CN113519523B - Application of polypeptide in preparation of preparation for preventing and treating plant diseases - Google Patents
Application of polypeptide in preparation of preparation for preventing and treating plant diseases Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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Abstract
The invention relates to the technical field of plant disease control, in particular to application of polypeptide in preparing a preparation for preventing and treating plant diseases. The preparation has obvious prevention and treatment effects on plant diseases caused by Erwinia amylovora and has outstanding prevention and treatment effects on the fire blight of rosaceous plants, particularly economic crops such as pears, apples, hawthorns, Chinese flowering crabapples and the like. Compared with the prior art, the polypeptide preparation has the effects of wide action range, high safety, no biotoxicity, no drug resistance, no stimulation and no hormone, and can effectively prevent and treat plant diseases.
Description
Technical Field
The invention relates to the technical field of plant disease control, in particular to application of polypeptide in preparation of a preparation for preventing and treating plant diseases.
Background
Plant disease refers to a phenomenon that plants generate a series of pathological changes in form, physiology and biochemistry under the influence of biological or non-biological factors, and the progress of normal growth and development is blocked, so that the economic benefit of human beings is influenced. Among them, Erwinia amylovora (Erwinia amylovora) causes a fire blight of pear, which is the first established plant bacterial disease. The pear fire blight is a destructive disease, is a dangerous pest for quarantine of imported plants in China, and is an important bacterial disease on fruit trees such as pears, apples and the like in North America.
Erwinia amylovora (Erwinia amylovora), also known as Erwinia amylovora, has a wide host range and can infect more than 220 plants of more than 40 genera in Rosaceae (Van D Z T, Beer S V.fire blank: its nature, preservation, and control: a precursor to integrated disease management [ J ]. agricultural information bulletin (USA),1992.) the distribution range of which includes North America, China, Europe, Western, New Zealand, Africa, etc., can cause serious economic loss, and is a very easy-to-prevent apple and pear tree infectious and devastating bacterial disease in all countries of the world.
Pear fire Blight can damage young shoots, branches, leaves, flowers, and fruits, and leaves are stained dark brown (Heitefrus R.Fire light, History, Biology, and Management [ J ]. Journal of Phytopathology,2012,160 (7-8)). When the disease condition is serious, the disease rapidly spreads from young shoots to branches and trunks until roots, and the whole plant dies. Once infected with the fire blight of pear, it can quickly infect the whole plant and can be transmitted through fruit tree nursery stock and migratory bird in long distance, and insects such as bee, birds and wind and rain can also be the transmission medium, and once infected, the whole orchard, and even the whole peripheral industry can have large area loss, not only, it can also be transmitted through the fruit with bacteria and the polluted packaging material (Hubaishi, xu Shi just, research progress of distribution, transmission and detection technology of the fire blight of pear [ J ]. plant quarantine, 1999(03): 7-11.).
Around the prevention and control of the pear fire blight, a great deal of research is carried out abroad, and prevention and control technologies comprise measures of strengthening quarantine, pruning and eradicating diseased plants, preventing and controlling medicaments, screening disease-resistant varieties, biologically preventing and controlling, improving the disease resistance of fruit trees by using a gene engineering technology and the like. However, the disease control difficulty is high, no specific medicament or single control measure exists so far, the disease is not well controlled, and the disease control method still is a main concern of pear and apple producing areas. The method for preventing and treating the pear fire blight at the present stage mainly aims to strengthen agricultural prevention and treatment, strictly quarantine, prohibit introduction of rosaceous fruit tree seedlings in an affected area, and screen resistant plant varieties, and simultaneously carry out medicament prevention and treatment, and mainly sprays Bordeaux mixture or agricultural streptomycin, neomycin, tetramycin and 14% cuaminosulfate water aqua. At present, agricultural streptomycin is the main pesticide for preventing and treating pear fire blight in the United states, and kasugamycin is used in areas with drug resistance.
