CN113533281A - DNA methylation site analysis device and method based on triple helix nucleic acid structure - Google Patents
DNA methylation site analysis device and method based on triple helix nucleic acid structure Download PDFInfo
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- CN113533281A CN113533281A CN202110801049.7A CN202110801049A CN113533281A CN 113533281 A CN113533281 A CN 113533281A CN 202110801049 A CN202110801049 A CN 202110801049A CN 113533281 A CN113533281 A CN 113533281A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention discloses a DNA methylation site analysis device and method based on a triple-helix nucleic acid structure, and the device comprises an analyzer main body, a workbench, a damping component, a station plate, an anti-skid component, a sample box, a connecting component, a protective cover, a triple-helix nucleic acid structure photoelectrochemical biosensor, a fluorescence excitation lamp and a touch display screen, wherein the workbench is arranged on one side of the top of the analyzer main body, the center of the top of the workbench is fixedly connected with the bottom of the damping component, and the damping component consists of a damping plate, a single-head stud, a rubber base plate, a limit nut and a buffer spring; according to the invention, the anti-skid structure is additionally arranged, so that the problem of sample dumping and leakage in the moving process of the device is avoided, resources are saved, resampling is not needed, the analysis cost of methylation sites is reduced, and the connecting structure is additionally arranged, so that personnel can conveniently disassemble and assemble the protection part, the maintenance and replacement are rapid, the labor intensity of the personnel is reduced, and the maintenance cost of the device is saved.
Description
Technical Field
The invention relates to the technical field of analytical devices, in particular to a DNA methylation site analytical device and a DNA methylation site analytical method based on a triple helix nucleic acid structure.
Background
DNA methylation generally refers to a process of selectively adding methyl to cytosine of genomic CpG dinucleotide under the action of DNA methyltransferase to form 5mC, and can cause changes in chromatin structure, DNA conformation, DNA stability and interaction mode of DNA and protein without changing DNA sequence, thereby controlling gene expression and changing genetic expression. DNA methylation is an important epigenetic marker and plays a significant role in regulating gene expression, maintaining chromatin structure, gene imprinting, X chromosome inactivation, embryonic development and other biological processes.
At present, in the industry, devices such as a DNA methylation analyzer and the like are mainly used, and a photoelectrochemical biosensor with a double-helix or triple-helix nucleic acid structure is utilized to convert optical signals changed in the DNA methylation process into electric signals, so that methylation sites on DNA are analyzed, and the change of methylation sequence sites on one or more CpG sites is explored.
However, the existing DNA methylation site analysis device still has certain disadvantages, and the specific problems are as follows:
(1) the existing device does not perform anti-skid fixation on the sample, the sample is easy to topple and leak in the moving process, so that not only is the resource waste caused, but also the re-sampling is needed, and the analysis cost of the methylation site is increased;
(2) the protective parts of the existing device are inconvenient to disassemble after being installed, difficult to maintain and replace, high in labor intensity of personnel, time-consuming and labor-consuming, and the maintenance cost of the device is increased;
(3) the existing device has no damping function, large mechanical vibration amplitude, easy environmental interference and poor working reliability, and influences the analysis precision of methylation sites.
Disclosure of Invention
The invention aims to provide a DNA methylation site analysis device and method based on a triple helix nucleic acid structure, so as to solve the problems of easy sample leakage, high maintenance cost and large vibration amplitude in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a DNA methylation site analysis device based on a triple-helix nucleic acid structure comprises an analyzer main body, a workbench, a damping component, a station plate, an anti-skidding component, a sample box, a connecting component, a protective cover, a photoelectric chemical biosensor with a triple-helix nucleic acid structure, a fluorescence excitation lamp and a touch display screen, wherein the workbench is arranged on one side of the top of the analyzer main body, the center of the top of the workbench is fixedly connected with the bottom of the damping component, the damping component comprises a damping plate, a single-head stud, a rubber base plate, a limit nut and a buffer spring, the bottom of the damping plate is fixedly connected with the center of the top of the workbench, four corners and four sides of the top of the damping plate are fixedly connected with one end of the single-head stud, the middle section of the single-head stud is movably sleeved with the rubber base plate and the four corners and the four sides of the bottom of the station plate respectively, the limit nut is fixedly sleeved with the other end of the single-head stud, and the bottom of the limit nut is contacted with one end of the buffer spring, buffer spring activity cup joints the middle section at single-end double-screw bolt, and buffer spring's the other end contacts with the four corners and the four sides at station board top respectively, and the bottom of station board and the top fixed connection of rubber backing plate, the bottom of rubber backing plate contacts with the top of shock attenuation board.
