CN113425655B - Method for extracting coix seed active substance - Google Patents
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- CN113425655B CN113425655B CN202110678305.8A CN202110678305A CN113425655B CN 113425655 B CN113425655 B CN 113425655B CN 202110678305 A CN202110678305 A CN 202110678305A CN 113425655 B CN113425655 B CN 113425655B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention relates to an extraction method of coix seed active substances, which specifically comprises the following steps: s1: taking coix seed raw material, and preprocessing to obtain coix seed powder; s2: mixing the coix seed powder obtained in the step S1 with water and an auxiliary agent to obtain an extracting solution, and then extracting to obtain the coix seed active substance, wherein the auxiliary agent contains amino. Compared with the prior art, the invention improves the extraction efficiency of the coix seed active substances, and the added auxiliary agents are all conventional raw materials of cosmetics, the dosage is small, the content in the finished product is small, separation is not needed, and the extracted solid can be directly used for the production of cosmetics.
Description
Technical Field
The invention relates to the technical field of bioactive substance extraction, in particular to a method for extracting coix seed active substances.
Background
The coix seed is taken as a traditional medicinal and edible plant in China, has rich nutritional value and medicinal value, has the effects of promoting diuresis, removing dampness, strengthening spleen, relieving diarrhea, removing arthralgia, expelling pus, detoxicating and resolving hard mass, and can be used for treating diseases such as edema, beriberi, dysuria, spleen deficiency diarrhea, damp arthralgia, pulmonary abscess, intestinal abscess, wart, cancer and the like. In recent years, students at home and abroad research on chemical components of coix seeds by TLC, HPLC, GC and the like, and find that the coix seeds contain various active substances, mainly comprising coix seed esters, triglycerides, fatty acids, lactams, coix seed lactones, saccharides, sterols, polyphenols, polysaccharides, proteins and the like, and further comprise other components, such as flavonoids, triterpenes, alkaloids and the like. Is beneficial to modern scientific technology, and coix seed active substances are widely applied in the industries of foods, nourishments, cosmetics, medicines and the like.
At present, the extraction method of the active ingredients of coix seeds is usually a traditional water extraction method, and in order to improve the extraction efficiency, related researchers propose a plurality of auxiliary methods based on the water extraction method, such as an enzyme auxiliary method, an ultrasonic auxiliary water extraction method, a microwave auxiliary method and an ethanol water extraction method, but the effect of the auxiliary methods on improving the extraction rate is still not obvious.
Disclosure of Invention
The invention aims to provide the extraction method of the coix seed active substance, which improves the extraction efficiency of the coix seed active substance, and the added auxiliary agents are all conventional raw materials of cosmetics, so that the dosage is small, the content in the finished product is small, separation is not needed, and the extracted solid can be directly used for the production of cosmetics.
The aim of the invention is achieved by the following technical scheme:
a method for extracting coix seed active substances comprises the following steps:
s1: taking coix seed raw material, and preprocessing to obtain coix seed powder;
s2: mixing the coix seed powder obtained in the step S1 with water and an auxiliary agent to obtain an extracting solution, and then extracting to obtain the coix seed active substance, wherein the auxiliary agent contains amino.
In step S1, the pretreatment process specifically includes: pulverizing Coicis semen, sieving, and drying to constant weight.
Sieving with 40 mesh sieve.
In step S2, the auxiliary agent includes one or more of betaine, triethanolamine or urea.
In the step S2, the dosage of the auxiliary agent is 0.1-20% of the weight of the coix seed powder.
In the step S2, the liquid-to-material ratio of water to coix seed powder is 5:1 (mL/g).
In step S2, extraction is performed in a thermostatic water bath.
In step S2, the extraction temperature is 80deg.C, and the extraction time is 0-120min, but not 0.
In the step S2, after the extraction is completed, extracting solution is filtered, upper liquid phase is collected, and coix seed active substances are obtained after drying.
In the step S2, after the extraction is completed, centrifuging the extracting solution, collecting an upper liquid phase, and drying to obtain the coix seed active substance.
