CN113508807B - Ovarian normal-temperature preservation solution and preservation method thereof - Google Patents
Ovarian normal-temperature preservation solution and preservation method thereof Download PDFInfo
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- CN113508807B CN113508807B CN202110897917.6A CN202110897917A CN113508807B CN 113508807 B CN113508807 B CN 113508807B CN 202110897917 A CN202110897917 A CN 202110897917A CN 113508807 B CN113508807 B CN 113508807B
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- 239000003761 preservation solution Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004321 preservation Methods 0.000 title claims abstract description 15
- 230000002611 ovarian Effects 0.000 title claims description 15
- 210000001672 ovary Anatomy 0.000 claims abstract description 55
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 239000008103 glucose Substances 0.000 claims abstract description 18
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 claims abstract description 14
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 claims abstract description 14
- 229940110767 coenzyme Q10 Drugs 0.000 claims abstract description 14
- 235000017471 coenzyme Q10 Nutrition 0.000 claims abstract description 14
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 10
- 229940088710 antibiotic agent Drugs 0.000 claims abstract description 10
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003637 basic solution Substances 0.000 claims abstract description 8
- 239000011777 magnesium Substances 0.000 claims abstract description 8
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 8
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 20
- 229930182555 Penicillin Natural products 0.000 claims description 10
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 10
- 229940049954 penicillin Drugs 0.000 claims description 10
- 229960005322 streptomycin Drugs 0.000 claims description 10
- 230000003115 biocidal effect Effects 0.000 claims 1
- 230000003325 follicular Effects 0.000 abstract description 8
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 16
- 241000272525 Anas platyrhynchos Species 0.000 description 13
- 239000000243 solution Substances 0.000 description 10
- 206010002091 Anaesthesia Diseases 0.000 description 6
- 206010015719 Exsanguination Diseases 0.000 description 6
- 230000037005 anaesthesia Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 5
- 230000029087 digestion Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- YRQNKMKHABXEJZ-UVQQGXFZSA-N chembl176323 Chemical compound C1C[C@]2(C)[C@@]3(C)CC(N=C4C[C@]5(C)CCC6[C@]7(C)CC[C@@H]([C@]7(CC[C@]6(C)[C@@]5(C)CC4=N4)C)CCCCCCCC)=C4C[C@]3(C)CCC2[C@]2(C)CC[C@H](CCCCCCCC)[C@]21C YRQNKMKHABXEJZ-UVQQGXFZSA-N 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 210000002394 ovarian follicle Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of biology and provides a biological enzyme,in particular to an ovary normal-temperature preservation solution and a preservation method thereof, the ovary normal-temperature preservation solution takes normal saline as basic solution and is added with glucose and Mg2+Coenzyme Q10 and antibiotics. The concentration of the glucose in the ovary normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 was 10 μmol/L. The invention can solve the problem that the number and the quality of the follicular cells are greatly reduced when the follicular cells are preserved at normal temperature, the preservation time at normal temperature is about 22 to 37 days, and the high-quality follicular cells are obtained.
Description
Technical Field
The invention relates to the field of biology, and in particular relates to a normal-temperature ovarian preservation solution and a preservation method thereof.
Background
At present, the maximum time for storing the ovary at normal temperature is only about 8 hours, and the ovary is too urgent for the condition of needing long-distance transportation or field collection. The quantity and quality of the follicular cells which can be collected within 8 hours are greatly reduced, and the great waste of the follicular cells is caused.
Disclosure of Invention
In order to solve the problems, the invention provides an ovarian normal-temperature preservation solution and a preservation method thereof, which can obtain high-quality ovarian follicle cells.
In order to achieve the purpose, the invention adopts the technical scheme that:
an ovary normal temperature preservation solution, which takes normal saline as a base solution and is added with glucose and Mg2+Coenzyme Q10 and antibiotics.
Further, the concentration of the glucose in the ovarian normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 was 10 μmol/L.
Further, the antibiotics are penicillin and streptomycin, the concentration of the penicillin is 0.07 mg/mL, and the concentration of the streptomycin is 0.06 mg/mL.
The invention also provides an ovary normal-temperature preservation method of the ovary normal-temperature preservation solution, which comprises the following steps of:
washing ovary with preheated PBS solution for 3 times, rinsing with sterilized normal saline for 1 time, and placing in sterilized ovary normal temperature preservation solution.
Further, the ovary preservation temperature is 10-20 ℃.
