CN113440479A - Nano emulsion of plant essential oil and preparation method and application thereof - Google Patents
Nano emulsion of plant essential oil and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a nano-emulsion preparation technology, and discloses a nano-emulsion of plant essential oil, a preparation method and an application thereof. The nanoemulsion of the plant essential oil provided by the invention contains patchouli essential oil, an emulsifier, an encapsulant, a cross-linking agent and water, wherein the content of the emulsifier is 1-60 parts by weight, the content of the encapsulant is 1-20 parts by weight, the content of the cross-linking agent is 0.1-10 parts by weight and the content of the water is 80-1000 parts by weight based on 10 parts by weight of the patchouli essential oil. The preparation method of the nano emulsion comprises the following steps: (1) mixing patchouli essential oil, an emulsifier, an encapsulant and a cross-linking agent, and homogenizing to obtain a mixture; (2) mixing the mixture with water, and stirring to obtain a coarse emulsion; (3) and carrying out ultrasonic emulsification on the crude emulsion to obtain the nano emulsion. The nanometer emulsion has high stability and low volatility, and can be used for treating drug-resistant bacterial infection.
Description
Technical Field
The invention relates to a nano emulsion preparation technology, in particular to a nano emulsion of plant essential oil and a preparation method and application thereof.
Background
Antibiotics have been the cornerstone of modern medicine since their discovery, and antibiotic resistance occurs when microorganisms such as bacteria, fungi, etc. develop resistance to the drugs used for treatment. The continued overuse and abuse of antibiotics in human and animal health has contributed to the emergence and spread of antibiotic resistance. Antibiotic resistance occurs around the world, and new resistance mechanisms are emerging and spreading worldwide, making more and more bacterial infections more difficult to treat, sometimes even untreatable.
Although novel antibiotics are developed, the development of these antibiotics consumes enormous manpower, financial resources, material resources and time from examination and approval to market, and the use of these antibiotics also faces the problem of drug resistance.
The plant essential oil is one of the most effective natural plant products for controlling pests, the components of the plant essential oil generally comprise micromolecules, alkenes, alcohols, aldehydes and ketones and other substances, the action mechanism is complex, pathogenic bacteria are difficult to generate resistance, and the plant essential oil has better application prospect in the fields of agriculture, forestry, biological control and the like. However, due to volatilization, photolysis, oxidation and the like of the plant essential oil, the plant essential oil is too short in retention time and too fast in degradation in the environment to exert the biological activity of the plant essential oil, so that the control effect and the lasting period of the preparation are further reduced to a great extent; but also can be rarely directly extracted and utilized because of poor stability and solubility and easy deterioration after long-term storage.
Disclosure of Invention
The invention aims to solve the problems of poor stability and solubility and over-rapid degradation of plant essential oil in the prior art, and provides the nano emulsion of the plant essential oil, the preparation method and the application thereof.
In order to achieve the above object, the present invention provides a plant essential oil nanoemulsion, comprising patchouli essential oil, an emulsifier, an encapsulant, a cross-linking agent and water, wherein the emulsifier is 1-60 parts by weight, the encapsulant is 1-20 parts by weight, the cross-linking agent is 0.1-10 parts by weight and the water is 80-1000 parts by weight, based on 10 parts by weight of patchouli essential oil.
Preferably, based on 10 parts by weight of patchouli essential oil, the content of the emulsifier is 3-10 parts by weight, the content of the encapsulant is 1-5 parts by weight, the content of the cross-linking agent is 0.1-2 parts by weight, and the content of the water is 80-150 parts by weight.
More preferably, the emulsifier is selected from at least one of Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, the encapsulating agent is selected from at least one of chitosan, gelatin, chitin, sodium alginate and gum arabic, and the cross-linking agent is thiamine pyrophosphate; the average particle size of the nano emulsion is 200-250 nm.
