CN113528339A

CN113528339A - Cell culture device and culture method thereof

Info

Publication number: CN113528339A
Application number: CN202110815653.5A
Authority: CN
Inventors: 赧兴治
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-07-19
Filing date: 2021-07-19
Publication date: 2021-10-22

Abstract

The invention relates to the technical field of cell culture, in particular to a cell culture device and a culture method thereof, which comprises a support box, a frame plate and an electric push rod, the device comprises a transfer plate, a heightening plate and a sliding plate, wherein the top end of the sliding plate is fixedly connected with a plurality of storage devices, each storage device comprises a culture dish, the bottom of the culture dish is sleeved with a support ring, the left side of the culture dish is provided with a clamping seat, the right end of the support ring is inserted into the bottom of the clamping seat, the top end of the clamping seat is provided with a rotary clamping cylinder, the output shaft end of the rotary clamping cylinder is fixedly connected with a connecting block, the left end of the connecting block is fixedly connected with a cover plate, the rear part of the top end of the support box is fixedly connected with an extension plate, the middle part of the front end of the extension plate is provided with a moving module, the output end of the moving module is fixedly connected with a cantilever plate, the middle part of the cantilever plate is fixedly connected with a discharge pipe, the top end of the discharge pipe is communicated with a guide pipe, the top end of the extension plate is fixedly connected with a mixing box, and the middle part of the bottom end of the mixing box is communicated with a connecting pipe; it is convenient for add nutrient substance, reduces the influence to the living environment inside the cell.

Description

Cell culture device and culture method thereof

Technical Field

The invention relates to the technical field of cell culture, in particular to a cell culture device and a culture method thereof.

Background

In order to reduce external interference during the cell culture process, the cells need to be placed in a relatively closed environment, and nutrients need to be added periodically during the cell culture process.

Through the search, patent No. CN208883904U discloses a cell culture apparatus. The above prior art solutions still have the following drawbacks: 1. in the using process of the device, certain nutrient substances required by experiments are difficult to be added into the culture dish, and the actual working effect is poor; 2. in addition, the cell culture device in the prior art has difficulty in adding different nutrient substances to different culture dishes; 3. the cell culture device in the prior art is difficult to mix nutrient substances which are to be added into a culture dish to a certain degree, and is poor in practicability.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a cell culture device and a cell culture method, which are convenient for adding nutrient substances and reduce the influence on the living environment inside cells.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a cell culture device comprises a support box, wherein the right end of the inner wall of the support box is fixedly connected with a frame plate, the bottom end of the frame plate is provided with an electric push rod, the output end of the electric push rod is fixedly connected with an adapter plate, the left part and the right part of the top end of the adapter plate are fixedly connected with heightening plates, the upper side of the adapter plate is provided with a sliding plate, the bottom end of the sliding plate is fixedly connected with the top ends of the heightening plates, the top end of the sliding plate is fixedly connected with a plurality of storage devices, each storage device comprises a culture dish, the bottom of the culture dish is sleeved with a support ring, the left side of the culture dish is provided with a clamping seat, the right end of the support ring is inserted into the bottom of the clamping seat, the top end of the clamping seat is provided with a rotary clamping cylinder, the output shaft end of the rotary clamping cylinder is fixedly connected with a connecting block, and the left end of the connecting block is fixedly connected with a cover plate, the middle part of the bottom end of the cover plate is provided with a abdicating groove corresponding to the upper part of the culture dish, the middle part of the top end of the support box is provided with a plurality of material passing pipes, the bottom ends of the material passing pipes are inserted into the support box from the top end of the support box, the rear part of the top end of the support box is fixedly connected with an extension plate, the middle part of the front end of the extension plate is provided with a moving module, the output end of the moving module is fixedly connected with a cantilever plate, the middle part of the cantilever plate is fixedly connected with a material discharging pipe, the top end of the material discharging pipe is communicated with a material guide pipe, the top end of the extension plate is fixedly connected with a material mixing box, the bottom end of the inner wall of the material mixing box is in a shrinkage shape from top to bottom, the left side and the right side of the material mixing box are symmetrically provided with a first electromagnetic valve and a second electromagnetic valve, the middle part of the bottom end of the material mixing box is communicated with a connecting pipe, the electromagnetic valve is arranged in the connecting pipe, the bottom of connecting pipe with the top of passage intercommunication, the top of compounding case all communicates there is the inlet pipe, each the top of inlet pipe all communicates there is the storage case.