Further, 50-60 ovaries per liter of ovary normal-temperature preservation solution are preserved.
The invention has the following beneficial effects:
can solve the problem that the quantity and the quality of the follicular cells are greatly reduced when the follicular cells are stored at normal temperature, the storage time at normal temperature is about 22 to 37 days, and the follicular cells with high quality are obtained.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 15 ℃; the ovary normal temperature preservation solution takes normal saline as basic solution, and is added with glucose and Mg2+Coenzyme Q10 and antibiotics, wherein the concentration of the glucose in the ovarian normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 is 10 mu mol/L, the concentration of penicillin is 0.07 mg/mL, and the concentration of streptomycin is 0.06 mg/mL.
Example 2
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 20 ℃; the ovary normal temperature preservation solution takes normal saline as basic solution, and is added with glucose and Mg2+Coenzyme Q10 and antibiotics, wherein the concentration of the glucose in the ovarian normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 is 10 mu mol/L, the concentration of penicillin is 0.07 mg/mL, and the concentration of streptomycin is 0.06 mg/mL.
Comparative example 1
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 15 ℃; the ovary normal temperature preservation solution takes normal saline as basic solution, and is added with glucose and Mg2+And antibiotics, wherein the concentration of the glucose in the ovarian normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of penicillin was 0.07 mg/mL and the concentration of streptomycin was 0.06 mg/mL.
Comparative example 2
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 15 ℃; the normal-temperature ovary preservation solution takes normal saline as basic solution and is added with glucose, coenzyme Q10 and antibiotics, wherein the concentration of the glucose in the normal-temperature ovary preservation solution is 2 mmol/L; the concentration of coenzyme Q10 is 10 mu mol/L, the concentration of penicillin is 0.07 mg/mL, and the concentration of streptomycin is 0.06 mg/mL.
Comparative example 3
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 15 ℃; the normal-temperature ovary preservation solution takes normal saline as basic solution and is added with glucose and antibiotics, wherein the concentration of the glucose in the normal-temperature ovary preservation solution is 2 mmol/L; the concentration of penicillin was 0.07 mg/mL and the concentration of streptomycin was 0.06 mg/mL.
Comparative example 4
A normal-temperature ovary preservation method comprises the following steps: randomly selecting a female duck, performing anesthesia treatment, then performing neck exsanguination and sacrifice, quickly taking out the whole ovary, cleaning for 3 times by using preheated PBS (phosphate buffer solution), rinsing for 1 time by using sterilized normal saline, placing the female duck in the ovary normal-temperature preservation solution after the sterilization treatment, and preserving at 15 ℃; the ovary normal temperature preservation solution takes normal saline as basic solution, and is added with glucose and Mg2+Coenzyme Q10 and antibiotics, wherein the concentration of the glucose in the ovarian normal-temperature preservation solution is 16 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 is 10 mu mol/L, the concentration of penicillin is 0.07 mg/mL, and the concentration of streptomycin is 0.06 mg/mL.
And (3) comparison test:
the ovaries obtained in example 1, example 2, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 were treated with the following procedure: recovering ovary at 25 deg.C for 20min, transferring ovary into 35 deg.C PBS solution to separate ovary medulla, washing ovary cortex with clean PBS solution, washing ovary cortex with 70% ethanol twice (10S each time), washing with normal saline 2 times to remove blood stain and excessive connective tissue, homogenizing in calcium and magnesium ion-free PBS solution at 3600rpm for 6min, centrifuging, and removing supernatant. Transferring the filtered tissue homogenate into an enzymolysis solution (150 IU/mLDNase I +0.75mg/mL collagenase I + alpha-MEM), digesting for 40-60min in a 37 ℃ water bath with shaking, stopping digestion with a digestion stopping solution (PBS +10% chicken serum) after digestion is finished, repeatedly blowing the solution by using a bus pipette to fully release the solution containing the follicle, separating the follicle to obtain a follicle suspension, and then washing the follicle with the PBS solution for multiple times under a stereomicroscope to suck out the follicles (primary follicle, primary follicle and secondary follicle) at each stage.