The second aspect of the invention provides a preparation method of a plant essential oil nano emulsion, which comprises the following steps:
(1) mixing patchouli essential oil, an emulsifier, an encapsulant and a cross-linking agent, and homogenizing to obtain a mixture;
(2) mixing the mixture obtained in the step (1) with water, and stirring to obtain a coarse emulsion;
(3) carrying out ultrasonic emulsification on the crude emulsion obtained in the step (2) to obtain the nano emulsion;
wherein the weight ratio of the patchouli essential oil to the emulsifier to the encapsulant to the cross-linking agent to the water is 1: 0.1-6: 0.1-2: 0.01-1: 8-100.
Preferably, the emulsifier is used in an amount of 3-10 parts by weight, the encapsulant is used in an amount of 1-5 parts by weight, the cross-linking agent is used in an amount of 0.1-2 parts by weight, and the water is used in an amount of 80-150 parts by weight, based on 10 parts by weight of the patchouli essential oil.
Preferably, the emulsifier is at least one selected from Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, the encapsulating agent is at least one selected from chitosan, gelatin, chitin, sodium alginate and gum arabic, and the cross-linking agent is thiamine pyrophosphate; the average particle size of the nano emulsion is 200-250 nm.
Preferably, the homogenization conditions in step (1) at least satisfy: the rotation speed is 10000-;
preferably, the conditions of the stirring in the step (2) at least satisfy: the rotation speed is 1500-.
Preferably, the conditions of the phacoemulsification in the step (3) at least satisfy: the ultrasonic amplitude transformer has specification of 4-8mm, power of 200-.
In a third aspect, the present invention provides the use of a nanoemulsion as described above and/or prepared according to the preparation method described above, for inhibiting bacteria.
Preferably, the bacteria comprise gram-negative and/or gram-positive bacteria;
preferably, the gram-negative bacteria comprise escherichia coli and the gram-positive bacteria comprise staphylococcus aureus.
Through the technical scheme, the invention has the beneficial effects that:
the nano emulsion provided by the invention is prepared by selecting patchouli essential oil with good bacteriostatic effect as a raw material and matching with an emulsifier, an encapsulant and a cross-linking agent through an ultrasonic emulsification method, and the nano emulsion prepared by the system is easy to dissolve in water, can be directly applied to drinking water and simultaneously improves the absorbability of external application. Compared with the traditional emulsion, the nano emulsion provided by the invention belongs to a thermodynamic stable system, can not be layered after hot-pressing sterilization or centrifugation, has a slow-release effect, can realize long-acting bacteriostasis, can be used as a natural antibacterial agent for treating bacterial infection resistance, and has important significance for developing medicines or products for inhibiting bacteria.
Drawings
FIG. 1 is a color observation graph of the nano emulsion prepared in example 1;
FIG. 2 is a graph of particle size analysis of the nanoemulsion obtained in example 1;
FIG. 3 is a microscopic analysis view of the nanoemulsion prepared in example 1;
FIG. 4 is a graph of particle size analysis of the nanoemulsion obtained in example 5;
FIG. 5 is a graph showing the particle size analysis of the nano emulsion prepared in comparative example 1;
FIG. 6 is a graph showing the particle size analysis of the nano emulsion prepared in comparative example 2;
fig. 7 is a bacteriostatic graph of the nano emulsion prepared in example 1.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
According to a first aspect, the invention provides a plant essential oil nanoemulsion, which comprises patchouli essential oil, an emulsifier, an encapsulant, a cross-linking agent and water, wherein the emulsifier is 1-60 parts by weight, the encapsulant is 1-20 parts by weight, the cross-linking agent is 0.1-10 parts by weight and the water is 80-1000 parts by weight based on 10 parts by weight of the patchouli essential oil.
The nano emulsion provided by the invention selects patchouli essential oil with good bacteriostatic effect as an essential oil raw material, and is matched with the emulsifier, the encapsulant and the cross-linking agent, so that the nano emulsion is easy to dissolve in water, can be directly applied to drinking water, and simultaneously improves the absorbability for external use. Compared with the traditional emulsion, the nano emulsion provided by the invention belongs to a thermodynamic stable system, can not be layered after hot-pressing sterilization or centrifugation, has a slow-release effect and can realize long-acting bacteriostasis.
