CN113546102A

CN113546102A - Traditional Chinese medicine composition for promoting myocardial regeneration, preparation method and application thereof

Info

Publication number: CN113546102A
Application number: CN202010331462.7A
Authority: CN
Inventors: 李连滋; 林铮; 蔡蕙冰; 陈美惠
Original assignee: Jianyu Biotechnology Co ltd
Current assignee: Jianyu Biotechnology Co ltd
Priority date: 2020-04-24
Filing date: 2020-04-24
Publication date: 2021-10-26

Abstract

The invention provides a traditional Chinese medicine composition for promoting myocardial regeneration, which comprises at least two of the following traditional Chinese medicines in a specific ratio: radix Codonopsis, Saviae Miltiorrhizae radix and radix Panacis Quinquefolii. The invention also provides a preparation method of the traditional Chinese medicine composition for promoting myocardial regeneration, a medicine containing the traditional Chinese medicine composition and application of the medicine for preparing the medicine for promoting myocardial regeneration.

Description

Traditional Chinese medicine composition for promoting myocardial regeneration, preparation method and application thereof

Technical Field

The invention relates to a composition, a preparation method and application thereof, in particular to a traditional Chinese medicine composition for promoting myocardial regeneration, a preparation method and application thereof for promoting myocardial regeneration.

Background

Myocardial Infarction (MI) is a clinically common acute heart disease caused by sudden interruption of blood circulation in a portion of the myocardium, which is damaged by the inability to obtain sufficient oxygen. It is generally believed that once a patient has suffered a myocardial infarction, necrotic myocardial cells cannot regenerate, but the damaged area of the myocardium can only be repaired by forming fibrous plaques, and the fibrous plaques formed after repair are rigid scar tissues and do not have the function of contraction, which can help the heart to maintain the structural integrity, but further affect the cardiac remodeling and cause dysfunction, and even cause symptoms such as heart failure, cardiac arrhythmia, cardiogenic shock and the like.

The current treatment method for myocardial infarction mainly comprises two modes of invasive treatment and drug administration; among them, the invasive treatments are balloon dilatation, stent placement, coronary bypass surgery, etc., and the therapeutic drugs are mainly thrombolytic drugs, calcium ion blockers, nitrates, angiotensin converting enzyme inhibitors, B-sympathetic blockers and narcotic analgesics, etc.

However, in either clinical or basic medical research fields, invasive or drug therapies developed to date have failed to promote the recovery of damaged myocardial tissue, are more likely to derive other side effects, and have no effective means for the damaged myocardial tissue other than waiting for it to recover itself.

Therefore, there is still a need for developing a technique for promoting myocardial regeneration and effectively curing myocardial infarction in clinical treatment or basic medical research.

Disclosure of Invention

In view of the defects of the prior art, the invention aims to provide a traditional Chinese medicine composition which can promote the regeneration and repair of myocardial cells, and further provides a non-chemosynthetic drug and a non-invasive treatment strategy for treating myocardial infarction.

To achieve the above objects, the present invention provides a Chinese medicinal composition for promoting myocardial regeneration, which comprises at least two selected from the group consisting of the following Chinese medicinal materials: radix Codonopsis, Saviae Miltiorrhizae radix and radix Panacis Quinquefolii; when the traditional Chinese medicine composition comprises codonopsis pilosula and salvia miltiorrhiza, wherein the dosage of the codonopsis pilosula is 40-60 weight percent and the dosage of the salvia miltiorrhiza is 40-60 weight percent based on the total weight of the traditional Chinese medicine composition; when the traditional Chinese medicine composition comprises the salvia miltiorrhiza and the American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the salvia miltiorrhiza is 40-60 weight percent, and the American ginseng is 40-60 weight percent; when the traditional Chinese medicine composition comprises codonopsis pilosula and American ginseng, wherein the dosage of the codonopsis pilosula is 40-60 weight percent and the dosage of the American ginseng is 40-60 weight percent based on the total weight of the traditional Chinese medicine composition; when the traditional Chinese medicine composition comprises radix codonopsis, salvia miltiorrhiza and American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the dosage of the radix codonopsis is 20-65 weight percent, the dosage of the salvia miltiorrhiza is 10-60 weight percent, and the dosage of the American ginseng is 10-55 weight percent.

