CN113480660B

CN113480660B - Chimeric terpene synthase and application thereof

Info

Publication number: CN113480660B
Application number: CN202110618456.4A
Authority: CN
Inventors: 刘天罡; 陈蓉; 卞光凯; 叶紫玲; 慕昕
Original assignee: Wuhan University WHU
Current assignee: Wuhan University WHU
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2022-11-18
Anticipated expiration: 2041-06-03
Also published as: CN113480660A

Abstract

The present invention provides a chimeric terpene synthase having terpene synthase and prenyltransferase activity. The chimeric terpene synthase has an amino acid sequence shown in any one of SEQ ID NO 1-11. The chimeric terpene synthase can catalyze a substrate to produce various terpenoids after fermentation culture. The invention provides a preparation method of several terpenoids, which is simple to prepare.

Description

Chimeric terpene synthase and application thereof

Technical Field

The invention relates to the technical field of biology, in particular to a chimeric terpene synthase and application thereof.

Background

Terpenoids refer to compounds having (C) ₅ H ₈ ) _n The general formula and derivatives thereof containing oxygen and having different saturation degrees can be regarded as a natural compound formed by connecting isoprene or isopentane in various ways. To date, about 76000 terpenoids have been found in animals, plants, and microorganisms. Terpenoids are widely used in the perfume industry, health care product industry, agricultural production field and medical industry. The application value of the terpenoid is very wide, and the discovery of more terpenoids has great practical significance.

In general, terpene Synthases (TS) synthesize the corresponding monoterpene, sesquiterpene, diterpene, sesterterpene and triterpenoid products using geranyl pyrophosphate (GPP), farnesyl diphosphate (FPP), geranylgeranyl diphosphate (GGPP) and geranylfarnesyl farnesyl diphosphate (GFPP) as substrates, whereas these different-chain-length isoprene pyrophosphate substrates are synthesized by the corresponding Prenyltransferases (PT) using isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), i.e. synthesis of the isopentenyl substrates and production of the terpenoids are accomplished by two enzymes independently catalyzing them, but PT also contains several chimeric domains for the same time, PT is found to be useful for the discovery of new chimeric structures for both the synthases.

Therefore, there is a need to develop new chimeric terpene synthases.

Disclosure of Invention

The present invention is directed to solving one of the problems of the background art, and thus, in a first aspect of the present invention, the present invention provides a chimeric terpene synthase having terpene synthase and prenyltransferase activities.

In one or more embodiments of the invention, the chimeric terpene synthase has an amino acid sequence set forth in any one of SEQ ID NOs 1-11.

In a second aspect of the invention, there is provided a nucleic acid molecule encoding the chimeric terpene synthase of the first aspect of the invention, the nucleic acid molecule having the nucleotide sequence set forth in any one of SEQ ID NOs 14 to 24.

In a third aspect, the invention provides a construct comprising a nucleic acid molecule according to the second aspect of the invention.

In a fourth aspect of the invention, the invention provides a recombinant cell comprising a nucleic acid molecule according to the second aspect of the invention.

In a fifth aspect, the invention provides a use of a chimeric terpene synthase according to the first aspect of the invention and/or a nucleic acid molecule according to the second aspect of the invention and/or a construct according to the third aspect of the invention and/or a recombinant cell according to the fourth aspect of the invention in the preparation of a terpenoid.

In one or more embodiments of the present invention, the terpenoid comprises at least one of the following structures,

in a sixth aspect of the present invention, the present invention provides a method for preparing a terpenoid, comprising: culturing the recombinant cell to obtain a culture product; and isolating the terpenoid from the culture product, the terpenoid comprising at least one of the following structures,

in a seventh aspect of the present invention, the present invention provides a terpenoid, which has a structure represented by structural formula 1 or structural formula 2,

compared with the prior art, the invention has the following advantages and beneficial effects:

1. the invention provides a chimeric terpene synthase which can catalyze a substrate to produce various terpene compounds after fermentation culture.

2. The invention provides a preparation method of several terpenoids, which is simple to prepare.

Drawings

FIG. 1 is a schematic diagram of the construction process of plasmid pZY 141;

FIG. 2 shows the GCMS detection spectrum of an organic phase after fermentation culture and treatment of a mutant strain containing PTTS 010;

FIG. 3 shows GCMS detection patterns of organic phase after fermentation culture and treatment of mutant strains containing PTTS009, PTTS037 and PTTS 054;

FIG. 4 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 008;

FIG. 5 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 021;

FIG. 6 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 052;

FIG. 7 shows the GCMS detection spectrum of an organic phase after the mutant strain containing PTTS119 is fermented and cultured;

FIG. 8 shows GCMS detection patterns of organic phase after fermentation culture and treatment of mutant containing PTTS 023;

FIG. 9 shows GCMS detection patterns of organic phase after fermentation culture of PTTS 013-containing mutants;

FIG. 10 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant strain containing PTTS 066;

FIG. 11 is a schematic representation of plasmid pZY 141;

in the figure, sesterevilene (1) represents compound 1, sesterorbicule (2) represents compound 2, β -gerylfarnesene (5) represents compound 5, gerylfarnesol (6) represents compound 6, geryllinaloool (23) represents compound 23, and gerylgeraniol (24) represents compound 24.

Detailed Description

The scheme of the invention will be explained with reference to the examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications. The methods used are conventional methods known in the art unless otherwise specified, and the consumables and reagents used are commercially available unless otherwise specified. Unless otherwise defined, technical and scientific terms used herein have the same meaning as is familiar to those skilled in the art. In addition, any methods or materials similar or equivalent to those described herein can also be used in the present invention.

Example 1 construction of vectors for Gene-digging Chassis Yeast strains

(1) Plasmid pZY141

△Gal1,7,10：：Trp_TACT1_tHMG1_pGAL10

The schematic construction process of plasmid pZY141 is shown in FIG. 1, and the specific process is as follows: using 30000B as a template, amplifying by using primers 1411-F/R to obtain a fragment 1141 (

Gal

1,7,10 left homology arms), amplifying by using primers 1415-F/R to obtain a fragment 1415 (bidirectional promoter Pgal10-pGAL 1), amplifying by using primers 1416-F/R to obtain a fragment 1416 (terminator tCPS 1), amplifying by using primers 1417-F/R to obtain a fragment 1417 (Gal, 7,10 right homology arms), amplifying by using pRS424 as a template by using primers 1412-F/R to obtain a fragment 1412 (tryptophan screening marker), amplifying by using S288C as a template by using primers 1413-F/R to obtain a fragment 1413 (terminator tACT 1), amplifying by using primers 1414-F/R to obtain a fragment 1414 (tHMG 1), amplifying by using pRS426 as a template by using primers 1418-F/R to obtain a fragment 1418 (vector backbone). These fragments were recombined by yeast assembly to give vector pZY141. Obtaining a fragment with a target gene after enzyme digestion linearization, wherein the nucleotide sequence is shown as SEQ ID NO: shown at 12. The primers used in the construction of plasmid pZY141 are shown in Table 1 below:

TABLE 1 primers used in the construction of plasmid pZY141

Example 2 construction of Gene-digging Chassis Yeast strains

The linearized fragment of plasmid pZY141 (nucleotide sequence shown in SEQ ID NO: 12) was transferred into the s.cerevisiae strain CEN. PK2-1D and integrated into the chromosome according to homologous recombination at the Gal1,7,10 site and the selection marker tryptophan (Trp 1), so that the background strain YZL141 was successfully constructed.

EXAMPLE 3 construction of a potential chimeric terpene synthase expression plasmid

Searching NCBI and Uniprot databases, limiting the amino acid length to 700-900, downloading all matched nucleotide/protein sequences, analyzing the downloaded sequences, screening sequences with terpene synthase conserved domains DDXXD/E and NSE/DTE, and carrying out PTTS gene synthesis to obtain the following PTTS genes: pUC57-008 (having a nucleotide sequence shown by SEQ ID NO: 14), pUC57-009 (having a nucleotide sequence shown by SEQ ID NO: 15), pUC57-010 (having a nucleotide sequence shown by SEQ ID NO: 16), pUC57-013 (having a nucleotide sequence shown by SEQ ID NO: 17), pUC57-021 (having a nucleotide sequence shown by SEQ ID NO: 18), pUC57-023 (having a nucleotide sequence shown by SEQ ID NO: 19), pUC57-037 (having a nucleotide sequence shown by SEQ ID NO: 20), pUC57-051 (having a nucleotide sequence shown by SEQ ID NO: 21), pUC57-054 (having a nucleotide sequence shown by SEQ ID NO: 22), pUC57-066 (having a nucleotide sequence shown by SEQ ID NO: 23), and pUC57-119 (having a nucleotide sequence shown by SEQ ID NO: 24). The PTTS gene was amplified using a plasmid synthesized by GeneCo as a template, and the plasmid backbone (nucleotide sequence shown in SEQ ID NO: 13) was amplified using pGB315 as a template. Then, the PTTS gene and the plasmid skeleton amplified by pGB315 are assembled by yeast assembly or Gibson respectively. In addition, in the construction process of the plasmid, the connecting peptide GSTGS reported previously is used for connecting GFPP synthetase and PTTSs, so that a fusion protein is formed, the physical and spatial distance between the PTTSs and GFPPS is shortened, and the metabolic flux of GFPP is improved to promote the functional characterization of sesterterpene synthase. In addition, the introduction of GFPPS can help release the utilization capacity of GFPP by the chimeric diterpene synthase, so that the chimeric diterpene synthase can generate sesterterpene compounds with various structures.

(1) Construction of plasmids the following Table

TABLE 2 plasmid Table

Serial number	Plasmids	Plasmid information
				1	pRC317	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS008-T _ADH1
2	pRC318	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS009-T _ADH1
			3	pRC319	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS010-T _ADH1
4	pRC320	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS013-T _ADH1
			5	pRC324	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS021-T _ADH1
6	pRC325	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS023-T _ADH1
			7	pRC333	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS037-T _ADH1
8	pRC345	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS051-T _ADH1
			9	pRC347	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS054-T _ADH1
10	pRC354	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS066-T _ADH1
			11	pRC377	pGB315 derived,URA,T _CYC1 -FDS-P _GAL1 -P _GAL10 -PTTS119-T _ADH1

(1) Plasmid pRC317 was constructed specifically: and (3) amplifying the gene pUC57-008 by using a primer PTTS008-F/R to obtain PTTS008, and amplifying the Vector skeleton by using a primer PTTS008V-F/R to obtain PTTS008-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC317. Plasmids pRC318, pRC319, pRC320, pRC324, pRC325, pRC333, pRC345, pRC347, pRC354 and pRC377 were obtained by the same construction. (2) Plasmid pRC318 specific construction Process: PTTS009 was obtained by amplifying pUC57-009 with PTTS009-F/R as a primer, and PTTS009-Vector was obtained by amplifying Vector backbone with PTTS009V-F/R as a primer. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC318. (3) The specific construction process of plasmid pRC 319: the gene pUC57-010 is amplified by the primer PTTS010-F/R to obtain PTTS010, and the Vector skeleton is amplified by the primer PTTS010V-F/R to obtain PTTS010-Vector. The obtained fragments were recombined by Gibson or yeast assembly to obtain plasmid pRC319. (4) Plasmid pRC320 was constructed specifically: the gene pUC57-013 was amplified with the primer PTTS013-F/R to obtain PTTS013, and the Vector backbone was amplified with the primer PTTS013V-F/R to obtain PTTS013-Vector. The obtained fragments were recombined by Gibson or yeast assembly to obtain plasmid pRC320. (5) The specific construction process of plasmid pRC 324: and (3) amplifying the gene pUC57-021 by using the primer PTTS021-F/R to obtain PTTS021, and amplifying the Vector skeleton by using the primer PTTS021V-F/R to obtain PTTS021-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC324. (6) Plasmid pRC325 specific construction Process: the gene pUC57-023 is amplified by the primer PTTS023-F/R to obtain PTTS023, and the Vector skeleton is amplified by the primer PTTS023V-F/R to obtain PTTS023-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC325. (7) The specific construction process of plasmid pRC 333: the gene pUC57-037 is amplified by the primer PTTS037-F/R to obtain PTTS037, and the Vector skeleton is amplified by the primer PTTS037V-F/R to obtain PTTS037-Vector. The obtained fragments are recombined in a Gibson or yeast assembly mode to obtain a plasmid pRC333. (7) Plasmid pRC325 specific construction Process: the gene pUC57-023 is amplified by the primer PTTS023-F/R to obtain PTTS023, and the Vector skeleton is amplified by the primer PTTS023V-F/R to obtain PTTS023-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC325. (8) Plasmid pRC345 specific construction Process: and amplifying the gene pUC57-051 by using the primer PTTS051-F/R to obtain PTTS051, and amplifying the Vector framework by using the primer PTTS051V-F/R to obtain PTTS051-Vector. The obtained fragments are recombined in a Gibson or yeast assembly mode to obtain a plasmid pRC345. (9) Plasmid pRC347 specific construction Process: the gene pUC57-054 is amplified by the primer PTTS054-F/R to obtain PTTS054, and the Vector skeleton is amplified by the primer PTTS054V-F/R to obtain PTTS054-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC347. Plasmid pRC354 specific construction procedure: the gene pUC57-066 is amplified by the primer PTTS066-F/R to obtain PTTS066, and the Vector skeleton is amplified by the primer PTTS066V-F/R to obtain PTTS066-Vector. The obtained fragments were recombined by Gibson or yeast assembly to obtain plasmid pRC 354. 11. Plasmid pRC377 concrete construction Process: the gene pUC57-119 was amplified with the primer PTTS119-F/R to obtain PTTS119, and the Vector backbone was amplified with the primer PTTS119V-F/R to obtain PTTS119-Vector. The obtained fragments were recombined by means of Gibson or yeast assembly to obtain plasmid pRC377.

TABLE 3 primer sequence Listing

Example 4 construction of potential chimeric terpene synthase expressing strains

Plasmids pRC317, pRC318, pRC319, pRC320, pRC324, pRC325, pRC333, pRC345, pRC347, pRC354 and pRC377 obtained in example 3 were transferred to the background strain YZL141 obtained in example 2, respectively, and the selection marker was uracil (URA 3), and the selection plate was Sc-URA (synthetic yeast nitrogen source YNB 6.7g/L, glucose 20g/L, uracil-deficient mixed amino acid powder 1.3g/L,2% agar powder), to obtain corresponding mutants.

The amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC317 is shown as SEQ ID NO:1 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC318 is shown in SEQ ID NO:2 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC319 is shown as SEQ ID NO:3 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC320 is shown in SEQ ID NO:4 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC324 is shown as SEQ ID NO:5 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC325 is shown as SEQ ID NO:6 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC333 is shown in SEQ ID NO:7 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC345 is shown as SEQ ID NO:8 is shown in the specification; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC347 is shown as SEQ ID NO:9 is shown in the figure; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC354 is shown as SEQ ID NO:10 is shown in the figure; the amino acid sequence of the chimeric terpene synthase expressed by the mutant strain corresponding to the plasmid pRC377 is shown as SEQ ID NO: shown at 11.

Example 5 Shake flask fermentation and product detection of potential chimeric terpene synthase expressing strains

The mutant strains obtained in example 4 were cultured by shake flask fermentation respectively, and the specific method was as follows: single clones were picked from the transformation plates into PA flasks containing 5mL of seed medium, the seed medium formulation was: YNB (6.7 g/L), yeast powder (10 g/L), glucose (20 g/L), uracil-deficient mixed amino acid powder (1.3 g/L). Carrying out transfer after shaking culture of seeds at 30 ℃ overnight, carrying out transfer according to the initial OD =0.1, placing the seeds on a shaking table at 30 ℃ to start shaking flask fermentation, wherein the formula of a fermentation medium is as follows: peptone (20 g/L), yeast powder (10 g/L), glucose (10 g/L) and galactose (10 g/L). And (3) collecting samples after fermentation is finished, and adding normal hexane: ethyl acetate (4. And finally, re-dissolving by using a small amount of normal hexane, and detecting the composition of the product by using organic phase GCMS. Semi-preparative purification and nuclear magnetic identification were performed on

compounds

1, 2, 5, 6, 23, 24.

The GC-MS detection results are shown in figures 2-10, wherein figure 2 shows the GCMS detection map of the organic phase after the PTTS 010-containing mutant strain is fermented and cultured and treated; the time to peak of compound 1 was 20.3min; FIG. 3 shows GCMS detection patterns of organic phase after fermentation culture and treatment of mutant strains containing PTTS009, PTTS037 and PTTS 054; the time to peak of compound 2 was 20.2; FIG. 4 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 008; the peak-off time of compound 5 was 22.0min; FIG. 5 shows GCMS detection spectra of treated mutant strains containing PTTS021 after fermentation culture; the time to peak of compound 6 was 21.9min; FIG. 6 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 052; the time to peak of compound 6 was 21.9min; FIG. 7 shows the GCMS detection spectrum of an organic phase after the mutant strain containing PTTS119 is fermented and cultured; the peak-off time of compound 6 was 21.9min; FIG. 8 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 023; the time to peak for compound 24 was 18.1min; FIG. 9 shows GCMS detection patterns of organic phase after fermentation culture of PTTS 013-containing mutants; the time to peak of compound 6 was 15.8min; the time to peak of compound 5 was 21.8min; FIG. 10 shows GCMS detection spectra of organic phase after fermentation culture and treatment of mutant containing PTTS 066; the time to peak of compound 24 was 28.1min and the time to peak of compound 6 was 21.9mi.