According to the invention, the patchouli essential oil can be obtained commercially directly or can be prepared by the preparation method disclosed in the prior art.
According to the invention, based on 10 parts by weight of patchouli essential oil, the content of the emulsifier is 3-10 parts by weight, the content of the encapsulant is 1-5 parts by weight, the content of the cross-linking agent is 0.1-2 parts by weight, and the content of the water is 80-150 parts by weight. The inventors have found that in this preferred embodiment, it is advantageous to improve the stability and bacteriostatic effect of the nanoemulsion.
According to the invention, the emulsifier can adopt a conventional emulsifying substance, the encapsulant can also adopt a conventional substance used as a coating wall material, and the water can adopt deionized water, distilled water or water with other specifications. Preferably, the emulsifier is at least one selected from Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, the encapsulating agent is at least one selected from chitosan, gelatin, chitin, sodium alginate and gum arabic, and the water is deionized water.
According to the invention, the crosslinking agent adopts thiamine pyrophosphate, and the inventor of the invention finds that thiamine pyrophosphate can obviously improve the stability and the external absorbability of the nano emulsion prepared from patchouli essential oil in the research process.
According to the invention, the average particle size of the nano-emulsion can be set to be the conventional particle size of nano-particles, and preferably, the average particle size of the nano-emulsion is 200-250nm, and specifically can be 200nm, 210nm, 220nm, 230nm, 240nm, 250nm, or any value between the two values.
In a second aspect, the present invention provides a method for preparing a nanoemulsion of plant essential oil, comprising the following steps:
(1) mixing patchouli essential oil, an emulsifier, an encapsulant and a cross-linking agent, and homogenizing to obtain a mixture;
(2) mixing the mixture obtained in the step (1) with water, and stirring to obtain a coarse emulsion;
(3) carrying out ultrasonic emulsification on the crude emulsion obtained in the step (2) to obtain the nano emulsion;
wherein the weight ratio of the patchouli essential oil to the emulsifier to the encapsulant to the cross-linking agent to the water is 1: 0.1-6: 0.1-2: 0.01-1: 8-100.
According to the invention, the nano emulsion is prepared from the patchouli essential oil, the emulsifier, the encapsulant and the cross-linking agent by an ultrasonic emulsification method, the preparation process is simple, and the cost is saved.
According to the invention, in the preparation method, the patchouli essential oil, the emulsifier, the encapsulant and the cross-linking agent can be mixed with part of water to form a mixture, and then the mixture is mixed with the rest of water and stirred.
According to the invention, based on 10 parts by weight of patchouli essential oil, the using amount of the emulsifier is 3-10 parts by weight, the using amount of the encapsulant is 1-5 parts by weight, the using amount of the cross-linking agent is 0.1-2 parts by weight, and the using amount of the water is 80-150 parts by weight.
According to the present invention, the emulsifier may be a conventional emulsifying material, and the encapsulant may be a conventional material for coating the wall material. Preferably, the emulsifier is at least one selected from Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, and the encapsulant is at least one selected from chitosan, gelatin, chitin, sodium alginate and gum arabic.
According to the invention, the cross-linking agent is thiamine pyrophosphate to improve the stability and external absorbability of the prepared nano emulsion.
According to the invention, the average particle size of the nano emulsion is 200-250nm by controlling the process parameters of homogenization in the step (1), stirring in the step (2) and ultrasonic emulsification in the step (3).
In the present invention, any equipment and process capable of performing homogenization, such as a high-pressure homogenizer, may be used for the homogenization. Preferably, the homogenization conditions in step (1) at least satisfy: the rotation speed is 10000-; the homogenizing time is 10-15min, specifically 10min, 11min, 12min, 13min, 14min, 15min, or any value between the above two values. The inventor finds that under the preferred embodiment, the stability and the bacteriostatic effect of the nano emulsion are improved, and the production efficiency is improved.
In the present invention, any equipment and process capable of stirring, such as a magnetic stirrer, can be used for the stirring. Preferably, the stirring conditions in step (2) at least satisfy: the rotation speed is 1500-; the stirring time is 1-3min, specifically 1min, 2min, 3min, or any value between the above two values.
