CN113447561A - MALDI-TOF detection method for sheep-derived components in milk powder - Google Patents
MALDI-TOF detection method for sheep-derived components in milk powder Download PDFInfo
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- CN113447561A CN113447561A CN202110903414.5A CN202110903414A CN113447561A CN 113447561 A CN113447561 A CN 113447561A CN 202110903414 A CN202110903414 A CN 202110903414A CN 113447561 A CN113447561 A CN 113447561A
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- milk powder
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/64—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using wave or particle radiation to ionise a gas, e.g. in an ionisation chamber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
The invention discloses a MALDI-TOF detection method for sheep-derived components in milk powder, which comprises the following steps: A. mixing a milk powder sample to be detected with indole-3-acetic acid and ethanol to prepare a matrix mixed solution; B. mixing a milk powder sample to be detected with tetrahydrofuran to prepare a sample mixed solution; C. dropwise adding the matrix mixed solution on a target spot of the bearing target plate to prepare a matrix layer, and then dropwise adding the sample mixed solution on the matrix layer to prepare a sample layer; D. the support target plate is sent to MALDI-TOF equipment for MALDI-TOF detection. The invention can improve the defects of the prior art and improve the accuracy of MALDI-TOF detection on the milk powder.
Description
Technical Field
The invention relates to the technical field of chemical component detection, in particular to a MALDI-TOF detection method for sheep-derived components in milk powder.
Background
Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) is a cocrystallized film formed by irradiating a sample and a matrix with laser, the matrix absorbs energy from the laser and transfers the energy to biomolecules, the protons are transferred to the biomolecules or obtained from the biomolecules in the ionization process to ionize the biomolecules, the ions accelerate to fly through a flight tube under the action of an electric field, and the ions are detected according to different flight times of a detector, namely the mass-to-charge ratio of the ions is determined to be in direct proportion to the flight time of the ions, so that the ion detection is realized. The monitoring mode has the advantages of high sensitivity, high resolution, short detection time and the like, and is widely applied to analysis of various sample components. When the milk powder is subjected to MALDI-TOF detection, because the milk powder sample has higher requirements on the matrix, obvious matrix interference is easy to occur, and the accuracy of the MALDI-TOF detection of the milk powder is influenced.
Disclosure of Invention
The invention aims to solve the technical problem of providing a MALDI-TOF detection method for sheep-derived components in milk powder, and improving the accuracy of MALDI-TOF detection of the milk powder.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows.
A MALDI-TOF detection method for sheep-derived components in milk powder comprises the following steps:
A. mixing a milk powder sample to be detected with indole-3-acetic acid and ethanol to prepare a matrix mixed solution;
B. mixing a milk powder sample to be detected with tetrahydrofuran to prepare a sample mixed solution;
C. dropwise adding the matrix mixed solution on a target spot of the bearing target plate to prepare a matrix layer, and then dropwise adding the sample mixed solution on the matrix layer to prepare a sample layer;
D. the support target plate is sent to MALDI-TOF equipment for MALDI-TOF detection.
Preferably, in the step A, the mass ratio of the milk powder sample, the indole-3-acetic acid and the ethanol is 1:150:300, and the milk powder sample, the indole-3-acetic acid and the ethanol are mixed and then treated by ultrasonic waves for 1min for later use.
Preferably, in step B, the sample mixed solution is subjected to desalting treatment and impurity removal treatment.
Preferably, in step C, the target plate is heated to 60 ℃, then the substrate mixed solution is dripped on the target, the target plate is cooled to room temperature after the substrate mixed solution is dried, then the sample mixed solution is dripped on the surface of the substrate, and the substrate is naturally dried.
Preferably, a plurality of target points are arranged on the surface of the bearing target plate in an array form, each target point comprises an upper layer and a lower layer, the diameter of the upper layer is larger than that of the lower layer, the dropping liquid level of the matrix mixed solution is positioned below the upper edge of the lower layer, and the dropping liquid level of the sample mixed solution is positioned below the upper edge of the upper layer.
Preferably, the side wall of the lower layer is provided with a heat insulation layer.
Adopt the beneficial effect that above-mentioned technical scheme brought to lie in: the invention effectively reduces the influence of matrix interference on the detection result by optimizing the preparation components and the preparation process of the base layer. Meanwhile, the quality of manufacturing the substrate layer and the sample layer is improved by improving the structure of the bearing target plate, so that the detection accuracy is further improved.
Drawings
FIG. 1 is an enlarged view of a portion of a load bearing target plate.
In the figure: 1. carrying a target plate; 2. a target point; 3. an upper layer; 4. a lower layer; 5. an insulating layer.
Detailed Description
A MALDI-TOF detection method for sheep-derived components in milk powder comprises the following steps:
A. mixing a milk powder sample to be detected with indole-3-acetic acid and ethanol to prepare a matrix mixed solution;
B. mixing a milk powder sample to be detected with tetrahydrofuran to prepare a sample mixed solution;
C. dropwise adding the matrix mixed solution on the target spot 2 of the bearing target plate 1 to prepare a matrix layer, and then dropwise adding the sample mixed solution on the matrix layer to prepare a sample layer;
D. the support target plate 1 is sent to MALDI-TOF equipment for MALDI-TOF detection.
In the step A, the mass ratio of the milk powder sample to the indole-3-acetic acid to the ethanol is 1:150:300, and the milk powder sample, the indole-3-acetic acid and the ethanol are mixed and then treated by ultrasonic waves for 1min for later use.
And step B, carrying out desalination treatment and impurity removal treatment on the sample mixed solution.
