CN113433116A - Stainless steel ultrasonic sheet chemiluminescence solution detection device and application method thereof - Google Patents
Stainless steel ultrasonic sheet chemiluminescence solution detection device and application method thereof Download PDFInfo
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- CN113433116A CN113433116A CN202110713845.5A CN202110713845A CN113433116A CN 113433116 A CN113433116 A CN 113433116A CN 202110713845 A CN202110713845 A CN 202110713845A CN 113433116 A CN113433116 A CN 113433116A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The invention provides a chemiluminescence solution detection device which comprises a non-porous stainless steel atomization sheet and a chemiluminescence solution storage tank arranged on the back of the non-porous stainless steel atomization sheet. On the basis of a piezoelectric ultrasonic transducer of a microporous stainless steel atomization sheet, the ultrasonic chemiluminescence is generated by using a nonporous stainless steel sheet to replace a porous microporous stainless steel sheet and adding a chemiluminescence solution. The invention can avoid the leakage of the chemiluminescent solution through the micropores, effectively inhibit the atomization of the chemiluminescent solution and is not beneficial to the chemiluminescent detection, thereby realizing the longer-time ultrasonic chemiluminescent detection. Meanwhile, the ultrasonic device can obviously enhance luminescence, so that solutions which do not produce chemiluminescence or produce weak chemiluminescence can produce strong light without ultrasonic, and the solution can be observed by human eyes, smart phones or related instruments. The chemiluminescence solution detection device has the advantages of low manufacturing cost, low driving voltage, small volume and convenient use.
Description
Technical Field
The invention belongs to the technical field of chemiluminescence solution detection, relates to a chemiluminescence solution detection device and a using method thereof, and particularly relates to a stainless steel ultrasonic sheet chemiluminescence solution detection device and a using method thereof.
Background
Sonochemiluminescence (SCL) refers to the emission of light produced by the chemical reaction of sonosome products produced by cavitation bubbles. Sonochemiluminescence has some unique advantages over Chemiluminescence (CL) and Electrochemiluminescence (ECL). In a typical sonochemiluminescence system, active oxygen can be generated in situ by ultrasound without the addition of additional ancillary reagents. Thus, sonochemiluminescence is receiving increasing attention.
To date, some types of ultrasound equipment have been used for sonochemiluminescence studies. One is a horn piezoelectric ultrasonic transducer with a titanium probe. One is a Langmuir-type ultrasonic transducer driven by a function generator and power amplifier. However, both of these devices are expensive, bulky, and consume high power, greatly limiting their widespread use in sonochemiluminescence.
Therefore, how to find a more suitable ultrasonic chemiluminescence device for detecting a chemiluminescence solution and the like, which solves the above defects of the existing device and is more convenient for application and popularization, has become one of the focuses of great attention of many researchers in the field.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a chemiluminescence solution detection apparatus and a method for using the same, in particular to a chemiluminescence solution detection apparatus for a stainless steel ultrasonic sheet. The chemiluminescence solution detection device provided by the invention has the advantages of low manufacturing cost, low driving voltage, small volume, easy control and convenient use, can realize long-time ultrasonic chemiluminescence detection, and is beneficial to realizing industrial scale production and application.
The invention provides a chemiluminescence solution detection device which comprises a non-porous stainless steel atomization sheet and a chemiluminescence solution storage tank arranged on the back of the non-porous stainless steel atomization sheet.
Preferably, the nonporous stainless steel atomization sheet is a modified microporous stainless steel atomization sheet;
the improvement specifically comprises replacing a microporous stainless steel sheet in the stainless steel atomization sheet with a nonporous stainless steel sheet.
Preferably, the stainless steel atomization plate comprises an ultrasonic atomization plate;
the stainless steel atomization sheet comprises a microporous stainless steel atomization sheet for a humidifier;
the humidifier includes a home humidifier or a mini humidifier.
Preferably, the diameter of the nonporous stainless steel atomization sheet is 10-50 mm;
the front surface of the nonporous stainless steel atomization sheet is a surface provided with a piezoelectric ceramic ring;
the chemiluminescent solution storage tank is formed by a rear panel of a non-porous stainless steel atomization sheet and an annular insulating device compounded on the rear panel.