The traditional Chinese medicine composition of the invention contains at least two of radix codonopsitis, salvia miltiorrhiza and American ginseng in a specific ratio, so that the traditional Chinese medicine composition has the efficacy of promoting the regeneration and repair of myocardial cells.

According to the present invention, "Codonopsis pilosula" is a dried root of Codonopsis pilosula (Franch.) Nannf.), Codonopsis pilosula (Codonopsis pilosula Nannf., var. modesta (Nannf.) L.T.Shen) or Codonopsis tangshen (Codonopsis tangshen Oliv) of Campanulaceae (Campanulaceae) plant; the Saviae Miltiorrhizae radix is dried root and rhizome of Salvia miltiorrhiza Bge (Salvia milirhizorrhiza Bge.) of Labiatae (Labiatae); "American ginseng" is the dried root of American ginseng (Panax quinquefolium L.) of Araliaceae (Araliaceae) plant.

Preferably, the Chinese medicinal composition comprises codonopsis pilosula and at least one of salvia miltiorrhiza and American ginseng; when the traditional Chinese medicine composition comprises codonopsis pilosula and salvia miltiorrhiza, wherein the dosage of the codonopsis pilosula is 45-55 weight percent and the dosage of the salvia miltiorrhiza is 45-55 weight percent based on the total weight of the traditional Chinese medicine composition; when the traditional Chinese medicine composition comprises codonopsis pilosula and American ginseng, wherein the dosage of the codonopsis pilosula is 45-55 weight percent and the dosage of the American ginseng is 45-55 weight percent based on the total weight of the traditional Chinese medicine composition; when the traditional Chinese medicine composition comprises radix codonopsis, salvia miltiorrhiza and American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the dosage of the radix codonopsis is 20-30 weight percent, the dosage of the salvia miltiorrhiza is 20-60 weight percent, and the dosage of the American ginseng is 10-55 weight percent.

In another embodiment of the present invention, the traditional Chinese medicine composition for promoting myocardial regeneration comprises: a first Chinese medicinal material selected from the group consisting of Codonopsis pilosula, Salvia miltiorrhiza, and Panax quinquefolium; a second Chinese medicinal material selected from the group consisting of radix Codonopsis, Saviae Miltiorrhizae radix and radix Panacis Quinquefolii, and the first and second Chinese medicinal materials have different components; based on the total weight of the traditional Chinese medicine composition, the dosage of the first traditional Chinese medicine is 40-60 weight percent, and the dosage of the second traditional Chinese medicine is 40-60 weight percent.

Preferably, the traditional Chinese medicine composition comprises 20 to 30 weight percent of codonopsis pilosula, 20 to 60 weight percent of salvia miltiorrhiza and 10 to 55 weight percent of American ginseng, based on the total weight of the traditional Chinese medicine composition. Preferably, the amount of the codonopsis pilosula is 20 to 30 weight percent, the amount of the salvia miltiorrhiza is 20 to 30 weight percent, and the amount of the American ginseng is 45 to 55 weight percent based on the total weight of the traditional Chinese medicine composition.

Preferably, the traditional Chinese medicine composition comprises codonopsis pilosula, salvia miltiorrhiza and American ginseng, wherein the dosage of the codonopsis pilosula is 60-65 weight percent, the dosage of the salvia miltiorrhiza is 10-60 weight percent and the dosage of the American ginseng is 10-40 weight percent based on the total weight of the traditional Chinese medicine composition. Preferably, the amount of the codonopsis pilosula is 60 to 65 weight percent, the amount of the salvia miltiorrhiza is 20 to 30 weight percent, and the amount of the American ginseng is 10 to 20 weight percent based on the total weight of the traditional Chinese medicine composition.

The invention also provides a preparation method of the traditional Chinese medicine composition for promoting myocardial regeneration, which comprises the following steps: step (a): extracting a Chinese medicinal composition to obtain an extractive solution; and a step (b): filtering the extract and drying to obtain the traditional Chinese medicine composition for promoting myocardial regeneration.