The concentration of Bordeaux mixture and 14% cuaminosulfate aqua cannot be too high, so that phytotoxicity is easy to occur, streptomycin sulfate is a common antibiotic for human and livestock, and long-term accumulation on the whole biological chain can cause super bacteria with drug resistance, so that the survival of human beings is threatened. Kasugamycin, tetramycin and neophytycin are antibiotics which are not common antibiotics for human and livestock but are antibiotics, and are easy to generate drug resistance.
Along with the generation of phenomena such as drug resistance and the like, people turn the direction of preventing and treating the pear fire blight to biological control, and a streptomyces lydicus strain and application thereof in the control of the fire blight are disclosed in a patent with the application number of 201910266800.0; application No. 201711363218.3 discloses the use of a strain of myxococcus phyllum preying on plant pathogenic bacteria for the prevention and treatment of Erwinia amylovora (Erwinia amylovora).
In connection with pear-resistant genetic transformation, studies by Gully K et al indicate that prococop 12 is an endogenous peptide that shows a higher tolerance to pear fire blight-induced cell death (Gully K, Pelletier S, Guliou MC, et al. the SCOOP12peptide ligands for responses and root infection in Arabidopsis thaliana [ J ]. Journal of Experimental Botany, Vol.70, No.4 pp.1349-1365,2019), which in experiments transformed prococop 12 to obtain mutant plants; zhangyu and other researches show that the pear fire blight resistant transgenic apple tree can effectively improve the resistance of plants to fire blight (Zhangyu, Liu Rev, Dinghanfeng, Biyuping. the research progress of antibacterial peptide and the application in agriculture [ J ] Anhui agricultural science, 2006(03): 433-. Therefore, the transfer of the antibacterial peptide gene into crops is an effective way for cultivating disease-resistant varieties. The method is complex to operate, and resistant plants can be obtained only by multiple steps of transformation, screening, culture and the like, and meanwhile, no method for preventing and treating the pear fire blight by directly acting the antibacterial peptide on the plants is mentioned.
Certainly, a few reports that the polypeptide has an effect on amylovorax are disclosed, but the antibacterial peptide represents a basic polypeptide substance with antibacterial activity, and the antibacterial spectrum of the antibacterial peptide with the same sequence length also has specificity, for example, the research of Esther Badosa and the like shows that BP77 and BP100 are synthesized linear undecapeptides, and BP77 has no effect on the pear fire blight and BP100 has a better prevention and treatment effect on the pear fire blight. (EatherBadosa, Rafael Ferre, Marta plates, Lidia Feliu, Emili Besal u, Jordi Cabrefgia, Eduard Bardaj I, Emilio Montesino. A library of linear undecapeptides with bacterial activity against bacterial bacteria [ J ]. Peptides,2007,28 (12)), and an antimicrobial peptide with an APD ID of AP02249 and a sequence length of 11, which has an antibacterial effect only against gram-positive bacteria and MRSA (methicillin-resistant Staphylococcus aureus) and has no bactericidal effect against gram-negative bacteria, and Erwinia amylolytica belongs to gram-negative bacteria and has no bactericidal effect against Erwinia amylolytica. For another example, the sequence length of the antibacterial peptide with the APD ID of AP00546 in the APD antibacterial peptide database is 19, but in the antibacterial aspect, the antibacterial peptide has an effect on gram-positive bacteria and malaria and no effect on gram-negative bacteria, while the sequence length of the polypeptide of the present invention is 19, which is different from the above in the antibacterial spectrum range, so it can be seen that different sequence lengths, even polypeptides with the same sequence length, have different amino acid composition sequences, which result in different functions, and different peptides have different characteristics, and the antibacterial spectrum of the different peptides needs to be researched according to their own characteristics, so there is no referential property between different peptides.
The applicant finds that the polypeptide has obvious prevention and treatment effects on the pear fire blight in research, and the prior art does not disclose that the polypeptide can be used for preventing and treating plant diseases.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the application of the polypeptide in preparing a preparation for preventing and treating plant diseases. Meanwhile, a polypeptide preparation for preventing and treating plant diseases is provided, which is characterized in that:
the preparation comprises a polypeptide with an amino acid sequence of SEQ ID NO.1 or a preparation prepared by taking the polypeptide as an active ingredient and auxiliary materials or auxiliary ingredients.