The invention is based on the traditional water extraction method (the method takes water as a solvent, heats or boils substances, dissolves active ingredients in the water, finally separates out the substances stable to humidity and heat), adds proper amino-containing auxiliary agents (which are common additives in the fields of cosmetics and foods), and then obtains coix seed active substances through centrifugation or filtration, thereby effectively improving the extraction efficiency of the active substances. In the extraction process, the amino group on the amino group-containing auxiliary agent plays the following roles: 1, hydrogen bonding. Can form hydrogen bond with hydroxyl groups on cell walls and cell membranes, and promote dissolution of polyphenol structures. 2. The amino group has positive electrical properties. Amino groups can bind to cellulose or the like having a negative charge, changing its electrical properties. Similar to the principle of eutectic extraction. According to the types of the auxiliary agents, the auxiliary agents with different dosages are added to obtain the maximum yield of the active substances of the coix seeds.
Drawings
FIG. 1 is a graph showing the relationship between the yield of the active material of coix seed and the types and amounts of auxiliary agents in comparative example 1, examples 5 to 7, examples 10 and 11, examples 14 to 16, and examples 18 to 21.
Detailed Description
The invention will now be described in detail with reference to the drawings and specific examples.
Comparative example 1
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating the yield of Coicis semen active material to 3.8% as shown in figure 1.
Comparative example 2
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And then carrying out suction filtration on the extracting solution, collecting an upper liquid phase, drying in a drying oven, weighing and calculating the yield of the coix seed active product to be 3.4%.
Example 3
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.01g of triethanolamine (accounting for 0.1% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain active substance of Coicis semen with yield of 4.3%.
The solid obtained by drying is a solid mixture of the auxiliary agent and the coix seed active substance, and the auxiliary agent is a common additive in cosmetics and the like, so that the use effect of the coix seed active substance is not affected, the auxiliary agent is not required to be separated from the solid mixture, the yield of the coix seed active substance is the weight of the solid mixture minus the weight of the auxiliary agent, and a large amount of cellulose contained in the coix seed raw material is separated in the filtration and centrifugation processes. The method for testing the content of the active substances of the coix seeds comprises the following steps: after the extraction is completed, the clear liquid without solid powder is separated. From each experimental group (namely, the embodiment cases 3-21, the comparison cases 1 and 2 are modified by referring to the calculation formula) with different addition amounts of different auxiliary agents and different proportions, 25mL of leaching solution (namely, the clear solution above) is collected, and the leaching solution is placed into a blast oven for drying at a constant temperature of 80 ℃ and then weighed until the weight is constant X. The yield of the coix seed active substance is Y, and the calculation formula is as follows: y= (60×x/25-Z)/10×100%, where 60 is the total mass of the total solid-liquid mixture when the coix seed powder is 10g and the solid-liquid ratio is 5:1, 25 is the mass of the removed 25mL extract, 10 is the mass of 10g coix seed powder, and Z is the gram number of the additive, if in actual application, the numerical values in the calculation formula can be modified according to the mass of the actually added coix seed powder, the mass of the removed water, the mass of the removed extract, and the like.
Example 4
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.1g of triethanolamine (accounting for 1% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, and heating and refluxing at 80 ℃ for 120min to finish extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain active substance of Coicis semen with yield of 4.8%.
Example 5
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.5g of triethanolamine (accounting for 5% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, and heating and refluxing at 80 ℃ for 120min to finish extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 5.1% as shown in figure 1.
Example 6
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 1g of triethanolamine (accounting for 10% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 6.8% as shown in figure 1.
Example 7
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 2g of triethanolamine (accounting for 20% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 7.6% as shown in figure 1.
Example 8
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.01g of betaine (accounting for 0.1% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain 3.9% of Coicis semen active substance.
Example 9
1. Pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.1g of betaine (accounting for 1% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, and heating and refluxing at 80 ℃ for 120min to finish extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain active substance of Coicis semen of 4.0%.