Preferably, in the step (b), the extraction step is carried out by heating water, and the water temperature is 80 ℃ to 90 ℃ and the time is 2 hours to 3 hours.

The invention also provides a traditional Chinese medicine preparation for promoting myocardial regeneration, which comprises the traditional Chinese medicine composition for promoting myocardial regeneration and a medically acceptable carrier.

The invention also provides an application of the traditional Chinese medicine composition for promoting myocardial regeneration in preparing a medicine for promoting myocardial regeneration, wherein the medicine comprises the traditional Chinese medicine composition for promoting myocardial regeneration and a medically acceptable carrier.

According to the present invention, the aforementioned "carrier" or "pharmaceutically acceptable carrier" refers to a pharmaceutical carrier conventionally selected in the field of medicine, such as filler, binder, diluent, excipient, acceptor or the like, which is well known to those skilled in the art of pharmaceutical manufacturing.

According to the present invention, the dosage of the above-mentioned Chinese medicinal preparation or medicine can be determined by the relevant medical personnel according to the common knowledge in the technical field according to the factors of the age, weight, health condition, disease progression and the like of the patients. The above-mentioned Chinese medicinal preparation or medicine can also be administered alone or together with other medicines, and the course of administration should be carried out according to the routine method in pharmacy by physicians or relevant persons.

In the specification, a range represented by "a small value to a large value" means a range from greater than or equal to the small value to less than or equal to the large value, if not specifically indicated. For example, "40 weight percent to 60 weight percent" means that it ranges from "greater than or equal to 40 weight percent to less than or equal to 60 weight percent".

Drawings

Fig. 1 is a graph showing the effects of the Chinese medicinal compositions of examples 1 to 11 on the post-traumatic repair of the myocardium of zebra fish.

Detailed Description

The following examples are presented to illustrate embodiments of the present invention, and those skilled in the art can readily appreciate the advantages and utilities of the present invention without departing from the spirit and scope of the present invention.

Example 1

Cutting 2.5 g of radix Codonopsis (produced in China mainland) and 2.5 g of Saviae Miltiorrhizae radix (produced in China mainland) into pieces with a length of about 5 cm, placing in 50 ml of water, heating until the water temperature reaches 80 deg.C, extracting for 2 hr, filtering out residual medicinal materials with filter paper with pore size of 6 μm to obtain an extractive solution, and freeze drying with freeze dryer to obtain 1.8368 g of Chinese medicinal composition. The components, amounts and weight percentages of the Chinese medicinal composition of example 1 are listed in Table 1.

Example 2

The procedure for preparing the chinese medicinal composition of example 2 is similar to that of example 1, except that example 2 is prepared by cutting 2.5 g of codonopsis pilosula and 2.5 g of panax quinquefolius (canada, origin) into a length of about 5 cm and putting them into 50 ml of water, and then 1.7874 g of the chinese medicinal composition is prepared according to the same procedure as example 1. The components, amounts and weight percentages of the Chinese medicinal composition of example 2 are listed in table 1.

Example 3

The procedure for preparing the chinese medicinal composition of example 3 is similar to that of example 1, except that in example 3, 2.5 g of salvia miltiorrhiza and 2.5 g of panax quinquefolius are cut into about 5 cm in length and put into 50 ml of water, and then 1.7144 g of the chinese medicinal composition is prepared according to the same procedure as in example 1. The components, amounts and weight percentages of the Chinese medicinal composition of example 3 are listed in table 1.

Example 4

Cutting 1.67 grams of codonopsis pilosula, 1.67 grams of salvia miltiorrhiza and 1.67 grams of American ginseng into about 5 centimeters in length, putting the cut pieces into 50 milliliters of water, heating the water until the water temperature reaches 80 ℃, extracting the water for 2 hours, filtering residual medicinal materials by using filter paper with the aperture of 6 mu m to obtain an extract, and freeze-drying the extract by using a freeze dryer to obtain 1.8725 grams of the traditional Chinese medicine composition. The components, amounts and weight percentages of the Chinese medicinal composition of example 4 are listed in table 1.