In addition, a method for preventing and treating plant diseases is also provided.
The polypeptide preparation can be any one or combination of external solid preparation, semi-solid preparation or liquid preparation.
Preferably, the auxiliary components comprise common agents for plant diseases.
The application of the polypeptide in preparing the preparation for preventing and treating the plant diseases is characterized in that the polypeptide preparation can be used for preventing and treating the plants with the plant diseases.
The plant disease is caused by Erwinia amylovora (Erwinia amylovora).
The plant is a Rosaceae plant, wherein the Rosaceae plant is preferably a pear, an apple, a hawthorn or a crabapple.
The invention provides a polypeptide preparation for preventing and treating plant diseases, which is characterized in that the concentration of polypeptide is 0.004g/L-1 g/L.
The invention provides a method for preventing and treating plant diseases, which comprises the following steps of contacting any one of the following plant parts in the using process: seeds, roots, stems, leaves or fruits, or contact with soil or any other production medium surrounding the plant's roots.
Preferably, the polypeptide preparation is contacted with the diseased site of the plant or applied to the soil at the root of the plant.
The invention provides an application of polypeptide in preparing a preparation for preventing and treating plant diseases, which can effectively prevent and kill Erwinia amylovora.
Drawings
FIG. 1 is a graph showing the effect of MIC test of polypeptides on Erwinia amylovora.
FIG. 2 is a graph showing the therapeutic effect of control group 2 on greenhouse Pyrus betulaefolia seedlings and polypeptides.
Detailed Description
The invention provides application of a polypeptide in preparing a preparation for preventing and treating plant diseases, which is characterized in that the amino acid sequence of the polypeptide is SEQ ID NO. 1.
The invention provides a preparation containing polypeptide with an amino acid sequence of SEQ ID NO. 1.
The invention provides a preparation which is prepared by taking polypeptide with an amino acid sequence of SEQ ID NO.1 as an active ingredient and auxiliary materials or auxiliary ingredients.
The invention also provides a method for preventing and treating plant diseases.
The Erwinia amylovora XJSZ0102 provided by Zhejiang university.
The polypeptide of the present invention can be obtained by chemical synthesis, or by expression, separation and purification by genetic engineering techniques (see Sambrook et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2001).
Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and the like added in the examples are commercially available unless otherwise specified.
The polypeptide preparation can be any one or combination of external solid preparation, semi-solid preparation or liquid preparation.
The auxiliary materials can be one or a combination of more of conventional auxiliary materials in preparations such as sucrose, starch, dextrin, magnesium stearate, sorbitol, mannitol, lactose, microcrystalline cellulose, aerosil, carboxymethyl cellulose, water and the like, and the specific selection of which auxiliary material or materials is determined according to the required preparation formulation.
The auxiliary component refers to a preparation which can improve the sterilization effect of the polypeptide or can also prevent and treat plant diseases, such as Bordeaux mixture and the like, and preferably, the auxiliary component is a common medicament for the plant diseases, such as kasugamycin, zhongshengmycin, ethylicin, limonene and the like.
The invention also provides application of the polypeptide preparation in preventing and treating plant diseases, which is characterized in that the polypeptide preparation is used for preventing and treating plants with the plant diseases.
The plant disease is caused by Erwinia amylovora (Erwinia amylovora).
The plant is a Rosaceae plant, and preferably, the Rosaceae plant is pear, apple, hawthorn or crabapple.
The polypeptide preparation for preventing and treating plant diseases is prepared by contacting any one of the following plant parts in the using process: seeds, roots, stems, leaves or fruits, or any other production medium surrounding the plant's roots. Preferably, the polypeptide preparation is contacted with the diseased site of the plant or applied to the soil at the root of the plant.
This section of the examples further illustrates the content of the invention but should not be construed as limiting the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention.
Example 1 determination of the Effect of different concentrations of the polypeptide on Erwinia amylovora
The net content concentration of the selected polypeptide is 1.000g/L, 0.500g/L, 0.100g/L, 0.050g/L, 0.010g/L, 0.005g/L and 0.001 g/L.