According to the invention, the process of mixing the mixture and water in the step (2) is as follows: and (3) dropwise adding the water into the mixture while stirring until the dropwise adding of the water is finished, and then carrying out the stirring process. Illustratively, the water dropping may be slowly dropped using a pipette gun.
In the present invention, the ultrasonic emulsification may be performed by any equipment and process capable of performing ultrasonic treatment, such as an ultrasonic cell disruptor. Preferably, the conditions of the ultrasonic emulsification in the step (3) at least satisfy: the ultrasonic amplitude transformer has a specification of 4-8mm, specifically 4mm, 5mm, 6mm, 7mm, 8mm, or any value between the two values; the power is 200-500w, and specifically can be 200w, 300w, 400w, 500w, or any value between the two values; the temperature is 0-5 deg.C, specifically 0 deg.C, 1 deg.C, 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, or any value between the above two values; the emulsifying time is 10-20min, specifically 10min, 12min, 14min, 16min, 18min, 20min, or any value between the above two values.
In a third aspect, the present invention provides the use of a nanoemulsion as described above and/or prepared according to the preparation method described above, for inhibiting bacteria.
In the present invention, the use of the nanoemulsion in inhibiting bacteria may be specifically for preparing a bacterial inhibitor, or in other conventional forms for inhibiting bacteria.
Preferably, the bacteria comprise gram-negative and/or gram-positive bacteria; illustratively, the gram-negative bacteria include escherichia coli and the gram-positive bacteria include staphylococcus aureus.
In an antibacterial test with escherichia coli (gram-negative bacteria) and staphylococcus aureus (gram-positive bacteria) as subjects, the result shows that the nanoemulsion prepared by the method provided by the invention has a better antibacterial effect than that of unencapsulated pogostemon cablin essential oil, is longer in antibacterial time and has a slow-release effect.
The present invention will be described in detail below by way of examples.
In the following examples, Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538 were purchased from American type culture Collection, patchouli essential oil was purchased from Jiangxi Hualong plant aroma Co., Ltd, and other materials were conventionally commercially available.
Example 1
(1) Mixing 8g of patchouli essential oil, 4g of Tween80, 1.5g of chitosan and 0.5g of thiamine pyrophosphate, and homogenizing for 13min by using a high-pressure homogenizer at the rotation speed of 12000rpm to obtain a mixture;
(2) dripping 86g of water into the mixture obtained in the step (1) in a stirring state by using a liquid transfer gun, and stirring for 2min by using a magnetic stirrer at the rotating speed of 2000rpm to obtain a coarse emulsion;
(3) and (3) putting the crude emulsion obtained in the step (2) into a container, and carrying out ice-water bath ultrasonic emulsification for 15min under the condition that the power is 400W by using an ultrasonic cell disruptor with an ultrasonic amplitude transformer of 6mm to obtain the nano emulsion.
Example 2
(1) Mixing 10g of patchouli essential oil, 3g of polyoxyethylene lauryl ether 58, 1g of sodium alginate and 0.1g of thiamine pyrophosphate, and homogenizing for 10min by using a high-pressure homogenizer at the rotation speed of 15000rpm to obtain a mixture;
(2) dripping 80g of water into the mixture obtained in the step (1) in a stirring state by using a liquid transfer gun, and stirring for 1min by using a magnetic stirrer at the rotating speed of 2500rpm to obtain a coarse emulsion;
(3) and (3) putting the crude emulsion obtained in the step (2) into a container, and carrying out ice-water bath ultrasonic emulsification for 10min under the condition of 500W of power by using an ultrasonic cell disrupter with an ultrasonic amplitude transformer of 4mm to obtain the nano emulsion.