From the results of the experiments, it was found that the mutant strain containing PTTS010 can synthesize sestereviscene (Compound 1), which is a novel structural compound. PTTS009/PTTS037/PTTS054 can synthesize a sesterorbicule (Compound 2), a sesterorbicule compound, which is also a new structural compound. PTTS008 enables the synthesis of the beta-geranylfarnesene (compound 5), a sesterterpene compound. PTTS021/PTTS052/PTTS119 can synthesize the diterpene geranylfanesol compound (compound 6). PTTS023 can synthesize the diterpene compound geranylgeraniol (compound 24). PTTS013 can synthesize the diterpene compound geranyllinalol (compound 23) and the sesterterpene compound beta-geranylfarnesene (compound 5). PTTS066 allows the synthesis of the diterpenoid compound geranylgeraniol (compound 24) and the sesterterpene compound geranylfarnesol (compound 6).

Thus, the isopentenyl transferase domain of the chimeric terpene synthase provided by the invention can synthesize geranylgeranyl pyrophosphate (GGPP) or geranylfarnesyl diphosphate (GFPP) by taking isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) as substrates, and synthesize different types of diterpenes and sesterterpene products catalyzed by the terpene synthase domain.

Hydrogen spectra, carbon spectra, and oxygen spectra of the

compounds

1, 2, 5, 6, 23, and 24, ¹ H- ¹ The H COSY and HMBC spectral data are shown below.

The following Table 4 shows the hydrogen spectrum, carbon spectrum, and, ¹ H- ¹ H COSY and HMBC spectrogram data

TABLE 4 hydrogen spectrum, carbon spectrum, of Compound 1, ¹ H- ¹ H COSY and HMBC spectrogram data

Table 5 shows the hydrogen spectrum, carbon spectrum, and, ¹ H- ¹ H COSY and HMBC spectrogram data

TABLE 5 hydrogen spectrum, carbon spectrum, of Compound 2, ¹ H- ¹ H COSY and HMBC spectrogram data

The following table 6 shows hydrogen spectra of compound 5 and compound 6: ( ¹ H NMR，CDCl ₃ 500 MHz) and carbon spectrum ( ¹³ C NMR，CDCl ₃ 125 MHz) data

TABLE 6 Hydrogen spectra of Compound 5 and Compound 6: ( ¹ H NMR，CDCl ₃ 500 MHz) and carbon spectrum ( ¹³ C NMR，CDCl ₃ 125 MHz) data

The following table 7 is a hydrogen spectrum of compound 23 and compound 24 (see ¹ H NMR，CDCl ₃ 500 MHz) and carbon spectrum ( ¹³ C NMR，CDCl ₃ 125 MHz) data

TABLE 7 Hydrogen spectra of Compound 23 and Compound 24: ( ¹ H NMR，CDCl ₃ 500 MHz) and carbon spectrum ( ¹³ C NMR，CDCl ₃ 125 MHz) data

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Sequence listing

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Gly Leu Asp Ile Tyr Trp Gly Lys Thr Val Phe Gly Leu Gly Glu Lys

