CN113430234A - Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms - Google Patents
Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms Download PDFInfo
- Publication number
- CN113430234A CN113430234A CN202110718920.7A CN202110718920A CN113430234A CN 113430234 A CN113430234 A CN 113430234A CN 202110718920 A CN202110718920 A CN 202110718920A CN 113430234 A CN113430234 A CN 113430234A
- Authority
- CN
- China
- Prior art keywords
- anaerobic
- potential
- acid
- microorganisms
- chain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for producing medium-chain fatty acid by strengthening anaerobic microorganisms with external potential, which comprises the following steps: anaerobic acidogenic granular sludge is used as an inoculation bacteria source, potential is applied to domesticate and enrich microorganisms with chain extension function, and under the action of the microorganisms with the chain extension function completely domesticated, potential is applied, and acetic acid and ethanol are used as substrates to produce butyric acid and caproic acid through anaerobic fermentation. The invention adopts the method of external potential to produce medium-chain fatty acid, which not only shortens the reaction period, but also improves the yield of butyric acid.
Description
Technical Field
The invention belongs to the field of environmental microorganisms, and particularly relates to a method for producing medium-chain fatty acids by using an external potential reinforced anaerobic microorganism.
Background
The extension of the carbon chain of the microorganism is a novel biotechnology with low energy consumption and low operation cost, the medium-chain fatty acid generated in the process is a resource with higher economic value, and the organic pollutants can be removed while the resource is recycled. The microorganism carbon chain extension is a process of synthesizing medium-chain fatty acid by short-chain fatty acid by adding two carbon atoms in each cycle under the action of functional microorganisms.
At present, the method for producing butyric acid which is actually applied has the defects of strict technical conditions, high cost, secondary pollutant generation and the like, and is often accompanied with certain product loss. The microbial anaerobic fermentation technology has the advantages of mild reaction conditions, low treatment cost, environmental friendliness and the like. Compared with other upgrading technologies, the microbiological method has better economic benefits, but is limited by the participation of various functional microorganisms (mainly Clostridium clarkii), and the carbon chain elongation reaction is easily influenced by environmental factors and the activity of the functional microorganisms (such as pH, substrate concentration and the like).
Patent CN112391292A reports an enrichment method and application of microorganisms with medium-chain fatty acid carbon chain extension function, and 3.4g/L of butyric acid and 2.5g/L of caproic acid are finally obtained; patent CN111909970A reports that medium-chain fatty acids are produced by adding exogenous media and strengthening synthesis gas through anaerobic microorganisms, the maximum production of caproic acid can be 4.97g/L by adding iron-loaded platinum solid media in a reaction system, and the maximum production of butyric acid can be 3.43g/L by adding pyrolusite in the reaction system; patents CN110643644A and CN110643644B report methods for producing medium-chain fatty acids from fermentation broth of livestock and poultry manure; patent CN110656133A reports the production of medium-chain fatty acids by anaerobic fermentation of anaerobic sludge pretreated with ammonium chloride solution; patent CN110734934A reports the production of medium-chain fatty acids by pretreating anaerobic sludge with sodium nitrite solution; patent CN110734933A reports the production of medium-chain fatty acids by anaerobic fermentation with addition of zero-valent iron powder.
The methods for producing medium-chain fatty acids using anaerobic microorganisms described in the above-mentioned published patents are mostly methods in which an exogenous medium is added, and although production of butyric acid is promoted, the yield of butyric acid is low and the reaction period is long.
Disclosure of Invention
In order to overcome the defects of low utilization rate of a traditional anaerobic microorganism chain extension substrate, low yield of butyric acid and caproic acid and the like, the invention aims to provide a method for producing medium-chain fatty acid by using an external potential reinforced anaerobic microorganism.
The invention realizes domestication and application of chain extension functional microorganisms through external potential. Providing an electron donor and an electron acceptor (such as ethanol and acetic acid), and realizing the extension of the carbon chain under the action of the chain extension functional microorganism. The invention aims to improve the concentration of butyric acid and caproic acid and endow higher commercial value and economic value.