Example 3
(1) Mixing 10g of patchouli essential oil, 5g of lecithin, 5g of polyvinyl alcohol, 5g of chitin and 2g of thiamine pyrophosphate, and homogenizing for 15min by using a high-pressure homogenizer at the rotation speed of 10000rpm to obtain a mixture;
(2) dropwise adding 150g of water into the mixture obtained in the step (1) in a stirring state by using a liquid transfer gun, and stirring for 3min by using a magnetic stirrer at the rotating speed of 1500rpm to obtain a coarse emulsion;
(3) and (3) putting the crude emulsion obtained in the step (2) into a container, and carrying out ice-water bath ultrasonic emulsification for 20min under the condition of 200W of power by using an ultrasonic cell disrupter with an ultrasonic amplitude transformer of 8mm to obtain the nano emulsion.
Example 4
(1) Mixing 10g of patchouli essential oil, 60g of Tween80, 10g of Arabic gum, 10g of gelatin and 10g of thiamine pyrophosphate, and homogenizing for 13min by using a high-pressure homogenizer at the rotation speed of 12000rpm to obtain a mixture;
(2) dropwise adding 1000g of water into the mixture obtained in the step (1) in a stirring state by using a liquid transfer gun, and stirring for 3min by using a magnetic stirrer at the rotating speed of 1500rpm to obtain a coarse emulsion;
(3) and (3) putting the crude emulsion obtained in the step (2) into a container, and carrying out ice-water bath ultrasonic emulsification for 20min under the condition of 200W of power by using an ultrasonic cell disrupter with an ultrasonic amplitude transformer of 8mm to obtain the nano emulsion.
Example 5
A nanoemulsion was prepared according to the procedure of example 3, except that the crosslinker thiamine pyrophosphate was replaced with N, N-methylenebisacrylamide.
Comparative example 1
(1) Mixing 10g of patchouli essential oil, 5g of lecithin, 5g of polyvinyl alcohol and 5g of chitin, and homogenizing for 15min by using a high-pressure homogenizer at the rotation speed of 10000rpm to obtain a mixture;
(2) dropwise adding 150g of water into the mixture obtained in the step (1) in a stirring state by using a liquid transfer gun, and stirring for 3min by using a magnetic stirrer at the rotating speed of 1500rpm to obtain a coarse emulsion;
(3) and (3) putting the crude emulsion obtained in the step (2) into a container, and carrying out ice-water bath ultrasonic emulsification for 20min under the condition of 200W of power by using an ultrasonic cell disrupter with an ultrasonic amplitude transformer of 8mm to obtain the nano emulsion.
Comparative example 2
(1) Mixing 10g of patchouli essential oil and 5g of nonionic surfactant (tween20) at room temperature, and then adding 2g of co-surfactant (propylene glycol), 5g of emulsifier (lecithin) and 100g of water under mild stirring to prepare a crude emulsion;
(2) and (3) finely homogenizing the crude emulsion obtained in the step (1) for 20min by using a homogenizer at the rotating speed of 8000rpm to obtain the nano emulsion.
Test example 1
Color observation is carried out on the patchouli essential oil and the nano emulsion prepared in the example 1, and as shown in figure 1, the nano emulsion prepared in the example 1 is milky and uniform milky emulsion; the mean particle size of the nano emulsion obtained in example 1 was measured by a Malvern particle size analyzer, and the analysis result of the particle size is shown in fig. 2, and the mean particle size of the nano emulsion obtained in example 1 was 236nm and had no impurity peak. The nano emulsion prepared in example 1 has uniform liquid distribution and uniform size through microscope observation, and the result of microscope analysis is shown in fig. 3.
When the nano-emulsions prepared in example 5, comparative example 1 and comparative example 2 were subjected to particle size analysis, respectively, as shown in fig. 4 to 6, it was found that the nano-emulsions prepared in example 5, comparative example 1 and comparative example 2 had an insufficiently uniform particle size distribution and a hetero-peak, and accordingly, the nano-emulsions prepared in example 5, comparative example 1 and comparative example 2 had some instability.
The nano emulsions prepared in examples 1 to 5 are all easily soluble in water, can be directly applied to drinking water, belong to a thermodynamically stable system, and do not delaminate after autoclaving or centrifugation.
