CN113484114B - Be applied to blood sample processor of Parkinson's disease SNCA genotyping - Google Patents
Be applied to blood sample processor of Parkinson's disease SNCA genotyping Download PDFInfo
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- CN113484114B CN113484114B CN202110760449.8A CN202110760449A CN113484114B CN 113484114 B CN113484114 B CN 113484114B CN 202110760449 A CN202110760449 A CN 202110760449A CN 113484114 B CN113484114 B CN 113484114B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2835—Movement disorders, e.g. Parkinson, Huntington, Tourette
Abstract
The invention discloses a blood sample processor applied to SNCA genotyping of Parkinson's disease, which comprises a machine body, an anticoagulant tube storage rack, a high-speed centrifuge, a refrigerating chamber, an enzyme label instrument and a serum adding device, wherein the refrigerating chamber is positioned at the bottom of the machine body, a storage box is arranged at one side of the top of the machine body, the anticoagulant tube storage rack, the high-speed centrifuge and the enzyme label instrument are positioned in the storage box, and the serum adding device is positioned at the other side of the top of the machine body. According to the invention, the storage box is arranged on the machine body and used for storing the anticoagulant tube storage rack, the high-speed centrifugal machine and the enzyme label instrument, and all the equipment is concentrated on the machine body to form integrated equipment, so that the experiment operation is convenient; through setting up the serum interpolation device on the organism, conveniently fix the experimental needle, improved the stability of experimental needle, made things convenient for the injection of serum, improved the accuracy of experiment.
Description
Technical Field
The invention relates to the technical field of blood analysis, in particular to a blood sample processor applied to SNCA genotyping of Parkinson's disease.
Background
Parkinson's Disease (PD) is a type of neurodegenerative disease that is mainly symptomatic of motor dysfunction. The core pathology alters the formation of lewy bodies caused by abnormal aggregation of alpha-Synuclein (SNCA). The method leads to degeneration of a nigrostriatal pathway, and causes heterogeneous regulation disorder of a brain network, and is particularly critical for defining pathogenesis, realizing early warning, driving target intervention and the like in view of complex pathogenesis of PD, hidden pathological changes and various clinical characterizations, so that systematic study is carried out in coordination with common problems in the multidisciplinary fields of PD-related genetics, neuroscience, image genetics, nuclear medicine and the like.
The general procedure for blood analysis for PD patients is: the method comprises a series of steps of blood sampling, high-speed blood centrifugation, blood refrigeration, sequencing electrophoresis experiments, serum adding and the like, and the used experiment machines are various, so that the experiment machines are separated and discharged in the current laboratory, are not centralized enough, and are inconvenient to use.
Disclosure of Invention
The invention aims to provide a blood sample processor applied to SNCA genotyping of Parkinson's disease, which integrates experimental equipment into an integrated device to solve the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a be applied to blood sample processor of Parkinson's disease SNCA genotyping, includes organism, anticoagulation pipe storage frame, high-speed centrifuge, walk-in, enzyme-labeling appearance and serum interpolation device, the walk-in is located the bottom of organism, top one side of organism is equipped with the bin, anticoagulation pipe storage frame, high-speed centrifuge, enzyme-labeling appearance all are located the inside of bin, serum interpolation device is located the top opposite side of organism.
Preferably, the storage box is internally provided with three groups of sliding rails corresponding to the anticoagulation tube storage rack, the high-speed centrifugal machine and the enzyme labeling instrument, and the three groups of sliding rails are internally connected with a supporting plate with a positioning assembly in a sliding manner.
Preferably, the locating component is including seting up in the spring groove of backup pad outside limit portion, the inside activity grafting in spring groove has T type locating lever, the other end of T type locating lever is equipped with compression spring, and the inboard of slide rail seted up with T type locating lever assorted locating hole, the tip of T type locating lever sets up to arc structure, and the limit portion of locating hole is provided with the groove.
Preferably, the positioning assembly further comprises a limiting block fixedly connected to the inner side edge of the supporting plate, a limiting groove matched with the limiting block is formed in the inner side of the sliding rail, and the limiting block is movably connected to the inside of the limiting groove.