Preferably, the sliding plates are in a step shape, the step surface of each sliding plate is fixedly connected with the bottom end of each corresponding culture dish, the bottom end of each material passing pipe is communicated with an elongated pipe, and the elongated pipe is provided with multiple length specifications.

Preferably, the middle part of each material passing pipe is fixedly connected with a limiting plate, and the bottom end of each limiting plate is connected with the top end of the supporting box through a screw.

Preferably, the inner wall of the abdicating groove is fixedly connected with a sealing gasket.

Preferably, the bottom left part and the bottom right part of the adapter plate are fixedly connected with limit shafts, the outer wall of the circumference of each limit shaft is in vertical sliding fit with the left part and the right part of the frame plate respectively, the bottom left part of the frame plate is fixedly connected with a support column, and the bottom end of the support column is fixedly connected with the bottom end of the inner wall of the support box.

Preferably, the upper part of the outer side wall of the mixing box is fixedly connected with a widening plate, and the bottom end of the storage box is fixedly connected with the top end of the widening plate.

Preferably, a photoelectric sensor is fixedly connected to the right end of the inner wall of the support box, a trigger block is installed at the right end of the sliding plate, a control panel is installed in the middle of the right end of the support box, and the photoelectric sensor and the electric push rod are electrically connected with the control panel.

A method of culturing a cell culture apparatus, comprising the steps of:

step one, starting an electric push rod to extend and retract an output shaft of the electric push rod so as to adjust the height of a culture dish, and then extending an output shaft of a rotary clamping cylinder so as to drive a cover plate on the upper part of the culture dish to open;

step two, starting the moving module, driving the discharging pipe to move left and right by the moving module, and stopping the moving module when the discharging pipe moves to a material passing pipe above the culture dish to be fed;

step three, the culture raw materials are led into each storage box, the culture raw materials are led into the mixing box through the inlet pipe to be mixed, the first electromagnetic valve and the second electromagnetic valve are opened when the culture raw materials are gathered at the first electromagnetic valve and the second electromagnetic valve, the culture raw materials are accumulated on the electromagnetic valve inside the connecting pipe, the nutrient substances are led into the material leading pipe through opening the electromagnetic valve inside the connecting pipe, the material guide pipe and the material discharging pipe, and the nutrient substances are led into the culture dish through the material leading pipe.

(III) advantageous effects

Compared with the prior art, the invention provides a cell culture device and a culture method thereof, which have the following beneficial effects:

the cell culture device and the culture method thereof drive the adapter plate, the heightening plate, the sliding block and each culture dish to ascend and descend through the electric push rod, thereby adjusting the height of the culture dish, facilitating a user to take and place the culture dish, and adjusting the height of the culture dish by driving the culture dish to ascend through the electric push rod when adding nutrient substances, reducing the waste of the nutrient substances, and after adding the nutrient substances into the mixing box, the nutrient substances added into the device can be mixed twice to a certain degree through the first electromagnetic valve, the second electromagnetic valve, the design of the vertical shrinkage of the mixing box, the connecting pipe and the electromagnetic valve at the temporal part of the connecting pipe, and the components of materials added into different culture dishes can be reasonably controlled, thereby improving the practicability of the device, after mixing the nutrient substances through the mixing box, leading the nutrient substances into the feeding pipe through the connecting pipe, the guide pipe and the discharging pipe, and drive discharging pipe shift position through removing the module, and then make the discharging pipe move to different material pipe departments that lead to, be convenient for add nutrient substance to the culture dish of difference, reduce the influence of external environment to the culture dish through supporting box and apron, reach and be convenient for add nutrient substance, reduce the effect to the influence of the inside living environment of cell.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1A;

