CN113521112A - Preparation method of vaginal active probiotic liquid for women - Google Patents
Preparation method of vaginal active probiotic liquid for women Download PDFInfo
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- CN113521112A CN113521112A CN202110982301.9A CN202110982301A CN113521112A CN 113521112 A CN113521112 A CN 113521112A CN 202110982301 A CN202110982301 A CN 202110982301A CN 113521112 A CN113521112 A CN 113521112A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 61
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 61
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 53
- 239000007788 liquid Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 44
- 238000002156 mixing Methods 0.000 claims abstract description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 14
- 238000009835 boiling Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 7
- 239000001888 Peptone Substances 0.000 claims abstract description 7
- 108010080698 Peptones Proteins 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 239000003708 ampul Substances 0.000 claims abstract description 7
- 235000015278 beef Nutrition 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 238000011049 filling Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000002372 labelling Methods 0.000 claims abstract description 7
- 235000019319 peptone Nutrition 0.000 claims abstract description 7
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 7
- 239000011780 sodium chloride Substances 0.000 claims abstract description 7
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 abstract description 8
- 210000001215 vagina Anatomy 0.000 description 11
- 208000004926 Bacterial Vaginosis Diseases 0.000 description 9
- 208000037009 Vaginitis bacterial Diseases 0.000 description 9
- 208000003251 Pruritus Diseases 0.000 description 5
- 230000002159 abnormal effect Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000007803 itching Effects 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000005906 menstruation Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 208000004998 Abdominal Pain Diseases 0.000 description 2
- 206010006784 Burning sensation Diseases 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 208000008350 Pruritus Vulvae Diseases 0.000 description 2
- 206010046914 Vaginal infection Diseases 0.000 description 2
- 201000008100 Vaginitis Diseases 0.000 description 2
- 206010056530 Vulvovaginal pruritus Diseases 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 208000002003 vulvitis Diseases 0.000 description 2
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000606125 Bacteroides Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000052343 Dares Species 0.000 description 1
- 208000004145 Endometritis Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010016326 Feeling cold Diseases 0.000 description 1
- 241000207201 Gardnerella vaginalis Species 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 206010027304 Menopausal symptoms Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204048 Mycoplasma hominis Species 0.000 description 1
- 208000029082 Pelvic Inflammatory Disease Diseases 0.000 description 1
- 208000005107 Premature Birth Diseases 0.000 description 1
- 206010036590 Premature baby Diseases 0.000 description 1
- 206010049677 Salpingo-oophoritis Diseases 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229960002227 clindamycin Drugs 0.000 description 1
- KDLRVYVGXIQJDK-AWPVFWJPSA-N clindamycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@H](C)Cl)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 KDLRVYVGXIQJDK-AWPVFWJPSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229960000282 metronidazole Drugs 0.000 description 1
- VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 125000001446 muramyl group Chemical group N[C@@H](C=O)[C@@H](O[C@@H](C(=O)*)C)[C@H](O)[C@H](O)CO 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
Abstract
The invention discloses a preparation method of vaginal active probiotic liquid for women, which comprises the following steps: s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution; s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank; s3, heating and boiling the medical water, cooling to 35-40 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging. The active probiotic liquid has the characteristic of adapting to the temperature of a human body, is in a liquid state, can be more uniformly dispersed on the surface of the vaginal wall, releases probiotics and active components of the probiotics, and has better effect of adjusting the balance of vaginal flora.
Description
Technical Field
The invention relates to the technical field of preparation of gynecological vaginal external products, in particular to a preparation method of gynecological vaginal active probiotic liquid.
Background
Bacterial Vaginosis (BV) is the most common genital infectious disease of women of childbearing age and is characterized in that the flora balance inhabiting in the vagina is disordered, lactobacillus, particularly a strain producing hydrogen peroxide (H2O2) is reduced, and other flora such as Gardnerella vaginalis, curvulus mobilis, Bacteroides, Streptococcus digestus, mycoplasma hominis and the like are massively propagated to cause a syndrome without inflammation expression of the vaginal mucosa. BV is long in curing time and easy to relapse, has direct relation with a plurality of serious obstetrics and gynecology complications, and can cause the problems of increased premature birth rate of BV patients, low infant physique, tissue villitis, amniotic fluid infection, endometritis and the like. Clinically, BV is mainly treated by adopting antibacterial therapy, and the medicament is preferably selected from antibiotics such as metronidazole, clindamycin and the like, but the cure rate of the two medicaments is only about 60 percent, the recurrence rate is as high as 30 to 40 percent, and the cure rate is in a reduction trend. The use of antibiotics for treating vaginosis not only kills harmful pathogenic bacteria, but also kills probiotic groups beneficial to health, and damages the micro-ecological balance and health of human bodies. Researchers have recently advocated the goal of treating and preventing BV by "regulating vaginal microecological balance", and international studies on vaginal microecological regulation have focused on probiotic studies. The probiotics refer to live bacteria preparation with the functions of improving the ecological balance of host (human and animal) flora and improving the health level of the host. Besides a large amount of active probiotic bacteria, the probiotic bacteria fermentation liquid also contains probiotic metabolites, such as muramyl mucopeptide polysaccharide, probiotic enzyme, short-chain fatty acid, bacteriocin and the like. By implanting the probiotic preparation into the vagina, the normal flora and the acidity of the vagina can be effectively recovered, the resistance of the vagina to the invasion of external harmful bacteria is enhanced, the colonization of the harmful bacteria is inhibited, the self-cleaning effect of the vagina is promoted, and the probiotic preparation has good effects on curing and preventing the recurrence of BV.