195 200 205

Leu Thr Asp Asp Glu Glu Lys Leu Ile Arg Pro Leu Val Trp Ala Ala

210 215 220

Glu Lys Ala Ala Met Leu Asn Asn Asp Tyr Trp Ser Trp Asp Val Glu

225 230 235 240

Tyr Ser Glu Ala Asn Gln Lys Thr Glu His Leu Thr Asn Ala Val Ala

245 250 255

Val Leu Met Lys Asn Glu Gly Ile Ser Ala Glu Glu Gly Lys Glu Arg

260 265 270

Ile Lys Gly Leu Ile Ile Asp Tyr Glu Ala Glu Tyr Leu Arg Leu Arg

275 280 285

Ala Gln Phe Tyr Glu Asn His Pro Ser Ala Arg Leu Tyr Leu Arg Lys

290 295 300

Arg Val Glu Leu Ala Gly Ser Met Ala Ala Gly Val Ser Phe Trp Ser

305 310 315 320

Ala Asn Ser Pro Arg Tyr His Leu Ser Lys Lys Gln Val Glu Ala Ala

325 330 335

Gly Leu Pro Asp Asp Pro Arg Thr Lys Pro Asn Gln Thr Ser Asp Thr

340 345 350

Gly Ser Pro Met Gly Arg Ser Ser Thr Asp Ser Gln Val Ala Ser Asp

355 360 365

Ile Thr Trp Ser Thr Ser Thr Asp Ile Ser Ser Gln Ser Gln His Thr

370 375 380

Gln Asp Leu Val Ala His Ser Tyr Tyr Gly Val Pro Lys Leu Asp Arg

385 390 395 400

Thr Ala Leu Asn Gly Pro Ile Glu Tyr Val Ser Ala Met Pro Ser Lys

405 410 415

Gly Val Arg Ser Ser Leu Ile Asp Ala Met Asn Gln Trp Cys Gln Val

420 425 430

Pro Ser Ser Gln Leu Ala Val Val Lys Ser Val Ile Asp Gln Leu His

435 440 445

Asn Ser Ser Leu Val Leu Asp Asp Ile Gln Asp Asp Ser Pro Met Arg

450 455 460

Arg Gly Lys Thr Ala Thr His Leu Ile Phe Gly Gln Ala Gln Ala Ile

465 470 475 480

Asn Ser Ala Asn Phe Leu Tyr Val Arg Val Val Gln Glu Val His Ala

485 490 495

Thr Arg Asn Thr Ala Leu Met Asp Ile Leu Leu Glu Glu Leu Glu Asp

500 505 510

Leu His Val Gly Gln Ser Trp Asp Leu Tyr Trp Lys Tyr Asn Leu Arg

515 520 525

Trp Pro Ser Glu Ala Glu Tyr Phe Ser Met Ile Asp Leu Lys Thr Gly

530 535 540

Gly Leu Phe Arg Met Leu Val Arg Met Met Gln Thr Leu Ser Pro Leu

545 550 555 560

Lys Asp Arg Asp Phe Val Cys Asp Thr Leu Val Ser Met Val Ser Arg

565 570 575

Phe Phe Gln Val Arg Asp Asp Tyr Leu Asn Leu Asn Ser Arg Glu Tyr

580 585 590

Ser Thr Gln Lys Gly Trp Cys Glu Asp Leu Asp Glu Gly Lys Phe Ser

595 600 605

Tyr Leu Ile Ile His Cys Leu Gln His Ser Pro Lys Phe Arg Asp Arg

610 615 620

Ile Met Gly Phe Phe Arg Gln Arg Thr Gly Cys Val Gly Pro Met Pro

625 630 635 640

Thr Val Gly Lys Val Gln Ile Ile Glu Tyr Leu Gln Glu Ala Gly Ser

645 650 655

Phe Asn Ala Cys Trp Glu Leu Leu Asn Ser Leu Glu Asp Asp Ile Glu

660 665 670

Lys Glu Ile Lys Arg Leu Glu Glu Ile Thr Gly Glu Lys Asn Pro Leu

675 680 685

Met His Leu Leu Leu Lys Leu Leu Ser Val Lys Thr Glu Lys Pro Asn

690 695 700

Gly Lys Ala Val Val Ala Pro Ala Gly Leu

705 710

<210> 3

<211> 728

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 3

Met Ala Glu Phe Ala Ile Pro Val Pro Asp Asp Val Val Lys Gln Ser

1 5 10 15

Gly Thr Leu Ser Arg Phe Pro Thr Ala Val His Arg Glu His Ala Arg

20 25 30

Cys Leu Ala Ala Ala Asn Lys Ile Arg Asp Asp Phe Ala Ala Gln Val

35 40 45

Asp Trp Asp Leu Asp Ala Lys Thr Thr Gly His Tyr Pro Thr Leu Gly

50 55 60

Ala Val His Val Val Ala Phe Thr Met Pro Glu Cys Leu Pro Glu Arg

65 70 75 80

Leu Ala Leu Met Thr Arg Phe Thr Asp Phe Thr Ile Met Asn Asp Asp

85 90 95

Tyr Tyr Asp Ala Val Asp Arg Asp Gln Ala Thr Ser Phe Asn Ala Glu

100 105 110

Leu Gln Arg Ser Leu Gly Arg Asp Cys His Ser Asn Thr Val Gln Gly

115 120 125

Asn Ala Ser Val Ala Ile Lys Thr Lys Gln Phe Gln Ala Ser Ile Leu

130 135 140

Val Glu Met Met Val Met Asp Arg Asp Leu Ala Met Asp Val Met Asp

145 150 155 160

Thr Tyr Ser Asp Gly Leu Glu Thr Ala Thr Phe Pro Pro Ser Asp Ile

165 170 175

Cys Thr Ile Glu Glu Tyr Leu Pro Val Arg Leu Val Asn Cys Gly Leu

180 185 190

Asp Val Phe Gln Glu Met Ser Cys Phe Gly Leu Gly Val His Leu Thr

195 200 205

Lys Ala Glu Lys Glu Lys Leu Ser Asp Ile Ala Asn Thr Ala Leu Tyr

210 215 220

Thr Ala Ala Leu Ile Asn Asp Cys His Ser Trp Pro Lys Glu Leu Lys

225 230 235 240

His His Leu Glu Thr Pro Gly Ser Asp Val Pro Phe Asn Ala Val Cys

245 250 255

Ile Leu Met Arg Gln Phe Asn Cys Ser Asp Val Lys Ala Ile Glu Arg

260 265 270

Leu Arg Ala Ile Tyr Val Glu Ile Gln Glu Arg His Leu Ser Leu Val

275 280 285

Arg Asn Leu Glu Gln Ser Glu Gly Ser Ile Pro Glu Thr His Arg Lys

290 295 300

Tyr Ile Met Ala Ala Gln Tyr Ala Ala Ser Gly Ser Glu Phe Trp Ser

305 310 315 320

Leu Tyr Ala Pro Arg Tyr Pro Ser Lys Glu Asp Leu Glu Gln Pro Glu

325 330 335

Tyr Val Leu Val Asp Asn Val Leu His Arg Arg Ser Met Ser Asp Lys

340 345 350

Asp Leu Pro Thr Ser Asp Lys Asp Leu Ala Arg Ala Asp Ser Ala Met

355 360 365

His Ile Glu Thr Ile Lys Thr Ala Gly Ser Ser Gly Met Ser His Met

370 375 380

Asn Glu Ala Tyr Ser Ser Thr Pro Ala Thr Glu Met Val Ala Trp Asp

385 390 395 400

Ala Gly Ser Glu Ile Ile His Thr Glu Ile Asp Ser Asn Gly Ser Lys

405 410 415

Glu Leu Ala Pro Asn Gly Ala Gln Thr Arg Val Gln Lys Pro Ser Glu

420 425 430

Asp Ala Val Arg Ala Pro Tyr Asp Tyr Ile Arg Ala Leu Pro Ser Lys

435 440 445

Arg Ile Arg Glu Thr Phe Ile Asp Ala Leu Asp Ser Trp Leu Ala Val

450 455 460

Pro Ala Gly Ser Ser Ala Ser Ile Lys Ser Ile Ile Gly Met Leu His

465 470 475 480

Gln Ser Ser Leu Met Leu Asp Asp Ile Glu Asp Asp Ser Thr Leu Arg

485 490 495

Arg Gly Lys Pro Thr Ala His Thr Leu Phe Gly Ile Ala Gln Thr Ile

500 505 510

Asn Ser Ala Asn Trp Val Phe Ala Cys Ala Phe Glu Glu Leu Arg Ser

515 520 525

Leu Arg Gly Val Asp Ala Ala Thr Ile Phe Val Glu Glu Val Lys Asn

530 535 540

Leu His Cys Gly Gln Ala Leu Asp Leu His Trp Lys His His Thr Tyr

545 550 555 560

Ile Pro Ser Val Asp Glu Tyr Leu Asn Met Val Asp His Lys Thr Gly

565 570 575

Gly Leu Phe Arg Leu Cys Val Arg Leu Met Gln Gly Glu Ser Ser Thr

580 585 590

Ser Cys His His Ile Asp Ala Glu Arg Phe Ile Thr Leu Leu Gly Arg

595 600 605

Tyr Phe Gln Ile Arg Asp Asp Tyr Gln Asn Leu Val Ser Asp Glu Tyr

610 615 620

Thr Asn Gln Lys Gly Phe Cys Glu Asp Leu Asp Glu Gly Lys Ile Ser

625 630 635 640

Leu Pro Leu Ile Tyr Cys Leu Ala Gly Ser Asp Pro Thr Gln Ile Met

645 650 655

Ile Arg Gly Ile Leu Gln His Lys Arg Ala Gly Glu Met Pro Leu Ser

660 665 670

Met Lys Lys Leu Ile Leu Glu Lys Met Arg Ser Gly Gly Ala Leu Asn

675 680 685

Ala Thr Ile Ser Leu Leu Lys Asp Leu Gln Asp Asn Ile Leu Glu Glu

690 695 700

Leu Lys Ser Leu Glu Ser Ala Phe Gly Ser Gly Asn Pro Met Leu Glu

705 710 715 720

Leu Val Leu Arg Arg Leu Trp Ile

725

<210> 4

<211> 684

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 4

Met Ser Pro Val Asp Ile Ser Phe Ile His Ser Glu Leu Val Asp Arg

1 5 10 15

Glu Glu Val Ala Arg Val Cys Ala Thr Thr Leu Pro Val Arg Lys Ser

20 25 30

Lys Tyr Ser Pro Leu Val Glu Lys Ala Val Leu Glu Phe Gln Gln Gln

35 40 45

Trp Gln Glu Glu Val Gly Phe Ala Tyr Cys Gly Ala Thr Ser Pro Gln

50 55 60

Gly Pro Val Thr Val Phe Phe Pro Pro Glu Thr Lys Gln Asp Arg Val

65 70 75 80

Glu Ile Phe Ala Lys Leu Ile Glu Tyr Phe Phe Ala Tyr Asp Asp Val

85 90 95

Leu Thr Ala Pro Gly Ser Ala Lys Thr Gly Glu Glu Gln Ser Ser Asp

100 105 110

Ser Ile Gly Trp Glu Val Arg Lys Gly Thr Thr Ser Arg Val Arg Ile

115 120 125

Ser Ala Met Lys Gln Ile Gln Ser Glu Val Phe Leu Arg Leu Leu Glu

130 135 140

Ile Asp Arg Lys Arg Gly Asn Leu Ile Leu Arg Ala Ile Asn Asp Leu

145 150 155 160

Ser Arg Val His Gly Thr Ile Gly Ser Arg Asp Leu Arg Thr Trp Asp

165 170 175

Asp Leu Val Gln Tyr Arg Ala Asp Asp Tyr Gly Ala Glu Leu Asn Ile

180 185 190

Met Ser Ile Ile Tyr Cys Cys Glu Leu Asp Leu Thr Gln Gly Asp Ile

195 200 205

Gln Ala Leu Lys Glu Ile Trp Ser Pro Ala Thr Ala Ala Ala Ala Leu

210 215 220

Val Asn Asp Leu Tyr Ser Phe Asn Arg Glu Val Ile Leu Glu Pro Asp

225 230 235 240

Thr Asp Thr Asp Thr Thr Ile Thr Thr Pro Asn Ser Val Trp Tyr Leu

245 250 255

Met Lys Thr Leu Asn Leu Ser Val Ser Gln Ala Lys Glu Phe Leu Val

260 265 270

Lys Asp Lys Ile Leu Pro Leu Glu Arg Glu Phe Ile Ala Lys Lys Ala

275 280 285

Glu Tyr Leu Ala Asn Met Asn Pro Leu Thr Pro Lys Ser Gly Asp Ile

290 295 300

Ile Tyr Phe Leu Glu Met Val Gly Leu Gly Leu Ser Gly Asn Trp Tyr

305 310 315 320

Trp His Ala Ile Ala Asp Arg Phe His Arg Trp Ala Glu Leu Leu Gln

325 330 335

Leu Pro Pro Ala Lys Leu Phe Asp Tyr Asp Glu Ala Thr Ala Thr Cys

340 345 350

Ala Thr Phe Leu Asn Thr Gln Ser Leu Arg Gly Arg Lys Asn Arg Ile

355 360 365

Leu Glu Ser Thr Glu Ser Leu Gln Ala Met Thr Asp Asp Pro Tyr Tyr

370 375 380

Lys Val Leu His Gln Pro Ile Asp Tyr Leu Arg Ser Val Pro Ser Lys

385 390 395 400

Asn Ile Arg Gly Thr Ile Ile Gln Ala Leu Asn Leu Trp Leu Asn Ala

405 410 415

Pro Glu Ser Ala Ala Ala Gln Val Glu Asp Leu Ile Gly His Leu His

420 425 430

Glu Ser Ser Leu Leu Leu Asp Asp Ile Gln Asp Ser Ser Glu Leu Arg

435 440 445

Arg Gly Arg Pro Ser Ala Tyr Arg Val Phe Gly Val Pro Gln Thr Ile

450 455 460

Asn Ala Ala Thr His Ala Leu Thr Leu Ala Phe Glu Lys Val Val Pro

465 470 475 480

Leu Met Lys Pro Asp Ser Ser His Val Phe Phe Asp Glu Leu Arg Asn

485 490 495

Leu His Val Gly Gln Ala Met Asp Leu Tyr Trp Thr Arg Ser Gly Tyr

500 505 510

Arg Pro Ser Ile Ala Glu Tyr Leu Glu Met Asn Arg Leu Lys Thr Gly

515 520 525

Ala Leu Phe Cys Leu Ala Ser Asn Leu Leu Ser Asn Gln Gly Ser Phe

530 535 540

Ser Ala Gly Ala Ile Lys Gln Thr Asp Leu Asn Asp Leu Met Ile Ser

545 550 555 560

Leu Gly Gln Tyr Phe Gln Ala Arg Asp Asp Tyr Ile Asn Leu Ala Ser

565 570 575

Thr Lys Tyr Gln Glu Gln Lys Gly Phe Ala Gln Asp Leu Asp Glu Gly

580 585 590

Lys Leu Ser Leu Pro Leu Ile His Leu Leu Thr Gln Ser Pro Asn Ala

595 600 605

Ala Leu Ile Glu Asn Ile Gln Gln Glu Arg Ala Arg Asn Asn Lys Leu

610 615 620

Pro Ala Asp Leu Lys Gln Leu Ile Leu Asp Glu Met Arg Asp Gln Lys

625 630 635 640

Ile Met Gln Leu Thr Glu Glu Thr Leu Asn Gly Leu Glu Ala Lys Val

645 650 655

Tyr Arg Tyr Leu Glu Arg Leu Glu Val Ser Ala Gly Ile Lys Asn Phe

660 665 670

Thr Phe Arg Phe Leu Leu Asp Arg Leu Arg Glu Met

675 680

<210> 5

<211> 699

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 5

Met Asp Leu Pro Thr Ser Glu Leu Asp Ala Thr Trp Thr Asn Ser Lys

1 5 10 15

Ser Val Asp Leu Thr Arg Glu Arg Pro Pro Asp Cys Phe Thr Thr Arg

20 25 30

Ala Val Arg Ile Asn Lys Cys Asp Asp Val Met Asn Leu Tyr Thr Thr

35 40 45

Lys Ala Ile Asn Asn Trp Thr Ala Ala Met Cys Asp Asp Ala Asp Arg

50 55 60

Lys Val Gly His Ala Phe Ser Ser Val Gly Asn Val Ser Ser Phe Ile

65 70 75 80

Phe Pro Glu Cys Ser Pro Asp Lys Ala His Ile Leu Ala Tyr Phe Thr

85 90 95

Gln Val Ser Phe Ile His Asp Asp Ala Leu Ser Ser Lys Asp Ser Lys

100 105 110

Glu Glu Asn Lys His Leu Ser His Ala Leu Asp Pro Lys Asp Asn Asn

115 120 125

Pro Gly Thr Ser Glu Arg Gly Lys Ala Met Lys Lys Phe Leu Ser Gln

130 135 140

Thr Val Leu Glu Leu Ile Asp Met Asp Thr Asn Glu Gly Gln Glu Phe

145 150 155 160

Val Lys Asn Leu Lys Val Trp Ala Asp Asp Glu Ile Gly Leu Lys Asn

165 170 175

Pro Gln Thr Ile Glu Cys Val Asp Glu Tyr Leu His Phe Arg Arg Leu

180 185 190

Asn Gly Gly Ile Lys Ala Tyr Trp His Trp Leu Ala Phe Ser His Glu

195 200 205

Asp Arg Phe Thr Gln Ala Asp Trp Asp Ser Ile Glu Asp Leu Leu Lys

210 215 220

Ser Ala Asn Arg Val Phe Ile Leu Thr Asn Asp Tyr Phe Ser Trp Pro

225 230 235 240

Arg Glu Arg Leu Tyr Gly Gln Gly Arg Ile Ala Asn Val Ile Glu Phe

245 250 255

Tyr Met Arg Thr Glu Gly Leu Ser Glu Glu Glu Ala Lys Gln Arg Thr

260 265 270

Lys Glu Glu Ile Leu Gln Gly Glu His Leu Phe His Asn Met Cys Val

275 280 285

Glu Arg Phe Ala Arg Glu Pro Asn Leu Pro Arg His Val Lys Lys Leu

290 295 300

Leu Gln Val Ala Glu Val Ala Met Gly Gly Tyr Asn Tyr Trp Ala Ser

305 310 315 320

Thr Cys Pro Arg Leu Asn Ser Trp Lys Glu Gln Ala Pro Thr Ala Glu

325 330 335

Thr Asp Phe His Gly Ser Lys Asn Asp Glu Val Ser Asn Pro Thr Gly

340 345 350

Lys Ala Glu Leu Val Lys Pro Val Lys Thr Asn Gln Thr Ser Gly Lys

355 360 365

Ile Gln Ala Ile Thr Lys Val Gln Ser Leu Pro Ser Val Ser His Phe

370 375 380

Thr Cys Thr Ser Asp Leu Asp Asp Ser Ala Leu Leu Ala Pro Ala His

385 390 395 400

Tyr Val Glu Ser Leu Ser Ser Lys Asn Val Leu Ser Lys Leu Val Glu

405 410 415

Ala Phe Asn Val Trp Met Gln Val Pro Pro Lys Pro Leu Ala Ala Ile

420 425 430

Lys His Val Leu Asp Asp Leu His Asn Ser Ser Leu Ile Leu Asp Asp

435 440 445

Ile Gln Asp Asn Ser Pro Leu Arg Arg Gly Arg Thr Ala Thr His Leu

450 455 460

Ile Phe Gly Pro Ala Gln Ser Ile Asn Ser Ala Thr Tyr Met Phe Val

465 470 475 480

Lys Ala Ala Gln Thr Val Asp Ala Leu Gly Thr Pro Gln Met Met Thr

485 490 495

Ala Phe Leu Gln Gly Leu Glu Thr Leu Phe Ile Gly Gln Ser Trp Asp

500 505 510

Ile Ser Trp Arg Gln Ser Phe His Cys Pro Thr Glu Ser Glu Tyr Leu

515 520 525

Ser Ala Ala Asp Lys Lys Thr Gly Ala Leu Leu Thr Met Met Val Glu

530 535 540

Leu Met Gln Cys Asn Ala Lys Thr Leu Pro Phe Ser Tyr Arg Leu Ser

545 550 555 560

Pro Leu Ala Arg Leu Phe Gly Arg Trp Tyr Gln Val Arg Asp Asp Tyr

565 570 575

Met Asn Leu Gln Gly Ala Asp Tyr Ser Lys Gln Lys Gly Phe Cys Glu

580 585 590

Asp Leu Asp Glu Gly Lys Leu Ser Tyr Pro Ile Leu Lys Cys Cys Gln

595 600 605

Lys Ser Glu Thr Asn Lys Gly Ile Ile Leu Gly Ile Phe Arg Gln Met

610 615 620

Arg Met Thr Asn Thr Lys Met Met Arg Glu Ser Lys Leu His Ile Leu

625 630 635 640

Asp Leu Met Ser Ser Ala Arg Ala Leu Glu Asp Thr Phe Asp Tyr Leu

645 650 655

Gln Gln Leu Gln Gln Glu Ile Glu Arg Asp Ile Arg Glu Ile Glu Val

660 665 670

Leu Ala Gly Glu Ser Asn Pro Glu Leu Leu Leu Leu Val Lys Val Leu

675 680 685

Gly Ala Ile Pro Lys Pro Gly Lys Lys Gly His

690 695

<210> 6

<211> 746

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 6

Met Asp Met Leu Asp Gly His Thr Ser Glu Leu Ile Gly Leu Ser Arg

1 5 10 15

Tyr Asp Thr His Gly Phe Leu Ser Pro Gly Gly Tyr Thr Leu Arg Arg

20 25 30

His Lys Ala Glu Thr Leu Ala Asn Ala Gly Cys Tyr Glu Ala Arg Gln

35 40 45

Asp Trp Ile Gln Tyr Ile Gly Ala Ala Ala Glu Glu Phe Gly Asn Cys

50 55 60

Asn Pro Val Asn Gly Asn Phe Thr Ala Val Val Leu Pro Leu Ala Lys

65 70 75 80

Pro Glu Arg Leu Arg Leu Val Ala Tyr Ile Leu Glu Tyr Ala Phe Leu

85 90 95

His Asp Asn Ile Val Glu Val Ala Lys Asp Asn Ala Ser Ser Ser Lys

100 105 110

Asp Asn Asp Ala Phe Ser Leu Gly Asp Met Glu Thr Ser His Gln Asn

115 120 125

Ala Lys Leu Gly Arg Lys Gln Ile Gln Ala Lys Met Ile Leu Gln Leu

130 135 140

Thr Gln Thr Asp Pro Val Cys Ala Lys Arg Val Met Gly Val Trp Gln

145 150 155 160

Gln Met Leu Glu Thr Thr Ile Lys Asp Lys Ser Lys Ser Phe Ala Asn

165 170 175

Leu Glu Glu Tyr Leu Glu Phe Arg Ile Ile Asp Thr Gly Ala Pro Phe

180 185 190

Val Glu Ser Val Met Leu Phe Gly Leu Gly Glu Thr Leu Thr Lys Glu

195 200 205

Glu Asp Ser Leu Leu Ala Pro Ile Ile Arg Pro Cys Tyr Ala Ser Leu

210 215 220

Ala Leu Ala Asn Asp Tyr Phe Ser Phe His Arg Glu Trp Ala Glu Tyr

225 230 235 240

Gln Ser Ala Gln Ser Ser Ser Ser Ser Ser Pro Asp Ala Pro Ser Ala

245 250 255

Ala Pro Ala Ala Pro Ile Asn Leu Val Tyr Leu Tyr Thr Gln Trp Gln

260 265 270

Ser Val Asp Ile Pro Thr Ala Lys Arg Leu Val Arg Glu Ala Ala Asn

275 280 285

Arg Phe Glu Ala Asp Phe Leu Arg Lys Cys Glu Asp Phe Lys Lys Ser

290 295 300

Gly Gln Ser Asn Asp Lys Leu Asp Arg Tyr Leu Arg Gly Leu Gln Tyr

305 310 315 320

