CN113549585A - Salt-tolerant heterotrophic nitrification aerobic denitrification bacterium and application thereof - Google Patents
Salt-tolerant heterotrophic nitrification aerobic denitrification bacterium and application thereof Download PDFInfo
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- 241000588749 Klebsiella oxytoca Species 0.000 claims abstract description 26
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- 238000004321 preservation Methods 0.000 claims abstract description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 239000002068 microbial inoculum Substances 0.000 claims 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 abstract description 10
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- 238000000034 method Methods 0.000 description 9
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- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 2
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
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- 239000012138 yeast extract Substances 0.000 description 2
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- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
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- 230000004544 DNA amplification Effects 0.000 description 1
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- 241000588748 Klebsiella Species 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
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- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
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- 239000001632 sodium acetate Substances 0.000 description 1
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- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
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- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a salt-tolerant heterotrophic nitrification aerobic denitrification strain, which belongs to the technical field of biology and has a preservation number of GDMCC NO. 61505. The invention separates klebsiella oxytoca (Klebsiella oxytoca) from the culture wastewaterKlebsiella oxytoca) YZ-12, the strain has the salt-tolerant, heterotrophic nitrification and aerobic denitrification capabilities, can still grow and partially denitrify when the salinity is 30g/L, and has the denitrification rate of more than 97 percent for ammonia nitrogen wastewater with the concentration of less than 150mg/L and nitrate nitrogen wastewater with the concentration of less than 500 mg/L. The strain has potential application prospect in sewage treatment.
Description
Technical Field
The invention relates to a salt-tolerant heterotrophic nitrification aerobic denitrification bacterium and application thereof, belonging to the technical field of environmental microorganisms.
Background
With the increasingly prominent problem of water eutrophication, the prevention and treatment of nitrogen pollution are more and more paid attention by people. The existing sewage denitrification treatment method mainly comprises a chemical method, a biochemical method and a biophysical-physicochemical combined process, wherein the biological denitrification is a main means for sewage denitrification by the characteristics of economy, high efficiency and no residual pollution. However, the different processes of the traditional biological denitrification and denitrification require to be carried out in two reactors, so that the construction cost is high, and each step needs to be respectively added with a carbon source, acid and alkali regulation and the like.
In recent years, with the discovery of strains with heterotrophic nitrification-aerobic denitrification characteristics, the research and development of sewage denitrification technology by using heterotrophic nitrification-aerobic denitrification bacteria is increasingly a research hotspot. Compared with the traditional nitrifying microorganisms, the heterotrophic nitrification aerobic denitrifying bacteria have higher cell growth rate, realize synchronous completion of nitrification and denitrification reactions in the same reactor, and simultaneously convert ammonia nitrogen, nitrate nitrogen and nitrite nitrogen into nitrogen-containing gas. In addition, acid and alkali respectively generated in the nitrification process and the denitrification process can be mutually neutralized, so that the acid-alkali balance of the water body is relatively maintained, and the pH adjustment cost is reduced. Some special heterotrophic nitrification aerobic denitrification bacteria even have the characteristics of high ammonia nitrogen tolerance, high salinity tolerance, low temperature tolerance and low C/N tolerance. These advantages make heterotrophic nitrification aerobic denitrification bacteria become a research hotspot for sewage denitrification treatment.
Disclosure of Invention
The invention provides a salt-tolerant heterotrophic nitrification aerobic denitrification strain which can be applied to the field of sewage treatment.
Klebsiella oxytoca (Klebsiella oxytoca, etc.) provided by the present inventionKlebsiella oxytoca) YZ-12, deposited at 21.7.2021 in the Guangdong province culture Collection with the deposit number GDMCC NO. 61505.
The screened Klebsiella oxytoca YZ-12 is applied to sewage treatment.
The invention also provides a method for carrying out sewage denitrification treatment by applying the Klebsiella oxytoca YZ-12, which comprises the following steps:
(1) activating acid-producing Klebsiella bacteria YZ-12; specifically, Klebsiella oxytoca YZ-12 can be inoculated into a solid culture medium and cultured for 24 hours at the temperature of 28-35 ℃ to prepare an activated strain; the solid culture medium is obtained by adding 15-20 g/L agar powder into an LB culture medium.
(2) Preparing a seed solution; specifically, inoculating the activated strain prepared in the step (1) into a seed culture medium, and culturing at 28-35 ℃ and 120-200 r/min for 12-16 h to obtain a seed solution; the seed medium may be: 1g/L tryptone, 0.5g/L yeast extract, 0.1g/L potassium nitrate and pH 7.0-7.5.