Preferably, the limit block is provided with a groove at the edge, and the universal ball is mounted in the groove and is connected in the limit groove in a rolling way.
Preferably, the anticoagulation tube storage rack comprises a step type base, an anticoagulation tube placing hole is formed in the top of the step type base, an elastic sheet is fixedly connected to the inner side wall of the anticoagulation tube placing hole, and an arc clamping plate is fixedly connected to the end portion of the elastic sheet.
Preferably, a placing groove is formed in the top of the supporting plate corresponding to the anticoagulant tube storage rack, a magnet mounting groove is formed in the surface of the placing groove and the bottom of the step base, and a magnet is bonded in the magnet mounting groove.
Preferably, the serum adding device comprises an operation platform, the side part of the operation platform is fixedly connected with a U-shaped supporting seat with a threaded hole, a rotating shaft is rotatably connected in the U-shaped supporting seat, the end part of the rotating shaft is fixedly connected with a sleeve, the inner thread of the threaded hole is connected with a rubber head screw, and the end part of the rubber head screw is propped against the end part of the rotating shaft.
Preferably, an opening is formed in the top of the sleeve, and a thumb wheel is rotatably mounted in the opening.
In summary, the beneficial effects of the invention are as follows due to the adoption of the technology:
according to the invention, the storage box is arranged on the machine body and used for storing the anticoagulant tube storage rack, the high-speed centrifugal machine and the enzyme label instrument, and all the equipment is concentrated on the machine body to form integrated equipment, so that the experiment operation is convenient; through setting up the serum interpolation device on the organism, conveniently fix the experimental needle, improved the stability of experimental needle, made things convenient for the injection of serum, improved the accuracy of experiment.
Drawings
FIG. 1 is a schematic diagram of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 2 is a schematic diagram showing the structure of a storage tank of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 3 is a schematic diagram showing the structure of a support plate of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 4 is an enlarged schematic view of the structure of the region A in FIG. 3 of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 5 is a schematic view showing a sectional structure of a step-type base of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 6 is a schematic view of a U-shaped support base structure of a blood sample processor for SNCA genotyping of Parkinson's disease according to the present invention;
FIG. 7 is a schematic diagram showing the structure of a limiting block of a blood sample processor for SNCA genotyping of Parkinson's disease.
In the figure: 1. a body; 2. an anticoagulant tube storage rack; 3. a high-speed centrifuge; 4. a refrigerating chamber; 5. an enzyme-labeled instrument; 6. serum adding means; 7. a storage box; 8. a slide rail; 9. a support plate; 10. a spring groove; 11. a T-shaped positioning rod; 12. a compression spring; 13. positioning holes; 14. a step base; 15. an anticoagulant tube placement hole; 16. a spring plate; 17. an arc clamping plate; 18. a placement groove; 19. a magnet mounting groove; 20. a magnet; 21. a limiting block; 22. a limit groove; 23. a groove; 24. a universal ball; 25. an operating platform; 26. a U-shaped supporting seat; 27. a rotating shaft; 28. a sleeve; 29. a rubber head screw; 30. an opening; 31. a thumb wheel.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
Example 1
The invention provides a blood sample processor applied to SNCA genotyping of Parkinson's disease, which is shown in figure 1, and comprises a machine body 1, an anticoagulant tube storage rack 2, a high-speed centrifuge 3, a refrigerating chamber 4, an enzyme label instrument 5 and a serum adding device 6, wherein the refrigerating chamber 4 is positioned at the bottom of the machine body 1, one side of the top of the machine body 1 is provided with a storage box 7, the anticoagulant tube storage rack 2, the high-speed centrifuge 3 and the enzyme label instrument 5 are all positioned in the storage box 7, the serum adding device 6 is positioned at the other side of the top of the machine body 1, the anticoagulant tube storage rack 2 is used for storing an anticoagulant tube with a blood sample and is convenient to take, the high-speed centrifuge 3 is used for centrifuging the blood sample, the refrigerating chamber 4 is used for placing the blood sample, and the enzyme label instrument 5 is used for measuring absorbance at a wavelength of 450 nm; the serum adding device 6 is used for fixing an experimental needle, and serum is conveniently injected.