Example 5

The procedure for preparing the chinese medicinal composition of example 5 is similar to that of example 4, except that in example 5, 1 g of codonopsis pilosula, 2 g of salvia miltiorrhiza bunge and 1 g of panax quinquefolius are cut into about 5 cm in length and put into 40 ml of water, and then 1.2984 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 5 are listed in table 1.

Example 6

The procedure for preparing the chinese medicinal composition of example 6 is similar to that of example 4, except that in example 6, 1 g of codonopsis pilosula, 1 g of salvia miltiorrhiza bunge and 2 g of panax quinquefolius are cut into about 5 cm in length and put into 40 ml of water, and then 1.2325 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 6 are listed in table 1.

Example 7

The procedure for preparing the chinese medicinal composition of example 7 is similar to that of example 4, except that in example 7, 2 g of codonopsis pilosula, 1 g of salvia miltiorrhiza bunge and 0.5 g of panax quinquefolius are cut into about 5 cm in length and put into 35 ml of water, and then 1.1644 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 7 are listed in Table 1.

Example 8

The procedure for preparing the chinese medicinal composition of example 8 is similar to that of example 4, except that in example 8, 2 g of codonopsis pilosula, 0.5 g of salvia miltiorrhiza bunge and 1 g of panax quinquefolius are cut into about 5 cm in length and put into 35 ml of water, and then 1.1029 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 8 are listed in table 1.

Example 9

The procedure for preparing the chinese medicinal composition of example 9 is similar to that of example 4, except that in example 9, 1 g of codonopsis pilosula, 2 g of salvia miltiorrhiza bunge and 0.5 g of panax quinquefolius are cut into about 5 cm in length and put into 35 ml of water, and then 1.0907 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 9 are listed in table 1.

Example 10

The procedure for preparing the chinese medicinal composition of example 10 is similar to example 4, except that example 10 is prepared by cutting 2.5 g of codonopsis pilosula, 1.25 g of salvia miltiorrhiza bunge and 1.25 g of panax quinquefolius into about 5 cm in length and putting into 50 ml of water, and then 1.9172 g of the chinese medicinal composition is prepared according to the same procedure as example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 10 are listed in table 1.

Example 11

The procedure for preparing the chinese medicinal composition of example 11 is similar to that of example 4, except that in example 11, about 3 g of codonopsis pilosula, about 1.2 g of salvia miltiorrhiza bunge and about 0.8 g of panax quinquefolius are cut into about 5 cm in length and put into 50 ml of water, and then 2.2586 g of the chinese medicinal composition is prepared according to the same procedure as in example 4. The components, amounts and weight percentages of the Chinese medicinal composition of example 11 are listed in table 1.

Table 1: the components, amounts and weight percentages (wt%) of the Chinese medicinal compositions of examples 1 to 11

Numbering	Codonopsis pilosula	Root of red-rooted salvia	Radix Panacis Quinquefolii
				Example 1	2.5 g (50.0 wt%)	2.5 g (50.0 wt%)	--
Example 2	2.5 g (50.0 wt%)	--	2.5 g (50.0 wt%)
				Example 3	--	2.5 g (50.0 wt%)	2.5 g (50.0 wt%)
Example 4	1.67 gram (33.3 wt%)	1.67 gram (33.3 wt%)	1.67 gram (33.3 wt%)
				Example 5	1 gram (25.0 wt%)	2 gram (50.0 wt%)	1 gram (25.0 wt%)
Example 6	1 gram (25.0 wt%)	1 gram (25.0 wt%)	2 gram (50.0 wt%)
				Example 7	2 gram (57.1 wt%)	1 gram (28.6 wt%)	0.5 gram (14.3 wt%)
Example 8	2 gram (57.1 wt%)	0.5 gram (14.3 wt%)	1 gram (28.6 wt%)
				Example 9	1 gram (28.6 wt%)	2 gram (57.1 wt%)	0.5 gram (14.3 wt%)
Example 10	2.5 g (50.0 wt%)	1.25 gram (25.0 wt%)	1.25 gram (25.0 wt%)
				Example 11	3 gram (60.0 wt%)	1.2 gram (24.0 wt%)	0.8 gram (16.0 wt%)

