CN113457756A

CN113457756A - Biological sample transports storage cup

Info

Publication number: CN113457756A
Application number: CN202110748606.3A
Authority: CN
Inventors: 周标; 陈小勇; 蒋征刚; 张治�; 李婵; 胡崇高; 郑银霜; 吴晓丽
Original assignee: Hangzhou Yikai Biotechnology Co ltd; Zhejiang Center for Disease Control and Prevention
Current assignee: Hangzhou Yikai Biotechnology Co ltd; Zhejiang Center for Disease Control and Prevention
Priority date: 2021-07-02
Filing date: 2021-07-02
Publication date: 2021-10-01

Abstract

The invention relates to the field of biological sample transportation, in particular to a biological sample transportation and storage cup which comprises a cup body and a cup cover, wherein an insulating layer is arranged on the upper part of the cup body, a biological sample test tube storage tank is arranged in the middle of the insulating layer, and a biological sample sterilizing and killing device is arranged on the side wall of the storage tank; an opening is formed in the bottom of the storage tank, a sensor for detecting whether a test tube exists in the biological sample test tube storage tank is arranged in the opening, the sensor is fixedly installed at the bottom of the heat insulation layer, and a lead-out wire of the sensor is electrically connected with the main PCB; an annular groove is arranged between the upper connecting ring and the lower connecting ring, a transparent or semitransparent annular body is arranged in the annular groove, the outer diameter of the annular body is the same as that of the upper cup body and the lower cup body, an LED display lamp is arranged on the main PCB, and the color of the LED display lamp is changed after a sample is placed in the annular body, so that the color of the annular body is correspondingly changed. This patent is convenient for follow the in appearance of cup through the cooperation of sensor, main PCB board, LED lamp and ring body, and whether the biological sample has been stored to the inside of observation cup.

Description

Biological sample transports storage cup

Technical Field

The invention relates to the field of biological sample transportation, in particular to a biological sample transportation storage cup.

Background

Biological samples include biological macromolecules, cells, organ tissues, whole blood, plasma, serum, biological fluids or treated DNA, RNA, proteins, etc. of disease organisms collected on a standardized basis. When transporting these biological samples, a biological sample transport box meeting the national standard must be used. The existing transfer box generally comprises a closed box body and a biological sample container arranged in the box body, and a refrigerating device is arranged in the box body to prolong the preservation time of the biological sample.

The existing biological sample containers only have basic storage functions, and whether a biological sample is stored in the container cannot be determined from the appearance.

Disclosure of Invention

In order to solve the above problems, an object of the present invention is to provide a biological sample transportation and storage cup, wherein an insulating layer is disposed on a cup body, a biological sample test tube storage tank is disposed in the middle of the insulating layer, an opening is disposed at the bottom of the storage tank, a sensor is disposed in the opening, the sensor detects whether a test tube is present in the storage tank and transmits a signal to a main PCB, the main PCB causes an LED lamp to emit light, a transparent or semitransparent annular body is disposed on the outer side of the LED lamp, and the annular body is disposed on the cup body to cause the cup body to emit light, thereby facilitating observation of whether a biological sample is stored in the cup.

For the purpose of the invention, the following technical scheme is adopted for implementation:

a biological sample transferring and storing cup comprises a cup body and a cup cover, wherein a heat insulation layer is arranged on the upper portion of the cup body, a biological sample test tube storage tank is arranged in the middle of the heat insulation layer, and a biological sample sterilizing and killing device is arranged on the side wall of the storage tank; an electronic control unit is arranged at the lower part of the cup body and comprises a controller, a short-distance wireless communication unit and a battery, the short-distance wireless communication unit is connected with the controller, and the controller is connected with and controls the biological sample sterilizing device;

the cup body consists of an upper cup body, an upper connecting ring, a lower connecting ring and a lower cup body; the cup cover is fixed on the upper cup body in a screw thread way; the lower connecting ring is provided with a plurality of connecting columns, the upper connecting ring is fixed on the plurality of convex connecting columns through a plurality of screws, so that the lower connecting ring and the upper connecting ring form a middle isolation space, and a main PCB is fixedly arranged in the middle isolation space;