In the step C, the bearing target plate 1 is heated to 60 ℃, then the matrix mixed solution is dripped on the target point 2, the bearing target plate 1 is cooled to room temperature after the matrix mixed solution is dried, then the sample mixed solution is dripped on the surface of the matrix, and the mixture is naturally dried.
A plurality of target points 2 are arranged on the surface of the bearing target plate 1 in an array mode, each target point 2 comprises an upper layer 3 and a lower layer 4, the diameter of the upper layer 3 is larger than that of the lower layer 4, the dropping liquid level of the matrix mixed solution is positioned below the upper edge of the lower layer 5, and the dropping liquid level of the sample mixed solution is positioned below the upper edge of the upper layer 3.
The side wall of the lower layer 4 is provided with a heat insulation layer 5.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A MALDI-TOF detection method for sheep-derived components in milk powder is characterized by comprising the following steps:
A. mixing a milk powder sample to be detected with indole-3-acetic acid and ethanol to prepare a matrix mixed solution;
B. mixing a milk powder sample to be detected with tetrahydrofuran to prepare a sample mixed solution;
C. dropwise adding the matrix mixed solution on the target spot (2) of the bearing target plate (1) to prepare a matrix layer, and then dropwise adding the sample mixed solution on the matrix layer to prepare a sample layer;
D. the bearing target plate (1) is sent to a MALDI-TOF device for MALDI-TOF detection.
2. The MALDI-TOF detection method for sheep-derived components in milk powder according to claim 1, characterized in that: in the step A, the mass ratio of the milk powder sample to the indole-3-acetic acid to the ethanol is 1:150:300, and the milk powder sample, the indole-3-acetic acid and the ethanol are mixed and then treated by ultrasonic waves for 1min for later use.
3. The MALDI-TOF detection method for sheep-derived components in milk powder according to claim 2, characterized in that: and step B, carrying out desalination treatment and impurity removal treatment on the sample mixed solution.
4. The MALDI-TOF detection method for sheep-derived components in milk powder according to claim 3, characterized in that: in the step C, the bearing target plate (1) is heated to 60 ℃, then the matrix mixed solution is dripped on the target point (2), the bearing target plate (1) is cooled to room temperature after the matrix mixed solution is dried, then the sample mixed solution is dripped on the surface of the matrix, and the mixture is naturally dried.
5. The MALDI-TOF detection method for sheep-derived components in milk powder according to claim 4, characterized in that: bear target plate (1) surface and arrange a plurality of target (2) with the array form, target (2) include upper strata (3) and lower floor (4), and the diameter of upper strata (3) is greater than the diameter of lower floor (4), and matrix mixed solution dropwise add liquid level is located lower floor (5) top edge below, and sample mixed solution dropwise add liquid level is located upper strata (3) top edge under.
6. The MALDI-TOF detection method for sheep-derived components in milk powder according to claim 5, characterized in that: and a heat insulation layer (5) is arranged on the side wall of the lower layer (4).
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| CN202110903414.5A CN113447561B (en) | 2021-08-06 | 2021-08-06 | MALDI-TOF detection method for sheep-derived components in milk powder |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770272A (en) * | 1995-04-28 | 1998-06-23 | Massachusetts Institute Of Technology | Matrix-bearing targets for maldi mass spectrometry and methods of production thereof |
| JP2001153842A (en) * | 1999-11-25 | 2001-06-08 | Mitsubishi Chemicals Corp | Surface analytic method |
| JP2002148157A (en) * | 2000-11-07 | 2002-05-22 | Mitsubishi Chemicals Corp | Surface analyzing method |
| US20160035553A1 (en) * | 2013-04-04 | 2016-02-04 | Keio University | Sample preparation method and sample preparation device for maldi |
| CN106404885A (en) * | 2015-07-28 | 2017-02-15 | 中国科学院化学研究所 | Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method |
| CN109776391A (en) * | 2018-12-21 | 2019-05-21 | 中山大学 | N- acrylic acid carbazole and its application as matrix in Matrix-assisted laser desorption ionization analyzing polymers |
-
2021
- 2021-08-06 CN CN202110903414.5A patent/CN113447561B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770272A (en) * | 1995-04-28 | 1998-06-23 | Massachusetts Institute Of Technology | Matrix-bearing targets for maldi mass spectrometry and methods of production thereof |
| JP2001153842A (en) * | 1999-11-25 | 2001-06-08 | Mitsubishi Chemicals Corp | Surface analytic method |
| JP2002148157A (en) * | 2000-11-07 | 2002-05-22 | Mitsubishi Chemicals Corp | Surface analyzing method |
| US20160035553A1 (en) * | 2013-04-04 | 2016-02-04 | Keio University | Sample preparation method and sample preparation device for maldi |
| CN106404885A (en) * | 2015-07-28 | 2017-02-15 | 中国科学院化学研究所 | Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method |
| CN109776391A (en) * | 2018-12-21 | 2019-05-21 | 中山大学 | N- acrylic acid carbazole and its application as matrix in Matrix-assisted laser desorption ionization analyzing polymers |
Non-Patent Citations (2)
| Title |
|---|
| 张涛等: "含乳特殊医学用途配方食品中沙门氏菌检测即用型质控样品研制", 《乳业科学与技术》, vol. 44, no. 4, 1 July 2021 (2021-07-01) * |
| 张涛等: "飞行时间质谱即用型金黄色葡萄球菌质控样品研究", 《食品安全质量检测学报》, no. 09, 15 May 2020 (2020-05-15) * |
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