Preferably, the center of the top of the station plate is fixedly connected with the bottom of the sample box through an anti-slip component, the anti-slip component consists of an anti-slip frame, a limiting groove, a limiting block, a mortise groove, a tenon block, a U-shaped clamping frame, a positioning hole, a positioning block, a limiting hole, a limiting column, a pressing screw hole, a pressing screw and a pressing frame, the outer edge of the bottom of the anti-slip frame is fixedly connected with the inner edge of the top of the station plate, the sample box is embedded in the center of the top of the anti-slip frame through the limiting groove and the limiting block, the U-shaped clamping frames are symmetrically installed on two sides of the top of the anti-slip frame through the mortise groove and the tenon block, the outer edges of the top of the anti-slip frame and the U-shaped clamping frame are fixedly connected with the outer edge of the bottom of the pressing frame through the positioning hole, the limiting column, the pressing screw hole and the pressing screw, the outer edge of the top of the anti-slip frame, the U-shaped clamping frame and the pressing sample box are all sleeved on the outer wall of the sample box, the center of the bottom of the inner wall of the anti-slip box is fixedly embedded with a three-slip frame, a fluorescence excitation lamp is arranged right above the photoelectrochemical biosensor with the triple-helix nucleic acid structure and fixedly connected with the front top end of the analyzer main body through a support, and a touch display screen is arranged at one corner of the front top end of the analyzer main body.
Preferably, the both sides at analysis appearance main part top are passed through coupling assembling and the fixed articulated of two angles of the positive bottom of protection casing, coupling assembling is by the connecting block, the pinhole, the square pin, the locking screw, the minor axis, shaft hole and connection otic placode are constituteed, the bottom of connecting block and the both sides fixed connection at analysis appearance main part top, the pinhole has been seted up at the center of connecting block side, the inside of pinhole is inlayed through locking screw and locking screw is fixed and is equipped with the square pin, the center of square pin one end and the one end fixed connection of minor axis, the other end of minor axis is cup jointed through the one end activity of shaft hole with the connection otic placode, the other end of connection otic placode respectively with the two angle fixed connection of the positive bottom of protection casing.
Preferably, the limiting grooves are respectively formed in four corners of the inner edge of the top of the anti-skidding frame, limiting blocks are embedded in the limiting grooves and are respectively arranged at two corners of the bottom end of the front face and the bottom end of the back face of the sample box.
Preferably, the mortises are respectively arranged at two corners of the top of the U-shaped clamping frame, the tenon blocks are embedded in the mortises, the bottoms of the tenon blocks are fixedly connected with two sides of the top of the anti-skidding frame, the centers of the tops of the tenon blocks are fixedly connected with the centers of the bottoms of the positioning blocks, the positioning blocks are embedded in the positioning holes, and the positioning holes are respectively arranged at two sides of the bottom of the clamping frame.
Preferably, the limiting holes are respectively and uniformly formed in the other two sides of the top of the anti-slip frame and one side of the top of the U-shaped clamping frame, and one end of the limiting column embedded in the limiting hole is respectively connected with the other two sides of the bottom of the pressing frame.
Preferably, the compressing screw holes are respectively formed in the four corners of the outer edge of the top of the anti-skid frame, the other two corners of the top of the U-shaped clamping frame and the four corners of the outer edge of the top of the compressing frame, and compressing screws are embedded in the compressing screw holes.
Preferably, the locking screw holes are respectively formed in the centers of the front surfaces of the connecting block and the square pin, and locking screws are embedded in the locking screw holes.