an opening is formed in the bottom of the storage tank, a sensor for detecting whether a test tube exists in the biological sample test tube storage tank is arranged in the opening, the sensor is fixedly installed at the bottom of the heat insulation layer, and a lead-out wire of the sensor is electrically connected with the main PCB;

an annular groove is arranged between the upper connecting ring and the lower connecting ring, a transparent or semitransparent annular body is arranged in the annular groove, the outer diameter of the annular body is the same as that of the upper cup body and the lower cup body, an LED display lamp is arranged on the main PCB, and the color of the LED display lamp is changed after a sample is placed in the annular body, so that the color of the annular body is correspondingly changed.

Preferably, the lower connecting ring is fixedly connected with the lower cup body, a storage cavity is arranged in the lower cup body, the battery is arranged in the storage cavity, the battery is connected to the main PCB through an electric connection interface, and a charging interface and a switch device are arranged at the bottom of the lower cup body.

Preferably, the upper cup body is fixedly connected with the upper connecting ring, and the heat insulation layer is arranged in the upper cup body; the biological sample sterilizing device is arranged in the heat preservation layer; the biological sample sterilizing device comprises a heating pipe which is vertically arranged, the bottom of the heating pipe is fixedly connected with the upper connecting ring, and a lead wire led out from the heating pipe is electrically connected with the main PCB; the biological sample test tube storage tank is formed inside the heating tube.

Preferably, the upper part of the heat-insulating layer is provided with a test tube bayonet.

Preferably, the biological sample transfer and storage cup is also provided with an acoustic and optical prompting device when the biological sample is loaded.

Preferably, the biological sample sterilizing device is selected from one of heat sterilization, biochemical sterilization, ultraviolet sterilization and heat-combined biochemical sterilization.

Preferably, the short-distance wireless communication unit adopts Bluetooth, wireless broadband and near field communication.

In conclusion, the cup has the advantages that the sensor, the main PCB, the LED lamp and the annular body are matched, so that whether the biological sample is stored in the cup body or not can be observed conveniently from the appearance of the cup body. Set up biological sample on the heat preservation lateral wall and disappear and kill the device, be convenient for when emergency takes place, disappear and kill to biological sample.

Drawings

Fig. 1 is a flow chart of a biological sample intelligent security cloud protection system.

Fig. 2 and 3 are schematic structural views of the outer box.

Fig. 4 is a schematic structural diagram of the inner box for placing the biological sample transfer storage cup and the intelligent monitor.

FIG. 5 is a schematic structural view of the inner case after the biological sample transfer and storage cup and the intelligent monitor are removed.

Fig. 6 is a schematic structural view of the inner box cover.

FIG. 7 is a schematic structural diagram of a biological sample transfer and storage cup.

Fig. 8 is a sectional view taken along the direction a-a in fig. 7.

Fig. 9 is an exploded view of a biological sample transfer and storage cup.

Figure 10 is an exploded view of the upper and lower attachment rings, the main PCB board and the annular body.

FIG. 11 is a schematic structural view of the insulation layer, the heating tube and the sensor.

Detailed Description

As shown in fig. 1, a biological sample intelligent security cloud protection system includes: a class biological safety transfer box, a biological sample transfer storage cup 2, an intelligent monitor 3 and a remote monitoring system. The A-type biological safety transfer box meets the UN2814 standard.

As shown in fig. 2 to 6, the transfer box comprises an inner box 11 and an outer box 12, the inner box 11 is made of a high polymer heat-insulating buffer material, the outer box 12 is made of an ultralight aviation aluminum-magnesium alloy, and the outer box 12 is provided with a dynamic electronic coded lock which can be an intelligent lock such as a customs coded lock, a random coded lock, a fingerprint lock and a face recognition lock, so that the biosafety transfer box is ensured to have the intelligent and safe opening functions. The inner case 11 is provided with a cup groove 111 for fixedly placing the biological sample transfer/storage cup 2.

