (103), and the rubber block (102) is disposed on one end of the transverse electric rod (101) facing the container barrel (408) and abuts against the container barrel (408).

8. The apparatus for preparing hemostatic material for war wounds according to claim 2, characterized in that a placing cylinder (104) is provided on top of the first support plate (1), and a measuring cylinder (105) is provided in the placing cylinder (104).

9. The apparatus for preparing hemostatic material for war wounds according to claim 8, characterized in that a carrying mechanism (3) for carrying the measuring cylinder (105) is provided in the placement cylinder (104), the carrying mechanism (3) comprises a second support plate (301), a support cylinder (302), a second spring (303), an intermediate carrying plate (304), the support cylinder (302) is disposed on the second support plate (301), a spring groove (305) is provided in the second support plate (301), the second spring (303) is disposed in the spring groove (305), the intermediate carrying plate (304) is disposed on the second spring (303), and a plurality of first hinge seats (306) are provided around the second spring (303), the first seats (306) are hinged to the intermediate carrying plate (304) by a first hinge rod (307), a second hinge seat (308) corresponding to the first hinge seat (306) is arranged on the inner wall of the support cylinder (302), a vertical guide groove (309) is arranged on the inner wall of the support cylinder (302), the second hinge seat (308) is located in the vertical guide groove (309), the second hinge seat (308) is hinged to the first hinge seat (306) through a second hinge rod (310), a top plate (311) is arranged at the upper end of the support cylinder (302), an upper fixing column (312) is arranged on the middle bearing plate (304), the top plate (311) extends out from the upper end of the upper fixing column (312), the measuring cylinder (105) is arranged on the upper fixing column (312), a fixing groove corresponding to the measuring cylinder (105) is arranged on the upper fixing column (312), and a third hinge seat (314) is connected to the second hinge seat (308) through a third hinge rod (313), the third hinge seat (314) is located at the bottom of the top plate (311), a propping seat (315) for propping against the upper fixing column (312) is arranged on the third hinge seat (314), an inner vertical groove (316) is arranged in the wall of the supporting cylinder (302), the inner vertical groove (316) is located on the inner side of the vertical guide groove (309), a third spring (317) is arranged at the bottom of the inner vertical groove (316), the third spring (317) is provided with a vertical supporting rod (318), a plurality of wedge-shaped blocks (319) are arranged on the second hinge seat (308), wedge-shaped rods (320) corresponding to the wedge-shaped blocks (319) are arranged on the vertical supporting rod (318), a top column (321) is arranged at the upper end of the vertical supporting rod (318), and the top column (321) is connected with the top plate (311).