Plate streaking is carried out on the Erwinia amylovora XJSZ0102, and a single colony on a fresh plate is picked up and cultured in 3mL LB liquid culture medium at 27 ℃ and 200rpm for 36 h. Adjusting the OD600 to 0.4 +/-0.01, and diluting by 10000 times to obtain a standby bacterium. When in use, the final concentration of the bacteria is 106CFU/mL。
And (4) preparing a neutralizing agent. 10mL system: 0.2g of lecithin is aseptically weighed, added with 300 mu L of absolute ethyl alcohol for dissolution, added with 9.5mL of LB liquid culture medium and 200 mu L of Tween-80, and shaken and evenly stirred. The actions are all finished in the super clean bench.
Preparation of sample set: taking 1mL of polypeptide, adding 100 μ L of the standby bacterial liquid into 1.5mL of EP tube, shaking uniformly, and standing for 10 min. After 10min, 100 mu L of the solution is added into 900 mu L of the neutralization reagent to stop the reaction, and after the solution is continuously diluted by 10 times, 100 mu L of the solution is coated on a flat plate for detection.
Preparation of control group: 1mL of sterile water is taken to be put into a 1.5mL EP tube, 100 mu L of standby bacterial liquid is added, and the mixture is shaken uniformly and then is kept stand for 10 min. After 10min, 100. mu.L of the solution is added into 900. mu.L of the neutralizing agent, and the solution is continuously diluted by 100 times and then 100. mu.L of the solution is coated on a flat plate for detection.
The results are shown in Table 1.
TABLE 1 statistics of the antibacterial effect of different polypeptide concentrations on Erwinia amylovora
| Concentration of polypeptide | Number of colonies per plate | Mean value/one | Rate of sterilization |
| 1.000g/L | 0/0/0 | 0 | 100.00％ |
| 0.500g/L | 0/0/0 | 0 | 100.00％ |
| 0.100g/L | 0/0/0 | 0 | 100.00％ |
| 0.050g/L | 0/0/0 | 0 | 100.00％ |
| 0.010g/L | 0/0/0 | 0 | 100.00％ |
| 0.005g/L | 0/0/0 | 0 | 100.00％ |
| 0.001g/L | 57/64/52 | 57.67 | 84.55％ |
| Control group | 33/37/42 | 37.33 | - |
As shown in Table 1, the content of the polypeptide in the concentration range of 0.005g/L to 1g/L showed that the content of the polypeptide in Erwinia amylovora was 100%. Therefore, the polypeptide has stronger bactericidal action on the Erwinia amylovora.
Example 2 MIC experiment of polypeptide against Erwinia amylovora
NB sterile medium containing 64mg/L polypeptide was prepared and diluted to 32mg/L, 16mg/L, 8mg/L, 4mg/L, 2mg/L in NB medium fold by fold, 400. mu.L of medium was added to 96-well plates per concentration, 200. mu.L was added per well. Adjusting the concentration of the activated Erwinia amylovora XJSZ0102 to 1 × 106cfu/ml 10. mu.L per well of bacterial suspension was prepared. And simultaneously setting a positive control containing bacteria and no polypeptide and a blank control containing no bacteria and no polypeptide. Placing the mixture in a constant temperature shaking incubator at 28 ℃ and 150rpm for culturing for 48 h.
The results are shown in FIG. 1.
The positive control and 2mg/L polypeptide group grew bacteria, and the other groups grew aseptically.
The results of the MIC experiment show that: when the effective concentration of the polypeptide reaches 4mg/L, the higher bacteriostatic efficiency on the Erwinia amylovora can be achieved, and the polypeptide has stronger capacity of killing the Erwinia amylovora.
Example 3 fungicidal Effect on greenhouse Pyrus pyrifolia seedlings
Preparing bacterial liquid, streaking and activating Erwinia amylovora XJSZ0102 stored in a refrigerator at-70 ℃ on an NA plate culture medium, culturing in an incubator (28 ℃ and dark) for 48h, picking out a single colony, inoculating in an NB liquid culture medium, and performing shaking culture at 28 ℃ and 150rpm for 24 h.
