CN113514428A - Photoelectric sensing laser-induced fluorescence detection device - Google Patents

Photoelectric sensing laser-induced fluorescence detection device Download PDF

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Publication number
CN113514428A
CN113514428A CN202010280888.4A CN202010280888A CN113514428A CN 113514428 A CN113514428 A CN 113514428A CN 202010280888 A CN202010280888 A CN 202010280888A CN 113514428 A CN113514428 A CN 113514428A
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China
Prior art keywords
laser
plate
fixed
wall
sensing laser
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CN202010280888.4A
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Chinese (zh)
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CN113514428B (en
Inventor
刘超
张秋月
刘佳琳
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Nanjing Ruilanshi Photoelectric Sensor Technology Research Institute Co ltd
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Nanjing Ruilanshi Photoelectric Sensor Technology Research Institute Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6402Atomic fluorescence; Laser induced fluorescence
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16MFRAMES, CASINGS OR BEDS OF ENGINES, MACHINES OR APPARATUS, NOT SPECIFIC TO ENGINES, MACHINES OR APPARATUS PROVIDED FOR ELSEWHERE; STANDS; SUPPORTS
    • F16M11/00Stands or trestles as supports for apparatus or articles placed thereon ; Stands for scientific apparatus such as gravitational force meters
    • F16M11/02Heads
    • F16M11/04Means for attachment of apparatus; Means allowing adjustment of the apparatus relatively to the stand
    • F16M11/043Allowing translations
    • F16M11/045Allowing translations adapted to left-right translation movement
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16MFRAMES, CASINGS OR BEDS OF ENGINES, MACHINES OR APPARATUS, NOT SPECIFIC TO ENGINES, MACHINES OR APPARATUS PROVIDED FOR ELSEWHERE; STANDS; SUPPORTS
    • F16M11/00Stands or trestles as supports for apparatus or articles placed thereon ; Stands for scientific apparatus such as gravitational force meters
    • F16M11/02Heads
    • F16M11/18Heads with mechanism for moving the apparatus relatively to the stand
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation

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  • General Engineering & Computer Science (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention belongs to the technical field of laser-induced fluorescence detection, and particularly relates to a photoelectric sensing laser-induced fluorescence detection device which comprises a fixed seat, wherein a supporting frame is fixedly arranged at the top of the fixed seat, an installation frame is fixedly arranged at the top of the supporting frame, a laser, a dichroic mirror and a focusing lens are fixedly arranged in the installation frame from top to bottom in sequence, an adjusting groove is formed in the top of the fixed seat, a moving plate is connected to the inner wall of the bottom of the adjusting groove in a sliding manner, and a limiting assembly is fixedly arranged on one side of the moving plate; the invention has convenient and fast operation, molecules in the biological sample can generate fluorescence reaction under the excitation of laser, the identification of molecules with different biological properties and sources can be realized by measuring the frequency, the amplitude and the service life of the fluorescence, and meanwhile, the position of the detection box can be adjusted in the using process, thereby having convenient and fast operation and stronger practicability.

Description

Photoelectric sensing laser-induced fluorescence detection device
Technical Field
The invention relates to the technical field of laser-induced fluorescence detection, in particular to a photoelectric sensing laser-induced fluorescence detection device.
Background
The essence of biological behavior is the movement of proteins and molecules, and in the complex matrix constituting human or other organisms, the composition and behavior of the molecules can be detected in real time and non-invasively by spectroscopic analysis, and the molecules with specific spectroscopic characteristics have diagnostic value. Molecules in the biological sample can perform a fluorescence reaction under the excitation of laser, and the identification of the molecules with different biological properties and sources can be realized by measuring the frequency, amplitude and service life of fluorescence. The big data analysis and artificial intelligence technology are combined with the classical physics technology, and real-time and high-flux detection and analysis can be realized.
The existing laser-induced fluorescence detection device is complex to operate in the actual application process, meanwhile, a detection point is fixed, the detection position cannot be adjusted transversely according to the use requirement, and laser ray emission deviation cannot be achieved, so that the photoelectric sensing laser-induced fluorescence detection device is provided, and the problem is solved.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a photoelectric sensing laser-induced fluorescence detection device.