As shown in figure 1, the device for carbon chain extension provided by the invention successfully realizes domestication and enrichment of chain extension functional microorganisms from anaerobic sludge by taking anaerobic granular sludge as an environmental source of the chain extension functional microorganisms and applying a working potential to a system, and achieves the aim of quickly and efficiently producing butyric acid and caproic acid. Therefore, the method is beneficial to getting rid of the dilemma of low yield of the medium-chain fatty acid in China, and has important economic value and research significance.
The invention provides a method for producing medium-chain fatty acid by using an external potential to strengthen anaerobic microorganisms, which comprises the following steps: anaerobic acidogenic granular sludge is used as an inoculation bacteria source, potential is applied to domesticate and enrich microorganisms with chain extension function, and under the action of the microorganisms with the chain extension function completely domesticated, potential is applied, and acetic acid and ethanol are used as substrates to produce butyric acid and caproic acid through anaerobic fermentation.
The method comprises the following steps:
1) domestication and enrichment of chain-extending functional microorganisms
(1) Collecting anaerobic acid-producing granular sludge, performing heat treatment, and inhibiting the activity of methanogens to obtain pretreated anaerobic granular sludge;
(2) aerating the anaerobic granular sludge prepared in the step (1) to keep an anaerobic environment;
(3) inoculating the anaerobic granular sludge prepared in the step (2) into a microbial reactor, applying potential, starting the reactor, performing acclimation and enrichment of chain-extension-function microorganisms under the condition of high organic load, and completing acclimation of the chain-extension-function microorganisms when the concentration of ethanol in fermentation liquid is reduced and the concentration of volatile fatty acid is increased to be stable;
2) under the action of domesticated complete chain-extending functional microorganisms, applying potential, and producing butyric acid and caproic acid by anaerobic fermentation with acetic acid and ethanol as substrates.
In the step 1) (1) of the method, the anaerobic acid-producing granular sludge is obtained from an anaerobic digestion device of excess sludge of a sewage treatment plant of Minican in Beijing, the average grain diameter of the anaerobic digestion device is 0.6-2.5mm, and the ratio (VSS/TSS) of volatile suspended matters and total suspended matters of the anaerobic sludge is 0.4-0.8, and particularly 0.7;
the temperature of the sludge heat treatment in the step (1) can be 90-100 ℃, and the treatment time can be 1-1.5 h;
anaerobic granular sludge aeration for 20 minutes in step (2) (N)2:CO2＝80:20)；
The potential in step (3) can be-0.4V (-1) V (relative to a standard hydrogen electrode), and specifically can be-0.7V;
in the step (3), the high organic load is realized by the following operations:
after the reactor is started, the reactor is fed with water in a continuous flow mode;
the inlet water contains acetic acid, ethanol and 2-BES (2-bromoethyl sodium sulfonate: methanogen inhibitor), wherein the concentrations of the acetic acid, the ethanol and the 2-BES can be 6.8-30g/L, 4.8-25ml/L and 1-10g/L in sequence;
maintaining pH at 5-6.8, specifically 5.5-6.5 during acclimation period; the temperature can be 28-37 ℃, and the culture time can be 30-40 d.
In step 2), the potential may be-0.4V (-1) V (relative to a standard hydrogen electrode), and specifically may be-0.8V.
The application of the external potential in the domestication and enrichment of the chain-extending functional microorganisms also belongs to the protection scope of the invention.
In such applications, the potential may be-0.4V (-1) V (relative to a standard hydrogen electrode), and may specifically be-0.7V.
The application of the external potential to strengthen the production of the medium-chain fatty acid by the anaerobic microorganisms also belongs to the protection scope of the invention.