At present, the application of active probiotic preparations in gynecological vaginal products is mainly limited by the following factors: 1. exogenous probiotic strains are easily antagonized by the original flora in the vagina and have difficulty in continuous and long-term planting in the vagina; 2. most of active probiotic preparation products on the market are orally taken, however, the live bacteria preparation is easily inactivated by gastric acid and bile after being orally taken, the number of live bacteria is greatly reduced before the live bacteria preparation reaches the intestinal tract, and the bacterial components, the administration times, the treatment course and the like can influence the curative effect of the probiotic preparation; 3. the external preparation of the active probiotic preparation product on the market mainly comprises powder, film agent, tablets, suppositories and the like, and is mainly pushed into or plugged into the vagina or directly coated on the vaginal wound when in use, so that the vaginal preparation is poor in use convenience, and the external preparation on the market is hard, and a patient has strong foreign body sensation when in use; 4. the gynecological vaginal preparation on the market is complex in formula and various in auxiliary materials, insoluble auxiliary materials in the preparation are difficult to discharge in the vagina, and double infection and even multiple infection of patients are easily caused.
Publication No. CN107714672B discloses a vaginal active probiotic gel capsule for women and a preparation method thereof, wherein the preparation method comprises the following steps: culturing active probiotic bacteria liquid, S1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution S2 by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain the active probiotic bacteria liquid; 2. mixing peptone, sodium chloride, beef extract powder, water and agar, dissolving at low temperature, adjusting pH to weak acidity, boiling at 121 deg.C, sterilizing for 20 min, filtering, and packaging in culture tank. S3, heating and boiling the medical water, cooling to 35-40 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging. The product can be uniformly dispersed on the surface of vaginal wall to release probiotics and active ingredients thereof, and regulate vaginal flora balance, thereby preventing or treating bacterial vaginosis; after absorbing the liquid containing the active probiotics, the conduit cotton sliver uniformly disperses the active probiotics on the surface of the vaginal wall in the low-pH environment of the vagina, thus being suitable for market popularization and application. It is in gel state when used, and the dispersion effect on the vaginal wall surface is not particularly uniform when used, and the effect is to be improved.
Disclosure of Invention
The invention provides a preparation method of active probiotic liquid for women, aiming at overcoming the defects in the prior art, the active probiotic liquid prepared by the preparation method has the characteristic of adapting to the temperature of a human body, is liquid, is uniformly dispersed on the surface of the vaginal wall, releases probiotics and active ingredients thereof, regulates the balance of vaginal flora, and further prevents or treats bacterial vaginosis; the carrier is a conduit cotton sliver which is waterproof and flexible, and after the active probiotic liquid is adsorbed and sent to the vagina, the active probiotic can be slowly released in a low-pH environment, and the active probiotic liquid is sent to and extrudes the vaginal wall to release the content, so that the use comfort of a patient can be obviously improved.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention provides a preparation method of vaginal active probiotic liquid for women, which is characterized by comprising the following steps:
s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution;
s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank;
s3, heating and boiling the medical water, cooling to 35-40 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging.
The invention has the beneficial effects that: the vaginal active probiotic liquid for women prepared by the method has the characteristic of adapting to the temperature of a human body, is in a liquid state, can be more uniformly dispersed on the surface of the vaginal wall compared with a gel state, releases probiotics and probiotic active ingredients, and has a better effect of adjusting the balance of vaginal flora.
Detailed Description
The technical solutions of the present invention will be described clearly and completely in the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of vaginal active probiotic liquid for women comprises the following steps:
s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution;
s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank;
s3, heating and boiling the medical water, cooling to 38 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging.
