CN113530281A - A kind of concrete crack bioremediation method - Google Patents
A kind of concrete crack bioremediation method Download PDFInfo
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- CN113530281A CN113530281A CN202010283895.XA CN202010283895A CN113530281A CN 113530281 A CN113530281 A CN 113530281A CN 202010283895 A CN202010283895 A CN 202010283895A CN 113530281 A CN113530281 A CN 113530281A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000000243 solution Substances 0.000 claims abstract description 88
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000004202 carbamide Substances 0.000 claims abstract description 39
- 239000011575 calcium Substances 0.000 claims abstract description 16
- 159000000007 calcium salts Chemical class 0.000 claims abstract description 15
- 239000012266 salt solution Substances 0.000 claims abstract description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 241000606860 Pasteurella Species 0.000 claims description 13
- 241000193395 Sporosarcina pasteurii Species 0.000 claims description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 6
- 239000001639 calcium acetate Substances 0.000 claims description 6
- 229960005147 calcium acetate Drugs 0.000 claims description 6
- 235000011092 calcium acetate Nutrition 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 30
- 230000001580 bacterial effect Effects 0.000 abstract description 29
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 16
- 241000894006 Bacteria Species 0.000 abstract description 9
- 230000008439 repair process Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052791 calcium Inorganic materials 0.000 abstract description 3
- 239000002244 precipitate Substances 0.000 abstract description 3
- 230000031018 biological processes and functions Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000005067 remediation Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 230000003213 activating effect Effects 0.000 description 10
- 238000012258 culturing Methods 0.000 description 10
- 239000002390 adhesive tape Substances 0.000 description 8
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 8
- 239000012634 fragment Substances 0.000 description 8
- 238000005406 washing Methods 0.000 description 8
- 241001668579 Pasteuria Species 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 241000193469 Clostridium pasteurianum Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000007790 scraping Methods 0.000 description 4
- 230000035699 permeability Effects 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000003822 epoxy resin Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229920000647 polyepoxide Polymers 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Images
Classifications
-
- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04G—SCAFFOLDING; FORMS; SHUTTERING; BUILDING IMPLEMENTS OR AIDS, OR THEIR USE; HANDLING BUILDING MATERIALS ON THE SITE; REPAIRING, BREAKING-UP OR OTHER WORK ON EXISTING BUILDINGS
- E04G23/00—Working measures on existing buildings
- E04G23/02—Repairing, e.g. filling cracks; Restoring; Altering; Enlarging
- E04G23/0203—Arrangements for filling cracks or cavities in building constructions
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- Engineering & Computer Science (AREA)
- Architecture (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Mechanical Engineering (AREA)
- Civil Engineering (AREA)
- Structural Engineering (AREA)
- Working Measures On Existing Buildindgs (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明提供了一种混凝土裂缝生物修复方法，该方法通过将巴氏芽孢杆菌溶液、尿素溶液和可溶性钙盐溶液依次注入混凝土裂缝中，多余的液体从混凝土裂缝中渗出停止操作，1小时后，重复操作，从而修复混凝土裂缝。该方法利用巴氏芽孢杆菌分解尿素，然后再钙源存在的情况下，生成碳酸钙沉淀，从而修复混凝土裂缝。本发明以细菌、尿素和可溶性钙盐为产生碳酸钙的原料，廉价易得。本发明细菌修复过程简单易操作，而且产生碳酸钙的过程为生物过程，环境友好。The invention provides a concrete crack bioremediation method. The method injects pasteurized bacillus solution, urea solution and soluble calcium salt solution into concrete cracks in sequence, and the excess liquid seeps out of concrete cracks to stop the operation, and the operation is stopped after 1 hour. , repeat the operation to repair concrete cracks. The method utilizes Bacillus pasteuri to decompose urea, and then in the presence of a calcium source, calcium carbonate precipitates are formed, thereby repairing concrete cracks. The invention uses bacteria, urea and soluble calcium salts as raw materials for producing calcium carbonate, which is cheap and easy to obtain. The bacterial restoration process of the invention is simple and easy to operate, and the process of producing calcium carbonate is a biological process, which is environmentally friendly.
Description
Technical Field
The invention belongs to the technical field of concrete crack repair, and particularly relates to a method for repairing concrete cracks by decomposing urea and inducing calcium carbonate precipitation through bacillus pasteurii.
