CN113530281A - Concrete crack biological repairing method - Google Patents

Concrete crack biological repairing method Download PDF

Info

Publication number
CN113530281A
CN113530281A CN202010283895.XA CN202010283895A CN113530281A CN 113530281 A CN113530281 A CN 113530281A CN 202010283895 A CN202010283895 A CN 202010283895A CN 113530281 A CN113530281 A CN 113530281A
Authority
CN
China
Prior art keywords
solution
concrete
concrete crack
urea
crack
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010283895.XA
Other languages
Chinese (zh)
Inventor
加尼·阿卜杜勒·瑞泽普
赵婷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nankai University
Original Assignee
Nankai University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nankai University filed Critical Nankai University
Priority to CN202010283895.XA priority Critical patent/CN113530281A/en
Publication of CN113530281A publication Critical patent/CN113530281A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • EFIXED CONSTRUCTIONS
    • E04BUILDING
    • E04GSCAFFOLDING; FORMS; SHUTTERING; BUILDING IMPLEMENTS OR AIDS, OR THEIR USE; HANDLING BUILDING MATERIALS ON THE SITE; REPAIRING, BREAKING-UP OR OTHER WORK ON EXISTING BUILDINGS
    • E04G23/00Working measures on existing buildings
    • E04G23/02Repairing, e.g. filling cracks; Restoring; Altering; Enlarging
    • E04G23/0203Arrangements for filling cracks or cavities in building constructions

Landscapes

  • Engineering & Computer Science (AREA)
  • Architecture (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Mechanical Engineering (AREA)
  • Civil Engineering (AREA)
  • Structural Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Working Measures On Existing Buildindgs (AREA)

Abstract

The invention provides a concrete crack bioremediation method, which comprises the steps of sequentially injecting a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution into a concrete crack, enabling redundant liquid to seep out of the concrete crack to stop operation, and repeating the operation after 1 hour, thereby repairing the concrete crack. According to the method, the bacillus pasteurii is used for decomposing urea, and then calcium carbonate precipitates are generated under the condition that a calcium source exists, so that concrete cracks are repaired. The invention takes bacteria, urea and soluble calcium salt as raw materials for producing calcium carbonate, and is cheap and easy to obtain. The bacterial remediation process is simple and easy to operate, and the process of producing calcium carbonate is a biological process and is environment-friendly.