In order to achieve the purpose, the invention adopts the following technical scheme:
a photoelectric sensing laser-induced fluorescence detection device comprises a fixed seat, wherein a support frame is fixedly mounted at the top of the fixed seat, a mounting frame is fixedly mounted at the top of the support frame, a laser, a dichroic mirror and a focusing lens are sequentially and fixedly mounted in the mounting frame from top to bottom, an adjusting groove is formed in the top of the fixed seat, a moving plate is connected to the inner wall of the bottom of the adjusting groove in a sliding manner, a limiting assembly is fixedly mounted at one side of the moving plate, a rack is fixedly mounted at the other side of the moving plate, a rotating shaft is rotatably mounted on the inner wall of one side of the adjusting groove, a gear is fixedly sleeved on the outer side of the rotating shaft and meshed with the rack, a fixed box is fixedly mounted at one end of the rotating shaft, an elastic resetting assembly is arranged in the fixed box, a pushing shaft is fixedly mounted on the elastic resetting assembly and once penetrates through the inner wall of one side of the adjusting groove and extends to one side of the fixed seat, and promote axle and the vertical sliding connection of fixing base, the one end fixed mounting who promotes the axle has the button, be provided with on the fixing base and be used for the braking subassembly to the button braking, the top fixed mounting of movable plate has the backup pad, the top fixed mounting of backup pad has the detection case, fixed mounting has two optical detection probe that the symmetry set up in the detection case, the top of detection case is rotated and is articulated to have the apron, the top fixed mounting of fixing base has the spectrum appearance, spectrum appearance and optical detection probe electric connection.
Preferably, the bottom four corners of the fixing seat are fixedly provided with supporting legs, the bottom of each supporting leg is fixedly provided with a sucker, and the fixing seat can be fixed at a designated position by the aid of the suckers.
Preferably, the bottom fixed mounting of movable plate has the slider, fixed mounting has the slide rail on the bottom inner wall of adjustment tank, and slider and slide rail sliding connection utilize slider and slide rail can make the movable plate remain firm when lateral shifting, and can not take place the skew.
Preferably, spacing subassembly includes the spacing seat of fixed mounting in movable plate one side, sliding connection has the gag lever post in the spacing seat, the one end of gag lever post and one side inner wall fixed connection of adjustment tank utilize gag lever post and spacing seat to keep firm when can be so that movable plate lateral shifting, and can not take place the skew.
Preferably, the elastic reset assembly comprises a supporting spring fixedly mounted on the inner wall of the top of the fixed box, a sliding plate is fixedly mounted at the bottom of the supporting spring, the sliding plate is connected with the inner walls of the two sides of the fixed box in a sliding manner, the top of the pushing shaft extends into the fixed box and is fixedly connected with the bottom of the sliding plate, when the button is pressed again, the button can push the pushing shaft, the pushing shaft can press the supporting spring to deform, the pushing shaft can bear reverse pushing force, and when the button is loosened, the button can be reset under the reset elastic force of the supporting spring.
Preferably, the braking component comprises two clamping grooves which are respectively formed in two sides of the button, two symmetrically-arranged supporting rods are fixedly mounted on one side of the fixing seat, clamping plates are fixedly mounted at one ends of the supporting rods and are respectively clamped with the two clamping grooves, and the button can be braked under the action of the clamping plates and the clamping grooves.
Preferably, the cover plate is provided with a circular through hole, and the radiation generated by the laser can be emitted into the detection box by utilizing the circular through hole.
According to the invention, when a biological sample is required to be detected by using the laser-induced fluorescence detection device body, the cover plate is opened at the moment, then the biological sample is placed in the detection box, the laser is started at the moment, most of laser generated by laser dust is refracted onto the focusing lens through the dichroic mirror, the laser can be focused by the focusing lens and irradiates the biological sample in the detection box through the through hole, the irradiated laser on the medical sample can be detected and collected by using the optical detection probe, the collected laser is emitted into the spectrometer, and the specific spectral characteristics generated by the biological sample are analyzed by using the spectrometer.
When the user demand according to the fluorescence detection device body to the detection case lateral adjustment position when needs, press the button this moment, the button can promote to promote the axle and remove, when draw-in groove and cardboard phase separation on the button, alright with the braking to the button of having removed, can rotate the button this moment, the button can drive the gear and rotate, and gear and rack mesh mutually, so can drive the movable plate and carry out lateral shifting, and the movable plate can drive the detection case and carry out lateral shifting, and then can adjust the lateral position of detection case, convenient operation is swift, has stronger practicality.