FIG. 3 is a schematic top view of the moving die set, cantilever plate and tapping pipe of the present invention;

FIG. 4 is a schematic top view of the culture dish, the support ring, the clamping base and the rotary clamping cylinder of the present invention;

in the figure: 1. a support box; 2. a frame plate; 3. an electric push rod; 4. an adapter plate; 5. heightening plates; 6. a sliding plate; 7. a culture dish; 8. a support ring; 9. a card holder; 10. rotating the clamping cylinder; 11. connecting blocks; 12. a cover plate; 13. a material passing pipe; 14. an extension plate; 15. a moving module; 16. a cantilever plate; 17. a discharge pipe; 18. a material guide pipe; 19. a mixing box; 20. a connecting pipe; 21. a feed pipe; 22. a material storage box; 23. lengthening a pipe; 24. a limiting plate; 25. a gasket; 26. a limiting shaft; 27. a support pillar; 28. a widening plate; 29. a photosensor; 30. a trigger block; 31. a control panel; 32. a first solenoid valve; 33 second solenoid valve.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

Referring to fig. 1-4, a cell culture apparatus comprises a support box 1, a frame plate 2 is fixedly connected to the right end of the inner wall of the support box 1, an electric push rod 3 is installed at the bottom end of the frame plate 2, an adapter plate 4 is fixedly connected to the output end of the electric push rod 3, limiting shafts 26 are fixedly connected to the left portion of the bottom end and the right portion of the bottom end of the adapter plate 4, the circumferential outer walls of the limiting shafts 26 are respectively in up-and-down sliding fit with the left portion and the right portion of the frame plate 2, a supporting column 27 is fixedly connected to the left portion of the bottom end of the frame plate 2, the bottom end of the supporting column 27 is fixedly connected to the bottom end of the inner wall of the support box 1, the transverse rotation of the adapter plate 4 is reduced by the cooperation between the limiting shafts 26 and the frame plate 2, the effect of further improving the use stability is achieved, a heightening plate 5 is fixedly connected to the left portion of the top end and the right portion of the top end of the adapter plate 4, a sliding plate 6 is arranged on the upper side of the adapter plate 4, the bottom end of the sliding plate 6 is fixedly connected with the top ends of the heightening plates 5, the top end of the sliding plate 6 is fixedly connected with a plurality of storage devices, each storage device comprises a culture dish 7, a support ring 8 is sleeved at the bottom of the culture dish 7, a clamping seat 9 is arranged at the right side of the culture dish 7, the right end of the support ring 8 is inserted into the bottom of the clamping seat 9, a rotary clamping cylinder 10 is installed at the top end of the clamping seat 9, a connecting block 11 is fixedly connected with the output shaft end of the rotary clamping cylinder 10, a cover plate 12 is fixedly connected with the left end of the connecting block 11, a abdicating groove corresponding to the upper part of the culture dish 7 is arranged in the middle of the bottom end of the cover plate 12, a sealing gasket 25 is fixedly connected with the inner wall of the abdicating groove, the sealing gasket 25 is a rubber gasket, the sealing effect on the cell environment by the sealing gasket 25 is better, the influence of the external environment on the cell environment is further reduced, a plurality of material passing pipes 13 are arranged in the middle of the top end of the supporting box 1, the bottom ends of the material passing pipes 13 are inserted into the supporting box 1, the middle part of each material passing pipe 13 is fixedly connected with a limiting plate 24, and the bottom end of each limiting plate 24 is connected with the top end of the supporting box 1 through a screw; the material passing pipe 13 is convenient for a user to detach through the screw connection between the limiting plate 24 and the supporting box 1, the material passing pipe 13 is dragged through the limiting plate 24, and the deflection of the material passing pipe 13 is reduced, the extending plate 14 is fixedly connected to the rear portion of the top end of the supporting box 1, the moving module 15 is installed in the middle of the front end of the extending plate 14, the cantilever plate 16 is fixedly connected to the output end of the moving module 15, the discharging pipe 17 is fixedly connected to the middle of the cantilever plate 16, the top end of the discharging pipe 17 is communicated with the material guiding pipe 18, the material guiding pipe 18 is a rubber hose, the top end of the extending plate 14 is fixedly connected with the material mixing box 19, the bottom end of the inner wall of the material mixing box 19 is in a top-down contraction shape, the first electromagnetic valve 32 and the second electromagnetic valve 33 are symmetrically arranged on the left side and the right side of the material mixing box 19, the connecting pipe 20 is communicated with the middle portion of the bottom end of the material mixing box 19, the electromagnetic valve 20 is arranged inside the connecting pipe 20, and the top end of the material guiding pipe 18 is communicated with each other, all the intercommunication in the top of mixing box 19 has inlet pipe 21, and the top of each inlet pipe 21 all communicates has storage case 22, and mixing box 19's lateral wall upper portion fixedly connected with widening board 28, the bottom of storage case 22 all with the top fixed connection of widening board 28, widen the width on mixing box 19 upper portion through widening board 28, and then improve the space of installation storage case 22, improve the practicality.