Test example: evaluation of myocardial regeneration efficacy of zebra fish

After the zebrafish heart is frozen to cause about 30% of wounds, the injury can be repaired within 21 days without administration of the composition, so that adult zebrafish is used as an experimental animal for studying heart damage. After the heart of the zebra fish is damaged, the regeneration process can be divided into three stages, the myocardial cells proliferate in the initial stage, the pericardial cells regenerate in the second stage, and vascular endothelial cells regenerate in the final stage.

In order to conveniently observe whether a tested sample has the effect of promoting myocardial regeneration and repair, the transgenic zebra fish (Tg-fli1-eGFP) is used for carrying out an experiment, and the cardiac freezing trauma operation is carried out after the transgenic zebra fish is anesthetized. Because the zebra fish with the transferred gene can express green fluorescent protein in vascular endothelial cells, damaged cells cannot express fluorescence continuously after the frozen injury of the heart, and the size of the injured area of the heart is evaluated.

Induction of adult zebrafish ventricular frostbite

A probe having a length of about 6 mm and a width of about 0.8 mm at one end was used to cryotraumatize the ventricles of zebrafish. Prior to cryotrauma, adult zebrafish, which have been anesthetized, must be placed in the gap of the sponge with their ventral side facing up. Skin and muscle were incised between two pectoral fins of zebrafish, scissors were inserted into the incision, and the skin was incised, under the skin, fine silver subcutaneous tissue was gently opened with forceps to enter the heart, the incision was incised with forceps to expose ventricles of zebrafish, and then a cryoprobe was gently inserted into the thoracic cavity to touch the heart for cryotrauma.

When evaluating whether the traditional Chinese medicine composition can promote myocardial regeneration and repair, the heart of the zebra fish needs to be extracted to evaluate the damage condition. The procedure of heart extraction requires a deep and long incision through the gill arch of the chest to access the pericardial space, where the structures of both the aortic bulb and the ventricle are visible, followed by grasping the aortic bulb with the arterial clamp and pulling out from the body space, and then cutting the entire heart from the body to complete the heart extraction.

Any experimental process related to the induction of the zebra fish ventricular frostbite and the heart extraction of the zebra fish is carried out like the experimental process.

Evaluation of efficacy of regeneration of cardiac muscle of zebrafish

After the transgenic zebra fish is anesthetized and subjected to heart freezing injury surgery, the fish object is fed for 7 days, 12.5 micrograms (12.5 micrograms/g) of the object is given to each gram of zebra fish every day, the fish object is sacrificed and the heart is picked up on the 8 th day, the heart injury area is observed and calculated, and whether the test object has the effect of promoting the regeneration and repair of the cardiac muscle of the zebra fish is evaluated.

Because the transgenic zebra fish can express green fluorescent protein in vascular endothelial cells, the damaged or regenerated and repaired condition of the myocardial cells can be evaluated according to the size of the area expressed by green fluorescence, the area expressed by green fluorescence does not represent the damaged area of the myocardial cells, and the proportion of the area to the whole heart is calculated, so that the percentage of the wounded area can be obtained.

Here, the calculation of the injured area of the heart was performed 7 days after feeding zebrafish that had received the heart injury with the test article containing only water as a blank group, and the injured area ratio was 25.52%. After establishing the proportion of the wound area of the blank group, the effect of the traditional Chinese medicine compositions of examples 1 to 11 on myocardial regeneration and repair was then evaluated. Groups of examples 1 to 11 were prepared with water as a solvent, test subjects containing the herbal compositions of examples 1 to 11 at a concentration of 2.5 mg/ml were prepared, and wound areas of each group were calculated after feeding cardiac-traumatized zebra fish for 7 days at the aforementioned dose of 12.5 μ g/g per day, and the results of the blank group are shown in fig. 1 and table 2.