Background
Concrete is one of the most important building materials in the world. However, the tensile strength of concrete is low, and cracks are inevitably generated. After concrete cracks appear, many harmful substances such as acid, alkali, gas and water invade the interior of the concrete portion through a single mechanism or a combined mechanism such as diffusion, capillary action, permeation, etc., resulting in corrosion of steel bars and deterioration of concrete structures. If other harmful substances reach the inside of the concrete, they may react with the components of the concrete or directly damage the structure of the concrete, thereby seriously affecting the durability of the concrete. Therefore, how to effectively repair the concrete cracks is very important.
Although the existing traditional concrete crack repairing methods such as epoxy resin injection, gravity filling, polymer impregnation and the like have good engineering effects, the used materials such as epoxy resin, carbamate and the like are easy to age, have low heat resistance and different thermal expansion coefficients with concrete, and the repaired concrete crack may be secondarily cracked, which is not acceptable for economic and safety reasons.
Aiming at the problems, the method utilizes the microorganism to induce and generate calcium carbonate precipitation so as to repair the concrete cracks, is a novel biological method, is environment-friendly and is easy to operate. Through research, the bacillus pasteurii (Sporosarcina pasteurii) can repair concrete cracks by decomposing urea and rapidly generating calcium carbonate precipitates in the presence of a calcium source. And through patent retrieval, the research on repairing concrete cracks by using the bacillus pasteurianus is not yet available.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for repairing concrete cracks by decomposing urea and inducing calcium carbonate precipitation by using bacillus pasteurianus. According to the method, the bacillus pasteurii is used for decomposing urea, and then calcium carbonate precipitates are generated under the condition that a calcium source exists, so that concrete cracks are repaired.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a biological repairing method for concrete cracks comprises the steps of sequentially injecting a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution into concrete cracks, enabling redundant liquid to seep out of the concrete cracks to stop operation, and repeating the operation after 1 hour, so that the concrete cracks are repaired.
The concentration of the soluble calcium salt solution is 1-2 mol/L.
The concentration of the urea solution is 1-2 mol/L.
The pasteuria bacillus solution is 1.2 x 108cell·mL-1。
The injection volumes of the pasteuria bacillus solution, the urea solution and the soluble calcium salt solution are the same.
The soluble calcium salt solution is Ca (NO)3)2Solution, calcium chloride solution or calcium acetate solution.
The culture temperature of the pasteurella bacillus solution is 10-40 ℃, and the culture time is 15-18 hours.
Preferably, the culture temperature of the pasteuria bacillus solution is 20 ℃.
The invention has the following beneficial effects and advantages:
(1) the invention takes bacteria, urea and soluble calcium salt as raw materials for producing calcium carbonate, and is cheap and easy to obtain.
(2) The bacterial repairing process is simple and easy to operate, and the process of producing calcium carbonate is a biological process and is environment-friendly.
(3) Ultrasonic pulse velocity detection analysis of the bacterially repaired concrete specimen revealed a decrease in propagation time of the repaired concrete specimen, indicating that the calcium carbonate sealed the cracks, thus allowing the ultrasonic waves to follow a more direct path through the sealed cracks.
(4) And (3) performing deflection analysis on the concrete sample after the bacterial remediation, and finding that the strength of the concrete sample is sequenced to the extent that the concrete sample after the bacterial remediation is larger than the concrete sample without the remediation, which shows that the strength of the concrete sample after the bacterial remediation is restored to a certain degree.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1: the invention has the overall technical route schematic diagram;
FIG. 2: the growth curve diagram of the pasteurella in the invention at different temperatures;
FIG. 3: the invention relates to a concrete sample entity diagram after repairing bacillus licheniformis;
FIG. 4: the invention relates to a permeability experimental device diagram;
FIG. 5: the transmission time schematic diagram of the concrete sample before and after the crack is repaired by the bacteria by the ultrasonic wave;
FIG. 6: load-mid-span deflection curves of the concrete sample BA after bacterial remediation and the unrepaired concrete sample W;
wherein BA refers to a concrete test piece after bacterial remediation; BB refers to the concrete specimen prior to bacterial remediation.
Detailed Description
For a better understanding of the nature of the invention, reference will now be made to the following examples taken in conjunction with the accompanying drawings.
