CN113533632A - Method for measuring total chlorine content in balanced salt solution for perfusion - Google Patents
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- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000000460 chlorine Substances 0.000 title claims abstract description 37
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 239000003855 balanced salt solution Substances 0.000 title claims abstract description 36
- 229910052801 chlorine Inorganic materials 0.000 title claims abstract description 36
- 230000010412 perfusion Effects 0.000 title claims abstract description 35
- 239000000243 solution Substances 0.000 claims abstract description 60
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000004375 Dextrin Substances 0.000 claims abstract description 25
- 229920001353 Dextrin Polymers 0.000 claims abstract description 25
- 235000019425 dextrin Nutrition 0.000 claims abstract description 25
- 238000004448 titration Methods 0.000 claims abstract description 23
- VFNKZQNIXUFLBC-UHFFFAOYSA-N 2',7'-dichlorofluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Cl)=C(O)C=C1OC1=C2C=C(Cl)C(O)=C1 VFNKZQNIXUFLBC-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims description 17
- 238000005303 weighing Methods 0.000 claims description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000007865 diluting Methods 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 abstract description 6
- 239000006210 lotion Substances 0.000 abstract description 2
- 238000003908 quality control method Methods 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 22
- 239000000463 material Substances 0.000 description 11
- 239000011780 sodium chloride Substances 0.000 description 11
- 239000000047 product Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 238000011160 research Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 229910021607 Silver chloride Inorganic materials 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 229960004106 citric acid Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- -1 nano silver ions Chemical class 0.000 description 2
- 238000007430 reference method Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 description 1
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 1
- 229940052299 calcium chloride dihydrate Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000000385 dialysis solution Substances 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 229940021013 electrolyte solution Drugs 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 description 1
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- CQLFBEKRDQMJLZ-UHFFFAOYSA-M silver acetate Chemical compound [Ag+].CC([O-])=O CQLFBEKRDQMJLZ-UHFFFAOYSA-M 0.000 description 1
- 229940071536 silver acetate Drugs 0.000 description 1
- 229940071575 silver citrate Drugs 0.000 description 1
- 229940100890 silver compound Drugs 0.000 description 1
- 150000003379 silver compounds Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229940087562 sodium acetate trihydrate Drugs 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229960000999 sodium citrate dihydrate Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- QUTYHQJYVDNJJA-UHFFFAOYSA-K trisilver;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Ag+].[Ag+].[Ag+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QUTYHQJYVDNJJA-UHFFFAOYSA-K 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention belongs to the technical field of total chlorine content determination of eye lotion products in the pharmaceutical industry, and particularly relates to a method for determining total chlorine content in a balanced salt solution for perfusion, which is characterized in that 10ml of the balanced salt solution for perfusion is precisely measured, 80ml of water and 0.1ml of dilute nitric acid are added, 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution are added, and 0.1mol/L of silver nitrate titration solution is used for titration, so that the color of the solution is finally changed into light pink. The method can be successfully used for measuring the total chlorine content of the balanced salt solution for perfusion, and the method is well verified by methodology, meets the measurement requirement of the total chlorine content of the balanced salt solution for perfusion, realizes the accurate quality control of the balanced salt solution for perfusion, and provides a basis for the quality and safety of products.
Description
Technical Field
The invention belongs to the technical field of total chlorine content determination of eye lotion products in the pharmaceutical industry, and particularly relates to a method for determining total chlorine content in a balanced salt solution for perfusion.
Background
The balanced salt solution for perfusion is a colorless clear liquid prepared from 0.64% of sodium chloride, 0.075% of potassium chloride, 0.048% of calcium chloride dihydrate, 0.03% of magnesium chloride hexahydrate, 0.39% of sodium acetate trihydrate and 0.17% of sodium citrate dihydrate. The clinical application is mainly used for intraocular or extraocular perfusion liquid and endoscopic washing liquid in ophthalmic surgery and washing liquid of various bone joint cavities, and can also be used for allowing various surgical washing, lavage, cleaning and the like using electrolyte solution, such as washing, rinsing or soaking of various body cavities, tissues, wounds, drainage tubes and the like which need to be sterilized, and can also be used as a carrier of pharmaceutical preparations.