A DNA methylation site analysis method based on a triple helix nucleic acid structure comprises the following steps:
step one, fixing; step two, damping; step three, irradiating; step four, analyzing;
in the first step, the limiting block is embedded into the limiting groove to enable the sample box to be embedded onto the anti-skid frame, the tenon block is embedded into the tenon groove to enable the U-shaped clamping frame to be installed on the anti-skid frame, the sample box is clamped, the positioning block is embedded into the positioning hole, the limiting column is embedded into the limiting hole, the compression screw is embedded into the compression screw hole, the compression frame is installed on the U-shaped clamping frame, and the sample box is fixed on the station board;
in the second step, in the moving process, the analyzer body transmits mechanical vibration to the damping plate through the workbench, so that the rubber base plate is contracted and deformed, the mechanical vibration with reduced amplitude is transmitted to the station plate, the buffer spring is contracted and shortened under the limitation of the limit nut, the mechanical vibration with reduced amplitude is transmitted to the sample box again, and the environmental interference is eliminated;
in the third step, the three-helix nucleic acid structure photoelectrochemical biosensor is connected to the analyzer main body through a cable, a sample to be analyzed is uniformly coated on the three-helix nucleic acid structure photoelectrochemical biosensor, then a primer is dripped on the sample, a fluorescence excitation lamp is started through a touch display screen, and the sample to be analyzed is irradiated;
in the fourth step, the sample and the primer are subjected to chemiluminescence reaction, then the sample emits fluorescence under the irradiation of a fluorescence excitation lamp, an optical signal with release point position and release intensity information is detected by the three-helix nucleic acid structure photoelectrochemical biosensor, the optical signal is converted into an electrical signal and is transmitted to the analyzer main body through a cable, and an analysis result is displayed on the touch display screen after calculation.
Compared with the prior art, the invention has the beneficial effects that: according to the DNA methylation site analysis device and method based on the triple-helix nucleic acid structure, the sample is not easy to leak, the maintenance cost is low, and the vibration amplitude is small;
(1) by additionally arranging the anti-skid structure, the problem of sample dumping and leakage in the moving process of the device is avoided, resources are saved, re-sampling is not needed, and the analysis cost of methylation sites is reduced;
(2) by additionally arranging the connecting structure, the protective parts can be conveniently disassembled and assembled by personnel, the maintenance and the replacement are quick, the labor intensity of the personnel is reduced, and the maintenance cost of the device is saved;
(3) by additionally arranging the damping structure, the mechanical vibration amplitude of the device is reduced, the environmental interference in the methylation site analysis process is reduced, the working reliability of the device is enhanced, and the analysis precision of the methylation site is improved.
Drawings
FIG. 1 is a front view of the overall structure of the present invention;
FIG. 2 is a left side view of the overall construction of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is a top view of the overall structure of the present invention;
FIG. 5 is a partial exploded view of the anti-skid assembly of the present invention;
FIG. 6 is a perspective view of a cartridge of the present invention;
FIG. 7 is a partial exploded view of the connecting assembly of the present invention;
FIG. 8 is a flow chart of a method of the present invention;
in the figure: 1. an analyzer body; 2. a work table; 3. a shock absorbing assembly; 31. a damper plate; 32. a single-ended stud; 33. a rubber pad; 34. a limit nut; 35. a buffer spring; 4. a station plate; 5. an anti-skid component; 501. an anti-slip frame; 502. a limiting groove; 503. a limiting block; 504. mortises; 505. a tenon block; 506. a U-shaped clamping frame; 507. positioning holes; 508. positioning blocks; 509. a limiting hole; 510. a limiting column; 511. pressing the screw hole; 512. a compression screw; 513. a pressing frame; 6. a sample cartridge; 7. a connecting assembly; 71. connecting blocks; 72. a pin hole; 73. square pin; 74. locking the screw hole; 75. locking the screw; 76. a minor axis; 77. a shaft hole; 78. connecting the ear plates; 8. a protective cover; 9. a triple helix nucleic acid structure photoelectrochemical biosensor; 10. a fluorescence excitation lamp; 11. a display screen is touched.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, an embodiment of the present invention is shown: a DNA methylation site analysis device based on a triple-helix nucleic acid structure comprises an analyzer body 1, a workbench 2, a shock absorption assembly 3, a station plate 4, an antiskid assembly 5, a sample box 6, a connecting assembly 7, a protective cover 8, a photoelectric chemical biosensor 9 with a triple-helix nucleic acid structure, a fluorescence excitation lamp 10 and a touch display screen 11, wherein the workbench 2 is arranged on one side of the top of the analyzer body 1, the center of the top of the workbench 2 is fixedly connected with the bottom of the shock absorption assembly 3, the shock absorption assembly 3 comprises a shock absorption plate 31, a single-head stud 32, a rubber cushion plate 33, a limit nut 34 and a buffer spring 35, the bottom of the shock absorption plate 31 is fixedly connected with the center of the top of the workbench 2, four corners and four sides of the top of the shock absorption plate 31 are fixedly connected with one end of the single-head stud 32, the middle section of the single-head stud 32 is movably