Example 2
As shown in fig. 2 to 7, in this embodiment, unlike embodiment 1, there is: the storage box 7 is internally provided with three groups of sliding rails 8 corresponding to the anticoagulation pipe storage rack 2, the high-speed centrifugal machine 3 and the enzyme label instrument 5, the three groups of sliding rails 8 are internally connected with the supporting plate 9 in a sliding manner, the supporting plate 9 is positioned through the positioning assembly, the storage box 7 is provided with a box door with a lock catch, the anticoagulation pipe storage rack 2, the high-speed centrifugal machine 3 and the enzyme label instrument 5 are placed on the supporting plate 9 and stored in the storage box 7, equipment is convenient to store, and the supporting plate 9 and the sliding rails 8 are matched, so that the equipment is convenient to move out of the storage box 7 for use;
the positioning assembly comprises a spring groove 10 formed in the outer side edge of the supporting plate 9, a T-shaped positioning rod 11 is movably inserted into the spring groove 10, a compression spring 12 is arranged at the other end of the T-shaped positioning rod 11, a positioning hole 13 matched with the T-shaped positioning rod 11 is formed in the inner side of the sliding rail 8, when the supporting plate 9 is moved into the storage box 7, one end of the T-shaped positioning rod 11 can be extruded by the compression spring 12 in the spring groove 10 to be inserted into the positioning hole 13, the supporting plate 9 can be fixed, the supporting plate is prevented from sliding out, when the supporting plate 9 needs to be moved out, the T-shaped positioning rod 11 is pulled to be separated from the positioning hole 13, the end part of the T-shaped positioning rod 11 is in an arc structure, and the edge part of the positioning hole 13 is in a groove structure, so that the T-shaped positioning rod 11 can be conveniently inserted into the positioning hole 13;
the positioning assembly further comprises a limiting block 21 fixedly connected to the inner side edge of the supporting plate 9, a limiting groove 22 matched with the limiting block 21 is formed in the inner side of the sliding rail 8, the limiting block 21 is movably connected to the inside of the limiting groove 22, the position of the supporting plate 9 in the sliding rail 8 is limited by the cooperation of the limiting block 21 and the limiting groove 22, the falling phenomenon caused when the supporting plate 9 is pulled is avoided, a groove 23 is formed in the edge of the limiting block 21 and used for installing a universal ball 24, the universal ball 24 is connected to the inside of the limiting groove 22 in a rolling mode, and friction force between the limiting block 21 and the limiting groove 22 can be reduced by the arrangement of the universal ball 24;
in this embodiment, the anticoagulant tube storage rack 2 is provided with a stepped structure, so that the anticoagulant tube storage rack is mainly used for conveniently layering to prevent anticoagulant tubes, can be clearly classified, is convenient to take, is provided with an anticoagulant tube placement hole 15 at the top of each layer of the stepped base 14, is used for preventing anticoagulant tubes, is provided with a label area at each layer of side edge, and is used for preventing labels, in practical application, an elastic sheet 16 can be fixedly connected to the inner side wall of the anticoagulant tube placement hole 15, an arc clamping plate 17 is fixedly connected to the end part of the elastic sheet 16, and after the anticoagulant tubes are placed in the anticoagulant tube placement hole 15, the elastic sheet 16 can squeeze the arc clamping plate 17 to clamp the anticoagulant tubes, so that the stability of the anticoagulant tubes in the anticoagulant tube placement hole 15 is improved; the top of the supporting plate 9 corresponding to the anticoagulation tube storage rack 2 is provided with the placing groove 18 for placing the step-shaped base 14, so that the position of the anticoagulation tube storage rack 2 on the supporting plate 9 can be limited, the anticoagulation tube storage rack is prevented from sliding, the surface of the placing groove 18 and the bottom of the step-shaped base 14 are both provided with the magnet mounting groove 19 for mounting the magnet 20, and the anticoagulation tube storage rack 2 can be fixed through the adsorption effect between the magnets 20.