The inner box 11 is provided with a cup groove 111, a refrigerating device 112 and a smart monitor slot 113. A plurality of (four in fig. 3) cooling devices 112 are provided on the side wall of the inner box 11. A plurality of (eight in fig. 4) cup recesses 111 are provided at the bottom of the inner case 111 and form at least two cup recess areas. The smart monitor slot 113 is disposed at the bottom of the inner case 111 and between the cup slots. The upper end surface of the inner box 11 is provided with a groove 114 for positioning the inner box cover 115. An inner case cover 115 is provided on the upper portion of the inner case 11, and a flange 1151 corresponding to the groove 114 is provided on the bottom of the inner case cover 115 to facilitate mounting of the inner case cover 115 to the inner case 111. The bottom of interior case lid 115 still is equipped with the first positioning groove 1152 that corresponds with cup groove 111, transports storage cup 2 from the top and the below simultaneously to biological sample and fixes, prevents that biological sample from transporting storage cup 2 and taking place to remove when transporting, better save biological sample. The bottom of inner box cover 115 still is equipped with the second constant head tank 1153 that corresponds with intelligent monitor slot 113, is convenient for fix intelligent monitor 3. Grip grooves 1154 are formed on both sides of the inner cover 115 to facilitate gripping of the inner cover 115 when opening.

The outer case 12 includes an outer case 121 and an outer case cover 122, and the inner case 11 is accommodated in the outer case 121. One side end of the outer cover 122 is hinged to one side of the upper part of the outer box 121, so that the outer cover 122 can be opened conveniently. The other side of the outer case cover 122 is provided with a first locking member 1221, and the outer case 121 is provided with a second locking member 1211 corresponding to the first locking member 1221. The pull ring 1222 is rotatably disposed on the top of the outer case cover 122 for facilitating grasping of the outer case 12 for transportation.

As shown in fig. 7 and 8, the biological sample transferring and storing cup 2 comprises a cup body 21 and a cup cover 22, wherein an insulating layer 23 is arranged on the upper part of the cup body 21, a biological sample test tube storage tank 231 is arranged in the middle of the insulating layer 23, and a biological sample sterilizing and killing device is arranged on the side wall of the storage tank 231. The biological sample killing device is selected from one of heating killing, biochemical killing, ultraviolet killing and heating and biochemical killing. The heating mode adopts the combination of a high-heat-conduction material and a lithium battery with high energy density, so that the biological sample sterilizing device reaches a high-temperature interval of 100 ℃ within 7 minutes, and is kept for at least 5 minutes to be heated continuously, and the aim of effectively sterilizing the biological sample is fulfilled. The heat-insulating layer 23 is made of a novel material to prevent heat from overflowing. The biological sample sterilizing device is designed by IP67 with high protection grade and can endure 95Kpa pressure difference without leakage. The surface treatment of the cup body 21 has the bacteriostatic ability, and the material is easy to sterilize after each use.

The lower part of the cup body 21 is provided with an electronic control unit which comprises a controller, a short-distance wireless communication unit and a battery 24, wherein the short-distance wireless communication unit is connected with the controller, and the controller is connected with and controls the biological sample sterilizing device. The wireless communication connection is stable and reliable, and the security authentication capability is provided. The single-time electricity endurance of the biological sample transferring and storing cup 2 is not less than 365 days.

As shown in fig. 7 to 11, the cup 21 is composed of an upper cup 211, an upper connection ring 212, a lower connection ring 213, and a lower cup 214. The cap 22 is screwed to the upper cup 211 by means of a screw thread. The lower connection ring 213 is provided with a plurality of connection columns 2131, the upper connection ring 212 is fixed on the plurality of protruding connection columns 2131 by a plurality of screws, so that the lower connection ring 213 and the upper connection ring 212 form a middle isolation space, and one main PCB 25 is fixedly arranged in the middle isolation space. The lower connecting ring 213 is fixedly connected with the lower cup 214, a storage cavity is arranged in the lower cup 214, the battery 24 is arranged in the storage cavity, the battery 24 is connected to the main PCB 25 through the electric connecting interface 241, and the charging interface 26 and the switch device 27 are arranged at the bottom of the lower cup 214.