(3) Carrying out denitrification treatment on the sewage; specifically, inoculating the seed solution obtained in the step (2) into the sewage to be treated according to the volume ratio of 5%, adding sucrose as a carbon source, adjusting the C/N to be 10-20 and the pH to be 6-9, culturing for 24-48 h at the temperature of 28-35 ℃ and under the condition of 120-200 r/min, and taking a water sample to detect the denitrification effect of the strain.
The Klebsiella oxytoca YZ-12 has certain high salt tolerance, and can be cultured in a denitrification culture medium containing 30g/L sodium chloride for 48h, with the strain number reaching 108cell/mL。
The invention has the beneficial effects that:
the Klebsiella oxytoca YZ-12 provided by the invention has characteristics which are very suitable for sewage denitrification treatment. The living bacteria have salt tolerance, good nitrification capacity when the salinity is 10g/L, and good denitrification capacity when the salinity is less than or equal to 30 g/L; the strain also has a good acid-base adaptation range, and has a good denitrification effect within a pH range of 6-9; the most suitable carbon source is sucrose; the optimum C/N range for denitrification is 10-20.
Drawings
FIG. 1 bacterial strain YZ-12 plate colony morphology.
FIG. 2 PCR gel electrophoresis image of strain YZ-12.
FIG. 3 phylogenetic tree of strain YZ-12.
FIG. 4 the effect of different carbon sources on the denitrification effect of strain YZ-12.
FIG. 5 Effect of different initial nitrogen solubility on the denitrification effect of strain YZ-12.
FIG. 6 shows the effect of different carbon nitrogen ratios on the denitrification effect of the strain YZ-12.
FIG. 7 is a graph showing the effect of different pH values on the denitrification effect of the strain YZ-12.
FIG. 8 the effect of different salinity on the denitrification effect of strain YZ-12.
FIG. 9 shows the denitrification effect of the strain YZ-12 on the culture wastewater.
Detailed Description
The following examples further illustrate the invention. The present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a process are given, but the scope of the present invention is not limited to the following embodiments.
Example 1
Screening, identifying and storing Klebsiella oxytoca YZ-12
(1) Screening of Klebsiella oxytoca YZ-12
Adding a water sample of an aerobic pool of a sewage treatment station of a farm into a sterile primary screening culture medium according to the inoculation amount of 10%, culturing for 24 hours at the constant temperature of 30 ℃ and a shaking table at 120rpm, and continuously carrying out passage for 3 times. Diluting the culture solution obtained by continuous culture for 3 times with sterile water 10 times-5、10-6、10-7The method comprises the following steps of coating the single colony on a primary screening solid culture medium, culturing at the constant temperature of 30 ℃ for 1-2 days, selecting the blue single colony on a colony area after the single colony grows on a flat plate, scribing on the primary screening solid culture medium, repeatedly selecting the single colony to scribe again after the single colony grows on the flat plate, finally scribing on an LB solid culture medium test tube inclined plane, and storing in a refrigerator at 4 ℃.
The formula of the primary screening culture medium is as follows: c4H4Na2O4·6H2O 8.5g/L，KNO3 1g/L，MgSO4·7H2O 1g/L，KH2PO41g/L，CaCl2·2H2O 0.2g/L，FeSO4·7H2O0.05 g/L, 1% bromothymol blue 1mL/L, pH 7.0.
The formula of the primary screening solid culture medium is that agar is added by 20g/L on the basis of the formula of the primary screening culture medium.
(2) Identification of Klebsiella oxytoca YZ-12
As shown in the figure I, the bacterial colony of the strain YZ-12 on an LB medium plate is round, white, smooth in surface, viscous, elastic and capable of being pulled into a filamentous shape. The thallus is rod-shaped under microscope.
The selected strains were subjected to 16S rDNA sequencing. After PCR amplification, the obtained 16S rDNA sequence is compared on NCBI and then is subjected to phylogenetic tree construction (see figure III), and the strain is determined to be Klebsiella oxytoca YZ-12.
(3) Preservation of Klebsiella oxytoca YZ-12
Selecting a Klebsiella oxytoca YZ-12 strain, inoculating the Klebsiella oxytoca YZ-12 strain into an LB culture medium, culturing at 30 ℃ and 120rpm for 12-24 h at constant temperature, transferring 0.6mL of culture solution into a 40% glycerol storage tube filled with 0.6mL of culture solution, and performing cryopreservation at-20 ℃. The strain is preserved in Guangdong province microorganism culture collection center at 2021, 7 months and 21 days, and the preservation number is GDMCC NO. 61505.
Example 2
Denitrification effect of Klebsiella oxytoca YZ-12 under different conditions
(1) Detection of influence of carbon source on denitrification effect of Klebsiella oxytoca YZ-12
Preparing a seed solution: and inoculating Klebsiella oxytoca YZ-12 stored in a refrigerator at 4 ℃ in the embodiment 1 into a 250mL conical flask filled with 50mL of seed culture medium, and culturing at 28-35 ℃ under the condition of 120-200 r/min for 12-16 h to obtain a seed solution.