sleeved with the rubber cushion plate 33 and four corners and four sides of the bottom of the station plate 4 respectively, the other end of the single-end stud 32 is fixedly sleeved with a limit nut 34, the bottom of the limit nut 34 is in contact with one end of a buffer spring 35, the buffer spring 35 is movably sleeved in the middle section of the single-end stud 32, the other end of the buffer spring 35 is in contact with four corners and four sides of the top of the station plate 4 respectively, the bottom of the station plate 4 is fixedly connected with the top of a rubber cushion plate 33, and the bottom of the rubber cushion plate 33 is in contact with the top of a damping plate 31; the center of the top of the work station plate 4 is fixedly connected with the bottom of the sample box 6 through an anti-skid component 5, the anti-skid component 5 is composed of an anti-skid frame 501, a limiting groove 502, a limiting block 503, a mortise 504, a tenon 505, a U-shaped clamping frame 506, a positioning hole 507, a positioning block 508, a limiting hole 509, a limiting column 510, a pressing screw hole 511, a pressing screw 512 and a pressing frame 513, the outer edge of the bottom of the anti-skid frame 501 is fixedly connected with the inner edge of the top of the work station plate 4, the sample box 6 is embedded in the center of the top of the anti-skid frame 501 through the limiting groove 502 and the limiting block 503, the limiting groove 502 is respectively arranged at four corners of the inner edge of the top of the anti-skid frame 501, the limiting block 503 is embedded in the limiting groove 502, the limiting blocks 503 are respectively arranged at two corners of the bottom of the front and back of the sample box 6, the U-shaped clamping frame 506 is symmetrically arranged at two sides of the top of the anti-skid frame 501 through the mortise 504 and the tenon 505, the outer edges of the top of the anti-skid frame 501 and the U-skid frame 506 are respectively through the positioning holes 507, the positioning holes 507, the outer edge of the positioning hole 501 and the positioning hole 507, A positioning block 508, a limiting hole 509, a limiting column 510, a pressing screw hole 511 and a pressing screw 512 are fixedly connected with the outer edge of the bottom of the pressing frame 513, the mortise 504 is respectively arranged at two corners of the top of the U-shaped clamping frame 506, a tenon block 505 is embedded in the mortise 504, the bottom of the tenon block 505 is fixedly connected with two sides of the top of the anti-skid frame 501, the center of the top of the tenon block 505 is fixedly connected with the center of the bottom of the positioning block 508, the positioning block 508 is embedded in the positioning hole 507, the positioning holes 507 are respectively arranged at two sides of the bottom of the pressing frame 513, the limiting hole 509 is respectively and uniformly arranged at the other two sides of the top of the anti-skid frame 501 and one side of the top of the U-shaped clamping frame 506, one end of the limiting column 510 embedded in the limiting hole 509 is respectively connected with the other two sides of the bottom of the pressing frame 513, the pressing screw holes 511 are respectively arranged at four corners of the outer edge of the top of the anti-skid frame 501, the other two corners of the top of the U-shaped clamping frame 506 and four corners of the top of the pressing frame 513, a compression screw 512 is embedded in the compression screw hole 511, the anti-skid frame 501, the U-shaped clamping frame 506 and the compression frame 513 are all sleeved on the outer wall of the sample box 6, the three-spiral nucleic acid structure photoelectrochemical biosensor 9 is fixedly embedded in the center of the bottom of the inner wall of the sample box 6, a fluorescence excitation lamp 10 is arranged right above the three-spiral nucleic acid structure photoelectrochemical biosensor 9, the fluorescence excitation lamp 10 is fixedly connected with the top end of the front face of the analyzer main body 1 through a support, a touch display screen 11 is arranged at one corner of the top end of the front face of the analyzer main body 1, and by additionally arranging the anti-skid structure, the problem of sample dumping and leakage in the moving process of the device is avoided, resources are saved, re-sampling is not needed, and the analysis cost of a methylation site is reduced; the two sides of the top of the analyzer body 1 are fixedly hinged with two corners of the bottom of the front surface of the protective cover 8 through a connecting assembly 7, the connecting assembly 7 comprises a connecting block 71, a pin hole 72, a square pin 73, a locking screw hole 74, a locking screw 75, a short shaft 76, a shaft hole 77 and a connecting lug plate 78, the bottom of the connecting block 71 is fixedly connected with the two sides of the top of the analyzer body 1, the center of the side surface of the connecting block 71 is provided with the pin hole 72, the inside of the pin hole 72 is fixedly embedded with the square pin 73 through the locking screw hole 74 and the locking screw 75, the locking screw hole 74 is respectively arranged at the centers of the front surfaces of the connecting block 71 and the square pin 73, the locking screw 75 is embedded inside the locking screw hole 74, the center of one end of the square pin 73 is fixedly connected with one end of the short shaft 76, the other end of the short shaft 76 is movably sleeved with one end of the connecting lug plate 78 through the shaft hole 77, and the other end of the connecting lug plate 78 is respectively fixedly connected with the two corners of the bottom of the front surface of the protective cover 8, through adding connection structure to make things convenient for personnel to carry out the dismouting to the protective member, the maintenance is changed swiftly, has reduced personnel intensity of labour, has practiced thrift the maintenance cost of device.