The invention has convenient and fast operation, molecules in the biological sample can generate fluorescence reaction under the excitation of laser, the identification of molecules with different biological properties and sources can be realized by measuring the frequency, the amplitude and the service life of the fluorescence, and meanwhile, the position of the detection box can be adjusted in the using process, thereby having convenient and fast operation and stronger practicability.
Drawings
FIG. 1 is a schematic view of an overall structure of a photoelectric sensing laser-induced fluorescence detection apparatus according to the present invention;
FIG. 2 is a schematic cross-sectional view of a portion of the fixing base shown in FIG. 1 of the device for detecting fluorescence induced by a photoelectric sensor laser according to the present invention;
FIG. 3 is an enlarged schematic structural diagram of a portion A in FIG. 1 of a photo-sensor laser-induced fluorescence detection apparatus according to the present invention;
FIG. 4 is a schematic top view of a portion of the FIG. 1 of a photo-sensor laser-induced fluorescence detection apparatus according to the present invention;
FIG. 5 is a schematic top view of a portion of the FIG. 4 of the optoelectronic sensing laser induced fluorescence detection apparatus according to the present invention;
fig. 6 is a schematic top view of the cover plate in fig. 1 of the photo-sensor laser-induced fluorescence detection apparatus according to the present invention.
In the figure: the spectrometer comprises a fixed seat 1, a support frame 2, a mounting frame 3, a laser 4, a dichroic mirror 5, a focusing lens 6, a support plate 7, a detection box 8, an optical detection probe 9, a cover plate 10, a through hole 11, an adjusting groove 12, a movable plate 13, a limiting seat 14, a limiting rod 15, a rack 16, a rotating shaft 17, a gear 18, a fixed box 19, a support spring 20, a sliding plate 21, a push shaft 22, a button 23, a support rod 24, a clamping plate 25, a clamping groove 26 and a spectrometer 27.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1-6, a photoelectric sensing laser-induced fluorescence detection device comprises a fixed base 1, a support frame 2 is fixedly installed on the top of the fixed base 1, an installation frame 3 is fixedly installed on the top of the support frame 2, a laser 4, a dichroic mirror 5 and a focusing lens 6 are fixedly installed in the installation frame 3 from top to bottom in sequence, an adjustment groove 12 is formed in the top of the fixed base 1, a moving plate 13 is connected onto the inner wall of the bottom of the adjustment groove 12 in a sliding manner, a limiting assembly is fixedly installed on one side of the moving plate 13, a rack 16 is fixedly installed on the other side of the moving plate 13, a rotating shaft 17 is rotatably installed on the inner wall of one side of the adjustment groove 12, a gear 18 is fixedly sleeved on the outer side of the rotating shaft 17, the gear 18 is meshed with the rack 16, a fixed box 19 is fixedly installed at one end of the rotating shaft 17, an elastic resetting assembly is arranged in the fixed box 19, a pushing shaft 22 is fixedly installed on the elastic resetting assembly, promote one side inner wall of axle 22 in case of running through adjustment tank 12 and extend to one side of fixing base 1, and promote axle 22 and the vertical sliding connection of fixing base 1, the one end fixed mounting who promotes axle 22 has button 23, be provided with on the fixing base 1 and be used for the braking subassembly to button 23, the top fixed mounting of movable plate 13 has backup pad 7, the top fixed mounting of backup pad 7 has detection case 8, fixed mounting has two optical detection probe 9 that the symmetry set up in the detection case 8, the top of detection case 8 is rotated and is articulated to have apron 10, the top fixed mounting of fixing base 1 has spectrum appearance 27, spectrum appearance 27 and optical detection probe 9 electric connection.