Preferably, the height of the annular insulating device is 0.2-0.7 mm;
the diameter of the liquid storage tank is 50-70% of that of the stainless steel atomization piece without the hole;
the annular insulating device is made of one or more of resin, foam, plastic, rubber and ceramic.
Preferably, the chemiluminescent solution detecting means further comprises the power supply means;
the power supply device is connected with the nonporous stainless steel atomization sheet through a lead;
the output voltage of the power supply device is 5V;
the output current of the power supply device is 300 mA.
The invention provides a using method of a chemiluminescence solution detection device, which comprises the following steps:
and placing the chemiluminescent solution to be detected in a chemiluminescent solution storage tank of a detection device, and starting ultrasonic vibration in a darkroom environment to trigger ultrasonic chemiluminescence for detection.
Preferably, the chemiluminescence solution to be detected comprises a solution which does not produce chemiluminescence or produces weak chemiluminescence in the absence of ultrasound;
the sonochemiluminescence includes sonochemiluminescence visible to the naked eye or observable by an instrument.
Preferably, the chemiluminescence solution to be detected comprises one or more of a luminol solution, a hydrogen peroxide-luminol solution and a glucose-glucose oxidase-luminol pretreatment solution;
the molar concentration of the chemiluminescence solution to be detected is 0.1-50 mu M.
Preferably, the intensity of the ultrasonic chemiluminescence has a correlation with the molar concentration of the chemiluminescence solution to be detected;
the detection comprises qualitative detection and/or quantitative detection.
The invention provides a chemiluminescence solution detection device which comprises a non-porous stainless steel atomization sheet and a chemiluminescence solution storage tank arranged on the back of the non-porous stainless steel atomization sheet. Compared with the prior art, the piezoelectric ultrasonic transducer of the microporous stainless steel atomization sheet is specially selected, creative improvement is carried out, and ultrasonic chemiluminescence is generated by using a nonporous stainless steel sheet to replace a porous microporous stainless steel sheet and adding a chemiluminescence solution. The invention can avoid the leakage of the chemiluminescent solution through the micropores, effectively inhibit the atomization of the chemiluminescent solution and is not beneficial to the chemiluminescent detection, thereby realizing the longer-time ultrasonic chemiluminescent detection. Meanwhile, the ultrasonic device can obviously enhance luminescence, so that solutions which do not produce chemiluminescence or produce weak chemiluminescence can produce strong light without ultrasonic, and the solution can be observed easily by human eyes, smart phones or related instruments.
On the basis of a microporous stainless steel atomization sheet used by a household USB humidifier and the like, a nonporous stainless steel ultrasonic sheet is manufactured by replacing a porous stainless steel sheet on the stainless steel microporous atomization sheet with a nonporous stainless steel sheet (common stainless steel sheet), a chemiluminescent solution is added on the stainless steel sheet, and then ultrasonic chemiluminescence is caused by ultrasonic vibration of the stainless steel ultrasonic sheet. The improved nonporous stainless steel ultrasonic transducer based on the microporous stainless steel atomizing sheet has the advantages of low manufacturing cost, low driving voltage, small volume and convenient use, and the piezoelectric ultrasonic transducer of the microporous stainless steel atomizing sheet is widely used for household humidifiers and the like,
experimental results show that the detection device provided by the invention realizes the quantitative detection of hydrogen peroxide within the range of 0.5-50 mu M, H2O2Concentration and SCL intensity are linearAnd the simple, quick and efficient instant detection is realized through the correlation of the intensity and the concentration.
Drawings
FIG. 1 is an external view of a semi-finished product of a chemiluminescent solution detection device for a stainless steel ultrasonic sheet prepared by the invention;
FIG. 2 is a back view of the chemiluminescent solution detection device of the stainless steel ultrasonic plate prepared in the present invention;
FIG. 3 is a plot of luminol concentration (. mu.M) versus sonochemiluminescence intensity (SCLintensity) in example 1 of the present invention;
FIG. 4 is a graph of the pH versus the intensity of sonochemiluminescence (SCLs intensity) of CBS buffer in example 2 of the present invention;
FIG. 5 is a plot of hydrogen peroxide concentration versus sonochemiluminescence intensity (SCLintensity) for example 3 of the present invention.
Detailed Description
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the invention, and not to limit the scope of the claims.