Referring to fig. 8, an embodiment of the present invention: a DNA methylation site analysis method based on a triple helix nucleic acid structure comprises the following steps: step one, fixing; step two, damping; step three, irradiating; step four, analyzing;
in the first step, the sample box 6 is embedded on the anti-skid frame 501 by embedding the limiting block 503 into the limiting groove 502, the U-shaped clamping frame 506 is installed on the anti-skid frame 501 by embedding the tenon 505 into the tenon groove 504, the sample box 6 is clamped, the positioning block 508 is embedded into the positioning hole 507, the limiting column 510 is embedded into the limiting hole 509, the compression screw 512 is embedded into the compression screw hole 511, the compression frame 513 is installed on the U-shaped clamping frame 506, and the sample box 6 is fixed on the station board 4;
in the second step, in the moving process, the analyzer body 1 transmits mechanical vibration to the damping plate 31 through the workbench 2, so that the rubber cushion plate 33 contracts and deforms, transmits the mechanical vibration with reduced amplitude to the station plate 4, further contracts and shortens the buffer spring 35 under the limitation of the limit nut 34, transmits the mechanical vibration with reduced amplitude again to the sample box 6, and eliminates environmental interference;
in the third step, the three-helix nucleic acid structure photoelectrochemical biosensor 9 is connected to the analyzer main body 1 through a cable, a sample to be analyzed is uniformly coated on the three-helix nucleic acid structure photoelectrochemical biosensor 9, then a primer is dripped on the sample, and a fluorescence excitation lamp 10 is turned on through a touch display screen 11 to irradiate the sample to be analyzed;
in the fourth step, the sample and the primer generate chemiluminescence reaction, then the fluorescence is emitted under the irradiation of the fluorescence excitation lamp 10, an optical signal with release point position and release intensity information is detected by the photoelectrochemical biosensor 9 with a triple-helix nucleic acid structure, the optical signal is converted into an electrical signal and is transmitted to the analyzer main body 1 through a cable, and an analysis result is displayed on the touch display screen 11 after calculation.
Based on the above, the present invention has the advantages that, when the present invention is used, the sample box 6 is first fitted to the anti-slip frame 501 by fitting the limit block 503 into the limit groove 502, the U-shaped clamping frame 506 is mounted to the anti-slip frame 501 by fitting the tenon block 505 into the tenon groove 504, the sample box 6 is clamped, the positioning block 508 is fitted into the positioning hole 507, the limit post 510 is fitted into the limit hole 509, the hold screw 512 is fitted into the hold screw hole 511, the hold frame 513 is mounted to the U-shaped clamping frame 506, the sample box 6 is further fixed to the station plate 4, the triple helix nucleic acid structure photoelectrochemical biosensor 9 is connected to the analyzer body 1 by a cable, the sample to be analyzed is uniformly coated on the triple helix nucleic acid structure photoelectrochemical biosensor 9, the primer is dripped onto the sample, the fluorescence excitation lamp 10 is turned on by touching the display panel 11, the sample to be analyzed is irradiated, then the sample and the primer have chemiluminescence reaction, then the fluorescence is emitted under the irradiation of the fluorescence excitation lamp 10, the optical signal with release point position and release intensity information is detected by the photoelectrochemical biosensor 9 with the triple helix nucleic acid structure, the optical signal is converted into an electrical signal and is transmitted to the analyzer body 1 through a cable, the analysis result is displayed on the touch display screen 11 after calculation, finally, in the moving process, the analyzer body 1 transmits mechanical vibration to the damping plate 31 through the worktable 2, so that the rubber cushion plate 33 contracts and deforms, and transmits the mechanical vibration with reduced amplitude to the station plate 4, so that the buffer spring 35 contracts and shortens under the limitation of the limit nut 34, transmits the mechanical vibration with reduced amplitude again to the sample box 6, eliminates environmental interference, and simultaneously