Example 10
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.5g of betaine (accounting for 5% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 4.0% as shown in figure 1.
Example 11
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 2g of betaine (accounting for 20% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material of 4.7% as shown in figure 1.
Example 12
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.01g of urea (accounting for 0.1% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain 3.8% of Coicis semen active substance.
Example 13
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.1g of urea (accounting for 1% of the weight of the coix seed powder) to obtain an extracting solution, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extract (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating to obtain 3.6% of Coicis semen active substance.
Example 14
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 0.5g of urea (accounting for 5% of the weight of the coix seed powder) to obtain an extracting solution, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating the yield of Coicis semen active material to 3.6% as shown in figure 1.
Example 15
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 1g of urea (accounting for 10% of the weight of the coix seed powder) to obtain an extracting solution, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 4.1% as shown in figure 1.
Example 16
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 2g of urea (accounting for 20% of the weight of the coix seed powder) to obtain an extracting solution, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material of 4.4% as shown in figure 1.
Example 17
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 2g of triethanolamine (accounting for 20% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And then filtering and separating the extracting solution, collecting a liquid phase, drying in an oven, weighing and calculating the yield of the coix seed active product to be 7.4 percent.
Example 18
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 1.5g of triethanolamine (15% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, and heating and refluxing at 80 ℃ for 120min to finish extraction.
3. And then the extracting solution is filtered and separated by suction, liquid phase is collected, dried in an oven, weighed and the yield of the coix seed active product is calculated to be 7.8 percent, as shown in figure 1.
Example 19
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 21g of betaine (accounting for 10% of the weight of the coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material of 4.3% as shown in figure 1.
Embodiment example 20
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 1.5g of betaine (accounting for 15% of the weight of coix seed powder) to obtain an extract, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 4.9% as shown in figure 1.
Example 21
A method for extracting active substances from Coicis semen comprises the following steps:
1. pulverizing Coicis semen with pulverizer, sieving with 40 mesh sieve, drying, and constant weight.
2. Accurately weighing 10g of coix seed powder, placing into a three-neck flask, adding 50mL of purified water, adding 1.5g of urea (accounting for 15% of the weight of the coix seed powder) to obtain an extracting solution, stirring to uniformly mix the raw materials, heating and refluxing at 80 ℃ for 120min, and finishing the extraction.
3. And centrifuging the extractive solution (10000 r/min,15 min), collecting supernatant and oil phase, oven drying, weighing, and calculating yield of Coicis semen active material to 4.8% as shown in figure 1.
Comparing cases 1 and 2 with cases 3-21, it can be found that mixing the adjuvant with water and Coicis semen powder can promote extraction of Coicis semen active substances, increase yield of Coicis semen active substances, and can obtain maximum yield of Coicis semen active substances by adding adjuvants with different amounts according to the types of adjuvants.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (1)
1. The extraction method of the coix seed active substance is characterized by comprising the following steps of:
s1: taking coix seed raw material, and preprocessing to obtain coix seed powder;
s2: mixing the coix seed powder obtained in the step S1 with water and an auxiliary agent to obtain an extracting solution, and then extracting to obtain a mixture of the auxiliary agent and coix seed active substances, wherein the auxiliary agent contains amino;
wherein the auxiliary agent is triethanolamine;
the dosage of the auxiliary agent is 15% of the weight of the coix seed powder;
in step S1, the pretreatment process specifically includes: firstly, crushing coix seed raw materials, sieving by adopting a 40-mesh sieve, and drying to constant weight;
in the step S2, the liquid-material ratio of water to coix seed powder is 5:1 (mL/g);
in the step S2, extracting in a constant-temperature water bath;
in the step S2, the extraction temperature is 80 ℃, and the extraction time is 120min;
in the step S2, after the extraction is completed, extracting solution is filtered, upper liquid phase is collected, and coix seed active substances are obtained after drying;
wherein the yield of the coix seed active substance is 7.8%.
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| CN113425655A (en) | 2021-09-24 |
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