CN113466311A - Seawater measurement method based on seawater in-situ culture microbial film - Google Patents
Seawater measurement method based on seawater in-situ culture microbial film Download PDFInfo
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- 238000009629 microbiological culture Methods 0.000 claims abstract description 6
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Abstract
The invention discloses a seawater measurement method based on seawater in-situ culture microbial film, which comprises the following steps: carrying out microbial culture on the aerated seawater sample to obtain a microbial film; measuring the endogenous respiration value of the microbial membrane; measuring the microbial film exogenous respiration value of a water sample to be measured; and measuring the biochemical oxygen demand of the water sample to be measured. The seawater measurement method based on seawater in-situ culture microbial membrane achieves the technical effect of simple and reliable measurement.
Description
Technical Field
The invention relates to the field of water body pollution measurement, in particular to a seawater measurement method based on seawater in-situ culture microbial film.
Background
At present, water pollution is a worldwide problem, domestic industrial wastewater contains various organic matters and inorganic matters and is discharged into the sea without being properly treated, so that negative effects are brought to marine organisms and aquatic resources, and the water body environment is damaged. Biochemical oxygen demand BOD, one of the important indicators for measuring the degree of water pollution, means that under the specified conditions, some substances which can be oxidized in water are decomposed by microorganisms, especially the substances which can be oxidized are generated by decomposing organic substancesThe dissolved oxygen consumed in the biochemical process, generally 5 days as the standard time for determining the biochemical oxygen demand, is called the five-day biochemical oxygen demand BOD5The higher the biochemical oxygen demand value, the more serious the organic pollutants in the water body. Conventional BOD5The measuring method has long measuring period, many interference factors, poor repeatability and larger error, and can not reflect the change condition of the seawater quality in time. To solve this problem, there are many new types of BOD detection5The method of (1), wherein BOD is measured using a microbial sensor5The method is widely applied, but because the selection conditions for the types of microorganisms are harsh, the broad spectrum for degrading organic matters is poor, the types and the quantity of fixed microorganisms can be influenced by the microorganisms in a water sample, the measurement result is unstable, and the measurement effect is not ideal.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the seawater measuring method based on the seawater in-situ culture microbial membrane can finish the detection of the biochemical oxygen demand of the water sample to be detected only by adopting a single oxygen electrode, has simple and effective detection process and reduces the preparation and use cost of equipment. The water samples containing the microorganisms adopt seawater in different regions, the microorganisms cultured in situ in the seawater have strong environmental adaptability and are not influenced by seawater with high salt content and artificial seawater, meanwhile, the seawater samples to be detected are used as the background to prepare the standard solution, compared with the method for preparing the standard solution by using a phosphate buffer solution, the detection effect is more accurate, the error in detection is reduced, the preparation of the buffer solution and the secondary pollution to the environment are avoided, the seawater samples containing the microorganisms are subjected to microorganism culture in a reactor, and a microbial film is formed on the inner wall of the reactor, so that the microbial film reactor is obtained. The method for detecting the biochemical oxygen demand of the seawater can be finished in about 20 minutes, and is similar to the traditional BOD5Compared with the method, the detection speed is greatly improved, and in the detection process, microorganisms in the seawater water sample can replace inactivated microorganisms in the microbial membrane to complete the self-repair of the microbial membrane. The stability and the service life of the microbial film are greatly improvedHigh, the microbial membrane is preserved for 1 week, and then the re-culture is almost the same as the previous signal response value. Compared with the method for fixing one or more microbial sensors, the method has the advantages that the degradation efficiency is enhanced, and the non-selective degradation is realized in the face of different seawater samples. The seawater measurement method based on the seawater in-situ culture microbial membrane achieves the technical effect of simple, convenient and reliable measurement.