Furthermore, the sliding plates 6 are in a ladder shape, the ladder surface of each sliding plate 6 is fixedly connected with the bottom end of each corresponding culture dish 7, the bottom end of each material passing pipe 13 is communicated with an extension pipe 23, the extension pipe 23 is provided with a plurality of length specifications, the collision between the cover plate 12 and the adjacent culture dish 7 after the cover plate 12 is rotated is reduced through the ladder-shaped sliding plates 6, nutrient substances are introduced into the culture dishes 7 through the extension pipes 23, the waste of the nutrient substances is further reduced, the different ladder heights are adapted through the extension pipes 23 with different lengths, the right end of the inner wall of the support box 1 is fixedly connected with a photoelectric sensor 29, the right end of the sliding plate 6 is provided with a trigger block 30, the trigger block 30 is an iron block, the middle part of the right end of the support box 1 is provided with a control panel 31, and the photoelectric sensor 29 and the electric push rod 3 are both electrically connected with the control panel 31, the position of the trigger block 30 is sensed by the photoelectric sensor 29, and after a signal is received by the control panel 31, the electric push rod 3 is controlled to stop, the sliding plate 6 and the culture dish 7 are automatically stopped at a specific position, the practicability is improved, the adapter plate 4, the heightening plate 5, the sliding block and each culture dish 7 are driven to ascend and descend by the electric push rod 3, the height of the culture dish 7 is adjusted by driving the culture dish 7 to ascend by the electric push rod 3 when nutrient substances are added, the waste of the nutrient substances is reduced, after the nutrient substances are added into the mixing box 19 and mixed by the mixing box 19, the nutrient substances are introduced into the material through the connecting pipe 20, the material guide pipe 18 and the material discharge pipe 17, and the material discharge pipe 17 is driven to move by the moving module 15, and then make discharging pipe 17 remove the material pipe 13 department that leads to of difference, be convenient for add nutrient substance to different culture dishes 7, reduce external environment through supporting box 1 and to the influence of culture dishes 7, reach the effect of being convenient for add nutrient substance, reduce the influence to the inside living environment of cell.

A method of culturing a cell culture apparatus, comprising the steps of:

step one, starting the electric push rod 3 to extend and retract an output shaft of the electric push rod 3 so as to adjust the height of the culture dish 7, and then extending an output shaft of the rotary clamping cylinder 10 so as to drive a cover plate 12 on the upper part of the culture dish 7 to open; the electric push rod 3 drives the culture dish 7 to move up and down, and the rotary clamping cylinder 10 drives the cover plate 12 to rotate, so that the cover plate 12 is convenient to lift, the cover plate 12 is manually lifted, and the influence of the hand of a user on the culture dish 7 is further reduced.

Step two, starting the moving module 15, driving the discharging pipe 17 to move left and right by the moving module 15, and stopping moving the moving module 15 when moving to the material passing pipe 13 above the culture dish 7 to be fed; the discharging pipe 17 is driven to move by the moving module 15, the position of the culture dish 7 is adjusted, the rereading positioning precision is high by the moving module 15, and the material passing pipe 13 is conveniently and repeatedly positioned.