The balanced salt solution for perfusion is registered and managed according to a medicine management method, the effective control of the product content is vital, the use effect of any one component is influenced by too high or too low amount of any one component, and even electrolyte disorder is caused to influence the illness safety of patients. The quality standard of the product is not recorded in Chinese pharmacopoeia, European pharmacopoeia, American pharmacopoeia and Japanese pharmacopoeia, so that a method for measuring the total chlorine content in a balanced salt solution for perfusion needs to be researched urgently.
Disclosure of Invention
The invention aims to provide a method for measuring the total chlorine content in a balanced salt solution for perfusion, which solves the problems that auxiliary materials interfere and a judgment end point cannot be found in the existing method for recording corresponding measurement quality standards in the prior art.
The realization process of the invention is as follows:
a method for measuring the total chlorine content in a balanced salt solution for perfusion includes precisely measuring 10ml of the balanced salt solution for perfusion, adding 80ml of water and 0.1ml of dilute nitric acid, adding 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and titrating with 0.1mol/L silver nitrate titrating solution to change the color of the solution into light pink finally.
Further, the preparation process of the dichloro fluorescein indicating solution comprises the following steps: weighing 0.1g of dichlorofluorescein, dissolving in 60ml of ethanol, adding 2.5ml of 0.1mol/L sodium hydroxide solution, diluting with water to 100ml, and shaking up to obtain the fluorescent powder.
Further, the preparation process of the dilute nitric acid comprises the steps of measuring 105ml of concentrated nitric acid, and diluting the concentrated nitric acid to 1000ml with water to obtain the dilute nitric acid; the preparation process of the dextrin solution comprises the steps of weighing 2g of dextrin, adding 100ml of water, heating while stirring until the dextrin is boiled, and cooling.
The invention has the following positive effects:
the method can be successfully used for measuring the total chlorine content of the balanced salt solution for perfusion, and the method is well verified by methodology, meets the measurement requirement of the total chlorine content of the balanced salt solution for perfusion, realizes the accurate quality control of the balanced salt solution for perfusion, and provides a basis for the quality and safety of products.
Drawings
FIG. 1 is a linear equation of total chlorine content.
Detailed Description
The research process of the method of the invention is as follows: at first, in order to meet the requirement of measuring the total chlorine content in the balanced salt solution for perfusion, the measuring process is determined to precisely measure 10ml of the product (the balanced salt solution for perfusion), 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution are added, and 0.1mol/L of silver nitrate titration solution is used for titration. Each 1ml of 0.1mol/L silver nitrate titration solution corresponds to 3.545mg of chlorine. Wherein, the preparation process of the dichloro-fluoro-yellow indicating liquid comprises the following steps: weighing 0.1g of dichlorofluorescein, dissolving in 60ml of ethanol, adding 2.5ml of 0.1mol/L sodium hydroxide solution, diluting with water to 100ml, and shaking up to obtain the fluorescent powder. The preparation process of the dextrin solution comprises the steps of weighing 2g of dextrin, adding 100ml of water, heating while stirring until the dextrin is boiled, and cooling.
However, when the method is used for measuring the total chlorine content, the judgment end point cannot be found due to the interference of auxiliary materials, so that the method fails in an accuracy experiment and cannot measure the total chlorine content of the product. The reason is that the interference exists in the auxiliary materials, the citric acid and the silver nitrate in the auxiliary materials can generate the precipitation of the silver citrate, and researches show that the nano silver ions can be prepared by adding the citric acid into the boiling silver nitrate, and the acetate ions and the silver ions in the auxiliary materials can generate the slightly soluble silver acetate precipitation, so that when the method is used for measuring, the blank auxiliary materials are changed into orange from yellow green before titration and generate the precipitation, the color change of the sample solution is gradually changed into milk white from yellow green, no pink titration endpoint occurs, and the total chlorine content of the product cannot be measured.
Then, by researching the adding amount of the indicator, the developing pH value and the sample titration concentration, the method disclosed by the invention is determined to be obvious in endpoint judgment under the condition that 80ml of water and 0.1ml of dilute nitric acid are added on the basis of the method. The method of the invention has good verification after research, satisfies the determination of the total chlorine content in the balanced salt solution for perfusion, eliminates the interference of auxiliary materials on the determination end point, and is successfully used for determining the total chlorine content in the balanced salt solution for perfusion.