The application mechanism and the effect of the invention are as follows:
(1) the stability of an anaerobic system and the enrichment capacity of microorganisms are enhanced by adding the carbon brush electrode (12);
(2) the hydrogen ions transferred to the cathode region play a positive role in the reduction of acetic acid;
(3) the lower pH value can inhibit the growth of methanogens and promote the accumulation of butyrate;
(4) the mole ratio of acetic acid and ethanol is lower, and the larger organic load improves the production rate of butyric acid;
(5)2-BES inhibits the growth of methanogens and promotes chain extension reactions;
(6) the invention enriches chain-extension functional microorganisms from anaerobic granular sludge, and solves the problem of limited source of chain-extension microorganisms.
The invention adopts the method of external potential to produce medium-chain fatty acid, which not only shortens the reaction period, but also improves the yield of butyric acid.
Drawings
FIG. 1 is a schematic view of a reaction apparatus used in the present invention. Wherein, 1: a water inlet peristaltic pump; 2: a water inlet; 3: a sampling port; 4: a water outlet; 5: a gas collection port; 6: an electrochemical workstation; 7: a computer; 8: a gas collection bag; 9: a reference electrode; 10: anode (graphite rod electrode); 11: a three-phase separator; 12: a cathode (carbon brush electrode); 13: a heat tracing band; 14: a temperature control probe; 15: a water inlet barrel; 16: and (6) discharging the water bucket.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The invention provides a method for producing medium-chain fatty acid by using an external potential to strengthen anaerobic microorganisms, which comprises the following steps:
1) domestication and enrichment of chain-extending functional microorganisms
(1) Collecting anaerobic acid-producing granular sludge, performing heat treatment, and inhibiting the activity of methanogens to obtain pretreated anaerobic granular sludge;
(2) aerating the anaerobic granular sludge prepared in the step (1) to keep an anaerobic environment;
(3) inoculating the anaerobic granular sludge prepared in the step (2) into a microbial reactor, applying potential, starting the reactor, performing acclimation and enrichment of chain-extension-function microorganisms under the condition of high organic load, and completing acclimation of the chain-extension-function microorganisms when the concentration of ethanol in fermentation liquid is reduced and the concentration of volatile fatty acid is increased to be stable;
2) under the action of domesticated complete chain-extending functional microorganisms, applying potential, and producing butyric acid and caproic acid by anaerobic fermentation with acetic acid and ethanol as substrates.
In the step 1) (1) of the method, the anaerobic acid-producing granular sludge is obtained from an anaerobic digestion device of excess sludge of a small Hongmen sewage treatment plant in Beijing, the average grain diameter of the anaerobic digestion device is 0.6-2.5mm, and the ratio (VSS/TSS) of volatile suspended matters and total suspended matters of the anaerobic sludge is 0.70;
the temperature of the sludge heat treatment in the step (1) can be 90-100 ℃, and the treatment time can be 1-1.5 h;
anaerobic granular sludge aeration for 20 minutes in step (2) (N)2:CO2＝80:20)；
The potential in step (3) can be-0.4V (-1) V (relative to a standard hydrogen electrode), and specifically can be-0.7V;
in the step (3), the high organic load is realized by the following operations:
after the reactor is started, the reactor is fed with water in a continuous flow mode;
the inlet water contains acetic acid, ethanol and 2-BES (2-bromoethanesulfonic acid sodium salt: methanogen inhibitor), wherein the concentrations of acetic acid, ethanol and 2-BES can be 7.5g/L, 20ml/L and 5g/L in sequence;
maintaining pH at 5-6.8 during acclimation; the temperature can be 28-37 ℃, and the culture time can be 30-40 d.
In step 2), the potential may be-0.4V (-1) V (relative to a standard hydrogen electrode), and specifically may be-0.8V.
The application of the external potential in the domestication and enrichment of the chain-extending functional microorganisms also belongs to the protection scope of the invention.
In such applications, the potential may be-0.4V (-1) V (relative to a standard hydrogen electrode), and may specifically be-0.7V.
The application of the applied potential to strengthen the production of the medium-chain fatty acid by the anaerobic microorganisms also belongs to the protection scope of the invention.
The invention adopts the method of external potential to produce medium-chain fatty acid, which not only shortens the reaction period, but also improves the yield of butyric acid.