Step three, introducing culture raw materials into each storage box 22, introducing the culture raw materials into a mixing box 19 through a feeding pipe 21 for mixing, opening a first electromagnetic valve 32 and a second electromagnetic valve 33 when the culture raw materials are gathered at the first electromagnetic valve 32 and the second electromagnetic valve 33, so that the culture raw materials are accumulated in the electromagnetic valves in a connecting pipe 20, introducing nutrient substances into a material passing pipe 13 by opening the electromagnetic valves in the connecting pipe 20, a material guide pipe 18 and a material discharging pipe 17, and introducing the nutrient substances into a culture dish 7 through the material passing pipe 13; through letting in nutrient substance to lead to in the material pipe 13, through the mode that leads to material pipe 13 and let in nutrient substance into culture dish 7, be convenient for adjust nutrient substance's transmission position.

To sum up, the cell culture device and the culture method thereof, when in use, firstly, a user makes the electric push rod 3 drive the adapter plate 4, the heightening plate 5, the sliding block and each culture dish 7 ascend and descend, and further adjusts the height of the culture dish 7, when the user needs to take and place the culture dish 7, the culture dish 7 descends, the electric push rod 3 drives the culture dish 7 to ascend, the height of the culture dish 7 is adjusted, the moving module 15 drives the discharging pipe 17 to move, and further the discharging pipe 17 moves to a specific material passing pipe 13, then the user adds nutrient substances into the mixing box 19, through the design of the vertical shrinkage of the mixing box 19 and the arrangement of the first electromagnetic valve 32 and the second electromagnetic valve 33, when the nutrient substances enter the mixing box 19, the nutrient substances of different types can be blocked by the first electromagnetic valve 32 and the second electromagnetic valve 33 due to the blocking of the first electromagnetic valve 32 and the second electromagnetic valve 33 and the gravity of the nutrient substances Certain impact and extrusion and then realize the mixture of certain degree, when all nutrient substances of mixing box 19 all pile up in first solenoid valve 32 and second solenoid valve 33 department, open first solenoid valve 32 and second solenoid valve 33, the solenoid valve department inside connecting pipe 20 is placed to accumulational nutrient substance in two places that make, realize the mixture of holistic certain degree, mix the back with nutrient substance, open the inside solenoid valve of connecting pipe 20, pass through connecting pipe 20 with nutrient substance, passage material 18 and discharging pipe 17 let in nutrient substance leads to in the pipe 13, let in nutrient substance inside culture dish 7 through leading to pipe 13.

The electric push rod 3, the rotary clamping cylinder 10, the photoelectric sensor 29, the moving module 15, the SM75-W-10-200 and the control panel 31 are all known devices directly purchased from the market, and are only used here, and structural and functional improvements are not performed on the devices, and details are not repeated here.

The rotary clamping cylinder 10 acts in such a way that the output shaft rotates after being lifted, and is equipped with associated pneumatic equipment, as is well known to those skilled in the art.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture apparatus, characterized by: comprises a support box (1), wherein the right end of the inner wall of the support box (1) is fixedly connected with a frame plate (2), an electric push rod (3) is installed at the bottom end of the frame plate (2), an adapter plate (4) is fixedly connected with the output end of the electric push rod (3), the left end and the right end of the top end of the adapter plate (4) are fixedly connected with an increase plate (5), a sliding plate (6) is arranged on the upper side of the adapter plate (4), the bottom end of the sliding plate (6) is fixedly connected with the top end of each increase plate (5), a plurality of storage devices are fixedly connected with the top end of the sliding plate (6), each storage device comprises a culture dish (7), a support ring (8) is sleeved at the bottom of the culture dish (7), a clamping seat (9) is arranged on the left side of the culture dish (7), and the right end of the support ring (8) is inserted into the bottom of the clamping seat (9), a rotary clamping cylinder (10) is installed at the top end of the clamping seat (9), a connecting block (11) is fixedly connected to an output shaft end of the rotary clamping cylinder (10), a cover plate (12) is fixedly connected to the left end of the connecting block (11), a receding groove corresponding to the upper portion of the culture dish (7) is formed in the middle of the bottom end of the cover plate (12), a plurality of material passing pipes (13) are arranged in the middle of the top end of the supporting box (1), the bottom ends of the material passing pipes (13) are inserted into the supporting box (1) from the top end of the supporting box (1), an extending plate (14) is fixedly connected to the rear portion of the top end of the supporting box (1), a moving module (15) is installed in the middle of the front end of the extending plate (14), a cantilever plate (16) is fixedly connected to an output end of the moving module (15), and a material discharging pipe (17) is fixedly connected to the middle of the cantilever plate (16), the top intercommunication of discharging pipe (17) has passage (18), the top fixedly connected with mixing box (19) of extension board (14), the inner wall bottom of mixing box (19) is top-down's shrink form, the left and right sides symmetry of mixing box (19) is provided with first solenoid valve (32) and second solenoid valve (33), the bottom middle part intercommunication of mixing box (19) has connecting pipe (20), the inside of connecting pipe (20) is provided with the solenoid valve, the bottom of connecting pipe (20) with the top intercommunication of passage (18), the top of mixing box (19) all communicates there is inlet pipe (21), each the top of inlet pipe (21) all communicates there is storage case (22).