Gln Val Ser Gly Asn Val Val Trp Ser Leu Asn Cys Pro Arg Tyr His

325 330 335

Pro Glu Trp Arg Tyr Asp Pro Asn Lys Gly Leu Glu Asp Ala Leu Thr

340 345 350

Ala Glu Arg Arg Asn Pro Pro Phe Val Glu Gln Val Glu Asp Val Asp

355 360 365

Ala Ser Val Ser Asp Lys Glu Ala Ala Thr Lys Arg Met Ser Ile Ala

370 375 380

Ser Gly Gly Ser Arg Glu Asn Asp Ser Asp Val Ser Thr Thr Ser Ser

385 390 395 400

Ser Trp Asp Asp Arg Ala Ala Ser Arg Ser Ser Ser Phe Ser Ala Met

405 410 415

Leu Asp Asp Asp Glu Ser Gly Met Arg Glu Phe Lys Glu Ala Gln Arg

420 425 430

Glu Leu Ser Leu Asp Ser Leu Leu Pro Ser Glu Glu Lys Leu Gly Met

435 440 445

Glu Ile Val Asn Ala Pro Phe Glu Tyr Thr Arg Leu Met Pro Ser Lys

450 455 460

Asn Val Arg Gly Thr Phe Ile Asp Ala Leu Asn Leu Trp Ala Gly Leu

465 470 475 480

Ser Glu Glu Val Leu Gly Gln Ile Lys Glu Val Val Asp Asp Leu His

485 490 495

Thr Ala Ser Leu Met Phe Asp Asp Val Glu Asp Gly Ser Glu Leu Arg

500 505 510

Arg Gly Asn Pro Ala Ala His Ala Val Tyr Gly Ile Pro Gln Thr Ile

515 520 525

Asn Ser Ala Ser Leu Ala Ile Leu Glu Ala Val Gln Lys Ala Lys Asp

530 535 540

Leu Pro Ile Pro Gly Ala Val Asp Ile Ala Leu Glu Gln Leu Arg Asp

545 550 555 560

Leu His Val Gly Gln Ser Tyr Asp Leu Tyr Trp Thr Arg His Met Thr

565 570 575

Cys Pro Ser Glu Ser Glu Tyr Leu Glu Met Val Ala Lys Lys Thr Gly

580 585 590

Gly Leu Phe Leu Leu Leu Ser Arg Leu Met Ser Glu His Met Pro Lys

595 600 605

Glu Val Arg Ser Leu Val Asn Asp Leu Val Thr Gln Val Gly Ile Tyr

610 615 620

Phe Gln Ile Arg Asp Asp Tyr Gln Asn Leu Ser Ser Asp Glu Tyr Thr

625 630 635 640

Ala Gln Lys Gly Phe Cys Glu Asp Leu Asp Glu Gly Lys Leu Ser Phe

645 650 655

Pro Leu Val His Tyr Leu Asn Thr Glu Arg Gly Ser Ser Asn Ser Gln

660 665 670

Gln Val Arg Glu Val Leu Gln Glu Arg Gln Leu Arg Gly Ser Leu Ser

675 680 685

Met Pro Leu Lys Leu Leu Thr Leu Gln Arg Leu Lys Ser Ser Asn Ser

690 695 700

Leu Glu Tyr Thr Arg Asp Ser Leu Met Arg Leu Glu Arg Gly Val Asp

705 710 715 720

Gly Thr Ile Glu Glu Leu Glu Arg Ser Thr Gly Lys Lys Asn Trp Val

725 730 735

Leu Arg Met Cys Met Ala Lys Leu Ser Val

740 745

<210> 7

<211> 757

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 7

Met Ala Ser Glu Met Trp Lys Tyr Ser Ser Pro Ile Asp Pro Glu Val

1 5 10 15

Val Lys Ala Thr Gly Cys Phe Thr Thr Leu Pro Val Arg Ile Asn Asn

20 25 30

Arg Asp Asp Leu Ala Asn Gly Ala Ser Ser Arg Val Leu Lys Asp Trp

35 40 45

Ala Glu His Thr Gly Asn Gln Ser Ile Asp Arg Asn Arg Val Ser Phe

50 55 60

Ser Pro Val Gly Ser Phe Cys Ser Leu Ile Tyr Cys Glu Thr Ile Pro

65 70 75 80

Glu Arg Leu Asp Ser Ile Ser Tyr Leu Thr Asp Leu Phe Phe Leu Ile

85 90 95

Asp Asp Ala Thr Glu Glu Val Glu Asn Asp Thr Leu Ala Gln Glu Gln

100 105 110

Trp Ala Gly Phe Ser Gly Ala Met Thr Asp Ser Leu Ala Glu Ala Ala

115 120 125

Pro Gln Arg Asp His Asp Leu Asp Met Met Lys Lys Lys Lys Leu Val

130 135 140

Ala Arg Val Met Leu Asp Phe Met Arg Leu Asp Pro Val Leu Gly Leu

145 150 155 160

Asp Leu Val Lys Ser Cys Lys Ala Gly Trp Thr Pro Leu Thr Ala Gly

165 170 175

Val Glu Trp Glu Thr Met Glu Asp Tyr Leu Arg Phe Arg Arg Leu Ser

180 185 190

Ala Gly Leu Asp Ile Tyr Trp Thr Lys Thr Val Phe Gly Leu Gly Glu

195 200 205

Lys Leu Thr Asp Asp Glu Glu Lys Leu Ile Arg Pro Leu Thr Trp Ala

210 215 220

Ala Glu Lys Ala Ala Met Leu Asn Asn Asp Tyr Trp Ser Trp Asp Ile

225 230 235 240

Glu Tyr Leu Gln Ala Gly Gly Asn Ile Glu Lys Leu Thr Asn Ala Val

245 250 255

Ala Val Leu Met Arg Lys Glu Gly Leu Thr Ala Glu Glu Gly Lys Gln

260 265 270

Arg Ile Lys Asn Leu Ile Ile Gly Tyr Glu Glu Glu Tyr Ser Arg Leu

275 280 285

Arg Asp Asp Phe Tyr Asn Ala His Pro Ser Ala Arg Leu Tyr Leu Arg

290 295 300

Lys Arg Val Glu Leu Ala Gly Ser Met Ala Ala Gly Val Ser Phe Trp

305 310 315 320

Ser Ala Asn Ser Pro Arg Tyr His Ile Pro Thr Gln Gln Thr Glu Thr

325 330 335

Thr Thr Glu Ser Gly Glu Ala Ala Gln Pro Ala Ala Gly Thr Trp His

340 345 350

Ala Glu Val Arg Asp Thr Ser Arg Pro Gly Ser Asp Ser Ser Val Ser

355 360 365

Ser Thr Ser Glu Ser Asp Asp Ser Ile Asp Ala Ser Ala Ser Ser Gly

370 375 380

Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Ala Leu Ser Ser Ser Gln

385 390 395 400

Thr Ser Leu Ser Ser Ser Leu Ser Glu Ala Glu Glu Glu Glu Glu Ala

405 410 415

His Lys Pro Pro Pro Cys Phe Asp Ala Pro Ala Lys Leu Gly Arg Ala

420 425 430

Ala Ile Asp Ala Pro Ile Asp Tyr Val Ser Gly Met Pro Ser Lys Gly

435 440 445

Val Arg Thr Ser Leu Ile Asp Ala Met Asn Gln Trp Cys Arg Val Pro

450 455 460

Ser Ala Gln Leu Gly Ala Val Lys Arg Val Val Asp Val Leu His Asn

465 470 475 480

Ser Ser Leu Ile Leu Asp Asp Ile Gln Asp Asp Ser Pro Met Arg Arg

485 490 495

Gly Lys Thr Ala Thr His Leu Val Phe Gly Ala Ala Gln Ala Ile Asn

500 505 510

Ser Ala Thr Phe Leu His Val Arg Ala Val Arg Glu Val His Ala Thr

515 520 525

Gly Ser Ala Ala Leu Met Ala Val Leu Leu Glu Glu Leu Glu Asp Leu

530 535 540

His Val Gly Gln Ser Trp Asp Leu Tyr Trp Lys Tyr Asn Leu Arg Trp

545 550 555 560

Pro Thr Glu Asp Glu Tyr Phe Ser Met Ile Asp Leu Lys Thr Gly Gly

565 570 575

Leu Phe Arg Met Leu Val Arg Met Met Arg Val Leu Ala Pro Glu Pro

580 585 590

Thr Gly Gly Glu Thr Lys Gly Gly Glu Phe Ala Cys Asp Ala Leu Val

595 600 605

Ser Met Val Ser Arg Phe Phe Gln Val Arg Asp Asp Tyr Leu Asn Leu

610 615 620

Asn Ser Arg Glu Tyr Gly Ser Gln Lys Gly Trp Cys Glu Asp Leu Asp

625 630 635 640

Glu Gly Lys Phe Ser Tyr Leu Val Ile His Cys Leu Glu Thr Ser Pro

645 650 655

Arg Phe Arg Asp Arg Val Met Gly Leu Phe Arg Gln Arg Ala Gly Thr

660 665 670

Ala Ser Ala Ser Ser Gly Pro Thr Pro Met Pro Ser Val Ala Lys Val

675 680 685

Gln Ile Ile Glu Tyr Leu Tyr Glu Ala Gly Ser Phe Asp Ala Cys Trp

690 695 700

Arg Leu Leu Val Arg Leu Glu Asp Asp Ile Glu Gly Glu Ile Arg Arg

705 710 715 720

Leu Glu Glu Ala Thr Gly Glu Glu Asn Pro Gln Met His Leu Leu Leu

725 730 735

Lys Leu Leu Ser Val Lys Asn Asp Lys Pro Asn Lys Gly Pro Val Val

740 745 750

Val Pro Ala Gly Leu

755

<210> 8

<211> 718

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 8

Met Ala Gly Thr Arg Ser Ser Arg Pro Gly Ala Thr Ser Phe Ile Gln

1 5 10 15

His Ser Ile Pro Leu Pro Arg Ser Ala Tyr Glu Gly Val Glu Tyr Phe

20 25 30

Cys Arg Phe Arg Pro Arg Ile His Arg Asp Ala Ile Leu Ala Asp Ala

35 40 45

Gly Ser Trp Gln Cys Gln Val Asp Phe Phe Gly Ser Ser Ala Thr Ala

50 55 60

Arg Ala Asp Ser Ile Arg Asn Lys Asn His Thr Ser Tyr Ala Val Gly

65 70 75 80

Cys Ile Asn Pro Val Val Gly Asn Phe Thr Ala Leu Cys Ala Cys Glu

85 90 95

Ala Ile Pro Asp Arg Leu Ala Leu Thr Thr Tyr Met Val Glu Tyr Ala

100 105 110

Tyr Ile His Asp Asp Val Ile Glu Tyr Ala Glu Asn Lys Asp Glu Asp

115 120 125

Arg Asp Asn Val Arg Arg Arg Gln Leu Gln Ala Lys Met Ala Val Glu

130 135 140

Leu Met Asp Ile Asp Lys Val Lys Gly Lys Glu Cys Leu Arg Leu Trp

145 150 155 160

Lys Glu Met Ser Asp Val Phe Val Gln Ile Arg Glu Leu Lys Phe Thr

165 170 175

Lys Leu Asp Asp Tyr Leu Thr Phe Arg Val Ile Asp Ala Gly Cys Pro

180 185 190

Trp Thr Met Ser Leu Leu Cys Phe Ser Met Asp Phe Thr Leu Asn Asp

195 200 205

Asp Glu Val Glu Lys Thr Ala Ala Ile Thr Ser Ala Ala Tyr Asp Gly

210 215 220

Trp Val Leu Val Asn Asp Tyr Phe Ser Trp Glu Lys Glu Trp Lys Asn

225 230 235 240

His Gln Ala Asn Gly Gly Ser Gly Val Ile Ala Asn Ala Ile Phe Leu

245 250 255

Phe Met Arg Trp Tyr Ser Val Asp Ala Val Glu Gly Arg Arg Met Leu

260 265 270

Arg Lys Glu Ile Leu Ala Arg Glu Glu Lys Tyr Cys Lys Ala Lys Glu

275 280 285

Glu Phe Leu Val Ser Gly Asn Val Thr Asp Lys Thr Ser Gln Trp Leu

290 295 300

Glu Leu Leu Asp His Val Thr Ala Gly Asn Phe Ala Trp Ser Met Thr

305 310 315 320

Thr Ala Arg Tyr Gln Leu Gly Gly Lys Asp Ala Tyr Pro Ala Leu Arg

325 330 335

Ala Ala Asn Thr Asp Asn Trp Glu Thr Ser Thr Thr Asp Ser Leu Ser

340 345 350

Asn Pro Ile Ser His Asn Ala Asp Lys Ile Ala Arg Lys Ile Asn Leu

355 360 365

Ile Phe Lys Glu Gln Lys Phe Leu Asp Ala Arg Gly Leu Val Asn His

370 375 380

Thr Glu Asp Tyr Pro Pro Ile Val Leu Thr Ala Gln Val Ser Gln Pro

385 390 395 400

Asp Glu Thr Pro Glu Phe Ile His Ser Gln Val Thr Gln Ala Arg Ser

405 410 415

Phe Thr Gln Tyr Glu Lys Met Ile Leu Gln Pro Gln Asn Tyr Leu Glu

420 425 430

Ser Met Pro Ser Lys Gly Val Arg Asn Ser Val Ile Asp Gly Leu Glu

435 440 445

Met Trp Tyr Gln Val Pro Glu Arg Ser Leu Ala Thr Ile Arg Lys Ile

450 455 460

Val Asn Leu Leu His Ser Ser Ser Leu Met Leu Asp Asp Ile Glu Asp

465 470 475 480

Asn Ser Pro Leu Arg Arg Gly Leu Pro Ala Thr His Thr Val Phe Gly

485 490 495

Ile Ser Gln Thr Ile Asn Ser Ala Asn Leu Leu Met Phe Lys Ala Leu

500 505 510

Lys Ala Ala Glu Ser Leu Ser Pro Ala Ala Val Arg Ile Phe Ile Glu

515 520 525

Arg Ile Ile Glu Gly His Ile Gly Gln Gly Met Glu Leu Tyr Trp Thr

530 535 540

Phe His Thr Glu Ile Pro Thr Glu Glu Glu Tyr Phe Val Met Val Asp

545 550 555 560

Gly Lys Thr Gly Gly Leu Phe Ile Leu Leu Ala Glu Leu Met Arg Ser

565 570 575

Glu Ala Thr Arg His Lys Asp Leu Asp Thr Ser Leu Leu Met Lys Leu

580 585 590

Val Gly Arg Phe Phe Gln Ala Arg Asp Asp Tyr Gln Asn Leu Glu Ser

595 600 605

Ala Gln Tyr Thr Gln Gln Lys Gly Phe Ala Glu Asp Ile Gly Glu Gly

610 615 620

Lys Leu Ser Leu Pro Leu Ile His Ala Leu Gly Ser Lys Thr Pro Gln

625 630 635 640

Arg Gly Arg Leu Met Ser Ile Leu Gln Gln Arg Lys Ser Thr Val Asp

645 650 655

Leu Pro Phe His Ile Arg Lys Leu Ala Leu Asp Asp Ile Lys Ala Thr

660 665 670

Gly Gly Leu Lys Tyr Ala Lys Lys Met Ala Met Ser Leu Gln Asp Ser

675 680 685

Val Asn Glu Thr Leu Thr Gln Tyr Glu Asp Lys Val Gly Ala Lys Asn

690 695 700

Trp Ile Leu Arg Leu Val Gln Lys Arg Leu Glu Leu Glu Val

705 710 715

<210> 9

<211> 712

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 9

Met Ala Glu Glu Met Trp Lys Tyr Ser Ile Pro Ile Asp Pro Glu Thr

1 5 10 15

Val Lys Gln Thr Gly Cys Phe Thr Thr Leu Pro Val Arg Ile Asn Asn

20 25 30

Arg Asp Asp Leu Ala Asn Ala Ala Ser Ser Arg Val Leu Lys Asp Trp

35 40 45

Ala Glu His Thr Gly Asn Gln Asn Ile Asp Pro Asn Arg Val Ser Phe

50 55 60

Ser Pro Val Gly Ser Phe Cys Ser Leu Ile Tyr Cys Glu Thr Ile Pro

65 70 75 80

Glu Arg Leu Asp Ser Ile Ser Tyr Leu Thr Asp Leu Phe Phe Leu Ile

85 90 95

Asp Asp Ala Thr Glu Glu Val Glu Asn Asp Lys Val Ala Gln Glu Gln

100 105 110

Trp Ala Gly Phe Ser Gly Ala Met Thr Asp Ser Leu Gly Glu Thr Pro

115 120 125

Gln Arg Asp His Asp Leu Asp Ser Met Lys Lys Lys Lys Leu Val Ala

130 135 140

Arg Val Met Leu Asp Phe Met Arg Leu Asp Ser Ala Leu Gly Leu Asp

145 150 155 160

Leu Val Lys Ser Cys Lys Ala Gly Trp Thr Pro Leu Ala Ala Gly Val

165 170 175

Glu Trp Glu Thr Met Glu Asp Tyr Leu His Phe Arg Arg Leu Ser Ala

180 185 190

Gly Leu Asp Ile Tyr Trp Thr Lys Thr Val Phe Gly Leu Gly Glu Lys

195 200 205

Leu Ser Asp Asp Glu Glu Lys Leu Ile Arg Pro Leu Val Trp Ala Ala

210 215 220

Glu Lys Ala Ala Met Leu Asn Asn Asp Tyr Trp Ser Trp Asp Ile Glu

225 230 235 240

Tyr Phe Gln Ala Asn Gln Lys Ile Asp Met Leu Thr Asn Ala Val Ala

245 250 255

Val Leu Met Arg Lys Glu Gly Ile Thr Ala Glu Glu Gly Lys Asn Arg

260 265 270

Ile Lys Asn Leu Ile Leu Gly Tyr Glu Ala Glu Tyr Ser Gln Leu Arg

275 280 285

Ala Gln Phe Tyr Glu Ser His Pro Ser Ala Arg Leu Tyr Leu Arg Lys

290 295 300

Arg Val Glu Leu Ala Gly Ser Met Ala Ala Gly Val Ser Phe Trp Ser

305 310 315 320

Ala Asn Ser Pro Arg Tyr His Leu Pro Thr Asp Lys Pro Arg Pro Ser

325 330 335

Val Val Pro Val Glu Val Lys Phe Glu Glu Met Thr Leu Lys Ser Ser

340 345 350

Ala Glu Ser Val Ala Ser Thr Glu Ser Glu Asn Ser Met Ala Ala Thr

355 360 365

Ser Gly Ser Gly Thr Glu Leu Thr Ser Gln Ser Ser His Ser Asp Gly

370 375 380

Leu Arg Lys Arg Tyr Phe Asp Val Pro Lys Leu Gly Pro Ala Ala Ile

385 390 395 400

Asn Glu Pro Ile Asp Tyr Val Ser Gly Met Pro Ser Lys Gly Val Arg

405 410 415

Ser Ser Leu Ile Asp Ala Met Asn Gln Trp Cys Gln Val Pro Ser Ser

420 425 430

Gln Leu Ala Val Val Lys Arg Val Ile Asp Leu Leu His Asn Ser Ser

435 440 445

Leu Ile Leu Asp Asp Ile Gln Asp Asp Ser Pro Met Arg Arg Gly Lys

450 455 460

Thr Ala Thr His Leu Ile Phe Gly Glu Ala Gln Ser Ile Asn Ser Ala

465 470 475 480

Thr Tyr Leu Tyr Val Arg Val Val Gln Glu Val His Ala Thr Gly Asn

485 490 495

Ala Ala Leu Met Lys Val Leu Leu Glu Glu Leu Asp Asp Leu His Val

500 505 510

Gly Gln Ser Trp Asp Leu Tyr Trp Lys Tyr Asn Leu Lys Trp Pro Thr

515 520 525

Glu Gly Glu Tyr Phe Ser Met Ile Asp Leu Lys Thr Gly Gly Leu Phe

530 535 540

Arg Met Leu Val Arg Met Met His Ala Leu Cys His Pro Glu Cys Lys

545 550 555 560

Glu Glu Phe Ala Cys Asp Ala Leu Val Ser Met Val Ser Arg Phe Phe

565 570 575

Gln Val Arg Asp Asp Tyr Leu Asn Leu Asn Ser Arg Glu Tyr Ser Asn

580 585 590

Gln Lys Gly Trp Cys Glu Asp Leu Asp Glu Gly Lys Phe Ser Tyr Leu

595 600 605

Ile Ile His Cys Leu Glu Asn Ser Pro Arg Tyr Arg Asp Arg Ile Met

610 615 620

Gly Leu Phe Arg Gln Arg Thr Gly Cys Ser Gly Pro Met Pro Ser Val

625 630 635 640

Gly Lys Val Gln Ile Ile Glu Tyr Leu Gln Glu Ala Gly Ser Phe Asp

645 650 655

Ala Cys Trp Glu Leu Leu Asn Lys Leu Glu Asp Glu Ile Glu Ala Glu

660 665 670

Ile Arg Arg Leu Glu Ala Val Thr Gly Glu Glu Asn Pro Gln Met His

675 680 685

Leu Leu Leu Lys Leu Leu Ser Val Lys Asn Glu Lys Pro Asn Lys Gly

690 695 700

Pro Val Ile Val Pro Leu Gly Val

705 710

<210> 10

<211> 718

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 10

Met Cys Thr Met Asp Asp His Thr Ser Glu Leu Leu Asp Ile Ser Gln

1 5 10 15

Phe Asp Thr Gln Gly Phe Cys Pro Gly Tyr Pro Leu Arg Arg His Arg

20 25 30

Tyr Glu Ser Leu Ala Asn Ser Gly Cys Tyr Glu Ala Arg Gln Asp Trp

35 40 45

Ile His Tyr Val Gly Pro Ala Glu Glu Phe Gly Ser Cys Asp Pro Thr

50 55 60

Asn Gly Asn Phe Thr Ala Leu Val Leu Pro Leu Thr Lys Pro Glu Arg

65 70 75 80

Leu His Leu Val Ala Tyr Ile Ile Glu Cys Glu Phe Ile Arg Val Leu

85 90 95

Tyr Pro Tyr Ala Phe Leu His Asp Asn Val Val Glu Ala Val Met Pro

100 105 110

Asp Ser Ser Thr Asn Asn Gly Asp Asp Phe His Leu Gly Glu Ala Glu

115 120 125

Lys Arg Gln Arg Asn Val Arg Met Gly Arg Lys Gln Ile Gln Ala Lys

130 135 140

Met Met Tyr Gln Leu Ala Arg Thr Asp Lys Arg Cys Ala Glu Arg Val

145 150 155 160

Lys Arg Val Trp Lys Thr Met Leu Ser Thr Thr Leu Lys His Lys Ser

165 170 175

Asp Ser Phe Ala Ser Leu Glu Glu Tyr Leu Asn Tyr Arg Ile Ile Asp

180 185 190

Thr Gly Gly Tyr Trp Val Glu Ser Val Met Leu Phe Gly Met Gly Met

195 200 205

Thr Leu Thr Glu Glu Glu Asp Ala Gln Leu Ala Glu Val Val Arg Pro

210 215 220

Cys Tyr Ala Ser Leu Ala Leu Ala Asn Asp Tyr Phe Ser Phe Asp Arg

225 230 235 240

Glu Trp Glu Glu Ala Gln Asn Gly Gly Pro Pro Pro Thr Asn Ala Val

245 250 255

Trp Leu Tyr Met Arg Trp Asn Gly Ile Pro Met Pro Ala Ala Lys Lys

260 265 270

Leu Val Arg Glu Ala Ser Asn Arg Tyr Glu Ala Arg Phe Leu Glu Leu

275 280 285

Cys Asp Ser Phe Arg Arg Asn Asn Pro Leu Tyr Ser Glu Lys Leu Asp

290 295 300

Lys Tyr Leu Arg Gly Leu Ala Tyr Gln Ile Ser Gly Asn Val Val Trp

305 310 315 320