Description

Concrete crack biological repairing method
Technical Field
The invention belongs to the technical field of concrete crack repair, and particularly relates to a method for repairing concrete cracks by decomposing urea and inducing calcium carbonate precipitation through bacillus pasteurii.
Background
Concrete is one of the most important building materials in the world. However, the tensile strength of concrete is low, and cracks are inevitably generated. After concrete cracks appear, many harmful substances such as acid, alkali, gas and water invade the interior of the concrete portion through a single mechanism or a combined mechanism such as diffusion, capillary action, permeation, etc., resulting in corrosion of steel bars and deterioration of concrete structures. If other harmful substances reach the inside of the concrete, they may react with the components of the concrete or directly damage the structure of the concrete, thereby seriously affecting the durability of the concrete. Therefore, how to effectively repair the concrete cracks is very important.
Although the existing traditional concrete crack repairing methods such as epoxy resin injection, gravity filling, polymer impregnation and the like have good engineering effects, the used materials such as epoxy resin, carbamate and the like are easy to age, have low heat resistance and different thermal expansion coefficients with concrete, and the repaired concrete crack may be secondarily cracked, which is not acceptable for economic and safety reasons.
Aiming at the problems, the method utilizes the microorganism to induce and generate calcium carbonate precipitation so as to repair the concrete cracks, is a novel biological method, is environment-friendly and is easy to operate. Through research, the bacillus pasteurii (Sporosarcina pasteurii) can repair concrete cracks by decomposing urea and rapidly generating calcium carbonate precipitates in the presence of a calcium source. And through patent retrieval, the research on repairing concrete cracks by using the bacillus pasteurianus is not yet available.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for repairing concrete cracks by decomposing urea and inducing calcium carbonate precipitation by using bacillus pasteurianus. According to the method, the bacillus pasteurii is used for decomposing urea, and then calcium carbonate precipitates are generated under the condition that a calcium source exists, so that concrete cracks are repaired.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a biological repairing method for concrete cracks comprises the steps of sequentially injecting a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution into concrete cracks, enabling redundant liquid to seep out of the concrete cracks to stop operation, and repeating the operation after 1 hour, so that the concrete cracks are repaired.
The concentration of the soluble calcium salt solution is 1-2 mol/L.
The concentration of the urea solution is 1-2 mol/L.
The pasteuria bacillus solution is 1.2 x 108cell·mL-1
The injection volumes of the pasteuria bacillus solution, the urea solution and the soluble calcium salt solution are the same.
The soluble calcium salt solution is Ca (NO)3)2Solution, calcium chloride solution or calcium acetate solution.
The culture temperature of the pasteurella bacillus solution is 10-40 ℃, and the culture time is 15-18 hours.
Preferably, the culture temperature of the pasteuria bacillus solution is 20 ℃.
The invention has the following beneficial effects and advantages:
(1) the invention takes bacteria, urea and soluble calcium salt as raw materials for producing calcium carbonate, and is cheap and easy to obtain.
(2) The bacterial repairing process is simple and easy to operate, and the process of producing calcium carbonate is a biological process and is environment-friendly.
(3) Ultrasonic pulse velocity detection analysis of the bacterially repaired concrete specimen revealed a decrease in propagation time of the repaired concrete specimen, indicating that the calcium carbonate sealed the cracks, thus allowing the ultrasonic waves to follow a more direct path through the sealed cracks.
(4) And (3) performing deflection analysis on the concrete sample after the bacterial remediation, and finding that the strength of the concrete sample is sequenced to the extent that the concrete sample after the bacterial remediation is larger than the concrete sample without the remediation, which shows that the strength of the concrete sample after the bacterial remediation is restored to a certain degree.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1: the invention has the overall technical route schematic diagram;
FIG. 2: the growth curve diagram of the pasteurella in the invention at different temperatures;
FIG. 3: the invention relates to a concrete sample entity diagram after repairing bacillus licheniformis;
FIG. 4: the invention relates to a permeability experimental device diagram;
FIG. 5: the transmission time schematic diagram of the concrete sample before and after the crack is repaired by the bacteria by the ultrasonic wave;
FIG. 6: load-mid-span deflection curves of the concrete sample BA after bacterial remediation and the unrepaired concrete sample W;
wherein BA refers to a concrete test piece after bacterial remediation; BB refers to the concrete specimen prior to bacterial remediation.
Detailed Description
For a better understanding of the nature of the invention, reference will now be made to the following examples taken in conjunction with the accompanying drawings.
Example 1
The invention provides a concrete crack repairing method, which comprises the steps of sequentially injecting a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution into a concrete crack, enabling redundant liquid to seep out of the concrete crack to stop operation, and repeating the operation after 1 hour, so that the concrete crack is repaired.
The concentration of the soluble calcium salt solution is 1 mol/L.
The concentration of the urea solution is 1 mol/L.
The injection volumes of the pasteuria bacillus solution, the urea solution and the soluble calcium salt solution are the same.
The soluble calcium salt solution is Ca (NO)3)2And (3) solution.
The culture temperature of the pasteurella bacillus solution is 20 ℃, and the culture time is 16 hours.
Example 2
The production process flow adopted by the invention is shown in figure 1, and the adopted technical scheme is as follows:
and (3) activating and culturing bacteria: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
determining the optimal temperature and the optimal culture time for the growth of the bacteria: culturing Pasteurella pasteurianus at 6 temperatures (0 deg.C, 5 deg.C, 10 deg.C, 20 deg.C, 30 deg.C and 40 deg.C), and plotting growth curve to obtain optimal culture temperature of 20 deg.C and optimal culture time of 15-18 hr, the result is shown in FIG. 2;
determining the optimum of the Urea solution and the calcium nitrate solutionConcentration and proportion: three sets of experiments were designed, the first set of 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2The second group was 10ml of 2mol/l urea solution, 10ml of 2mol/l Ca (NO)3)2Solution and 10ml of bacterial solution, the third group being 1.5g urea, 15ml 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution. Mixing the above three experimental solutions, reacting completely, removing supernatant after calcium carbonate is completely precipitated, drying at 100 deg.C, and measuring CaCO3Until constant weight, five replicates. The calcium carbonate quality of three experiments was compared and the results are shown in table 1;
TABLE 1 three groups of experimentally weighed CaCO3Quality of
Figure BDA0002447773780000041
Urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
The concrete sample of the crack after the bacterial remediation is shown in fig. 3, a constant water head permeability test is carried out, and through 24-hour observation, the crack of the sample after the bacterial remediation is found not to have any leakage, and a permeability experimental device is shown in fig. 4.
Ultrasonic pulse velocity detection analysis of the bacterially remediated concrete specimen, as shown in figure 5, found that the propagation time of the remediated concrete specimen was reduced, indicating that the calcium carbonate sealed the cracks, thus allowing the ultrasonic waves to follow a more direct path through the sealed cracks.
The concrete samples after bacterial remediation are subjected to deflection analysis, and the strength of the concrete samples is sequenced to be that the concrete samples after bacterial remediation are larger than the concrete samples without remediation, which shows that the strength of the concrete samples after bacterial remediation is restored to a certain degree, as shown in fig. 6.
Example 3
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the pasteuria bacillus at 20 ℃ for 15 hours;
urea solution, Ca (NO)3)2The optimal reaction ratio of the solution and the bacterial solution is as follows: the first group was 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 4
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
urea solution, Ca (NO)3)2The optimal reaction ratio of the solution and the bacterial solution is as follows: the first group was 10ml of 1mol/l urea solution, 10ml of 1mol/l Ca (NO)3)2Solution and 10ml of bacterial solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, Ca (NO)3)2Solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium nitrate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 5
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
the optimal reaction proportion of the urea solution, the calcium acetate solution and the bacterial solution is as follows: a first group of 10ml of 1mol/l urea solution, 10ml of 1mol/l calcium acetate solution and 10ml of bacteria solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, calcium acetate solution and bacterial solution grouting: injecting the bacterial solution to be used into a concrete crack by using a pipette, then injecting an equal volume of 1mol/l urea solution, and finally adding an equal volume of 1mol/l calcium acetate solution;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.
Example 6
B, activating and culturing the bacillus pasteurii: activating and pouring the pasteurella freeze-dried powder purchased by the Beinanbiology into a flat plate, and carrying out expanded culture;
culturing the Pasteurella pasteuriana at 20 ℃ for 18 hours;
the optimal reaction ratio of the urea solution, the calcium chloride solution and the bacteria solution is as follows: a first group of 10ml of 1mol/l urea solution, 10ml of 1mol/l calcium chloride solution and 10ml of bacteria solution;
cleaning concrete cracks: firstly, placing a concrete sample under a faucet for washing so as to remove some fragments in cracks, then scraping some other fragments by using a feeler gauge, placing the concrete sample under the faucet for washing again, and finally drying at room temperature; and sticking a waterproof adhesive tape at the bottom of the crack. The waterproof adhesive tape has the functions of allowing excessive water to pass through, and the repairing material is kept in the crack;
urea solution, calcium chloride solution and bacteria solution grouting: injecting the bacterial solution to be used into the concrete crack by using a pipette, then injecting 1mol/l urea solution with the same volume, and finally adding 1mol/l calcium chloride solution with the same volume;
stopping the operation when excess water seeps out of the crack; after 1 hour, the above operation was repeated until the fracture was repaired completely.