Example 1
The chain extension functional microorganism is domesticated and enriched by the following method:
(1) collecting a fresh residual sludge anaerobic digestion device from a small Hongmen sewage treatment plant in Beijing, wherein the average particle size of the residual sludge anaerobic digestion device is 0.6-2.5mm, the ratio (VSS/TSS) of volatile suspended matters and total suspended matters of anaerobic sludge is 0.70, uniformly shaking the residual sludge anaerobic digestion device, placing the residual sludge anaerobic digestion device in a beaker for heating treatment in a water bath at 100 ℃, wherein the treatment time is 1h, and inhibiting the activity of methanogens to obtain pretreated anaerobic granular sludge;
(2) n is used for anaerobic granular sludge prepared in the step (1)2：CO2Aerating mixed gas at the ratio of 80:20 for 20min, and keeping an anaerobic environment;
(3) inoculating the treated activated sludge into a 1L microbial reactor (shown in figure 1); opening the electrochemical workstation 6, applying a specific potential of-0.7V (relative to a standard hydrogen electrode) to the working electrode 12 (cathode: carbon brush electrode) of the anaerobic reactor, feeding water in a continuous flow by means of a laboratory peristaltic pump (Badin Lange BT 100-2J); the inlet water contains acetic acid, ethanol and 2-BES (2-bromoethyl sodium sulfonate: methanogen inhibitor), and the concentrations of the acetic acid, the ethanol and the 2-BES are respectively 7.5g/L, 20ml/L and 5 g/L; during acclimation the pH was maintained at 5.8; the temperature is 35 ℃; domestication and enrichment of chain extension functional microorganisms are carried out, and the culture time is 40 days.
(4) And (3) completing the domestication of the chain-extending functional microorganisms when the concentration of the ethanol in the fermentation solution is reduced and the concentration of the volatile fatty acid is increased to be stable.
Example 2
The device for producing medium-chain fatty acid by microorganism carbon chain extension is shown in figure 1.
Inoculating the fully domesticated functional microorganisms of example 1 into 2 anaerobic reactors as shown in figure 1;
the continuous flow mode water inlet comprises water inlet containing acetic acid, ethanol and 2-BES (2-bromoethanesulfonic acid sodium salt: methanogen inhibitor), wherein the concentrations of the acetic acid, the ethanol and the 2-BES are respectively 6.8g/L, 22ml/L and 2.5 g/L; the temperature is 35 ℃;
opening the electrochemical workstation 6, applying a specific potential of-0.8V (relative to a standard hydrogen electrode) to the working electrode 12 (cathode: carbon brush electrode) of one of the anaerobic reactors; the other is not applied with electric potential;
by adopting the method, the invention can achieve the following effects:
the reaction is applied with a cathode potential of-0.8V, butyric acid is rapidly produced in the system, the yield of butyric acid reaches 137.5mM in the fifth day, and the yield of caproic acid is 3.3 mM; in the next 15 days, the yield of butyric acid is reduced and is maintained at about 100mM, and the yield of caproic acid is increased to 24.5 mM; the acetic acid conversion rate reaches 80-90%; the conversion rate of the ethanol reaches 85 to 90 percent; the yield of butyric acid is reduced from 60% to about 45% along with the generation of caproic acid; the total carbon efficiency is basically kept at about 70-80%; in the reactor without the working potential, butyric acid reached a maximum concentration of 125.1mM around the twentieth day and caproic acid reached 15.2mM at day 60; the acetic acid conversion rate is basically maintained at 80-85%, and the ethanol conversion rate is about 70-80%. Compared with the potential without adding, the butyric acid and the caproic acid generated by the potential with-0.8V are respectively increased by 12.4mM and 9.3mM, the acetic acid conversion rate is increased by about 5 percent, and the ethanol conversion rate is increased by 10 to 15 percent.