Ser Leu Thr Cys Pro Arg Tyr His Pro Glu Phe Arg Tyr Asp Pro Asn

325 330 335

Ala Gly Met Glu Asp Leu Leu Thr Ala Gln Ala Arg Gly Asp Gly Gly

340 345 350

Ala Val Ser Gly Gly Glu Ala Asp Tyr Thr Ala Glu His Arg Gln Ser

355 360 365

Ile Ile Ser Leu Glu Ser Gln His Thr Ala Ser Ser Thr Arg Tyr Ser

370 375 380

Ala Ser Asp Trp Gln Ser Ser Arg Ser Ser Ser Phe Ser Glu Ile Ser

385 390 395 400

Val Asp Gly Glu Asp Gly Glu Thr His Ala Val Lys Leu Pro Ala Glu

405 410 415

Gln Gly Leu Asp Thr Lys His Val Ala Ala Pro Phe Asp Tyr Val Ala

420 425 430

Ser Leu Pro Ser Lys Gly Val Arg Thr Thr Leu Ile Asp Ala Leu Asn

435 440 445

Leu Trp Cys Asp Leu Pro Asp Thr Thr Leu Ala Gly Ile Lys Glu Val

450 455 460

Val Asp Lys Leu His Thr Ala Ser Leu Met Phe Asp Asp Ile Glu Asp

465 470 475 480

Gly Ser Glu Leu Arg Arg Gly Asn Pro Ala Ala His Ala Val Tyr Gly

485 490 495

Val Pro Gln Thr Ile Asn Ala Ala Ser Phe Ala Ile Val Asp Ala Val

500 505 510

Ser Lys Ala Gln Asp Ile Pro Val Thr Gly Ala Val Asp Ile Val Leu

515 520 525

Glu Gln Leu Arg Asp Leu His Ile Gly Gln Ser Tyr Asp Ile His Trp

530 535 540

Thr Arg His Ser Ser Cys Pro Ser Glu Thr Glu Tyr Leu Glu Met Val

545 550 555 560

Ala Lys Lys Thr Gly Gly Leu Phe Lys Leu Ile Ser Arg Leu Met Thr

565 570 575

Thr Gly Leu Pro Asp Ile Thr Lys Ser Thr Ile Asp Asp Leu Val Thr

580 585 590

Gln Leu Gly Ile Tyr Phe Gln Ile Arg Asp Asp Phe Lys Asn Leu Asn

595 600 605

Ser Asp Glu Tyr Ala Ser Gln Lys Gly Phe Cys Glu Asp Leu Asp Glu

610 615 620

Gly Lys Phe Ser Phe Pro Leu Val His Tyr Leu Thr Lys Gly Gln Glu

625 630 635 640

Ser Pro Pro Phe Met Leu Arg Glu Ile Met Gln Gln Arg Arg Glu Ser

645 650 655

Lys Gly Leu Asn Val Pro Ser Lys Met Leu Ala Leu Gln Gln Leu Lys

660 665 670

Lys Ser Gly Ser Leu Glu Tyr Thr His Arg Thr Leu Lys Arg Leu Glu

675 680 685

Val Gln Ile Asn Gln Ile Ile Gly Arg Leu Glu Arg Ile Thr Gly Lys

690 695 700

Asp Asn Trp Val Leu Arg Leu Cys Met Gly Glu Leu Thr Val

705 710 715

<210> 11

<211> 703

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 11

Met Ile Ser Ile Ala Pro Phe Pro Asp Glu Asp Gln Phe Ser Val Leu

1 5 10 15

Val Pro Arg Asp Thr Pro Asp Leu His Gly Phe Cys His Gly Tyr Pro

20 25 30

Leu Arg Arg His Lys Ala Glu His Lys Ala Asn Glu Gly Ser Leu Gln

35 40 45

Leu Arg Ala Asp Trp Glu Lys Tyr Ile Gly Pro Ile Glu Arg Trp Gly

50 55 60

Ser Cys Asn Pro Trp Glu Gly His Phe Gly Ala Val Val Leu Pro Cys

65 70 75 80

Cys Arg Pro Glu Arg Met Ala Ile Val Asn Tyr Ile Phe Glu Tyr Ala

85 90 95

Phe Met Tyr Asp Asn Val Val Glu Ser Ala Ala Gln Ser Thr Ile Asn

100 105 110

Ala His Ala Asp Asp Ile Ala Leu Asp Glu Thr Glu Tyr Arg Thr Val

115 120 125

Arg Ser Val Thr Gly Thr Lys Gln Val Gln Ser Lys Met Leu Leu Glu

130 135 140

Leu Phe Ala Leu Asp Pro Ala Cys Ala Gln Val Val Leu Asp Ser Trp

145 150 155 160

Lys Thr Met Ile Asp Thr Thr Ala Thr Lys Asp Lys Thr Arg Ala Phe

165 170 175

Gln Asn Trp Glu Glu Tyr Val Asp Tyr Arg Ile Ile Asp Thr Gly Ala

180 185 190

Pro Phe Val Asp Met Leu Met Arg Phe Gly Met Gly Phe Val Leu Thr

195 200 205

Pro Glu Glu Gln Ala Ser Ile Glu Pro Val Val Arg Pro Cys Tyr Ala

210 215 220

Ala Leu Gly Leu Ala Asn Asp Tyr Phe Ser Phe Asp Val Glu Trp Asp

225 230 235 240

Glu Phe Gln His Ala Glu Glu Thr Thr Met Thr Asn Ala Val Trp Leu

245 250 255

Cys Met His Trp His Gly Val Asp Val Pro Ala Ala Lys Glu Ile Val

260 265 270

Arg Arg Val Thr Asn Asn Phe Glu Arg Glu Phe Gln Gln Arg Val Ala

275 280 285

Glu Tyr Val Ala Gly Asp Gly Arg Glu Asn Arg Lys Val Gln Val Tyr

290 295 300

Leu Arg Ala Leu Gly Tyr Gln Ile Pro Gly Asn Val Ala Trp Ser Leu

305 310 315 320

Arg Cys Pro Arg Tyr His Pro His Leu Val Glu Glu Ala Gly Arg Leu

325 330 335

Leu Gln Gln Ser Met Gln Thr Ala Ser Asp Glu Gly Cys Val Ala Pro

340 345 350

Val Gln Val Gln Ala Lys Arg Ala Ser Val Ser Glu Glu Ser Glu Ser

355 360 365

Ser Glu Ser Ser Val Trp Ser Gly Pro Asn Ser Pro Arg Ser Ser Ile

370 375 380

Ser Ser Ala Ser Glu Glu Glu Glu Ala Arg Glu Val Glu Glu Val Lys

385 390 395 400

Leu Gly Ala Glu His Leu Leu Asn Pro Ala Glu Tyr Ile Ala Ser Leu

405 410 415

Pro Ser Lys Gly Val Arg Glu Ala Phe Ile Asp Ala Leu Asn Val Trp

420 425 430

Leu Met Leu Pro Asp Arg Gln Val Lys Leu Leu Lys Ser Ile Ala Lys

435 440 445

Thr Leu His Asn Ala Ser Leu Met Leu Asp Asp Ile Glu Asp Ser Ser

450 455 460

Pro Leu Arg Arg Gly Gln Pro Ala Thr His Thr Val Tyr Gly Val Gly

465 470 475 480

Pro Thr Ile Asn Ser Ala Asn Tyr Leu Leu Leu Gln Ala Leu Asp Glu

485 490 495

Val Arg Gly Leu Asp Asp Ser Asp Gly Gln Cys Leu Asp Ile Tyr Thr

500 505 510

Glu Glu Met Arg Asn Leu Phe Thr Gly Gln Ser Tyr Asp Val Tyr Trp

515 520 525

Thr Arg Glu Ala Val Cys Pro Ser Glu Ala Glu Tyr Met Glu Met Ile

530 535 540

Arg Gln Lys Thr Gly Gly Leu Phe Arg Leu Leu Ala Arg Leu Met Ile

545 550 555 560

Ala Thr Ala Pro Ala Gln Arg Asn Arg Asp Ile Pro Leu Leu Pro Leu

565 570 575

Ile Asp Lys Leu Gly Glu Tyr Phe Gln Ile Arg Asp Asp Tyr Lys Asn

580 585 590

Leu Thr Glu Glu Tyr Thr Asp Gln Lys Gly Phe Cys Glu Asp Leu Asp

595 600 605

Glu Gly Lys Phe Ser Phe Pro Leu Ile His Ala Leu Arg Ala Gln Glu

610 615 620

Arg Glu Lys Asn Gly Arg Leu Trp Glu Met Leu Glu Gln Gly Arg Ala

625 630 635 640

Gly Gly Thr Met Glu Leu Ala Arg Lys Arg Glu Val Leu Asp Ile Leu

645 650 655

Gln Asp Ser Gly Ser Met Glu Tyr Thr Arg Arg Thr Leu Gln Gly Ile

660 665 670

Met Gly Glu Ile His Gly Gly Met Gly Arg Val Glu Arg Glu Thr Gly

675 680 685

Gly Val Asn Trp Val Leu Arg Leu Leu Val Met Arg Leu Glu Val

690 695 700

<210> 12

<211> 7327

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

ggccgcttca ccgattctga gcgaatcaca ggtgagaaat ttggattcga aataaaccta 60

aaaaaactat ccaataaggc ttccataggc ttcgtatttc ccgaccattc caattggaaa 120

aattgagcgc tgtcttccat gatcttagat aaagccttaa tacttggctc atttccattt 180

gaggttagct ctagcaactg gtaaagcatt tgaagaccag ttggatcatc tctatgctgc 240

ctatagtaag tggaaaataa agtagtaata acgatacctg tcacgttcga aacgagatta 300

ttgttcgaat tataaaggcc atgtataatg tttgatttga cgtatcctaa gtcgtggcta 360

ttgctcttaa ttaaattgtt tccccctagc attgaatttt tcaacagcat accggcggta 420

gctctattgt tctgaagatc ctgtagggag tagtgttgct tcaatacatc atcagattcg 480

ccttcaatta aaatataaca caaataattg agaaattcag gttgcagctg gaagttctcc 540

atagcttcca ttgcgttatt acgaatctct ggatttggtg acatacagtt ctgtaaaaga 600

gttgctagtt gcaacacata gtcttcggcg ggcttccatg tcgatgccat ctttattcac 660

ttaactactg ctaacaattc tggaaaccaa agactgcgga atattctgat atgtattact 720

actattcgct gctcttctgc ataattaata ctgaaaagtt tttcatactt ttaaacataa 780

ccttttttta agcaaaactc tatgacccgg attagaaaac tacgaaaaga gggtaataac 840

ataggtgcag gatttccatc gataacgacg ccgacaatga gccttgctgc aacatccaat 900

taggactaat aactatcgta ggaatttcta cgtaataaac ttcaacagag cctaaaattt 960

gaaaataaat aatctagagg ggaaacttaa agaaattcta ttcttgtcaa taaagtggaa 1020

atctgtcaga tgtcacagtt tctttatttg tgacacatat tttcaacata aattcaggca 1080

ttagtgctgt aagcacaaaa agttgtggcg atatgaatat tccagatttt acttacaagc 1140

tgcattgtag tcttacaatt ctttttttct tttttttttt ttatggaaag gaccactctt 1200

acataactag aatagcatta agaatcagat ttacagataa agatgacatt attttatata 1260

tatattgtca ctccgttcaa gtcgacaacc aataaaaaat ttaaaaaaag ccaggcagtt 1320

aatagaaaaa atatgatatg aatgaatatt ccactttctt ttcacagctt gtctgtaagc 1380

ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 1440

ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata aacgacatta 1500

ctatatatat aatataggaa gcatttaata gacagcatcg taatatatgt gtactttgca 1560

gttatgacgc cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct 1620

tacgtacaat cttgatccgg agcttttctt tttttgccga ttaagaatta attcggtcga 1680

aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata 1740

cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc 1800

tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga 1860

ttccgatgct gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc 1920

ggttattgca aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc 1980

gaaatacttg gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa 2040

tgattacggc attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga 2100

gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact 2160

cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg 2220

gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc 2280

cgaaagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct 2340

tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa 2400

agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta 2460

gtatttattt aagtattgtt tgtgcacttg cctatgcggt gtgaaatacc gcacagatgc 2520

gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta aaattcgcgt 2580

taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 2640

ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 2700

cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 2760

gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 2820

taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 2880

tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 2940

cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 3000

cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 3060

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 3120

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact 3180

cactataggg cgaattgggt actacacggt ccaatggata aacatttttt atcaacacta 3240

tgatatataa atataatagt ttttcgtata tatattcctt tttttggtca atttttgaaa 3300

ttttcgtaga aaagggagag acaaaacaca ttatatcaat gaaaacgtac aaaaagtaga 3360

taaagtcagt gcttaaacac gtcttttcct taaaaatact ttattatttt tattttattg 3420

agagggtggt ttaaaaatag aaatagagag agaggtacat acataaacat acgcgcacaa 3480

aagcagagat taggatttaa tgcaggtgac ggacccatct ttcaaacgat ttatatcagt 3540

ggcgtccaaa ttgttaggtt ttgttggttc agcaggtttc ctgttgtggg tcatatgact 3600

ttgaaccaaa tggccggctg ctagggcagc acataaggat aattcacctg ccaagacggc 3660

acaggcaact attcttgcta attgacgtgc gttggtacca ggagcggtag catgcgggcc 3720

tcttacacct aataagtcca acatggcacc ttgtggttct agaacagtac caccaccgat 3780

ggtacctact tcgatggatg gcatggatac ggaaattctc aaatcaccgt ccacttcttt 3840

catcaatgtt atacagttgg aactttcaac attttgtgca ggatcttgtc ctaatgccaa 3900

gaaaacagct gtcactaaat tagctgcatg tgcgttaaat ccaccaacag acccagccat 3960

tgcagatcca accaaattct tagcaatgtt caactcaacc aatgcggaaa catcactttt 4020

taacactttt ctgacaacat caccaggaat agtagcttct gcgacgacac tcttaccacg 4080

accttcgatc cagttgatgg cagctggttt tttgtcggta cagtagttac cagaaacgga 4140

gacaacctcc atatcttccc agccatactc ttctaccatt tgctttaatg agtattcgac 4200

acctttagaa atcatattca tacccattgc gtcaccagta gttgttctaa atctcatgaa 4260

gagtaaatct cctgctagac aagtttgaat atgttgcaga cgtgcaaatc ttgatgtaga 4320

gttaaaagct tttttaattg cgttttgtcc ctcttctgag tctaaccata tcttacaggc 4380

accagatctt ttcaaagttg ggaaacggac tactgggcct cttgtcatac catccttagt 4440

taaaacagtt gttgcaccac cgccagcatt gattgcctta cagccacgca tggcagaagc 4500

taccaaacaa ccctctgtag ttgccattgg tatatgataa gatgtaccat cgataaccaa 4560

ggggcctata acaccaacgg gcaaaggcat gtaacctata acattttcac aacaagcgcc 4620

aaatacgcgg tcgtagtcat aatttttata tggtaaacga tcagatgcta atacaggagc 4680

ttctgccaaa attgaaagag ccttcctacg taccgcaacc gctctcgtag tatcacctaa 4740

ttttttctcc aaagcgtaca aaggtaactt accgtgaata accaaggcag cgacctcttt 4800

gttcttcaat tgttttgtat ttccactact taataatgct tctaattctt ctaaaggacg 4860

tattttctta tccaagcttt caatatcgcg ggaatcatct tcctcactag atgatgaagg 4920

tcctgatgag ctcgattgcg cagatgataa acttttgact ttcgatccag aaatgactgt 4980

tttattggtt aaaaccattt atattgaatt ttcaaaaatt cttacttttt ttttggatgg 5040

acgcaaagaa gtttaataat catattacat ggcaatacca ccatatacat atccatatct 5100

aatcttactt atatgttgtg gaaatgtaaa gagccccatt atcttagcct aaaaaaacct 5160

tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa gtacggatta 5220

gaagccgccg agcgggcgac agccctccga cggaagactc tcctccgtgc gtcctggtct 5280

tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa 5340

gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc 5400

acaaaccttc aaatcaacga atcaaattaa caaccatagg ataataatgc gattagtttt 5460

ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat 5520

atataaatgc aaaagctgca taaccacttt aactaatact ttcaacattt tcggtttgta 5580

ttacttctta ttcaaatgtc ataaaagtat caacaaaaaa ttgttaatat acctctatac 5640

tttaacgtca aggagaaaaa actatagcgc aatgattgaa tagtcaaaga tttttttttt 5700

ttaatttttt ttttttcata gaacttttta tttaaataaa tcacgtctat atatgtatca 5760

gtataacgta aaaaaaaaaa caccgtcagt taaacaaaac ataaataaaa aaaaaaagaa 5820

gtgtcaaatc aagtgtcaaa tgtatacttc ttttttttac tttgttcaga acaacttctc 5880

atttttttct actcataact ttagcatcac aaagtacaca ataataacga gtagtaacac 5940

ttttatagtt catacatgct tcaactactt aataaatgat tgtatgataa tgttttcaat 6000

gtaagagatt tcgattatcc acaaactttg aaacacaggg acacaattct tgatatgctt 6060

tcaaccgctg cgttttggat acctattctt gacataatat gactaccatt ttgttattgt 6120

acgtggggca gttgacgtct tatcatatgt caaagtcatt tgcgaagttc ttggcaagtt 6180

gccaactgac gagatgcagt aaaaagagat tgccgtcttg aaactttttg tccttttttt 6240

tttccgggga ctctacgaga accctttgtc ctactgatta attttgtact gaatttggac 6300

aattcagatt ttagtagaca agcgcgagga ggaaaagaaa tgacagaaaa attccgatgg 6360

acaagaagat aggaaaaaaa aaaagctttc accgatttcc tagaccggaa aaaagtcgta 6420

tgacatcaga atgaaaaatt ttcaagttag acaaggacaa aatcaggaca aattgtaaag 6480

atatgataaa tcatttgatt cagcgccaat ttgccctttt ccattttcca ttaaatctct 6540

gttctctctt acttatatga tgattaggta tcatctgtat aaaactcctt tcttaatttc 6600

actctaaagc ataccccata gagaagatct ttcggttcga agacattcct acgcataata 6660

agaataggag ggaataatgc cagacaatct atcattacat ttaagcggct cttcaaaaag 6720

attgaactct cgccaactta tggaatcttc caatgagacc tttgcgccaa ataatgtgga 6780

tttggaaaaa gagtataagt catctcagag taatataact accgaagttt atgaggcatc 6840

gagctttgaa gaaaaagtaa gctcagaaaa acctcaatac agctcattct ggaagaaaat 6900

ctattatgaa tatgtggtcg ttgacaaatc aatcttgggt gtttctattc tggattcatt 6960

tatgtacaac caggacttga agcccgtcga aaaagaaagg cgggtttggt cctggtacaa 7020

ttattgttac ttctggcttg ctgaatgttt caatatcaac acttggcaaa ttgcagctac 7080

aggtctacaa ctgggtctaa attggtggca gtgttggata acaatttgga ttgggtacgg 7140

tttcgttggt gcttttgttg ttttggcctc tagagttgga tctgcttatc atttgtcatt 7200

ccctatatca tctagagcat cattcggtat tttcttctct ttatggcctg ttattaatag 7260

agtcgtcatg gccatcgttt ggtatagtgt ccaagcttat attgcggcaa ctcccgtatc 7320

attaagc 7327

<210> 13

<211> 10833

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

ggtggcaagc ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag 60

aagtttaata atcatattac atggcattac caccatatac atatccatat acatatccat 120

atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa 180

accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg 240

attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc 300

gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa 360

taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg 420

ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag 480

ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac 540

agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt 600

tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct 660

atactttaac gtcaaggaga aaaaactata gtcgactcat gtaattagtt atgtcacgct 720

tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg 780

aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 840

ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa 900

ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc 960

gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 1020

ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 1080

gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 1140

cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 1200

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 1260

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 1320

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 1380

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 1440

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 1500

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 1560

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat 1620

tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta 1680

aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta 1740

gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc 1800

ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga 1860

tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc 1920

atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat 1980

gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt 2040

acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa 2100

aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc 2160

tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc 2220

cgcagaatac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag 2280

taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa 2340

atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac 2400

taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt 2460

ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc 2520

cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag 2580

ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac 2640

caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa 2700

aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg 2760

aaaagctgtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2820

gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2880

cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2940

atcaccgaaa cgcgcgaaag agatggagga acgggaaaaa gttagttgtg gtgataggtg 3000

gcaagtggta ttccgtaaga acaacaagaa aagcatttca tattatggct gaactgagcg 3060

aacaagtgca aaatttaagc atcaacgaca acaacgagaa tggttatgtt cctcctcact 3120

taagaggaaa accaagaagt gccagaaata acagtagcaa ctacaataac aacaacggcg 3180

gctacaacgg tggccgtggc ggtggcagct tctttagcaa caaccgtcgt ggtggttacg 3240

gcaacggtgg tttcttcggt ggaaacaacg gtggcagcag atctaacggc cgttctggtg 3300

gtagatggat cgatggcaaa catgtcccag ctccaagaaa cgaaaaggcc gagatcgcca 3360

tatttggtgt ccccgaggat ccaaatttcc aatcttctgg tattaacttc gataactacg 3420

atgatattcc agtggacgcc tctggtaagg atgttcctga accaatcaca gaatttacct 3480

cacctccatt ggacggattg ttattggaaa acatcaaatt ggcccgtttc accaagccaa 3540

cacctgtgca aaaatactcc gtccctatcg ttgccaacgg cagagatttg atggcctgtg 3600

cgcagaccgg ttctggtaag actggtgggt ttttattccc agtgttgtcc gaatcattta 3660

agactggacc atctcctcaa ccagagtctc aaggctcctt ttaccaaaga aaggcctacc 3720

caactgctgt cattatggct ccaactagag agttggccac ccaaattttc gatgaagcca 3780

agaaatttac ttatagatcc tgggtcaagg cctgcgtcgt ctacggtggt tctccaattg 3840

gtaaccaact aagagaaatt gaacgtggtt gcgatctttt agtcgctact ccaggtcgtt 3900

tgaatgactt gttggaacgt ggtaaaattt ctttggccaa cgtcaagtat ttggttctag 3960

atgaagctga tagaatgttg gatatgggtt tcgaacctca aattagacat attgtcgaag 4020

actgtgatat gactcctgtt ggtgaaagac aaactctgat gttctcagct acttttcccg 4080

ctgatatcca acatttggcc cgtgatttct taagtgacta catctttttg tctgttggta 4140

gagtcggttc tacttcagaa aacattactc aaaaagtctt atacgttgaa aatcaagata 4200

agaagtcagc cttattggat ctattgtctg catccactgc tcgaggcttt tgttcccttt 4260

agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 4320

gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg 4380

gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4440

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4500

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4560

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4620

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4680

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4740

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4800

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4860

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4920

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4980

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5040

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5100

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5160

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5220

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5280

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5340

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5400

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5460

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5520

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5580

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5640

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5700

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5760

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5820

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 5880

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 5940

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6000

ctggtgaata ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6060

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6120

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6180

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6240

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6300

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 6360

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6420

gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa 6480

aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa 6540

cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa 6600

aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta 6660

cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt 6720

tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt 6780

tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc 6840

gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac 6900

tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag 6960

gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga 7020

tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct 7080

atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata 7140

gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag 7200

aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat 7260

atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc 7320

gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt 7380

cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa 7440

cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga 7500

gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata 7560

tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt 7620

ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg 7680

gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca 7740

attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa 7800

accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 7860

gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 7920

gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 7980

ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact cgagtagtga 8040

gtcaacaatg ggttctgggg cccgattgcc tttctcaatg ccaccaaagg gaatttcgac 8100

gaagaagtca ctcctcatct tcaaattcgt tcttacgccc tggctttcgt tccccaccac 8160

tagaacaaca ggcagctcgt tacataatcc gttcaaatcg tgcatgctaa tagtttttcc 8220

aacagtgtat ttttctgacg tggcattagc taagtggctt gtaataaacg tccagccacc 8280

catttcttgt gatttagtaa aaaactctaa cggtttatca acgtaaaata tgggcagaag 8340

ttcgagggcc ccactgcttg tcttggacac cacaggcgtc aaaggagagc agtttcttct 8400

cgacatcaca atgaagtcaa cccccaggaa gtaagcgctt ctaataatgg caccgatatt 8460

gtgagggtca gttatttcat ccagatataa cccgagagga aacttcttag cgtctgtttt 8520

cgtaccataa ggcagttcat gaggtatatt ttcgttattg aagcccagct cgtgaatgct 8580

taatgctgct gaactggtgt ccatgtcgcc taggtacgca atctccacag gctgcaaagg 8640

ttttgtctca agagcaatgt tattgtgcac cccgtaattg gtcaacaagt ttaatctgtg 8700

cttgtccacc agctctgtcg taaccttcag ttcatcgact atctgaagaa atttactagg 8760

aatagtgcca tggtacagca accgagaatg gcaatttcta ctcgggttca gcaacgctgc 8820

ataaacgctg ttggtgccgt agacatattc gaagatagga ttatcattca taagtttcag 8880

agcaatgtcc ttattctgga acttggattt atggctcttt tggtttaatt tcgcctgatt 8940

cttgatctcc tttagcttct cgacgtgggc ctttttcttg ccatatggat ccgctgcacg 9000

gtcctgttcc ctagcatgta cgtgagcgta tttcctttta aaccacgacg ctttgtcttc 9060

attcaacgtt tcccattgtt tttttctact attgctttgc tgtgggaaaa acttatcgaa 9120

agatgacgac tttttcttaa ttctcgtttt aagagcttgg tgagcgctag gagtcactgc 9180

caggtatcgt ttgaacacgg cattagtcag ggaagtcata acacagtcct ttcccgcaat 9240

tttctttttc tattactctt ggcctcctct agtacactct atattttttt atgcctcggt 9300

aatgattttc attttttttt ttccacctag cggatgactc tttttttttc ttagcgattg 9360

gcattatcac ataatggagg gagcagtaaa ggggcgatca gagttgaaaa aaaaatgctg 9420

ccgtaaaaaa agcatgcacg tatacacttg agtaaatata tttatgtatt catatctcaa 9480

gataagatca tgatacataa aagcgatata acgtcttgaa gctcaggtaa ggggctagta 9540

gatgcaatga atgacgatga agatagagcc caactgaagg ctaggctgtg gatccgtgtg 9600

gaagaacgat tacaacaggt gttgtcctct gaggacataa aatacacacc gagattcatc 9660

aactcattgc tggagttagc atatctacaa ttgggtgaaa tggggagcga tttgcaggca 9720

tttgctcggc atgccggtag aggtgtggtc aataagagcg acctcatgct atacctgaga 9780

aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta 9840

agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat 9900

cataaatcat aagaaattcg cgaattctta atgatctctt gcagcaacta attcacaaat 9960

gtatgccaat gcagcaccat caacatcagc atttctcaaa tgtctttgtg cagcttcgat 10020

atggaaagct aatttttcct ttgcaccagc caatgataac aatgctggat atgtagcctt 10080

attgtttgat tgatcagaac caactggttt accaatcttt tcttctgcac cttcaatatc 10140

taaaatatca tctctgattt ggaaagctaa acccaaatgt gcagcaaatt catccaattc 10200

tcttgtttgt cttgcatcag caccaccaat taatgcacca gcatgaactg agtattgcaa 10260

cattttacca gtcttatgtc tatgaatata ttctaattca gacaatgtca aagttttacc 10320

ttcaccttcc atatctgcag cttgacctgc agccataccc tctggacctg cagctttagc 10380

caatctttcg atcaatctca atctaacaga tggtggtatt ctttcatcat cgatttcagt 10440

gatcaattgg aatgcataag ctaacaaacc gtcaccagcc aaaattgcca tagcttcacc 10500

gaaaacctta tggtttgttg gtttacctct tctcaaatca tcattatcca ttgatggcaa 10560

atcatcatga atcaaagaag cagtatggat catttcgatt gcacaagcaa ctggtaaacc 10620

aactgctgga tctttaccca aagctctaac tgttgataac aacaacaatg gtctaattct 10680

tttaccacct gcttctaaag agtatgccat agctttcttc aatttagctg gaccttccaa 10740

tctttcgatg tatcttgaca atgcagtttc aacagcttgc ttttgttcgt tcaagaattg 10800

ttcaacagac aattgagctg atccagtact gcc 10833

<210> 14

<211> 2145

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

atggaatcat tatgggaaca ttctgttcca gttgatcatg aagttgcttt atcaactggt 60

tgtttcacat ctttgccaat cagaatacat caaagaaacg atttggcaga tgatgctact 120

aagcaatcaa tcagagattg gggttcttat gttggtgacg gttgggaaca aagatcaggt 180

tcttcatggt ctccagttgg taattggggt gcttttattt tcccagaatc tttaccagaa 240

agattgggtg ttattacata tttggcaaac atgggtaaca tccatgatga tttgtgtgat 300

gatttgactt acgatgaagc attggaagaa cataactcat tgtctcaagc aatggaaatc 360

tcaacatctg gtccacatca aggtactcaa aacttcatga gatcaatgaa gatgaagaaa 420

tacatctcta aatacttgtt ggaagcattg gaaattgata gaccaagagc attgagaatg 480

atcaactcat acagatctaa gtggttggat gttatggaaa gaaacaacgt taacgatatc 540

gaatcattag atgaatactt gatttttaga aatttgaacg gtggtatgga agcattttgg 600

tctatggttg aatttggtat ggcagttgat gtttcagaat ctgataagaa aagaattaga 660

ccaatctttg ctgcagctga atcagctttg gttttaacaa acgattactg gtcttgggat 720

agagaatggt ggcaagcaca aagagctaaa gatccaagaa tcgttaacgc tgttcatttg 780

tttatgagaa ctgaaggttt ggcaatctat caagcaagag aagctgttag agatagaatt 840

ttggcttatg aagctgaatt tttgagattg aaggctgaat tttacgttca aaatccaaac 900

gcagctgttc atttgaagag atacatcgaa ttgtgtggtg ttattacagc tggtaatcat 960

tactggtgtg caaattgtcc aagacatcat tcttggagaa atgaagatat tccaccaatc 1020

gaaagatcat tttctttttc ttcagaagat actatcccag atgattgtac attgtcacca 1080

gcagctacta tttcttcatc tgtttcttac aagtcatctc caactacagt tttgacatca 1140

tctaagtcta tcggtgttat tgcaactgct gatggtgacg aaaagccaca aagattgtca 1200

gattctgttt tgcaaggtcc atgtcaatac attagatcaa tgccatctaa aggtttgaga 1260

catttgtttg cagaagcatt gagacaatgg ttgttagttg aagatgcttc attgcatcaa 1320

attaaaaata tcatcgattt gttgcataat tcatctttga tcttggatga tatcgaagat 1380

gattctccat taagaagagg tttgccagca acacacatgg tttttggtag agctcaatca 1440

attaattctg caaacttcat gttcgttcaa gcagttcaag ttgctagaac tttgaagtca 1500

ccagattgtt tgggtatttt gttagaagaa ttggattgtt tgtttattgg tcaatcttgg 1560

gatttgtact ggaagttcca tactcaaatc ccaacagaac aagaatactt tgaaatggtt 1620

gattcaaaga ctggtgctat gttcagattg ttgacaagat tgatgttgca tacttcatct 1680

gttactgcaa cagatgattc tgctcaattg ttagaagcaa tgtgtagatt gttaggtaga 1740

tttttccaag ttagagatga tttcatgaat ttgtcatcta acgaatattc agatttgaag 1800

ggtttctgtg aagatttgga tgaaggtaaa atgtcttacc caatggttat ggttttgaga 1860

caaaacccag aataccaaga tcaaatcatg ggtacattga gacaacaagc tatgtctgca 1920

gctaaaggtg gtttatcaca accagttaga ttgtctagag aaactaaggt ttacatcttg 1980

ggtttgttga aatcatctgg tgcaatggct actacattga agaaattgca agaattggaa 2040

acagctttag aatctttgat tgatggtttg gaaactggtt ttggtgaaac aaatccagtt 2100

atgagaattt tgttatcaag attatctgtt agagatatta ctttg 2145

<210> 15

<211> 2142

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

atggctgaat caatgtggaa atattctact gcattagatc cagaaatggt tagaagaaca 60

ggttgtttca ctacattgcc agttagaatt aataacagag atgatatccc aaatgctgca 120

tcattgagag ttttaaaaga ttgggctgaa catacaggta ataaggatat ctctcaagat 180

agattatctt tgtcaccagt tggttctttt tgttcattga tctattgtga aactatccca 240

gaaagattgg attctatctc atacttgact gatttgtttt tcttgatcga tgatgctaca 300

gaagaagttg caaatgataa agctgcacaa gaagaatggg ctggtttttc aggtgcaatg 360

actgattctt taggtgaaac accacaaaga gatcatgatt tggagtttat gaagaaaaag 420

aaattgactg ctcaagttat gttggatttc atgacaatcg atgcagaatt gggtttggat 480

ttggttaagt catgtaaagc tggttggact ccattagctg caggtgttga atggccaaca 540

atcgaagatt atttggtttt tagaagagat tctgctggtt tggatatcta ttggggtaaa 600

