CN113433102A - Fluorescent carbon dot and fluorescence kit for detecting ciprofloxacin prepared by same - Google Patents
Fluorescent carbon dot and fluorescence kit for detecting ciprofloxacin prepared by same Download PDFInfo
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- CN113433102A CN113433102A CN202110641993.0A CN202110641993A CN113433102A CN 113433102 A CN113433102 A CN 113433102A CN 202110641993 A CN202110641993 A CN 202110641993A CN 113433102 A CN113433102 A CN 113433102A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
The invention provides a fluorescent carbon dot and a fluorescence kit for detecting ciprofloxacin prepared by the same, the method is characterized in that the kit is prepared simply and comprises a solution A and a solution B, wherein the solution A is a prepared lightless carbon dot and is prepared by reacting a naphthalene derivative with a dopamine analogue, the solution B is a copper sulfate solution, the solution A and the solution B are mixed and then added with ciprofloxacin to test a fluorescence spectrum, the ciprofloxacin content in a sample can be calculated according to an established working curve, and the rapid detection of the ciprofloxacin can be realized.
Description
Technical Field
The invention relates to the field of drug detection, in particular to a fluorescent carbon dot and a fluorescence kit for detecting ciprofloxacin, which is prepared from the fluorescent carbon dot.
Background
The detection of drug residues in food, environment, etc. is an important aspect of drug analysis. Food with overproof antibiotic residues after being eaten for a long time and people can be greatly harmed to human health after the food is eaten for a long time and the food is eaten for a long time in an environment with overproof antibiotic residues, so that the detection of ciprofloxacin residues increasingly draws attention of people.
Ciprofloxacin has the characteristics of wide antibacterial spectrum, strong antibacterial activity, low toxic and side effects, high clinical curative effect and the like, so that ciprofloxacin becomes the most widely clinically applied quinolone drugs at home and abroad at present, a standard detection method specified in Chinese pharmacopoeia is high performance liquid chromatography, and in addition, the reported method comprises the following steps: spectrophotometry, fluorometry, and the like. Compared with high performance liquid chromatography, the method has lower cost, is easier to implement, does not need enrichment and separation operation, is widely used for water quality detection and medical application, and is easier to implement.
Disclosure of Invention
The invention provides a fluorescent carbon dot and a fluorescence kit for detecting ciprofloxacin prepared by the same.
The technical scheme for realizing the invention is as follows:
a fluorescence kit for detecting ciprofloxacin comprises a solution A and a solution B, wherein the solution A is a prepared dull carbon dot, the solution A has no fluorescence, the solution A reacts for 15 minutes after the solution B is added, and a fluorescence spectrum is tested. Then ciprofloxacin is added into the two mixed solutions of the kit, and due to the fact that ciprofloxacin has a strong complexing effect with the divalent copper ions in the solution B, after reaction, the fluorescence of the mixed solution is weakened, and the test method is a fluorescence off-on-off type.
The preparation steps of the solution A are as follows: dissolving naphthalene derivative and dopamine analog in anhydrous ethanol, respectively performing ultrasonic treatment on the two solutions to completely dissolve the naphthalene derivative and dopamine analog, transferring the mixed solution into a microwave synthesis tube, and reacting in a microwave synthesizer at 60-190 ℃ for 1-100 min. And (3) after the reaction is finished, removing the mixture, centrifuging the mixture for 10 minutes at 10000 rpm, removing the precipitate, performing ultrafiltration, and storing the mixture as solution A, or performing spin-drying on the mixture to store the powder, and dissolving the powder according to the use concentration when in use.
Preferably, the naphthalene derivatives include aminonaphthalene compounds and hydroxynaphthalene compounds, and the dopamine analogs include dopamine or dopaquinone.
The amino naphthalene compound is 1, 3-dihydroxy naphthalene and an isomer thereof, and the hydroxy naphthalene compound is 1, 3-dihydroxy naphthalene and an isomer thereof; dopamine is dopamine hydrochloride, and dopaquinone is dopaquinone hydrochloride.