And (3) denitrification effect detection: preparing ammonia nitrogen culture medium and nitrate nitrogen culture medium by using glucose, sucrose, sodium acetate, methanol and sodium succinate as unique carbon sources, and preparing NH4 +-N concentration of 350mg/L, NO3 -The concentration of-N is 415mg/L, seed liquid is added according to the inoculation amount of 5 percent, the mixture is cultured for 72 hours under the conditions of 28-35 ℃ and 120-200 r/min, sampling is carried out every 24 hours, centrifugation is carried out at 8000rpm, supernatant is taken, and NH is detected by a nano-reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Wherein the sucrose is used as a carbon source and NH is contained in an ammonia nitrogen culture medium4 +The removal rate of-N is 42.86 percent, and NO is contained in the nitrate nitrogen culture medium by taking cane sugar as a carbon source3 -The removal rate of-N is 99.84%, and neither the ammonia nitrogen culture medium nor the nitrate nitrogen culture medium has NO2 --N accumulation.
(2) Detection of influence of nitrogen source concentration on denitrification effect of Klebsiella oxytoca YZ-12
Preparation of NH4 +An N concentration gradient of 50, 100, 150, 200, 250 mg-L，NO3 -Adding an ammonia nitrogen culture medium and a nitrate nitrogen culture medium of sucrose with the N concentration of 400, 450, 500, 550, 600mg/L and 9.8g/L into the seed solution according to the inoculation amount of 5%, culturing for 72h under the conditions of 28-35 ℃ and 120-200 r/min, sampling every 24h, centrifuging at 8000rpm, taking supernatant, and detecting NH by using a Nashin reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Wherein NH4 +NH at a N concentration of 50, 100mg/L4 +The removal rate of-N is more than 99 percent, NO3 -NO at N concentration of 400, 450mg/L3 -The removal rate of-N is 99%, and NO NO is generated2 --N accumulation.
(3) Detection of influence of C/N on denitrification effect of Klebsiella oxytoca YZ-12
Fixation of NH4 +N concentration of 100mg/L, NO3 -The concentration of N is 415mg/L, and the C/N is adjusted to be 2.5, 5, 10, 15 and 20. Adding the seed solution according to the inoculation amount of 5%, culturing for 72h under the conditions of 28-35 ℃ and 120-200 r/min, sampling every 24h, centrifuging at 8000rpm, taking supernatant, and detecting NH by using a nano-grade reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Wherein when C/N is 10, 15, 20, NH4 +-N and NO3 -The removal rate of-N is more than 99 percent, and NO NO is generated2 --N accumulation.
(4) Detection of influence of pH on effect of Klebsiella oxytoca YZ-12 nitrogen
Configuration of NH4 +N concentration of 100mg/L, NO3 -Ammoniacal nitrogen medium, nitrate nitrogen medium with N concentration 415mg/L and C/N10, adjusted to pH =5, pH =6, pH =7, pH =8, pH =9 with 1mol/L hydrochloric acid solution and 1mol/L sodium hydroxide solution, respectively. Adding the seed solution according to the inoculation amount of 5%, culturing for 72h under the conditions of 28-35 ℃ and 120-200 r/min, sampling every 24h, centrifuging at 8000rpm, taking supernatant, and detecting NH by using a nano-grade reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Wherein NH when the pH is 7, 8 or 94 +-N and NO3 -The removal rate of-N is more than 99 percent, and NO NO is generated2 --N accumulation.
(5) Detection of influence of salinity on denitrification effect of Klebsiella oxytoca YZ-12
Configuration of NH4 +N concentration of 100mg/L, NO3 -An ammonia nitrogen culture medium and a nitrate nitrogen culture medium with the N concentration of 415mg/L and the C/N of 10 are added with NaOH with the mass ratio of 1%, 2%, 3%, 4% and 5% respectively. Adding the seed solution according to the inoculation amount of 5%, culturing for 72h under the conditions of 28-35 ℃ and 120-200 r/min, sampling every 24h, centrifuging at 8000rpm, taking supernatant, and detecting NH by using a nano-grade reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Wherein NH when the salinity is 1%4 +-N and NO3 -The N removal rate is more than 99 percent; NH when salinity is 2%4 +the-N removal rate was 66.7%, NO3 --N removal 99.9%; NH when salinity is 3%4 +-N removal of 23.0%, NO3 -The removal rate of-N is 99.7%, and NO NO is generated2 --N accumulation.