All of the starting materials of the present invention, without particular limitation as to their source, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art.
All the raw materials of the present invention are not particularly limited in their purity, and the present invention preferably employs purity requirements that are conventional in the field of analytical purification or chemiluminescent solution detection.
All the raw materials, the marks and the acronyms thereof belong to the conventional marks and acronyms in the field, each mark and acronym is clear and definite in the field of related application, and the raw materials can be purchased from the market or prepared by a conventional method by the technical staff in the field according to the marks, the acronyms and the corresponding application.
The invention provides a chemiluminescence solution detection device which comprises a non-porous stainless steel atomization sheet and a chemiluminescence solution storage tank arranged on the back of the non-porous stainless steel atomization sheet.
In the present invention, the non-porous stainless steel atomizing sheet is preferably a modified microporous stainless steel atomizing sheet. Wherein, the improvement specifically comprises replacing a microporous stainless steel sheet in the stainless steel atomization sheet with a nonporous stainless steel sheet.
In the present invention, the stainless steel atomization plate preferably comprises an ultrasonic atomization plate. Also known as a micro-porous stainless steel atomizing sheet type piezoelectric ultrasonic transducer.
In the present invention, the stainless steel atomization sheet preferably includes a microporous stainless steel atomization sheet for a humidifier, and more particularly, the humidifier preferably includes a home humidifier or a mini humidifier.
In the invention, the diameter of the nonporous stainless steel atomization sheet is preferably 10-50 mm, more preferably 15-45 mm, more preferably 20-40 mm, and more preferably 25-35 mm.
In the invention, the front surface of the nonporous stainless steel atomization sheet is preferably the surface provided with the piezoelectric ceramic ring.
In the present invention, the chemiluminescent solution reservoir is preferably a reservoir which can store liquid and is formed by a back plate of a non-porous stainless steel atomizing sheet and an annular insulating device compounded on the back plate.
In the invention, the height of the annular insulating device is preferably 0.2-0.7 mm, more preferably 0.3-0.6 mm, and more preferably 0.4-0.5 mm.
In the present invention, the diameter of the reservoir is preferably 50% to 70%, more preferably 53% to 68%, more preferably 55% to 65%, and more preferably 58% to 63% of the diameter of the stainless steel atomizing plate without holes.
In the present invention, the material of the annular insulating device preferably includes one or more of resin, foam, plastic, rubber and ceramic, and more preferably, the material of the annular insulating device is resin, foam, plastic, rubber or ceramic.
In the present invention, the chemiluminescent solution detection means preferably includes the power supply means.
In the present invention, the power supply means and the non-porous stainless steel atomizing plate are preferably connected by a wire.
In the present invention, the output voltage of the power supply device is preferably 5V.
In the present invention, the output current of the power supply device is preferably 300 mA.
The invention provides a chemiluminescence solution detection device for a stainless steel ultrasonic sheet. On the basis of a microporous stainless steel atomization sheet of a household USB humidifier, a nonporous stainless steel ultrasonic sheet is manufactured by replacing a porous stainless steel sheet on the microporous stainless steel atomization sheet with a nonporous stainless steel sheet.
Referring to fig. 1, fig. 1 is an appearance view of a semi-finished product of a chemiluminescence solution detection device for a stainless steel ultrasonic sheet prepared by the invention. The upper diagram in fig. 1 is a piezoelectric ceramic ring surface, i.e., a front surface, of the chemiluminescent solution detection device. The lower diagram is the non-pressure electroceramic ring face, i.e. the back face, of the chemiluminescent solution detection device.
Referring to fig. 2, fig. 2 is a back appearance view of the chemiluminescence solution detection apparatus for a stainless steel ultrasonic sheet prepared according to the present invention. As can be seen from fig. 2, the piezoelectric ceramic ring is faced with an annular plastic insulating sheet.
According to the invention, a ring-shaped plastic sheet with a specific size is stuck on the surface of a stainless steel sheet without a piezoelectric ceramic ring to form a groove (as shown in figure 2), a chemiluminescent solution (such as a luminol solution) is added into the groove, and then ultrasonic chemiluminescence is caused by ultrasonic vibration of a stainless steel ultrasonic sheet.