Finally, the method of the invention is determined as follows: precisely measuring 10ml of the product (balanced salt solution for perfusion), adding 80ml of water and 0.1ml of dilute nitric acid, adding 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and titrating with 0.1mol/L of silver nitrate titration solution to finally change the solution color into light pink. Each 1ml of 0.1mol/L silver nitrate titration solution corresponds to 3.545mg of chlorine. Wherein, the preparation process of the dichloro-fluoro-yellow indicating liquid comprises the following steps: weighing 0.1g of dichlorofluorescein, dissolving in 60ml of ethanol, adding 2.5ml of 0.1mol/L sodium hydroxide solution, diluting with water to 100ml, and shaking up to obtain the fluorescent powder. The preparation process of the dilute nitric acid comprises the steps of measuring 105ml of concentrated nitric acid, and diluting the concentrated nitric acid to 1000ml with water to obtain the dilute nitric acid; the preparation process of the dextrin solution comprises the steps of weighing 2g of dextrin, adding 100ml of water, heating while stirring until the dextrin is boiled, and cooling.
Calculating the formula:
in the formula: 4.5754 prescription concentration of balanced salt solution for perfusion, mg/ml
F is the F value of silver nitrate titration solution (0.1mol/L)
V is the volume of consumed silver nitrate titration solution (0.1mol/L), ml
The working principle related to the method of the invention is as follows:
dichlorofluorescein is an organic weak acid, denoted HFI. Dissociation into fluorescent yellow anions FI in solution-It is yellow green. When dichlorofluorescein is used as indicator, Cl in solution is present before the stoichiometric point-Excessive, AgCl colloidal particles are negatively charged, so FI-Is not adsorbed. After reaching the stoichiometric point, excess AgNO3Thereby leading the AgCl colloidal particles to have positive charges. At this time, the positively charged colloidal particles strongly adsorb FI-. The end point may be indicated by a color change resulting from the formation of a fluorescent yellow silver compound on the AgCl surface, causing the precipitate surface to appear pinkish. Dextrin is used as a protective colloid to prevent excessive coagulation of AgCl precipitate, and the color of the solution in the titration process is yellow green, light yellow, milky white and light pink.
The determination process of each parameter in the research process is as follows:
(1) investigation of the amount of Water added to test sample
Simultaneously taking 10ml of blank auxiliary materials and 10ml of balanced salt solution for perfusion respectively, carrying out titration according to a reference method when 0ml, 30ml, 40ml, 50ml, 60ml, 70ml, 80ml, 90ml and 100ml of water are added, and recording experimental phenomena, wherein the results are as follows:
according to the research, when the water is added to 80ml or more, no precipitate is generated in the blank auxiliary material, but the color change of the end point is not obvious at the moment, and the weak pink color can be observed only by careful attention, so the pH value (the amount of diluted nitric acid added) of the solution and the addition amount of the indicator need to be researched on the premise that the water is added to 80 ml.
(2) pH (amount of dilute nitric acid added) study of the samples
Through literature reference, the color change range of the dichlorofluorescein indicator is known to be 4-6, so that the pH of the blank auxiliary material and the pH of the sample are measured by a pH meter, the pH of the solution is adjusted to be 4-6 by the amount of the added dilute nitric acid, and the adding amount of the dilute nitric acid is determined by observing the color change condition of the titrated sample solution. The results are as follows:
in this experiment, when the amount of the dilute nitric acid added was 0.1ml or 0.12ml, the light pink color at the end of the titration was easily observed, and the color of the other amounts of dilute nitric acid was lighter than that of the 0.1ml or 0.12ml dilute nitric acid, so the amount of the dilute nitric acid added was determined to be 0.1 ml.
(3) Study on the amount of indicator (dichlorofluorescein indicator)
When the total chlorine content of the peritoneal dialysis solution product of the company is measured by using a reference method, the pink color of the end point is particularly obvious, but only a light pink color can be observed by using the method in the current research stage, and the current addition amount of the indicator is 8 drops, namely 0.4ml, considering whether the addition amount of the indicator can be increased to make the color of the end point more obvious. Now consider the addition of 0.6ml, 0.7ml, 0.8ml of indicator and observe the color change at the end of the titration. The results are as follows:
| amount of indicator added (ml) | After titration (addition of 13ml of titrant) |
| 0.4 | Is light pink |
| 0.6 | Is light pink |
| 0.7 | Is light pink and slightly yellow |
| 0.8 | Light pink color is not easy to observe and is a little yellow |
It was found that the more the indicator was added, the more yellow the color after titration and the less easily the end point color was judged, so that the amount of the indicator added was still set to 0.4ml, i.e., 8 drops.