The molar ratio of the naphthalene derivative to the dopamine analogue is (100: 1) - (1: 100), the microwave reaction temperature is 60-190 ℃, and the reaction time is 1-100 min.
The solution B is a copper sulfate solution: weighing CuSO4•5H20.0125 g of O, dissolved in 5 mL of ultrapure water, and ultrasonically treated to completely dissolve the O to obtain solution B.
Preferably, the invention also provides a fluorescent carbon dot, and the specific preparation steps are as follows.
Specifically, the method for preparing the fluorescent carbon dots by using the 1, 3-dihydroxynaphthalene and the dopamine hydrochloride comprises the following steps:
0.0100 g (0.062 mmol) of 1, 3-dihydroxynaphthalene and 0.0118 g (0.062 mmol) of dopamine hydrochloride are weighed out and dissolved in 5 mL of absolute ethanol. And (3) respectively carrying out ultrasonic treatment on the two solutions to completely dissolve the two solutions, transferring the mixed solution into a microwave synthesis tube, and reacting for 30 minutes at 160 ℃ in a microwave synthesizer. And (3) after the reaction is finished, removing the mixture, centrifuging the mixture for 10 minutes at 10000 rpm, removing the precipitate, performing ultrafiltration, and storing the mixture as solution A, or performing spin-drying on the mixture to store the powder, and dissolving the powder according to the use concentration when in use.
The invention has the beneficial effects that:
(1) the method comprises the steps of taking the solution A and the solution B according to a certain proportion, adding a certain amount of ciprofloxacin into PBS buffer solution, and then carrying out fluorescence spectrum test. According to the established working curve, the content of ciprofloxacin can be calculated.
(2) The prepared carbon dot is used for preparing the kit, a fluorescence titration experiment is carried out, and the change of fluorescence intensity after ciprofloxacin with different concentrations is added is tested. After the ciprofloxacin is added, green fluorescence generated after the liquid A and the liquid B of the kit are mixed is weakened, the fluorescence intensity of the solution is reduced along with the increase of the concentration of the ciprofloxacin, and the rapid and quantitative detection of the ciprofloxacin can be realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a graph showing the change of fluorescence spectrum with time after mixing of the solutions A and B in example 1.
FIG. 2 shows fluorescence spectra of the system after 5 minutes, when ciprofloxacin (0-100. mu.M) was added at various concentrations after 15 minutes from the addition of copper ions (5. mu.M).
FIG. 3 is a photograph of the system after addition of copper ions at different concentrations under UV irradiation (365 nm).
FIG. 4 photographs of the system after 5 minutes under UV lamp irradiation (365 nm) with different concentrations of ciprofloxacin (0-100. mu.M) added 15 minutes after the addition of copper ions (5. mu.M).
FIG. 5 is a UV-Vis spectrum of the system after adding copper ions (5. mu.M) for 15 minutes and then adding ciprofloxacin (0-100. mu.M) at different concentrations for 5 minutes.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
The preparation method of the fluorescent carbon dot comprises the following steps:
0.0100 g (0.062 mmol) of 1, 3-dihydroxynaphthalene and 0.0118 g (0.062 mmol) of dopamine hydrochloride are weighed out and dissolved in 5 mL of absolute ethanol. And (3) respectively carrying out ultrasonic treatment on the two solutions to completely dissolve the two solutions, transferring the mixed solution into a microwave synthesis tube, and reacting for 30 minutes at 160 ℃ in a microwave synthesizer. And (3) after the reaction is finished, removing the mixture, centrifuging the mixture for 10 minutes at 10000 rpm, removing the precipitate, performing ultrafiltration, and storing the mixture as solution A, or performing spin-drying on the mixture to store the powder, and dissolving the powder according to the use concentration when in use.