The formula of the culture medium comprises:
seed culture medium: tryptone 1g/L, yeast extract 0.5g/L, KNO30.1g/L and a pH value of 7.0.
An ammonia nitrogen culture medium: c4H4Na2O4·6H2O 11g/L，Na2HPO4·12H2O 6.7g/L，KH2PO4 1g/L，NH4Cl 1.5g/L，MgSO4·7H20.1g/L of O, 2mL of trace element solution and a pH value of 7.0-7.3.
Nitrate nitrogen culture medium: sucrose 5g/L, Na2HPO4·12H2O 7.9g/L，KH2PO4 1.5g/L，MgSO4·7H2O 0.1g/L，KNO3 3g/L, 2mL of trace element solution and a pH value of 7.0-7.3.
Trace elements: EDTA 50g/L, CaCl2·2H2O 7.28g/L，FeSO4·7H2O 5g/L，ZnSO4·7H2O 3.92g/L，MnCl2·4H2O 2.06g/L，CoCl2·6H2O 1.61g/L，CuSO4·5H2O 1.57g/L，(NH4)6Mo7O24·4H2O1.1 g/L, pH 6.0.
Example 3
Klebsiella oxytoca YZ-12 denitrification treatment effect on aquaculture wastewater
Adjusting the C/N of the culture wastewater to 10 by using sucrose, adding the seed solution of the example 2 according to the inoculation amount of 5%, culturing for 72h under the conditions of 28-35 ℃ and 120-200 r/min, sampling every 24h, centrifuging at 8000rpm, taking supernatant, and detecting NH by using a Nashin reagent spectrophotometry4 +-N concentration, detection of NO by ion chromatography3 --N concentration. Fruiting aquaculture wastewater NH4 +-N removal 95.15%, NO3 -the-N removal rate was 99.76%.
The water quality condition of the aquaculture wastewater is as follows: the pH value is 7.8, the COD value is 2031mg/L, the ammonia nitrogen is 362mg/L, the nitrate nitrogen is 23.8mg/L, and the nitrite nitrogen is 0.167 mg/L.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Sequence listing
<110> Jiangxi Zhongjiang environmental protection group Limited
<120> one strain of salt-tolerant heterotrophic nitrification aerobic denitrification bacteria and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1406
<212> DNA
<213> 16S rRNA Gene amplification product ()
<400> 1
cagtcgaacg gtagcacaga gagcttgctc tcgggtgacg agtggcggac gggtgagtaa 60
tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat 120
aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcagatg tgcccagatg 180
ggattagcta gtaggtgggg taacggctca cctaggcgac gatccctagc tggtctgaga 240
ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag gcagcagtgg 300
ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg aagaaggcct 360
tcgggttgta aagtactttc agcggggagg aaggcgataa ggttaataac cttgtcgatt 420
gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg taatacggag 480
ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac gcaggcggtc tgtcaagtcg 540
gatgtgaaat ccccgggctc aacctgggaa ctgcattcga aactggcagg ctggagtctt 600
gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc 660
ggtggcgaag gcggccccct ggacaaagac tgacgctcag gtgcgaaagc gtggggagca 720
aacaggatta gataccctgg tagtccacgc tgtaaacgat gtcgacttgg aggttgttcc 780
cttgaggagt ggcttccgga gctaacgcgt taagtcgacc gcctggggag tacggccgca 840
aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat 900
tcgatgcaac gcgaagaacc ttacctactc ttgacatcca gagaacttag cagagatgct 960
ttggtgcctt cgggaactct gagacaggtg ctgcatggct gtcgtcagct cgtgttgtga 1020
aatgttgggt taagtcccgc aacgagcgca acccttatcc tttgttgcca gcgattcggt 1080
cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc 1140
atcatggccc ttacgagtag ggctacacac gtgctacaat ggcatataca aagagaagcg 1200
acctcgcgag agcaagcgga cctcataaag tatgtcgtag tccggattgg agtctgcaac 1260
tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt 1320
tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt 1380
agcttaacct tcgggagggc gctacc 1406
Claims (3)
1. A strain of salt-tolerant heterotrophic nitrification aerobic denitrification bacteria is characterized in that: the strain is Klebsiella oxytoca (A), (B), (C) and (C)Klebsiella oxytoca) YZ-12, the strain is preserved in Guangdong province microorganism strain preservation center, and the strain preservation number is GDMCC NO. 61505.
2. The salt-tolerant heterotrophic nitrification-aerobic denitrification bacterial strain of claim 1, wherein: has the salt-tolerant and heterotrophic nitrification-aerobic denitrification capability.
3. The application of the salt-tolerant heterotrophic nitrification aerobic denitrification bacterial strain as claimed in claim 1 in sewage treatment as a sewage treatment microbial inoculum.
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