The invention provides a seawater measuring method based on seawater in-situ culture microbial film with the above functions. The method comprises the following steps: carrying out microbial culture on the aerated seawater sample to obtain a microbial film; measuring the microbial membrane endogenous respiration values; measuring the microbial film exogenous respiration value of a water sample to be measured; and measuring the biochemical oxygen demand of the water sample to be measured. The seawater measurement method based on the seawater in-situ culture microbial membrane achieves the technical effect of simple, convenient and reliable measurement. The biochemical oxygen demand of the water sample to be detected can be detected only by adopting the oxygen electrode, the detection process is simple and effective, and the preparation and use cost of the equipment is reduced. The water sample containing the microorganisms adopts seawater in different regions, the microorganisms cultured in situ in the seawater have strong environmental adaptability and are not influenced by seawater with high salt content and artificial seawater, and meanwhile, the seawater sample to be detected is used as a background to prepare a standard solution, so that compared with the method of preparing a phosphate buffer solution, the detection effect is more accurate, the error in detection is reduced, and the preparation of the buffer solution and secondary pollution to the environment are avoided.
According to the seawater measurement method for the in-situ culture of the microbial membrane based on seawater, disclosed by the invention, the measurement of the endogenous respiration value of the microbial membrane comprises the following steps: enabling the seawater with the preset BOD value to flow through an oxygen electrode to obtain the initial oxygen content of the seawater; the seawater with a preset BOD value flows through the microbial membrane and the oxygen electrode to obtain the oxygen content of the microbial membrane after endogenous respiration; and subtracting the oxygen content after the microbial membrane endogenous respiration from the initial oxygen content to obtain the microbial membrane endogenous respiration value.
According to the seawater measuring method based on seawater in-situ culture microbial membrane, the method for measuring the microbial membrane exogenous respiration value of the water sample to be measured comprises the following steps: the water sample to be detected passes through the microbial film and the surface of the oxygen electrode in sequence to obtain the oxygen content after oxygen consumption; directly passing a water sample to be detected through the oxygen electrode to obtain the initial oxygen content of the water sample; and subtracting the initial oxygen content from the oxygen content after oxygen consumption to obtain the microbial film exogenous respiration value of the water sample to be detected.
According to the seawater measuring method based on seawater in-situ culture microbial membrane, the method for measuring the biochemical oxygen demand of the water sample to be measured comprises the following steps: and subtracting the endogenous respiration value of the microbial membrane from the exogenous respiration value to obtain the biochemical oxygen demand of the water sample to be detected.
The seawater measuring method based on seawater in-situ culture microbial membrane according to some embodiments of the invention further comprises a reactor for storing the aerated seawater sample and the water sample to be measured. And (3) carrying out microbial culture on the seawater sample containing the microorganisms in the reactor, and forming a microbial film on the inner wall of the reactor to obtain the microbial film reactor.
According to the seawater measurement method based on seawater in-situ culture microbial membrane of some embodiments of the present invention, the reactor is made of polylactic acid material. The shape, material and size of the reactor have no special requirements, the material of the reactor can be polylactic acid, glass, silica gel, nylon, plastic, quartz or ethylene-vinyl acetate copolymer, and the preferred material is polylactic acid.