Example two
The embodiment is improved on the basis of the first embodiment: the four corners of the bottom of the fixed seat 1 are fixedly provided with supporting legs, the bottoms of the supporting legs are fixedly provided with suckers, and the fixed seat 1 can be fixed at a specified position by utilizing the suckers; the bottom of the moving plate 13 is fixedly provided with a slide block, the inner wall of the bottom of the adjusting groove 12 is fixedly provided with a slide rail, and the slide block and the slide rail are in sliding connection, so that the moving plate 13 can be kept stable and stable when moving transversely by the slide block and the slide rail, and can not deviate; the limiting assembly comprises a limiting seat 14 fixedly installed on one side of the moving plate 13, a limiting rod 15 is connected in the limiting seat 14 in a sliding mode, one end of the limiting rod 15 is fixedly connected with the inner wall of one side of the adjusting groove 12, and the moving plate 13 can be kept stable and stable when moving transversely and cannot deviate by means of the limiting rod 15 and the limiting seat 14; the elastic reset assembly comprises a support spring 20 fixedly mounted on the inner wall of the top of the fixed box 19, a sliding plate 21 is fixedly mounted at the bottom of the support spring 20, the sliding plate 21 is in sliding connection with the inner walls of the two sides of the fixed box 19, the top of the push shaft 22 extends into the fixed box 19 and is fixedly connected with the bottom of the sliding plate 21, when the button 23 is pressed, the push shaft 22 can be pushed by the button 23, the support spring 20 can be pressed by the push shaft 22 to deform, and then the push shaft 22 bears reverse pushing force, and when the button 23 is released, the button 23 can be reset under the reset elastic force of the support spring 20; the braking component comprises two clamping grooves 26 which are respectively arranged at two sides of the button 23, two symmetrically arranged supporting rods 24 are fixedly arranged at one side of the fixed seat 1, one end of each supporting rod 24 is fixedly provided with a clamping plate 25, the two clamping plates 25 are respectively clamped with the two clamping grooves 26, and the button 23 can be braked under the action of the clamping plates 25 and the clamping grooves 26; the cover plate 10 is provided with a circular through hole, and the radiation generated by the laser 4 can be emitted into the detection box 8 by using the circular through hole.
In the invention, when a biological sample needs to be detected by using the laser-induced fluorescence detection device body, the cover plate 10 is opened at the moment, then the biological sample is placed in the detection box 8, the laser 4 is started at the moment, most of laser generated by dust of the laser 4 is refracted onto the focusing lens 6 through the dichroic mirror 5, the focusing lens 6 can focus the laser and irradiates the biological sample in the detection box 8 through the through hole 11, the irradiated laser on the medical sample can be detected and collected by using the optical detection probe 9, the collected laser is emitted into the spectrometer 27 at the same time, and the specific spectral features generated by the biological sample are analyzed by using the spectrometer 27.
When the user demand according to fluorescence detection device body is to detection case 8 lateral adjustment position, press button 23 this moment, button 23 can promote to promote and promote axle 22 and remove, when draw-in groove 26 and cardboard 25 phase separation on button 23, alright have been solved the braking to button 23, can rotate button 23 this moment, button 23 can drive gear 18 and rotate, and gear 18 and rack 16 mesh mutually, so can drive movable plate 13 and carry out lateral shifting, and movable plate 13 can drive detection case 8 and carry out lateral shifting, and then can adjust detection case 8's lateral position, and convenient operation is swift, has stronger practicality.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims (7)

1. The utility model provides a photoelectric sensing laser-induced fluorescence detection device, includes fixing base (1), its characterized in that, the top fixed mounting of fixing base (1) has support frame (2), the top fixed mounting of support frame (2) has mounting bracket (3), from last to having laser instrument (4), dichroic mirror (5) and focusing lens (6) to fixed mounting in proper order down in mounting bracket (3), adjustment tank (12) have been seted up at the top of fixing base (1), sliding connection has movable plate (13) on the bottom inner wall of adjustment tank (12), one side fixed mounting of movable plate (13) has spacing subassembly, the opposite side fixed mounting of movable plate (13) has rack (16), rotate on one side inner wall of adjustment tank (12) and install rotation axis (17), the fixed cover in the outside of rotation axis (17) is equipped with gear (18), and the gear (18) is meshed with the rack (16), one end of the rotating shaft (17) is fixedly provided with a fixed box (19), an elastic reset assembly is arranged in the fixed box (19), a push shaft (22) is fixedly arranged on the elastic reset assembly, the push shaft (22) penetrates through the inner wall of one side of the adjusting groove (12) and extends to one side of the fixed seat (1), the push shaft (22) is connected with the fixed seat (1) in a longitudinal sliding manner, one end of the push shaft (22) is fixedly provided with a button (23), the fixed seat (1) is provided with a brake assembly for braking the button (23), the top of the movable plate (13) is fixedly provided with a support plate (7), the top of the support plate (7) is fixedly provided with a detection box (8), and the detection box (8) is internally fixedly provided with two optical detection probes (9) which are symmetrically arranged, the top of detection case (8) is rotated to articulate and is had apron (10), the top fixed mounting of fixing base (1) has spectrum appearance (27), spectrum appearance (27) and optical detection probe (9) electric connection.