According to the invention, the nonporous stainless steel sheet is used for replacing the porous stainless steel sheet, so that the chemical luminescent solution can be prevented from leaking through micropores, the atomization of the chemical luminescent solution can be effectively inhibited, and the long-time ultrasonic chemical luminescent detection can be realized. Meanwhile, the ultrasonic device can obviously enhance luminescence, so that solutions which do not produce chemiluminescence or produce weak chemiluminescence can produce strong light without ultrasonic, and the solution can be observed easily through a mobile phone, human eyes or corresponding instruments.
The invention provides a using method of a chemiluminescence solution detection device, which comprises the following steps:
and placing the chemiluminescent solution to be detected in a chemiluminescent solution storage tank of a detection device, and starting ultrasonic vibration in a darkroom environment to trigger ultrasonic chemiluminescence for detection.
In the present invention, the chemiluminescent solution to be detected preferably includes a solution that does not produce chemiluminescence or produces very weak chemiluminescence in the absence of ultrasound.
In the present invention, the sonochemiluminescence preferably comprises sonochemiluminescence visible to the naked eye or observable with an instrument.
In the invention, the to-be-detected chemiluminescence solution preferably comprises one or more of a luminol solution, a hydrogen peroxide-luminol solution and a glucose-glucose oxidase-luminol pretreatment solution, and more preferably comprises the luminol solution, the hydrogen peroxide-luminol solution or the glucose-glucose oxidase-luminol pretreatment solution.
In the invention, the molar concentration of the chemiluminescence solution to be detected is preferably 0.1-50 μ M, more preferably 0.5-40 μ M, more preferably 1-30 μ M, and more preferably 5-25 μ M.
In the present invention, the intensity of the ultrasonic chemiluminescence is preferably correlated with the molar concentration of the chemiluminescent solution to be measured. Specifically, the molar concentration of the chemiluminescent solution to be measured in the present invention may exceed the molar concentration described above, but in the present invention, the intensity of the ultrasonic chemiluminescence is preferably linearly changed from the molar concentration of the chemiluminescent solution to be measured within the molar concentration range described above. When the molar concentration is higher than the above range, the degree of change in the intensity of the sonochemiluminescence decreases.
In the present invention, the detection preferably comprises a qualitative detection and/or a quantitative detection, more preferably a qualitative detection or a quantitative detection.
According to the invention, 250 mu M luminol solution is added into the groove of the ultrasonic sheet with the special structure provided in the above steps, and an ultrasonic chemiluminescence test is carried out. By electrifying the stainless steel ultrasonic sheet driving circuit board, the stainless steel ultrasonic sheet can promote the generation of active oxygen, thereby triggering sonochemiluminescence. The light signal generated after the power-on is photographed and recorded by using the smart phone in the dark box, and the strong luminous phenomenon of luminol in the device can be observed.
The invention prepares the mixed solution with the luminol concentration of 250 mu M and the hydrogen peroxide concentration of 0.1 mu M-100 mM to form a certain gradient, repeats the steps, and uses the smart phone to take a picture and record, so that the chemiluminescence intensity can be observed to be obviously enhanced along with the increase of the hydrogen peroxide concentration. And obtaining a relation curve of the luminous intensity and the hydrogen peroxide concentration through subsequent data processing, and realizing the quantitative detection of the hydrogen peroxide concentration.
The invention provides a stainless steel ultrasonic sheet chemiluminescence solution detection device and a using method thereof. The invention particularly selects a piezoelectric ultrasonic transducer of a microporous stainless steel atomization sheet, and generates ultrasonic chemiluminescence by using a nonporous stainless steel sheet to replace a porous microporous stainless steel sheet and adding a chemiluminescent solution. The invention can avoid the leakage of the chemiluminescent solution through the micropores, effectively inhibit the atomization of the chemiluminescent solution and is not beneficial to the chemiluminescent detection, thereby realizing the longer-time ultrasonic chemiluminescent detection. Meanwhile, the ultrasonic device can obviously enhance luminescence, so that solutions which do not produce chemiluminescence or produce weak chemiluminescence can produce strong light without ultrasonic, and the solution can be observed easily by human eyes, smart phones or related instruments.