According to the seawater measurement method based on seawater in-situ culture microbial membrane of some embodiments of the invention, the reactor is a hollow prism-like tubular structure. The reactor may be hollow prismatic, tubular or helical, preferably hollow prismatic.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram of a seawater measurement method based on seawater in-situ culture of microbial membranes according to the present embodiment;
FIG. 2 is a diagram illustrating the measurement of the respiration values of the microbial membrane according to the present embodiment;
fig. 3 is a schematic diagram of measuring the exogenous respiration value of the microbial membrane of the water sample to be measured according to the embodiment.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1, the invention provides a seawater measurement method based on seawater in-situ culture microbial film with the above functions. The method comprises the following steps: s100: carrying out microbial culture on the aerated seawater sample to obtain a microbial film; s200: measuring the endogenous respiration value of the microbial membrane; s300: measuring the microbial film exogenous respiration value of a water sample to be measured; s400: and measuring the biochemical oxygen demand of the water sample to be measured. The seawater measurement method based on seawater in-situ culture microbial membrane achieves the technical effect of simple and reliable measurement. The biochemical oxygen demand of the water sample to be detected can be detected only by adopting a single oxygen electrode, the detection process is simple and effective, and the preparation and use cost of the equipment is reduced. The water sample containing the microorganisms adopts seawater in different regions, the microorganisms cultured in situ in the seawater have strong environmental adaptability and are not influenced by seawater with high salt content and artificial seawater, and meanwhile, the seawater sample to be detected is used as a background to prepare a standard solution, so that compared with the method of preparing a phosphate buffer solution, the detection effect is more accurate, the error in detection is reduced, and the preparation of the buffer solution and secondary pollution to the environment are avoided.
Referring to fig. 2, the seawater measurement method based on seawater in situ culture of microbial membranes in some embodiments of the present invention measures microbial membrane endogenous respiration values, including: s201: enabling the seawater with the preset BOD value to flow through an oxygen electrode to obtain the initial oxygen content of the seawater; s202: the seawater with a preset BOD value flows through the microbial membrane and the oxygen electrode to obtain the oxygen content of the microbial membrane after endogenous respiration; s203: and subtracting the oxygen content after the microbial membrane endogenous respiration from the initial oxygen content to obtain the microbial membrane endogenous respiration value.
Referring to fig. 3, the seawater measurement method based on seawater in-situ culture of microbial membranes in some embodiments of the present invention for measuring the exogenous respiration value of microbial membranes of a water sample to be measured includes: s301: the water sample to be detected passes through the microbial membrane and the surface of the oxygen electrode in sequence to obtain the oxygen content after oxygen consumption; s302: directly passing a water sample to be detected through an oxygen electrode to obtain the initial oxygen content of the water sample; s303: and subtracting the initial oxygen content from the oxygen content after oxygen consumption to obtain the microbial film exogenous respiration value of the water sample to be detected.
Referring to fig. 1, the seawater measurement method based on seawater in-situ culture microbial film in some embodiments of the present invention, for measuring the biochemical oxygen demand of a water sample to be measured, includes: and subtracting the endogenous respiration value of the microbial membrane from the exogenous respiration value to obtain the biochemical oxygen demand of the water sample to be detected.
Referring to fig. 1, the seawater measurement method based on seawater in-situ culture of microbial membranes in some embodiments of the present invention further includes a reactor for storing the aerated seawater sample and the sample to be measured. And (3) carrying out microbial culture on the seawater sample containing the microorganisms in the reactor, and forming a microbial film on the inner wall of the reactor to obtain the microbial film reactor.
Referring to fig. 1, in the seawater measurement method based on seawater in-situ culture of microbial membranes according to some embodiments of the present invention, a reactor is made of a polylactic acid material. The shape, material and size of the reactor have no special requirements, and the material of the reactor can be polylactic acid, glass, silica gel, nylon, plastic, quartz or ethylene-vinyl acetate copolymer, preferably polylactic acid.
Referring to fig. 1, the seawater measurement method reactor based on seawater in-situ culture of microbial films according to some embodiments of the present invention has a hollow prism-like tubular structure. The reactor may be hollow prismatic, tubular or helical, preferably hollow prismatic.