Test example 2 detection of drug resistance of clinically isolated strains
The sensitivity of clinically isolated E.coli (gram negative bacteria) and S.aureus (gram positive bacteria) to the nanoemulsion prepared in example 1, as well as to the essential oil of Pogostemon cablin, was determined by the broth microdilution method, with reference to the Standards issued by the Clinical and Laboratory Standards Institute (CLSI). Coli (E.coli) ATCC 8739 and Staphylococcus aureus (S.aureus) ATCC 6538 were used as the quality control strains in this test, and all the antibiotics and the culture medium were in the quality control range. The test results show that the nanoemulsion prepared in example 1 shows better bacteriostatic effect and longer bacteriostatic time than the unencapsulated pogostemon cablin essential oil.
Test example 3 measurement of bacteriostatic Activity of nanoemulsion
The bacteriostatic activity of the nanoemulsion prepared in example 1 and the essential oil of patchouli was measured by the dilution coating plate method with reference to the standards published by CLSI, and the result showed that the nanoemulsion prepared in example 1 exhibited better bacteriostatic activity than the unencapsulated essential oil of patchouli.
Test example 4 Effect of nanoemulsion on bacterial growth
The effect of the nanoemulsion prepared in example 1 on the growth of bacteria was analyzed by a paper diffusion method using escherichia coli (e.coli) ATCC 8739 and staphylococcus aureus (s.aureus) ATCC 6538 as test bacteria.
The specific analysis process is as follows: escherichia coli (E.coli) ATCC 8739 and Staphylococcus aureus (S.aureus) ATCC 6538 were cultured, respectively, so that the concentration of the corresponding bacteria in LB liquid medium became 107CFU/mL, and then respectively transferring to LB agar plates; mu.L of the nanoemulsion obtained in example 1 was transferred to a pipetteSpotting on a filter paper sheet with a diameter of 6mm, placing the filter paper sheet on LB agar plates inoculated with two kinds of bacteria, respectively, culturing at a temperature of (37 + -1) ° C, and measuring and recording the diameter (DD) of the zone of inhibition at a fixed point every day, wherein the graph of the diameter (DD) of the zone of inhibition of the two kinds of bacteria as a function of the culturing time is shown in FIG. 7.
From the results of the paper diffusion method, it can be seen that the nanoemulsion prepared in example 1 has a very strong bacteriostatic ability, and escherichia coli ATCC 8739 in the test bacteria is most sensitive to the nanoemulsion prepared in example 1, and the diameter of the zone of inhibition is the largest (17.15 mm); the nanoemulsion prepared in example 1 had inhibition zones with diameters ranging from 7 to 11mm and from 10 to 17mm for Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739, respectively. The result shows that the nanoemulsion prepared in example 1 has stronger capability of inhibiting the growth of escherichia coli ATCC 8739, and the combined antibacterial effect of the nanoemulsion prepared in example 1 and nisin is better than that of single nisin, so that the nanoemulsion prepared in example 1 has a certain slow-release effect.
The diameters of the inhibition zones of the nano-emulsions prepared in example 5, comparative example 1 and comparative example 2 against two kinds of bacteria were analyzed by the same paper diffusion method using escherichia coli (e.coli) ATCC 8739 and staphylococcus aureus (s.aureus) ATCC 6538 as test bacteria, and the test results were: the diameters of inhibition zones of the nano-emulsions prepared in example 5, comparative example 1 and comparative example 2 on staphylococcus aureus ATCC 6538 are respectively 6-10mm, 6-7mm and 6-9 mm; the diameters of inhibition zones of the nanoemulsions prepared in example 5, comparative example 1 and comparative example 2 against E.coli ATCC 8739 ranged from 9 to 15mm, 8 to 13mm and 9 to 14mm, respectively.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. The nanoemulsion of the plant essential oil is characterized by comprising patchouli essential oil, an emulsifier, an encapsulant, a cross-linking agent and water, wherein the emulsifier is 1-60 parts by weight, the encapsulant is 1-20 parts by weight, the cross-linking agent is 0.1-10 parts by weight and the water is 80-1000 parts by weight based on 10 parts by weight of the patchouli essential oil.