actgttttcg gtttgggtga aaaattgaca gatgatgaag aaaaattgat cagaccattg 660

gtttgggctg cagaaaaggc tgcaatgttg aacaacgatt actggtcatg ggatgttgaa 720

tactctgaag ctaatcaaaa gactgaacat ttgacaaatg ctgttgcagt tttgatgaag 780

aacgaaggta tctctgcaga agagggtaaa gaaagaatta aaggtttgat catcgattat 840

gaagctgaat acttgagatt gagagcacaa ttctacgaaa accatccatc agctagattg 900

tacttaagaa agagagttga attggcaggt tctatggctg caggtgtttc tttttggtca 960

gctaattctc caagatacca tttgtctaag aaacaagttg aagctgcagg tttgccagat 1020

gatccaagaa caaaaccaaa tcaaacttca gatacaggtt ctccaatggg tagatcttca 1080

actgattcac aagttgcttc tgatatcaca tggtctactt caacagatat ttcttcacaa 1140

tcacaacata cacaagattt ggttgcacat tcttattacg gtgttccaaa attggataga 1200

actgctttga atggtccaat tgaatacgtt tcagcaatgc catctaaagg tgttagatct 1260

tcattgattg atgctatgaa tcaatggtgt caagttccat cttcacaatt ggcagttgtt 1320

aagtcagtta ttgatcaatt gcataattct tcattggttt tagatgatat ccaagatgat 1380

tctccaatga gaagaggtaa aactgctaca catttgattt ttggtcaagc tcaagcaatt 1440

aattcagcaa atttcttgta cgttagagtt gttcaagaag ttcatgctac tagaaataca 1500

gcattgatgg atatcttgtt agaagaatta gaagatttgc atgttggtca atcttgggat 1560

ttgtactgga agtacaattt gagatggcca tcagaagctg aatacttttc tatgatcgat 1620

ttgaagactg gtggtttgtt tagaatgttg gttagaatga tgcaaacatt gtctccattg 1680

aaggatagag atttcgtttg tgatactttg gtttcaatgg tttctagatt tttccaagtt 1740

agagatgatt atttgaattt gaatagcaga gaatactcaa ctcaaaaagg ttggtgtgaa 1800

gatttggatg agggtaaatt ttcatatttg atcatccatt gtttgcaaca ttctccaaag 1860

tttagagata gaatcatggg tttctttaga caaagaactg gttgtgttgg tccaatgcca 1920

acagttggta aagttcaaat catcgaatac ttacaagaag ctggttcttt taatgcatgt 1980

tgggaattgt taaattcttt ggaagatgat attgaaaagg aaattaaaag attagaagaa 2040

atcacaggtg aaaagaatcc attgatgcat ttgttgttga aattgttgtc tgttaagact 2100

gaaaagccaa acggtaaagc tgttgttgct ccagcaggtt ta 2142

<210> 16

<211> 2184

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 16

atggcagaat ttgctattcc agttccagat gatgttgtta aacaatctgg tacattgtca 60

agatttccaa ctgcagttca tagagaacat gctagatgtt tagctgcagc taataagatt 120

agagatgatt ttgcagctca agttgattgg gatttggatg ctaaaactac aggtcattat 180

ccaacattag gtgcagttca tgttgttgct tttactatgc cagaatgttt gccagaaaga 240

ttggcattaa tgactagatt cactgatttc actatcatga acgatgatta ctacgatgct 300

gttgatagag atcaagcaac atcttttaat gctgaattgc aaagatcttt aggtagagat 360

tgtcattcaa atacagttca gggtaatgca tctgttgcta ttaaaactaa gcaattccaa 420

gcatctatct tggttgaaat gatggttatg gatagagatt tggcaatgga tgttatggat 480

acatactctg atggtttaga aactgctaca tttccaccat cagatatttg tactatcgaa 540

gaatacttgc cagttagatt ggttaactgt ggtttggatg ttttccaaga aatgtcttgt 600

ttcggtttgg gtgttcattt gacaaaggca gaaaaggaaa aattgtcaga tatcgcaaac 660

acagctttgt atactgcagc tttgattaat gattgtcatt cttggccaaa agaattgaaa 720

catcatttgg aaactccagg ttcagatgtt ccttttaatg ctgtttgtat cttgatgaga 780

caattcaatt gttctgatgt taaggcaatc gaaagattga gagctatcta tgttgaaatc 840

caagaaagac atttgtcatt agttagaaat ttggaacaat ctgaaggttc aatcccagaa 900

acacatagaa agtacatcat ggcagctcaa tacgcagctt ctggttcaga attttggtct 960

ttgtatgctc caagataccc atcaaaagaa gatttggaac aaccagaata cgttttggtt 1020

gataacgttt tgcatagaag atctatgtca gataaggatt tgccaacttc tgataaggat 1080

ttggcaagag ctgattcagc aatgcatatc gaaacaatta aaactgctgg ttcttcaggc 1140

atgtctcaca tgaatgaagc atactcttca acaccagcta ctgaaatggt tgcatgggat 1200

gctggttcag aaatcatcca tacagaaatc gattctaatg gttcaaaaga attggcacca 1260

aatggtgctc aaactagagt tcaaaaacca tctgaagatg cagttagagc tccatacgat 1320

tacatcagag cattgccatc taagagaatt agagaaactt ttattgatgc tttggattca 1380

tggttagcag ttccagctgg ttcttcagca tctattaaat caatcatcgg catgttgcat 1440

caatcttcat tgatgttgga tgatatcgaa gatgattcta cattgagaag aggtaaacca 1500

acagctcata ctttgttcgg tatcgcacaa actattaatt ctgctaactg ggttttcgca 1560

tgtgctttcg aagaattgag atcattgaga ggtgttgatg cagctactat cttcgttgaa 1620

gaagttaaaa atttgcattg tggtcaagca ttggatttgc attggaagca tcatacatac 1680

atcccatctg ttgatgaata cttgaacatg gttgatcata agactggtgg tttgtttaga 1740

ttgtgtgtta gattgatgca gggtgaatct tcaacatctt gtcatcatat cgatgctgaa 1800

agattcatta ctttgttggg tagatacttc caaatcagag atgattacca aaatttggtt 1860

tcagatgaat acacaaacca aaagggtttc tgtgaagatt tggatgaagg taaaatttct 1920

ttgccattga tctattgttt agcaggttca gatccaactc aaatcatgat cagaggtatt 1980

ttgcaacata aaagagctgg tgaaatgcca ttgtctatga agaaattgat cttggaaaag 2040

atgagatctg gtggtgcatt gaatgctact atttcattgt tgaaggattt gcaagataac 2100

atcttagaag aattgaaatc tttagaatca gctttcggtt ctggtaatcc aatgttggaa 2160

ttagttttga gaagattatg gatt 2184

<210> 17

<211> 2052

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 17

atgtctccag ttgatatttc ttttattcat tcagaattag ttgatagaga agaagttgca 60

agagtttgtg ctactacatt gccagttaga aagtctaaat attcaccatt agttgaaaag 120

gctgttttgg aatttcaaca acaatggcaa gaagaagttg gttttgcata ctgtggtgct 180

acttctccac aaggtccagt tacagttttc tttccaccag aaactaaaca agatagagtt 240

gaaatcttcg caaaattgat cgaatatttc tttgcttacg atgatgtttt gacagcacca 300

ggttctgcta aaactggtga agaacaatct tcagattcaa ttggttggga agttagaaaa 360

ggtactacat ctagagttag aatctcagct atgaagcaaa tccaatctga agttttcttg 420

agattgttag aaatcgatag aaagcgtggt aatttgatct tgagagctat taatgatttg 480

tctagagttc atggtacaat tggttctaga gatttgagaa cttgggatga tttggttcaa 540

tatagagcag atgattacgg tgctgaattg aacatcatgt caatcatcta ttgttgtgaa 600

ttagatttga cacaaggtga cattcaagca ttgaaagaaa tttggtctcc agctactgct 660

gcagctgcat tggttaacga tttgtactct tttaatagag aagttatttt agaaccagat 720

actgatacag atactacaat cactacacca aattctgttt ggtacttaat gaagacattg 780

aatttgtctg tttcacaagc aaaggaattt ttggttaagg ataagatctt accattggaa 840

agagagttta ttgctaagaa agctgaatac ttagctaaca tgaatccatt gactccaaag 900

tctggtgaca tcatatactt tttggaaatg gttggtttgg gtttgtctgg taattggtac 960

tggcatgcaa ttgctgatag atttcataga tgggcagaat tgttacaatt accaccagct 1020

aaattgtttg attatgatga agcaactgct acatgtgcaa catttttgaa cacacaatct 1080

ttgagaggta gaaagaatag aattttagaa tctacagaat cattgcaagc tatgactgat 1140

gatccatact acaaagtttt acatcaacca atcgattact tgagatccgt tccatctaaa 1200

aatatcagag gtacaatcat ccaagcatta aatttgtggt tgaatgctcc agaatctgct 1260

gcagctcaag ttgaagattt gattggtcat ttgcatgaat cttcattgtt gttggatgat 1320

atccaagatt cttcagaatt gagaagaggt agaccatcag cttatagagt ttttggtgtt 1380

ccacaaacta ttaatgcagc tactcatgca ttgacattgg ctttcgaaaa ggttgttcca 1440

ttgatgaagc cagattcttc acatgttttc tttgatgaat tgagaaattt gcatgttggt 1500

caagcaatgg atttgtattg gacaagatct ggttacagac catcaatcgc tgaatatttg 1560

gaaatgaaca gattgaagac tggtgcatta ttttgtttgg cttctaattt gttgtcaaac 1620

caaggttctt tttcagcagg tgctattaaa caaacagatt tgaacgattt gatgatttct 1680

ttaggtcaat attttcaagc aagagatgat tacatcaatt tggcttcaac taagtaccaa 1740

gaacaaaagg gttttgctca agatttggat gagggtaaat tgtctttacc attgatccat 1800

ttgttgacac aatcaccaaa cgcagctttg atcgaaaaca tccaacaaga aagagcaaga 1860

aacaataagt tgccagctga tttgaagcaa ttgatcttgg atgaaatgag agatcaaaag 1920

attatgcaat tgactgaaga aacattgaac ggtttggaag caaaggttta cagatactta 1980

gaaagattgg aagtttctgc tggtattaaa aattttactt ttagattttt attggataga 2040

ttgagagaaa tg 2052

<210> 18

<211> 2097

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 18

atggatttgc caacttctga attggatgct acttggacaa attctaagtc agttgatttg 60

acaagagaaa gaccaccaga ttgtttcact actagagctg ttagaattaa taagtgtgat 120

gatgttatga atttgtacac tacaaaggca attaataact ggactgctgc aatgtgtgat 180

gatgctgata gaaaagttgg tcatgcattt tcttcagttg gtaacgtttc ttcttttatt 240

ttcccagaat gttcaccaga taaggctcat atcttggcat acttcactca agtttctttt 300

attcatgatg atgcattgtc ttcaaaggat tcaaaggaag aaaataagca tttgtctcat 360

gctttggatc caaaggataa caacccaggt acatcagaac gtggtaaagc aatgaagaaa 420

ttcttgtctc aaactgtttt ggaattgatc gatatggata caaacgaagg tcaagaattt 480

gttaaaaatt tgaaagtttg ggctgatgat gaaatcggtt tgaaaaatcc acaaacaatc 540

gaatgtgttg atgaatattt gcatttcaga agattaaatg gtggtattaa agcatattgg 600

cattggttag cattttctca tgaagataga ttcactcaag ctgattggga ttcaatcgaa 660

gatttgttga agtctgcaaa tagagttttt attttgacaa acgattactt ctcatggcca 720

agagaaagat tgtacggtca aggtagaatc gctaacgtta tcgaatttta catgagaact 780

gaaggtttgt ctgaagaaga agcaaagcaa agaacaaagg aagaaatctt gcaaggtgaa 840

catttgttcc ataacatgtg tgttgaaaga ttcgctagag aaccaaattt gccaagacat 900

gttaagaaat tgttacaagt tgctgaagtt gcaatgggtg gttataatta ctgggcttca 960

acttgtccaa gattaaattc ttggaaagaa caagctccaa ctgcagaaac agatttccat 1020

ggttctaaaa atgatgaagt ttctaaccca actggtaaag ctgaattggt taagccagtt 1080

aagactaacc aaacatcagg taaaatccaa gcaatcacaa aggttcaatc tttaccatct 1140

gtttcacatt tcacttgtac atcagatttg gatgattctg ctttgttagc tccagcacat 1200

tatgttgaat ctttgtcttc taaaaatgtt ttgtctaaat tggttgaagc ctttaatgtt 1260

tggatgcaag ttccaccaaa accattggct gcaattaaac atgttttgga tgatttgcat 1320

aattcttcat tgatcttgga tgatatccaa gataattctc cattaagaag aggtagaact 1380

gctacacatt tgatttttgg tccagctcaa tctattaatt cagcaactta catgttcgtt 1440

aaagctgcac aaacagttga tgctttaggt actccacaaa tgatgacagc atttttgcaa 1500

ggtttagaaa ctttgtttat tggtcaatct tgggatattt catggagaca atcttttcat 1560

tgtccaacag aatcagaata tttgtctgct gcagataaga aaactggtgc tttgttgaca 1620

atgatggttg aattgatgca atgtaacgca aagactttgc cattttcata cagattgtct 1680

ccattggcta gattatttgg tagatggtac caagttagag atgattacat gaatttgcaa 1740

ggtgctgatt actctaagca aaagggtttc tgtgaagatt tggatgaggg taaattatca 1800

tacccaatct tgaagtgttg tcaaaaatct gaaactaata agggtatcat cttgggtatt 1860

tttagacaaa tgagaatgac taacacaaag atgatgagag aatcaaaatt gcatatcttg 1920

gatttgatgt cttcagctag agcattggaa gatacattcg attatttgca acaattgcaa 1980

caagaaatcg aaagagatat cagagaaatt gaagttttag ctggtgaatc taacccagaa 2040

ttgttgttgt tggttaaagt tttgggtgca atcccaaaac caggtaaaaa gggtcat 2097

<210> 19

<211> 2238

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 19

atggatatgt tggatggtca tacttctgaa ttgatcggtt tgtcaagata cgatacacat 60

ggtttcttgt ctccaggtgg ttacactttg agaagacata aggcagaaac attggcaaat 120

gctggttgtt atgaagctag acaagattgg attcaataca ttggtgctgc agctgaagaa 180

tttggtaact gtaacccagt taacggtaac ttcactgcag ttgttttacc attggctaaa 240

ccagaaagat tgagattggt tgcatacatc ttggaatacg catttttgca tgataacatc 300

gttgaagttg caaaggataa cgcttcttca tctaaggata acgatgcttt ttctttgggt 360

gacatggaaa catcacatca aaacgcaaaa ttgggtagaa agcaaatcca agctaagatg 420

atcttacaat tgactcaaac agatccagtt tgtgctaaaa gagttatggg tgtttggcaa 480

caaatgttgg aaactacaat taaagacaag tcaaaatctt ttgcaaattt ggaagaatat 540

ttggagttta gaatcatcga tactggtgct ccattcgttg aatctgttat gttgttcggt 600

ttgggtgaaa ctttgacaaa ggaagaagat tcattgttgg caccaatcat cagaccatgt 660

tatgcttctt tagcattggc taacgattac ttctcattcc atagagaatg ggcagaatac 720

caatctgctc aatcatcttc atcttcatct ccagatgctc catcagcagc tccagcagct 780

ccaattaatt tggtttattt gtacactcaa tggcaatctg ttgatattcc aacagcaaaa 840

agattggtta gagaagcagc taacagattc gaagctgatt tcttgagaaa gtgtgaagat 900

ttcaagaaat ctggtcaatc taacgataaa ttggatagat atttgagagg tttgcaatac 960

caagtttctg gtaacgttgt ttggtcattg aactgtccaa gatatcatcc agaatggaga 1020

tacgatccaa ataagggttt ggaagatgca ttaactgctg aaagaagaaa tccaccattt 1080

gttgaacaag ttgaagatgt tgatgcatca gtttctgata aagaagcagc tacaaaaaga 1140

atgtcaattg cttctggtgg ttcaagagaa aacgattctg atgtttcaac tacatcatct 1200

tcatgggatg atagagcagc ttctagatct tcatcttttt cagcaatgtt ggatgatgat 1260

gaatctggta tgagagagtt taaagaagct caaagagaat tatctttgga ttcattgtta 1320

ccatctgaag aaaaattggg tatggaaatc gttaacgctc cattcgaata cactagattg 1380

atgccatcta aaaatgttag aggtactttt attgatgcat taaatttgtg ggctggtttg 1440

tctgaagaag ttttaggtca aattaaagaa gttgttgatg atttgcatac tgcatcttta 1500

atgtttgatg atgttgaaga tggttcagaa ttgagaagag gtaatccagc agctcatgct 1560

gtttacggta ttccacaaac aattaattca gcatctttag ctatcttgga agcagttcaa 1620

aaagctaaag atttgccaat tccaggtgca gttgatatcg ctttagaaca attgagagat 1680

ttgcatgttg gtcaatctta tgatttgtac tggactagac acatgacatg tccatcagaa 1740

tctgaatact tagaaatggt tgctaagaaa actggtggtt tgtttttgtt gttgtctaga 1800

ttaatgtcag aacacatgcc aaaggaagtt agatctttgg ttaacgattt ggttactcaa 1860

gttggtatct atttccaaat cagagatgat taccaaaatt tgtcatctga tgaatacaca 1920

gctcaaaaag gtttctgtga agatttggat gagggtaaat tgtcttttcc attggttcat 1980

tacttgaaca cagaaagagg ttcatctaat tcacaacaag ttagagaagt tttacaagaa 2040

agacaattga gaggttcatt gtctatgcca ttgaaattgt tgactttgca aagattgaaa 2100

tcatctaatt ctttggaata cacaagagat tcattgatga gattagaaag aggtgttgat 2160

ggtactatcg aagaattaga aagatctaca ggtaaaaaga attgggtttt gagaatgtgt 2220

atggctaaat tgtcagtt 2238

<210> 20

<211> 2271

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 20

atggcatctg aaatgtggaa atattcttca ccaattgatc cagaagttgt taaagctaca 60

ggttgtttca ctacattgcc agttagaatt aataatagag atgatttggc taatggtgca 120

tcttcaagag ttttgaaaga ttgggctgaa catactggta accaatctat cgatagaaac 180

agagtttctt tttcaccagt tggttctttt tgttcattga tctattgtga aacaatccca 240

gaaagattgg attctatctc atacttgact gatttgtttt tcttgatcga tgatgctaca 300

gaagaagttg aaaacgatac tttggctcaa gaacaatggg caggtttttc aggtgctatg 360

acagattctt tggcagaagc tgcaccacaa agagatcatg atttggatat gatgaagaaa 420

aagaaattgg ttgctagagt tatgttggat ttcatgagat tggatccagt tttaggtttg 480

gatttggtta agtcatgtaa agcaggttgg acaccattaa ctgctggtgt tgaatgggaa 540

actatggaag attatttgag attcagaaga ttgtctgctg gtttggatat ctattggaca 600

aagactgttt tcggtttggg tgaaaaattg acagatgatg aagaaaaatt gatcagacca 660

ttgacttggg ctgcagaaaa ggctgcaatg ttgaacaacg attactggtc ttgggatatc 720

gaatacttac aagcaggtgg taacatcgaa aaattgacaa acgctgttgc agttttaatg 780

agaaaagaag gtttgactgc tgaagaaggt aaacaaagaa ttaaaaattt gatcatcggt 840

tacgaagaag aatactcaag attgagagat gatttctaca acgctcatcc atctgcaaga 900

ttgtacttaa gaaagagagt tgaattggca ggttcaatgg ctgcaggtgt ttctttttgg 960

tcagctaatt ctccaagata tcatattcca acacaacaaa ctgaaactac aactgaatct 1020

ggtgaagctg cacaaccagc tgcaggtaca tggcatgctg aagttagaga tacttcaaga 1080

ccaggttctg attcttcagt ttcttcaaca tctgaatcag atgattcaat tgatgcttct 1140

gcatcttcag gtacaacaac tactacaact acaactacaa ctgctttgtc ttcatctcaa 1200

acttcattat catcttcatt gtctgaagca gaagaagaag aagaagctca taaaccacca 1260

ccatgttttg atgctccagc aaaattgggt agagctgcaa ttgatgcacc aattgattac 1320

gtttctggta tgccatctaa aggtgttaga acttcattga ttgatgctat gaatcaatgg 1380

tgtagagttc catctgctca attaggtgca gttaagagag ttgttgatgt tttgcataat 1440

tcttcattga tcttggatga tatccaagat gattctccaa tgagaagagg taaaacagct 1500

actcatttgg tttttggtgc tgcacaagct attaattcag caacattttt gcatgttaga 1560

gcagttagag aagttcatgc tactggttct gctgcattga tggctgtttt gttagaagaa 1620

ttagaagatt tgcatgttgg tcaatcatgg gatttgtact ggaagtacaa tttgagatgg 1680

ccaacagaag atgaatattt ttctatgatc gatttgaaga ctggtggttt gtttagaatg 1740

ttggttagaa tgatgagagt tttggctcca gaaccaacag gtggtgaaac taaaggtggt 1800

gaatttgctt gtgatgcatt ggtttcaatg gtttctagat ttttccaagt tagagatgat 1860