the short shaft 76 can be extracted from the shaft hole 77 by separating the locking screw 75 from the locking screw hole 74, and then the square pin 73 is drawn out from the pin hole 72, so that the connecting lug plate 78 is taken down from the connecting block 71, the protection part is convenient to disassemble and assemble by personnel, the maintenance and the replacement are quick, the labor intensity of the personnel is reduced, and the maintenance cost of the device is saved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. The utility model provides a DNA methylation site analytical equipment based on triple helix nucleic acid structure, includes analysis appearance main part (1), workstation (2), damper unit (3), station board (4), anti-skidding subassembly (5), sample box (6), coupling assembling (7), protection casing (8), triple helix nucleic acid structure photoelectrochemistry biosensor (9), fluorescence excitation lamp (10) and touch display screen (11), its characterized in that: the analyzer comprises an analyzer body (1), a workbench (2) is arranged on one side of the top of the analyzer body, the center of the top of the workbench (2) is fixedly connected with the bottom of a damping component (3), the damping component (3) is composed of a damping plate (31), a single-end stud (32), a rubber base plate (33), a limit nut (34) and a buffer spring (35), the bottom of the damping plate (31) is fixedly connected with the center of the top of the workbench (2), four corners and four sides of the top of the damping plate (31) are fixedly connected with one end of the single-end stud (32), the middle section of the single-end stud (32) is movably sleeved with the four corners and four sides of the bottom of the rubber base plate (33) and the four sides of a station plate (4), the other end of the single-end stud (32) is fixedly sleeved with the limit nut (34), the bottom of the limit nut (34) is contacted with one end of the buffer spring (35), the buffer spring (35) is movably sleeved on the middle section of the single-end stud (32), the other end of the buffer spring (35) is respectively contacted with four corners and four sides of the top of the station plate (4), the bottom of the station plate (4) is fixedly connected with the top of the rubber cushion plate (33), and the bottom of the rubber cushion plate (33) is contacted with the top of the damping plate (31).
2. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 1, wherein: the center of the top of the work station plate (4) is fixedly connected with the bottom of the sample box (6) through an anti-slip component (5), the anti-slip component (5) is composed of an anti-slip frame (501), a limiting groove (502), a limiting block (503), a mortise groove (504), a tenon block (505), a U-shaped clamping frame (506), a positioning hole (507), a positioning block (508), a limiting hole (509), a limiting column (510), a pressing screw hole (511), a pressing screw (512) and a pressing frame (513), the outer edge of the bottom of the anti-slip frame (501) is fixedly connected with the inner edge of the top of the work station plate (4), the sample box (6) is embedded in the center of the top of the anti-slip frame (501) through the limiting groove (502) and the limiting block (503), the U-shaped clamping frame (506) is symmetrically installed on two sides of the top of the anti-slip frame (501) through the mortise groove (504) and the tenon block (505), and the outer edges of the top of the U-shaped clamping frame (506) are installed through the positioning hole (507), The device comprises a positioning block (508), a limiting hole (509), a limiting column (510), a pressing screw hole (511) and a pressing screw (512) which are fixedly connected with the outer edge of the bottom of a pressing frame (513), an anti-slip frame (501), a U-shaped clamping frame (506) and the pressing frame (513) are all sleeved on the outer wall of a sample box (6), a three-spiral nucleic acid structure photoelectrochemical biosensor (9) is fixedly embedded in the center of the bottom of the inner wall of the sample box (6), a fluorescence excitation lamp (10) is arranged right above the three-spiral nucleic acid structure photoelectrochemical biosensor (9), the fluorescence excitation lamp (10) is fixedly connected with the front top end of an analyzer main body (1) through a support, and a touch display screen (11) is arranged at one corner of the front top end of the analyzer main body (1).
3. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 1, wherein: the two sides of the top of the analyzer main body (1) are fixedly hinged with two corners of the bottom end of the front surface of the protective cover (8) through connecting components (7), each connecting component (7) comprises a connecting block (71), a pin hole (72), a square pin (73) and a locking screw hole (74), locking screw (75), minor axis (76), shaft hole (77) and connection otic placode (78) are constituteed, the bottom of connecting block (71) and the both sides fixed connection at analysis appearance main part (1) top, pinhole (72) have been seted up at the center of connecting block (71) side, the inside of pinhole (72) is fixed through locking screw hole (74) and locking screw (75) and is inlayed and be equipped with square pin (73), the center of square pin (73) one end and the one end fixed connection of minor axis (76), the other end of minor axis (76) passes through shaft hole (77) and connects the one end activity of otic placode (78) and cup joints, the other end of connecting otic placode (78) respectively with the two angle fixed connection of protection casing (8) positive bottom.
4. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 2, wherein: the limiting grooves (502) are respectively arranged at four corners of the inner edge of the top of the anti-slip frame (501), the limiting blocks (503) are embedded in the limiting grooves (502), and the limiting blocks (503) are respectively arranged at two corners of the bottom end of the front surface and the back surface of the sample box (6).
5. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 2, wherein: the structure is characterized in that the mortise (504) is respectively arranged at two corners of the top of the U-shaped clamping frame (506), a tenon block (505) is embedded in the mortise (504), the bottom of the tenon block (505) is fixedly connected with two sides of the top of the anti-skidding frame (501), the center of the top of the tenon block (505) is fixedly connected with the center of the bottom of the positioning block (508), the positioning block (508) is embedded in the positioning hole (507), and the positioning holes (507) are respectively arranged on two sides of the bottom of the pressing frame (513).
6. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 2, wherein: the limiting holes (509) are respectively and uniformly formed in the other two sides of the top of the anti-skid frame (501) and one side of the top of the U-shaped clamping frame (506), and one ends of the limiting columns (510) are embedded in the limiting holes (509) and the other two sides of the bottom of the pressing frame (513) respectively.
7. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structure according to claim 2, wherein: the pressing screw holes (511) are respectively formed in the four corners of the outer edge of the top of the anti-skid frame (501), the other two corners of the top of the U-shaped clamping frame (506) and the four corners of the outer edge of the top of the pressing frame (513), and pressing screws (512) are embedded in the pressing screw holes (511).
8. The apparatus for analyzing DNA methylation sites based on triple helix nucleic acid structures according to claim 3, wherein: the locking screw holes (74) are respectively formed in the centers of the front surfaces of the connecting block (71) and the square pin (73), and locking screws (75) are embedded in the locking screw holes (74).
9. A DNA methylation site analysis method based on a triple helix nucleic acid structure comprises the following steps: step one, fixing; step two, damping; step three, irradiating; step four, analyzing; the method is characterized in that:
in the first step, the sample box (6) is embedded on the anti-slip frame (501) by embedding the limiting block (503) into the limiting groove (502), the U-shaped clamping frame (506) is installed on the anti-slip frame (501) by embedding the tenon block (505) into the tenon groove (504), the sample box (6) is clamped, the positioning block (508) is embedded into the positioning hole (507), the limiting column (510) is embedded into the limiting hole (509), and the compression screw (512) is embedded into the compression screw hole (511), the compression frame (513) is installed on the U-shaped clamping frame (506), and the sample box (6) is further fixed on the station plate (4);
in the second step, in the moving process, the analyzer body (1) transmits mechanical vibration to the damping plate (31) through the workbench (2), so that the rubber cushion plate (33) contracts and deforms, the mechanical vibration with reduced amplitude is transmitted to the station plate (4), the buffer spring (35) contracts and shortens under the limitation of the limit nut (34), the mechanical vibration with reduced amplitude is transmitted to the sample box (6) again, and environmental interference is eliminated;
in the third step, the three-helix nucleic acid structure photoelectrochemical biosensor (9) is connected to the analyzer main body (1) through a cable, a sample to be analyzed is uniformly coated on the three-helix nucleic acid structure photoelectrochemical biosensor (9), a primer is dripped onto the sample, and a fluorescence excitation lamp (10) is turned on through a touch display screen (11) to irradiate the sample to be analyzed;
in the fourth step, the sample and the primer are subjected to chemiluminescence reaction, then the sample emits fluorescence under the irradiation of a fluorescence excitation lamp (10), an optical signal with release point position and release intensity information is detected by a three-helix nucleic acid structure photoelectrochemical biosensor (9), the optical signal is converted into an electrical signal and is transmitted to the analyzer main body (1) through a cable, and an analysis result is displayed on the touch display screen (11) after calculation.
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