In conclusion, the sequential research is carried out on the adding amount of water, pH (dilute nitric acid) and the indicator in the test sample, and the finding that when 80ml of water is added in the basic scheme, no precipitate is generated in the measurement of the blank auxiliary material, the measurement result cannot be interfered, and 0.1ml of dilute nitric acid is added to adjust the pH of the solution to about 5.0, so that the end point judgment of the indicator is obvious, and the color of the solution is changed from yellow green to light yellow to milky white to light pink. The preparation process of the dilute nitric acid comprises the steps of measuring 105ml of concentrated nitric acid and diluting the concentrated nitric acid to 1000ml with water.
The methodology of the present invention was verified as follows:
(1) specificity
Preparing a reference substance solution: precisely weighing 754.2mg of sodium chloride purity standard substance dried at 110 ℃ to constant weight, placing in a 100ml volumetric flask, diluting with water to scale, and shaking up.
The total chlorine measurements were performed on negative controls (blank water and blank adjuvant), positive controls (control solution) and test article (balanced salt solution for perfusion) as follows:
total chlorine content specificity results record
(2) Precision degree
Taking the same batch of balanced salt solution for perfusion, preparing 6 parts of the balanced salt solution for the experimenter A and the experimenter B simultaneously in parallel, measuring the total chlorine content according to the method, and calculating the total chlorine content, wherein the RSD of the repeatability and the intermediate precision is less than or equal to 2.0 percent.
Total chlorine content intermediate precision results record
(3) Accuracy of
The test of the recovery rate of the product is designed to adopt a sample adding method to carry out tests according to 80%, 100% and 120% of the marked concentration of chlorine content, and titrate the test by silver nitrate titration solution (0.1034 mol/L). The preparation process of the dilute nitric acid comprises the steps of measuring 105ml of concentrated nitric acid, and diluting the concentrated nitric acid to 1000ml with water to obtain the dilute nitric acid; the preparation process of the dextrin solution comprises the steps of weighing 2g of dextrin, adding 100ml of water, heating while stirring until the dextrin is boiled, and cooling.
Preparing a sample solution: precisely measuring 10ml of balanced salt solution for perfusion, putting the balanced salt solution into a 250ml conical flask, adding 80ml of water and 0.1ml of dilute nitric acid, adding 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and uniformly mixing to obtain 2 parts in parallel.
Sample preparation with 80% accuracy: precisely weighing 61.6mg, 61.2mg and 62.6mg of sodium chloride purity standard substances dried to constant weight at 110 ℃, respectively placing the sodium chloride purity standard substances into 250ml conical flasks, precisely adding 10ml of balanced salt solution for perfusion, 80ml of water and 0.1ml of dilute nitric acid, adding 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and uniformly mixing to obtain the sodium chloride sample.
Sample preparation with 100% accuracy: respectively and precisely weighing 76.2mg, 76.6mg and 75.4mg of sodium chloride purity standard substances dried to constant weight at 110 ℃, respectively placing the sodium chloride purity standard substances into 250ml conical flasks, respectively and precisely adding 10ml of balanced salt solution for perfusion, 80ml of water and 0.1ml of dilute nitric acid, 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and uniformly mixing to obtain the sodium chloride sample.
Sample preparation with 120% accuracy: respectively and precisely weighing 92.0mg, 93.7mg and 91.4mg of sodium chloride purity standard substances dried to constant weight at 110 ℃, respectively placing the standard substances into 250ml conical flasks, respectively and precisely adding 10ml of balanced salt solution for perfusion, 80ml of water and 0.1ml of dilute nitric acid, 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution, and uniformly mixing to obtain the sodium chloride indicator solution.
The solution was titrated with silver nitrate titration solution (0.1030mol/L) and the results are shown in the following table:
total chlorine content accuracy results record
(4) Linear range
Linear control stock solutions: precisely weighing 7.54g of standard sodium chloride dried to constant weight at 110 ℃, placing in a 100ml volumetric flask, diluting to the scale with water, and shaking up.
Linear solution: precisely measuring the linear control stock solutions of 5ml, 8ml, 9ml, 10ml, 11ml and 12ml, respectively placing in a 100ml measuring flask, diluting with water to scale, shaking up to obtain linear solutions of 50%, 80%, 90%, 100%, 110% and 120%.