On the basis of a microporous stainless steel atomization sheet used by a household USB humidifier and the like, a nonporous stainless steel ultrasonic sheet is manufactured by replacing a porous stainless steel sheet on the stainless steel microporous atomization sheet with a nonporous stainless steel sheet (common stainless steel sheet), a chemiluminescent solution is added on the stainless steel sheet, and then ultrasonic chemiluminescence is caused by ultrasonic vibration of the stainless steel ultrasonic sheet. The nonporous stainless steel ultrasonic sheet chemiluminescence device provided by the invention has the advantages of low manufacturing cost, low driving voltage, safety, small volume, portability and convenient use.
Experimental results show that the detection device provided by the invention realizes quantitative detection of hydrogen peroxide within the range of 0.5-50 mu M through early-stage experimental condition optimization, and H is2O2Concentration is linearly related to SCL intensityThe method realizes simple, quick and efficient instant detection through the correlation of the intensity and the concentration. According to the catalytic reaction of the glucose, the invention can realize the quantitative detection of the glucose within the concentration range of 0.5-70 mu M.
To further illustrate the present invention, a chemiluminescent solution detection device and its method of use will be described in detail with reference to the following examples, but it should be understood that these examples are carried out on the premise of the technical solution of the present invention, and the detailed embodiments and specific operation procedures are given, only for further illustrating the features and advantages of the present invention, not for limiting the claims of the present invention, and the scope of the present invention is not limited to the following examples.
Example 1
Chemiluminescence solution detection device for preparing stainless steel ultrasonic sheet
On the basis of a microporous stainless steel atomization sheet of a household USB humidifier, a porous stainless steel sheet on the microporous stainless steel atomization sheet is changed into a non-porous stainless steel sheet, and an annular plastic insulation sheet is attached to the surface of a non-pressure electric ceramic ring to form a groove with the diameter of 10 mm.
And then adding chemiluminescence solutions (a luminol solution with a certain gradient in the range of 0.5-200 mu M, the luminol solution and a 25 mu M hydrogen peroxide solution) into the grooves respectively, wherein the adding amount is generally 30-50 mu L, and then, ultrasonic chemiluminescence is caused by ultrasonic vibration of a stainless steel ultrasonic sheet.
The ultrasonic chemiluminescence process of a chemiluminescent solution detected by the chemiluminescent solution detection device provided in embodiment 1 of the invention was analyzed.
Referring to fig. 3, fig. 3 is a graph of luminol concentration (μ M) versus sonochemiluminescence intensity (SCLIntensity) in example 1 of the present invention.
As can be seen from fig. 3, in c (luminel) 100 μ M [ other experimental conditions: CBS buffer solution (pH 12), the experimental conditions can be selected according to the results of the optimization experiment ], the increase of the luminescence intensity gradually becomes slow, and 100 μ M is selected for the concentration of the luminol solution in the subsequent detection of the concentrations of hydrogen peroxide and glucose (example 1 is mainly the optimization process of the experimental conditions in the actual detection).
Example 2
pH of CBS buffer vs. intensity of sonochemiluminescence (SCLintensity).
pH Condition optimization examples
Detecting solutions with different pH values (the pH gradient is 9.4, 10, 10.5, 11, 11.5, 12, 12.5 and 13) are prepared, mixed solutions are added into reaction grooves on the reverse side of the invention, and ultrasonic chemiluminescence is caused by ultrasonic action.
Referring to FIG. 4, FIG. 4 is a graph of pH vs. sonochemiluminescence intensity (SCLintensity) of CBS buffer in example 2 of the present invention.
When the pH of CBS buffer was 12 [ other experimental conditions: c (lumineol) 100. mu.M, c (H)2O2)＝25μM]When the concentration of hydrogen peroxide and glucose is detected, the pH value of the CBS buffer solution can be selected to be 12.
Example 3
Relationship of Hydrogen peroxide concentration to SCL Strength
When the pH of CBS buffer was 12 [ other experimental conditions: when the concentration of c (lumineol) reaches 100 μ M, the luminous intensity reaches the maximum value, the pH intensity is decreased while the pH intensity is increased, and the pH value of the CBS buffer is selected to be 12 when the concentrations of hydrogen peroxide and glucose are measured subsequently.
Preparing a mixed solution of luminol and hydrogen peroxide, wherein the concentration of the luminol is 100 mu M and H2O2The concentration is between 0.5-50 μ M, and the quantitative detection of the hydrogen peroxide is carried out.