The longest time for which the ovaries of example 1, example 2, comparative example 1, comparative example 2, comparative example 3, and comparative example 4 can be preserved was measured while maintaining the standard without decreasing based on the number of follicles at each stage and histological characteristics of follicles of fresh duck ovaries. And (3) test results: example 1: 37 days, example 2:22 days, comparative example 1: 18 days, comparative example 2: 18 days, comparative example 3: 0.35 days, comparative example 4: for 32 days.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (5)
1. An ovarian normal-temperature preservation solution is characterized in that: the ovary normal temperature preservation solution takes normal saline as basic solution, and is added with glucose and Mg2+Coenzyme Q10 and antibiotics; the concentration of the glucose in the ovary normal-temperature preservation solution is 2 mmol/L; mg (magnesium)2+The concentration of (A) is 15 mmol/L; the concentration of coenzyme Q10 was 10 μmol/L.
2. The normal-temperature ovarian preservation solution as claimed in claim 1, wherein the normal-temperature ovarian preservation solution comprises the following components: the antibiotic is penicillin and streptomycin, the concentration of the penicillin is 0.07 mg/mL, and the concentration of the streptomycin is 0.06 mg/mL.
3. The method for preserving ovarian at room temperature according to claim 1, wherein the method comprises the following steps: the method comprises the following steps:
removing fat tissue around ovary, rinsing ovary with sterilized normal saline for 3 times, and placing in sterilized ovary normal temperature preservation solution.
4. The method for preserving ovarian at room temperature according to claim 1, wherein the method comprises the following steps: the ovary preservation temperature is 10-20 ℃.
5. The method for preserving ovarian at room temperature according to claim 1, wherein the method comprises the following steps: 50-60 ovaries are preserved in the ovary normal-temperature preservation solution per liter.
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| CN115606578A (en) * | 2022-09-08 | 2023-01-17 | 吉林省农业科学院 | Preserving fluid for prolonging in-vitro preserving time of cow ovary |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2501337A1 (en) * | 2004-03-18 | 2005-09-18 | Arindom Sen | Chemical dissociation of cell aggregates |
| CN103190391A (en) * | 2013-03-28 | 2013-07-10 | 金�一 | Method for improving integrity of cytoskeletons after freeze thawing of preserved boar sperm |
| CN107376022A (en) * | 2017-06-27 | 2017-11-24 | 浙江大学 | A kind of ovary in natural tissues source takes off cell material and preparation method thereof |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9804774D0 (en) * | 1998-03-07 | 1998-04-29 | Glacier Metal Co Ltd | Plain bearing |
| US6153582A (en) * | 1998-11-05 | 2000-11-28 | Bausch & Lomb Surgical, Inc. | Defined serumfree medical solution for ophthalmology |
| US6579866B2 (en) * | 2000-12-28 | 2003-06-17 | Mccleary Larry | Composition and method for modulating nutrient partitioning |
| WO2012061754A2 (en) * | 2010-11-05 | 2012-05-10 | The Broad Institute, Inc. | Compounds and methods for treating autoimmune diseases |
| KR20130141719A (en) * | 2010-11-12 | 2013-12-26 | 알러간, 인코포레이티드 | Metabolized conditioned growth medium and methods of use |
| CN103444700B (en) * | 2013-09-04 | 2015-06-10 | 重庆市畜牧科学院 | Simple in-vitro ovary storage method |
| CN104719285B (en) * | 2015-04-16 | 2016-08-31 | 中国农业科学院特产研究所 | Ovary room-temperature extender and store method |
| WO2018093918A1 (en) * | 2016-11-15 | 2018-05-24 | Biologica Technologies | Tissue implants and uses thereof |
| BR112020008519A2 (en) * | 2017-10-30 | 2021-01-26 | Abs Global, Inc. | compositions and methods for improving the viability and function of the gamete |
| RU2678106C2 (en) * | 2018-06-08 | 2019-01-23 | Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр радиологии" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ радиологии" Минздрава России) | Method of vitrification of ovarial tissue |
| CN110923200B (en) * | 2019-12-25 | 2021-08-03 | 佛山辅康生物科技有限公司 | Sperm culture solution and preparation method thereof |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2501337A1 (en) * | 2004-03-18 | 2005-09-18 | Arindom Sen | Chemical dissociation of cell aggregates |
| CN103190391A (en) * | 2013-03-28 | 2013-07-10 | 金�一 | Method for improving integrity of cytoskeletons after freeze thawing of preserved boar sperm |
| CN107376022A (en) * | 2017-06-27 | 2017-11-24 | 浙江大学 | A kind of ovary in natural tissues source takes off cell material and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 小鼠卵巢颗粒细胞的体外培养;王妍等;《西北农林科技大学学报(自然科学版)》;20070825(第08期);第19-22页 * |
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