Preferably, a microbial membrane reactor is prepared, one end of the microbial membrane reactor is connected with an oxygen electrode, the oxygen electrode is connected into an electrochemical workstation with the model of CHI852b, and the electrochemical workstation monitors the current change of the oxygen electrode and displays the current change on a computer. And opening the constant-temperature water bath kettle, adjusting the temperature to 37 ℃, injecting a 500mL water sample of the Huangmao sea water into the water sample container, and aerating the water sample at the rate of 3.5L/min. A polylactic acid reactor with the length of 100cm and the inner diameter of 3mm is placed in a constant-temperature water bath, a seawater sample passes through a sample inlet pipe and a three-way valve at the speed of 3.3mL/min and reaches the reactor, and then reaches the surface of an oxygen electrode through an oxygen electrode water inlet pipe. Continuously replenishing a seawater sample into a water sample container, culturing for 160h, injecting 250mL of artificial seawater into the artificial seawater sample container, aerating at the rate of 3.5L/min, passing the seawater through a first sample inlet pipe and a three-way valve by using a peristaltic pump at the rate of 3.3mL/min to reach the microbial membrane reactor, passing the seawater through an oxygen electrode water inlet pipe to reach the surface of an oxygen electrode, recording the dissolved oxygen reduction current to 275nA, and enabling the BOD concentration to be 5mgO2/LThe glucose glutamic acid solution is injected into a standard solution container and aerated at the speed of 3.5L/min, the glucose glutamic acid solution is pumped by a peristaltic pump to pass through a sample inlet pipe and a three-way valve at the speed of 3.3mL/min to reach a microbial membrane reactor and then to reach the surface of an oxygen electrode through an oxygen electrode water inlet pipe, an electrochemical workstation monitors the change of the current of the oxygen electrode, when the current of the oxygen electrode is stable, the redox current of the solution is recorded to be 230nA, the difference value of the dissolved oxygen reduction current of the artificial seawater passing through the surface of the oxygen electrode and the glucose glutamic acid solution passing through the surface of the oxygen electrode is calculated, namely 275 minus 230 plus 45nA, and the difference value represents that the BOD concentration is 5.0mgO2The signal response of the/L glucose glutamic acid solution on the obtained primary microbial membrane reactor. And repeating the culture step of the microbial membrane reactor until the difference value of multiple measurements is stable, indicating that saturated microorganisms are attached to the inner wall of the reactor, and judging that the culture of the microbial membrane reactor is finished to obtain the stable microbial membrane reactor.
Preferably, the thermostatic water bath is opened and the temperature is adjusted to 37 ℃. 250mL of the solution was poured into an artificial seawater vessel and aerated at a rate of 3.5L/min. Fully aerated artificial seawater is delivered to the surface of an oxygen electrode through a sample inlet pipe, a three-way valve and an oxygen electrode water inlet pipe at the speed of 3.3mL/min by using a peristaltic pump, an electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the dissolved oxygen reduction current is recorded as 388 nA. And then, aerating artificial seawater passes through the sampling pipe and the three-way valve at the speed of 3.3mL/min by using a peristaltic pump to reach the microbial membrane reactor, the artificial seawater reaches the surface of the oxygen electrode through the oxygen electrode water inlet pipe, the electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the dissolved oxygen reduction current is recorded as 347 nA. And calculating the difference value of the dissolved oxygen reduction current of the artificial seawater passing through the surface of the oxygen electrode and the artificial seawater passing through the microbial membrane reactor to reach the surface of the oxygen electrode, namely 388-347-41 nA, wherein the difference value represents the endogenous respiration current value of the microbial membrane.