2. A cell culture apparatus according to claim 1, wherein: the sliding plates (6) are in a step shape, the step surface of each sliding plate (6) is fixedly connected with the bottom end of each corresponding culture dish (7), the bottom end of each material passing pipe (13) is communicated with an elongated pipe (23), and the elongated pipe (23) is provided with various length specifications.

3. A cell culture assembly according to claim 2, wherein: the middle part of each material passing pipe (13) is fixedly connected with a limiting plate (24), and the bottom end of each limiting plate (24) is connected with the top end of the supporting box (1) through a screw.

4. A cell culture assembly according to claim 3 wherein: the inner wall of the abdicating groove is fixedly connected with a sealing gasket (25).

5. A cell culture apparatus according to claim 4, wherein: the bottom left part and the equal fixedly connected with spacing axle (26) of bottom right part of keysets (4), each the circumference outer wall of spacing axle (26) respectively with sliding fit about the left part and the right part of frame plate (2), the bottom left part fixedly connected with support column (27) of frame plate (2), the bottom of support column (27) with the inner wall bottom fixed connection of supporting box (1).

6. A cell culture assembly according to claim 5 wherein: the outer side wall upper portion fixedly connected with of compounding case (19) widen board (28), the bottom of storage case (22) all with the top fixed connection of widen board (28).

7. A cell culture apparatus according to claim 6, wherein: the inner wall right-hand member fixed connection of supporting box (1) has photoelectric sensor (29), trigger block (30) are installed to the right-hand member of sliding plate (6), the right-hand member mid-mounting of supporting box (1) has control panel (31), photoelectric sensor (29) and electric putter (3) all with control panel (31) electricity is connected.

8. A method of culturing a cell culture apparatus according to any of claims 1 to 7, comprising the steps of:

step one, starting the electric push rod (3), extending and retracting an output shaft of the electric push rod (3), further adjusting the height of the culture dish (7), then extending an output shaft of the rotary clamping cylinder (10), and further driving a cover plate (12) at the upper part of the culture dish (7) to open;

step two, starting the moving module (15), driving the discharging pipe (17) to move left and right by the moving module (15), and stopping the moving module (15) when the discharging pipe (17) moves to the material passing pipe (13) above the culture dish (7) to be fed;

step three, let in each storage case (22) with the cultivation raw materials, the cultivation raw materials lets in mixing box (19) through inlet pipe (21) and mixes, open first solenoid valve (32) and second solenoid valve (33) when it all gathers in first solenoid valve (32) and second solenoid valve (33) department, make the cultivation raw materials pile up in the inside solenoid valve of connecting pipe (20), the rethread opens the inside solenoid valve of connecting pipe (20), nutrient substance lets in through material pipe (13) in passage pipe (18) and discharging pipe (17), let in nutrient substance inside culture dish (7) through passage pipe (13).