Claims (8)

1. A concrete crack bioremediation method is characterized in that: according to the method, a bacillus pasteurianus solution, a urea solution and a soluble calcium salt solution are sequentially injected into a concrete crack, redundant liquid seeps out of the concrete crack to stop operation, and the operation is repeated after 1 hour, so that the concrete crack is repaired.
2. The concrete crack bioremediation method of claim 1, wherein: the concentration of the soluble calcium salt solution is 1-2 mol/L.
3. The concrete crack bioremediation method of claim 1, wherein: the concentration of the urea solution is 1-2 mol/L.
4. The concrete crack bioremediation method of claim 1, wherein: the concentration of the Pasteurella solution is 1.2 multiplied by 108cell·mL-1
5. The concrete crack bioremediation method of claim 1, wherein: the injection volumes of the pasteuria bacillus solution, the urea solution and the soluble calcium salt solution are the same.
6. The concrete crack bioremediation method of claim 1, wherein: the soluble calcium salt solution is Ca (NO)3)2Solution, calcium chloride solution or calcium acetate solution.
7. The concrete crack bioremediation method of claim 1, wherein: the culture temperature of the pasteurella bacillus solution is 10-40 ℃, and the culture time is 15-18 hours.
8. The concrete crack bioremediation method of claim 1, wherein: the culture temperature of the pasteuria bacillus solution is 20 ℃.
CN202010283895.XA 2020-04-13 2020-04-13 Concrete crack biological repairing method Pending CN113530281A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010283895.XA CN113530281A (en) 2020-04-13 2020-04-13 Concrete crack biological repairing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010283895.XA CN113530281A (en) 2020-04-13 2020-04-13 Concrete crack biological repairing method

Publications (1)

Publication Number Publication Date
CN113530281A true CN113530281A (en) 2021-10-22

Family

ID=78119836

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010283895.XA Pending CN113530281A (en) 2020-04-13 2020-04-13 Concrete crack biological repairing method

Country Status (1)

Country Link
CN (1) CN113530281A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114292127A (en) * 2021-11-30 2022-04-08 华中科技大学 High-tolerance environment-friendly microbial modifier and preparation method and application thereof
CN114349381A (en) * 2022-01-27 2022-04-15 西安交通大学 Method for modifying concrete recycled aggregate based on urease-induced calcium carbonate method
CN115626790A (en) * 2022-11-02 2023-01-20 中铁四局集团有限公司 Biological material for rapidly repairing concrete microcracks and preparation method thereof
CN117800759A (en) * 2024-01-05 2024-04-02 丹阳市基零佳崮新型建筑材料有限公司 Method for curing concrete by utilizing microorganism-induced calcium carbonate deposition technology