Preparing polypeptide concentration, namely preparing polypeptide liquid with the concentration of 1g/L by using sterile water for standby.
Agricultural streptomycin is a main medicament for preventing and controlling the pear fire blight disease, and the preventing and controlling effect of the agricultural streptomycin on the pear fire blight disease is superior to that of other medicaments, so that the agricultural streptomycin is compared with the sterilizing effect of polypeptide on Erwinia amylovora of greenhouse birchleaf pear seedlings. The concentration of streptomycin for agricultural use in this experiment was 0.25 g/L.
The medicament treatment and the spray inoculation are adopted, the inoculation scheme of the experiment adopts the spray inoculation, and the inoculation concentration is 1 multiplied by 109CFU/mL. The birch pear seedlings with basically consistent growth vigor are selected and divided into two groups: a prevention group (spraying bacteria first and then spraying bacteria) and a treatment group (spraying bacteria first and then spraying bacteria) are arranged, and a control group 1 (spraying sterile water for inoculation) and a control group 2 (spraying sterile water for inoculation) are arranged. One replicate for each 10 plants. Spraying and inoculating bacteria 24 hours after the prevention group is sprayed with the pesticide, and laminating and moisturizing the bacteria 72 hours after inoculation; spraying and inoculating bacteria to the treatment groups, covering films, moisturizing for 24 hours, and spraying.
The statistical method is to periodically observe the disease occurrence condition of the test seedlings and count the total number of branches, the number of diseased branches and the disease occurrence length of the branches. The drug effect of the medicament is preliminarily judged according to the morbidity, and the disease index is calculated according to the morbidity length of the branches.
The formula for calculating the morbidity, disease index and relative prevention and treatment effect is as follows:
the criteria for the grade of disease are shown in Table 2.
TABLE 2 grade criteria
| Grade value of disease | Grade standard of disease |
| Level 0 | No disease symptoms |
| Level 1 | The disease area accounts for less than 1/3 of the total branch length |
| Grade 3 | The affected area has a total branch length of 1/3-2/3 |
| Grade 5 | The affected area has a total branch length of 2/3 or more |
After 15 days of inoculation, statistics is carried out on the plant disease conditions, and the experimental results are shown in tables 3 and 4.
TABLE 3 preventive Effect of the Polypeptides on greenhouse potted seedlings
TABLE 4 therapeutic Effect of the Polypeptides on greenhouse potted seedlings
The therapeutic effect of control 2 and the polypeptide is shown in FIG. 2.
As can be seen from tables 3 and 4, the incidence rate of the polypeptide in the prevention group to the pear fire blight is 2.94%, while the incidence rate of the control group 1 is 8.29%, and the incidence rate is 2.82 times of that of the polypeptide in the prevention group; the incidence of the polypeptide in the treatment group to the pear fire blight is 6.81 percent, while the incidence of the control group 1 is 17.99 percent, and the incidence of the polypeptide in the treatment group is 2.64 times that of the polypeptide in the treatment group, so that the polypeptide has obvious effects on preventing and treating the pear fire blight.
The incidence of the agricultural streptomycin on the pear fire blight in the prevention group is 1.97%, and the incidence of the agricultural streptomycin on the pear fire blight in the treatment group is 11.44%.
According to the calculation formula, the disease index statistical analysis and the relative prevention and treatment effect are carried out on each experimental group, and the analysis results are shown in table 5.
TABLE 5 greenhouse potted seedling chemical prevention and control and disease index and relative prevention and control effect
As can be seen from Table 5, the disease index of the polypeptide is obviously lower than that of the control group 1 in both the prevention group and the treatment group, the relative prevention effect of the polypeptide reaches 72.8 percent, and the relative treatment effect reaches 66.5 percent, and the polypeptide is verified to have good prevention and treatment effects on the pear fire blight again.
Meanwhile, the experimental result shows that the prevention effect of the streptomyces agriculturally is slightly higher than that of the polypeptide in the prevention group, the treatment effect of the streptomyces agriculturally is obviously lower than that of the polypeptide in the treatment group, and the treatment effect of the polypeptide is obviously higher than that of the streptomyces agriculturally although the prevention effect of the polypeptide is slightly lower than that of the streptomyces agriculturally.