Table 2: examples 1 to 11 and blank groups Experimental results on Zebra fish myocardial trauma repair

As shown in fig. 1 and the experimental data in table 2, after 7 days of feeding the test subjects containing the herbal compositions of examples 1 to 11, the proportion of the cardiac trauma area of the zebra fish was significantly reduced compared to the blank group, confirming that the herbal compositions of examples 1 to 11 indeed have the effect of promoting the regeneration and repair of the myocardium of the zebra fish.

Further referring to the experimental results of examples 1 to 3, the components of the Chinese medicinal composition of examples 1 to 3 were codonopsis pilosula and salviae miltiorrhizae, codonopsis pilosula and American ginseng, and salviae miltiorrhizae and American ginseng, respectively. As shown in fig. 1 and the experimental data in table 2, compared to the wound area of the group in example 3 of 16.40%, the wound areas of the groups in examples 1 and 2 of only 8.74% and 9.78%, respectively, have better effects of promoting the regeneration and repair of the myocardium of zebra fish, and thus it can be seen that the traditional Chinese medicine composition containing codonopsis pilosula can further promote the regeneration and repair of the myocardium when the traditional Chinese medicine composition contains the same weight percentage of the combination of codonopsis pilosula, salviae miltiorrhizae and radix Panacis Quinquefolii.

Referring to the experimental results of examples 4 to 10, the components of the Chinese medicinal composition of examples 4 to 10 include Codonopsis pilosula, Salvia miltiorrhiza and Panax quinquefolium at different ratios. As shown in fig. 1 and the experimental data in table 2, examples 5, 6 and 9 clearly have better effects than examples 4, 7, 8 and 10, which indicates that the ratio of codonopsis pilosula, salviae miltiorrhizae and American ginseng in the Chinese medicinal composition actually affects the effect of the composition on myocardial regeneration and repair. Further elaborating the proportions of the codonopsis pilosula, the salvia miltiorrhiza and the American ginseng contained in examples 5, 6 and 9, it can be seen that when the traditional Chinese medicine composition comprises the combination of the codonopsis pilosula, the salvia miltiorrhiza and the American ginseng, the proportion of the codonopsis pilosula is 20 to 30 weight percent, the proportion of the salvia miltiorrhiza is 20 to 60 weight percent and the proportion of the American ginseng is 10 to 55 weight percent, the excellent myocardial regeneration and repair effects are shown. Wherein, when the Chinese medicinal composition is a combination of 25 weight percent of codonopsis pilosula, 25 weight percent of salvia miltiorrhiza and 50 weight percent of American ginseng (namely, example 6), the composition has the optimal myocardial regeneration and repair effects, and only 2.92 percent of wound area remains.

In addition, referring to the experimental results of examples 7, 8 and 11, the Chinese medicinal compositions of examples 7, 8 and 11 contain a higher proportion of Codonopsis pilosula, all of which are higher than 55% by weight. As shown in fig. 1 and the experimental data in table 2, compared to the wound areas of the groups of examples 7 and 8 (14.92% and 12.18%, respectively), the wound area of the group of example 11 is only 8.00%, which shows that the effect of the traditional Chinese medicine composition on myocardial regeneration and repair can be further improved when the traditional Chinese medicine composition contains specific contents of salvia miltiorrhiza and American ginseng under the condition of high codonopsis pilosula content in the traditional Chinese medicine composition.

In summary, the present invention provides a Chinese medicinal composition and a preparation method thereof, which can be used for promoting repair and regeneration of damaged cardiac muscle cells by including at least two of codonopsis pilosula, salviae miltiorrhizae and panax quinquefolius in different proportions. Accordingly, the present invention provides a non-chemosynthetic drug, non-invasive and potentially highly developed therapeutic strategy for the healing of myocardial infarction.