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101270369A (en) * 2008-05-06 2008-09-24 清华大学 Microorganism cause concrete or concrete, producing method and application thereof
CN104196131A (en) * 2014-09-11 2014-12-10 山东建筑大学 Method for plugging cast-in-situ concrete slab or base-plate crack with microbe-sedimented calcium carbonate
CN104818719A (en) * 2015-03-16 2015-08-05 山东建筑大学 Microorganism grouting method capable of improving early-stage calcium carbonate deposit volume
US20160090328A1 (en) * 2013-05-17 2016-03-31 Stichting Voor De Technische Wetenschappen Bio-based repair method for concrete

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101270369A (en) * 2008-05-06 2008-09-24 清华大学 Microorganism cause concrete or concrete, producing method and application thereof
US20160090328A1 (en) * 2013-05-17 2016-03-31 Stichting Voor De Technische Wetenschappen Bio-based repair method for concrete
CN104196131A (en) * 2014-09-11 2014-12-10 山东建筑大学 Method for plugging cast-in-situ concrete slab or base-plate crack with microbe-sedimented calcium carbonate
CN104818719A (en) * 2015-03-16 2015-08-05 山东建筑大学 Microorganism grouting method capable of improving early-stage calcium carbonate deposit volume

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
王昶力: "微生物修复混凝土裂缝的试验研究", 《硕士论文全文库》 *
贾强、姜欢、张鑫: "微生物沉积碳酸钙封堵混凝土裂缝的试验研究", 《建筑材料学报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114292127A (en) * 2021-11-30 2022-04-08 华中科技大学 High-tolerance environment-friendly microbial modifier and preparation method and application thereof
CN114349381A (en) * 2022-01-27 2022-04-15 西安交通大学 Method for modifying concrete recycled aggregate based on urease-induced calcium carbonate method
CN115626790A (en) * 2022-11-02 2023-01-20 中铁四局集团有限公司 Biological material for rapidly repairing concrete microcracks and preparation method thereof
CN115626790B (en) * 2022-11-02 2023-10-27 中铁四局集团有限公司 Biological material for rapidly repairing concrete microcracks and preparation method thereof
CN117800759A (en) * 2024-01-05 2024-04-02 丹阳市基零佳崮新型建筑材料有限公司 Method for curing concrete by utilizing microorganism-induced calcium carbonate deposition technology

Similar Documents

Publication Publication Date Title
CN113530281A (en) Concrete crack biological repairing method
Feng et al. Coupled effect of PP fiber, PVA fiber and bacteria on self-healing efficiency of early-age cracks in concrete
Liu et al. Enhancement of MICP-treated sandy soils against environmental deterioration
Xu et al. Application of ureolysis-based microbial CaCO3 precipitation in self-healing of concrete and inhibition of reinforcement corrosion
De Belie et al. Crack repair in concrete using biodeposition
Zhang et al. Effects of carrier on the performance of bacteria-based self-healing concrete
Sun et al. Glucose addition improves the bio-remediation efficiency for crack repair
Van Tittelboom et al. Use of bacteria to repair cracks in concrete
CN110563370B (en) Production process for preparing recycled aggregate from waste concrete
Qian et al. Living concrete with self-healing function on cracks attributed to inclusion of microorganisms: Theory, technology and engineering applications—A review
Luo et al. Efficiency of concrete crack-healing based on biological carbonate precipitation
CN103755195B (en) Utilize the method that urease-producing microorganism and calcium acetate prepare high intensity microorganism mortar
Zabanoot Review of autogenous and autonomous self-healing concrete technologies for marine environments
Sun et al. Adding aluminum oxide to improve the repairing effect of cracks based on bio-remediation
Hungria et al. Optimizing the self-healing efficiency of hydrogel-encapsulated bacteria in concrete
CN113913469A (en) Blast furnace crack repairing method based on microbial agent
Ihsani et al. The Utilization of Milk as a Catalyst Material in Enzyme-Mediated Calcite Precipitation (EMCP) for Crack-Healing in Concrete
CN111087192B (en) Microbial repairing agent for marine concrete crack self-repair
Gong et al. Environmental Effect of Grouting Batches on Microbial-Induced Calcite Precipitation.
CN117125915A (en) Regenerated coarse aggregate modification method based on urease mineralization deposition
Hungria et al. Self-Healing Efficiency of Cementitous Mortar Using Different Bacteria Protection Methods and Mineral Precursors
CN115626790A (en) Biological material for rapidly repairing concrete microcracks and preparation method thereof
Richardson et al. Bacterial crack sealing and surface finish application to concrete
Hu et al. Microbial repair materials based on sodium alginate modification for mortar crack repair
Rong et al. Effect of sodium alginate on the properties of microbial repair materials and its repair effect on mortar crack

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20211022

RJ01 Rejection of invention patent application after publication