Preferably, the thermostatic water bath is opened and the temperature is adjusted to 37 ℃. 250mL of the citronella sea water is injected into the sea water container to be tested, and aeration is carried out at the speed of 3.5L/min. Fully aerated seawater of the citronella sea is delivered to the microbial membrane reactor through the sampling pipe and the three-way valve at the speed of 3.3mL/min by using a peristaltic pump, and is delivered to the surface of an oxygen electrode through an oxygen electrode water inlet pipe, an electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the reduction current of dissolved oxygen is recorded as 281 nA. 150mg of glucose and 150mg of glutamic acid are dissolved in pure water, and the volume is determined to be 100mL, thus obtaining the mother liquor of the glucose glutamic acid solution with BOD concentration of 2000.0mgO 2/L. Diluting the mother liquor with seawater of the citronella sea to obtain glucose glutamic acid solutions with BOD of 1.0mgO2/L, 2.0mgO2/L, 4.0mgO2/L, 6.0mgO2/L and 8.0mgO2/L respectively. First, a glucose glutamic acid solution having a BOD concentration of 1.0mgO2/L was poured into 250mL of a standard solution vessel and aerated at a rate of 3.5L/min. And (3) passing the aerated glucose glutamic acid solution through a sampling pipe and a three-way valve at the speed of 3.3mL/min by using a peristaltic pump to reach the microbial membrane reactor, passing the aerated glucose glutamic acid solution through an oxygen electrode water inlet pipe to reach the surface of an oxygen electrode, monitoring the current change of the oxygen electrode by using an electrochemical workstation, and recording the reduction current of dissolved oxygen to be 270nA when the current of the oxygen electrode is stable. The difference of the reduction current of the dissolved oxygen of the seawater of the citronella sea and the glucose glutamic acid solution passing through the oxygen electrode surface is calculated, namely 281-270 to 11nA, and the difference represents the signal response of the glucose glutamic acid solution with the BOD concentration of 1.0mgO2/L on the obtained microbial membrane reactor. And (4) repeating the washing step of the seawater of the citronella, recording the reduction current value of the dissolved oxygen when the oxygen electrode is stable, and then sequentially detecting glucose glutamic acid solutions with other BOD concentrations. According to the difference, the BOD concentration value of the glucose glutamic acid solution with the BOD concentration of 1.0mgO2/L-8.0mgO2/L is plotted as an abscissa, and the corresponding oxygen electrode signal response is plotted as an ordinate, and a standard curve of the glucose glutamic acid solution is plotted.
Preferably, the thermostatic water bath is opened and the temperature is adjusted to 37 ℃. 250mL of the citronella seawater is injected into the seawater container to be tested, and aeration is carried out at the speed of 3.5L/min. Fully aerated seawater to be detected reaches the surface of the oxygen electrode through the sampling pipe, the three-way valve and the oxygen electrode water inlet pipe at the speed of 3.3mL/min by using a peristaltic pump, an electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the dissolved oxygen reduction current is recorded as 316 nA. And then the peristaltic pump is used for leading the aerated artificial seawater to pass through the sampling pipe and the three-way valve at the speed of 3.3mL/min to reach the microbial membrane reactor, and then the aerated artificial seawater reaches the surface of the oxygen electrode through the oxygen electrode water inlet pipe, the electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the dissolved oxygen reduction current is recorded to be 248 nA. And calculating the difference value of the dissolved oxygen reduction current of the citicoline seawater passing through the oxygen electrode surface and the oxygen electrode surface through the microbial membrane, namely 316-. The BOD value of the water sample of the seawater of the citronella is calculated to be 2.8mgO 2/L.
Preferably, the thermostatic water bath is opened and the temperature is adjusted to 37 ℃. 250mL of the solution was poured into an artificial seawater vessel and aerated at a rate of 3.5L/min. Fully aerated artificial seawater is delivered to the surface of an oxygen electrode through a sample inlet pipe, a three-way valve and an oxygen electrode water inlet pipe at the speed of 3.3mL/min by using a peristaltic pump, an electrochemical workstation monitors the current change of the oxygen electrode, and when the current of the oxygen electrode is stable, the dissolved oxygen reduction current is recorded as 288 nA. Diluting the glucose glutamic acid solution with artificial seawater to obtain glucose glutamic acid standard solutions with BOD concentration of 5.0mgO2/L, respectively, injecting 250mL into the standard solution container, and aerating at rate of 3.5L/min. And (3) passing the aerated glucose glutamic acid solution through a sampling pipe and a three-way valve at the speed of 3.3mL/min by using a peristaltic pump to reach the microbial membrane reactor, passing the aerated glucose glutamic acid solution through an oxygen electrode water inlet pipe to reach the surface of an oxygen electrode, monitoring the current change of the oxygen electrode by using an electrochemical workstation, and recording the reduction current of dissolved oxygen as 241nA when the current of the oxygen electrode is stable. The difference of the dissolved oxygen reduction current of the artificial seawater passing through the oxygen electrode surface and the glucose glutamic acid solution passing through the oxygen electrode surface is calculated, namely 288-. The above experiment was repeated 3 times every other day for sixty days to test the stability of the microbial membrane reactor. The measurement results are all about 5.0mgO2/L glucose glutamic acid standard solution, which shows that the microbial membrane reactor has higher stability and accurate measurement results.