The mixed solution is added into a reaction groove on the reverse side of the atomization sheet, and then ultrasonic chemiluminescence is caused by ultrasonic vibration of the stainless steel ultrasonic sheet.
The ultrasonic chemiluminescence process of the chemiluminescence solution detected by the chemiluminescence solution detection device provided in embodiment 3 of the invention is analyzed.
Referring to fig. 5, fig. 5 is a graph of hydrogen peroxide concentration versus sonochemiluminescence intensity (SCLIntensity) for example 3 of the present invention.
As can be seen from FIG. 5, when the concentration of the hydrogen peroxide solution is between 0.5 and 50 μ M, a linear relationship between the hydrogen peroxide concentration and the SCL intensity is obtained, and the quantitative detection of hydrogen peroxide can be realized according to the linear relationship.
While the present invention has been described in detail with respect to a stainless steel ultrasonic sheet chemiluminescent solution detection device and method of use thereof, the present invention is described in its broadest possible embodiment and is thus intended to be used in an understanding of the principles and spirit of the present invention, including the best mode and so forth, and to enable any person skilled in the art to make and use the present invention, including making and using any devices or systems and performing any incorporated methods. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention. The scope of the invention is defined by the claims and may include other embodiments that occur to those skilled in the art. Such other embodiments are intended to be within the scope of the claims if they have structural elements that do not differ from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal languages of the claims.
Claims (10)
1. The chemiluminescence solution detection device is characterized by comprising a non-porous stainless steel atomization sheet and a chemiluminescence solution storage tank arranged on the back of the non-porous stainless steel atomization sheet.
2. The chemiluminescent solution detection device according to claim 1 wherein the non-porous stainless steel atomizing sheet is a modified microporous stainless steel atomizing sheet;
the improvement specifically comprises replacing a microporous stainless steel sheet in the stainless steel atomization sheet with a nonporous stainless steel sheet.
3. The chemiluminescent solution detection device according to claim 1 wherein the stainless steel atomizing sheet comprises an ultrasonic atomizing sheet;
the stainless steel atomization sheet comprises a microporous stainless steel atomization sheet for a humidifier;
the humidifier includes a home humidifier or a mini humidifier.
4. The chemiluminescent solution detection device according to claim 1 wherein the diameter of the non-porous stainless steel atomizing sheet is 10 to 50 mm;
the front surface of the nonporous stainless steel atomization sheet is a surface provided with a piezoelectric ceramic ring;
the chemiluminescent solution storage tank is formed by a rear panel of a non-porous stainless steel atomization sheet and an annular insulating device compounded on the rear panel.
5. The chemiluminescent solution detection device according to claim 4 wherein the height of the annular insulating means is 0.2 to 0.7 mm;
the diameter of the liquid storage tank is 50-70% of that of the stainless steel atomization piece without the hole;
the annular insulating device is made of one or more of resin, foam, plastic, rubber and ceramic.
6. The chemiluminescent solution detection device according to claim 1 further comprising the power supply;
the power supply device is connected with the nonporous stainless steel atomization sheet through a lead;
the output voltage of the power supply device is 5V;
the output current of the power supply device is 300 mA.
7. The use method of the chemiluminescence solution detection device is characterized by comprising the following steps:
and placing the chemiluminescent solution to be detected in a chemiluminescent solution storage tank of a detection device, and starting ultrasonic vibration in a darkroom environment to trigger ultrasonic chemiluminescence for detection.
8. The use method according to claim 7, wherein the chemiluminescence solution to be detected comprises a solution which does not produce chemiluminescence or produces very weak chemiluminescence in the absence of ultrasound;
the sonochemiluminescence includes sonochemiluminescence visible to the naked eye or observable by an instrument.
9. The use method according to claim 7, wherein the chemiluminescent solution to be tested comprises one or more of a luminol solution, a hydrogen peroxide-luminol solution and a glucose-glucose oxidase-luminol pretreatment solution;
the molar concentration of the chemiluminescence solution to be detected is 0.1-50 mu M.
10. The use method according to claim 1, wherein the intensity of the ultrasonic chemiluminescence has a correlation with the molar concentration of the chemiluminescence solution to be measured;
the detection comprises qualitative detection and/or quantitative detection.
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