The upper cup body 211 is fixedly connected with the upper connecting ring 212, the heat-insulating layer 23 is arranged in the upper cup body 211, and the biological sample sterilizing device is arranged in the heat-insulating layer 23. The biological sample sterilizing device comprises a vertically arranged heating pipe 28, and the heating pipe 28 is made of novel materials with high heat-conducting property. The bottom of the heating tube 28 is fixedly connected with the upper connecting ring 212, and the lead wire led out from the heating tube 28 is electrically connected with the main PCB 25. A biological sample tube reservoir 231 is formed inside the heating tube 28.

The bottom of heating pipe 28 is equipped with the opening, is provided with in the opening and is used for detecting whether there is the sensor 281 of test tube in biological sample test tube storage tank 231, and sensor 281 fixed mounting is in the bottom of heat preservation 23, and sensor 281 draws the wire and is connected with main PCB board 25 mutually the electricity. The upper part of the heat preservation layer 23 is provided with a test tube bayonet 232.

The biological sample transferring and storing cup 2 is also provided with an acoustic and optical prompting device when loading the biological sample. An annular groove is arranged between the upper connecting ring 212 and the lower connecting ring 213, a transparent or semitransparent annular body 29 is arranged in the annular groove, the outer diameter of the annular body 29 is the same as that of the upper cup body 211 and the lower cup body 214, an LED display lamp 251 is arranged on the main PCB 25, and the color of the LED display lamp 251 is changed after a sample is placed, so that the color of the annular body 29 is changed correspondingly.

Intelligent monitoring ware 3 sets up in the transfer box, and intelligent monitoring ware 3 includes temperature and humidity detector, unpacking alarm unit, multimode positioning unit, striking alarm unit and remote wireless communication unit. The electronic control unit is connected with the intelligent monitor 3 through the short-distance wireless communication unit, the intelligent monitor 3 collects the temperature and humidity, the illumination intensity, the vibration, the air pressure and the positioning data in the transfer box, the collected data are uploaded to a remote monitoring system through the long-distance wireless communication unit in a block chain technology in real time, and collected environment parameters are guaranteed to be capable of being tampered.

Remote monitering system carries out stable data communication with intelligent monitoring ware 3, ensures that biological sample safety is controllable in biological sample transports memory cup 2, in the proruption emergency situation of biological sample, gives the instruction and transmits for

intelligent monitoring ware

3, and 3 control electronic control units of intelligent monitoring ware start biological sample device of killing destroys biological sample.

The intelligent monitor 3 has high-sensitivity temperature detection (+/-0.5 ℃ temperature error) and high-sensitivity humidity detection (+/-5% RH humidity error), and the single-charge cruising ability is not less than 12 days. The communication needs to be able to effectively penetrate through signal shielding, stably transmit signals, and avoid data loss, and the communication frequency and the sampling frequency can be set according to requirements.

The multi-module positioning unit comprises one or more combinations of a Beidou positioning system, a GPS, a GLONASS, a Galileo, a wifi and a base station, and the global logistics track tracing is realized.

The near-field wireless communication unit selects one of Zig-Bee, Bluetooth (Bluetooth), wireless broadband (Wi-Fi), ultra-wideband (UWB) and Near Field Communication (NFC). The long-distance wireless communication unit adopts 5G communication.

The long-distance wireless communication unit can also adopt one of GPRS/CDMA, data transmission radio station communication, spread spectrum microwave communication, wireless network bridge, satellite communication and short-wave communication.

An intelligent and safe cloud protection system for biological samples adopts a SaaS framework, relies on mature middleware, and supports a server through a plurality of data centers to ensure that collected data cannot be tampered. The service availability is 99.9%, the data reliability is 99.9%, the long connection of tens of millions of devices is supported, millions of devices are concurrent, and the SaaS cloud platform is highly stably expressed with extremely low time delay from the end to the cloud.