Example 2
A kit for detecting ciprofloxacin comprising:
(1) solution A prepared in example 1 at a concentration of 40. mu.g/ml;
(2) the solution B is a copper sulfate solution: weighing CuSO4•5H20.0125 g of O, dissolved in 5 mL of ultrapure water, and dissolved completely by sonication.
(3) The solution A has no fluorescence, and after the solution B is added, the reaction is carried out for 15 minutes, and the fluorescence spectrum is tested.
(4) Ciprofloxacin is added into two mixed solutions of the kit, and due to the fact that ciprofloxacin and divalent copper ions in the solution B have strong complexation, after reaction, the fluorescence of the mixed solution is weakened, and the detection method is a fluorescence off-on-off type.
(5) And calculating the ciprofloxacin content in the sample according to the established working curve.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A fluorescence kit for detecting ciprofloxacin, which is characterized in that: the preparation method comprises a solution A and a solution B, wherein the solution A is a fluorescent carbon dot solution obtained by reacting a naphthalene derivative with a dopamine analogue, and the solution B is a copper solution.
2. The fluorescence kit according to claim 1, wherein the solution A is prepared by the steps of: dissolving the naphthalene derivative and the dopamine analogue in a solvent, and carrying out microwave reaction.
3. The fluorescence kit of claim 2, wherein: the naphthalene derivative comprises an aminonaphthalene compound and a hydroxynaphthalene compound, and the dopamine analogue comprises dopamine or dopaquinone.
4. The fluorescence kit of claim 3, wherein: the amino naphthalene compound is 1, 3-dihydroxy naphthalene and an isomer thereof, and the hydroxy naphthalene compound is 1, 3-dihydroxy naphthalene and an isomer thereof; dopamine is dopamine hydrochloride, and dopaquinone is dopaquinone hydrochloride.
5. The fluorescence kit of claim 1, wherein: the molar ratio of the naphthalene derivative to the dopamine analogue is (100: 1) - (1: 100), the microwave reaction temperature is 60-190 ℃, and the reaction time is 1-100 min.
6. Use of the fluorescence kit according to claim 5 for detecting ciprofloxacin, characterized in that: ciprofloxacin was added to the mixed solution of solution a and solution B, and a fluorescence spectrum test was performed.
7. A fluorescent carbon dot is applied to the fluorescent kit of claim 5, and is prepared by the following specific steps: dissolving the naphthalene derivative and the dopamine analogue in a solvent, and carrying out microwave reaction to obtain the product.
8. A fluorescent carbon dot according to claim 7, wherein: the naphthalene derivative is 1, 3-dihydroxynaphthalene, the dopamine analogue is dopamine hydrochloride, the molar ratio of the naphthalene derivative to the dopamine analogue is (100: 1) - (1: 100), the microwave reaction temperature is 60-190 ℃, the reaction time is 1-100min, and the reaction is followed by centrifugation.
9. The fluorescent carbon dot of claim 7 or 8 is mixed with a copper solution and then used as a reagent in a kit for detecting ciprofloxacin.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110641993.0A CN113433102B (en) | 2021-06-09 | 2021-06-09 | Fluorescent carbon dot and fluorescence kit for detecting ciprofloxacin prepared by same |
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| CN202110641993.0A CN113433102B (en) | 2021-06-09 | 2021-06-09 | Fluorescent carbon dot and fluorescence kit for detecting ciprofloxacin prepared by same |
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Citations (10)
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| CN101915844A (en) * | 2010-08-03 | 2010-12-15 | 中国农业大学 | Method and special quantum dot fluorescent immunoassay kit for detecting quinolone compounds |
| CN104597018A (en) * | 2015-01-22 | 2015-05-06 | 广西师范学院 | Method for detecting potassium permanganate by virtue of fluorescent carbon point probes |
| CN105675559A (en) * | 2016-01-14 | 2016-06-15 | 中国科学院理化技术研究所 | Method for detecting dopamine by using carbon dots as fluorescence probe |
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2021
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