2. The device for detecting fluorescence induced by photoelectric sensing laser according to claim 1, wherein the four corners of the bottom of the fixing base (1) are fixedly provided with legs, and the bottom of each leg is fixedly provided with a sucker.
3. The photo-electric sensing laser-induced fluorescence detection device according to claim 1, wherein a slide block is fixedly mounted at the bottom of the moving plate (13), a slide rail is fixedly mounted on the inner wall of the bottom of the adjusting groove (12), and the slide block and the slide rail are connected in a sliding manner.
4. The photoelectric sensing laser-induced fluorescence detection device according to claim 1, wherein the limiting assembly comprises a limiting seat (14) fixedly installed on one side of the moving plate (13), a limiting rod (15) is slidably connected in the limiting seat (14), and one end of the limiting rod (15) is fixedly connected with an inner wall on one side of the adjusting groove (12).
5. The photo-electric sensing laser-induced fluorescence detection device according to claim 1, wherein the elastic reset assembly comprises a support spring (20) fixedly installed on the inner wall of the top of the fixed box (19), a sliding plate (21) is fixedly installed on the bottom of the support spring (20), the sliding plate (21) is slidably connected with the inner walls of the two sides of the fixed box (19), and the top of the pushing shaft (22) extends into the fixed box (19) and is fixedly connected with the bottom of the sliding plate (21).
6. The device for detecting fluorescence induced by photoelectric sensing laser according to claim 1, wherein the brake assembly comprises two clamping grooves (26) respectively formed at two sides of the button 23, two symmetrically arranged support rods (24) are fixedly installed at one side of the fixing base (1), one end of each support rod (24) is fixedly installed with a clamping plate (25), and the two clamping plates (25) are respectively clamped with the two clamping grooves (26).
7. The device for detecting fluorescence induced by photoelectric sensing laser according to claim 1, wherein the cover plate (10) is provided with a circular through hole.
CN202010280888.4A 2020-04-10 2020-04-10 Photoelectric sensing laser-induced fluorescence detection device Active CN113514428B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114720471A (en) * 2022-02-21 2022-07-08 河源市蓝海米克模具刀具有限公司 Cutter passivation detection system and detection method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103196879A (en) * 2013-03-20 2013-07-10 上海如海光电科技有限公司 Laser-induced fluorescence spectrum detection device
CN104483297A (en) * 2014-12-08 2015-04-01 上海通微分析技术有限公司 Visualization calibration laser-induced fluorescence detection device
CN206281740U (en) * 2016-12-06 2017-06-27 广州番禺职业技术学院 A kind of new distribution type ultrasonic slurry density precision measuring instrument
CN212008324U (en) * 2020-04-10 2020-11-24 南京瑞蓝世光电传感技术研究院有限公司 Photoelectric sensing laser-induced fluorescence detection device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103196879A (en) * 2013-03-20 2013-07-10 上海如海光电科技有限公司 Laser-induced fluorescence spectrum detection device
CN104483297A (en) * 2014-12-08 2015-04-01 上海通微分析技术有限公司 Visualization calibration laser-induced fluorescence detection device
CN206281740U (en) * 2016-12-06 2017-06-27 广州番禺职业技术学院 A kind of new distribution type ultrasonic slurry density precision measuring instrument
CN212008324U (en) * 2020-04-10 2020-11-24 南京瑞蓝世光电传感技术研究院有限公司 Photoelectric sensing laser-induced fluorescence detection device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114720471A (en) * 2022-02-21 2022-07-08 河源市蓝海米克模具刀具有限公司 Cutter passivation detection system and detection method thereof
CN114720471B (en) * 2022-02-21 2022-12-30 河源市蓝海米克模具刀具有限公司 Cutter passivation detection system and detection method thereof

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