Example 1
The invention provides a concrete crack repairing method, which comprises the steps of sequentially injecting a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution into a concrete crack, enabling redundant liquid to seep out of the concrete crack to stop operation, and repeating the operation after 1 hour, so that the concrete crack is repaired.
The concentration of the soluble calcium salt solution is 1 mol/L.
The concentration of the urea solution is 1 mol/L.
The injection volumes of the pasteuria bacillus solution, the urea solution and the soluble calcium salt solution are the same.
The soluble calcium salt solution is Ca (NO)3)2And (3) solution.
The culture temperature of the pasteurella bacillus solution is 20 ℃, and the culture time is 16 hours.
Example 2
The production process flow adopted by the invention is shown in figure 1, and the adopted technical scheme is as follows:
and (3) activating and culturing bacteria: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
determining the optimal temperature and the optimal culture time for the growth of the bacteria: culturing Pasteurella pasteurianus at 6 temperatures (0 deg.C, 5 deg.C, 10 deg.C, 20 deg.C, 30 deg.C and 40 deg.C), and plotting growth curve to obtain optimal culture temperature of 20 deg.C and optimal culture time of 15-18 hr, the result is shown in FIG. 2;
determining the optimum of the Urea solution and the calcium nitrate solutionConcentration and proportion: three sets of experiments were designed, the first set of 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2The second group was 10ml of 2mol/l urea solution, 10ml of 2mol/l Ca (NO)3)2Solution and 10ml of bacterial solution, the third group being 1.5g urea, 15ml 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution. Mixing the above three experimental solutions, reacting completely, removing supernatant after calcium carbonate is completely precipitated, drying at 100 deg.C, and measuring CaCO3Until constant weight, five replicates. The calcium carbonate quality of three experiments was compared and the results are shown in table 1;
TABLE 1 three groups of experimentally weighed CaCO3Quality of
Urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
The concrete sample of the crack after the bacterial remediation is shown in fig. 3, a constant water head permeability test is carried out, and through 24-hour observation, the crack of the sample after the bacterial remediation is found not to have any leakage, and a permeability experimental device is shown in fig. 4.
Ultrasonic pulse velocity detection analysis of the bacterially remediated concrete specimen, as shown in figure 5, found that the propagation time of the remediated concrete specimen was reduced, indicating that the calcium carbonate sealed the cracks, thus allowing the ultrasonic waves to follow a more direct path through the sealed cracks.
The concrete samples after bacterial remediation are subjected to deflection analysis, and the strength of the concrete samples is sequenced to be that the concrete samples after bacterial remediation are larger than the concrete samples without remediation, which shows that the strength of the concrete samples after bacterial remediation is restored to a certain degree, as shown in fig. 6.
Example 3
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the pasteuria bacillus at 20 ℃ for 15 hours;
urea solution, Ca (NO)3)2The optimal reaction ratio of the solution and the bacterial solution is as follows: the first group was 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 4
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
urea solution, Ca (NO)3)2The optimal reaction ratio of the solution and the bacterial solution is as follows: the first group was 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 5
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
the optimal reaction proportion of the urea solution, the calcium acetate solution and the bacterial solution is as follows: a first group of 10ml of 1mol/l urea solution, 10ml of 1mol/l calcium acetate solution and 10ml of bacteria solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, calcium acetate solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium acetate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 6
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
the optimal reaction ratio of the urea solution, the calcium chloride solution and the bacteria solution is as follows: a first group of 10ml of 1mol/l urea solution, 10ml of 1mol/l calcium chloride solution and 10ml of bacteria solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, calcium chloride solution and bacteria solution grouting: injecting the bacterial solution to be used into the concrete crack by using a pipette, then injecting 1mol/l urea solution with the same volume, and finally adding 1mol/l calcium chloride solution with the same volume;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Claims (8)
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| CN114349381A (en) * | 2022-01-27 | 2022-04-15 | 西安交通大学 | Method for modifying concrete recycled aggregate based on urease-induced calcium carbonate method |
| CN115626790A (en) * | 2022-11-02 | 2023-01-20 | 中铁四局集团有限公司 | Biological material for rapidly repairing concrete microcracks and preparation method thereof |
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| CN117800759A (en) * | 2024-01-05 | 2024-04-02 | 丹阳市基零佳崮新型建筑材料有限公司 | Method for curing concrete by utilizing microorganism-induced calcium carbonate deposition technology |
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Application publication date: 20211022 |