The reaction system can generate 137.5mM butyric acid and 24.5mM caproic acid in a short time by applying-0.8V potential, high utilization and conversion of acetic acid and ethanol are realized, meanwhile, the carbon brush electrode promotes the enrichment of functional microorganisms, and electrons in the cathode region play a positive reduction role on the acetic acid, thereby promoting the generation of the butyric acid and the caproic acid.
The reaction system has certain influence on the production of butyric acid and caproic acid by applying different working potentials. The reaction is applied with a cathode potential of-0.7V, the yield of butyric acid is basically maintained at about 97mM, and the highest yield of caproic acid is 19.5 mM; the reaction is applied with a cathode potential of-0.6V, the yield of butyric acid is basically maintained at about 116mM, and meanwhile, the yield of caproic acid is reduced to about 13 mM; the reaction is applied with a cathode potential of-0.5V, the yield of butyric acid is maintained at about 100mM, and the caproic acid is increased to 19 mM; the yield of butyric acid and caproic acid of the reaction at-0.4V applied with a cathodic potential was maintained at about 120mM and at about 5 mM.
The production of butyric acid and caproic acid can be realized in a short time by applying the working potential, but the production of butyric acid and caproic acid is different by different potentials.
Claims (10)
1. A method for strengthening the production of medium-chain fatty acid by anaerobic microorganisms by applying an external potential comprises the following steps: anaerobic acidogenic granular sludge is used as an inoculation bacteria source, potential is applied to domesticate and enrich microorganisms with chain extension function, and under the action of the microorganisms with the chain extension function completely domesticated, potential is applied, and acetic acid and ethanol are used as substrates to produce butyric acid and caproic acid through anaerobic fermentation.
2. The method of claim 1, wherein: the method comprises the following steps:
1) domestication and enrichment of chain-extending functional microorganisms
(1) Collecting anaerobic acid-producing granular sludge, performing heat treatment, and inhibiting the activity of methanogens to obtain pretreated anaerobic granular sludge;
(2) aerating the anaerobic granular sludge prepared in the step (1) to keep an anaerobic environment;
(3) inoculating the anaerobic granular sludge prepared in the step (2) into a microbial reactor, applying potential, starting the reactor, performing acclimation and enrichment of chain-extension-function microorganisms under the condition of high organic load, and completing acclimation of the chain-extension-function microorganisms when the concentration of ethanol in fermentation liquid is reduced and the concentration of volatile fatty acid is increased to be stable;
2) under the action of domesticated complete chain-extending functional microorganisms, applying potential, and producing butyric acid and caproic acid by anaerobic fermentation with acetic acid and ethanol as substrates.
3. The method of claim 2, wherein: in the step 1) (1), the anaerobic acid-producing granular sludge is taken from an UASB reactor for treating citric acid wastewater, the average grain diameter of the anaerobic acid-producing granular sludge is 0.6-2.5mm, and the ratio of volatile suspended matters to total suspended matters of the anaerobic sludge is 0.70.
4. A method according to claim 2 or 3, characterized in that: the temperature of the sludge heat treatment in the step (1) is 90-100 ℃, and the treatment time is 1-1.5 h;
aerating anaerobic granular sludge for 20 minutes in step (2), and N2:CO2＝80:20。
5. The method according to any one of claims 2-4, wherein: the potential in step (3) is-0.4V (-1) V relative to a standard hydrogen electrode.
6. The method according to any one of claims 2-5, wherein: the high organic load is achieved by:
after the reactor is started, the reactor is fed with water in a continuous flow mode;
the inlet water contains acetic acid, ethanol and 2-BES, wherein the concentrations of the acetic acid, the ethanol and the 2-BES are 7.5g/L, 20ml/L and 5g/L in sequence;
maintaining pH at 5-6.8 during acclimation; the temperature is 28-37 ℃, and the culture time is 30-40 d.
7. The method according to any one of claims 2-6, wherein: in the step 2), the potential is-0.4V (-1) V relative to a standard hydrogen electrode.
8. The application of external potential in domestication and enrichment of chain-extending functional microorganisms.
9. Use according to claim 8, characterized in that: in the application, the potential is-0.4V (-1) V relative to a standard hydrogen electrode.