Example 2
A preparation method of vaginal active probiotic liquid for women comprises the following steps:
s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution;
s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank;
s3, heating and boiling the medical water, cooling to 36 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging.
Example 3
A preparation method of vaginal active probiotic liquid for women comprises the following steps:
s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution;
s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank;
s3, heating and boiling the medical water, cooling to 39 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to the mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging.
Examples of the applications
1. Sunxx, age 32, gynecological inflammation, leukorrhagia, abnormal odor, and pruritus vulvae. After the medicine is used for 2 days, peculiar smell does not exist, external pruritus vulvae does not exist within 5 days, all symptoms are basically cured in one week, 3 boxes are used continuously, and the disease is completely cured at present.
2. King xx, the age of 27, moderate cervical erosion, using the product for 3 days, feeling cool below, no scorching sensation of the former, using 3 boxes, detecting by hospitals, and the cervical erosion is cured.
3. Korea xx, age 48, vaginitis, abnormal leucorrhea, vulvitis, adnexitis, itching and odor
The product can be used for 8 days, pruritus and peculiar smell are caused, abnormal leucorrhea is improved, and the product can be used for 3 boxes to completely cure vaginitis, vulvitis and annexitis.
4. Wang xx,32 years old, the stomach feels uncomfortable all the time after the child is born, dares about doing house activities all the time, takes 5 days, discharges more metabolites, takes 10 days, feels the lower body cool, and all the time recovers as before.
5. Tensor XX,56 years old, menopausal for two years. The itching and the peculiar smell are all kept, the sexual intercourse is dared not, brown substances are metabolized after 7 days, the leucorrhea is improved abnormally, and the improvement is completed after 20 days.
6. The product has the advantages of XX, age 49, climacteric, intermittent menstruation, itching peculiar smell, burning sensation, no sitting or sitting every day, disappearance of the burning sensation in two days, complete absence of the itching peculiar smell in 15 days, and much reduction of climacteric symptoms.
7. The product is suitable for people of 26 years old, more than one year in marriage, and after prenuptial abortion, abdominal pain always occurs, menstruation is abnormal every month, many products are used without improvement, the abdominal pain disappears after trial for 20 days, a lot of blood stasis blocks are excreted, and menstruation is normal after 2 months.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (1)
1. A preparation method of vaginal active probiotic liquid for women is characterized by comprising the following steps:
s1, sequentially transferring bifidobacterium and photosynthetic bacteria into a sterile culture solution by using a bacteria transferring needle in a sterilization and disinfection sterile room, and performing high-density culture to obtain an active probiotic bacteria solution;
s2, mixing the components of peptone, sodium chloride, beef extract powder, water and agar, dissolving at a low temperature, adjusting the pH value to be weak acid, boiling at 121 ℃ for sterilizing for 20 minutes, filtering, and subpackaging in a culture tank;
s3, heating and boiling the medical water, cooling to 35-40 ℃, and mixing with the active probiotic bacteria liquid obtained in the step S1 according to a mass ratio of 1: 10, fully and uniformly mixing to obtain active probiotic bacteria-containing liquid, filling the liquid into an ampoule bottle which is sterilized at high temperature in an aseptic workshop, capping, spraying air, labeling and packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110982301.9A CN113521112A (en) | 2021-08-25 | 2021-08-25 | Preparation method of vaginal active probiotic liquid for women |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110982301.9A CN113521112A (en) | 2021-08-25 | 2021-08-25 | Preparation method of vaginal active probiotic liquid for women |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113521112A true CN113521112A (en) | 2021-10-22 |
Family
ID=78092078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110982301.9A Pending CN113521112A (en) | 2021-08-25 | 2021-08-25 | Preparation method of vaginal active probiotic liquid for women |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113521112A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030118571A1 (en) * | 1999-05-25 | 2003-06-26 | Gregor Reid | Oral administration of lactobacillus for the maintenance of health in women during pregnancy and at other life stages, to reduce the risk of urogenital diseases |
| CN112057480A (en) * | 2020-09-29 | 2020-12-11 | 深圳市东荣生物科技有限责任公司 | Microbial preparation for treating vaginitis |
-
2021
- 2021-08-25 CN CN202110982301.9A patent/CN113521112A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030118571A1 (en) * | 1999-05-25 | 2003-06-26 | Gregor Reid | Oral administration of lactobacillus for the maintenance of health in women during pregnancy and at other life stages, to reduce the risk of urogenital diseases |
| CN112057480A (en) * | 2020-09-29 | 2020-12-11 | 深圳市东荣生物科技有限责任公司 | Microbial preparation for treating vaginitis |
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