The system can effectively supervise, protect and eliminate biological safety risks by aiming at the risks that highly pathogenic microbe samples of BSL-3 to BSL-4 grades are abnormal in storage and transportation environment, lost or leaked in the transportation process and the like through a three-layer protection framework (biological safety is visual, can be prevented and controlled), namely a visual management system platform, a special biological safety transfer box with an intelligent opening function and remote virus controllable sterilization under the abnormal condition of the biological samples.

Claims

1. A biological sample transfer storage cup is characterized by comprising a cup body (21) and a cup cover (22), wherein an insulating layer (23) is arranged at the upper part of the cup body (21), a biological sample test tube storage tank (231) is arranged in the middle of the insulating layer (23), and a biological sample killing device is arranged on the side wall of the storage tank (231); an electronic control unit is arranged at the lower part of the cup body (21), the electronic control unit comprises a controller, a short-distance wireless communication unit and a battery (24), the short-distance wireless communication unit is connected with the controller, and the controller is connected with and controls the biological sample sterilizing device;

the cup body (21) is composed of an upper cup body (211), an upper connecting ring (212), a lower connecting ring (213) and a lower cup body (214); the cup cover (22) is fixed on the upper cup body (211) in a threaded manner; the lower connecting ring (213) is provided with a plurality of connecting columns (2131), the upper connecting ring (212) is fixed on the plurality of convex connecting columns (2131) by a plurality of screws, so that a middle isolation space is formed by the lower connecting ring (213) and the upper connecting ring (212), and a main PCB (25) is fixedly arranged in the middle isolation space;

an opening is formed in the bottom of the storage tank (231), a sensor (281) for detecting whether a test tube exists in the biological sample test tube storage tank (231) is arranged in the opening, the sensor (281) is fixedly installed at the bottom of the heat preservation layer (23), and a lead wire led out from the sensor (281) is electrically connected with the main PCB (25);

an annular groove is formed between the upper connecting ring (212) and the lower connecting ring (213), a transparent or semitransparent annular body (29) is arranged in the annular groove, the outer diameter of the annular body (29) is the same as that of the upper cup body (211) and the lower cup body (214), an LED display lamp (251) is arranged on the main PCB (25), and the LED display lamp (251) changes color to enable the annular body (29) to change color correspondingly after a sample is placed in the LED display lamp (251).

2. The biological sample transfer and storage cup according to claim 1, wherein the lower connection ring (213) is fixedly connected to the lower cup body (214), a storage cavity is formed in the lower cup body (214), the battery (24) is disposed in the storage cavity, the battery (24) is connected to the main PCB (25) through the electrical connection interface (241), and the charging interface (26) and the switch device (27) are disposed at the bottom of the lower cup body (214).

3. The biological sample transfer and storage cup as claimed in claim 1, wherein the upper cup body (211) is fixedly connected with the upper connecting ring (212), and the insulating layer (23) is arranged in the upper cup body (211); the biological sample sterilizing device is arranged inside the heat preservation layer (23); the biological sample sterilizing device comprises a heating pipe (28) which is vertically arranged, the bottom of the heating pipe (28) is fixedly connected with an upper connecting ring (212), and a lead wire led out of the heating pipe (28) is electrically connected with the main PCB (25); the biological sample test tube storage tank (231) is formed inside the heating tube (28).

4. The biological specimen transfer and storage cup according to claim 1, wherein the upper portion of the heat insulation layer (23) is provided with a test tube bayonet (232).

5. The biological specimen transport and storage cup according to claim 1, wherein the biological specimen transport and storage cup further comprises an audible and visual indicator for loading the biological specimen.

6. The biological specimen transport and storage cup of claim 1, wherein the biological specimen killing means is selected from the group consisting of heat killing, biochemical killing, uv killing, and heat-coupled biochemical killing.

7. The biological sample transport and storage cup of claim 1, wherein the short-range wireless communication unit is selected from bluetooth, wireless broadband and near field communication.