2. The nanoemulsion of claim 1, wherein the emulsifier is contained in an amount of 3-10 parts by weight, the encapsulant is contained in an amount of 1-5 parts by weight, the cross-linking agent is contained in an amount of 0.1-2 parts by weight, and the water is contained in an amount of 80-150 parts by weight, based on 10 parts by weight of patchouli essential oil.
3. The nanoemulsion of claim 1 or 2, wherein the emulsifier is selected from at least one of Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, the encapsulating agent is selected from at least one of chitosan, gelatin, chitin, sodium alginate and gum arabic, and the cross-linking agent is thiamine pyrophosphate;
the average particle size of the nano emulsion is 200-250 nm.
4. The preparation method of the plant essential oil nanoemulsion is characterized by comprising the following steps of:
(1) mixing patchouli essential oil, an emulsifier, an encapsulant and a cross-linking agent, and homogenizing to obtain a mixture;
(2) mixing the mixture obtained in the step (1) with water, and stirring to obtain a coarse emulsion;
(3) carrying out ultrasonic emulsification on the crude emulsion obtained in the step (2) to obtain the nano emulsion;
wherein the weight ratio of the patchouli essential oil to the emulsifier to the encapsulant to the cross-linking agent to the water is 1: 0.1-6: 0.1-2: 0.01-1: 8-100.
5. The preparation method according to claim 4, wherein the emulsifier is used in an amount of 3 to 10 parts by weight, the encapsulant is used in an amount of 1 to 5 parts by weight, the crosslinking agent is used in an amount of 0.1 to 2 parts by weight, and the water is used in an amount of 80 to 150 parts by weight, based on 10 parts by weight of the patchouli essential oil.
6. The preparation method according to claim 4, wherein the emulsifier is at least one selected from Tween80, lecithin, polyoxyethylene lauryl ether 58 and polyvinyl alcohol, the encapsulating agent is at least one selected from chitosan, gelatin, chitin, sodium alginate and gum arabic, and the cross-linking agent is thiamine pyrophosphate;
the average particle size of the nano emulsion is 200-250 nm.
7. The production method according to any one of claims 4 to 6, wherein the condition for homogenization in step (1) at least satisfies: the rotation speed is 10000-;
preferably, the conditions of the stirring in the step (2) at least satisfy: the rotation speed is 1500-.
8. The production method according to any one of claims 4 to 6, wherein the condition of the ultrasonic emulsification in the step (3) satisfies at least: the ultrasonic amplitude transformer has specification of 4-8mm, power of 200-.
9. Use of a nanoemulsion according to any one of claims 1 to 3 and/or of a nanoemulsion obtained by the preparation method according to any one of claims 4 to 8 for inhibiting bacteria.
10. Use according to claim 9, wherein the bacteria comprise gram-negative and/or gram-positive bacteria;
preferably, the gram-negative bacteria comprise escherichia coli and the gram-positive bacteria comprise staphylococcus aureus.
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| CN113827563A (en) * | 2021-11-02 | 2021-12-24 | 澳门大学 | Nanoemulsion preparation for treating migraine and preparation method and application thereof |
| CN114426905A (en) * | 2021-12-09 | 2022-05-03 | 南京师范大学 | Essential oil composite nano emulsion and preparation method thereof |
| CN114426905B (en) * | 2021-12-09 | 2024-02-06 | 南京师范大学 | Essential oil composite nano emulsion and preparation method thereof |
| CN114989889A (en) * | 2022-05-18 | 2022-09-02 | 广东本研新材料科技有限公司 | Preparation method of plant essential oil microspheres |
| CN115736007A (en) * | 2022-12-05 | 2023-03-07 | 湖南省林业科学院 | Litsea cubeba essential oil nanoparticles, preparation method thereof and application thereof in pepper preservation |
| CN116350678A (en) * | 2023-05-18 | 2023-06-30 | 南京中医药大学 | Nanoemulsion for treating psoriasis dermatitis and preparation method and application thereof |
| CN116350678B (en) * | 2023-05-18 | 2024-07-26 | 南京中医药大学 | Nanoemulsion for treating psoriasis dermatitis and preparation method and application thereof |
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