The serum adding device 6 comprises an operation platform 25, the side part of the operation platform 25 is fixedly connected with a U-shaped supporting seat 26 with a threaded hole, the threaded hole penetrates through the U-shaped supporting seat 26 and is connected with a rubber head screw 29, the end part of the rubber head screw 29 abuts against the end part of a rotating shaft 27, the rubber head screw 29 is mainly used for fixing the rotating shaft 27, accordingly the angle of the sleeve 28 can be fixed, the rotating shaft 27 is connected in the U-shaped supporting seat 26 in a rotating mode, the sleeve 28 is used for mounting the sleeve 28, the size of the sleeve is matched with that of an experiment needle, when serum is injected, the experiment needle is inserted into the sleeve 28, an opening 30 is formed in the top of the sleeve 28, a thumb wheel 31 is rotatably mounted in the opening 30, when the experiment needle is inserted into the sleeve 28, the experiment needle is inserted into the sleeve 28 in a clinging mode, the experiment needle is firstly inserted into the sleeve 28 in a rotating mode, the sleeve 28 is then rotated to adjust the angle of the experiment needle, the rubber head screw 29 abuts against the rotating shaft 27, the angle is fixed, then the thumb wheel 31 drives the experiment needle to move in the sleeve 28, the experiment needle to be conveniently fixed in the sleeve 28, the experiment needle is injected, the experiment needle is convenient to fix, the experiment needle, the stability is improved, and the accuracy of the serum is improved.
Example 3
In the analysis of blood sample treatment using the apparatus of examples 1 and 2 for SNCA gene of Parkinson's disease, subjects (PD group, HC group) meeting the inclusion criteria were subjected to subgroup analysis based on the SNCA susceptibility gene locus measurement result: the at-risk allele carrying group (carrying group, mutant: AG/GG type), the at-risk allele not carrying group (protective group, wild: AA type).
SNCA genotyping and protein expression assays
The PD patients and HC test are put into groups, 10mL of morning fasting elbow normal vein blood sample is extracted one by one, and is put into an ethylenediamine tetraacetic acid anticoagulation tube, 2000g/min is carried out under the condition of 2-8 ℃ of a high-speed constant-temperature centrifuge, the centrifugation is carried out for 10 minutes, and the blood plasma is taken and stored in a refrigerator at-80 ℃;
the serum total SNCA protein and phosphorylated SNCA protein levels of the subject are detected using an enzyme-linked immunosorbent assay. (1) Coating the microplate with purified human SNCA antibody to prepare a solid phase antibody; (2) Sequentially adding serum into the micropores coated with the antibody, and then combining with SNCA antibody marked by horseradish peroxidase to form an antibody-antigen-enzyme-labeled antibody complex; (3) Adding substrate tetramethyl benzidine for color development after thorough washing (chemical reaction is carried out under catalysis so as to be converted into blue, and the color is converted into final yellow under the action of acid, and the color shade is positively correlated with SNCA protein in a sample); (4) Absorbance was measured at 450nm wavelength using an enzyme-labeled instrument, and total SNCA protein, phosphorylated SNCA protein concentration in the sample was measured by standard curve technique.
Genotyping assay: (1) Firstly, designing different specific primers, and performing polymerase chain amplification reaction to obtain a gene fragment containing a mutation site to be detected; (2) performing a multiplex ligase detection reaction; (3) Carrying out sequencing electrophoresis experiments of fragments by a gene sequencer; (4) Data analysis and spot reading were performed by GeneMapper software.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Claims (4)
1. Be applied to blood sample processor of Parkinson's disease SNCA genotyping, including organism (1), anticoagulation pipe storage frame (2), high-speed centrifuge (3), walk-in (4), enzyme-labeling appearance (5) and serum interpolation device (6), its characterized in that: the refrigerating chamber (4) is positioned at the bottom of the machine body (1), a storage box (7) is arranged at one side of the top of the machine body (1), the anticoagulant tube storage rack (2), the high-speed centrifugal machine (3) and the enzyme-labeling instrument (5) are all positioned in the storage box (7), and the serum adding device (6) is positioned at the other side of the top of the machine body (1);
three groups of sliding rails (8) are arranged in the storage box (7) corresponding to the anticoagulation tube storage rack (2), the high-speed centrifugal machine (3) and the enzyme label instrument (5), and the three groups of sliding rails (8) are internally and slidably connected with a supporting plate (9) with a positioning assembly;
the positioning assembly comprises a spring groove (10) formed in the outer side edge of the supporting plate (9), a T-shaped positioning rod (11) is movably inserted into the spring groove (10), a compression spring (12) is arranged at the other end of the T-shaped positioning rod (11), a positioning hole (13) matched with the T-shaped positioning rod (11) is formed in the inner side of the sliding rail (8), the end part of the T-shaped positioning rod (11) is of an arc-shaped structure, and a groove is formed in the edge part of the positioning hole (13);
the positioning assembly further comprises a limiting block (21) fixedly connected to the inner side edge of the supporting plate (9), a limiting groove (22) matched with the limiting block (21) is formed in the inner side of the sliding rail (8), and the limiting block (21) is movably connected to the inside of the limiting groove (22);
a groove (23) is formed in the edge of the limiting block (21), a universal ball (24) is arranged in the groove (23), and the universal ball (24) is connected in the limiting groove (22) in a rolling mode;
the serum adding device (6) comprises an operation platform (25), a U-shaped supporting seat (26) with a threaded hole is fixedly connected to the edge of the operation platform (25), a rotating shaft (27) is rotatably connected to the inside of the U-shaped supporting seat (26), a sleeve (28) is fixedly connected to the end portion of the rotating shaft (27), a rubber head screw (29) is connected to the inner thread of the threaded hole, and the end portion of the rubber head screw (29) abuts against the end portion of the rotating shaft (27).
2. A blood sample processor for use in the genotyping of parkinson's disease SNCA according to claim 1, wherein: anticoagulation pipe storage frame (2) include step base (14), anticoagulation pipe place hole (15) have been seted up at the top of step base (14), and the inside wall fixedly connected with shell fragment (16) of anticoagulation pipe place hole (15), the tip fixedly connected with arc splint (17) of shell fragment (16).
3. A blood sample processor for use in the genotyping of parkinson's disease SNCA according to claim 2, wherein: a placing groove (18) is formed in the top of the supporting plate (9) corresponding to the anticoagulation tube storage rack (2), a magnet mounting groove (19) is formed in the surface of the placing groove (18) and the bottom of the step-type base (14), and a magnet (20) is bonded in the magnet mounting groove (19).
4. A blood sample processor for use in the genotyping of parkinson's disease SNCA according to claim 1, wherein: an opening (30) is formed in the top of the sleeve (28), and a thumb wheel (31) is rotatably arranged in the opening (30).
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| CN202110760449.8A CN113484114B (en) | 2021-07-06 | 2021-07-06 | Be applied to blood sample processor of Parkinson's disease SNCA genotyping |
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| CN113484114B true CN113484114B (en) | 2024-03-05 |
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| WO2018090030A2 (en) * | 2016-11-14 | 2018-05-17 | Siemens Healthcare Diagnostics Inc. | Sample preparation device |
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| US5769811A (en) * | 1995-10-31 | 1998-06-23 | Haemonetics Corporation | Blood-processing machine system |
| CN208931980U (en) * | 2018-08-16 | 2019-06-04 | 焦凤琴 | A kind of blood station blood storage device |
| CN212320171U (en) * | 2020-04-03 | 2021-01-08 | 孙运高 | Animal doctor preserves case with blood sample |
| CN213058147U (en) * | 2020-08-12 | 2021-04-27 | 浦城县明圣生物制品有限公司 | Animal blood holds and uses hierarchical storage device |
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