Preferably, the temperature of the constant temperature water bath is adjusted to 25 ℃, 30 ℃, 35 ℃, 37 ℃ and 40 ℃ respectively for testing the current response of the microbial membrane reactor at different temperatures. The temperature of the microbial membrane reactor is preferably 37 ℃ because the response of the microbial membrane reactor is gradually increased along with the increase of the temperature, the response is reduced when the temperature reaches 40 ℃, and the activity of microorganisms is influenced due to overhigh temperature.
Preferably, the salinity of the artificial seawater is adjusted to 0mol/L, 0.4mol/L, 0.7mol/L, 1.0mol/L and 2.0mol/L respectively for testing the current response of the microbial membrane reactor at different salinity. The current response of the microbial membrane reactor is maximum at the salinity of 0.4mol/L, and then gradually decreases. At 2.0mol/L, the current response increases, and in order to save material cost, the salinity of the microbial membrane reactor is preferably 0.4 mol/L.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (7)
1. A seawater measurement method based on seawater in-situ culture microbial film is characterized by comprising the following steps:
carrying out microbial culture on the aerated seawater sample to obtain a microbial film;
measuring the microbial membrane endogenous respiration values;
measuring the microbial film exogenous respiration value of a water sample to be measured;
and measuring the biochemical oxygen demand of the water sample to be measured.
2. The seawater measurement method based on seawater in-situ culture microbial membranes as claimed in claim 1, wherein the measuring of the microbial membrane endogenous respiration value comprises:
enabling the seawater with the preset BOD value to flow through an oxygen electrode to obtain the initial oxygen content of the seawater;
the seawater with a preset BOD value flows through the microbial membrane and the oxygen electrode to obtain the oxygen content of the microbial membrane after endogenous respiration;
and subtracting the oxygen content after the microbial membrane endogenous respiration from the initial oxygen content to obtain the microbial membrane endogenous respiration value.
3. The seawater measurement method based on seawater in-situ culture microbial membrane of claim 1, wherein the measuring of the microbial membrane exogenous respiration value of the water sample to be measured comprises:
the water sample to be detected passes through the microbial film and the surface of the oxygen electrode in sequence to obtain the oxygen content after oxygen consumption;
directly passing a water sample to be detected through the oxygen electrode to obtain the initial oxygen content of the water sample;
and subtracting the initial oxygen content from the oxygen content after oxygen consumption to obtain the microbial film exogenous respiration value of the water sample to be detected.
4. The seawater measurement method based on seawater in-situ culture microbial membrane of claim 1, wherein the measuring of the biochemical oxygen demand of the water sample to be measured comprises:
and subtracting the endogenous respiration value of the microbial membrane from the exogenous respiration value to obtain the biochemical oxygen demand of the water sample to be detected.
5. The seawater measurement method based on seawater in-situ culture microbial membranes as claimed in claim 1, further comprising a reactor for storing the aerated seawater sample and the water sample to be tested.
6. The seawater measurement method based on seawater in-situ culture microbial membranes as claimed in claim 5, wherein the reactor is made of polylactic acid material.
7. The seawater measurement method based on seawater in situ culture microbial membranes of claim 5, wherein the reactor is a hollow prism-like tubular structure.
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