10. The application of the applied potential to strengthen the production of medium-chain fatty acid by anaerobic microorganisms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110718920.7A CN113430234B (en) | 2021-06-28 | 2021-06-28 | Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110718920.7A CN113430234B (en) | 2021-06-28 | 2021-06-28 | Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113430234A true CN113430234A (en) | 2021-09-24 |
| CN113430234B CN113430234B (en) | 2022-11-22 |
Family
ID=77754919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110718920.7A Active CN113430234B (en) | 2021-06-28 | 2021-06-28 | Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113430234B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114292884A (en) * | 2021-12-23 | 2022-04-08 | 江苏大学 | Method for synthesizing caproic acid by high-yield sludge open system |
| CN115418377A (en) * | 2022-11-03 | 2022-12-02 | 哈尔滨工业大学 | Method for producing caproic acid by reinforcing anaerobic microorganisms through external magnetic field |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2250273A2 (en) * | 2008-02-28 | 2010-11-17 | Green Biologics Limited | Production process |
| CN104651440A (en) * | 2015-01-14 | 2015-05-27 | 同济大学 | Biological extension method for electrically promoted carbon chain and biological extension device for electrically promoted carbon chain |
| CN110734934A (en) * | 2019-10-30 | 2020-01-31 | 同济大学 | method for producing medium-chain fatty acid by promoting anaerobic fermentation of excess sludge through pretreatment |
| CN111909970A (en) * | 2020-08-10 | 2020-11-10 | 北京化工大学 | Method for producing medium-chain fatty acid by fermentation of exogenous medium reinforced anaerobic microorganisms |
| CN112391292A (en) * | 2020-11-23 | 2021-02-23 | 江南大学 | Medium-chain fatty acid carbon chain extension functional microorganism and enrichment method and application thereof |
| CN112481316A (en) * | 2020-06-23 | 2021-03-12 | 哈尔滨工业大学 | Cathode electric fermentation method for preparing butyric acid by fermenting straw through enhanced anaerobic mixed flora |
| CN112795600A (en) * | 2021-03-05 | 2021-05-14 | 山东兆盛天玺环保科技有限公司 | Method for producing caproic acid by adopting electric fermentation to strengthen short-chain volatile fatty acid addition |
-
2021
- 2021-06-28 CN CN202110718920.7A patent/CN113430234B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2250273A2 (en) * | 2008-02-28 | 2010-11-17 | Green Biologics Limited | Production process |
| CN104651440A (en) * | 2015-01-14 | 2015-05-27 | 同济大学 | Biological extension method for electrically promoted carbon chain and biological extension device for electrically promoted carbon chain |
| CN110734934A (en) * | 2019-10-30 | 2020-01-31 | 同济大学 | method for producing medium-chain fatty acid by promoting anaerobic fermentation of excess sludge through pretreatment |
| CN112481316A (en) * | 2020-06-23 | 2021-03-12 | 哈尔滨工业大学 | Cathode electric fermentation method for preparing butyric acid by fermenting straw through enhanced anaerobic mixed flora |
| CN111909970A (en) * | 2020-08-10 | 2020-11-10 | 北京化工大学 | Method for producing medium-chain fatty acid by fermentation of exogenous medium reinforced anaerobic microorganisms |
| CN112391292A (en) * | 2020-11-23 | 2021-02-23 | 江南大学 | Medium-chain fatty acid carbon chain extension functional microorganism and enrichment method and application thereof |
| CN112795600A (en) * | 2021-03-05 | 2021-05-14 | 山东兆盛天玺环保科技有限公司 | Method for producing caproic acid by adopting electric fermentation to strengthen short-chain volatile fatty acid addition |
Non-Patent Citations (7)
| Title |
|---|
| JIANG Y, ET AL.: "Microbial electrochemical stimulation of caproate production from ethanol and carbon dioxide", 《BIORESOUR TECHNOL》 * |
| VAN EERTEN-JANSEN M C AA,ET AL.: "Bioelectrochemical Production of Caproate and Caprylate from Acetate by Mixed Cultures", 《ACS SUSTAINABLE CHEMISTRY & ENGINEERING》 * |