Claims

1. A traditional Chinese medicine composition for promoting myocardial regeneration, which comprises at least two selected from the group consisting of the following traditional Chinese medicines: radix Codonopsis, Saviae Miltiorrhizae radix and radix Panacis Quinquefolii;

when the traditional Chinese medicine composition comprises codonopsis pilosula and salvia miltiorrhiza, wherein the dosage of the codonopsis pilosula is 40-60 weight percent and the dosage of the salvia miltiorrhiza is 40-60 weight percent based on the total weight of the traditional Chinese medicine composition;

when the traditional Chinese medicine composition comprises the salvia miltiorrhiza and the American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the salvia miltiorrhiza is 40-60 weight percent, and the American ginseng is 40-60 weight percent;

when the traditional Chinese medicine composition comprises codonopsis pilosula and American ginseng, wherein the dosage of the codonopsis pilosula is 40-60 weight percent and the dosage of the American ginseng is 40-60 weight percent based on the total weight of the traditional Chinese medicine composition;

when the traditional Chinese medicine composition comprises radix codonopsis, salvia miltiorrhiza and American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the dosage of the radix codonopsis is 20-65 weight percent, the dosage of the salvia miltiorrhiza is 10-60 weight percent, and the dosage of the American ginseng is 10-55 weight percent.

2. The Chinese medicinal composition of claim 1, comprising radix Codonopsis and at least one of Salvia miltiorrhiza and Panax quinquefolium;

when the traditional Chinese medicine composition comprises codonopsis pilosula and salvia miltiorrhiza, wherein the dosage of the codonopsis pilosula is 45-55 weight percent and the dosage of the salvia miltiorrhiza is 45-55 weight percent based on the total weight of the traditional Chinese medicine composition;

when the traditional Chinese medicine composition comprises codonopsis pilosula and American ginseng, wherein the dosage of the codonopsis pilosula is 45-55 weight percent and the dosage of the American ginseng is 45-55 weight percent based on the total weight of the traditional Chinese medicine composition;

when the traditional Chinese medicine composition comprises radix codonopsis, salvia miltiorrhiza and American ginseng, wherein the total weight of the traditional Chinese medicine composition is taken as a reference, the dosage of the radix codonopsis is 20-30 weight percent, the dosage of the salvia miltiorrhiza is 20-60 weight percent, and the dosage of the American ginseng is 10-55 weight percent.

3. The Chinese medicinal composition according to claim 1 or 2, which comprises 20 to 30 weight percent of codonopsis pilosula, 20 to 60 weight percent of salvia miltiorrhiza and 10 to 55 weight percent of American ginseng, based on the total weight of the Chinese medicinal composition.

4. The Chinese medicinal composition of claim 3, wherein the amount of Codonopsis pilosula is 20-30 wt%, the amount of Salvia miltiorrhiza is 20-30 wt%, and the amount of Panax quinquefolium is 45-55 wt%, based on the total weight of the Chinese medicinal composition.

5. The Chinese medicinal composition of claim 1, which comprises radix codonopsis pilosulae, red sage root and American ginseng, wherein the radix codonopsis pilosulae accounts for 60 to 65 weight percent, the red sage root accounts for 10 to 60 weight percent and the American ginseng accounts for 10 to 40 weight percent based on the total weight of the Chinese medicinal composition.

6. The Chinese medicinal composition of claim 5, wherein the amount of Codonopsis pilosula is 60 to 65 wt%, the amount of Salvia miltiorrhiza is 20 to 30 wt%, and the amount of Panax quinquefolium is 10 to 20 wt%, based on the total weight of the Chinese medicinal composition.

7. A preparation method of a traditional Chinese medicine composition for promoting myocardial regeneration comprises the following steps:

step (a): extracting a Chinese medicinal composition to obtain an extractive solution; and

step (b): filtering the extract and drying to obtain the traditional Chinese medicine composition for promoting myocardial regeneration as claimed in any one of claims 1 to 6.

8. The method according to claim 7, wherein in the step (b), the extraction step is carried out by heating water at a temperature of 80 to 90 ℃ for 2 to 3 hours.

9. A Chinese medicinal preparation for promoting myocardial regeneration, which comprises the Chinese medicinal composition for promoting myocardial regeneration according to any one of claims 1 to 6 and a pharmaceutically acceptable carrier.

10. Use of the composition of any one of claims 1 to 6 for preparing a medicament for promoting myocardial regeneration.