Taking each linear solution, and determining according to an optimized method. Linear regression analysis was performed with concentration (C) as abscissa (X-axis) and volume of consumed silver nitrate titrant (V) as ordinate (Y-axis), see fig. 1. The results are shown in the following table:
total chlorine content Linear Range results recording
(5) Durability (solution stability)
6 parts of sample solution is taken, silver nitrate titration solution (0.1034mol/L) is used for titration, 3 parts are respectively measured in 0h and 2h, the total chlorine content in 0h and 2h is calculated, RD is less than or equal to 2.0%, and the stability is inspected.
Total chlorine content durability results record
In conclusion, the specificity, precision, linearity range and durability of the method of the invention all meet the acceptable standards.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and is not intended to limit the invention to the particular forms disclosed. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (3)
1. A method for determining the total chlorine content in a balanced salt solution for perfusion, comprising: 10ml of balanced salt solution for perfusion is precisely measured, 80ml of water and 0.1ml of dilute nitric acid are added, 5ml of dextrin solution and 8 drops of dichlorofluorescein indicator solution are added, and the solution is titrated by 0.1mol/L of silver nitrate titration solution, so that the color of the solution is finally changed into light pink.
2. The method for determining the total chlorine content in a balanced salt solution for perfusion according to claim 1, wherein: the preparation process of the dichloro fluorescein indicating solution comprises the following steps: weighing 0.1g of dichlorofluorescein, dissolving in 60ml of ethanol, adding 2.5ml of 0.1mol/L sodium hydroxide solution, diluting with water to 100ml, and shaking up to obtain the fluorescent powder.
3. The method for determining the total chlorine content in a balanced salt solution for perfusion according to claim 1, wherein: the preparation process of the dilute nitric acid comprises the steps of measuring 105ml of concentrated nitric acid, and diluting the concentrated nitric acid to 1000ml with water to obtain the dilute nitric acid; the preparation process of the dextrin solution comprises the steps of weighing 2g of dextrin, adding 100ml of water, heating while stirring until the dextrin is boiled, and cooling.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010085344A (en) * | 2008-10-02 | 2010-04-15 | Miura Co Ltd | Quantitative method of chloride ion |
| CN104324049A (en) * | 2014-09-26 | 2015-02-04 | 浙江天瑞药业有限公司 | Method for preparing balanced salt flushing liquid used for joint operation |
| CN105628861A (en) * | 2016-02-03 | 2016-06-01 | 齐齐哈尔大学 | Method for quickly measuring content of vitamin C in Fructus lycii |
| CN106404995A (en) * | 2016-08-31 | 2017-02-15 | 大连博融新材料股份有限公司 | Method for measuring content of chlorine in mixed acid electrolyte through potentiometric titration |
| CN112255225A (en) * | 2020-09-25 | 2021-01-22 | 斯瑞尔环境科技股份有限公司 | Method for testing COD (chemical oxygen demand) of organic matters in iron-containing waste hydrochloric acid |
-
2021
- 2021-08-30 CN CN202111003712.5A patent/CN113533632A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010085344A (en) * | 2008-10-02 | 2010-04-15 | Miura Co Ltd | Quantitative method of chloride ion |
| CN104324049A (en) * | 2014-09-26 | 2015-02-04 | 浙江天瑞药业有限公司 | Method for preparing balanced salt flushing liquid used for joint operation |
| CN105628861A (en) * | 2016-02-03 | 2016-06-01 | 齐齐哈尔大学 | Method for quickly measuring content of vitamin C in Fructus lycii |
| CN106404995A (en) * | 2016-08-31 | 2017-02-15 | 大连博融新材料股份有限公司 | Method for measuring content of chlorine in mixed acid electrolyte through potentiometric titration |
| CN112255225A (en) * | 2020-09-25 | 2021-01-22 | 斯瑞尔环境科技股份有限公司 | Method for testing COD (chemical oxygen demand) of organic matters in iron-containing waste hydrochloric acid |
Non-Patent Citations (4)
| Title |
|---|
| 凌沛学: "《眼类药物的临床应用与研究》", 31 January 2002, 中国医药科技出版社, pages: 363 * |
| 刘玉明: "心脏灌注液中总氯离子的含量测定", 《基层医学论坛》, vol. 7, no. 9, 31 December 2003 (2003-12-31), pages 814 - 815 * |
| 张丽君: "《定量化学分析实验》", 31 July 1994, 东南大学出版社, pages: 106 - 107 * |
| 胡丽娟: "《化学分析技术》", 30 September 2018, 延边大学出版社, pages: 175 - 176 * |
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