tatttgaatt tgaactctag agaatacggt tctcaaaaag gttggtgtga agatttggat 1920

gagggtaaat tttcatactt agttattcat tgtttggaaa catctccaag attcagagat 1980

agagttatgg gtttgtttag acaaagagca ggtacagctt cagcatcttc aggtccaact 2040

ccaatgccat ctgttgctaa ggttcaaatc atcgaatatt tgtacgaagc aggttctttt 2100

gatgcttgtt ggagattgtt agttagatta gaagatgata tcgaaggtga aatcagaaga 2160

ttggaagaag caactggtga agaaaaccca caaatgcatt tgttgttgaa attgttgtct 2220

gttaaaaatg ataagccaaa taagggtcca gttgttgttc cagctggttt g 2271

<210> 21

<211> 2154

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 21

atggctggta ctagatcttc aagaccaggt gcaacttctt ttattcaaca ttctatccca 60

ttaccaagat ctgcttatga aggtgttgaa tacttttgta gattcagacc aagaatacat 120

agagatgcaa ttttggctga tgcaggttca tggcaatgtc aagttgattt ctttggttct 180

tcagctacag caagagctga ttcaatcaga aataagaacc atacttctta tgctgttggt 240

tgtatcaacc cagttgttgg taacttcaca gcattatgtg cttgtgaagc aattccagat 300

agattggctt tgactacata catggttgaa tacgcataca tccatgatga tgttatcgaa 360

tacgctgaaa ataaggatga agatagagat aacgttagaa gaagacaatt gcaagctaaa 420

atggcagttg aattgatgga tatcgataag gttaagggta aagaatgttt gagattgtgg 480

aaggaaatgt ctgatgtttt cgttcaaatc agagaattga agtttactaa attggatgat 540

tatttgactt ttagagttat tgatgctggt tgtccatgga caatgtcatt gttgtgtttc 600

tctatggatt tcactttgaa cgatgatgaa gttgaaaaga ctgctgcaat tacatcagct 660

gcttatgatg gttgggtttt ggttaacgat tacttctctt gggaaaagga atggaagaac 720

catcaagcta acggtggttc aggtgttatt gcaaacgcta ttttcttgtt tatgagatgg 780

tactctgttg atgcagttga aggtagaaga atgttgagaa aggaaatctt ggctagagaa 840

gaaaaatact gtaaggcaaa ggaagaattt ttagtttcag gcaacgttac tgataagaca 900

tctcaatggt tggaattgtt agatcatgtt actgctggta attttgcatg gtcaatgact 960

acagctagat atcaattagg tggtaaagat gcttacccag cattgagagc tgcaaatact 1020

gataattggg aaacatcaac tacagattca ttgtctaacc caatctctca taacgctgat 1080

aagatcgcaa gaaaaattaa tttgattttt aaggaacaaa agttcttgga tgctagaggt 1140

ttggttaatc atactgaaga ttatccacca attgttttga cagcacaagt ttctcaacca 1200

gatgaaactc cagagtttat tcattcacaa gttactcaag ctagatcttt tacacaatac 1260

gaaaagatga tcttgcaacc acaaaactac ttggaatcaa tgccatctaa gggtgttaga 1320

aattctgtta ttgatggttt agaaatgtgg taccaagttc cagaaagatc attggctaca 1380

atcagaaaga tcgttaattt gttgcattct tcatctttga tgttggatga tatcgaagat 1440

aactctccat taagaagagg tttgccagca actcatacag tttttggtat ttcacaaact 1500

attaattctg ctaatttgtt gatgttcaaa gcattaaagg ctgcagaatc attgtctcca 1560

gctgcagtta gaattttcat tgaaagaatc atcgaaggtc atatcggtca gggtatggaa 1620

ttgtactgga ctttccatac agaaatccct acagaagaag aatactttgt tatggttgat 1680

ggtaaaactg gtggtttgtt tattttgttg gctgaattga tgagatcaga agcaactaga 1740

cataaggatt tggatacatc tttgttgatg aaattggttg gtagattttt ccaagctaga 1800

gatgattacc aaaatttgga atctgctcaa tacacacaac aaaaaggttt tgcagaagat 1860

attggtgagg gtaaattgtc attaccattg atccatgctt tgggttctaa aacaccacaa 1920

agaggtagat taatgtcaat cttgcaacaa agaaaatcta ctgttgattt gccattccat 1980

atcagaaaat tggctttgga tgatatcaaa gcaactggtg gtttgaagta cgctaagaaa 2040

atggcaatgt cattgcaaga ttctgttaac gaaactttga cacaatacga agataaggtt 2100

ggtgctaaaa attggatctt gagattggtt caaaagagat tagaattgga agtt 2154

<210> 22

<211> 2136

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 22

atggctgaag aaatgtggaa atattctatc ccaatcgatc cagaaactgt taagcaaaca 60

ggttgtttca ctacattgcc agttagaatt aataacagag atgatttggc aaatgctgca 120

tcttcaagag ttttgaaaga ttgggctgaa catactggta accaaaacat cgatccaaac 180

agagtttctt tttcaccagt tggttctttt tgttcattga tctattgtga aacaatccca 240

gaaagattgg attctatctc atacttgact gatttgtttt tcttgatcga tgatgcaaca 300

gaagaagttg aaaacgataa ggttgctcaa gaacaatggg caggtttttc aggtgctatg 360

actgattctt taggtgaaac accacaaaga gatcatgatt tggattctat gaagaaaaag 420

aaattggttg ctagagttat gttggatttc atgagattgg attcagcatt gggtttggat 480

ttggttaagt cttgtaaagc tggttggact ccattggctg caggtgttga atgggaaaca 540

atggaagatt atttgcattt cagaagatta tcagctggtt tggatatcta ttggactaag 600

acagttttcg gtttgggtga aaaattgtcc gatgatgaag aaaaattgat cagaccattg 660

gtttgggctg cagaaaaggc tgcaatgttg aacaacgatt actggtcttg ggatatcgaa 720

tactttcaag caaaccaaaa gattgatatg ttaactaatg ctgttgcagt tttgatgaga 780

aaagaaggta ttacagctga agaaggtaaa aatagaatta aaaatttgat cttaggttat 840

gaagcagaat actctcaatt gagagctcaa ttctacgaat ctcatccatc agcaagattg 900

tacttaagaa agagagttga attggctggt tcaatggctg caggtgtttc tttttggtca 960

gctaattctc caagatatca tttgccaact gataaaccaa gaccatctgt tgttccagtt 1020

gaggttaagt tcgaagaaat gacattgaag tcttcagctg aatcagttgc atctactgaa 1080

tcagaaaatt ctatggctgc aacatctggt tcaggtactg aattaacatc acaatcttca 1140

cattctgatg gtttgagaaa gagatacttc gatgttccaa aattgggtcc agctgcaatt 1200

aatgaaccaa ttgattacgt ttctggtatg ccatctaaag gtgttagatc ttcattgatt 1260

gatgcaatga atcaatggtg tcaagttcca tcttcacaat tggctgttgt taagagagtt 1320

attgatttgt tgcataattc ttcattgatc ttggatgata tccaagatga ttctccaatg 1380

agaagaggta aaactgcaac acatttgatc ttcggtgaag cacaatcaat taattctgct 1440

acttatttgt acgttagagt tgttcaagaa gttcatgcta ctggtaatgc tgcattgatg 1500

aaagttttgt tagaagaatt ggatgatttg catgttggtc aatcatggga tttgtactgg 1560

aagtacaatt tgaaatggcc aactgagggt gaatattttt ctatgatcga tttgaagaca 1620

ggtggtttgt ttagaatgtt ggttagaatg atgcatgctt tgtgtcatcc agaatgtaaa 1680

gaagaatttg cttgtgatgc attagtttca atggtttcta gatttttcca agttagagat 1740

gattatttga atttgaatag cagagaatac tcaaaccaaa aaggttggtg tgaagatttg 1800

gatgagggta aattttcata tttgatcatc cattgtttgg aaaattctcc aagatacaga 1860

gatagaatca tgggtttgtt tagacaaaga actggttgtt caggtccaat gccatctgtt 1920

ggtaaagttc aaatcatcga atacttacaa gaagcaggtt catttgatgc ttgttgggaa 1980

ttgttgaata agttggaaga tgaaatcgaa gcagaaatca gaagattaga agctgttaca 2040

ggtgaagaaa acccacaaat gcatttgttg ttgaaattgt tgtctgttaa aaatgaaaag 2100

ccaaataagg gtccagttat tgttccatta ggtgtt 2136

<210> 23

<211> 2154

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 23

atgtgtacta tggatgatca tacatctgaa ttgttggata tctcacaatt cgatactcaa 60

ggtttctgtc caggttatcc attgagaaga catagatacg aatctttagc aaattcaggt 120

tgttatgaag ctagacaaga ttggattcat tacgttggtc cagctgaaga atttggttct 180

tgtgatccaa ctaacggtaa cttcacagca ttggttttac cattgactaa accagaaaga 240

ttgcatttgg ttgcttacat catcgagtgt gagtttatta gagttttgta cccatacgca 300

tttttgcatg ataatgttgt tgaagcagtt atgccagatt cttcaacaaa caacggtgac 360

gatttccatt tgggtgaagc tgaaaagaga caaagaaacg ttagaatggg tagaaagcaa 420

atccaagcaa agatgatgta ccaattggct agaactgata agagatgtgc agaaagagtt 480

aagagagttt ggaagacaat gttgtctact acattgaagc ataagtcaga ttctttcgca 540

tcattggaag aatatttgaa ctacagaatc atcgatactg gtggttactg ggttgaatct 600

gttatgttgt ttggtatggg tatgacttta acagaagaag aagatgctca attggcagaa 660

gttgttagac catgttatgc ttctttagct ttggcaaacg attacttctc attcgataga 720

gaatgggaag aagctcaaaa tggtggtcca ccaccaacaa atgcagtttg gttgtatatg 780

agatggaatg gtattccaat gccagctgct aagaaattgg ttagagaagc atctaacaga 840

tacgaagcta gatttttaga attgtgtgat tcttttagaa gaaacaaccc attgtactca 900

gaaaaattgg ataaatattt gagaggtttg gcttaccaaa tttctggtaa tgttgtttgg 960

tcattgactt gtccaagata tcatccagag tttagatacg atccaaatgc tggtatggaa 1020

gatttgttaa ctgctcaagc tagaggtgac ggtggtgctg tttctggtgg tgaagctgat 1080

tacacagcag aacatagaca atcaatcatt tctttagaat cacaacatac tgcttcttca 1140

acaagatatt cagcatctga ttggcaatct tcaagatctt catctttttc tgaaatctca 1200

gttgatggtg aagatggtga aactcatgca gttaaattgc cagctgaaca aggtttagat 1260

acaaaacatg ttgctgcacc atttgattat gttgcttctt tgccatcaaa gggtgttaga 1320

actacattga tcgatgcatt gaatttgtgg tgtgatttgc cagatactac attggctggt 1380

attaaagaag ttgttgataa attgcataca gcatctttaa tgttcgatga tatcgaagat 1440

ggttcagaat tgagaagagg taatccagct gcacatgcag tttacggtgt tccacaaact 1500

attaatgctg catcttttgc tattgttgat gcagtttcaa aagctcaaga tattccagtt 1560

acaggtgctg ttgatattgt tttagaacaa ttgagagatt tgcatatcgg tcaatcttat 1620

gatatccatt ggactagaca ttcatcttgt ccatcagaaa cagaatactt agaaatggtt 1680

gctaagaaaa ctggtggttt gtttaaatta atttctagat tgatgactac aggtttacca 1740

gatatcacta aatcaacaat cgatgatttg gttacacaat tgggtatcta tttccaaatc 1800

agagatgatt tcaagaattt gaactctgat gaatacgctt cacaaaaagg tttctgtgaa 1860

gatttggatg agggtaaatt ttctttccca ttggttcatt atttgactaa aggtcaagaa 1920

tctccacctt ttatgttgag agaaatcatg caacaaagaa gagaatctaa gggtttgaac 1980

gttccatcaa agatgttggc tttgcaacaa ttgaagaaat ctggttcttt agaatacact 2040

catagaacat tgaagagatt agaagttcaa attaatcaaa tcattggtag attggaaaga 2100

atcactggta aagataactg ggttttgaga ttgtgtatgg gtgaattaac agtt 2154

<210> 24

<211> 2109

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 24

atgatttcta ttgctccatt cccagatgaa gatcaatttt cagttttagt tccaagagat 60

actccagatt tgcatggttt ttgtcatggt tatccattaa gaagacataa agcagaacat 120

aaggctaacg aaggttcttt acaattgaga gcagattggg aaaaatacat tggtccaatt 180

gaaagatggg gttcatgtaa tccatgggaa ggtcattttg gtgctgttgt tttgccatgt 240

tgtagaccag aaagaatggc aatcgttaac tacatcttcg aatacgcttt tatgtacgat 300

aacgttgttg aatctgctgc acaatcaact attaatgctc atgcagatga tatcgcattg 360

gatgaaactg aatacagaac agttagatct gttactggta caaagcaagt tcaatcaaag 420

atgttgttag aattgtttgc tttggatcca gcttgtgcac aagttgtttt ggattcttgg 480

aagacaatga tcgatactac agcaactaag gataagacaa gagctttcca aaactgggaa 540

gaatacgttg attacagaat catcgatact ggtgctccat tcgttgatat gttgatgaga 600

ttcggtatgg gttttgtttt gacaccagaa gaacaagcat ctattgaacc agttgttaga 660

ccatgttatg ctgcattggg tttggctaac gattacttct cattcgatgt tgaatgggat 720

gaatttcaac atgcagaaga aactacaatg actaatgctg tttggttatg tatgcattgg 780

catggtgttg atgttccagc tgcaaaggaa atcgttagaa gagttacaaa caattttgaa 840

agagaatttc aacaaagagt tgcagaatat gttgctggtg acggtagaga aaacagaaag 900

gttcaagttt acttaagagc attgggttat caaattcctg gtaatgttgc ttggtcttta 960

agatgtccaa gataccatcc acatttggtt gaagaagctg gtagattgtt acaacaatct 1020

atgcaaactg catcagatga aggttgtgtt gctccagttc aagttcaagc aaaaagagct 1080

tctgtttcag aagaatctga atcttcagaa tcttcagttt ggtctggtcc aaattcacca 1140

agatcttcaa tttcttcagc atcagaagaa gaagaagcta gagaagttga agaagttaaa 1200

ttgggtgctg aacatttgtt gaacccagca gaatacatcg cttctttacc atcaaaaggt 1260

gttagagaag cctttattga tgctttgaac gtttggttga tgttgccaga tagacaagtt 1320

aaattgttga agtctatcgc aaagactttg cataacgctt cattaatgtt ggatgatatc 1380

gaagattctt caccattgag aagaggtcaa ccagctactc atacagttta tggtgttggt 1440

ccaacaatta attctgcaaa ctacttgttg ttgcaagcat tggatgaagt tagaggtttg 1500

gatgattcag atggtcaatg tttggatatc tatactgaag aaatgagaaa tttgtttaca 1560

ggtcaatctt atgatgttta ctggacaaga gaagcagttt gtccatcaga agctgaatac 1620

atggaaatga ttagacaaaa gactggtggt ttgtttagat tgttggctag attgatgatt 1680

gctacagcac cagctcaaag aaacagagat atcccattgt tgccattgat cgataaattg 1740

ggtgaatact ttcaaatcag agatgattac aagaatttga ctgaagaata cacagatcaa 1800

aaaggtttct gtgaagattt ggatgagggt aaattttctt tcccattgat ccatgcattg 1860

agagctcaag aaagagaaaa gaatggtaga ttatgggaaa tgttggaaca aggtagagca 1920

ggtggtacta tggaattggc tagaaagaga gaagttttag atatcttgca agattctggt 1980

tcaatggaat acactagaag aacattgcaa ggtattatgg gtgaaattca tggtggtatg 2040

ggtagagttg aaagagaaac aggtggtgtt aactgggttt tgagattgtt ggttatgaga 2100

ttggaagtt 2109

Claims

1. Use of a chimeric terpene synthase and/or nucleic acid molecule and/or construct and/or recombinant cell in the preparation of a terpenoid;

when the amino acid sequence of the chimeric terpene synthase is shown as SEQ ID NO. 1, the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 14; the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 14; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequence of the chimeric terpene synthase is shown in SEQ ID NO. 2, SEQ ID NO. 7 and SEQ ID NO. 9, the nucleotide sequence of the nucleic acid molecule is shown in SEQ ID NO. 15, SEQ ID NO. 20 and SEQ ID NO. 22; correspondingly, the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 15, SEQ ID NO. 20 and SEQ ID NO. 22; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequence of the chimeric terpene synthase is shown as SEQ ID NO. 3, the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 16; the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO:16; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequences of the chimeric terpene synthases are shown as SEQ ID NO. 5 and SEQ ID NO. 11, the nucleotide sequences of the nucleic acid molecules are shown as SEQ ID NO. 18 and SEQ ID NO. 24 correspondingly; correspondingly, the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 18 and SEQ ID NO. 24; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequence of the chimeric terpene synthase is shown as SEQ ID NO. 6, the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 19; the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 19; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequence of the chimeric terpene synthase is shown as SEQ ID NO. 4, the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 17; the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO:17; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

when the amino acid sequence of the chimeric terpene synthase is shown as SEQ ID NO. 10, the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 23; the nucleic acid molecule encodes the chimeric terpene synthase, and the nucleotide sequence of the nucleic acid molecule is shown as SEQ ID NO. 23; the construct contains the nucleic acid molecule; the recombinant cell comprises the nucleic acid molecule; the structure of the terpenoid is as follows,

2. a preparation method of a terpenoid is characterized by comprising the following steps: culturing the recombinant cells to obtain a culture product; and isolating the terpenoid from the culture product, the recombinant cells comprising a nucleic acid molecule, the terpenoid having the structure shown below,

when the terpenoid is a compound shown as a formula 5, the nucleotide sequence of the nucleic acid molecule is SEQ ID NO. 14 and/or SEQ ID NO. 17;

when the terpenoid is the compound shown as the formula 2, the nucleotide sequence of the nucleic acid molecule is at least one of SEQ ID NO. 15, SEQ ID NO. 20 and SEQ ID NO. 22;

when the terpenoid is the compound shown as the formula 1, the nucleotide sequence of the nucleic acid molecule is SEQ ID NO 16;

when the terpenoid is the compound shown as the formula 6, the nucleotide sequence of the nucleic acid molecule is at least one of SEQ ID NO. 18, SEQ ID NO. 23 and SEQ ID NO. 24;

when the terpenoid is a compound shown as a formula 24, the nucleotide sequence of the nucleic acid molecule is SEQ ID NO. 19 and/or SEQ ID NO. 23;

when the terpenoid is the compound shown as the formula 23, the nucleotide sequence of the nucleic acid molecule is SEQ ID NO. 17.

3. A terpenoid is characterized in that the structure of the terpenoid is shown as a structural formula 1 or a structural formula 2,