Agricultural streptomycin has a good effect of preventing and treating the pear fire blight in the existing medicament, but is an agricultural antibiotic, can be enriched in a human body through a food chain, so that an accumulative risk is generated, and drug resistance is easy to appear, and is forbidden by the nation at present.
The polypeptide of the invention is composed of 19 amino acids, has no toxicity and no irritation, the degradation product is natural amino acid, and no drug residue is generated, meanwhile, the sterilization mechanism of the polypeptide is that the polypeptide carries strong positive charges to be adsorbed to the surface of the thallus, so that the local potential of the cell wall suddenly changes to form high potential difference, and finally the cell wall is punctured to form perforation, and when the polypeptide molecules accumulate to a certain amount, the formed holes are further enlarged, the integrity of the cell membrane is damaged, and therefore, the substance in the cell flows out, and the thallus dies. The unique sterilization mechanism determines that the polypeptide is not easy to generate drug resistance.
The polypeptide has the advantages of obviously higher treatment effect than that of streptomyces agriculturally used, slightly lower prevention effect than that of streptomycin agriculturally used, obvious advantages in the aspect of preventing and treating plant diseases based on the characteristics of no toxicity, no stimulation, no biological toxicity and no drug resistance generation, and wide application prospect.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Sequence listing
<110> Jiangsu Jia peptide Biotechnology GmbH
<120> application of polypeptide in preparation of preparation for preventing and treating plant diseases
<141> 2020-04-13
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 19
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 1
Met Gly Arg Phe Lys Arg Phe Arg Lys Lys Phe Lys Lys Leu Phe Lys
1 5 10 15
Lys Leu Ser
Claims (15)
1. The application of the polypeptide in preparing the preparation for preventing and treating plant diseases is characterized in that the amino acid sequence of the polypeptide is SEQ ID NO. 1.
2. Use according to claim 1, characterized in that the plant disease is caused by Erwinia amylovora (Erwinia amylovora).
3. The use according to any one of claims 1-2, wherein said polypeptide formulation is in the form of a combination of one or more of a solid, semi-solid or liquid formulation for external use.
4. Use according to claim 3, characterized in that the plant is a Rosaceae plant.
5. Use according to claim 4, characterized in that the Rosaceae plant is a pear, apple, hawthorn or crabapple.
6. An application of polypeptide preparation containing amino acid sequence SEQ ID NO.1 in preventing and treating plant diseases is provided.
7. Use according to claim 6, characterized in that the concentration of the polypeptide is between 0.004g/L and 1 g/L.
8. The use according to claim 6 or 7, wherein the polypeptide formulation is in the form of a solid, semi-solid or liquid formulation for external application.
9. The use of claim 8, wherein the preparation is prepared from the polypeptide with the amino acid sequence of SEQ ID NO.1 as an active ingredient and auxiliary materials or auxiliary ingredients.
10. Use according to claim 9, characterized in that the auxiliary ingredients comprise agents commonly used for plant diseases.
11. A method for preventing and treating plant diseases, characterized in that a polypeptide preparation according to any one of claims 7, 9 and 10 is contacted with a plant infected with Erwinia amylovora (Erwinia amylovora).
12. A method for preventing and treating plant diseases characterized by contacting a plant infected with Erwinia amylovora (Erwinia amylovora) with the polypeptide preparation of claim 8.
13. The method according to claim 11, wherein said preparation of polypeptides is contacted with any one of the following plant parts: seeds, roots, stems, leaves or fruits, or contact soil or any other production medium surrounding the plant's roots.
14. The method according to claim 12, wherein said preparation of polypeptides is contacted with any one of the following plant parts: seeds, roots, stems, leaves or fruits, or contact soil or any other production medium surrounding the plant's roots.
15. The method according to claim 13 or 14, characterized in that the polypeptide preparation is brought into contact with the diseased part of the plant or is applied to the root soil of the plant.
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