| YONG J, ET AL.: "Electro-fermentation regulates mixed culture chain elongation with fresh and acclimated cathode", 《ENERGY CONVERSION AND MANAGEMENT》 * |
| 中国能源学会: "《第三届能源科学家论坛 论文集 下》", 29 February 2012, 中国环境科学出版社 * |
| 刘昊鹏等: "基于厌氧微生物的碳链延长合成高价值化学品反应机理及研究进展:不同电子供体", 《北京化工大学学报（自然科学版）》 * |
| 徐功娣: "《微生物燃料电池原理与应用》", 30 November 2012, 哈尔滨工业大学出版社 * |
| 成双兰: "生物电催化强化己酸合成及其碳链延伸机理研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技I辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114292884A (en) * | 2021-12-23 | 2022-04-08 | 江苏大学 | Method for synthesizing caproic acid by high-yield sludge open system |
| CN115418377A (en) * | 2022-11-03 | 2022-12-02 | 哈尔滨工业大学 | Method for producing caproic acid by reinforcing anaerobic microorganisms through external magnetic field |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113430234B (en) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Salerno et al. | Inhibition of biohydrogen production by ammonia | |
| Mohan et al. | Biohydrogen production from chemical wastewater treatment in biofilm configured reactor operated in periodic discontinuous batch mode by selectively enriched anaerobic mixed consortia | |
| Qu et al. | Bio-hydrogen production by dark anaerobic fermentation of organic wastewater | |
| Xu et al. | On-site CO2 bio-sequestration in anaerobic digestion: Current status and prospects | |
| Aryal et al. | Microbial electrochemical approaches of carbon dioxide utilization for biogas upgrading | |
| CN108314184B (en) | Method for promoting start of anaerobic reactor | |
| CN103555566B (en) | Novel external electrolysis device for promoting anaerobic digestion to produce methane | |
| CN113430234B (en) | Method for producing medium-chain fatty acid by using external potential to strengthen anaerobic microorganisms | |
| CN104108845B (en) | A kind of microbiological fuel cell utilizes the methanogenic method of excess sludge synchronous electrogenesis | |
| CN104529116A (en) | Method for utilizing nano ferroferric oxide for improving activity of anaerobic digestion methanogens and methanogenesis efficiency | |
| NL2026669B1 (en) | A process to treat a carbon dioxide comprising gas | |
| CN112795600B (en) | Method for producing caproic acid by adopting electric fermentation to strengthen short-chain volatile fatty acid addition | |
| CN109161476A (en) | A kind of apparatus and method of electricity fermentation methane phase | |
| Zhao et al. | Engineering enhanced anaerobic digestion: Benefits of ethanol fermentation pretreatment for boosting direct interspecies electron transfer | |
| CN104651440A (en) | Biological extension method for electrically promoted carbon chain and biological extension device for electrically promoted carbon chain | |
| Zhang et al. | Metabolic pathways of hydrogen production in fermentative acidogenic microflora | |
| CN106480102A (en) | A kind of method for improving methane production using electrolysis auxiliary anaerobe | |
| CN104263764A (en) | Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge | |
| Yang et al. | Understanding and design of two-stage fermentation: A perspective of interspecies electron transfer | |
| CN109022491B (en) | Hydrogen alkane fermentation coupling recycling process for poultry and livestock manure | |
| CN116732111A (en) | Method for promoting conversion of waste biomass energy by adopting persulfate oxidation and sulfate reduction bacteria novel system | |
| CN111470744A (en) | Method for producing volatile organic acid by anaerobic fermentation of sludge | |
| NL2029926B1 (en) | A process to treat a carbon dioxide comprising gas | |
| NL2029927B1 (en) | A process to treat a carbon dioxide comprising gas | |
| KR102463900B1 (en) | System for producing biohydrogen comprising microbial electrolysis cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |