CN113423421A - 制备和使用通用中心化流感疫苗基因的方法 - Google Patents

制备和使用通用中心化流感疫苗基因的方法 Download PDF

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CN113423421A
CN113423421A CN201980074988.7A CN201980074988A CN113423421A CN 113423421 A CN113423421 A CN 113423421A CN 201980074988 A CN201980074988 A CN 201980074988A CN 113423421 A CN113423421 A CN 113423421A
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E·A·韦弗
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Abstract

本公开文本描述了许多不同的多肽序列以及编码此类多肽序列的核酸序列，所述编码此类多肽序列的核酸序列可以单独使用或以通用疫苗的形式组合使用(例如，针对人的甲型流感或乙型流感或猪的流感)。

Description

制备和使用通用中心化流感疫苗基因的方法
技术领域
本公开文本总体上涉及疫苗并且具体地涉及流感疫苗。
背景技术
1931年流感病毒被鉴定为猪发热病的病原体。两年后在1933年在人类中发现了流感病毒。
每年，世界人口中有5％-15％受到流行病的影响并且患有上呼吸道感染，3百万至5百万患有严重疾病，并且250,000至500,000例导致死亡(WHO 2009)。在美国，每年季节性流感影响多达20％的人口，导致200,000例住院治疗和大约37,000例死亡。世界卫生组织(WHO)指出，“流感以季节性流行病迅速传播到世界各地，并且以医院和其他医疗保健费用以及生产力损失的形式造成了相当大的经济负担。在美利坚合众国，据估计每年流感流行花费高达1670亿美元。”
尽管这些季节性感染当然值得关注，但大流行性流感的暴发和致病性流感的故意释放实质上更值得关注。在过去的一个世纪中，发生了若干次严重的大流行(WHO 2009)。1918-1919年，称为西班牙流感的H1N1流感引起了世界上最大的流感大流行，造成2000-4000万人死亡。1957年，由H2N2病毒引起的亚洲流感导致约150万人死亡，并且1968年，由H3N2流感引起的中国香港流感导致约100万人死亡。
在20世纪90年代中期之前，猪的主要循环病毒毒株是经典猪甲型流感H1N1。自1997年以来，已出现了若干种新的猪流感病毒毒株，包括H1N1、H3N2和H1N2。新出现的三元重组株(triple reassortant)是禽流感基因、人流感基因和猪流感基因的组合。在2009年3月，猪流感出现在墨西哥以及美国的加利福尼亚州和德克萨斯州两者。这种新型猪流感在全球传播，并且感染了世界人口的24％。
由于猪对人流感、禽流感和猪流感易感，因此它们充当混合容器以创建新的重组流感病毒。不仅存在来自感染流感的猪的人畜共患病的风险，而且猪的甲型流感病毒(IAV-S)是猪最重要的病原体之一。所述病毒在世界范围内广泛传播，给养猪者造成了巨大的经济损失。临床上，感染IAV-S的猪通常显示出急性呼吸道疾病的体征，所述体征在7-10天后迅速消退。然而，当与猪呼吸道疾病综合征的其他病原体相关联时，猪的IAV-S感染通常导致严重的肺炎并且甚至导致死亡。一项研究估计，由猪流感造成的损失导致美国养猪业大约700,000,000美元的损失。
因此，非常需要一种有效针对相异流感病毒毒株的流感疫苗。本公开文本提供了制备和使用通用中心化流感疫苗基因的方法。
发明内容
本文描述的通用疫苗基因是使用本文描述的若干种独特策略创建的，并且经过计算优化以提供最大可能的疫苗效力。这些独特的序列可以允许生产用于预防流感病毒感染的通用疫苗，所述通用疫苗提供了针对所有相异流感病毒毒株的近乎完全的保护作用。
在一方面，提供了与选自SEQ ID NO:1-102中的任一个的氨基酸序列具有至少90％序列同一性(例如，至少95％序列同一性；至少99％序列同一性；或100％序列同一性)的疫苗多肽。
在一些实施方案中，所述疫苗多肽与选自SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95和100-102的氨基酸序列具有至少90％序列同一性(例如，至少95％序列同一性；至少99％序列同一性；或100％序列同一性)。
在一些实施方案中，所述疫苗多肽与选自SEQ ID NO:3、6、17、20、46、49、54和57的氨基酸序列具有至少90％序列同一性(例如，至少95％序列同一性；至少99％序列同一性；或100％序列同一性)。
在一些实施方案中，所述多肽是由与选自SEQ ID NO:103-122的核酸序列具有至少90％序列同一性(例如，至少95％序列同一性；至少99％序列同一性；或100％序列同一性)的核酸序列编码的。
在另一方面，提供了包含本文所述的疫苗多肽中的至少一种的疫苗组合物以及递送媒介物。在一些实施方案中，所述递送媒介物是病毒。代表性病毒包括但不限于腺病毒、腺相关病毒、复逆转录病毒、甲病毒、副粘病毒或弹状病毒。在一些实施方案中，所述递送媒介物是药学上可接受的载体。代表性的药学上可接受的载体是生理盐水。在一些实施方案中，所述递送媒介物是纳米颗粒(例如，脂质纳米颗粒)。在一些实施方案中，疫苗组合物包含至少两种疫苗多肽、至少三种疫苗多肽、至少四种疫苗多肽、至少八种疫苗多肽或至少十二种疫苗多肽。
在又另一方面，提供了一种向受试者接种疫苗的方法。这种方法通常包括向需要疫苗接种的受试者施用如本文所述的中心化疫苗多肽或如本文所述的疫苗组合物。代表性受试者包括人和猪。在一些实施方案中，所述施用步骤重复多于一次。
在一些实施方案中，如本文所述的疫苗多肽是针对至少约3种不同病毒亚型的通用疫苗。在一些实施方案中，如本文所述的疫苗多肽是针对至少约12种病毒亚型的通用疫苗。
定义
抗原和免疫原在整个本公开文本中可互换使用。两者都指诱导免疫应答的抗原蛋白。
通用疫苗是指能够提供针对流感亚型内的大多数变体以及1组和2组流感病毒内的亚型的保护作用的疫苗。通用疫苗可以使用多种免疫原提供保护作用。
中心化序列是指模拟过去几年中流感感染的祖先的序列。中心化序列(例如，多肽)被设计为定位至流感病毒的亚型的系统发育树的中心。本文所述的中心化序列是使用表示代表性系统发育树的主要分支的单独野生型序列创建的。这种方法的基本原理是产生相对于所有其他变体定位在中心的免疫原。因此，这种蛋白质与所有变体的序列差异都低于任何两个随机选择的基因。
除非另外定义，否则本文中使用的所有技术术语和科学术语具有与所述方法和物质组合物所属领域的普通技术人员通常所理解的含义相同的含义。尽管与本文所述的方法和材料类似或等同的那些方法和材料可以用于所述方法和物质组合物的实践或测试中，但下文描述了合适的方法和材料。此外，所述材料、方法和实施例仅是说明性的，而非限制性的。本文提及的所有出版物、专利申请、专利和其他参考文献都通过引用以其整体并入。
附图说明
图1是显示中心化血凝素基因的系统发育分析的示意图。共有基因与野生型基因之间的遗传关系显示在无根邻接系统发育树中。显示H1、H2、H3和H5共有序列COT基因定位至树的中心。黑色条指示HA氨基酸序列的差异百分比。中心树(虚线)示出了HA亚型之间的遗传多样性的程度。
图2是显示通过野生型和中心化HA免疫原得到的针对流感病毒感染的保护作用的图。用表达A/PR/8/34、H1-COT和A/CA/04/09HA的腺病毒肌内免疫小鼠。免疫后三周，用100LD50的流感病毒A/PR/8/34(左)、A/FM/1/47(中)或70LD50的A/CA/04/09(右)对小鼠进行鼻内激发。显示了接种疫苗的小鼠的单独小鼠体重以及对照DPBS免疫小鼠的平均值和标准误差。人道地处死展现出显著的疾病体征且小于75％的基线体重的小鼠。
图3是mosaic和epigraph疫苗免疫原构建的示意图。该图显示了如何构建新型mosaic流感蛋白。将所有内部亚型血凝素(HA)或神经氨酸酶(NA)蛋白进行比对并用作天然序列以创建Mosaic序列。随机选择两个序列并创建两点重组(步骤1)。评价新的重组体，并且如果“适合性”增加，则将其放回蛋白质池中(步骤2)。该过程重复限定的世代数，通常是数百万，以创建一组模拟天然衍生的新型重组体的mosaic蛋白(步骤3)。随着过程的继续，选择具有最佳覆盖度的蛋白质，最终产生最适合的Mosaic蛋白质(步骤4)。最终，Mosaic疫苗免疫原在系统发育树上中心化，并平等地代表所有野生型蛋白，且偏向潜在的T和B细胞表位(PTBE)。
图4显示了五种不同疫苗免疫原方法的疫苗覆盖度。蓝色圆圈表示疫苗保护作用的预测覆盖度，并且红色圆圈表示由每种疫苗免疫原诱导的完全消除性免疫的预测覆盖度。基于野生型免疫原的疫苗具有很强的毒株特异性，并且产生很小的(如果有的话)交叉保护性免疫(A)。中心化共有序列疫苗被设计为定位至系统发育树的中心，并平等地代表所有野生型毒株(B)。我们已经发现与野生型基因相比，当疫苗不匹配时，这些疫苗免疫原的确提供了更大的交叉保护性免疫。第二种共有序列方法是创建共有独特序列(COUS)，其赋予了对所有独特的流感变体相等的权重，并偏向更现代的毒株(C)。另一种免疫原方法显示为mosaic免疫原。所述mosaic疫苗方法使用超过数百万世代的计算重组来选择单一最佳疫苗免疫原，与所有野生型序列的平均值相比，所述单一最佳疫苗免疫原具有最大≥9-mer覆盖范围。单一mosaic免疫原也定位在树的中心附近，因为它们被设计为代表所有已知的野生型序列(D)。epigraph免疫原的设计方式与mosaic免疫原相同，不同之处在于前者是一种多免疫原方法。所述epigraph设计的免疫原聚焦于蛋白质序列簇，以创建免疫原混合物，从而在所述混合物中要求的指定数量的免疫原中产生最大水平的覆盖度。本文描述的设计聚焦于使用三免疫原混合物以给出最大水平的覆盖度而没有过多序列的负担(E)。
图5显示了由共有序列中心化树中心(COT)、共有独特序列(CoUS)、Mosaic(Mos)和Epigraph(Epi)计算方法创建的H1 HA蛋白的系统发育分析。下载7313种独特的人H1流感病毒蛋白，并且排除实验室毒株和重复序列。为了显示序列在系统发育树上的位置，仅选择了显示出从1918至2018年序列进化的代表性序列。通过HIV数据库工具套件可得到的Mosaic疫苗设计和Epigraph程序分别用于创建Mosaic和Epigraph候选疫苗蛋白。共有序列COT疫苗蛋白被设计为定位至树的中心。使用Consensus Maker软件计算得到共有序列CoUS候选疫苗蛋白。CoUS和Mosaic蛋白两者都定位至主要的流感H1簇的中心，其代表H1多样性的大部分。这两种候选疫苗免疫原代表所有未来流感毒株将从其进化的普通或祖先蛋白。所述方法的两个主要例外是共有序列蛋白仅代表每个位置处最保守的氨基酸序列，而mosaic还试图代表最常见的重复序列，并且它也偏向≥9-mer基序，这最大程度地优化了潜在的T和B细胞表位(PTBE)。epigraph软件还被设计为最大程度地提高最终疫苗候选物内的PTBE，然而，它还被设计为鉴定最大程度地覆盖流感簇内最广范围的多样性的多个Epigraph蛋白序列。实际上，Epigraph 2蛋白也定位至所分析的所有人流感H1N1病毒的主要簇，而Epigraph1蛋白定位至pd09的中心区域。为了最大化簇之间的序列的覆盖度，创建了第三Epigraph 3蛋白。该第三蛋白是一种独特的蛋白质重组体，其中短片段被合成重组以制备蒙太奇蛋白序列，其代表经典和pd09祖先的病毒。共有序列蛋白、Mosaic蛋白和Epigraph蛋白分别以绿色、蓝色和黄色显示。FluZone蛋白以红色显示。激发病毒和用于免疫相关的病毒分别以紫色和黑色显示。
图6是显示对若干种HA疫苗候选物的总T细胞应答的图。为了确定所提出的策略是否会诱导更高水平的T细胞应答，将共有序列CoUS和Mosaic疫苗策略的能力与两种野生型HA疫苗的能力进行比较。将所有四种HA疫苗免疫原克隆至腺病毒表达系统中，并用108vp/小鼠免疫5只小鼠的组。免疫后三周，收获脾细胞并使用重叠的肽文库确定对四种相异流感病毒的总T细胞应答。使用ELISpot测定检测干扰素-γ分泌细胞。绿色条指示疫苗和肽库匹配。
图7是显示疫苗的表位作图的示意图。针对诱导的T细胞表位，使用布里斯班/59/07肽文库筛选来自接种Mosaic、CoUS、PR8和pd09 HA的小鼠的脾细胞，并且阳性肽分别由红色、绿色、紫色和黄色条表示。
图8是疫苗载体的蛋白质印迹分析。分析腺病毒疫苗载体的蛋白质表达。用相同的感染单位感染293细胞，并通过用多克隆山羊抗A/猪/印第安纳州/0392/2011血清探测印迹来检测蛋白质。
图9显示了本文所述的疫苗基因与人甲型流感H1的各种毒株之间的系统发育关系。
图10显示了本文所述的疫苗基因与人甲型流感H2的各种毒株之间的系统发育关系。
图11显示了本文所述的疫苗基因与人甲型流感H3的各种毒株之间的系统发育关系。
图12显示了本文所述的疫苗基因与人甲型流感H3的各种毒株之间的系统发育关系。
图13显示了本文所述的疫苗基因与人甲型流感H5的各种毒株之间的系统发育关系。
图14显示了本文所述的疫苗基因与人甲型流感N1的各种毒株之间的系统发育关系。
图15显示了本文所述的疫苗基因与人甲型流感N2的各种毒株之间的系统发育关系。
图16显示了本文所述的疫苗基因与人乙型流感HA维多利亚的各种毒株之间的系统发育关系。
图17显示了本文所述的疫苗基因与人乙型流感HA山形的各种毒株之间的系统发育关系。
图18显示了本文所述的疫苗基因与人乙型流感NA维多利亚的各种毒株之间的系统发育关系。
图19显示了本文所述的疫苗基因与人乙型流感NA山形的各种毒株之间的系统发育关系。
图20显示了本文所述的疫苗基因与猪甲型流感H1的各种毒株之间的系统发育关系。
图21显示了本文所述的疫苗基因与猪甲型流感H3的各种毒株之间的系统发育关系。
图22显示了本文所述的疫苗基因与猪甲型流感N1的各种毒株之间的系统发育关系。
图23显示了本文所述的疫苗基因与猪甲型流感N2的各种毒株之间的系统发育关系。
图24A显示了所有独特的H1 HA蛋白序列的邻接系统发育树，其中标记了代表性毒株。大流行2009(pdm09)毒株以深蓝色显示。
图24B是提交给SWISS-MODEL服务器并使用PyMOL软件可视化以预测每种免疫原的蛋白质折叠的HA氨基酸序列的示意图。显示了mosaic(蓝色)和野生型比较物HA(灰色和紫色)的头(深色)和茎(浅色)区域的预测结构，以及叠加所有三个HA结构的合并图像。
图24C是蛋白质印迹，其显示了在重组腺病毒5型中Mosaic和两个野生型比较物HA序列(A/PR/8/34和A/TX/05/09)的表达。
图25A-图25E是显示向小鼠单次接种流感毒株A/CA/07/09(25A)、A/NC/20/99(25B)、A/PR/8/34(25C)、A/WS/33(25D)和A/FM/1/47(25E)后的血凝抑制(HI)滴度的图。数据表示为平均值与标准误差(SEM)。
图25F是HI滴度应答的热图，其报告了疫苗组与病毒组之间的相应相似性百分比(****p<0.0001，***p<0.001；单因素方差分析和邦费罗尼(Bonferroni)多重比较)。
图26A-图26E是显示向小鼠接种流感毒株A/CA/07/09(26A)、A/NC/20/99(26B)、A/PR/8/34(26C)、A/WS/33(26D)和A/FM/1/47(26E)并加强后的HI滴度的图。
图26F是HI滴度响应的热图，其报告了疫苗组与病毒组之间的相应相似性百分比。数据表示为平均值与标准误差(SEM)。(****p<0.0001，***p<0.001，**p<0.01；单因素方差分析和邦费罗尼多重比较)。
图27A-图27D是显示单次接种流感毒株A/CA/04/09(27A)、A/PR/8/34(27B)、A/NC/20/99(27C)和A/布里斯班/59/07(27D)后的抗体应答的图。显示了OD450处的平均吸光度与标准误差(SEM)(****p<0.0001，**p<0.01，*p<0.05，n.s.不显著；单因素方差分析和邦费罗尼多重比较)。
图28A-图28D是显示单次接种A/加利福尼亚州/07/09(28A)、A/波多黎各/8/34(28B)、A/新喀里多尼亚/20/99(28C)或A/布里斯班/59/07(28D)毒株后的总T细胞应答的图。数据表示为每百万个脾细胞中平均斑点形成细胞(SFC)的数量与标准误差(SEM)(****p<0.0001，***p<0.001，**p<0.01，*p<0.05；单因素方差分析和邦费罗尼多重比较)。
图29A是关于HA基因定位显示的阳性应答(即，大于每百万个脾细胞中50个斑点形成细胞(SFC))的示意图。
图29B-图29D是显示对于A/布里斯班/59/07(29B)、A/新喀里多尼亚/20/99(29C)和A/加利福尼亚州/07/09(29D)的阳性肽的定量的图，所述阳性肽在HA序列上示意性地示出以指示可能的表位的位置。数据表示为每百万个脾细胞中平均斑点形成细胞(SFC)与标准误差(SEM)。
图30是显示针对A/加利福尼亚州/04/09致死激发的保护作用的图。单独小鼠的体重减轻显示在(30A、30B)中，平均体重减轻显示在(30C、30D)中，并且存活率百分比显示在(30E、30F)中。(与PBS对照相比，用对数秩检验分析存活率数据；****p<0.0001，***p<0.001，**p<0.01，*p<0.05)。
图31是显示分别用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫并在3周后用100MLD50激发的小鼠的单独小鼠的体重减轻(31A、31B)、平均体重减轻(31C、31D)和存活率百分比(31E、31F)的图。用对数秩检验分析与PBS对照相比的存活率数据；*p<0.05。
图32是显示分别用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫并在3周后用100MLD50激发的小鼠的单独小鼠的体重减轻(32A、32B)、平均体重减轻(32C、32D)和存活率百分比(32E、32F)的图。与PBS对照相比，用对数秩检验分析生存数据(**p<0.01，*p<0.05)。
图33是展示针对A/南昌/1/99致死激发的保护作用的图。单独小鼠的体重减轻显示在(33A、33B)中，平均体重减轻显示在(33C、33D)中，并且存活率百分比显示在(33E、33F)中。(与PBS对照相比，用对数秩检验分析存活率数据；****p<0.0001，***p<0.001，**p<0.01，*p<0.05)。
图34A显示了由本文所述的Mosaic和三种Epigraph免疫原与原始序列群体的比对构建的Jukes-Cantor邻接树。
图34B是来自每种载体的血凝素表达的蛋白质印迹。使用野生型比较物HA基因(A/猪/得克萨斯州/4199-2/1998[TX98])作为对照。
图35A显示了疫苗接种和加强的实验时间线。
图35B显示了被选择用于分析抗体发展的代表性相异H3猪流感病毒。
图35C是如世界卫生组织标准方案所描述的进行血凝抑制(HI)的图。HI滴度报告为几何平均滴度±标准误差。虚线表示标准滴度或40，其被认为提供针对激发的保护作用。
图36A是显示由针对四种代表性相异swH3病毒的疫苗免疫原诱导的T细胞表位的示意图。如果斑点形成细胞(SFC)/百万大于50，则认为肽为阳性。
图36B是显示对阳性肽的单独应答的图。
图36C是显示总T细胞应答的图。
图37A是显示用代表性人H3N2病毒分离株确定的几何平均HI滴度±标准误差的图。
图37B是显示被认为对针对四种代表性人流感毒株中的任一种的交叉反应性T细胞免疫应答呈阳性的肽的示意图。
图37C是显示对阳性肽的单独应答的图。
图37D是显示总T细胞应答的图。
图38A是显示用曼尼托巴省/2005激发后小鼠体重减轻的图。
图38B是显示用科罗拉多州/1977激发后小鼠体重减轻的图。
序列表
SEQ ID NO:1是使用共有独特序列(COUS)产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:2是使用共有独特序列(COUS)产生的来自人H1(pH1N1)甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:3是使用Mosaic1产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:4是使用Mosaic 2产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:5是使用Mosaic 3产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:6是使用Epigraph1产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:7是使用Epigraph2产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:8是使用Epigraph3产生的来自人H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:9是使用COUS产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:10是使用Mosaic1产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:11是使用Mosaic2产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:12是使用Mosaic3产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:13是使用Epigraph1产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:14是使用Epigraph2产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:15是使用Epigraph3产生的来自人H2甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:16是使用COUS产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:17是使用Mosaic1产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:18是使用Mosaic2产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:19是使用Mosaic3产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:20是使用Epigraph1产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:21是使用Epigraph2产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:22是使用Epigraph3产生的来自人H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:23是使用COUS产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:24是使用Mosaic1产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:25是使用Mosaic2产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:26是使用Mosaic3产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:27是使用Epigraph1产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:28是使用Epigraph2产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:29是使用Epigraph3产生的来自人H5甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:30是使用COUS产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:31是使用Mosaic1产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:32是使用Mosaic2产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:33是使用Mosaic3产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:34是使用Epigraph1产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:35是使用Epigraph2产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:36是使用Epigraph3产生的来自人N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:37是使用COUS产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:38是使用Mosaic1产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:39是使用Mosaic2产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:40是使用Mosaic3产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:41是使用Epigraph1产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:42是使用Epigraph2产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:43是使用Epigraph3产生的来自人N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:44是使用COUS产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:45是使用中心共有序列(CC)产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:46是使用Mosaic1产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:47是使用Mosaic2产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:48是使用Mosaic3产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:49是使用Epigraph1产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:50是使用Epigraph2产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:51是使用Epigraph3产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:52是使用Epigraph4产生的来自人维多利亚类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:53是使用COUS产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:54是使用Mosaic1产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:55是使用Mosaic2产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:56是使用Mosaic3产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:57是使用Epigraph1产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:58是使用Epigraph2产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:59是使用Epigraph3产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:60是使用Epigraph4产生的来自人山形类乙型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:61是使用COUS产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:62是使用Mosaic1产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:63是使用Mosaic2产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:64是使用Mosaic3产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:65是使用Epigraph1产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:66是使用Epigraph2产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:67是使用Epigraph3产生的来自人维多利亚类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:68是使用COUS产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:69是使用Mosaic1产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:70是使用Mosaic2产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:71是使用Mosaic3产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:72是使用Epigraph1产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:73是使用Epigraph2产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:74是使用Epigraph3产生的来自人山形类乙型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:75是使用COUS得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:76是使用Mosaic1得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:77是使用Mosaic2得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:78是使用Mosaic3得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:79是使用Epigraph1得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:80是使用Epigraph2得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:81是使用Epigraph3得到的来自猪H1甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:82是使用COUS得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:83是使用Mosaic1得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:84是使用Mosaic2得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:85是使用Mosaic3得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:86是使用Epigraph1得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:87是使用Epigraph2得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:88是使用Epigraph3得到的来自猪H3甲型流感血清型的通用HA疫苗的氨基酸序列。
SEQ ID NO:89是使用COUS得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:90是使用Mosaic1得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:91是使用Mosaic2得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:92是使用Mosaic3得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:93是使用Epigraph1得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:94是使用Epigraph2得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:95是使用Epigraph3得到的来自猪N1甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:96是使用COUS得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:97是使用Mosaic1得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:98是使用Mosaic2得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:99是使用Mosaic3得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:100是使用Epigraph1得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:101是使用Epigraph2得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:102是使用Epigraph3得到的来自猪N2甲型流感血清型的通用NA疫苗的氨基酸序列。
SEQ ID NO:103是使用共有序列(Cons)得到的来自人H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:104是使用Mosaic1得到的来自人H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:105是使用Epigraph1得到的来自人H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:106是使用Epigraph2得到的来自人H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:107是使用Epigraph3得到的来自人H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:108是使用共有序列(Cons)得到的来自人H3甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:109是使用Mosaic1得到的来自人H3甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:110是使用Epigraph1得到的来自人H3甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:111是使用Epigraph2得到的来自人H3甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:112是使用Epigraph3得到的来自人H3甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:113是使用共有序列得到的来自猪H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:114是使用Mosaic1得到的来自猪H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:115是使用Epigraph1得到的来自猪H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:116是使用Epigraph2得到的来自猪H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:117是使用Epigraph3得到的来自猪H1甲型流感的编码通用HA疫苗的核酸序列。
SEQ ID NO:118是使用共有序列得到的来自猪H3甲型流感的编码通用NA疫苗的核酸序列。
SEQ ID NO:119是使用Mosaic1得到的来自猪H3甲型流感的编码通用NA疫苗的核酸序列。
SEQ ID NO:120是使用Epigraph1得到的来自猪H3甲型流感的编码通用NA疫苗的核酸序列。
SEQ ID NO:121是使用Epigraph2得到的来自猪H3甲型流感的编码通用NA疫苗的核酸序列。
SEQ ID NO:122是使用Epigraph3得到的来自猪H3甲型流感的编码通用NA疫苗的核酸序列。
具体实施方式
通用流感疫苗已成为一项高度优先事项，并且为实现这一目标正在进行若干努力，因为预防人类流感将对医疗保健成本和负担产生非常重大的影响。在此，提出了一种使用先前未采用过的若干种策略来创建通用病毒疫苗的解决方案。因此，本文所述的方法是独特的，因为它利用了感染人和猪的所有已知毒株，从而本文所述的基因是独特的并且在自然界中不存在。
三价灭活疫苗(TIV)(例如，FluZone)是现代流感疫苗接种的主力，并已保护了数百万人免于流感的发病和死亡。虽然有效，但TIV对于支持供替代的疫苗平台的开发具有局限性。例如，在含胚卵中产生疫苗是该疫苗平台的一个基本问题。很大一部分人对卵白蛋白(egg ovalbumin)过敏，并且不能接种该疫苗。在卵中产生流感疫苗耗费时间，劳动强度大，并且依赖于含胚卵的可用性。TIV仅提供短期免疫，并且这种免疫具有高度的毒株特异性。肌内递送不刺激高水平的分泌型IgA，后者被认为对异源病毒更具反应性，并且重要的是，TIV也无法诱导交叉保护性T细胞免疫。
减毒的冷适应活疫苗(例如，FluMist)是一种冷适应流感病毒平台，当在温度低于37℃的情况下鼻内引入时，其可能复制失败。为此，FluMist可以优于TIV，因为它可以驱动抗体和T细胞应答的组合。冷适应的优点是更快的疫苗生产，诱导保护作用，并具有同时产生粘膜和体液免疫应答的能力，所述粘膜和体液免疫应答包括分泌型IgA、血清IgG、细胞因子刺激和CTL应答的引发。所述平台受限于免疫持续时间短、毒株特异性以及对非常年轻、老年或免疫功能低下的人禁用。
亚洲和澳大利亚的流感监测被用来预测下一年哪些流感毒株可能在美国传播。这种普遍有效的策略可能会失败。实际上，在1997与2005年之间WHO推荐的毒株与引起暴发的那些毒株的一致性只有50％。换句话说，在那些流感季节中，毒株预测有50％都失败了，这使人类无法被保护免于季节性和潜在大流行性流感。基于HA氨基酸差异(20.5％)，疫苗与2009大流行性猪流感的不匹配程度在所有H1毒株之间接近最大约21％HA氨基酸差异。作为这种不匹配的结果，2009大流行感染了全球人口的24％。相比之下，HA1-COT免疫原与大流行毒株仅7.6％差异，并在小鼠激发模型中提供保护作用。
本文所述的疫苗策略对于流感的预防将是重要的，并且将显著降低或消除来自猪的突发人畜共患的重新组合的流感病毒的可能性。
与野生型基因相比，中心化疫苗基因已显示出针对相异病毒毒株的更广泛的交叉反应性免疫，并且作为通用疫苗可能非常有用。这些中心化疫苗基因可以通过若干种不同的方法构建。本文评价了使用共有序列、Mosaic和Epigraph方法设计的疫苗基因作为通用疫苗基因诱导针对相异H1N1和H3N2猪甲型流感病毒的保护作用的能力。
以下方法用于创建本文所述的通用疫苗免疫原：
中心化共有序列(CC)基因-使用代表性野生型基因序列的比对，在蛋白质的每个位置处使用最常见的氨基酸来构建CC基因。该策略创建了这样的疫苗基因，其定位至系统发育树的中心并且与所有已知的野生型病毒毒株等距。参见图4B。由于功能性元件(如切割位点、跨膜结构域、定位信号和融合结构域)对天然蛋白质至关重要，因此它们被保存在CC基因中。与野生型基因相比，该技术已显示出对相异HIV和流感病毒具有更大的交叉反应性免疫。
共有独特序列(COUS)基因-COUS基因与CC基因相似，因为它也在每个位置处使用最常见的氨基酸。然而，用于创建COUS基因的比对由给定基因的所有已知独特序列组成。该技术涵盖了数据库中所有已知的变体，并且偏向大多数。图4C。该技术的最大优点是，它选择了与主要循环毒株最密切相关的疫苗基因，因此更有可能针对随机选择的野生型激发毒株具有保护作用。
Mosaic基因-使用计算机重组产生Mosaic基因。全长天然序列被重复重组，并优先考虑序列中9-mer重复。该策略使用遗传算法来选择最大可能的潜在T细胞表位(PTE)。图4D。Mosaic基因已显示是对于HIV和其他病原体(包括HCV、埃博拉和流感)的有希望的疫苗候选物。Fischer等人,2007,Nature Med.,13:100-6。
Epigraph基因-Mosaic和Epigraph基因被设计为提供相同的PTE优化，但是Epigraph基因比Mosaic和共有序列方法具有明显更大的优点。与使用算法的Mosaic方法不同，Epigraph是使用更快的基于图的设计来设计的。Epigraph方法的主要要素是设计多个Epigraph基因从而最大化疫苗覆盖度的能力。图4E。所述多个Epigraph基因可以定位至天然蛋白质的主要系统发育簇，或者可以跨越使用重组Epigraph基因的多个簇之间的覆盖度。该方法已用于开发个性化的治疗性HIV疫苗候选物和泛丝状病毒疫苗。Theiler和Korber,2018,Stat.Med.,37:181-94。
有人提出，中心化抗原将诱导针对不匹配的流感激发的最大程度的交叉保护性免疫应答，并且如果是这样的话，它们将对流感疫苗研究领域产生重大影响。对流感疫苗接种的临床实践的改变的例子可以包括在年度三价注射疫苗(TIV)中使用中心化抗原或储存中心化疫苗以用于疫苗不匹配的情况。重复暴露于中心化疫苗(如果使用的话，例如在年度配制中使用)可能诱导由于重复加强所致的增强的交叉保护性应答以及对中心化抗原中保守表位的亲和力成熟。
我们创建中心化共有序列流感基因的方法是独特的。对于共有序列HA抗原，在数据库中鉴定出独特的序列。这消除了可能使共有序列抗原偏向更常见毒株的重复序列，并允许重建代表所有平等进化的序列的中心化序列。基于小鼠的初步数据，将中心化基因包含在猪流感疫苗中将降低完全疫苗不匹配的风险。另外，中心化基因可以加强对该亚型中所有流感毒株共有的保守元件的应答。
本文描述的COUS基因也是独特的，因为它们是使用由所有已知且独特的流感基因组成的数据集创建的。该策略使这些基因偏向主要的循环毒株。因此，优化了COUS基因与循环病毒的遗传关系。该策略增加了疫苗效力的可能性。
为了创建Mosaic猪流感疫苗抗原，使用了来自洛斯阿拉莫斯国家实验室工具套件(Los Alamos National Laboratories Tool Suite)的Mosaic疫苗设计器程序。此外，从流感研究数据库获得了独特的猪流感HA蛋白序列，并通过计算分析了其最佳的潜在T细胞表位覆盖度。
最后，将相同的HA数据集提交给Epigraph软件套件，在其中创建三种HA抗原混合物。Epigraph软件选择最能代表数据集中最常见的B和T细胞表位的免疫原。该免疫原被命名为Epigraph1。第二种免疫原被设计为代表第二种最常见的B和T细胞表位，称为Epigraph2。最后，设计了代表第三种最常见的B和T细胞表位的第三种免疫原，并将其命名为Epigraph3。
对所有疫苗抗原进行了系统发育分析，并且发现在中心化共有序列蛋白和Mosaic蛋白的情况下其定位至系统发育树的中心，或者在Epigraph疫苗的情况下定位至两个主要流感簇，其中重组体覆盖较小簇(图4)。
本文所述的方法可以应用于具有高水平遗传多样性的其他病毒(例如，人免疫缺陷病毒(HIV)、新城鸡瘟病毒(NDV)、猪繁殖与呼吸综合征(PRRS)或人乳头瘤病毒(HPV))。尽管本文所述的中心化流感基因可以用作人疫苗，但是可以应用这些相同的策略向宿主动物(例如，猪、家禽、马)接种疫苗以抵抗流感感染，并且显著降低或消除出现重新组合的猪流感病毒的可能性。因此，向在病毒进化的源头的宿主动物接种疫苗可以在这一初级步骤中进行干预，并且导致消除引起大流行的潜在的未来人畜共患流感暴发。
产生了许多不同的疫苗多肽；此类多肽的氨基酸序列如SEQ ID NO:1-102所示，并且代表性核酸序列如SEQ ID NO:103-122所示。本领域技术人员将理解，可以容易地确定编码每个氨基酸序列的核酸序列。
除了具有SEQ ID NO:1-102所示的序列并且由SEQ ID NO:103-122所示的核酸编码的疫苗多肽之外，还提供了如下多肽和核酸，所述多肽和核酸与具有SEQ ID NO:1-102所示的序列的疫苗多肽或者与SEQ ID NO:103-122所示的核酸具有至少90％序列同一性(例如，至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、至少99％或100％序列同一性)。
在计算序列同一性百分比时，将两个序列进行比对并测定这两个序列之间核苷酸或氨基酸残基的相同匹配的数量。相同匹配的数量除以比对区域的长度(即比对的核苷酸或氨基酸残基的数量)并乘以100以得出序列同一性百分比的值。应当理解，所述比对区域的长度可以是其中一个序列或两个序列的一部分，至多是最短序列的全长大小。还应理解的是，单个序列可以与多于一个其他序列进行比对，并且因此可以在每个比对的区域上具有不同的序列同一性百分比的值。
可以使用可在万维网(World Wide Web)上ncbi.nlm.nih.gov处获得的已并入BLAST(基本局部比对搜索工具)程序中的由Altschul等人(1997,Nucleic Acids Res.,25:3389 3402)描述的算法进行两个或更多个序列的比对以测定序列同一性百分比。可以进行BLAST搜索以测定使用Altschul等人算法比对的序列(核酸或氨基酸)与任何其他序列或其部分之间的序列同一性百分比。BLASTN是用于比对和比较核酸序列之间的同一性的程序，而BLASTP是用于比对和比较氨基酸序列之间的同一性的程序。当利用BLAST程序计算序列与另一个序列之间的同一性百分比时，通常使用相应程序的默认参数。
还提供了含有编码多肽的核酸分子的载体。载体(包括表达载体)可以商购获得或可以通过重组技术产生。含有核酸分子的载体可以具有与这种核酸分子可操作地连接的用于表达的一个或多个元件，并且还可以包括序列(如编码可选择标记的那些(例如，抗生素抗性基因)和/或可用于纯化多肽的那些(例如，6xHis标签))。用于表达的元件包括指导和调节核酸编码序列的表达的核酸序列。表达元件的一个例子是启动子序列。表达元件还可以包括调节核酸分子的表达的内含子、增强子序列、应答元件或诱导型元件中的一种或多种。表达元件可以是细菌、酵母、昆虫、哺乳动物或病毒来源的，并且载体可以含有来自不同来源的表达元件的组合。如本文所用，可操作地连接意指用于表达的元件以这样的方式相对于编码序列位于载体中，所述方式使得指导或调节所述编码序列的表达。
可以将核酸分子(例如，载体(例如，表达载体、病毒载体)中的核酸分子)引入宿主细胞中。术语“宿主细胞”不仅指核酸分子已被引入其中的一个或多个特定细胞，而且还指这种细胞的后代或潜在后代。许多合适的宿主细胞是本领域技术人员已知的；宿主细胞可以是原核细胞(例如，大肠杆菌)或真核细胞(例如，酵母细胞、昆虫细胞、植物细胞、哺乳动物细胞)。代表性宿主细胞可以包括但不限于源自哺乳动物(包括人、猴子、小鼠、大鼠、兔子和仓鼠)的A549、WEHI、3T3、10T1/2、BHK、MDCK、COS 1、COS 7、BSC 1、BSC 40、BMT 10、VERO、WI38、HeLa、293细胞、Saos、C2C12、L细胞、HT1080、HepG2和原代成纤维细胞、肝细胞和成肌细胞。用于将核酸分子引入宿主细胞中的方法在本领域中是熟知的，并且包括但不限于磷酸钙沉淀、电穿孔、热休克、脂转染、显微注射和病毒介导的核酸转移(例如，转导)。
关于多肽，“纯化的”是指已经从其天然伴随的细胞组分分离或纯化的多肽(即，肽或多肽)。通常，当多肽以干重计为至少70％(例如至少75％、80％、85％、90％、95％或99％)、不含与其天然缔合的多肽和天然存在的分子时，所述多肽被认为是“纯化的”。由于化学合成的多肽从本质上与其天然伴随的组分分离，因此合成的多肽被认为是“纯化的”，但可以进一步从用于合成所述多肽的组分(例如，氨基酸残基)移取。关于核酸分子，“分离的”是指核酸分子与基因组中通常与其缔合的其他核酸分子分离。此外，分离的核酸分子可以包括工程化核酸分子，如重组或合成的核酸分子。
多肽可以通过已知的方法(如DEAE离子交换、凝胶过滤和/或羟基磷灰石色谱法)从天然来源(例如，生物样品)来获得(例如，纯化)。纯化的多肽也可以例如通过在表达载体中表达核酸分子或通过化学合成来获得。多肽的纯度的程度可以使用任何适当的方法(例如，柱色谱法、聚丙烯酰胺凝胶电泳或HPLC分析)来测量。类似地，可以使用常规方法获得(例如，分离)核酸分子，所述常规方法例如但不限于重组核酸技术(例如，限制酶消化和连接)或聚合酶链式反应(PCR；参见例如，PCR Primer:A Laboratory Manual,Dieffenbach&Dveksler,Eds.,Cold Spring Harbor Laboratory Press,1995)。此外，分离的核酸分子可以是化学合成的。
在一些实施方案中，如本文所述的疫苗可以是多价(例如，4价、12价、16价)的。例如，疫苗可以包括多个如本文所述的多肽。例如，疫苗可以包括一种或多种Mosaic1疫苗多肽(例如，SEQ ID NO:3、10、17、24、31、38、46、54、62、69、76、83、90、97、104、109、114或119)、一种或多种Epigraph1疫苗多肽(例如，SEQ ID NO:6、13、20、27、34、41、49、57、65、72、79、86、93、100、105、110、115或120)、一种或多种Mosaic2疫苗多肽(例如，SEQ ID NO:4、11、18、25、32、39、47、55、63、70、77、84、91或98)、一种或多种Epigraph2疫苗多肽(SEQ ID NO:7、14、21、28、35、42、50、58、66、73、80、87、94、101、106、111、116或121)或其组合。在一些实施方案中，疫苗可以包括一种或多种Epigraph疫苗多肽(例如，Epigraph1、Epigraph2、Epigraph3；例如，SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95或100-102)。
通常，将本文所述的一种或多种人疫苗多肽施用于人以提供针对流感的保护性免疫，并且将本文所述的一种或多种猪疫苗多肽施用于猪以提供针对流感的保护性免疫。然而，在一些情况下，可以将本文所述的一种或多种人疫苗多肽施用于动物(例如，鸟、猪、马、狗、猫)以提供针对流感(例如，禽流感、猪流感、马流感、犬流感、猫流感)的保护性免疫，和/或可以将本文所述的一种或多种猪疫苗多肽施用于人或非猪动物以提供针对流感的保护性免疫。
本文所述的疫苗多肽可以悬浮于生理学上可接受的或相容的载体中，并将其施用于受试者。药学上可接受的载体包括盐水，其可以用多种缓冲溶液(例如，磷酸盐缓冲盐水)、乳糖、蔗糖、磷酸钙、明胶、葡聚糖、琼脂、果胶和水来配制。常规的和药学上可接受的施用途径包括但不限于口服、鼻内、气管内、通过吸入、静脉内、肌内、眼内、皮下、皮内、经粘膜或通过其他施用途径直接递送至器官(例如像肝或肺)。可以使用病毒载体(例如，腺病毒、腺相关病毒、逆转录病毒、甲病毒、副粘病毒或弹状病毒)或纳米颗粒(例如，脂质纳米颗粒)递送本文所述的疫苗多肽或编码此类病毒多肽的核酸(例如，DNA、RNA)。
可以将本文所述的疫苗多肽以足以提供治疗益处而没有过度不利影响的量施用。施用于受试者的疫苗多肽的剂量将主要取决于诸如正在治疗的病症以及受试者的年龄、体重和健康等因素。简单地举例来说，待施用于人受试者的疫苗多肽的有效剂量可以在以下范围内：约15微克(μg)至约90μg(例如，约20μg至约80μg；约25μg至约75μg；约30μg至约70μg；约40μg至约60μg；约45μg至约55μg；约50μg)。在一些情况下，可以季节性地或在检测流感或针对流感的抗体时向受试者施用一种或多种本文所述的疫苗多肽；在一些情况下，可以在儿童早期向受试者施用一种或多种本文所述的疫苗多肽，从而在数年或数十年内在该受试者中提供保护性免疫。在某些情况下，可能需要在首次施用后的一段时间(例如，几周、几个月、几年(例如，几十年))之后以“加强”疫苗的形式向受试者再施用一种或多种本文所述的疫苗多肽。
根据本发明，在本领域技术范围内可以采用常规的分子生物学、微生物学、生物化学和重组DNA技术。在文献中对这些技术进行了充分的解释。将在以下实施例中进一步描述本发明，这些实施例不限制权利要求中所述的方法和物质组合物的范围。
实施例
实施例1-病毒基因和产生的疫苗序列
本文所述的疫苗基因与野生型循环基因之间的遗传关系如图9-图23所示。由于本文所述的疫苗基因是使用所有独特的HA序列制备的，因此它们不像使用其他策略(如共有序列、COBRA、Mosaics和COT)制备的任何基因。
本文所述的独特通用疫苗基因用于抵抗人甲型流感病毒(H1、H2、H3、H5、N1和N2((SEQ ID NO:1-43))、人乙型流感病毒(HA山形类、HA维多利亚类、NA山形类、NA维多利亚类(SEQ ID NO:44-74))和猪甲型流感病毒(H1、H3、N1和N2(SEQ ID NO:75-102))，并且可以应用于产生提供针对所有已知流感毒株的完全保护作用的通用流感疫苗。编码代表性流感疫苗的核酸序列如SEQ ID NO:103-122所示。
实施例2-初步结果
IACUC.根据实验室动物护理的评估与认可协会(Association for Assessmentand Accreditation of Laboratory Animal Care，AALAC)指南使用经相应UNL IACUC协议批准的动物使用协议，将所有动物安置在UNL Life Sciences Annex中。所有动物实验均按照动物福利法案(Animal Welfare Act)的规定、PHS动物福利政策(PHS Animal WelfarePolicy)、NIH实验动物护理和使用指南(NIH Guide for the Care and Use ofLaboratory Animals)的原则以及UNL的政策和程序进行。
性别作为生物变量。在体内研究中仅使用雌性小鼠。然而，所有成功的疫苗候选物都是在使用雄性小鼠的研究中证实的。
生物危害。所有生物危害材料都经过UNL机构生物安全委员会(InstitutionalBiosafety Committee，IBC)的批准。UNL IBC负责UNL实验室内感染原和重组DNA的安全使用。这项研究没有使用任何评级高于BSL2的试剂。
严密性和透明度。为了确保统计学意义，使用5只小鼠和雪貂的组。为了证实再现性，所有体内研究重复至少一次。此外，在每个实验中进行充当重复实验的初步剂量依赖性研究。
中心化COT H1、2、3和5基因的产生。通过比较从1933至2009年选择的H1 HA蛋白产生了系统发育树，其分支之间序列差异为约21.0％。由于高水平的遗传多样性，选择单个野生型HA蛋白作为广泛交叉反应性疫苗被认为是不可行的。在过去的78年中，并非选择一种野生型基因作为疫苗，而是产生了模拟流感感染祖先的中心化基因。其基本原理是产生相对于所有其他变体定位在中心的免疫原。与任意两种随机选择的蛋白质相比，这种蛋白质与所有变体实际上具有更低的序列差异。已发现中心化基因在BALB/c小鼠中诱导针对相异流感毒株的非常有效的保护水平。
中心化HA基因的系统发育分析。共有序列COT基因与其他野生型基因的关系如图1所示。所有共有基因都定位至相应树中每一个的中心区域。H1、H2、H3和H5共有基因与野生型基因的平均遗传距离分别为7.04％、2.86％、5.44％和1.65％。然而，野生型H1、H2、H3和H5基因之间的最大遗传距离分别为20.7％、7.9％、14.7％和5.1％。中心化COT免疫原的作用是通过与所有分析的野生型基因等距来降低不匹配基因的遗传多样性(图1)。中心树显示出流感HA亚型之间的显著差异。流感的进化允许HA基因的极端水平的可塑性，并且HA亚型可以多达60％相异并且仍然具有相同的功能(图1)。
在不匹配的情况下，与野生型免疫原相比，H1-COT诱导了更好的保护作用。
针对致死A/PR/8/34流感的保护作用。与不匹配疫苗H1-COT和A/TX/05/09相比，同源PR/8/34疫苗能够使用最低剂量的疫苗诱导保护性应答(图2A)。A/PR/8/34免疫小鼠在剂量低至107vp的情况下未显示疾病或死亡的任何体征(图2A)。然而，接种H1-COT疫苗的小鼠从激发中恢复并且存活，而用相同剂量的A/TX/05/09接种的小鼠中60％没有存活(图2A)。
针对致死A/FM/1/47流感的保护作用。在这项激发研究中，所有疫苗都与激发毒株不匹配。接种H1-COT疫苗的小鼠显示出最小的体重减轻和疾病以及针对致死激发显示出最高水平的存活率(图2B)。中心化H1-COT疫苗优于两种野生型疫苗免疫原。
针对致死A/加利福尼亚州/04/09的保护作用。匹配的CA/04/09疫苗针对A/CA/04/09致死激发提供了最佳保护水平。然而，当疫苗不匹配时，与A/PR/8/34相比，中心化H1-COT诱导更好的保护性免疫(图2C)。重要的是，接种H1-COT的小鼠中60％存活，而接种A/PR/8/34的小鼠中仅20％存活(图2C)。
这些数据证明，中心化HA1-con免疫原能够在包括2009H1N1在内的三种单独的严格激发模型中介导针对相异流感病毒的交叉保护作用。
该项目将测试中心化免疫原作为基础免疫原针对其他流感毒株的效用。
在多价(H1+H2+H3+H5)COT HA疫苗接种后针对相异致死激发的保护作用。疫苗效力的最终证明是一项激发研究。向各组小鼠接种由四种不同的共有序列COT HA亚型H1、H2、H3和H5组成的多价疫苗。已成功地显示，多种HA免疫原可以同时递送并且中心化免疫原针对代表H1N1、H3N1、H3N2和H5N1流感的9种相异致死流感病毒中的8种提供了100％存活率(表1)。该激发模型非常严格，并且七种激发由100小鼠致死剂量50％(MLD50)组成且两种激发是在10MLD50下。另外，在9种致死激发中7种没有疾病体征。这与用传统流感疫苗FluMist和FluZone免疫的小鼠形成了鲜明对比。用这些疫苗免疫的小鼠针对所测试的7种流感病毒中的7种诱导了稳健的HI滴度，然而，当用致死流感病毒激发时，它们未能提供任何针对死亡疾病的保护作用(表1)。有趣的是，与传统疫苗相比，通用多价疫苗还诱导了显著更高的T细胞免疫和中和抗体。在设计和产生这些通用疫苗免疫原的过程中，可以选择提高HA-COT疫苗的细胞和体液免疫。
表1.支持数据的概述
Figure GDA0003214791590000261
Figure GDA0003214791590000271
人H1和H3 COT、CoUS、Mosaic和Epigraph候选疫苗免疫原的构建。野生型疫苗免疫原具有不佳的免疫原性，并且提供毒株特异性免疫(图4A)。已经评价了树中心(COT)策略，其中疫苗免疫原定位至系统发育树的中心节点，并且因此与所有分析的变体等距。这是有利的，因为与任何随机野生型基因相比，COT免疫原最有可能与疫苗不匹配更密切相关(图4B)。这种方法的主要缺点是它赋予树的所有主要分支相等的权重，其中一些分支只有一个或两个独特的毒株。第二种方法仅使用独特序列来创建共有序列免疫原，即共有独特序列(CoUS)。该方法的优点是赋予所有已知的流感变体相等的权重，并且偏向于更现代的毒株。该策略用于定位疫苗免疫原，以针对最大数量的独特序列提供最大保护作用(图4C)。第三种通用疫苗策略使用针对于代表最常见的重复基序的重组体而选择的算法进行计算机重组。这种Mosaic策略描述在图3中，并且具有选择通用疫苗免疫原的优点，所述通用疫苗免疫原已针对最大数量的T和B细胞表位进行了优化(图4D)。最终策略利用与Mosaic策略相同的T和B细胞表位的优化，但是在这种情况下，选择针对最大化疫苗覆盖度的疫苗免疫原混合物(图4E)。
用于创建人H1候选疫苗免疫原的参数。为了创建中心化人流感H1 HA基因，从流感研究数据库下载6908种独特人HA蛋白序列(排除重复序列)。这些序列是全长的，并且代表了从1918至2018年的全球覆盖范围。如前所述创建共有序列COT序列(Weaver等人,2011,PLoS One,6:e18314)。为了创建共有序列CoUS基因，仅将独特序列提交给Consensus Maker软件程序。为了产生Mosaic基因，将FASTA格式化序列提交给Los Alamos国家实验室的Mosaic疫苗设计器，并使用以下参数创建Mosaic序列：混合物大小：1，表位长度：9，稀有阈值：1，运行时间：10h，群体大小：200，循环时间：10，延迟时间：10，内部交叉概率：0.5。Mosaic疫苗序列覆盖度：0.730394。使用具有以下参数的Epigraph疫苗设计软件创建Epigraph序列：算法：未比对序列，表位长度：9，混合物大小：3。将共有序列COT和CoUS以及优化的基因引入回原始蛋白质序列文件中，在ClustalW中重新比对，并使用PAUP 4.0a利用nexus输出文件生成邻接树。鉴定中心化蛋白质与天然蛋白质的系统发育关系。
用于创建人H3候选疫苗免疫原的参数。为了创建共有序列和优化基因，从流感研究数据库下载7313种独特人流感H3 HA蛋白序列。这些序列是全长的，并且代表了从1918至2018年的全球覆盖范围。如前所述创建共有序列COT(Weaver等人,2011,PLoS One,6:e18314)。使用Consensus Maker软件利用独特H3 HA蛋白序列创建CoUS序列。将FASTA格式化序列提交给Los Alamos国家实验室的Mosaic疫苗设计器，并且使用以下参数创建mosaic序列：混合物大小：1，表位长度：9，稀有阈值：1，运行时间：10h，群体大小：200，循环时间：10，延迟时间：10，内部交叉概率：0.5。Mosaic疫苗序列覆盖度：0.827791。使用具有以下参数的Epigraph软件创建Epigraph序列：算法：未比对序列，表位长度：9，混合物大小：3。将中心化基因引入回原始蛋白质序列文件中，在ClustalW中重新比对，并且使用PAUP 4.0a利用nexus输出文件生成邻接树。鉴定中心化蛋白质与天然蛋白质的系统发育关系。
对新型HA疫苗免疫原的改善的细胞免疫应答。用表达新型人H1 Mosaic和CoUS基因的腺病毒免疫小鼠，并且将其针对四种相异流感病毒驱动细胞免疫的能力进行比较。当使用重叠肽文库和ELISpot测定进行筛选时，发现在所有不匹配的情况下，新的Mosaic和CoUS基因诱导了更高水平的整体细胞免疫(图6)。有趣的是，Mosaic和CoUS基因针对A/波多黎各/8/34肽诱导的T细胞免疫等同于表达同源PR8 HA免疫原的疫苗的T细胞免疫。在pd09免疫应答的情况下，同源HA疫苗针对同源肽诱导了最稳健的免疫。然而，在疫苗不匹配的情况下，与野生型PR8和pd09比较物疫苗相比，Mosaic和CoUS HA疫苗诱导了等同或更优的细胞免疫(图6)。
另外，针对布里斯班肽文库对T细胞表位作图。Mosaic疫苗产生了最高水平的识别肽，接着是CoUS疫苗免疫原，分别为6种和4种肽(图7)。分别将其与针对3种和1种肽的野生型应答进行比较。Mosaic、CoUS、PR8和pd09HA疫苗分别诱导针对5、4、3和1表位的T细胞应答(图7)。这些经验数据显示，在所有疫苗策略中，Mosaic疫苗策略确实选择了最大的T细胞覆盖度和期望的B细胞覆盖度。
为了确定本文所述的独特免疫原设计是否比野生型免疫原更好地诱导抗体，用等效感染单位的表达Ad-HA的病毒感染细胞。收获蛋白质并且将其通过蛋白质印迹进行筛选。观察到与野生型TX98 HA蛋白相比，通过不同的A/猪/印第安纳州/0392/2011病毒免疫的山羊的血清更容易识别所有新型中心化和优化的疫苗免疫原。由于与野生型TX/98HA相比更容易检测到新型疫苗免疫原，因此这可能表明这些HA疫苗在呈递B细胞表位和刺激体液免疫方面可能更好(图8)。
野生型、COT、CoUS、Mosaic和Epigraph基因的合成和克隆。以计算方式设计并且分析人HA共有序列COT、CoUS、Mosaic和Epigraph免疫原。由于靶标疫苗是人的，因此对基因进行针对人的密码子优化。当前的2018-19免疫实施咨询委员会(Advisory Committee onImmunization Practices，ACIP)建议将H1和H3二者的野生型代表性HA基因包括在内。用作H1比较物的野生型毒株是A/密歇根州/45/2015(H1N1)pdm09类。用作H3比较物的野生型毒株是A/新加坡/INFIMH-16-0019/2016(H3N2)类病毒。所有密码子优化的HA基因均被合成并且克隆至pcDNA3.1中，以用于T7体外转录(IVT)。所有疫苗基因都是由GenscriptCorporation合成构建的。
mRNA和脂质纳米颗粒(LNP)疫苗的产生和质量控制。当比较疫苗免疫原时，重要的是疫苗的质量是等同的。因此，通过以下方法对所有mRNA-LNP疫苗进行质量控制：通过电泳和密度测定法分析来分析mRNA的质量以及通过蛋白质印迹测定来分析定量蛋白质表达。所有mRNA均使用HiScribeTMT7 ARCA mRNA试剂盒(带尾)来产生。
简而言之，使用HindIII或XmaI将含有野生型和优化疫苗免疫原的pcDNA3.1质粒线性化，进行纯化并且将其用作模板。使用T7 RNA聚合酶从模板转录RNA，并且将5mCTP和假UTP并入以减少先天免疫信号传导。使用ARCA加帽酶对RNA进行加帽，并且使用大肠杆菌聚(A)聚合酶对其进行聚腺苷酸化。mRNA质量通过电泳来确定，通过UV光谱法定量，等分并且在-80℃下储存。使用微流体混合将脂质DSPC、胆固醇(Sigma-Aldrich)、DMAP-BLP、PEG-DMG2K和PEG-DSG2K(NOF America Corporation)以50:10:39.5:0.5的摩尔比溶解在乙醇中。将mRNA与脂质:乙醇溶液以3:1比率在50mM柠檬酸盐(pH-4.0)中混合。使用100K MWCO透析盒相对于PBS(pH-7.4)将mRNA-LNP透析过夜，并且将其使用Amicon超离心过滤器浓缩。最终的mRNA-LNP产物在4℃下储存。通过电子显微镜检查确定mRNA-LNP的大小和质量。
对代表性疫苗候选物进行剂量滴定以确定疫苗剂量。先前的研究已显示，少至2μg的mRNA-LNP可以保护抵抗致死寨卡病毒激发。然而，由于我们正在使用高致死流感激发模型，因此我们可能需要使用更高剂量的mRNA-LNP疫苗剂量进行免疫或使用初免加强策略以达到保护水平。我们通过以下方法来证实这种保护作用：用一系列剂量的疫苗剂量(2、10和20μg/小鼠)免疫5只小鼠的组并且在疫苗接种后5周用同源A/PR/8/34病毒进行激发。如果在最高剂量下未观察到完全保护作用，则将第三组以20μg/小鼠进行初免并且加强。加强后3周，用致死流感激发对小鼠进行激发。如果初免/加强免疫未能提供完全保护作用，则探索初免/加强/加强疫苗策略，以确定能够提供完全保护作用的最佳疫苗剂量。
使用氯胺酮/赛拉嗪麻醉动物以进行所有疫苗接种和病毒激发。肌内免疫由在PBS中稀释的50μl疫苗组成，并且在两次25μl注射中使用27号针头注射到两个股四头肌中。最初，所有免疫都是通过肌内注射途径进行的。所有最初的研究都是以单剂量疫苗药剂的形式进行的。用10-100MLD50小鼠适应性流感病毒对小鼠进行激发，并且监测疾病和死亡。
免疫相关因素如HI滴度和T细胞ELISpot测定。分析针对至少5-H1和5-H3流感毒株的免疫相关因素。该数据允许我们推断出由各种疫苗诱导的抗流感免疫的真正广度和强度。分析细胞和体液免疫两者，因为两者在保护和清除中起着关键作用。根据制造商的建议，将所有候选疫苗应答与商业灭活FluSure疫苗进行比较。
血凝抑制(HI)测定。疫苗效力的主要决定因素一直是HI测定。由于不能在小鼠中评价所有流感病毒，因此HI测定允许全面评价由免疫原诱导的交叉保护性免疫应答。因此，使用HI测定来测定诱导的交叉反应性抗体。
中和测定。为了测定血清对同源和异源血清的功能性中和，进行中和测定。
细胞免疫(ELISpot)。为了确定由各种疫苗免疫原诱导的细胞免疫的广度和量级，如先前所述(REF 60)我们通过IFN-γELISpot进行了表位作图并对干扰素分泌细胞的绝对数量进行定量，并显示在图6和图7中。
用于确定最有效的疫苗策略的激发研究。确定疫苗效力的最有效方法是实际的流感激发研究。对H1和H3疫苗单独地进行测试，以消除疫苗免疫原之间的任何潜在干扰。疫苗组由单个野生型、COT、CoUS或Mosaic HA免疫原组成。将epigraph免疫原作为混合物递送。与单独HA疫苗相比，HA-Epi疫苗由相同的最终剂量的mRNA-LNP组成。由于Epigraph疫苗由3种epigraph免疫原的混合物组成，因此每种Epi mRNA以最终剂量的1/3包含在疫苗中。例如，如果疫苗剂量为10μg/小鼠，则Epigraph疫苗由3.33μg的每种Epi mRNA-LNP疫苗组成。对于每种激发病毒的5只对照小鼠的组被包括在内。将所有疫苗与以小鼠等效剂量接受商业四价FluZone的组进行比较。
确定来自H1和H3疫苗激发研究的疫苗候选物是否可以合并为针对两种亚型的一种通用疫苗。理想的疫苗需要尽可能少的免疫，并保护免于所有病毒变体。因此，我们确定了是否可以将由针对每种流感亚型的最有效疫苗接种方法构成的单次免疫合并以制备诱导针对H1N1和H3N2流感病毒的免疫的通用疫苗。这假设了一种疫苗免疫原方法对于H1和H3流感亚型二者更优。然而，一种方法很可能比另一种方法更有效，因此可以测试由两种不同疫苗免疫原构成的多价疫苗。例如，epigraph方法可能针对H1N1流感更有效，而Mosaic方法可能对H3N2流感更有效。因此，测试了Ad-H1-Epi+Ad-H3-Mos多价疫苗对所有六种流感病毒的效力。对于每种激发病毒的5只对照小鼠的组被包括在内。
由于先前的疫苗接种，在存在先前免疫的情况下进行免疫。这些实验模拟了由季节性灭活疫苗而不是自然感染引发的免疫系统。这些情况更准确地类似于接种疫苗的儿童的免疫系统。用季节性灭活FluZone疫苗免疫五只小鼠的组。六个月后，用野生型或通用多价优化疫苗对小鼠进行再免疫。用一组流感病毒对小鼠进行激发以确定交叉保护性免疫的程度。重复所有小鼠研究。
由于自然感染，在存在先前免疫的情况下进行免疫。为了模拟自然感染产生的免疫力，用低剂量的野生型流感病毒感染小鼠。这些情况更准确地类似于成年人的免疫系统。以与先前描述的对于由于疫苗接种而具有先前免疫的动物相同的方式，在该免疫库的背景下测试了毒株特异性野生型和通用疫苗诱导交叉保护性免疫的能力。重复所有小鼠研究。
流感动物模型。雪貂模型非常类似于人的流感感染。雪貂具有相似的临床症状，并且对流感感染同样敏感。雪貂模型被认为是评价流感致死性、毒力、感染性和疫苗效力的最佳动物模型(Herlocher等人,2001,J.Infect.Dis.,184:542-6；Maassab等人,1982,J.Infect.Dis.,146:780-90；Sweet和Smith,1980,Microbiol.Rev.,44:303-30)。同样，由于交叉保护性免疫应答的初步评价是使用BALB/c小鼠进行的，因此在远缘杂交遗传背景下确定免疫应答很重要。因此，雪貂模型用于最有效的疫苗的最终评价。重复所有雪貂研究。
雪貂用于最有前途的广泛保护性疫苗的最终分析。用野生型、通用和FluZone疫苗免疫五只雪貂的组。免疫后三周获得血清和鼻洗液。使用HI和微中和测定对大量流感病毒进行了测定，以对交叉保护性免疫应答进行更广泛的分析。用每种亚型的至少四种代表性病毒对经免疫的雪貂进行激发。每天监测雪貂的温度和脱水情况。雪貂发热很少达到危险水平，并且有迹象表明减少发热实际上增加病毒血症。如果动物脱水，则每天通过皮下注射10ml无菌盐水进行治疗。将垂死的动物处死。使用如通过体重减轻、临床得分和鼻病毒脱落测量的疾病来评价每种疫苗的保护性应答。
实施例3-代表性序列比较
如表1所示，将本文所述的选择的疫苗多肽的序列彼此相互比较。
表1.选择的流感H1 HA蛋白序列的比较
Figure GDA0003214791590000321
实施例4-用于检测疫苗免疫原的蛋白质印迹
用500个病毒颗粒/细胞感染293细胞，并在48小时后收获。用来自A/PR/8/34毒株的抗HA0抗体(NR-3148)和抗HA1抗体作为一抗来探测蛋白质印迹。对于所有三种重组腺病毒均观察到HA0(85kDa)和HA1(65kDa)条带，并且其以相似水平表达(图24C)。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后收获血清(n＝8)。用RDE处理血清，并使用HI滴度从1:10稀释度开始测量抗体。通过血凝抑制测定来测量流感毒株A/CA/07/09(图25A)、A/NC/20/99(图25B)、A/PR/8/34(图25C)、A/WS/33(图25D)和A/FM/1/47(图25E)的抗体。结果显示为阻止血凝的最高稀释度的倒数，并且阴性结果显示为检测限的一半。
用10e10病毒颗粒、150ng Fluzone HA或PBS免疫雌性Balb/c小鼠，并在3周后用相同剂量加强(n＝5)。加强后10天收获血清，并将其用RDE处理，从而使用HI滴度从1:10稀释度开始测量抗体。通过血凝抑制测定来测量流感毒株A/CA/07/09(图26A)、A/NC/20/99(图26B)、A/PR/8/34(图26C)、A/WS/33(图26D)和A/FM/1/47(图26E)的抗体。结果显示为阻止血凝的最高稀释度的倒数，并且阴性结果显示为检测限的一半。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后收获血清(n＝8)。将血清稀释1:100并使用ELISA测定测量抗体。用来自流感毒株A/CA/04/09(图27A)、A/PR/8/34(图27B)、A/NC/20/99(图27C)和A/布里斯班/59/07(图27D)的重组HA蛋白以200ng/孔包被平板，并测量OD450处的吸光度。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后收获脾细胞(n＝8)。使用跨越A/加利福尼亚州/07/09(图28A)、A/波多黎各/8/34(图28B)、A/新喀里多尼亚/20/99(图28C)或A/布里斯班/59/07(图28D)毒株的整个HA基因的肽阵列，通过IFNγELISpot测定测量总T细胞应答，并将其表示为每百万个脾细胞中斑点形成细胞(SFC)的平均数量。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后收获脾细胞(n＝8)。使用跨越整个HA基因的重叠肽文库对T细胞表位作图。大于每百万个脾细胞中50个斑点形成细胞(SFC)的应答被认为是阳性的，并且关于HA基因定位进行显示(图29A)。对A/布里斯班/59/07(图29B)、A/新喀里多尼亚/20/99(图29C)和A/加利福尼亚州/07/09(图29D)的阳性肽进行定量。肽毒株(灰色)、mosaic(绿色)、A/PR/8/34(深蓝色)和A/TX/05/09pdm09(浅蓝色)HA序列的比对直接显示在阳性肽下面，从而指示可能的表位定位。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后用100MLD50对其进行激发(n＝5)。激发后14天监测小鼠，并且如果记录25％体重减轻，则将其人道地处死。监测单独小鼠的体重减轻(图30A、图30B)、平均体重减轻(图30C、图30D)和存活率百分比(图30E、图30F)。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后用100小鼠致死剂量(MLD)50对其进行激发(n＝5)。激发后14天监测小鼠，并且如果记录25％体重减轻，则将其人道地处死。测定单独小鼠的体重减轻(图31A、图31B)、平均体重减轻(图31C、图31D)和存活率百分比(图31E、图31F)。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后用100MLD50对其进行激发(n＝5)。激发后14天监测小鼠，并且如果记录25％体重减轻，则将其人道地处死。测定单独小鼠的体重减轻(图32A、图32B)、平均体重减轻(图32C、图32D)和存活率百分比(图32E、图32F)。
用重组Ad5-H1-Mosaic-HA、Ad5-A/PR/8/34-HA、Ad5-A/TX/05/09-HA(pdm09)的10e8或10e10病毒颗粒、150ng Fluzone HA或者PBS免疫雌性Balb/c小鼠，并在3周后用100MLD50对其进行激发(n＝5)。激发后14天监测小鼠，并且如果记录25％体重减轻，则将其人道地处死。测定单独小鼠的体重减轻(图33A、图33B)、平均体重减轻(图33C、图33D)和存活率百分比(图33E、图33F)。
实施例5-猪流感和通用疫苗血凝素的系统发育树
从流感研究数据库下载所有1,561种独特猪H3流感病毒HA蛋白(不包括实验室毒株和重复毒株)。通过HIV数据库工具套件可得到的Mosaic疫苗设计和Epigraph程序分别用于创建Mosaic和Epigraph候选疫苗蛋白。然后使用ClustalW将Mosaic和三种Epigraph免疫原与原始序列群体比对，并构建了Jukes-Cantor邻接树(图34A)。合成这些通用免疫原并将其克隆至复制缺陷型腺病毒5载体中。使用野生型比较物HA基因(A/猪/得克萨斯州/4199-2/1998[TX98])作为对照，用等效的感染单位感染293细胞，并且通过用多克隆山羊抗A/猪/印第安纳州/0392/2011血清探测蛋白质印迹来检测血凝素蛋白表达(图34B)。
将5至10只小鼠的组用表达Mosaic、Epigraph或野生型TX98 HA免疫原的Ad5载体疫苗的1010病毒颗粒(vp)免疫(图35A)。另外，与3周大的仔猪相比，以相当于10倍剂量的剂量用商业FluSure免疫小鼠。单次免疫后，在第3周进行颌下出血以评价抗体。然后对小鼠进行加强，并在加强后14天收获血清(图35A)。
选择代表性相异H3猪流感病毒用于抗体发育的分析(图35B)，并按照世界卫生组织标准方案的描述进行血凝抑制(HI)(图35C)。虚线表示标准滴度或40，其被认为提供针对激发的保护作用。
如先前在图35中所述向Balb/c小鼠(n＝5)接种表达Mosaic、Epigraph和野生型TX98 HA免疫原的Ad载体疫苗。使用由10个氨基酸重叠的17-mer的重叠肽库来测定针对四种代表性相异swH3病毒由疫苗免疫原诱导的T细胞表位。通过ELISpot测定测量诱导干扰素-γ的T细胞。如果斑点形成细胞(SFC)/百万大于50，则认为肽为阳性(图36A)。还测量了对阳性肽的单独应答(图36B)和总T细胞应答(图36C)。
如先前对于图35所述，对Balb/c小鼠(n＝5)进行免疫。将来自接种疫苗的小鼠的血清与代表性人H3N2病毒分离物一起孵育，并测定几何平均HI滴度±标准误差(图37A)。使用重叠的肽库和IFN-γELISpot测定评价了脾细胞针对四种代表性人流感毒株的交叉反应性T细胞免疫应答。如果斑点形成细胞(SFC)/百万大于50，则认为肽为阳性(图37B)。还测量了对阳性肽的单独应答(图37C)和总T细胞应答(图37D)。
向Balb/c小鼠(n＝5)接种10e10 vp单剂量的表达Mosaic、Epigraph或TX98免疫原的Ad5载体疫苗，并与接种FluSure的动物或假接种的动物进行比较。三周后，用10e5TCID50的曼尼托巴省/2005(图38A)或10e3.5 TCID50的科罗拉多州/1977(图38B)鼻内感染小鼠，并监测体重减轻。另外，如先前所述，向一组小鼠(n＝5)接种疫苗并使其感染，但在感染后第3天处死，以通过qPCR分析肺病毒滴度(右侧)。
应当理解的是，虽然在本文中已经结合多个不同方面描述了所述方法和物质组合物，但是各个方面的前述描述旨在说明而不是限制所述方法和物质组合物的范围。其他方面、优点和修改在以下权利要求书的范围内。
公开了如下方法和组合物，所述方法和组合物可以用于所公开的方法和组合物的产物、可以与所公开的方法和组合物的产物结合使用、可以用于制备所公开的方法和组合物的产物；或者公开了所公开的方法和组合物的产物。本文公开了这些和其他材料，并且应理解的是，公开了这些方法和组合物的组合、子集、相互作用、组等。也就是说，虽然可能未明确地公开对这些组合物和方法的每一种不同的单独和集体组合和排列的特别提及，但是在本文中特别地考虑并描述了每一种。例如，如果公开并讨论了特定的物质组合物或特定方法，并且讨论了多个组合物或方法，则除非另有明确指示，否则所述组合物和所述方法的每个和每种组合和排列都是特别考虑的。同样地，这些的任何子集或组合也是特别考虑并公开的。
序列表
<110> 内布拉斯加大学董事会
<120> 制备和使用通用集中型流感疫苗基因的方法
<130> 24742-0115WO1
<150> 62/734,791
<151> 2018-09-21
<160> 122
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Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Ala Thr Ala Asn
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Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
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Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
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Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly
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Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
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Val Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
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Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Ala Asp Gln Gln Ser Leu Tyr
195 200 205
Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys
210 215 220
Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln Glu
225 230 235 240
Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile
245 250 255
Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Ala
260 265 270
Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val
275 280 285
His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn Thr
290 295 300
Ser Leu Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly
305 310 315 320
Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly
325 330 335
Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile
340 345 350
Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr
355 360 365
Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu
370 375 380
Lys Ser Thr Gln Asn Ala Ile Asp Ile Thr Asn Lys Val Asn Ser Val
385 390 395 400
Ile Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn
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His Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly
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Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu
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Asn Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr
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Glu Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn
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Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser
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Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys
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Leu Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile
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Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu
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Val Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser
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Ser Val Glu Tyr Ser Leu Gln Cys Arg Ile Cys Ile
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Met Lys Val Lys Leu Leu Val Leu Leu Cys Thr Phe Thr Ala Thr Tyr
1 5 10 15
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
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Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile
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Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Pro Asn Pro Glu Asn Gly Thr Cys Tyr Pro Gly Phe Ala
100 105 110
Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu
115 120 125
Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr Val
130 135 140
Thr Lys Gly Val Ser Ala Ser Cys Ser His Asn Gly Ser Ser Phe Tyr
145 150 155 160
Arg Asn Leu Leu Trp Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn Leu
165 170 175
Ser Lys Ser Tyr Ala Asn Asn Lys Glu Lys Glu Val Leu Val Leu Trp
180 185 190
Gly Val His His Pro Pro Asn Ile Gly Asp Gln Leu Tyr His Thr Glu
195 200 205
Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg Phe Thr Pro
210 215 220
Glu Ile Ala Lys Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Ile Asn
225 230 235 240
Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile Ile Phe Glu Ala
245 250 255
Asn Gly Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala Leu Ser Arg Gly
260 265 270
Phe Gly Ser Gly Ile Ile Ser Asn Ala Pro Met Asp Glu Cys Asp Ala
275 280 285
Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser Ser Leu Pro Phe Gln
290 295 300
Asn Val His Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Arg Ser
305 310 315 320
Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln
325 330 335
Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp
340 345 350
Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln
355 360 365
Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asn
370 375 380
Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln
385 390 395 400
Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu Glu Arg Arg Met Glu
405 410 415
Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr
420 425 430
Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe
435 440 445
His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Lys Ser Gln Leu
450 455 460
Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His
465 470 475 480
Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp
485 490 495
Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg Glu Lys Ile Asp
500 505 510
Gly Val Lys Leu Glu Ser Met Gly Val Tyr Gln Ile Leu Ala Ile Tyr
515 520 525
Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser Leu Gly Ala Ile
530 535 540
Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile
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Met Lys Val Lys Leu Leu Val Leu Leu Cys Thr Phe Thr Ala Thr Tyr
1 5 10 15
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile
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Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly
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Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile
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Val Glu Lys Pro Asn Pro Glu Asn Gly Thr Cys Tyr Pro Gly His Phe
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Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Leu
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr
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Val Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Glu Ser Ser Phe
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Tyr Arg Asn Leu Leu Trp Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn
165 170 175
Leu Ser Lys Ser Tyr Ala Asn Asn Lys Glu Lys Glu Val Leu Val Leu
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Trp Gly Val His His Pro Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr
195 200 205
His Thr Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg
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Lys Phe Thr Pro Glu Ile Ala Lys Arg Pro Lys Val Arg Asp Gln Glu
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Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile
245 250 255
Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala
260 265 270
Leu Ser Arg Gly Phe Gly Ser Gly Ile Ile Asn Ser Asn Ala Pro Met
275 280 285
Asp Lys Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser
290 295 300
Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro
305 310 315 320
Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn
325 330 335
Ile Pro Ser Val Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe
340 345 350
Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His
355 360 365
His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr
370 375 380
Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu
385 390 395 400
Lys Met Asn Ile Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu
405 410 415
Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile
420 425 430
Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu
435 440 445
Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys
450 455 460
Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Val Gly Asn Gly Cys
465 470 475 480
Phe Glu Phe Tyr His Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys
485 490 495
Asn Glu Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn
500 505 510
Arg Glu Lys Ile Asp Gly Val Lys Leu Asp Ser Met Gly Val Tyr Gln
515 520 525
Ile Leu Thr Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val
530 535 540
Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln
545 550 555 560
Cys Lys Ile Cys Ile
565
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Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Pro Asn Lys Gly Val Thr Thr Ala Cys Pro His Ala Gly Ala Lys Ser
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Val Glu Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Ile Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Thr Tyr Val Phe Val Gly Thr Ser Lys Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Ile Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Thr Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Ser
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe His Asn Ile His Pro Ile Thr Ile Gly Glu Cys
305 310 315 320
Pro Lys Tyr Val Arg Ser Thr Lys Leu Arg Met Val Thr Gly Leu Arg
325 330 335
Asn Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Ala Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys
405 410 415
Leu Glu Lys Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Thr Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asn Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val
530 535 540
Val Ser Leu Gly Ala Val Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Arg Cys Arg Ile Cys Ile
565
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<212> PRT
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Met Glu Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Ala Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Leu Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Arg Leu Lys Gly Ile
50 55 60
Ala Pro Leu Gln Leu Gly Lys Cys Ser Ile Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Thr Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Met Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Gly
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Arg Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys
305 310 315 320
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg
325 330 335
Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Leu Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Met Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Gly Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Asp Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His
405 410 415
Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Ile Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Val Leu Glu Asn
435 440 445
Glu Arg Thr Leu Glu Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asn Asn Glu Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Ala Ala Ser Ser Leu Val Leu Val
530 535 540
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Ile Cys Ile
565
<210> 6
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 6
Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Ala Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys
305 310 315 320
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg
325 330 335
Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His
405 410 415
Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val
530 535 540
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Ile Cys Ile
565
<210> 7
<211> 563
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 7
Met Lys Val Lys Leu Leu Val Leu Leu Cys Thr Phe Thr Ala Thr Tyr
1 5 10 15
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile
50 55 60
Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile
85 90 95
Val Glu Lys Pro Asn Pro Glu Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Leu
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr
130 135 140
Val Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Glu Ser Ser Phe
145 150 155 160
Tyr Arg Asn Leu Leu Trp Leu Thr Gly Lys Asn Gly Leu Tyr Pro Asn
165 170 175
Leu Ser Lys Ser Tyr Ala Asn Asn Lys Glu Lys Glu Val Leu Val Leu
180 185 190
Trp Gly Val His His Pro Pro Asn Ile Gly Asp Gln Arg Ala Leu Tyr
195 200 205
His Thr Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg
210 215 220
Lys Phe Thr Pro Glu Ile Ala Lys Arg Pro Lys Val Arg Asp Gln Glu
225 230 235 240
Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile
245 250 255
Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Arg Tyr Ala Phe Ala
260 265 270
Leu Ser Arg Gly Phe Gly Ser Gly Ile Ile Asn Ser Asn Ala Pro Met
275 280 285
Asp Lys Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser
290 295 300
Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro
305 310 315 320
Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn
325 330 335
Ile Pro Ser Ile Gln Ser Arg Gly Leu Leu Gly Ala Ile Ala Gly Phe
340 345 350
Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His
355 360 365
His Gln Asn Ala Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr
370 375 380
Gln Asn Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile Glu
385 390 395 400
Lys Met Asn Ile Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu
405 410 415
Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Ile
420 425 430
Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn Glu
435 440 445
Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys
450 455 460
Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Val Gly Asn Gly Cys
465 470 475 480
Phe Glu Phe Tyr His Lys Cys Asn Asp Glu Cys Met Glu Ser Val Lys
485 490 495
Asn Gly Ile Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn
500 505 510
Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr Gln
515 520 525
Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val
530 535 540
Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Gly His Ser Cys Arg
545 550 555 560
Ile Cys Ile
<210> 8
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 8
Met Glu Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Val Leu Glu Arg Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Arg Leu Lys Gly Ile
50 55 60
Ala Pro Leu Gln Leu Gly Lys Cys Asn Ile Ala Gly Trp Leu Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Phe Ser Lys Glu Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Pro Asn Pro Glu Asn Gly Thr Cys Tyr Pro Gly His Phe
100 105 110
Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Asn Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Met Phe Pro Lys Thr Ser Ser Trp Pro Asp His Asp
130 135 140
Ser Asn Lys Gly Val Thr Thr Ala Cys Pro His Ala Gly Ala Lys Gly
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Thr Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Ile Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Thr Tyr Val Phe Val Gly Thr Ser Lys Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Arg Arg Met Asn Tyr Tyr Trp Thr Leu Ile Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Ala Asn Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Thr Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Ser
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe His Asn Ile His Pro Ile Thr Ile Gly Glu Cys
305 310 315 320
Pro Lys Tyr Val Arg Ser Thr Lys Leu Arg Met Val Thr Gly Leu Arg
325 330 335
Asn Val Pro Ser Val Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Met Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Gly Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Asp Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys
405 410 415
Leu Glu Lys Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Ile Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Tyr His Asp Ser Lys Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Thr Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asn Asn Glu Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asp Tyr Pro Arg Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Val Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Ala Ala Ser Ser Leu Val Leu Val
530 535 540
Val Ser Leu Gly Glu Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Arg Cys Arg Ile Cys Ile
565
<210> 9
<211> 558
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 9
Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Thr Ala Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Val Pro Glu Trp Ser Tyr Ile Met Glu Lys
85 90 95
Glu Asn Pro Arg Gly Leu Cys Tyr Pro Gly Ser Phe Asn Asp Tyr Glu
100 105 110
Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys Val Lys
115 120 125
Ile Leu Pro Lys Asp Arg Trp Thr Gln His Thr Thr Thr Gly Gly Ser
130 135 140
Arg Ala Cys Ala Val Ser Gly Asn Pro Ser Phe Phe Arg Asn Met Val
145 150 155 160
Trp Leu Thr Lys Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly Ser Tyr
165 170 175
Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val His His
180 185 190
Pro Asn Asp Glu Thr Glu Gln Arg Thr Leu Tyr Gln Asn Val Gly Thr
195 200 205
Tyr Val Ser Val Thr Ser Thr Leu Asn Lys Arg Ser Thr Pro Glu Ile
210 215 220
Ala Arg Pro Lys Val Asn Gly Leu Gly Ser Arg Met Glu Phe Ser Trp
225 230 235 240
Thr Leu Leu Asp Met Trp Asp Thr Ile Asn Phe Glu Ser Thr Gly Asn
245 250 255
Leu Ile Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys Arg Gly Ser Ser
260 265 270
Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys Glu Thr Lys Cys
275 280 285
Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro Phe His Asn Val
290 295 300
His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Glu Lys
305 310 315 320
Leu Val Leu Ala Thr Gly Leu Arg Asn Val Pro Gln Ile Glu Ser Arg
325 330 335
Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly
340 345 350
Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Asp Gln Gly Ser
355 360 365
Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala Phe Asp Gly Ile
370 375 380
Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Glu
385 390 395 400
Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg Leu Glu Asn Leu
405 410 415
Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala
420 425 430
Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe His Asp
435 440 445
Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Met Gln Leu Arg Asp
450 455 460
Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys
465 470 475 480
Asp Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro
485 490 495
Lys Tyr Glu Glu Glu Ser Lys Leu Asn Arg Asn Glu Ile Lys Gly Val
500 505 510
Lys Leu Ser Ser Met Gly Val Tyr Gln Ile Leu Ala Ile Tyr Ala Thr
515 520 525
Val Ala Gly Ser Leu Ser Leu Ala Ile Met Met Ala Gly Ile Ser Phe
530 535 540
Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile
545 550 555
<210> 10
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 10
Met Val Ile Ile Tyr Leu Ile Leu Leu Phe Thr Ala Val Arg Gly Asp
1 5 10 15
Lys Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Met Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asn Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Arg Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Arg Asp Gly Leu Cys Tyr Pro Gly Ser Phe Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Phe Leu Ser Ser Val Lys His Phe Glu Lys
115 120 125
Val Lys Ile Leu Pro Lys Asp Arg Trp Thr Gln His Thr Thr Thr Gly
130 135 140
Gly Ser Arg Ala Cys Ala Val Ser Gly Asn Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Lys Lys Gly Ser Asn Tyr Pro Val Ala Gln Gly
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Lys Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Asn Asp Glu Thr Glu Gln Arg Thr Leu Tyr Gln Asn Val
195 200 205
Gly Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Lys Arg Ser Thr
210 215 220
Pro Asp Ile Ala Thr Arg Pro Lys Val Asn Gly Leu Gly Gly Arg Met
225 230 235 240
Glu Phe Ser Trp Thr Leu Leu Asp Met Trp Asp Thr Ile Asn Phe Glu
245 250 255
Ser Thr Gly Asn Leu Ile Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Arg Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Ala Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Arg Ser Glu Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His Ser Asn
355 360 365
Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Arg Ala
370 375 380
Phe Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn
385 390 395 400
Thr Gln Phe Glu Ser Val Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg
405 410 415
Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu
435 440 445
Gly Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asn Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asp Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asn Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr
485 490 495
Tyr Tyr Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Asn Arg Asn Glu
500 505 510
Ile Lys Gly Val Lys Leu Ser Ser Met Gly Ile Tyr Gln Ile Leu Ala
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Met Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Phe Arg Ile
545 550 555 560
Cys Ile
<210> 11
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 11
Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Ala Thr Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Thr Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu
35 40 45
Glu Lys Ala His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Lys Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Lys Tyr Ser Leu Cys Tyr Pro Gly Ser Leu Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys
115 120 125
Val Arg Ile Leu Pro Lys Asp Gly Trp Thr Gln His Thr Thr Thr Gly
130 135 140
Gly Ser Lys Ala Cys Ala Val Ser Gly Lys Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Glu Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Ile Asp Glu Ala Glu Gln Arg Ala Leu Tyr Gln Glu Val
195 200 205
Gly Thr Tyr Val Ser Ala Ser Thr Ser Thr Leu Asn Lys Arg Ser Ile
210 215 220
Pro Glu Ile Ala Ala Arg Pro Lys Val Asn Gly Leu Gly Ser Arg Met
225 230 235 240
Glu Phe Phe Trp Thr Leu Leu Asp Val Trp Asp Thr Ile Asn Phe Glu
245 250 255
Ser Thr Gly Asn Leu Val Val Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Gly Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Arg Tyr Val
305 310 315 320
Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ile Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn
355 360 365
Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala
370 375 380
Phe Asp Gly Ile Thr Asn Lys Glu Asn Ser Val Ile Glu Lys Met Asn
385 390 395 400
Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Asn Asn Leu Glu Lys Arg
405 410 415
Leu Glu Asn Leu Asn Lys Arg Met Glu Asp Gly Phe Leu Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu
435 440 445
Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asp Asn Glu Cys Met Asp Ser Val Lys Asn Gly Thr
485 490 495
Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Ala Lys Arg Asn Glu
500 505 510
Ile Lys Gly Val Lys Leu Ser Ser Met Gly Val Tyr Gln Ile Leu Ala
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Val Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile
545 550 555 560
Cys Ile
<210> 12
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 12
Met Ala Ile Ile Tyr Phe Ile Leu Leu Phe Thr Ala Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asn Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Arg Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Thr Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Arg Tyr Ser Leu Cys Tyr Pro Gly Ser Phe Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Leu Glu Lys
115 120 125
Val Lys Ile Leu Pro Lys Asp Gly Trp Thr Gln His Glu Thr Asp Gly
130 135 140
Gly Ser Lys Ala Cys Ala Val Ser Gly Glu Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Lys Lys Gly Pro Asn Tyr Pro Val Ala Lys Arg
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Asn Asp Glu Ala Glu Gln Arg Ala Leu Tyr Gln Lys Val
195 200 205
Gly Thr Tyr Val Ser Ala Ser Thr Ser Thr Leu Asn Lys Arg Ser Thr
210 215 220
Pro Glu Ile Ala Ala Arg Pro Glu Val Asn Gly Leu Gly Ser Arg Met
225 230 235 240
Glu Phe Ser Trp Thr Leu Leu Asp Val Trp Asp Thr Ile Ser Phe Glu
245 250 255
Ser Thr Gly Asn Leu Val Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Ser Ser Gly Ile Ile Lys Thr Glu Gly Thr Leu Gly Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Pro Arg Asn Val Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Lys
355 360 365
Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala
370 375 380
Phe Asp Arg Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Ile Asn
385 390 395 400
Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Lys Arg
405 410 415
Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Ile Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Ile Leu
435 440 445
Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asp Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr
485 490 495
Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Lys Arg Asn Glu
500 505 510
Ile Lys Gly Val Glu Leu Ser Ser Met Gly Val Tyr Gln Ile Leu Val
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Ile Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Val
545 550 555 560
Cys Ile
<210> 13
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 13
Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Thr Ala Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asp Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Arg Asp Gly Leu Cys Tyr Pro Gly Ser Phe Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys
115 120 125
Val Lys Ile Leu Pro Lys Asp Arg Trp Thr Gln His Thr Thr Thr Gly
130 135 140
Gly Ser Arg Ala Cys Ala Val Ser Gly Asn Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Lys Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Asn Asp Glu Thr Glu Gln Arg Thr Leu Tyr Gln Asn Val
195 200 205
Gly Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Lys Arg Ser Thr
210 215 220
Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly Leu Gly Ser Arg Met
225 230 235 240
Glu Phe Ser Trp Thr Leu Leu Asp Met Trp Asp Thr Ile Asn Phe Glu
245 250 255
Ser Thr Gly Asn Leu Ile Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Val Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn
355 360 365
Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala
370 375 380
Phe Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn
385 390 395 400
Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Arg Arg
405 410 415
Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu
435 440 445
Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asp Asp Glu Cys Met Asn Ser Val Lys Asn Gly Thr
485 490 495
Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Asn Arg Asn Glu
500 505 510
Ile Lys Gly Val Lys Leu Ser Ser Met Gly Val Tyr Gln Ile Leu Ala
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Met Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile
545 550 555 560
Cys Ile
<210> 14
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 14
Met Ala Ile Ile Tyr Leu Ile Leu Leu Phe Ala Thr Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Thr Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asn Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Arg Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Arg Tyr Ser Leu Cys Tyr Pro Gly Ser Leu Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys
115 120 125
Val Arg Ile Leu Pro Lys Asp Gly Trp Thr Gln His Thr Thr Thr Gly
130 135 140
Gly Ser Lys Ala Cys Ala Val Ser Gly Lys Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Lys Lys Gly Pro Asn Tyr Pro Val Ala Lys Arg
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Asn Asp Glu Ala Glu Gln Arg Ala Leu Tyr Gln Lys Val
195 200 205
Gly Thr Tyr Val Ser Ala Ser Thr Ser Thr Leu Asn Lys Arg Ser Thr
210 215 220
Pro Asp Ile Ala Thr Arg Pro Lys Val Asn Gly Gln Gly Gly Arg Met
225 230 235 240
Glu Phe Ser Trp Thr Leu Leu Asp Val Trp Asp Thr Ile Asn Phe Glu
245 250 255
Ser Thr Gly Asn Leu Val Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Gly Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Pro Arg Asn Val Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn
355 360 365
Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala
370 375 380
Phe Asp Arg Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn
385 390 395 400
Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Asn Asn Leu Glu Lys Arg
405 410 415
Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu
435 440 445
Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asp Asn Glu Cys Met Asp Ser Val Lys Asn Gly Thr
485 490 495
Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Lys Arg Asn Glu
500 505 510
Ile Lys Gly Val Lys Leu Ser Ser Met Gly Ile Tyr Gln Ile Leu Ala
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Ile Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile
545 550 555 560
Cys Ile
<210> 15
<211> 562
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 15
Met Ala Ile Ile Tyr Phe Ile Leu Leu Phe Thr Ala Val Arg Gly Asp
1 5 10 15
Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys Val Asp
20 25 30
Thr Ile Leu Glu Arg Asn Val Thr Val Thr His Ala Lys Asn Ile Leu
35 40 45
Glu Lys Thr His Asn Gly Lys Leu Cys Lys Leu Asn Gly Ile Pro Pro
50 55 60
Leu Glu Leu Gly Asp Cys Ser Ile Ala Gly Trp Leu Leu Gly Asn Pro
65 70 75 80
Glu Cys Asp Arg Leu Leu Ser Val Pro Glu Trp Ser Tyr Ile Met Glu
85 90 95
Lys Glu Asn Pro Lys Tyr Ser Leu Cys Tyr Pro Gly Ser Phe Asn Asp
100 105 110
Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Val Lys His Phe Glu Lys
115 120 125
Val Lys Ile Leu Pro Lys Asp Gly Trp Thr Gln His Glu Thr Asp Gly
130 135 140
Gly Ser Lys Ala Cys Ala Val Ser Gly Glu Pro Ser Phe Phe Arg Asn
145 150 155 160
Met Val Trp Leu Thr Glu Lys Gly Ser Asn Tyr Pro Val Ala Lys Gly
165 170 175
Ser Tyr Asn Asn Thr Ser Gly Glu Gln Met Leu Ile Ile Trp Gly Val
180 185 190
His His Pro Ile Asp Glu Ala Glu Gln Arg Ala Leu Tyr Gln Glu Val
195 200 205
Gly Thr Tyr Val Ser Ala Ser Thr Ser Thr Leu Asn Lys Arg Ser Ile
210 215 220
Pro Glu Ile Ala Ala Arg Pro Lys Val Asn Gly Leu Gly Gly Arg Met
225 230 235 240
Glu Phe Ser Trp Thr Leu Leu Asp Val Trp Asp Thr Ile Ser Phe Glu
245 250 255
Ser Thr Gly Asn Leu Val Ala Pro Glu Tyr Gly Phe Lys Ile Ser Lys
260 265 270
Arg Gly Ser Ser Gly Ile Met Lys Thr Glu Gly Thr Leu Glu Asn Cys
275 280 285
Glu Thr Lys Cys Gln Thr Pro Leu Gly Ala Ile Asn Thr Thr Leu Pro
290 295 300
Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Lys Ser Glu Lys Leu Val Leu Ala Thr Gly Pro Arg Asn Val Pro Gln
325 330 335
Ile Glu Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly
340 345 350
Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn
355 360 365
Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Arg Ala
370 375 380
Phe Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn
385 390 395 400
Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Ser Asn Leu Glu Lys Arg
405 410 415
Leu Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp
420 425 430
Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu
435 440 445
Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asn Lys Val Arg Met
450 455 460
Gln Leu Arg Asp Asn Val Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe
465 470 475 480
Tyr His Lys Cys Asp Asp Glu Cys Met Ser Ser Val Lys Asn Gly Thr
485 490 495
Tyr Asp Tyr Pro Lys Tyr Glu Glu Glu Ser Lys Leu Lys Arg Asn Glu
500 505 510
Ile Lys Gly Val Lys Leu Ser Ser Met Gly Val Tyr Gln Ile Leu Ala
515 520 525
Ile Tyr Ala Thr Val Ala Gly Ser Leu Ser Leu Ala Ile Met Val Ala
530 535 540
Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile
545 550 555 560
Cys Ile
<210> 16
<211> 564
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 16
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Asn Ser Thr Ala Thr Leu Cys Leu
20 25 30
Gly His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn
35 40 45
Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Thr
50 55 60
Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln
85 90 95
Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Asn
145 150 155 160
Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Phe Lys Tyr
165 170 175
Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys Leu
180 185 190
Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile Phe
195 200 205
Leu Tyr Ala Gln Ser Gly Arg Ile Thr Val Ser Thr Lys Arg Ser Gln
210 215 220
Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Arg Ile Pro Ser
225 230 235 240
Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly Asp Ile Leu Leu
245 250 255
Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly Tyr Phe Lys Ile
260 265 270
Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala Pro Ile Gly Lys
275 280 285
Cys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile Pro Asn Asp Lys Pro
290 295 300
Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala Cys Pro Arg Tyr Val
305 310 315 320
Lys Gln Thr Leu Lys Leu Ala Thr Gly Met Arg Asn Val Pro Glu Lys
325 330 335
Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly
340 345 350
Trp Glu Gly Met Val Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ser
355 360 365
Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile
370 375 380
Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu Ile Gly Lys Thr Asn Glu
385 390 395 400
Lys Phe His Gln Ile Glu Lys Glu Phe Ser Glu Val Glu Gly Arg Ile
405 410 415
Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp Ser
420 425 430
Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp
435 440 445
Leu Thr Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Lys Lys Gln
450 455 460
Leu Arg Glu Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr
465 470 475 480
His Lys Cys Asp Asn Ala Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr
485 490 495
Asp His Asp Val Tyr Arg Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile
500 505 510
Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile
515 520 525
Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys Val Ala Leu Leu Gly Phe
530 535 540
Ile Met Trp Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Arg Cys
545 550 555 560
Asn Ile Cys Ile
<210> 17
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 17
Met Lys Thr Ile Ile Ala Leu Ser His Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Ile Gly
20 25 30
His His Ala Val Pro Asn Gly Met Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile
50 55 60
Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln
85 90 95
Asn Glu Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Phe Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Ala Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser
145 150 155 160
Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile
195 200 205
Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Ala Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg
225 230 235 240
Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Val Leu Val Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Ser Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Leu Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Lys Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys Tyr Val Glu Asp Ser
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Gly Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Thr Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Lys Arg Phe Gln Ile Lys Gly Ile Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 18
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 18
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Ser Thr Ala Met Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Arg Ile Cys Asp Ser Pro His Arg Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Met Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln
85 90 95
Asn Lys Glu Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Val Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Ala
130 135 140
Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Lys Arg Arg Ser Asn
145 150 155 160
Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Phe Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Ser Asp Gln Ile
195 200 205
Ser Leu Tyr Ala Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg
225 230 235 240
Asn Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Val Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Asn Gly Lys Ser Ser Ile Met Arg Ser Asn Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Val Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Ser Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Val Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Val Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Thr
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Val Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ala Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Ile Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Glu Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe His Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asn Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Arg Cys Ser Ile Cys Ile
565
<210> 19
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 19
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Glu Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Thr Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Glu Ile Cys Asn Ser Pro His Gln Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln
85 90 95
Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala His Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Ile
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Asn
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Lys Phe Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Lys Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Asn Asp Gln Ile
195 200 205
Phe Leu Tyr Ala Gln Ala Ser Gly Arg Ile Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Val Arg
225 230 235 240
Asn Ile Pro Ser Arg Val Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Ala Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Gly Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Met Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Ile Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Ile Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Phe Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile His Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asn Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Glu Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Val Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Ile Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Arg Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 20
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 20
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Ser Ser Ile
50 55 60
Gly Glu Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln
85 90 95
Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Asn
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Phe Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Ile
195 200 205
Phe Leu Tyr Ala Gln Ser Ser Gly Arg Ile Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg
225 230 235 240
Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Lys Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 21
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 21
Met Lys Thr Ile Ile Ala Leu Ser His Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Ile Pro Gly Asn Asp Asn Ser Thr Ala Met Leu Cys Leu Gly
20 25 30
His His Ala Val Ser Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Arg Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Glu Ile Cys Asp Ser Pro His Arg Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln
85 90 95
Asn Lys Glu Trp Asp Leu Phe Val Glu Arg Ser Lys Ala His Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Val Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Lys Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Ala Gln Asn Gly Thr Ser Ser Ala Cys Ile Arg Arg Ser Ser
145 150 155 160
Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Tyr Thr
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Glu Lys Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Asn Asp Gln Ile
195 200 205
Phe Leu Tyr Ala Gln Ala Ser Gly Arg Ile Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Val Arg
225 230 235 240
Asn Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Val Leu Val Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Ser Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys His Ser Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Val Phe Ile Glu Asn Gly Trp Glu Gly Met Met Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Val Gln Asp Leu Glu Lys Tyr Val Glu Asp Ser
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Gly Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile His Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Thr Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Glu Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Lys Arg Phe Gln Ile Lys Gly Ile Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Val Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Arg Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 22
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 22
Met Arg Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Glu Asn Asp Asn Ser Thr Ala Thr Leu Cys Ile Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Val Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Gly Ile Cys Asp Ser Pro His Gln Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Val Asp Ala Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln
85 90 95
Asn Glu Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Phe Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Gln Asp Tyr Ala Ser Leu Arg Ser Leu Ile
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Ala
130 135 140
Gly Val Thr Gln Asn Gly Thr Ser Ser Ala Cys Lys Arg Arg Ser Asn
145 150 155 160
Ser Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Lys Phe Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Lys Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Ser Asp Gln Ile
195 200 205
Ser Leu Tyr Ala Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Ala Ile Pro Asn Ile Gly Ser Arg Pro Arg Ile Arg
225 230 235 240
Asn Ile Pro Ser Arg Val Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Cys Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Asn Gly Lys Ser Ser Ile Met Arg Ser Asn Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Ala Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Gly Lys Pro Phe Gln Asn Val Asn Arg Val Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Ser Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Leu Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Lys Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Ile Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Val Asp Gln Ile Asn Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Thr
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Ile Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Val Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ser Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Met Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu Asp Ile Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asn Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Glu Thr Tyr Asp His Asn Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe His Ile Lys Gly Val Glu Leu Lys Ser Gly Tyr Lys
515 520 525
Asn Trp Ile Leu Trp Ile Ser Phe Ala Thr Ser Cys Phe Leu Leu Cys
530 535 540
Ile Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Lys Cys Asn Ile Cys Ile
565
<210> 23
<211> 573
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 23
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys
50 55 60
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser
130 135 140
Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Ser Ser Phe Phe Arg
145 150 155 160
Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys
165 170 175
Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp Gly
180 185 190
Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Leu Tyr Gln Asn Pro
195 200 205
Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val
210 215 220
Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly Arg Met
225 230 235 240
Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu
245 250 255
Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys
260 265 270
Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys
275 280 285
Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro
290 295 300
Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val
305 310 315 320
Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln
325 330 335
Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser
370 375 380
Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile
385 390 395 400
Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn
405 410 415
Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe
420 425 430
Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn
435 440 445
Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp
450 455 460
Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val
485 490 495
Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu
500 505 510
Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr
515 520 525
Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu Ala
530 535 540
Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Ile Cys Ile Lys Phe Cys Glu Phe Arg Leu
565 570
<210> 24
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 24
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Ile Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Ser Glu Gln Val
20 25 30
Asp Thr Ile Met Glu Arg Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asn Leu Asp Gly Val Lys
50 55 60
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ile Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Thr His Phe Glu
115 120 125
Lys Ile Gln Ile Ile Pro Lys Asn Ser Trp Ser Asp His Glu Ala Ser
130 135 140
Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Arg Ser Ser Phe Phe Arg
145 150 155 160
Asn Val Val Trp Leu Thr Lys Lys Asp Asn Ala Tyr Pro Thr Ile Lys
165 170 175
Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp Gly
180 185 190
Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln Asn
195 200 205
Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu
210 215 220
Val Pro Arg Ile Ala Thr Arg Ser Lys Ile Asn Gly Gln Ser Gly Arg
225 230 235 240
Ile Glu Phe Phe Trp Thr Ile Leu Lys Ser Asn Asp Ala Ile Asn Phe
245 250 255
Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Asn Ala Tyr Lys Ile Val
260 265 270
Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly Asn
275 280 285
Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met
290 295 300
Pro Leu His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr
305 310 315 320
Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro
325 330 335
Gln Gly Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile Ala
340 345 350
Cys Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Ser His His Ser Asn Asp Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu
370 375 380
Ser Thr Gln Arg Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile
385 390 395 400
Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn
405 410 415
Asn Leu Glu Arg Arg Ile Gly Asn Leu Asn Lys Lys Met Glu Asp Gly
420 425 430
Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Gly
435 440 445
Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr
450 455 460
Asp Lys Val Arg Leu Gln Leu Lys Asp Asn Ala Lys Glu Leu Gly Asn
465 470 475 480
Gly Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu Ser
485 490 495
Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg
500 505 510
Leu Arg Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Val
515 520 525
Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Ala Ala Ser Ser Leu Ala Leu
530 535 540
Ala Ile Met Val Ala Gly Leu Phe Leu Trp Met Cys Ser Asn Gly Ser
545 550 555 560
Leu His Ala Asp Cys Ile
565
<210> 25
<211> 568
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 25
Met Glu Lys Ile Val Leu Leu Phe Ala Ile Val Ser Leu Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Lys Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Lys His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Arg
50 55 60
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ala Asn Pro Val Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Ser His Glu Ala Ser
130 135 140
Leu Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe
145 150 155 160
Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile
165 170 175
Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Met Trp
180 185 190
Gly Ile His His Pro Asn Asp Glu Ala Glu Gln Ile Lys Leu Tyr Gln
195 200 205
Asn Pro Thr Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Gln Arg
210 215 220
Leu Thr Pro Arg Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly
225 230 235 240
Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn
245 250 255
Phe Glu Ser Asn Gly Asn Phe Val Ala Pro Glu Tyr Ala Tyr Lys Ile
260 265 270
Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Ser
275 280 285
Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser
290 295 300
Met Pro Phe His His Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys
305 310 315 320
Tyr Val Lys Ser Thr Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser
325 330 335
Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile
340 345 350
Ala Gly Phe Ile Glu Ala Gly Trp Gln Gly Met Val Asp Gly Trp Tyr
355 360 365
Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys
370 375 380
Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser
385 390 395 400
Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Ile Gly Arg Glu Phe
405 410 415
Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp
420 425 430
Gly Phe Leu Asp Val Trp Thr Tyr Lys Ala Glu Leu Leu Val Leu Met
435 440 445
Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Arg Asn Leu
450 455 460
Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Thr Lys Glu Leu Gly
465 470 475 480
Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asp Glu Cys Met Glu
485 490 495
Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala
500 505 510
Arg Leu Asn Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly
515 520 525
Ile Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Val
530 535 540
Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly
545 550 555 560
Ser Leu Gln Cys Arg Val Cys Ile
565
<210> 26
<211> 574
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 26
Met Glu Gln Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser
1 5 10 15
Asp His Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asn Ile
35 40 45
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys
50 55 60
Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Ile Arg Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Leu Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser
130 135 140
Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe
145 150 155 160
Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile
165 170 175
Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp
180 185 190
Gly Ile His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln
195 200 205
Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg
210 215 220
Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly
225 230 235 240
Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile His
245 250 255
Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile
260 265 270
Val Lys Glu Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly
275 280 285
Asn Cys Asn Thr Lys Cys Gln Thr Pro Val Gly Ala Ile Asn Ser Ser
290 295 300
Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys
305 310 315 320
Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser
325 330 335
Pro Leu Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Phe His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Arg Glu
370 375 380
Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile
385 390 395 400
Ile Asp Lys Met Asn Thr Gln Phe Glu Val Val Gly Arg Glu Phe Asn
405 410 415
Asn Leu Glu Ser Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly
420 425 430
Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu
435 440 445
Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr
450 455 460
Asp Arg Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn
465 470 475 480
Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala Arg
500 505 510
Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr
515 520 525
Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu
530 535 540
Ala Ile Met Ile Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser
545 550 555 560
Leu Gln Cys Arg Ile Cys Ile Tyr Phe Cys Glu Phe Arg Leu
565 570
<210> 27
<211> 569
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 27
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys
50 55 60
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser
130 135 140
Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Arg Ser Ser Phe Phe
145 150 155 160
Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile
165 170 175
Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp
180 185 190
Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln
195 200 205
Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg
210 215 220
Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly
225 230 235 240
Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn
245 250 255
Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile
260 265 270
Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly
275 280 285
Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser
290 295 300
Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys
305 310 315 320
Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser
325 330 335
Pro Gln Gly Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile
340 345 350
Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr
355 360 365
Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys
370 375 380
Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser
385 390 395 400
Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe
405 410 415
Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp
420 425 430
Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met
435 440 445
Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu
450 455 460
Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly
465 470 475 480
Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu
485 490 495
Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala
500 505 510
Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly
515 520 525
Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala
530 535 540
Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly
545 550 555 560
Ser Leu Gln Cys Arg Ile Cys Ile Lys
565
<210> 28
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 28
Met Glu Lys Ile Val Leu Leu Phe Ala Ile Val Ser Leu Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Lys Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asn Leu Asp Gly Val Lys
50 55 60
Pro Leu Ile Leu Lys Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ile Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Ile Gln Ile Ile Pro Lys Asn Ser Trp Ser Asp His Glu Ala Ser
130 135 140
Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg
145 150 155 160
Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile Lys
165 170 175
Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly
180 185 190
Ile His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn
195 200 205
Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg Leu
210 215 220
Thr Pro Arg Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly Arg
225 230 235 240
Met Asp Phe Phe Trp Thr Ile Leu Lys Ser Asn Asp Ala Ile Asn Phe
245 250 255
Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Asn Ala Tyr Lys Ile Val
260 265 270
Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly Asn
275 280 285
Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser Met
290 295 300
Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr
305 310 315 320
Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro
325 330 335
Leu Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Phe
355 360 365
His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser
370 375 380
Thr Gln Arg Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile
385 390 395 400
Asn Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn Asn
405 410 415
Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe
420 425 430
Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn
435 440 445
Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp
450 455 460
Lys Val Arg Leu Gln Leu Lys Asp Asn Ala Lys Glu Leu Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu Ser Ile
485 490 495
Arg Asn Gly Thr Tyr Asn Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu
500 505 510
Asn Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Ile Tyr
515 520 525
Gln Ile Leu Ser Ile Tyr Ser Thr Ala Ala Ser Ser Leu Ala Leu Ala
530 535 540
Ile Met Val Ala Gly Leu Phe Leu Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Val Cys Ile
565
<210> 29
<211> 568
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 29
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Ile Val Lys Ser
1 5 10 15
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Lys Gln Val
20 25 30
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile
35 40 45
Leu Glu Lys Lys His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Arg
50 55 60
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn
65 70 75 80
Pro Met Cys Asp Glu Phe Ile Arg Val Pro Glu Trp Ser Tyr Ile Val
85 90 95
Glu Lys Ala Asn Pro Val Asn Asp Leu Cys Tyr Pro Gly Val Phe Asn
100 105 110
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu
115 120 125
Lys Leu Gln Ile Ile Pro Lys Ser Ser Trp Ser Ser His Glu Ala Ser
130 135 140
Leu Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe
145 150 155 160
Arg Asn Val Val Trp Leu Thr Lys Lys Asp Asn Ala Tyr Pro Thr Ile
165 170 175
Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Met Trp
180 185 190
Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln
195 200 205
Asn Pro Thr Thr Tyr Val Ser Val Gly Thr Ser Thr Leu Asn Gln Arg
210 215 220
Leu Val Pro Arg Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly
225 230 235 240
Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Ser Asn Asp Val Ile Asn
245 250 255
Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile
260 265 270
Val Lys Glu Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Ser
275 280 285
Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser
290 295 300
Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys
305 310 315 320
Tyr Val Lys Ser Thr Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser
325 330 335
Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile
340 345 350
Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr
355 360 365
Gly Phe His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Arg
370 375 380
Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser
385 390 395 400
Ile Ile Asn Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe
405 410 415
Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp
420 425 430
Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met
435 440 445
Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu
450 455 460
Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly
465 470 475 480
Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu
485 490 495
Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala
500 505 510
Arg Leu Asn Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly
515 520 525
Val Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala
530 535 540
Leu Ala Ile Met Ile Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly
545 550 555 560
Ser Leu Gln Cys Arg Ile Cys Ile
565
<210> 30
<211> 472
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 30
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr Cys
35 40 45
Thr Ile Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val
50 55 60
Asn Gln Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln
65 70 75 80
Ser Val Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val
85 90 95
Ser Gly Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser
100 105 110
Lys Gly Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Pro
115 120 125
Leu Glu Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp
130 135 140
Lys His Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu
145 150 155 160
Met Ser Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe
165 170 175
Glu Ser Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp
180 185 190
Leu Thr Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu
195 200 205
Lys Tyr Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn
210 215 220
Ile Leu Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys
225 230 235 240
Phe Thr Ile Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys
245 250 255
Ile Phe Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn
260 265 270
Ala Pro Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser
275 280 285
Glu Ile Thr Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro
290 295 300
Trp Val Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys
305 310 315 320
Ser Gly Val Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser
325 330 335
Cys Gly Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser
340 345 350
Phe Lys Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser
355 360 365
Ser Arg Lys Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly
370 375 380
Thr Asp Asn Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Asn Glu Trp
385 390 395 400
Ser Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu
405 410 415
Asp Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro
420 425 430
Lys Glu Asn Thr Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys
435 440 445
Gly Val Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu
450 455 460
Leu Pro Phe Thr Ile Asp Lys Tyr
465 470
<210> 31
<211> 470
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 31
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Ser Ile Ala
1 5 10 15
Ile Gly Ile Ile Ser Leu Met Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ala Ser His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile
35 40 45
Cys Asn Gln Arg Ile Ile Thr Tyr Glu Asn Ser Thr Trp Val Asn His
50 55 60
Thr Tyr Val Ser Ile Asn Asn Thr Asn Val Val Ala Gly Lys Asp Lys
65 70 75 80
Thr Ser Val Thr Leu Ala Gly Asn Ser Ser Leu Cys Ser Ile Ser Gly
85 90 95
Trp Ala Ile Tyr Thr Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Lys Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met Ser
145 150 155 160
Cys Pro Leu Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Ile Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asp Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Gly Thr Ile Lys Ser Trp Lys Lys Gln Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile
275 280 285
Thr Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Pro Glu Asp Gly Glu Gly Ser Cys Asn
325 330 335
Pro Val Thr Val Asp Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Lys
340 345 350
Tyr Asp Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Asn Arg Leu Arg
355 360 365
Lys Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Asn Thr Asp
370 375 380
Ser Asp Phe Ser Val Lys Gln Asp Val Val Ala Ile Thr Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln Tyr Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Met Arg Pro Cys Phe Trp Val Glu Leu Val Arg Gly Leu Pro Arg
420 425 430
Glu Asn Thr Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly
435 440 445
Val Asn Ser Asp Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Glu Leu
450 455 460
Pro Phe Thr Ile Asp Lys
465 470
<210> 32
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 32
Met Asn Pro Thr Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Ile Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val
65 70 75 80
Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Arg Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Ser Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Arg His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro His Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Lys Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Glu Thr Ile Lys Ser Trp Lys Lys Arg Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asn Gly Ala Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala Pro
260 265 270
Asn Phe His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Thr Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Gly Ala Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly
325 330 335
Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Asn Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Asn Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Lys Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Ile Gln His Pro Glu Leu Thr Gly Leu Asn
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asp Ser Asp Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Ile Ile Asp Lys
465
<210> 33
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 33
Met Asn Pro Asn Lys Lys Ile Ile Thr Ile Gly Ser Ile Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Met Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Val Ser His Ser Ile Gln Ile Gly Asn Gln Ser Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn His
50 55 60
Thr Tyr Val Asn Ile Asn Asn Thr Asn Val Val Ala Gly Glu Asp Lys
65 70 75 80
Thr Ser Val Thr Leu Ala Gly Asn Ser Ser Leu Cys Pro Ile Arg Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Met Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Thr Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Thr Ser Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Ser Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Ile Lys Ser Val Glu Met Lys Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Ile Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Met Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Pro Lys Asp Gly Lys Gly Ser Cys Asn
325 330 335
Pro Val Thr Val Asp Gly Ala Asp Gly Val Lys Gly Phe Ser Tyr Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Lys Gly Phe Glu Met Ile Trp Asp Thr Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Lys Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Asn Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Glu
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ile Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Thr Val Asp Lys
465
<210> 34
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 34
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val
65 70 75 80
Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile
275 280 285
Thr Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly
325 330 335
Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Lys Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Asn Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Asn Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 35
<211> 470
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 35
Met Asn Pro Thr Gln Lys Ile Ile Thr Ile Gly Ser Ile Ser Ile Ala
1 5 10 15
Ile Gly Ile Ile Ser Leu Met Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ala Ser His Ser Ile Gln Thr Gly Ser Gln Asn His Thr Gly Ile
35 40 45
Cys Asn Gln Arg Ile Ile Thr Tyr Glu Asn Ser Thr Trp Val Asn His
50 55 60
Thr Tyr Val Asn Ile Asn Asn Thr Asn Val Val Ala Gly Lys Asp Lys
65 70 75 80
Thr Ser Val Thr Leu Ala Gly Asn Ser Ser Leu Cys Pro Ile Arg Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Met Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Thr Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met Ser
145 150 155 160
Cys Pro Leu Gly Glu Ala Pro Ser Pro Tyr Asn Ser Lys Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Ser Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Gly Thr Ile Lys Ser Trp Lys Lys Gln Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Met Thr Asp Gly Pro Ser Asn Gly Ala Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala Pro
260 265 270
Asn Phe His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Thr Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Gly Ala Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Pro Glu Asp Gly Glu Gly Ser Cys Asn
325 330 335
Pro Val Thr Val Asp Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Asn Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Asn Thr Asp
370 375 380
Ser Asp Phe Ser Val Lys Gln Asp Val Val Ala Ile Thr Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Ile Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Met Arg Pro Cys Phe Trp Val Glu Leu Val Arg Gly Leu Pro Arg
420 425 430
Glu Asn Thr Thr Ile Trp Thr Ser Gly Ser Ser Met Ser Phe Cys Gly
435 440 445
Val Asn Ser Asp Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Glu Leu
450 455 460
Pro Phe Ile Ile Asp Lys
465 470
<210> 36
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 36
Met Asn Pro Asn Lys Lys Ile Ile Thr Ile Gly Ser Ile Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Met Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Val Ser His Ser Ile Gln Ile Gly Asn Gln Ser Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Ile Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn His
50 55 60
Thr Tyr Val Ser Ile Asn Asn Thr Asn Val Val Ala Gly Glu Asp Lys
65 70 75 80
Thr Ser Val Thr Leu Ala Gly Asn Ser Ser Leu Cys Ser Ile Ser Gly
85 90 95
Trp Ala Ile Tyr Thr Lys Asp Asn Ser Ile Arg Ile Gly Ser Arg Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Lys Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Arg His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro His Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Thr Ser Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asp Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Glu Thr Ile Lys Ser Trp Lys Lys Arg Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Ile Lys Ser Val Glu Met Lys Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Ile Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Ile
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Met Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Pro Lys Asp Gly Lys Gly Ser Cys Asn
325 330 335
Pro Val Thr Val Asp Gly Ala Asp Gly Val Lys Gly Phe Ser Tyr Lys
340 345 350
Tyr Asp Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Asn Arg Leu Arg
355 360 365
Lys Gly Phe Glu Met Ile Trp Asp Ser Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Lys Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Lys Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Ile Gln His Pro Glu Leu Thr Gly Leu Asn
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Glu
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asp Ser Asp Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Thr Val Asp Lys
465
<210> 37
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 37
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Thr Thr Ile Ile Glu Arg Asn Ile Thr
50 55 60
Glu Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro
65 70 75 80
Lys Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Val His
130 135 140
Ser Asn Asn Thr Val Arg Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Tyr Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Thr Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Ile Lys Asp His Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Ser His
325 330 335
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu
355 360 365
Thr Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Asn Pro Lys Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu
420 425 430
Glu Thr Glu Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Leu
450 455 460
Asn Leu Met Pro Ile
465
<210> 38
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 38
Met Asn Pro Asn Gln Lys Ile Ile Ala Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln Tyr Glu Cys Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Pro Cys Glu Pro Ile Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Asn Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Ser Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Ile Pro His Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly His Asp Glu Asn Ala Thr Ala Ser Phe Ile Tyr Asp
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Lys Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Ser Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Arg Ser Ser Ser Ser Tyr
325 330 335
Cys Arg Asn Pro Asn Asn Glu Lys Gly Ser His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asn Gly Asp Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Leu Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Glu Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Glu Gln
420 425 430
Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Ile Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Leu Met Pro Ile
465
<210> 39
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 39
Met Asn Pro Asn Lys Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Glu Cys Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Met Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Ile Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Gly Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Arg Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asn Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asn Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Val His
130 135 140
Ser Asn Asn Thr Val Arg Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Ser Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Met
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Ile Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Asp Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asp Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Asn Ala Thr Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Lys Glu Gly Lys Ile Val His Thr Ser Lys Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg
275 280 285
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Ile Lys Asp His Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Ser Asp Ser Ser Ser Ser Ser His
325 330 335
Cys Leu Asn Pro Asn Asn Glu Glu Gly Gly His Arg Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asp Asp Val Trp Met Gly Arg Thr Ile Asn Glu
355 360 365
Thr Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Glu Gly Trp Ser
370 375 380
Asn Pro Lys Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Ser Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Gln
420 425 430
Glu Thr Glu Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Leu
450 455 460
Asn Leu Met His Ile
465
<210> 40
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 40
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Val Cys Phe Leu Met Gln Ile Ala Ile Leu Ala Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Val Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Val Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asp Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Gly Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Arg His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Ile Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys Tyr Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Val Ser Trp Ser Lys Glu Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Ala Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Thr Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Arg Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Ile Lys Asp Tyr Ser Ile Val Ser Arg Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Ser His
325 330 335
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Asn Pro Lys Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Phe Val Glu Leu Ile Arg Gly Arg Lys Glu
420 425 430
Glu Thr Glu Val Leu Trp Thr Ser Asn Ser Ile Val Val Leu Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ala Gly Ser Trp Pro Asp Gly Ala Asp Leu
450 455 460
Asn Leu Met Pro Ile
465
<210> 41
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 41
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ser Thr Ile Cys Phe Phe Met Gln Ile Ala Ile Leu Ile Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln Tyr Glu Phe Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Gly Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Val His
130 135 140
Ser Asn Asn Thr Val Arg Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Glu Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Thr Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Ile Lys Asp His Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Ser His
325 330 335
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu
355 360 365
Thr Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Asn Pro Lys Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu
420 425 430
Glu Thr Glu Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Leu
450 455 460
Asn Leu Met Pro Ile
465
<210> 42
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 42
Met Asn Pro Asn Gln Lys Ile Ile Ser Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln Tyr Glu Cys Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Pro Cys Glu Pro Ile Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Asn Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asp Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Arg Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asn Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Gly Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Ser Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys Tyr Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly Asp Asp Glu Asn Ala Thr Ala Ser Phe Ile Tyr Asp
195 200 205
Gly Arg Leu Val Asp Ser Val Val Ser Trp Ser Lys Asp Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asp Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Asn Ala Thr Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Lys Glu Gly Lys Ile Val His Thr Ser Lys Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg
275 280 285
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Ser Asp Ser Ser Ser Ser Ser His
325 330 335
Cys Leu Asn Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asn Gly Asp Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Glu Gly Trp Ser
370 375 380
Asn Pro Lys Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Ser Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Phe Val Glu Leu Ile Arg Gly Arg Lys Gln
420 425 430
Glu Thr Glu Val Trp Trp Thr Ser Asn Ser Ile Val Val Leu Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ala Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Leu Met Pro Ile
465
<210> 43
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 43
Met Asn Pro Asn Lys Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Val Cys Phe Leu Met Gln Ile Ala Ile Leu Ala Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Glu Cys Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Val Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Met Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Ile Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Val Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Val Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Ala Arg Glu Pro Tyr Val Ser Cys Asp Pro Asn Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Arg His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Ile Pro His Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Ile Pro Phe His Leu Gly Thr Lys Gln Val Cys Met
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Ile Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Lys Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Ala Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Ile Lys Asp Tyr Ser Ile Val Ser Arg Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Arg Ser Ser Ser Ser Tyr
325 330 335
Cys Arg Asn Pro Asn Asn Glu Lys Gly Ser His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asp Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Leu Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Glu Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Glu Gln
420 425 430
Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Ile Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Pro Trp Pro Asp Gly Ala Asp Leu
450 455 460
Asn Leu Met His Ile
465
<210> 44
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 44
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Ala
130 135 140
Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile
145 150 155 160
Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro Lys
165 170 175
Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr
260 265 270
Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 45
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 45
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Ala
130 135 140
Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn Ile
145 150 155 160
Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro Lys
165 170 175
Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr
260 265 270
Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 46
<211> 585
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 46
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Ser Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Ser Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Ile Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Ile Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Pro Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val
85 90 95
Ser Ile Leu His Glu Ala Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asp
130 135 140
Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val
180 185 190
Pro Tyr Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe
195 200 205
His Ser Asp Asn Glu Ala Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys
210 215 220
Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Ala Thr His Tyr Val
225 230 235 240
Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro
245 250 255
Gln Ser Gly Arg Ile Ile Val Asp Tyr Met Val Gln Lys Pro Gly Lys
260 265 270
Thr Gly Thr Ile Val Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val
275 280 285
Trp Cys Thr Ser Gly Arg Ser Lys Val Ile Lys Gly Phe Leu Pro Leu
290 295 300
Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys
305 310 315 320
Ser Arg Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Val Gly Asn Cys
325 330 335
Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr
340 345 350
Arg Pro Pro Ala Lys Leu Leu Lys Glu Lys Gly Phe Phe Gly Ala Ile
355 360 365
Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Val Ala Gly Trp His
370 375 380
Gly Tyr Thr Ser His Gly Ala His Gly Ile Ala Val Ala Ala Asp Leu
385 390 395 400
Lys Ser Thr Gln Glu Ala Ile Asn Arg Ile Thr Lys Asn Leu Asn Ser
405 410 415
Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Ser Ala Met
420 425 430
Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp
435 440 445
Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Ala Glu Ala Val His Phe
450 455 460
Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Met Ala Leu
465 470 475 480
Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly
485 490 495
Asn Gly Cys Phe Glu Thr Gln His Lys Cys Asn Gln Thr Cys Leu Asp
500 505 510
Lys Ile Ala Ala Gly Thr Phe Asp Ala Glu Glu Phe Ser Leu Pro Thr
515 520 525
Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asp Asp Asp Gly Leu
530 535 540
Asp Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu
545 550 555 560
Ala Val Thr Leu Met Ile Ala Ile Phe Ile Val Tyr Met Ile Ser Arg
565 570 575
Asp Asn Val Ser Cys Ser Ile Cys Leu
580 585
<210> 47
<211> 583
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 47
Met Lys Ala Met Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Ile Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Lys Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Asn Ile Pro Ser Ala Arg Val
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Thr Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asn
130 135 140
Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Val Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Asp Lys Asn Lys Thr Ala Thr Asn Ser Leu Thr Ile Glu Val Pro Tyr
180 185 190
Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Ile Trp Gly Phe His Ser
195 200 205
Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Asn Pro Gln
210 215 220
Lys Phe Thr Ser Ser Ala Asn Gly Ile Thr Thr His Tyr Val Ser Gln
225 230 235 240
Ile Gly Gly Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln Ser
245 250 255
Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys Thr Gly
260 265 270
Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp Cys
275 280 285
Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile Gly
290 295 300
Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser Lys
305 310 315 320
Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro Ile
325 330 335
Trp Val Lys Thr Pro Leu Thr Leu Ala Asn Gly Thr Lys Tyr Arg Pro
340 345 350
Pro Ala Lys Leu Leu Arg Glu Arg Gly Phe Phe Gly Ala Ile Ala Gly
355 360 365
Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly Tyr
370 375 380
Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys Ser
385 390 395 400
Thr Gln Glu Ala Ile Asn Lys Ile Thr Gln Asn Leu Asn Ser Leu Ser
405 410 415
Glu Leu Glu Ile Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp Glu
420 425 430
Leu His Asn Glu Ile Ile Glu Leu Asp Glu Lys Val Asp Asp Leu Arg
435 440 445
Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Thr Val Leu Leu Ser Asn
450 455 460
Glu Gly Ile Ile Asn Asn Glu Asp Glu His Leu Leu Ala Leu Glu Arg
465 470 475 480
Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Glu Ile Gly Asn Gly
485 490 495
Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Ile Asp Arg Ile
500 505 510
Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe Asp
515 520 525
Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp Asn
530 535 540
His Thr Ile Met Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val
545 550 555 560
Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp Asn
565 570 575
Phe Ser Cys Ser Ile Cys Leu
580
<210> 48
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 48
Met Lys Thr Ile Ile Val Leu Leu Met Val Val Thr Ser Lys Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Ala Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Ala Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Ala Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Asn Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ser Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Asn Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Arg Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Ala Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Thr Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Ile Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asn
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Arg Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Glu Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Val Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Val Cys Leu
580
<210> 49
<211> 585
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 49
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Glu Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Lys Ile Pro Ser Ala Arg Val
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu His Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu Asp
130 135 140
Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ile Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asn Asp Lys Asn Lys Thr Ala Thr Asn Pro Leu Thr Ile Glu Val
180 185 190
Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe
195 200 205
His Ser Asp Asn Glu Thr Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys
210 215 220
Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val
225 230 235 240
Ser Gln Ile Gly Gly Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro
245 250 255
Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Ser Gly Lys
260 265 270
Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val
275 280 285
Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu
290 295 300
Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys
305 310 315 320
Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys
325 330 335
Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr
340 345 350
Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile
355 360 365
Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His
370 375 380
Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu
385 390 395 400
Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser
405 410 415
Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met
420 425 430
Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp
435 440 445
Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu
450 455 460
Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu
465 470 475 480
Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly
485 490 495
Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp
500 505 510
Arg Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr
515 520 525
Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu
530 535 540
Asp Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu
545 550 555 560
Ala Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg
565 570 575
Asp Asn Val Ser Cys Ser Ile Cys Leu
580 585
<210> 50
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 50
Met Lys Thr Ile Ile Val Leu Leu Met Val Val Thr Ser Ser Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Ile Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Ala Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Ala Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Asn Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ser Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Asn Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro
180 185 190
Tyr Val Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Ile Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Gly Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Arg Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Ile Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Gln Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Ile Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asn
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Met Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Ile Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asp Asp Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 51
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 51
Met Lys Thr Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Lys Ala
1 5 10 15
Asp Arg Ile Cys Thr Gly Ile Thr Ser Ser Ser Ser Pro His Val Val
20 25 30
Lys Thr Ala Thr Gln Gly Glu Ile Asn Val Thr Gly Val Ile Pro Leu
35 40 45
Thr Thr Thr Pro Ile Lys Ser His Phe Ala Asn Leu Lys Gly Thr Lys
50 55 60
Thr Arg Gly Lys Leu Cys Pro Lys Cys Pro Asn Cys Thr Asp Leu Asp
65 70 75 80
Val Ala Leu Gly Arg Pro Lys Cys Thr Gly Asn Ile Pro Ser Ala Arg
85 90 95
Val Ser Ile Leu His Glu Ala Arg Pro Val Thr Ser Gly Cys Phe Pro
100 105 110
Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg
115 120 125
Gly Tyr Glu His Ile Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu
130 135 140
Asn Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro
145 150 155 160
Asn Val Thr Asn Gly Asn Gly Phe Phe Ala Thr Met Ala Trp Ala Val
165 170 175
Pro Asp Lys Asn Lys Thr Ala Thr Asn Ser Leu Thr Ile Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Ile Trp Gly Phe His
195 200 205
Ser Asp Asn Glu Ala Gln Met Ala Lys Leu Tyr Gly Asp Ser Lys Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Ala Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Glu Tyr Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Lys Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Ser Ala Met Asp
420 425 430
Glu Leu His Asn Glu Ile Ile Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Met Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Glu Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys
500 505 510
Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Glu Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Ile Ala Ile Phe Ile Val Tyr Met Ile Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 52
<211> 586
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 52
Met Lys Thr Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala
1 5 10 15
Asp Arg Val Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val
20 25 30
Lys Thr Ala Thr Gln Gly Glu Ile Asn Val Thr Gly Val Ile Pro Leu
35 40 45
Thr Thr Thr Pro Thr Arg Ser His Phe Ala Asn Leu Lys Gly Thr Lys
50 55 60
Thr Arg Gly Lys Leu Cys Pro Thr Cys Leu Asn Cys Thr Asp Leu Asp
65 70 75 80
Val Ala Leu Gly Arg Pro Met Cys Val Gly Ile Thr Pro Ser Ala Lys
85 90 95
Ala Ser Ile Leu His Glu Val Lys Pro Val Thr Ser Gly Cys Phe Pro
100 105 110
Ile Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg
115 120 125
Gly Tyr Glu Asn Val Arg Leu Ser Thr His Asn Val Ile Asn Ala Glu
130 135 140
Gly Ala Pro Gly Gly Pro Tyr Lys Ile Gly Thr Ser Gly Ser Cys Pro
145 150 155 160
Asn Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val
165 170 175
Pro Lys Asn Asp Asn Asn Lys Thr Ala Thr Asn Pro Leu Thr Val Glu
180 185 190
Val Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly
195 200 205
Phe His Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser
210 215 220
Asn Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Met Thr Thr His Tyr
225 230 235 240
Val Ser Gln Ile Gly Ser Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu
245 250 255
Pro Gln Ser Gly Arg Ile Ile Val Asp Tyr Met Val Gln Lys Ser Arg
260 265 270
Lys Thr Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys
275 280 285
Val Trp Cys Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro
290 295 300
Leu Ile Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn
305 310 315 320
Lys Ser Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Val Gly Asn
325 330 335
Cys Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys
340 345 350
Tyr Arg Pro Pro Ala Lys Leu Leu Arg Glu Arg Gly Phe Phe Gly Ala
355 360 365
Ile Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp
370 375 380
His Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp
385 390 395 400
Leu Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn
405 410 415
Ser Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Ile Ser Gly Ala
420 425 430
Met Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp
435 440 445
Asp Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu
450 455 460
Leu Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Met Ala
465 470 475 480
Leu Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Glu Ile
485 490 495
Gly Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Ile
500 505 510
Asp Arg Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro
515 520 525
Thr Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly
530 535 540
Leu Asp Ser His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser
545 550 555 560
Leu Ala Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Ile Ser
565 570 575
Arg Asp Asn Val Ser Cys Ser Ile Cys Leu
580 585
<210> 53
<211> 585
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 53
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Val
180 185 190
Pro Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe
195 200 205
His Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn
210 215 220
Pro Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val
225 230 235 240
Ser Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro
245 250 255
Gln Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Lys
260 265 270
Thr Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val
275 280 285
Trp Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu
290 295 300
Ile Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys
305 310 315 320
Ser Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys
325 330 335
Pro Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr
340 345 350
Arg Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile
355 360 365
Ala Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His
370 375 380
Gly Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu
385 390 395 400
Lys Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser
405 410 415
Leu Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met
420 425 430
Asp Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp
435 440 445
Leu Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu
450 455 460
Ser Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu
465 470 475 480
Glu Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly
485 490 495
Asn Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp
500 505 510
Arg Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr
515 520 525
Phe Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu
530 535 540
Asp Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu
545 550 555 560
Ala Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg
565 570 575
Asp Asn Val Ser Cys Ser Ile Cys Leu
580 585
<210> 54
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 54
Met Lys Ala Ile Leu Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro Tyr Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Ala Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Lys Thr
50 55 60
Arg Gly Lys Leu Cys Pro Lys Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Lys Cys Thr Gly Asn Ile Pro Ser Ala Glu Val
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Ala Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Asn Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Lys Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ser Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Asn Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro
180 185 190
Tyr Ile Cys Ala Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Gly Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Ser Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Thr Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Arg Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Val Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Val Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asp Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Val Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ser Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Thr
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Ile Ser Cys Ser Ile Cys Leu
580
<210> 55
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 55
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asp Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Ile Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Ile Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Ile Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Met Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Ile Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Arg Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Ile Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asp Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Met Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Arg Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Glu Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ala Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asp Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Glu Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Ala Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Lys Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Ile Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Arg Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Ser Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Val Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Lys Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Thr His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Arg Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Tyr Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Ala Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Val Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 56
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 56
Met Lys Val Ile Ile Val Leu Leu Met Val Val Met Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Ser Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Ile Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Lys Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Phe Leu Arg Gly
115 120 125
Tyr Glu Lys Ile Arg Leu Ser Thr Lys Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Arg Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Val Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asn Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Arg Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Ser Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Phe Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Lys Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Thr Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Ser Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Leu Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Phe Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Met Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Gln Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asn Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Val Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 57
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 57
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Thr Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Leu Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Lys Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Gly Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Val Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Ser Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Asp Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Arg Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Glu Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asn Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 58
<211> 583
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 58
Met Lys Val Ile Ile Val Leu Leu Met Val Val Thr Ser Asp Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Ile Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Thr Lys Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Phe Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Met Gly Thr Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Lys Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Ala Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Asn Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Arg Gly Pro Tyr Arg Leu Gly Thr Ser Arg Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Ser Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Asn Asn Lys Asn Ala Thr Asn Pro Leu Thr Val Glu Val Pro
180 185 190
Tyr Ile Cys Ala Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asp Lys Thr Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Arg Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Gly Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Met Gln Lys Pro Gly Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Val Leu Phe Pro Gln Lys Val Trp
275 280 285
Cys Ala Ser Gly Lys Ser Lys Val Ile Lys Gly Ser Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Glu His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Ser Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Val Glu Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Lys
500 505 510
Ile Ala Ala Gly Thr Phe Asn Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asn Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Thr
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Val Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys
580
<210> 59
<211> 583
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 59
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Met Ser Asn Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Thr Thr Pro Thr Lys Ser His Phe Ala Asn Leu Lys Gly Thr Lys Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Ile Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Thr Ile Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Lys Ile Arg Leu Ser Thr Lys Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Arg Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Ala Thr Ser Lys Val Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asn Asn Tyr Lys Asn Ala Thr Asn Pro Gln Thr Val Glu Ile Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Ile Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Ser Phe Pro Asp Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Glu Lys Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Val Ser Gly Arg Ser Lys Val Ile Lys Gly Ala Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Asp Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Asp Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Glu Asp Gly Leu Asp
530 535 540
Asn His Thr Ile Leu Leu Tyr Tyr Ser Thr Val Ala Ser Ser Leu Ala
545 550 555 560
Val Thr Leu Met Ile Ala Ile Phe Val Val Tyr Met Val Ser Arg Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys
580
<210> 60
<211> 584
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 60
Met Lys Ala Ile Ile Val Leu Leu Met Val Val Ala Ser Asp Ala Asp
1 5 10 15
Arg Ile Cys Thr Gly Ile Thr Ser Ser Asn Ser Pro His Val Val Lys
20 25 30
Thr Ala Thr Gln Gly Glu Val Asn Val Thr Gly Val Ile Pro Leu Thr
35 40 45
Ile Thr Pro Thr Lys Ser Tyr Phe Ala Asn Leu Lys Gly Ile Arg Thr
50 55 60
Arg Gly Lys Leu Cys Pro Asp Cys Phe Asn Cys Thr Asp Leu Asp Val
65 70 75 80
Ala Leu Gly Arg Pro Met Cys Val Gly Ala Thr Pro Ser Ala Lys Ala
85 90 95
Ser Ile Leu His Glu Val Arg Pro Val Thr Ser Gly Cys Phe Pro Ile
100 105 110
Met His Asp Arg Thr Lys Ile Arg Gln Leu Pro Asn Leu Leu Arg Gly
115 120 125
Tyr Glu Asn Ile Arg Leu Ser Thr Gln Asn Val Ile Asp Ala Glu Lys
130 135 140
Ala Pro Arg Gly Pro Tyr Arg Leu Gly Thr Ser Gly Ser Cys Pro Asn
145 150 155 160
Thr Thr Ser Lys Ile Gly Phe Phe Ala Thr Met Ala Trp Ala Val Pro
165 170 175
Lys Asp Lys Tyr Lys Asn Ala Thr Asn Pro Gln Thr Leu Glu Ile Pro
180 185 190
Tyr Ile Cys Thr Glu Gly Glu Asp Gln Ile Thr Val Trp Gly Phe His
195 200 205
Ser Asp Asn Lys Thr Gln Met Lys Asn Leu Tyr Gly Asp Ser Asn Pro
210 215 220
Gln Lys Phe Thr Ser Ser Ala Asn Gly Val Thr Thr His Tyr Val Ser
225 230 235 240
Gln Ile Gly Asp Phe Pro Asn Gln Thr Glu Asp Gly Gly Leu Pro Gln
245 250 255
Ser Gly Arg Ile Val Val Asp Tyr Met Val Gln Lys Pro Gly Arg Thr
260 265 270
Gly Thr Ile Val Tyr Gln Arg Gly Ile Leu Leu Pro Gln Lys Val Trp
275 280 285
Cys Ala Asn Gly Arg Ser Lys Val Ile Lys Gly Ala Leu Pro Leu Ile
290 295 300
Gly Glu Ala Asp Cys Leu His Glu Lys Tyr Gly Gly Leu Asn Lys Ser
305 310 315 320
Lys Pro Tyr Tyr Thr Gly Lys His Ala Lys Ala Ile Gly Asn Cys Pro
325 330 335
Ile Trp Val Lys Thr Pro Leu Lys Leu Ala Asn Gly Thr Lys Tyr Arg
340 345 350
Pro Pro Ala Lys Leu Leu Lys Glu Arg Gly Phe Phe Gly Ala Ile Ala
355 360 365
Gly Phe Leu Glu Gly Gly Trp Glu Gly Met Ile Ala Gly Trp His Gly
370 375 380
Tyr Thr Ser His Gly Ala His Gly Val Ala Val Ala Ala Asp Leu Lys
385 390 395 400
Ser Thr Gln Glu Ala Ile Asn Lys Ile Thr Lys Asn Leu Asn Ser Leu
405 410 415
Ser Glu Leu Glu Val Lys Asn Leu Gln Arg Leu Ser Gly Ala Met Asp
420 425 430
Glu Leu His Ser Glu Ile Leu Glu Leu Asp Glu Lys Val Asp Asp Leu
435 440 445
Arg Ala Asp Thr Ile Ser Ser Gln Ile Glu Leu Ala Val Leu Leu Ser
450 455 460
Asn Glu Gly Ile Ile Asn Ser Glu Asp Glu His Leu Leu Ala Leu Glu
465 470 475 480
Arg Lys Leu Lys Lys Met Leu Gly Pro Ser Ala Ile Asp Ile Gly Asn
485 490 495
Gly Cys Phe Glu Thr Lys His Lys Cys Asn Gln Thr Cys Leu Asp Arg
500 505 510
Ile Ala Ala Gly Thr Phe Ser Ala Gly Glu Phe Ser Leu Pro Thr Phe
515 520 525
Asp Ser Leu Asn Ile Thr Ala Ala Ser Leu Asn Asp Asp Gly Leu Asp
530 535 540
Asn His Ala Ile Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Thr
545 550 555 560
Val Thr Leu Met Leu Ala Ile Phe Ile Val Tyr Met Val Ser Lys Asp
565 570 575
Asn Val Ser Cys Ser Ile Cys Leu
580
<210> 61
<211> 467
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 61
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Pro Thr Glu Ile Thr Ala Pro
35 40 45
Thr Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Leu Leu Leu Leu Pro Glu Pro Glu
65 70 75 80
Trp Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala
85 90 95
Leu Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala
100 105 110
Pro Leu Ile Ile Arg Glu Pro Phe Val Ala Cys Gly Pro Asn Glu Cys
115 120 125
Lys His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr
130 135 140
Asn Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val
145 150 155 160
Lys Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala
165 170 175
Ala Trp Ser Gly Ser Ala Cys His Asp Gly Lys Glu Trp Thr Tyr Ile
180 185 190
Gly Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Val Lys Tyr Gly
195 200 205
Glu Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asn Cys Tyr Leu Met
225 230 235 240
Ile Thr Asp Gly Ser Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys
245 250 255
Ile Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val
260 265 270
Lys His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile
275 280 285
Glu Cys Ala Cys Arg Asp Asn Arg Tyr Thr Ala Lys Arg Pro Phe Val
290 295 300
Lys Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr
305 310 315 320
Asp Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly
325 330 335
Pro Cys Glu Ser Asp Gly Asp Lys Gly Ser Gly Gly Ile Lys Gly Gly
340 345 350
Phe Val His Gln Arg Met Lys Ser Lys Ile Gly Arg Trp Tyr Ser Arg
355 360 365
Thr Met Ser Lys Thr Glu Arg Met Gly Met Gly Leu Tyr Val Lys Tyr
370 375 380
Gly Gly Asp Pro Trp Ala Asp Ser Asp Ala Leu Ala Phe Ser Gly Val
385 390 395 400
Met Val Ser Met Lys Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile
405 410 415
Lys Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His
420 425 430
Asp Gly Gly Lys Glu Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys
435 440 445
Leu Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asp
450 455 460
Met Ala Leu
465
<210> 62
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 62
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Val Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Leu Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Arg Thr Glu Ile Thr Ala Pro
35 40 45
Ile Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Leu Leu Leu Pro Gly Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Gln Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ala Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Asn
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Ser Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Lys Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Ile Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Lys Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Glu
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Ser Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Ile Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Glu
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asp Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asn Gly Asp Lys Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Glu Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Glu Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Ala Asp Ser Gly Ala Leu Ala Phe Ser Gly Val Met
385 390 395 400
Val Ser Met Lys Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Arg
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Arg Glu Thr Trp His Ser Ala Ala Thr Thr Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Thr Leu
465
<210> 63
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 63
Met Leu Ser Ser Thr Ile Gln Thr Leu Thr Leu Leu Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Gln Thr Glu Ile Thr Ala Pro
35 40 45
Ile Met Ser Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Ala Lys Gly Val Thr Leu Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Ile Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ile Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Thr Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Ser
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Thr Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ser Cys His Asp Gly Lys Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Lys Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Ile Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Glu Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Asn Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Thr Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asp Lys Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Lys Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Glu Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Lys Thr Thr Trp His Ser Ala Ala Thr Ala Val Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asp Met
450 455 460
Ala Leu
465
<210> 64
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 64
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Ile Tyr Val Ser Ala Ser Leu Ser Tyr Leu Ile
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Pro Thr Glu Ile Thr Ala Pro
35 40 45
Thr Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val His Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Phe Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Val Ala Cys Gly Pro Asn Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Thr Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Glu
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Thr Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Lys Glu Trp Thr Tyr Val Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Val Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Arg Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Ser Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Gln Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Asn Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Arg Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Ser Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Asp
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asn Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Lys Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Gln Thr Glu Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Gly
370 375 380
Gly Asp Pro Trp Ala Asp Ser Asp Ala Leu Ala Phe Ser Gly Val Met
385 390 395 400
Ile Ser Met Lys Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Met Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Val Glu Met Val His Asp
420 425 430
Gly Gly Lys Glu Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Lys Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Ala Leu
465
<210> 65
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 65
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Pro Thr Glu Ile Thr Ala Pro
35 40 45
Thr Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Leu Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Val Ala Cys Gly Pro Asn Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Lys Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Val Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Ser Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Arg Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Asp
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asp Lys Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Lys Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Glu Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Gly
370 375 380
Gly Asp Pro Trp Ala Asp Ser Asp Ala Leu Ala Phe Ser Gly Val Met
385 390 395 400
Val Ser Met Lys Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Lys Glu Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asp Met
450 455 460
Ala Leu
465
<210> 66
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 66
Met Leu Ser Ser Thr Ile Gln Thr Leu Thr Leu Val Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Leu Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Gln Thr Glu Ile Thr Ala Pro
35 40 45
Ile Met Pro Leu Asp Cys Ala Asn Ala Pro Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Ala Lys Gly Val Thr Leu Leu Leu Pro Gly Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Gln Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ile Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Thr Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Asn
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Thr Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Ser Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Lys Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Ile Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Gln Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Ile Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Leu Ala Ser Asn Glu Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Ser Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Ile Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Glu
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asp Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asn Gly Asp Lys Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Lys Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Glu Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Arg
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Val Glu Met Val His Asp
420 425 430
Gly Gly Lys Thr Thr Trp His Ser Ala Ala Thr Ala Val Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Thr Leu
465
<210> 67
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 67
Met Leu Pro Ser Thr Ile Gln Met Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Ile Tyr Val Ser Ala Ser Leu Ser Tyr Leu Ile
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Arg Thr Glu Ile Thr Ala Pro
35 40 45
Ile Met Ser Leu Asp Cys Ala Asn Ala Ser Asn Val His Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Phe Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Ser Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ala Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Val Arg Glu Pro Phe Ile Ala Cys Gly Pro Thr Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Ser
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Glu
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Thr Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ser Cys His Asp Gly Lys Glu Trp Thr Tyr Val Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Lys Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Arg Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Leu Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Glu
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Asn Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Asn Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Ser Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Thr Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Glu Lys Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Glu Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Gln Thr Glu Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Ala Asp Ser Gly Ala Leu Ala Phe Ser Gly Val Met
385 390 395 400
Ile Ser Met Lys Glu Pro Gly Trp Tyr Thr Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Arg Glu Thr Trp His Ser Ala Ala Thr Thr Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Lys Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Ala Leu
465
<210> 68
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 68
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Arg Thr Glu Val Thr Ala Pro
35 40 45
Ile Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Pro Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Ser Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Lys Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Ile Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Ser Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asn Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Gln Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Glu Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Lys Thr Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Thr Leu
465
<210> 69
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 69
Met Pro Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Ile Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Leu Asp Ile Leu Leu Lys Phe Pro Ser Thr Glu Ile Thr Ala Pro
35 40 45
Thr Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
His Ser Ala Thr Lys Gly Val Thr Leu Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Arg Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Met Arg Glu Pro Phe Ile Ala Cys Gly Pro Asn Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Thr Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Asn Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Lys Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Val Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Lys Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Ser Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Met Lys Glu Ile Phe Pro Thr Gly Arg Thr Gln
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Ser Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Glu
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asp Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asp Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Glu Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Glu Arg Lys Gly Met Gly Leu Tyr Val Lys Tyr Asn
370 375 380
Gly Asp Pro Trp Ala Asp Ser Asp Ala Leu Ala Phe Ser Gly Val Met
385 390 395 400
Val Ser Met Lys Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Lys Glu Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asp Met
450 455 460
Ala Leu
465
<210> 70
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 70
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Leu Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Arg Thr Glu Val Thr Ala Pro
35 40 45
Ile Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Val Thr Lys Gly Val Thr Pro Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Met Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ile Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Thr Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Lys Leu Arg His Leu Val Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Met Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Thr Tyr Thr Asp Thr Tyr His Ser Tyr Ala Lys Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Val Lys Glu Ile Phe Pro Thr Gly Arg Val Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Asn Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Arg Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Met Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Asn Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asn Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Ala Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Lys Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Glu Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Ile Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Arg
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Val Glu Met Val His Asp
420 425 430
Gly Gly Lys Lys Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Pro Leu Trp Asp Thr Val Thr Gly Val Asn Ile
450 455 460
Thr Leu
465
<210> 71
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 71
Met Leu Pro Thr Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Met Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Met Leu Leu Lys Phe Ser Arg Thr Glu Val Thr Ala Pro
35 40 45
Met Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Pro Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Gln Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Arg Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Val Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Val Pro Thr Val Glu Asn Ser Met Phe His Met Thr Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Ile Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Ser Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Arg Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Ile Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Glu
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Asn Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Lys Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Lys Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Gln Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Asp Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Val Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Met Glu Met Val His Asp
420 425 430
Gly Gly Lys Thr Thr Trp His Ser Ala Ala Thr Ala Val Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Thr Leu
465
<210> 72
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 72
Met Leu Pro Ser Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Ile Leu Leu Lys Phe Ser Arg Thr Glu Val Thr Ala Pro
35 40 45
Ile Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Pro Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Ile Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Lys Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Ile Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Ser Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Lys Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Ile Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Ile Lys Glu Ile Phe Pro Thr Gly Arg Val Lys
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Ser Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asn Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Gln Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Glu Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Ile Glu Met Val His Asp
420 425 430
Gly Gly Lys Thr Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Leu Leu Trp Asp Thr Val Thr Gly Val Asn Met
450 455 460
Thr Leu
465
<210> 73
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 73
Met Leu Ser Ser Thr Ile Gln Thr Leu Thr Leu Leu Leu Thr Ser Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Val Ser Ala Leu Leu Ser Tyr Leu Leu
20 25 30
Tyr Ser Asp Met Leu Leu Lys Phe Ser Arg Thr Glu Val Thr Ala Pro
35 40 45
Met Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Val Gln Ala Val Asn
50 55 60
Arg Ser Val Thr Lys Gly Val Thr Pro Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Met Tyr Pro Arg Leu Ser Cys Pro Gly Ser Thr Phe Gln Arg Ala Leu
85 90 95
Leu Ile Ser Pro His Arg Phe Gly Glu Ile Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Ile Arg Glu Pro Phe Val Ala Cys Gly Pro Lys Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Thr Ala Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Gly Asp Arg Asn Lys Leu Arg His Leu Val Ser Val Lys
145 150 155 160
Leu Gly Lys Ile Pro Thr Val Glu Asn Ser Met Phe His Met Ala Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Lys Glu Trp Thr Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Ile Lys Tyr Gly Glu
195 200 205
Thr Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Gly Gly Asn Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Ser Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Val Lys Glu Ile Phe Pro Thr Gly Arg Thr Gln
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Asn Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Asn Arg Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Ile Asp Thr Ala Glu Ile Arg Leu Met Cys Thr Glu
305 310 315 320
Thr Tyr Leu Asp Thr Pro Arg Pro Asp Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Lys Glu Gly Ser Gly Gly Ile Lys Gly Gly Phe
340 345 350
Val His Gln Arg Met Glu Ser Lys Ile Gly Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Lys Arg Met Gly Met Gly Leu Tyr Val Lys Tyr Asn
370 375 380
Gly Asp Pro Trp Ala Asp Ser Asp Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Ile Ser Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Arg
405 410 415
Asp Lys Lys Cys Asp Val Pro Cys Ile Gly Val Glu Met Val His Asp
420 425 430
Gly Gly Lys Glu Thr Trp His Ser Ala Ala Thr Ala Val Tyr Cys Leu
435 440 445
Met Gly Ser Gly Gln Pro Leu Trp Asp Thr Val Thr Gly Val Asp Met
450 455 460
Ala Leu
465
<210> 74
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 74
Met Leu Pro Thr Thr Ile Gln Thr Leu Thr Leu Phe Leu Thr Thr Gly
1 5 10 15
Gly Val Leu Leu Ser Leu Tyr Met Ser Ala Ser Leu Ser Tyr Leu Leu
20 25 30
Tyr Leu Asp Ile Leu Leu Lys Phe Pro Ser Thr Glu Ile Thr Ala Pro
35 40 45
Thr Met Pro Leu Asp Cys Ala Asn Ala Ser Asn Ala Gln Ala Val Asn
50 55 60
Arg Ser Ala Thr Lys Gly Val Thr Leu Leu Leu Pro Glu Pro Glu Trp
65 70 75 80
Thr Tyr Pro Arg Leu Ser Cys Gln Gly Ser Thr Phe Gln Lys Ala Leu
85 90 95
Leu Ile Ser Ser His Arg Phe Gly Glu Thr Lys Gly Asn Ser Ala Pro
100 105 110
Leu Ile Met Arg Glu Pro Phe Ile Ala Cys Gly Pro Asn Glu Cys Lys
115 120 125
His Phe Ala Leu Thr His Tyr Ala Thr Gln Pro Gly Gly Tyr Tyr Asn
130 135 140
Gly Thr Arg Glu Asp Arg Asn Asn Leu Arg His Leu Ile Ser Val Lys
145 150 155 160
Leu Gly Lys Val Pro Thr Val Glu Asn Ser Met Phe His Met Thr Ala
165 170 175
Trp Ser Gly Ser Ala Cys His Asp Gly Arg Glu Trp Ile Tyr Ile Gly
180 185 190
Val Asp Gly Pro Asp Asn Asn Ala Leu Leu Lys Val Lys Tyr Gly Glu
195 200 205
Ala Tyr Thr Asp Thr Tyr His Ser Tyr Ala Asn Lys Ile Leu Arg Thr
210 215 220
Gln Glu Ser Ala Cys Asn Cys Ile Arg Gly Asp Cys Tyr Leu Met Ile
225 230 235 240
Thr Asp Gly Pro Ala Ser Gly Val Ser Glu Cys Arg Phe Leu Lys Ile
245 250 255
Arg Glu Gly Arg Ile Met Lys Glu Ile Phe Pro Thr Gly Arg Val Glu
260 265 270
His Thr Glu Glu Cys Thr Cys Gly Phe Ala Ser Asn Lys Thr Ile Glu
275 280 285
Cys Ala Cys Arg Asp Ile Pro Tyr Thr Ala Lys Arg Pro Phe Val Lys
290 295 300
Leu Asn Val Glu Thr Asp Thr Ala Glu Ile Lys Leu Met Cys Thr Lys
305 310 315 320
Thr Tyr Leu Asp Asn Pro Arg Pro Asn Asp Gly Ser Ile Thr Gly Pro
325 330 335
Cys Glu Ser Asp Gly Asp Glu Gly Ser Gly Gly Ile Lys Gly Gly Leu
340 345 350
Val His Gln Arg Met Ala Ser Lys Ile Ala Arg Trp Tyr Ser Arg Thr
355 360 365
Met Ser Lys Thr Glu Arg Lys Gly Met Gly Leu Tyr Val Lys Tyr Asp
370 375 380
Gly Asp Pro Trp Thr Asp Ser Asp Ala Leu Ala Leu Ser Gly Val Met
385 390 395 400
Val Pro Met Glu Glu Pro Gly Trp Tyr Ser Phe Gly Phe Glu Ile Lys
405 410 415
Asp Arg Lys Cys Asp Val Pro Cys Ile Gly Leu Glu Met Val His Asp
420 425 430
Gly Gly Lys Lys Thr Trp His Ser Ala Ala Thr Ala Ile Tyr Cys Leu
435 440 445
Met Gly Ser Gly Arg Leu Leu Trp Asp Thr Val Thr Gly Val Asn Ile
450 455 460
Thr Leu
465
<210> 75
<211> 548
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<220>
<221> 尚未归类的特征
<222> (180)..(181)
<223> Xaa 可以是任何天然存在的氨基酸
<400> 75
Met Met Lys Ala Ile Leu Val Leu Leu Thr Phe Thr Ala Ala Tyr Ala
1 5 10 15
Asp Thr Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp
20 25 30
Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu
35 40 45
Glu Asp His Asn Gly Lys Leu Cys Lys Leu Gly Ala Pro Leu Leu Gly
50 55 60
Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser
65 70 75 80
Leu Thr Ala Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn
85 90 95
Gly Thr Cys Tyr Pro Gly Asp Phe Asp Tyr Glu Glu Leu Arg Glu Gln
100 105 110
Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Ser
115 120 125
Ser Trp Pro Asn His Thr Gly Val Thr Ala Ala Cys His Asn Gly Ser
130 135 140
Phe Tyr Arg Asn Leu Ile Trp Leu Val Lys Lys Gly Lys Glu Asn Ser
145 150 155 160
Tyr Pro Lys Leu Ser Lys Ser Tyr Asn Asn Lys Lys Glu Val Leu Val
165 170 175
Leu Trp Gly Xaa Xaa Ile His His Pro Ser Thr Asp Gln Gln Leu Tyr
180 185 190
Gln Asn Ala Ala Tyr Val Phe Val Gly Ser Ser Tyr Ser Arg Lys Phe
195 200 205
Pro Glu Ile Ala Arg Pro Lys Val Arg Asp Gln Gly Arg Met Asn Tyr
210 215 220
Tyr Trp Thr Leu Glu Pro Gly Asp Thr Ile Thr Phe Glu Ala Thr Gly
225 230 235 240
Asn Leu Val Ala Pro Arg Tyr Ala Phe Ala Leu Arg Gly Gly Ser Gly
245 250 255
Ile Ile Ser Asp Ala Pro Val His Asp Cys Asp Thr Cys Gln Thr Pro
260 265 270
Gly Ala Ile Asn Thr Ser Leu Thr Ser Leu Pro Phe Gln Asn Val His
275 280 285
Pro Val Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu
290 295 300
Arg Met Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly
305 310 315 320
Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met
325 330 335
Ile Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly
340 345 350
Tyr Ala Ala Asp Gln Lys Ser Thr Gln Asn Ala Ile Asp Gly Ile Thr
355 360 365
Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala
370 375 380
Val Gly Lys Glu Phe Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys
385 390 395 400
Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu
405 410 415
Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn
420 425 430
Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn Ala
435 440 445
Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn
450 455 460
Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser
465 470 475 480
Glu Glu Ser Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu
485 490 495
Ser Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser
500 505 510
Leu Val Leu Leu Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser
515 520 525
Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile Lys Asn Arg Asp Met Arg
530 535 540
Lys Asn Thr Leu
545
<210> 76
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 76
Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asn Arg His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Leu Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Thr Asn Arg Gly Val Thr Ala Ala Cys Pro His Ala Gly Thr Asn Ser
145 150 155 160
Phe Tyr Arg Asn Leu Ile Trp Leu Val Lys Lys Glu Asn Ser Tyr Pro
165 170 175
Lys Ile Asn Lys Ser Tyr Ile Asn Asn Lys Glu Lys Glu Val Leu Val
180 185 190
Leu Trp Ala Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Lys Asn Ala Asp Ala Tyr Val Phe Val Gly Ser Ser Arg Tyr Ser
210 215 220
Arg Lys Phe Glu Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Ala Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Asp Gln Gly Asp Thr
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Ala Pro Arg Tyr Ala Phe
260 265 270
Ala Leu Lys Arg Asn Ser Gly Ser Gly Ile Ile Ile Ser Asp Thr Ser
275 280 285
Val His Asp Cys Asp Thr Thr Cys Gln Thr Pro Asn Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Val Thr Ile Gly Glu Cys
305 310 315 320
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Met Ala Thr Gly Leu Arg
325 330 335
Asn Ile Pro Ser Val Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Arg Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Ser His
405 410 415
Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Val Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asp Thr Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val
530 535 540
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Lys Ile Cys Ile
565
<210> 77
<211> 565
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 77
Met Lys Val Lys Leu Leu Ile Leu Leu Cys Thr Phe Thr Ala Thr Tyr
1 5 10 15
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Ala Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile
50 55 60
Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ile Ser Lys Lys Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Pro Asn Pro Glu Asn Gly Ala Cys Tyr Pro Gly Glu Phe
100 105 110
Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Lys Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr
130 135 140
Val Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Glu Arg Ser Phe
145 150 155 160
Tyr Arg Asn Leu Ile Trp Leu Thr Val Lys Asn Gly Leu Tyr Pro Asn
165 170 175
Leu Ser Lys Ser Tyr Glu Asn Asp Lys Gly Lys Glu Val Leu Ile Leu
180 185 190
Trp Gly Val His His Pro Ser Asn Ile Gly Asp Gln Arg Thr Leu Tyr
195 200 205
His Thr Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg
210 215 220
Arg Phe Thr Pro Glu Ile Thr Lys Arg Pro Lys Val Arg Asp Gln Glu
225 230 235 240
Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile
245 250 255
Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Phe Ala
260 265 270
Leu Ser Arg Gly Leu Gly Ser Gly Ile Ile Thr Ser Asn Ala Pro Met
275 280 285
Asp Glu Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser
290 295 300
Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro
305 310 315 320
Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn
325 330 335
Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Val Gly Phe
340 345 350
Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His
355 360 365
His Gln Asn Gly Gln Gly Ser Gly Tyr Ala Ala Asp Gln Glu Ser Thr
370 375 380
Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Ile Ile Glu
385 390 395 400
Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys Leu
405 410 415
Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Leu Leu
420 425 430
Asp Val Trp Thr Tyr Asn Ala Glu Met Leu Val Leu Leu Glu Asn Glu
435 440 445
Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Ser Leu Tyr Glu Lys
450 455 460
Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys
465 470 475 480
Phe Glu Phe Tyr His Lys Cys Asn Asn Glu Cys Met Glu Ser Val Arg
485 490 495
Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Tyr Glu Glu Ser Lys Leu Asn
500 505 510
Arg Glu Lys Ile Asp Gly Val Lys Leu Asp Ser Met Gly Val Tyr Arg
515 520 525
Ile Leu Ala Ile Tyr Ser Thr Ala Ala Ser Ser Leu Val Leu Leu Val
530 535 540
Ser Leu Gly Ala Ile Ser Phe Trp Val Cys Ser Asn Gly Ser Leu Gln
545 550 555 560
Cys Arg Ile Cys Ile
565
<210> 78
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 78
Met Glu Ala Lys Leu Phe Val Leu Phe Cys Ala Phe Thr Ala Leu Lys
1 5 10 15
Ala Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Gly Gly Ile
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Thr Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Ala Ser Ser Trp Pro Asn His Asp
130 135 140
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser
145 150 155 160
Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys
305 310 315 320
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg
325 330 335
Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Leu Glu Gly Gly Trp Thr Gly Met Val Asn Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser
370 375 380
Thr Gln Ile Ala Ile Asp Gly Ile Ser Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Ile Gln Phe Thr Ser Val Gly Lys Glu Phe Asn His
405 410 415
Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Phe His Asp Phe Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Lys Ser Gln Leu Arg Asn Asn Ala Lys Glu Ile Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asn Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr
515 520 525
Arg Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu
530 535 540
Val Ser Leu Gly Ala Val Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Val Cys Ile
565
<210> 79
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 79
Met Lys Ala Ile Leu Val Val Leu Leu Tyr Thr Phe Thr Thr Ala Asn
1 5 10 15
Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Leu Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe
100 105 110
Ile Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp
130 135 140
Thr Asn Arg Gly Val Thr Ala Ala Cys Pro His Ala Gly Thr Asn Ser
145 150 155 160
Phe Tyr Arg Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Leu Ser Lys Ser Tyr Ile Asn Asn Lys Glu Lys Glu Val Leu Val
180 185 190
Leu Trp Ala Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu
195 200 205
Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Ser Ser Arg Tyr Ser
210 215 220
Arg Lys Phe Glu Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Ala Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe
260 265 270
Ala Leu Lys Arg Asn Ser Gly Ser Gly Ile Ile Ile Ser Asp Thr Ser
275 280 285
Val His Asp Cys Asp Thr Thr Cys Gln Thr Pro Asn Gly Ala Ile Asn
290 295 300
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Val Thr Ile Gly Glu Cys
305 310 315 320
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Met Ala Thr Gly Leu Arg
325 330 335
Asn Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser
370 375 380
Thr Gln Asn Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Lys
405 410 415
Leu Glu Arg Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe
420 425 430
Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asn Asn Glu Cys Met Glu Ser Val
485 490 495
Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu
530 535 540
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Arg Ile Cys Ile
565
<210> 80
<211> 565
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 80
Met Lys Val Lys Leu Leu Ile Leu Leu Cys Thr Phe Thr Ala Thr Tyr
1 5 10 15
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr
20 25 30
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asn Arg His Asn Gly Lys Leu Cys Leu Leu Lys Gly Ile
50 55 60
Ala Pro Leu Gln Leu Gly Asn Cys Ser Val Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Ser Leu Ile Ser Lys Lys Ser Trp Ser Tyr Ile
85 90 95
Val Glu Thr Pro Asn Pro Glu Asn Gly Ala Cys Tyr Pro Gly Glu Phe
100 105 110
Ala Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Thr Val Ser Ser Phe
115 120 125
Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr
130 135 140
Val Thr Gly Val Ser Ala Ser Cys Ser His Asn Gly Glu Arg Ser Phe
145 150 155 160
Tyr Arg Asn Leu Ile Trp Leu Thr Val Lys Asn Gly Leu Tyr Pro Asn
165 170 175
Leu Ser Lys Ser Tyr Glu Asn Asp Lys Gly Lys Glu Val Leu Ile Leu
180 185 190
Trp Gly Val His His Pro Ser Asn Ile Gly Asp Gln Arg Thr Leu Tyr
195 200 205
His Thr Glu Asn Ala Tyr Val Ser Val Val Ser Ser His Tyr Ser Arg
210 215 220
Arg Phe Thr Pro Glu Ile Thr Lys Arg Pro Lys Val Arg Asp Gln Glu
225 230 235 240
Gly Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile
245 250 255
Ile Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Phe Ala
260 265 270
Leu Ser Arg Gly Leu Gly Ser Gly Ile Ile Thr Ser Asn Ala Pro Met
275 280 285
Asp Glu Cys Asp Ala Lys Cys Gln Thr Pro Gln Gly Ala Ile Asn Ser
290 295 300
Ser Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Glu Cys Pro
305 310 315 320
Lys Tyr Val Arg Ser Ala Lys Leu Arg Met Val Thr Gly Leu Arg Asn
325 330 335
Val Pro Ser Ile Gln Ser Arg Gly Leu Leu Gly Ala Ile Ala Gly Phe
340 345 350
Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His
355 360 365
His Gln Asn Gly Gln Gly Ser Gly Tyr Ala Ala Asp Gln Glu Ser Thr
370 375 380
Gln Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Ile Ile Glu
385 390 395 400
Lys Met Asn Ile Gln Phe Thr Ser Val Gly Lys Glu Phe Asn His Leu
405 410 415
Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu
420 425 430
Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn Glu
435 440 445
Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Ser Leu Tyr Glu Lys
450 455 460
Val Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Val Gly Asn Gly Cys
465 470 475 480
Phe Glu Phe Tyr His Lys Cys Asp Asp Thr Cys Met Glu Ser Val Lys
485 490 495
Asn Gly Thr Tyr Asn Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn
500 505 510
Arg Glu Lys Ile Asp Gly Val Lys Leu Asp Ser Met Gly Val Tyr Arg
515 520 525
Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val
530 535 540
Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln
545 550 555 560
Cys Arg Val Cys Ile
565
<210> 81
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 81
Met Glu Ala Lys Leu Phe Val Leu Phe Cys Ala Phe Thr Ala Leu Lys
1 5 10 15
Ala Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser Thr Glu Thr
20 25 30
Val Asp Thr Val Leu Glu Lys Asn Val Ala Val Thr His Ser Val Asn
35 40 45
Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Gly Gly Ile
50 55 60
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly
65 70 75 80
Asn Pro Glu Cys Glu Leu Leu Ile Ser Lys Glu Ser Trp Ser Tyr Ile
85 90 95
Val Glu Ala Pro Asn Ser Asp Asn Gly Ala Cys Tyr Pro Gly Gln Phe
100 105 110
Ala Asp Tyr Glu Glu Leu Arg Glu His Leu Ser Ser Val Ser Ser Phe
115 120 125
Glu Lys Phe Glu Ile Phe Pro Lys Ala Ser Ser Trp Pro Asn His Asp
130 135 140
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro Tyr Ala Gly Ala Asn Ser
145 150 155 160
Phe Tyr Arg Asn Leu Leu Trp Ile Val Lys Lys Gly Asn Ser Tyr Pro
165 170 175
Lys Ile Asn Lys Ser Tyr Ile Asn Asn Lys Gly Lys Glu Val Leu Val
180 185 190
Leu Trp Gly Ile His His Pro Pro Thr Ser Thr Asp Gln Gln Ser Leu
195 200 205
Tyr Lys Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser
210 215 220
Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln
225 230 235 240
Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Asp Gln Gly Asp Thr
245 250 255
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Ala Pro Arg Tyr Ala Phe
260 265 270
Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro
275 280 285
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn
290 295 300
Ser Asn Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Glu Cys
305 310 315 320
Pro Lys Tyr Val Arg Ser Thr Lys Leu Arg Met Val Thr Gly Leu Arg
325 330 335
Asn Ile Pro Ser Val Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly
340 345 350
Phe Leu Glu Gly Gly Trp Thr Gly Met Val Asn Gly Trp Tyr Gly Tyr
355 360 365
His His Gln Asn Asp Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser
370 375 380
Thr Gln Ile Ala Ile Asp Gly Ile Ser Asn Lys Val Asn Ser Val Ile
385 390 395 400
Glu Lys Met Asn Ile Gln Phe Thr Ala Val Gly Lys Glu Phe Ser His
405 410 415
Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Leu
420 425 430
Leu Asp Val Trp Thr Tyr Asn Ala Glu Met Leu Val Leu Leu Glu Asn
435 440 445
Glu Arg Thr Leu Asp Phe His Asp Phe Asn Val Lys Asn Leu Tyr Glu
450 455 460
Lys Val Lys Ser Gln Leu Arg Asn Asn Ala Lys Glu Ile Gly Asn Gly
465 470 475 480
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val
485 490 495
Arg Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Tyr Glu Glu Ser Lys Leu
500 505 510
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr
515 520 525
Gln Ile Leu Ala Ile Tyr Ser Thr Ala Ala Ser Ser Leu Val Leu Leu
530 535 540
Val Ser Leu Gly Ala Val Ser Phe Trp Met Cys Ser Asn Gly Ser Leu
545 550 555 560
Gln Cys Lys Ile Cys Ile
565
<210> 82
<211> 571
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 82
Met Lys Thr Ile Ile Ala Phe Ser Cys Ile Leu Cys Leu Ile Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Ser Asp Asn Asn Ser Met Ala Thr Leu Cys Leu
20 25 30
Gly His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asp
35 40 45
Asp Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser
50 55 60
Thr Gly Arg Ile Cys Asn Ser Pro Gly His Gln Ile Leu Asp Gly Lys
65 70 75 80
Asn Cys Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Asp
85 90 95
Phe Gln Asn Lys Trp Asp Leu Phe Val Glu Arg Ser Thr Ala Tyr Ser
100 105 110
Gly Asn Cys Tyr Pro Tyr Tyr Val Pro Asp Tyr Ala Ser Leu Arg Glu
115 120 125
Gln Leu Ser Leu Val Ala Ser Ser Gly Thr Leu Glu Phe Thr Gln Glu
130 135 140
Ser Phe Asn Trp Thr Gly Val Ala Gln Asp Gly Ser Ser Tyr Ala Cys
145 150 155 160
Arg Arg Gly Ser Val Asn Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu
165 170 175
Tyr Leu Asn Tyr Lys Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn
180 185 190
Asp Lys Phe Asp Lys Leu Tyr Ile Trp Gly Val His His Pro Gly Thr
195 200 205
Asp Lys Asp Gln Thr Asn Leu Tyr Val Gln Ala Ser Gly Arg Val Val
210 215 220
Ser Thr Lys Arg Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg
225 230 235 240
Pro Trp Val Arg Gly Val Ser Ser Ile Ile Ser Ile Tyr Trp Thr Ile
245 250 255
Val Lys Pro Gly Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile
260 265 270
Ala Pro Arg Gly Tyr Phe Lys Ile Gln Ser Gly Lys Ser Ser Gly Ile
275 280 285
Met Arg Ser Asp Ala Pro Ile Asp Cys Asn Ser Glu Cys Ile Thr Pro
290 295 300
Asn Gly Ser Ile Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile
305 310 315 320
Thr Tyr Gly Ala Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu
325 330 335
Ala Thr Gly Met Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe
340 345 350
Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp
355 360 365
Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala
370 375 380
Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asn Gln Ile Thr Gly Lys
385 390 395 400
Leu Asn Arg Val Ile Lys Lys Thr Asn Glu Lys Phe His Gln Ile Glu
405 410 415
Lys Glu Phe Ser Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr
420 425 430
Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu
435 440 445
Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met
450 455 460
Asn Lys Leu Phe Glu Arg Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu
465 470 475 480
Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala
485 490 495
Cys Ile Gly Ser Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg
500 505 510
Asp Glu Ala Leu Asn Asn Arg Phe Gln Ile Lys Gly Val Gln Leu Lys
515 520 525
Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys
530 535 540
Phe Leu Leu Cys Val Val Leu Leu Gly Phe Ile Ser Met Trp Ala Cys
545 550 555 560
Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile
565 570
<210> 83
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 83
Met Lys Thr Ile Ile Ala Phe Ser Cys Ile Leu Cys Leu Ile Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Ser Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Met Val Lys Thr Ile Thr Asp Asp
35 40 45
Gln Val Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Arg Ile Cys Asn Ser Pro His Gln Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Ile Asp Ser Leu Leu Gly Asp Pro His Cys Asp Asp Phe Gln
85 90 95
Asn Lys Glu Trp Asp Leu Phe Val Glu Arg Ser Lys Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Tyr Val Pro Asp Tyr Ala Thr Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Asn Leu Glu Phe Thr Gln Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Ala Gln Asp Gly Ser Ser Tyr Ala Cys Arg Arg Gly Ser Val
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Tyr Asn Leu Asn Tyr Lys
165 170 175
Tyr Pro Glu Gln Asn Val Thr Met Pro Asn Asn Asp Lys Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Thr
195 200 205
Asn Leu Tyr Val Gln Ala Ser Gly Arg Val Ile Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Ile Ile Pro Asn Ile Gly Ser Arg Pro Trp Val Arg
225 230 235 240
Gly Val Ser Ser Ile Ile Ser Ile His Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Asn Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
His Ile Asp Glu Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Ser Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Ile Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Ile Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asn Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Thr Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Lys Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Ile Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Ser Lys Leu Phe
450 455 460
Glu Arg Thr Arg Arg Gln Leu Arg Glu Asn Ala Glu Asp Ile Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ser Cys Met Glu Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Ile Tyr Arg Asn Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Gln Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Arg Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Val
565
<210> 84
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 84
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Asn Met Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Phe Ser Thr
50 55 60
Gly Glu Ile Cys Asn Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro Gln Cys Asp Gly Phe Gln
85 90 95
Asn Asn Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala His Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asn Gly Gly Ser Ser Ala Cys Lys Arg Gly Pro Asn
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Phe Lys
165 170 175
Tyr Pro Ala Leu Glu Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ala Thr Asp Lys Asp Gln Ile
195 200 205
Ser Leu Tyr Ala Gln Ala Ala Gly Arg Ile Ile Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Val Arg
225 230 235 240
Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Arg Pro Gly
245 250 255
Asp Ile Leu Leu Ile Lys Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Ala Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Lys Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Ile Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Lys Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Ile Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Ile Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Ile Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Ala Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Leu Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Glu Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Met Ser Cys Phe Leu Leu Cys
530 535 540
Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Lys Cys Asn Ile Cys Ile
565
<210> 85
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 85
Met Lys Ala Ile Ile Ala Phe Ser Cys Ile Leu Cys Gln Ile Ser Ala
1 5 10 15
Gln Lys Leu Pro Gly Ser Asp Asn Ser Met Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asp Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Thr Ser Lys
50 55 60
Gly Glu Ile Cys Ser Ser Pro His Gln Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Asp Phe Gln
85 90 95
Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Thr Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Tyr Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Ile
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Thr Gln Glu Ser Phe Asp Trp Thr
130 135 140
Gly Val Ala Gln Asn Gly Ser Ser Tyr Ala Cys Arg Arg Gly Ser Val
145 150 155 160
Lys Ser Phe Phe Ser Arg Leu Asn Trp Leu His Asn Leu Asn Tyr Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Asp Asn Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Ser Asp Gln Thr
195 200 205
Ser Leu Tyr Val Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro Trp Ile Arg
225 230 235 240
Gly Val Ser Ser Ile Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Ile Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Asn Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Arg Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Leu Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Ile Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His His Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Ile Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Asn Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Gly Val Glu Gly Arg Ile Gln Asp Leu Glu Arg Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Met Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Thr Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asn His Asp Val Tyr Arg Asp Glu Ala Val
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Gln Leu Lys Leu Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Thr Ser Cys Phe Leu Leu Cys
530 535 540
Ala Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 86
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 86
Met Lys Thr Ile Ile Ala Phe Ser Cys Ile Leu Cys Leu Ile Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Ser Asp Asn Ser Met Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asp Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Ser Ser Thr
50 55 60
Gly Arg Ile Cys Asn Ser Pro His Gln Ile Leu Asp Gly Lys Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Asp Phe Gln
85 90 95
Asn Lys Glu Trp Asp Leu Phe Val Glu Arg Ser Thr Ala Tyr Ser Asn
100 105 110
Cys Tyr Pro Tyr Tyr Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Thr Gln Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Ala Gln Asp Gly Ser Ser Tyr Ala Cys Arg Arg Gly Ser Val
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Tyr Asn Leu Asn Tyr Lys
165 170 175
Tyr Pro Glu Gln Asn Val Thr Met Pro Asn Asn Asp Lys Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Gly Thr Asp Lys Asp Gln Thr
195 200 205
Asn Leu Tyr Val Gln Ala Ser Gly Arg Val Ile Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Val Ile Pro Asn Ile Gly Ser Arg Pro Trp Val Arg
225 230 235 240
Gly Val Ser Ser Ile Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Ser Thr Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
His Ile Asp Glu Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Val Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Thr Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Lys Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Arg Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ala Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Val Tyr Arg Asp Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Gln Leu Lys Ser Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys
530 535 540
Val Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Ile
565
<210> 87
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 87
Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Leu Cys Leu Val Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Asn Met Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Ile Thr Asn Asp
35 40 45
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Ser Thr Ser Lys
50 55 60
Gly Glu Ile Cys Ser Ser Pro His Gln Ile Leu Asp Gly Glu Asn Cys
65 70 75 80
Thr Leu Ile Asp Ala Leu Leu Gly Asp Pro His Cys Asp Asp Phe Gln
85 90 95
Asn Lys Lys Trp Asp Leu Phe Val Glu Arg Ser Lys Ala His Ser Asn
100 105 110
Cys Tyr Pro Tyr Tyr Val Pro Asp Tyr Ala Thr Leu Arg Ser Leu Val
115 120 125
Ala Ser Ser Gly Asn Leu Glu Phe Thr Gln Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asp Gly Ala Ser Ser Ala Cys Lys Arg Arg Ser Ser
145 150 155 160
Lys Ser Phe Phe Ser Arg Leu Asn Trp Leu His Asn Leu Asn Tyr Lys
165 170 175
Tyr Pro Ala Leu Asn Val Thr Met Pro Asn Asn Asp Asn Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Lys Asp Gln Thr
195 200 205
Ser Leu Tyr Ile Gln Ala Ser Gly Arg Val Thr Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Ala Val Ile Pro Asn Ile Gly Ser Arg Pro Arg Val Arg
225 230 235 240
Asp Ile Pro Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Arg Pro Gly
245 250 255
Asp Ile Leu Leu Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Arg Ser Gly Lys Ser Ser Ile Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Asn Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Ser Asn Asp Lys Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Arg Tyr Ile Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Ile Pro Glu Lys Gln Thr Arg Gly Ile Phe Gly Ala Lys Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asn Gly Trp Tyr Gly
355 360 365
Phe Arg His Gln Asn Ser Glu Gly Arg Gly Gln Ala Ala Asp Leu Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys Leu Asn Arg Leu
385 390 395 400
Ile Gly Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser
405 410 415
Glu Ile Glu Gly Arg Ile Gln Asp Leu Glu Arg Tyr Val Glu Asp Thr
420 425 430
Lys Ile Asp Ile Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Met Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Ser Lys Leu Phe
450 455 460
Glu Arg Thr Arg Arg Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn
465 470 475 480
Gly Cys Leu Lys Ile Tyr His Lys Cys Asp Asn Ser Cys Met Glu Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asp His Asp Ile Tyr Arg Asn Glu Ala Leu
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Lys Ser Glu Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Thr Ser Cys Phe Leu Leu Cys
530 535 540
Ala Val Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Lys Gly Asn Ile
545 550 555 560
Lys Cys Asn Ile Cys Ile
565
<210> 88
<211> 566
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 88
Met Lys Ala Ile Ile Ala Phe Ser Cys Ile Leu Cys Gln Ile Phe Ala
1 5 10 15
Gln Lys Leu Pro Gly Asn Asp Asn Ser Thr Ala Thr Leu Cys Leu Gly
20 25 30
His His Ala Val Ser Asn Gly Thr Leu Val Lys Thr Ile Thr Asp Asp
35 40 45
Gln Val Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Phe Ser Met
50 55 60
Gly Lys Ile Cys Lys Asn Pro His Arg Ile Leu Asp Gly Ala Asn Cys
65 70 75 80
Thr Leu Ile Asp Ser Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln
85 90 95
Asn Glu Lys Trp Asp Leu Phe Ile Glu Arg Ser Lys Ala Phe Ser Asn
100 105 110
Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu Arg Ser Leu Ile
115 120 125
Ala Ser Ser Gly Thr Leu Glu Phe Asn Asn Glu Ser Phe Asn Trp Thr
130 135 140
Gly Val Thr Gln Asn Gly Gly Ser Ser Ala Cys Lys Arg Gly Pro Asn
145 150 155 160
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Thr His Leu Asn Phe Lys
165 170 175
Tyr Pro Ala Leu Glu Val Thr Met Pro Asn Asn Glu Gln Phe Asp Lys
180 185 190
Leu Tyr Ile Trp Gly Val His His Pro Ala Thr Asp Lys Asp Gln Ile
195 200 205
Ser Leu Tyr Ala Gln Ala Ala Gly Arg Ile Ile Val Ser Thr Lys Arg
210 215 220
Ser Gln Gln Thr Ile Ile Pro Asn Ile Gly Ser Arg Pro Trp Ile Arg
225 230 235 240
Gly Val Ser Ser Ile Ile Ser Ile His Trp Thr Ile Val Lys Pro Gly
245 250 255
Asp Met Leu Leu Ile Asn Ser Thr Gly Asn Ile Ile Ala Pro Arg Gly
260 265 270
Tyr Phe Lys Ile Gln Thr Gly Lys Ser Ser Val Met Arg Ser Asp Ala
275 280 285
Pro Ile Gly Lys Cys Asn Ser Ala Cys Ile Thr Pro Asn Gly Ser Ile
290 295 300
Pro Asn Asp Arg Pro Phe Gln Asn Val Asn Lys Ile Thr Tyr Gly Ala
305 310 315 320
Cys Pro Lys Tyr Val Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met
325 330 335
Arg Asn Ile Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala
340 345 350
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Ile Asp Gly Trp Tyr Gly
355 360 365
Phe Arg His His Asn Ser Glu Gly Thr Gly Gln Ala Ala Asp Ile Lys
370 375 380
Ser Thr Gln Ala Ala Ile Asn Gln Ile Asn Gly Lys Leu Asn Arg Val
385 390 395 400
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Leu
405 410 415
Glu Val Glu Gly Arg Ile Gln Asp Ile Glu Lys Tyr Ile Glu Asp Thr
420 425 430
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Ile Ala Leu Glu
435 440 445
Asn Gln His Thr Ile Asp Leu Ala Asp Ser Glu Met Asn Lys Leu Phe
450 455 460
Glu Lys Thr Lys Lys Gln Leu Arg Glu Asn Ala Glu Asp Ile Gly Asn
465 470 475 480
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Thr Cys Ile Gly Ser
485 490 495
Ile Arg Asn Gly Thr Tyr Asn His Asp Val Tyr Arg Asp Glu Ala Val
500 505 510
Asn Asn Arg Phe Gln Ile Lys Gly Val Gln Leu Lys Leu Gly Tyr Lys
515 520 525
Asp Trp Ile Leu Trp Ile Ser Phe Ala Met Ser Cys Phe Leu Leu Cys
530 535 540
Val Ala Leu Leu Gly Phe Ile Met Trp Ala Cys Gln Arg Gly Asn Ile
545 550 555 560
Arg Cys Asn Ile Cys Val
565
<210> 89
<211> 465
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 89
Met Asn Thr Asn Gln Arg Ile Ile Thr Ile Gly Thr Val Cys Met Ile
1 5 10 15
Val Gly Ile Ile Ser Leu Leu Leu Gln Ile Gly Asn Ile Val Ser Leu
20 25 30
Trp Ile Ser His Ser Ile Gln Thr Gly Asn Glu Asn His Thr His Glu
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Ile Ala Gln Val Thr Ser Val
65 70 75 80
Thr Ser Ile Ile Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Leu Glu Cys
115 120 125
Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His Ser
130 135 140
Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser Cys
145 150 155 160
Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser Val
165 170 175
Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr Ile
180 185 190
Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr Asn
195 200 205
Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Ile Leu Arg Thr
210 215 220
Gln Glu Ser Glu Cys Val Cys Asn Gly Ser Cys Phe Thr Val Met Thr
225 230 235 240
Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe Lys Glu Lys
245 250 255
Gly Lys Ile Ile Lys Ser Val Glu Leu Asp Ala Pro Asn Tyr His Tyr
260 265 270
Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val Val Cys Val Cys
275 280 285
Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val Ser Phe Asn Gln
290 295 300
Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly Val Phe Gly Asp
305 310 315 320
Asn Pro Arg Ser Asn Asp Gly Lys Gly Asn Cys Gly Pro Val Leu Ser
325 330 335
Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Arg Tyr Gly Asn Gly
340 345 350
Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg Ser Gly Phe Glu
355 360 365
Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp Ser Ser Phe Ser
370 375 380
Ile Lys Gln Asp Ile Ile Ala Leu Asn Asp Trp Ser Gly Tyr Ser Gly
385 390 395 400
Ser Phe Val Gln His Pro Glu Leu Thr Gly Met Asn Cys Ile Arg Pro
405 410 415
Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys Glu Asn Thr Ile
420 425 430
Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val Asn Ser Glu Thr
435 440 445
Ala Ser Trp Ser Trp Pro Asp Gly Ala Asp Leu Pro Phe Thr Ile Asp
450 455 460
Lys
465
<210> 90
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 90
Met Asn Thr Asn Gln Arg Ile Ile Thr Ile Gly Thr Val Cys Met Ile
1 5 10 15
Val Gly Ile Ile Ser Leu Leu Leu Gln Ile Gly Asn Ile Val Ser Leu
20 25 30
Trp Ile Ser His Ser Ile Gln Thr Gly Trp Glu Asn His Thr Glu Met
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Val Asn Asn Thr Trp Val Asn Arg
50 55 60
Thr Tyr Val Asn Ile Ser Asn Ile Lys Ile Ala Thr Ile Gln Asp Val
65 70 75 80
Thr Ser Ile Ile Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Val Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Met Arg Glu Pro Phe Ile Ser Cys Ser Gln Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro His Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Val Gly Glu Val Pro Ser Pro Tyr Asn Ser Lys Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asp Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Lys Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Leu Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Val Glu Lys Gly Lys Ile Ile Lys Ser Ile Glu Leu Asp Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Ser Asn Asp Gly Lys Gly Asn Cys Gly
325 330 335
Pro Val Leu Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Arg
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Asn Ser Arg
355 360 365
Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp
370 375 380
Ser Ser Phe Ser Met Lys Gln Asp Ile Ile Ala Leu Asn Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Met Asn
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys
420 425 430
Glu Ser Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Glu Thr Ala Ser Trp Ser Trp Pro Asp Gly Ala Asn Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 91
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 91
Met Asn Thr Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val
65 70 75 80
Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Thr Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Ser Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Val Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile
275 280 285
Thr Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asp Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly
325 330 335
Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Arg
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Asn Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Asn Phe Ser Ile Lys Gln Asp Ile Val Gly Ile Asn Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 92
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 92
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Cys Met Thr
1 5 10 15
Ile Gly Ile Ala Ser Leu Ile Leu Gln Ile Gly Asn Ile Val Ser Leu
20 25 30
Trp Val Ser His Ser Ile Gln Thr Arg Trp Glu Asn His Thr Glu Val
35 40 45
Cys Asn Gln Asn Val Ile Thr Tyr Val Asn Asn Thr Leu Val Asn Arg
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Ile Ala Thr Ile Gln Asp Val
65 70 75 80
Thr Ser Ile Met Leu Ala Gly Asn Ser Ser Leu Cys Pro Ile Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Arg His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Thr Ser Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Asp Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Lys Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Ile Glu Lys Gly Lys Val Val Lys Ser Val Glu Leu Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val
275 280 285
Val Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Leu Gly Asp Asn Pro Arg Pro Asn Asp Arg Thr Gly Ser Cys Gly
325 330 335
Pro Val Phe Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Thr Ser Ser Arg
355 360 365
Ser Gly Phe Glu Met Ile Trp Asp Pro Asp Gly Trp Thr Arg Thr Asp
370 375 380
Asp Lys Phe Ser Val Lys Gln Asp Ile Ile Ala Leu Thr Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Met Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys
420 425 430
Glu Asn Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asp Ser Glu Thr Ala Ser Trp Ser Trp Pro Asp Gly Ala Asp Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 93
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 93
Met Asn Thr Asn Gln Arg Ile Ile Thr Ile Gly Thr Val Cys Met Ile
1 5 10 15
Val Gly Ile Ile Ser Leu Leu Leu Gln Ile Gly Asn Ile Val Ser Leu
20 25 30
Trp Ile Ser His Ser Ile Gln Thr Gly Trp Glu Asn His Thr Glu Met
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Ile Lys Ile Ala Thr Ile Gln Asp Val
65 70 75 80
Thr Ser Ile Ile Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Gln Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Lys Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Leu Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Val Glu Lys Gly Lys Ile Ile Lys Ser Ile Glu Leu Asp Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val
275 280 285
Met Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Phe Gly Asp Asn Pro Arg Ser Asn Asp Gly Lys Gly Asn Cys Gly
325 330 335
Pro Val Leu Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Arg
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Asn Ser Arg
355 360 365
Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp
370 375 380
Ser Ser Phe Ser Met Lys Gln Asp Ile Ile Ala Leu Thr Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Met Asn
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys
420 425 430
Glu Ser Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Glu Thr Ala Ser Trp Ser Trp Pro Asp Gly Ala Asp Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 94
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 94
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr
1 5 10 15
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Asn Gln Ile Glu Thr
35 40 45
Cys Asn Gln Ser Val Ile Thr Tyr Val Asn Asn Thr Trp Val Asn Arg
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val
65 70 75 80
Val Ser Val Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Ile Ser Gly
85 90 95
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly
100 105 110
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ser Leu Leu Asn Asp Lys His
130 135 140
Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Thr
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Ser Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Asp Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr
225 230 235 240
Val Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile
275 280 285
Thr Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly
325 330 335
Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg
355 360 365
Asn Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp
370 375 380
Asn Asn Phe Ser Ile Lys Gln Asp Ile Ile Ala Leu Asn Asp Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys
420 425 430
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 95
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 95
Met Asn Thr Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Cys Met Thr
1 5 10 15
Ile Gly Ile Ala Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile
20 25 30
Trp Val Ser His Ser Ile Gln Thr Arg Trp Glu Asn His Thr Glu Val
35 40 45
Cys Asn Gln Asn Val Ile Thr Tyr Val Asn Asn Thr Trp Val Asn Gln
50 55 60
Thr Tyr Val Asn Ile Ser Asn Thr Asn Ile Ala Thr Ile Gln Asp Val
65 70 75 80
Thr Pro Ile Ile Leu Ala Gly Asn Ser Pro Leu Cys Pro Val Ser Gly
85 90 95
Trp Ala Val Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly
100 105 110
Asp Ile Phe Val Met Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu
115 120 125
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Arg His
130 135 140
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro His Arg Thr Leu Met Ser
145 150 155 160
Cys Pro Val Gly Glu Val Pro Ser Pro Tyr Asn Ser Lys Phe Glu Ser
165 170 175
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Thr Ser Trp Leu Thr
180 185 190
Ile Gly Ile Ser Gly Pro Asp Asp Gly Ala Val Ala Val Leu Lys Tyr
195 200 205
Asn Gly Val Ile Thr Asp Thr Ile Lys Ser Trp Arg Lys Asn Ile Leu
210 215 220
Arg Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Ser Cys Phe Thr
225 230 235 240
Ile Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe
245 250 255
Lys Ile Glu Lys Gly Lys Val Val Lys Ser Val Glu Leu Asn Ala Pro
260 265 270
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val
275 280 285
Val Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val
290 295 300
Ser Phe Asp Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly
305 310 315 320
Val Leu Gly Asp Asn Pro Arg Pro Asn Asp Arg Thr Gly Ser Cys Gly
325 330 335
Pro Val Phe Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Arg
340 345 350
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Thr Ser Ser Arg
355 360 365
Ser Gly Phe Glu Met Ile Trp Asp Pro Asp Gly Trp Thr Arg Thr Asp
370 375 380
Asp Lys Phe Ser Val Lys Gln Asp Ile Val Gly Ile Asn Glu Trp Ser
385 390 395 400
Gly Tyr Ser Gly Ser Phe Ile Gln His Pro Glu Leu Thr Gly Leu Asp
405 410 415
Cys Met Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys
420 425 430
Glu Asn Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val
435 440 445
Asp Ser Glu Thr Ala Ser Trp Ser Trp Pro Asp Gly Ala Asn Leu Pro
450 455 460
Phe Thr Ile Asp Lys
465
<210> 96
<211> 466
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 96
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Ile
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Asp Cys Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Pro Thr Ile Ile Glu Arg Asn Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Glu Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Ser Ser Ser Cys Leu
325 330 335
Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp Ala Phe
340 345 350
Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Glu Glu Leu
355 360 365
Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Gly Trp Ser Lys Pro Asn
370 375 380
Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Glu Arg Gly Asn Arg
385 390 395 400
Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser Cys Ile Asn
405 410 415
Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu Glu Thr Lys
420 425 430
Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly Thr Ser Gly
435 440 445
Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile Asn Leu Met
450 455 460
Pro Ile
465
<210> 97
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 97
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Asp Tyr Asn Ser Pro Pro Asn Asn
35 40 45
Gln Ala Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Thr Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Ser Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Arg Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp His Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asn Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Met
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Arg Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asn Asp Asn Asn Ala Thr Ala Ser Phe Ile Tyr Asp
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Lys Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Ile Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Glu Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Val Glu Glu Gly Lys Ile Val His Ile Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Phe Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asn Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Ser Asp Ser Val Ser Ser Ser Tyr
325 330 335
Cys Leu Asp Pro Asn Asn Glu Lys Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu
355 360 365
Thr Leu Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Ala Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Glu Lys
385 390 395 400
Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Ile Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Gln
420 425 430
Glu Thr Glu Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ala Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Ser Leu Met Pro Ile
465
<210> 98
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 98
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Ser Leu Thr
1 5 10 15
Leu Ala Ala Met Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Asn
20 25 30
Val Thr Leu His Phe Lys Gln His Asn Cys Asp Ser Ser Pro Asn Asn
35 40 45
His Val Met Phe Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Ile Thr Ile Glu Lys Glu Lys Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Lys Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Ala Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Arg His
130 135 140
Ser Asn Asp Thr Ile His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Ser Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys Tyr Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Ala Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Leu Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Arg Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asp Asp Lys Ser Ser Ser Ser Asn
325 330 335
Cys Leu Asn Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asp Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Leu Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Arg Gln
420 425 430
Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Ile Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ser Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Phe Met Pro Ile
465
<210> 99
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 99
Met Asn Pro Asn Gln Arg Ile Ile Thr Ile Gly Ser Val Ser Leu Ile
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Val Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Asp Cys Asn Ser Ser Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Lys Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Val Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Ala Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Val Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Ile Thr Arg Glu Pro Tyr Val Ser Cys Asp Leu Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly His Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Ile Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly His Asp Glu Asn Ala Thr Ala Ser Ile Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser Gly Ser Ala
260 265 270
Lys His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Val Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Thr Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Phe Ser Ser Ser His
325 330 335
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Leu Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Lys Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Gly Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu
420 425 430
Glu Thr Lys Val Trp Trp Thr Ser Asn Ser Ile Leu Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Leu Met Pro Ile
465
<210> 100
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 100
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Ile
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Asp Tyr Asn Ser Pro Pro Asn Asn
35 40 45
Gln Val Met Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Lys Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asp Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Phe Ser Ser Ser His
325 330 335
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu
355 360 365
Thr Leu Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Glu Lys
385 390 395 400
Gly Asp Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu
420 425 430
Glu Thr Lys Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Leu Met Pro Ile
465
<210> 101
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 101
Met Asn Pro Asn Gln Arg Ile Ile Thr Ile Gly Ser Val Ser Leu Thr
1 5 10 15
Ile Ala Thr Ile Cys Phe Leu Met Gln Val Ala Ile Leu Val Thr Thr
20 25 30
Val Thr Leu His Phe Lys Gln His Asp Cys Asn Ser Ser Pro Asn Asn
35 40 45
Gln Val Met Phe Cys Glu Pro Thr Ile Ile Glu Arg Asn Thr Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Thr Thr Val Glu Lys Glu Ile Cys Pro Lys
65 70 75 80
Pro Ala Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Lys Ile Thr Gly
85 90 95
Phe Ala Pro Phe Ser Arg Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly
100 105 110
Ala Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Leu Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Arg His
130 135 140
Ser Asn Asp Thr Ile His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Asn Glu Leu Gly Ile Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile
165 170 175
Ala Trp Ser Ser Ser Ser Cys Tyr Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Ile Thr Gly Asn Asp Asn Asn Ala Thr Ala Ser Phe Ile Tyr Asp
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Lys Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Ile Cys Thr Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe
245 250 255
Val Glu Glu Gly Lys Ile Val His Ile Ser Thr Leu Ser Gly Ser Ala
260 265 270
Lys His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg
275 280 285
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Val Val Asp
290 295 300
Ile Asn Val Lys Asn Tyr Ser Ile Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Ser Asp Ser Val Ser Ser Ser Tyr
325 330 335
Cys Leu Asp Pro Asn Asn Glu Lys Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asp Asp Val Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Ile Lys Gly Trp Ser
370 375 380
Lys Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Gln
420 425 430
Glu Thr Glu Val Trp Trp Thr Ser Asn Ser Ile Ile Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ser Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Asn Phe Met Pro Ile
465
<210> 102
<211> 469
<212> PRT
<213> 人工
<220>
<223> 合成产生的蛋白质
<400> 102
Met Asn Ser Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Ser Leu Thr
1 5 10 15
Leu Ala Ala Met Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Asn
20 25 30
Val Thr Leu His Phe Asn Gln Cys Glu Cys His Tyr Pro Pro Asn Asn
35 40 45
Gln Val Ile Leu Cys Glu Pro Thr Ile Ile Glu Arg Asn Ile Thr Glu
50 55 60
Ile Val Tyr Leu Thr Asn Ile Thr Ile Glu Lys Glu Lys Cys Pro Lys
65 70 75 80
Leu Ala Glu Tyr Arg Ser Trp Ser Lys Pro Gln Cys Asn Ile Ala Gly
85 90 95
Phe Ala Pro Phe Ser Lys Asp Asn Ser Val Arg Leu Ser Ala Gly Gly
100 105 110
Asp Ile Trp Ile Thr Arg Glu Pro Tyr Val Ser Cys Asp His Asp Lys
115 120 125
Cys Tyr Gln Phe Ala Leu Gly His Gly Thr Thr Leu Asn Asn Gly His
130 135 140
Ser Asn Asn Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met
145 150 155 160
Ser Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Met
165 170 175
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val
180 185 190
Cys Val Thr Gly His Asp Glu Asn Ala Thr Ala Ser Ile Ile Tyr Asn
195 200 205
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg
210 215 220
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Ala Val Val
225 230 235 240
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Arg Ile Leu Phe
245 250 255
Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Leu Gly Ser Ala
260 265 270
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Phe Pro Gly Val Arg
275 280 285
Cys Val Cys Arg Asp Asn Trp Arg Gly Ser Asn Arg Pro Val Val Asp
290 295 300
Ile Asn Val Lys Asp Tyr Ser Thr Val Ser Ser Tyr Val Cys Ser Gly
305 310 315 320
Leu Val Gly Asp Thr Pro Arg Lys Asp Asp Lys Ser Ser Ser Ser Asn
325 330 335
Cys Leu Asn Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp
340 345 350
Ala Phe Asp Asp Gly Asn Asp Leu Trp Met Gly Arg Thr Ile Ser Glu
355 360 365
Lys Leu Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Glu Gly Trp Ser
370 375 380
Lys Ala Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Gly Arg
385 390 395 400
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Ile Glu Gly Lys Asn
405 410 415
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Arg Gln
420 425 430
Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Leu Val Phe Cys Gly
435 440 445
Thr Ser Gly Thr Tyr Gly Ala Gly Ser Trp Pro Asp Gly Ala Asp Ile
450 455 460
Ser Leu Met Pro Ile
465
<210> 103
<211> 1680
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 103
atgaaggtga agctgctggt gctgctgtgc acctttacag ccacctacgc cgacacaatc 60
tgtatcggct atcacgccaa caattccaca gacaccgtgg ataccgtgct ggagaagaac 120
gtgacagtga cccactccgt gaacctgctg gaggattctc acaatggcaa gctgtgcctg 180
ctgaagggca tcgccccact gcagctgggc aactgctctg tggccggctg gatcctgggc 240
aatcccgagt gtgagctgct gatcagcaag gagtcttgga gctacatcgt ggagacacca 300
aaccccgaga atggcacctg ctaccccggc tttgccgact atgaggagct gagggagcag 360
ctgagcagcg tgagcagctt cgagcggttc gagatcttcc ctaaggagtc ctcttggcca 420
aaccacacag tgaccaaggg cgtgtccgcc tcttgtagcc acaacggcag ctccttctac 480
cggaatctgc tgtggctgac cggcaagaac ggcctgtacc ctaatctgtc caagtcttat 540
gccaacaata aggagaagga ggtgctggtg ctgtggggag tgcaccaccc acctaacatc 600
ggcgatcagc tgtaccacac agagaatgcc tatgtgagcg tggtgtctag ccactactcc 660
cggtttaccc ccgagatcgc caagaggcct aaggtgcgcg accaggaggg cagaatcaac 720
tactattgga cactgctgga gcccggcgat accatcatct tcgaggccaa cggcaatctg 780
atcgccccta gatatgcctt tgccctgtcc aggggattcg gaagcggaat catctccaat 840
gcccctatgg acgagtgcga tgccaagtgt cagacaccac agggcgccat caactcctct 900
ctgccatttc agaatgtgca ccccgtgaca atcggcgagt gtcctaagta cgtgcggagc 960
gccaagctga gaatggtgac cggcctgcgg aacatcccaa gcatccagtc cagaggcctg 1020
tttggagcaa tcgcaggctt catcgaggga ggatggaccg gaatggtgga cggctggtac 1080
ggctatcacc accagaatga gcagggctct ggctatgccg ccgatcagaa gagcacacag 1140
aacgccatca atggcatcac caacaaggtc aattccgtga tcgagaagat gaacacacag 1200
tttaccgccg tgggcaagga gttcaataag ctggagcgga gaatggagaa cctgaataag 1260
aaggtggacg atggctttct ggacatctgg acatacaacg ccgagctgct ggtgctgctg 1320
gagaatgaga ggaccctgga cttccacgat tccaacgtga agaatctgta tgagaaggtg 1380
aagtctcagc tgaagaacaa tgccaaggag atcggcaacg gctgcttcga gttttaccac 1440
aagtgcaacg acgagtgtat ggagagcgtg aagaatggca catacgatta tcccaagtat 1500
tctgaggaga gcaagctgaa tagggagaag atcgatggcg tgaagctgga gagcatgggc 1560
gtgtaccaga tcctggccat ctattccacc gtggcaagct ccctggtgct gctggtgtct 1620
ctgggcgcca tcagcttctg gatgtgctcc aacggctctc tgcagtgccg catctgtatc 1680
<210> 104
<211> 1695
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 104
atgaaggtga agctgctggt gctgctgtgc accttcacag ccacctacgc cgacacaatc 60
tgtatcggct atcacgccaa caatagcaca gacaccgtgg ataccgtgct ggagaagaac 120
gtgacagtga cccactctgt gaatctgctg gagaacagcc acaatggcaa gctgtgcctg 180
ctgaagggca tcgccccact gcagctggga aactgcagcg tggcaggatg gatcctggga 240
aatccagagt gtgagctgct gatctccaag gagtcttgga gctacatcgt ggagaagcca 300
aaccccgaga atggcacatg ctaccccggc cacttcgccg actatgagga gctgagggag 360
cagctgagct ccgtgtctag cctggagcgg ttcgagatct ttcctaagga gtcctcttgg 420
ccaaaccaca cagtgaccgg cgtgtccgcc tcttgtagcc acaacggcga gagctccttt 480
tacaggaatc tgctgtggct gaccggcaag aacggcctgt accctaatct gtccaagtct 540
tatgccaaca ataaggagaa ggaggtgctg gtgctgtggg gagtgcacca cccacctaac 600
atcggcgatc agcgcgccct gtaccacaca gagaatgcct acgtgagcgt ggtgtctagc 660
cactactctc ggaagttcac ccccgagatc gccaagaggc ctaaggtgcg cgaccaggag 720
ggcagaatca actactattg gacactgctg gagcccggcg ataccatcat ctttgaggcc 780
aacggcaatc tgatcgcccc tagatatgcc ttcgccctga gcaggggatt tggatccggc 840
atcatcaact ctaatgcccc tatggacaag tgcgatgcca agtgtcagac cccacagggc 900
gccatcaact cctctctgcc attccagaat gtgcaccccg tgacaatcgg cgagtgtcct 960
aagtacgtgc ggagcgccaa gctgagaatg gtgaccggcc tgcggaacat cccaagcgtg 1020
cagtccagag gactgttcgg agcaatcgca ggctttatcg agggaggctg gacaggcatg 1080
atcgacggct ggtacggcta tcaccaccag aatgagcagg gcagcggcta tgccgccgat 1140
cagaagtcca cacagaacgc catcaatggc atcaccaaca aggtcaattc cgtgatcgag 1200
aagatgaaca tccagttcac cgccgtgggc aaggagttta ataagctgga gcggagaatg 1260
gagaacctga ataagaaggt ggacgatggc ttcatcgata tctggacata caacgccgag 1320
ctgctggtgc tgctggagaa tgagcggacc ctggactttc acgattctaa cgtgaagaat 1380
ctgtatgaga aggtgaagag ccagctgaag aacaatgcca aggaagtggg caacggctgc 1440
ttcgagtttt accacaagtg caacgacgag tgtatggagt ccgtgaagaa tgagacctac 1500
gattatccca agtattctga ggagagcaag ctgaataggg agaagatcga cggcgtgaag 1560
ctggattcca tgggcgtgta ccagatcctg acaatctatt ctaccgtggc cagctccctg 1620
gtgctgctgg tgagcctggg agccatctcc ttttggatgt gctccaacgg ctctctgcag 1680
tgcaagatct gtatc 1695
<210> 105
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 105
atgaaggcca tcctggtggt gctgctgtac acctttgcca cagccaacgc cgacaccctg 60
tgcatcggct atcacgccaa caatagcacc gacacagtgg atacagtgct ggagaagaat 120
gtgaccgtga cacactccgt gaacctgctg gaggataagc acaatggcaa gctgtgcaag 180
ctgaggggag tggcacctct gcacctgggc aagtgcaaca tcgccggctg gatcctgggc 240
aatccagagt gtgagtccct gtctaccgcc agctcctggt cctacatcgt ggagacctct 300
agctccgaca acggcacatg ctaccctggc gactttatcg attatgagga gctgagggag 360
cagctgtcta gcgtgagcag cttcgagcgg ttcgagatct tccccaagac cagctcctgg 420
cctaaccacg atagcaataa gggagtgaca gcagcatgtc cacacgcagg cgccaagtcc 480
ttctacaaga acctgatctg gctggtgaag aagggcaatt cttaccccaa gctgagcaag 540
tcctatatca acgacaaggg caaggaggtg ctggtgctgt ggggcatcca ccacccatct 600
accagcgccg accagcagtc cctgtaccag aatgccgatg cctacgtgtt cgtgggcaca 660
tcccggtact ctaagaagtt caagccagag atcgccatcc ggcccaaggt gagagaccag 720
gagggcagaa tgaactacta ttggaccctg gtggagccag gcgataagat cacctttgag 780
gcaacaggaa acctggtggt gcccagatat gccttcgcca tggagagaaa tgccggctcc 840
ggcatcatca tctctgacac ccccgtgcac gattgcaaca ccacatgtca gacccctaag 900
ggcgccatca acacatctct gcctttccag aatatccacc caatcacaat cggcaagtgc 960
cccaagtacg tgaagagcac caagctgagg ctggccacag gcctgcgcaa tgtgcctagc 1020
atccagtcca ggggactgtt tggagcaatc gcaggcttca tcgagggagg atggaccgga 1080
atggtggatg gatggtacgg ctatcaccac cagaacgagc agggctctgg ctatgccgcc 1140
gacctgaaga gcacccagaa tgccatcgat aagatcacaa acaaggtcaa tagcgtgatc 1200
gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaatcacct ggagaagcgc 1260
atcgagaacc tgaataagaa ggtggacgat ggctttctgg acatctggac ctacaacgcc 1320
gagctgctgg tgctgctgga gaatgagagg acactggact accacgattc caacgtgaag 1380
aatctgtatg agaaggtgcg ctctcagctg aagaacaatg ccaaggagat cggcaacggc 1440
tgcttcgagt tttaccacaa gtgcgacaac acctgtatgg agtctgtgaa gaatggcaca 1500
tacgattatc ccaagtatag cgaggaggcc aagctgaatc gggaggagat cgatggcgtg 1560
aagctggaga gcaccagaat ctaccagatc ctggccatct attccacagt ggcctctagc 1620
ctggtgctgg tggtgagcct gggagcaatc tccttctgga tgtgctctaa cggcagcctg 1680
cagtgcagga tctgtatc 1698
<210> 106
<211> 1689
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 106
atgaaggtga agctgctggt gctgctgtgc accttcacag ccacctacgc cgacacaatc 60
tgtatcggct atcacgccaa caatagcaca gacaccgtgg ataccatcct ggagaagaac 120
gtgacagtga cccactctgt gaatctgctg gagaacagcc acaatggcaa gctgtgcctg 180
ctgaagggca tcgccccact gcagctggga aactgctccg tggcaggatg gatcctggga 240
aatccagagt gtgagctgct gatctctaag gagtcttgga gctacatcgt ggagaagcca 300
aaccccgaga atggcacatg ctaccccggc gacttcatca actatgagga gctgagggag 360
cagctgagct ccgtgtctag cctggagcgg ttcgagatct ttcctaagga gtcctcttgg 420
ccaaatcaca cagtgaccgg cgtgtccgcc tcttgtagcc acaacggcga gagctccttt 480
taccggaatc tgctgtggct gaccggcaag aacggcctgt accctaatct gtccaagtct 540
tatgccaaca ataaggagaa ggaggtgctg gtgctgtggg gagtgcacca cccacctaac 600
atcggcgatc agagagccct gtaccacaca gagaatgcct acgtgagcgt ggtgtctagc 660
cactactcta ggaagttcac ccccgagatc gccaagaggc ctaaggtgcg cgaccaggag 720
ggacgcatca actactattg gacactgctg gagcccggcg ataccatcat ctttgaggcc 780
aacggcaatc tgatcgcccc tagatatgcc ttcgccctga gcaggggatt tggatccggc 840
atcatcaact ctaatgcccc tatggacaag tgcgatgcca agtgtcagac cccacagggc 900
gccatcaact cctctctgcc attccagaat gtgcaccccg tgacaatcgg cgagtgtcct 960
aagtacgtgc ggagcgccaa gctgagaatg gtgaccggcc tgcggaacat cccaagcatc 1020
cagtccagag gactgctggg agcaatcgca ggctttatcg agggaggctg gacaggcatg 1080
atcgacggct ggtacggcta tcaccaccag aacgcacagg gaagcggata tgcagcagac 1140
cagaagtcca cacagaatgc catcgatgag atcaccaaca aggtcaattc cgtgatcgag 1200
aagatgaaca tccagttcac cgccgtgggc aaggagttta ataagctgga gcggagaatg 1260
gagaacctga ataagaaggt ggacgatggc ttcatcgata tctggacata caacgccgag 1320
ctgctgatcc tgctggagaa tgagcggacc ctggactttc acgatagcaa cgtgaagaat 1380
ctgtatgaga aggtgagaaa ccagctgaag aacaatgcca aggaagtggg caatggctgc 1440
ttcgagtttt accacaagtg caacgacgag tgtatggagt ccgtgaagaa tggcatctac 1500
gattatccca agtattctga ggagagcaag ctgaacaggg agaagatcga tggcgtgaag 1560
ctggagtcta tgggcgtgta ccagatcctg gccatctata gcaccgtggc aagctccctg 1620
gtgctgctgg tgagcctggg agccatctcc ttttggatgt gctccggcca ctcttgcaga 1680
atctgtatc 1689
<210> 107
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 107
atggaggcca tcctggtggt gctgctgtac acctttacca cagccaacgc cgacacactg 60
tgcgtgggct atcacgccaa caatagcacc gacacagtgg ataccgtgct ggagcggaat 120
gtgaccgtga cacactccgt gaacctgctg gaggattctc acaatggcaa gctgtgcaga 180
ctgaagggca tcgcccctct gcagctgggc aagtgcaaca tcgccggctg gctgctgggc 240
aatccagagt gtgagtccct gttttctaag gagagctggt cctacatcgt ggagaccccc 300
aaccctgaga atggcacatg ctaccctggc cacttcgccg actatgagga gctgagggag 360
cagctgaaca gcgtgagcag cttcgagcgg ttcgagatgt tcccaaagac ctctagctgg 420
cccgaccacg attccaataa gggagtgacc acagcatgtc cacacgcagg agcaaagggc 480
ttttacaaga acctgatctg gctgacaaag aagggcaata gctaccccaa gctgtctcag 540
agctatatca acgataaggg caaggagatc ctggtgctgt ggggcatcca ccacccaagc 600
accacagccg accagcagtc cctgtaccag aatgccgata cctacgtgtt cgtgggcaca 660
agcaagtact ccaagaagtt caagcctgag atcgccaccc ggccaaaggt gagagaccag 720
gagcggagaa tgaactacta ttggaccctg atcgagccag gcgataagat cacatttgag 780
gccaacggca atctggtggt gccccggtat gccttcacca tggagagaaa cgccggctcc 840
ggcatcatca tctctgacac aagcgtgcac gattgcaata ccacatgtca gacccccgag 900
ggcgccatca acacatccct gcccttccac aatatccacc ctatcaccat cggcgagtgc 960
cccaagtacg tgaggtctac caagctgcgc atggtgacag gcctgaggaa cgtgccttcc 1020
gtgcagtcta ggggactgtt tggagcaatc gcaggcttca tcgagggagg atggaccgga 1080
atgatggacg gatggtacgg ctatcaccac cagaatggcc agggctctgg ctatgccgcc 1140
gatcagaaga gcacccagaa cgccatcaat ggcatcacaa acaaggtcaa ttccgtgatc 1200
gacaagatga acacccagtt tacagccgtg ggcaaggagt tcaataagct ggagaagagg 1260
atggagaacc tgaataagaa ggtggacgat ggctttatcg acgtgtggac ctacaacgcc 1320
gagctgctga tcctgctgga gaatgagcgc acactggact accacgattc caaggtgaag 1380
aacctgtatg agaaggtgaa gtctcagctg aagaacaatg ccaaggagac cggcaatggc 1440
tgcttcgagt tttaccacaa gtgcaacaat gagtgtatgg agtctgtgaa gaacggcaca 1500
tacgactatc ctcggtatag cgaggaggcc aagctgaata gagagaagat cgatggcgtg 1560
aagctggaga gcacccgggt gtaccagatc ctggccatct attccacagc cgcctcctct 1620
ctggtgctgg tggtgtctct gggcgagatc agcttctgga tgtgcagcaa cggctccctg 1680
aggtgccgca tctgtatc 1698
<210> 108
<211> 1692
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 108
atgaagacca tcatcgccct gtcttatatc ctgtgcctgg tgttcgccca gaagctgcca 60
ggcaatgaca acaatagcac cgccacactg tgcctgggac accacgccgt gcccaacggc 120
acaatcgtga agaccatcac aaatgatcag atcgaggtga ccaacgccac agagctggtg 180
cagagctcca ccggcgagat ctgcgacagc cctcaccaga tcctggatgg cgagaactgt 240
acactgatcg acgcactgct gggcgaccca cagtgcgatg gcttccagaa taagaagtgg 300
gatctgtttg tggagcggtc taaggcctac agcaactgtt acccctatga cgtgcctgat 360
tatgcctccc tgagatctct ggtggcctct agcggcaccc tggagttcaa caatgagtct 420
tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagatctaat 480
agcttcttta gcaggctgaa ttggctgact cacctgaact tcaagtaccc cgccctgaac 540
gtgacaatgc ctaacaatga gcagtttgac aagctgtata tctggggagt gcaccaccca 600
ggcaccgaca aggatcagat cttcctgtac gcacagtccg gaaggatcac cgtgtccaca 660
aagagatctc agcaggccgt gatccctaat atcggatcta ggccaaggag gatcccaagc 720
cgcatctcca tctattggac catcgtgaag cctggcgaca tcctgctgat caacagcaca 780
ggcaatctga tcgccccaag gggctacttc aagatccgct ctggcaagag ctccatcatg 840
cggagcgacg ccccaatcgg caagtgctcc gagtgtatca cccccaatgg ctctatccct 900
aacgataagc catttcagaa cgtgaatcgg atcacatacg gcgcctgtcc cagatatgtg 960
aagcagaccc tgaagctggc cacaggcatg aggaacgtgc ctgagaagca gaccagggga 1020
atcttcggag caatcgcagg ctttatcgag aatggctggg agggcatggt ggacggctgg 1080
tacggctttc ggcaccagaa cagcgaggga agaggacagg cagcagacct gaagtccacc 1140
caggcagcca tcgatcagat caacggcaag ctgaataggc tgatcggcaa gacaaatgag 1200
aagttccacc agatcgagaa ggagttttcc gaggtggagg gccgcatcca ggatctggag 1260
aagtacgtgg aggacaccaa gatcgatctg tggagctata atgccgagct gctggtggcc 1320
ctggagaacc agcacaccat cgacctgaca gattccgaga tgaacaagct gttcgagaag 1380
accaagaagc agctgcggga gaatgccgag gacatgggca acggctgctt taagatctat 1440
cacaagtgcg ataatgcctg tatcggctcc atcagaaacg gcacatacga ccacgacgtg 1500
tacagggacg aggccctgaa caatcgcttc cagatcaagg gcgtggagct gaagtctggc 1560
tacaaggatt ggatcctgtg gatcagcttc gccatctcct gctttctgct gtgcgtggcc 1620
ctgctgggct ttatcatgtg ggcctgccag aagggcaaca tccggtgtaa tatcagatgc 1680
aacatctgta tc 1692
<210> 109
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 109
atgaagacca tcatcgccct gtctcacatc ctgtgcctgg tgttcgccca gaagatccca 60
ggcaacgaca atagcaccgc cacactgtgc atcggacacc acgccgtgcc caacggcatg 120
atcgtgaaga ccatcacaaa tgatagaatc gaggtgacca acgccacaga gctggtgcag 180
aatagctcca tcggcgagat ctgcgacagc ccccaccaga tcctggatgg cgagaattgt 240
acactgatcg acgcactgct gggcgaccct cactgcgatg gctttcagaa cgagaagtgg 300
gatctgttcg tggagagatc taaggccttt agcaattgtt acccctatga cgtgcctgat 360
tacgcctccc tgaggtctct ggtggcctct agcggcaccc tggagttcaa gaatgagtcc 420
tttaactgga ccggcgtggc ccagaacggc acatcctctg cctgcatccg gagaagctcc 480
tctagcttct tttctaggct gaattggctg acccacctga actacacata tccagccctg 540
aatgtgacaa tgcccaacaa ggagcagttc gacaagctgt acatctgggg agtgcaccac 600
ccaggcaccg acaaggatca gatctttctg tatgcccagt cctctggccg catcaccgtg 660
tccacaaagc ggtctcagca gacagccatc ccaaatatcg gaagcaggcc aaggatcagg 720
gacatcccta gccgcatctc catctactgg accatcgtga agcccggcga cgtgctggtc 780
atcaactcca atggcaacct gatcgcccct cgcggctatt tcaagatcca gtccggcaag 840
agctccatca tgcggtctga cgcccccatc ggcaagtgca agagcgagtg tatcacacct 900
aatggctcta tcagcaacga taagccattt cagaatgtga acaagatcac ctacggcgcc 960
tgtcctagat atgtgaagca ctccaccctg aagctggcca caggcatgag aaacgtgcca 1020
gagaagcaga ccaggggact gttcggagca atcgcaggct ttatcgagaa cggctgggag 1080
ggcatgaagg acggctggta cggcttcaga caccagaata gcgagggaag gggacaggca 1140
gcagatctga agtccacaca ggccgccatc aatcagatca acggcaagct gaaccgcctg 1200
atcggcaaga ccaatgagaa gttccaccag atcgagaagg agtttagcga ggtggaggga 1260
agggtgcagg acctggagaa gtacgtggag gactctaaga tcgatctgtg gagctataac 1320
gccggcctgc tggtggccct ggagaatcag cacaccatcg acctgacaga ttccgagatg 1380
aacaagctgt tcgagagaac caggaagcag ctgagagaga atgccgagga catgggcaac 1440
ggctgtttta caatctacca caagtgcgat aacgcctgta tcggctctat caggaatggc 1500
acctacgacc acaacgtgta tcgcgatgag gccctgaata agcggttcca gatcaagggc 1560
atcgagctga agtccggcta taaggattgg atcctgtgga tcagcttcgc catctcctgc 1620
tttctgctgt gcgtggccct gctgggcttt atcatgtggg cctgccagaa gggcaatatc 1680
cggtgcaaca tctgtatc 1698
<210> 110
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 110
atgaagacca tcatcgccct gtcttatatc ctgtgcctgg tgttcgccca gaagctgcct 60
ggcaacgaca atagcaccgc cacactgtgc ctgggacacc acgccgtgcc aaatggcaca 120
atcgtgaaga ccatcacaaa cgatcagatc gaggtgacca atgccacaga gctggtgcag 180
aacagctcca tcggcgagat ctgcgactcc ccacaccaga tcctggatgg cgagaactgt 240
accctgatcg acgcactgct gggcgaccca cagtgcgatg gcttccagaa taagaagtgg 300
gatctgtttg tggagagatc taaggcctac agcaactgtt acccctatga cgtgcctgat 360
tatgcctccc tgaggtctct ggtggcctct agcggcaccc tggagttcaa caatgagagc 420
tttaattgga ccggcgtgac acagaacggc acatcctctg cctgcatccg gagaagcaac 480
aattccttct tttctagact gaactggctg actcacctga atttcaagta ccctgccctg 540
aatgtgacaa tgccaaacaa tgagcagttt gacaagctgt atatctgggg agtgcaccac 600
cctggcaccg acaaggatca gatcttcctg tacgcacaga gctccggaag gatcaccgtg 660
tccacaaagc ggtctcagca ggccgtgatc cctaacatcg gaagcaggcc aaggatcagg 720
gacatcccaa gccgcatctc catctattgg accatcgtga agccaggcga tatcctgctg 780
atcaactcca caggcaatct gatcgccccc agaggctact tcaagatcag gtctggcaag 840
tctagcatca tgcggagcga cgcccccatc ggcaagtgca agtccgagtg tatcacccct 900
aacggctcta tcccaaatga taagcccttt cagaacgtga atagaatcac atacggcgcc 960
tgtcccagat atgtgaagca gaataccctg aagctggcca caggcatgcg caacgtgcct 1020
gagaagcaga ccaggggaat cttcggagca atcgcaggct ttatcgagaa tggctgggag 1080
ggcatggtgg acggctggta cggctttaga caccagaaca gcgagggaag gggacaggca 1140
gcagacctga agtccaccca ggccgccatc gatcagatca acggcaagct gaatcgcctg 1200
atcggcaaga caaacgagaa gttccaccag atcgagaagg agttttccga ggtggagggc 1260
cggatccagg atctggagaa gtacgtggag gacaccaaga tcgatctgtg gtcctataat 1320
gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga ttctgagatg 1380
aacaagctgt tcgagaagac caagaagcag ctgagagaga acgccgagga catgggcaat 1440
ggctgtttta agatctatca caagtgcgat aatgcctgta tcggctctat caggaacggc 1500
acatacgacc acgacgtgta ccgcgacgag gccctgaaca atcggttcca gatcaagggc 1560
gtggagctga agagcggcta caaggattgg atcctgtgga tcagcttcgc catctcctgc 1620
tttctgctgt gcgtggccct gctgggcttt atcatgtggg cctgccagaa gggcaacatc 1680
aggtgcaata tctgtatc 1698
<210> 111
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 111
atgaagacca tcatcgccct gtcccacatc ctgtgcctgg tgttcgccca gaagatcccc 60
ggcaacgaca attccaccgc aatgctgtgc ctgggacacc acgccgtgtc taatggcaca 120
atcgtgaaga ccatcacaaa tgatcgcatc gaggtgacca acgccacaga gctggtgcag 180
agctcctcta ccggcgagat ctgcgacagc cctcaccgga tcctggatgg caagaactgt 240
acactgatcg acgcactgct gggcgaccca cactgcgatg gcttccagaa taaggagtgg 300
gatctgtttg tggagcggag caaggcccac tctaactgtt acccctatga cgtgcctgat 360
tacgtgagcc tgcggtccct ggtggcaagc tccggcaccc tggagttcaa gaatgagtct 420
tttaactgga ccggcgtggc ccagaacggc acatctagcg cctgcatccg gagatcctct 480
agctccttct ttagcagact gaattggctg acccacctga actacacata tccagccctg 540
aatgtgacaa tgcccaacaa tgagaagttc gacaagctgt acatctgggg agtgcaccac 600
ccaggcaccg acaacgatca gatctttctg tatgcacagg ccagcggaag gatcaccgtg 660
agcacaaagc ggtcccagca gacagtgatc ccaaatatcg gatccaggcc acgcgtgcgg 720
aacatccctt ctaggatcag catctactgg accatcgtga agcccggcga cgtgctggtc 780
atcaacagca atggcaacct gatcgcccct cgcggctatt tcaagatcca gagcggcaag 840
tctagcatca tgcggtccga cgcccctatc ggcaagtgca attccgagtg tatcacacct 900
aatggctcca tctctaacga taagccattt cagaatgtga acaagatcac ctacggcgcc 960
tgtccaagat atgtgaagca ctccaccctg aagctggcca caggcatgag gaacgtgccc 1020
gagagacaga caaggggcat cttcggcgcc atcgccgtgt ttatcgagaa tggctgggag 1080
ggcatgatgg acggctggta cggcttccgc caccagaact ctgagggcac cggacaggca 1140
gcagatctga agagcacaca ggccgccatc aatcagatca acggcaagct gaatagactg 1200
atcgagaaga ccaacgagaa gttccaccag atcgagaagg agttttctga ggtggagggc 1260
agggtgcagg acctggagaa gtacgtggag gactccaaga tcgatctgtg gtcttataat 1320
gcaggactgc tggtggccct ggagaaccag cacaccatcc acctgacaga tagcgagatg 1380
aacaagctgt tcgagagaac caagaagcag ctgagggaga atgccgagga catgggcaac 1440
ggctgtttta caatctacca caagtgcgat aatgcctgta tcgagtccat cagaaatggc 1500
acctacgacc acaacgtgta tagagatgag gccctgaaca agaggttcca gatcaagggc 1560
atcgagctga agtctggcta taaggactgg gtgctgtgga tctctttcgc catcagctgc 1620
tttctgctgt gcgtggtgct gctgggcttt atcatgtggg cctgccagcg cggcaatatc 1680
cggtgcaaca tctgtatc 1698
<210> 112
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 112
atgagaacca tcatcgccct gagctatatc ttctgtctgg tgtttgccca gaagctgcca 60
gagaacgaca attccaccgc cacactgtgc atcggacacc acgccgtgcc caacggcaca 120
ctggtgaaga ccatcacaaa cgatcaggtg gaggtgacca atgccacaga gctggtgcag 180
agctcctcta ccggaggaat ctgcgactcc ccacaccaga tcctggatgg caagaattgt 240
acactggtgg acgcactgct gggcgaccct cactgcgatg gcttccagaa cgagaagtgg 300
gatctgttcg tggagagatc taaggccttt agcaattgtt acccatatga cgtgcaggat 360
tacgcctctc tgaggagcct gatcgcaagc tccggcaccc tggagttcaa caatgagagc 420
tttaactggg ccggcgtgac ccagaatggc acatctagcg cctgcaagcg gagatctaac 480
tcctctttct ttagcaggct gaattggctg acccacctga agttcaagta cccagccctg 540
aacgtgacaa tgcccaataa ggagcagttt gacaagctgt atatctgggg agtgcaccac 600
ccatccaccg actctgatca gatctccctg tacgcccagg cctctggcag agtgaccgtg 660
tccacaaaga ggtctcagca gacagccatc ccaaacatcg gctccaggcc ccgcatccgg 720
aatatcccta gccgcgtgtc catctattgg accatcgtga agcccggcga tatcctgctg 780
atcaactgta caggcaatct gatcgcccct cgcggctact tcaagatccg gaacggcaag 840
agctccatca tgcggtccaa cgcccctatc ggcaagtgca atagcgcctg tatcacccca 900
aacggctcca tccccaatgg caagcctttt cagaacgtga atagagtgac atacggcgcc 960
tgccctagat atgtgaagca gtctaccctg aagctggcca caggcatgcg caacgtgcca 1020
gagaagcaga ccaggggact gttcggagca atcgcaggct ttatcgagaa cggctgggag 1080
ggcatgaagg acggctggta cggcttccgg caccagaatt ccgagggaat cggacaggca 1140
gcagacctga agtctacaca ggccgccgtg gatcagatca acggcaagct gaatcgcgtg 1200
atcgagaaga ccaacgagaa gttccaccag atcgagaagg agtttacaga ggtggagggc 1260
cggatccagg acatcgagaa gtacgtggag gacaccaagg tggatctgtg gtcttataac 1320
gccgagctgc tggtgagcct ggagaatcag cacaccatcg acatgacaga tagcgagatg 1380
aataagctgt tcgagagaac caggaagcag ctgagagaga acgccgagga tatcggcaat 1440
ggctgtttta agatctatca caagtgcaac aatgcctgta tcggctctat caggaacgag 1500
acatacgacc acaacgtgta ccgcgatgag gccctgaaca atcggttcca catcaagggc 1560
gtggagctga agagcggcta caagaactgg atcctgtgga tcagcttcgc cacctcctgc 1620
tttctgctgt gcatcgtgct gctgggcttt atcatgtggg cctgccagaa gggcaacatc 1680
aagtgcaata tctgtatc 1698
<210> 113
<211> 1713
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 113
atgaagacca tcatcgcctt ctcttgcatc ctgtgcctga tctttgccca gaagctgccc 60
ggctccgaca acaattctat ggccaccctg tgcctgggac accacgcagt gcctaatggc 120
acactggtga agaccatcac agacgatcag atcgaggtga ccaacgccac agagctggtg 180
cagagctcct ctaccggcag gatctgtaat tcccctggcc accagatcct ggatggcaag 240
aactgcacac tgatcgacgc cctgctgggc gatccacact gtgacgattt ccagaataag 300
tgggacctgt ttgtggagcg gagcaccgcc tactccggca actgctatcc ctactatgtg 360
cctgattacg ccagcctgag agagcagctg tccctggtgg ccagctccgg caccctggag 420
ttcacacagg agtcttttaa ttggaccgga gtggcacagg acggctctag ctacgcctgt 480
cggagaggca gcgtgaacaa ttctttcttt agccgcctga actggctgta tctgaattac 540
aagtatcccg ccctgaacgt gacaatgcct aacaatgaca agttcgataa gctgtacatc 600
tggggagtgc accacccagg aaccgacaag gatcagacaa atctgtatgt gcaggccagc 660
ggcagggtgg tgtctaccaa gcgcagccag cagacagtga tccctaacat cggctcccgg 720
ccatgggtga gaggcgtgtc ctctatcatc tctatctact ggaccatcgt gaagcctggc 780
gacatcctgc tgatcaacag cacaggcaat ctgatcgccc caaggggcta tttcaagatc 840
cagtccggca agagctccgg catcatgcgc tctgacgccc caatcgattg caacagcgag 900
tgtatcaccc ccaacggctc catcccaaat gataagccct ttcagaacgt gaataggatc 960
acatacggcg cctgcccacg ctatgtgaag cagaataccc tgaagctggc cacaggcatg 1020
cggaacgtgc ccgagaagca gaccagaggc atcttcggcg ccatcgccgg ctttatcgag 1080
aatggctggg agggcatggt ggacggctgg tacggcttcc ggcaccagaa ctctgaggga 1140
accggacagg cagcagatct gaagagcacc caggcagcca tcaaccagat cacaggcaag 1200
ctgaatagag tgatcaagaa gaccaacgag aagttccacc agatcgagaa ggagtttagc 1260
gaggtggagg gaaggatcca ggacctggag aagtacgtgg aggacaccaa gatcgatctg 1320
tggtcctata atgccgagct gctggtggcc ctggagaacc agcacaccat cgacctgaca 1380
gattctgaga tgaacaagct gttcgagagg accaggaagc agctgaggga gaacgcagag 1440
gacatgggca atggctgctt taagatctac cacaagtgcg ataatgcctg tatcggctcc 1500
atcagaaacg gcacatacga ccacgacgtg taccgggacg aggccctgaa caatagattc 1560
cagatcaagg gcgtgcagct gaagagcggc tataaggatt ggatcctgtg gatctccttc 1620
gccatctctt gctttctgct gtgcgtggtg ctgctgggct ttatctccat gtgggcctgt 1680
cagaagggca acatcagatg caatatctgt atc 1713
<210> 114
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 114
atgaagacca tcatcgcctt cagctgcatc ctgtgcctga tctttgccca gaagctgcct 60
ggctccgaca attctaccgc caccctgtgc ctgggacacc acgcagtgcc aaacggcaca 120
atggtgaaga ccatcacaga cgatcaggtg gaggtgacca atgccacaga gctggtgcag 180
agctcctcta ccggccggat ctgcaatagc ccacaccaga tcctggatgg caagaactgt 240
acactgatcg actccctgct gggcgatccc cactgcgacg atttccagaa taaggagtgg 300
gacctgtttg tggagaggtc taaggcctac agcaactgtt atccctacta tgtgcctgat 360
tacgccaccc tgcgctccct ggtggccagc tccggcaatc tggagttcac ccaggagtct 420
tttaactgga caggagtggc acaggacggc tctagctacg catgccggag aggcagcgtg 480
aactccttct tttctagact gaattggctg tataacctga attacaagta tcctgagcag 540
aacgtgacca tgccaaacaa tgacaagttc gataagctgt acatctgggg agtgcaccac 600
cctggaaccg acaaggatca gacaaatctg tatgtgcagg ccagcggcag agtgatcgtg 660
agcaccaagc ggagccagca gacaatcatc ccaaacatcg gctctcggcc ctgggtgaga 720
ggcgtgtcct ctatcatcag catccactgg accatcgtga agccaggcga catcctgctg 780
atcaactcca caggcaatct gatcgccccc agaggctact tcaagatcca gaatggcaag 840
agctccatca tgagatccga cgcccacatc gatgagtgca actctgagtg tatcaccccc 900
aacggctcta tcagcaatga taagcctttt cagaacgtga ataagatcac atacggcgcc 960
tgccccaggt atatcaagca gaataccctg aagctggcca caggcatgag gaacatccct 1020
gagaagcaga cccgcggcat cttcggagca atcgcaggct ttatcgagaa cggctgggag 1080
ggcatggtga atggctggta tggcttccgc caccagaaca gcgagggaac cggacaggca 1140
gcagacctga agtccaccca ggccgccatc aaccagatca caggcaagct gaatagagtg 1200
atcaagaaga ccaacgagaa gttccaccag atcgagaagg agttttctga ggtggagggc 1260
agaatccagg atctggagaa gtacgtggag gacaccaaga tcgatatctg gtcctataat 1320
gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgacaga tagcgagatg 1380
tccaagctgt tcgagcggac caggcgccag ctgagagaga acgccgagga catcggcaat 1440
ggctgtttta agatctacca caagtgcgat aatagctgta tggagtccat caggaacggc 1500
acatacgacc acgatatcta tcggaacgag gccctgaaca atagattcca gatcaagggc 1560
gtgcagctga agagcggcta taaggactgg atcctgtgga tctctttcgc catcagctgc 1620
tttctgctgt gcgtggtgct gctgggcttt atcatgtggg cctgccagag gggcaacatc 1680
cgctgcaata tctgcgtg 1698
<210> 115
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 115
atgaaggcca tcctggtggt gctgctgtac acctttacca cagccaacgc cgacacactg 60
tgcatcggct atcacgccaa caatagcacc gacacagtgg ataccgtgct ggagaagaat 120
gtgaccgtga cacacagcgt gaacctgctg gaggattccc acaatggcaa gctgtgcaag 180
ctgaggggag tggcaccact gcacctgggc aagtgcaaca tcgcaggatg gctgctggga 240
aatccagagt gtgagagcct gtccaccgcc agctcctggt cctacatcgt ggagacctct 300
aacagcgaca atggcacatg ctacccaggc gacttcatca actatgagga gctgagggag 360
cagctgagca gcgtgagcag cttcgagcgg ttcgagatct tccccaagac cagctcctgg 420
cctaaccacg acaccaatag gggagtgaca gcagcatgtc cacacgcagg aacaaactcc 480
ttctaccgca atctgatctg gctggtgaag aagggcaatt cttaccctaa gctgtccaag 540
tcttatatca acaataagga gaaggaggtg ctggtgctgt gggcaatcca ccaccccagc 600
acctccgccg accagcagag cctgtaccag aacgccgatg cctacgtgtt cgtgggctct 660
agcaggtact cccgcaagtt cgagccagag atcgcaacca ggccaaaggt gagagaccag 720
gccggcagga tgaattacta ttggacactg gtggagcccg gcgataagat cacctttgag 780
gccacaggca acctggtggt gcctcggtat gccttcgccc tgaagagaaa ttctggcagc 840
ggcatcatca tctccgatac ctctgtgcac gactgcgata ccacatgtca gacccctaac 900
ggcgccatca atacaagcct gcctttccag aacatccacc cagtgacaat cggcgagtgc 960
cctaagtacg tgaagtccac caagctgagg atggccacag gcctgcgcaa tatcccaagc 1020
atccagtccc gcggcctgtt tggagcaatc gcaggcttca tcgagggagg atggaccgga 1080
atgatcgacg gatggtacgg ctatcaccac cagaacgagc agggctctgg ctatgccgcc 1140
gacctgaaga gcacccagaa tgccatcgat ggcatcacaa acaaggtcaa tagcgtgatc 1200
gagaagatga acacccagtt tacagccgtg ggcaaggagt tcaataagct ggagcggaga 1260
atggagaacc tgaataagaa ggtggacgat ggctttctgg atatctggac ctacaacgcc 1320
gagctgctgg tgctgctgga gaatgagcgg acactggact accacgattc caacgtgaag 1380
aatctgtatg agaaggtgag atctcagctg aagaacaatg ccaaggagat cggcaacggc 1440
tgcttcgagt tttaccacaa gtgcaacaat gagtgtatgg agtctgtgaa gaacggcacc 1500
tacgactatc ccaagtatag cgaggaggcc aagctgaatc gggaggagat cgatggcgtg 1560
aagctggaga gcaccagaat ctaccagatc ctggccatct attccacagt ggcctcctct 1620
ctggtgctgc tggtgtccct gggcgccatc tctttctgga tgtgctctaa cggcagcctg 1680
cagtgccgga tctgtatc 1698
<210> 116
<211> 1695
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 116
atgaaggtga agctgctgat cctgctgtgc acctttacag ccacctacgc cgacacaatc 60
tgtatcggct atcacgccaa caattccaca gacaccgtgg ataccatcct ggagaagaac 120
gtgacagtga cccactctgt gaatctgctg gagaacaggc acaatggcaa gctgtgcctg 180
ctgaagggaa tcgcaccact gcagctggga aactgcagcg tggcaggatg gatcctgggc 240
aatcctgagt gtgagtctct gatcagcaag aagtcctggt cttacatcgt ggagacaccc 300
aaccctgaga atggcgcctg ctaccccggc gagtttgccg attatgagga gctgagggag 360
cagctgagca ccgtgagcag cttcgagcgg ttcgagatct tcccaaagga gtctagctgg 420
cccaaccaca cagtgaccgg cgtgagcgcc tcctgttctc acaacggcga gaggtccttc 480
taccgcaatc tgatctggct gaccgtgaag aacggcctgt accctaatct gagcaagtcc 540
tatgagaatg acaagggcaa ggaggtgctg atcctgtggg gcgtgcacca cccatccaac 600
atcggcgatc agcgcacact gtaccacacc gagaatgcct acgtgagcgt ggtgtcctct 660
cactacagcc ggagattcac acctgagatc accaagcggc caaaggtgag agaccaggag 720
ggccggatca actactattg gacactgctg gagcctggcg ataccatcat ctttgaggcc 780
aacggcaatc tgatcgcccc atggtatgcc ttcgccctga gcagaggcct gggatccggc 840
atcatcacat ctaatgcccc aatggacgag tgcgatgcca agtgtcagac cccacaggga 900
gccatcaaca gctccctgcc ctttcagaat gtgcaccctg tgacaatcgg cgagtgtcca 960
aagtacgtga ggagcgccaa gctgaggatg gtgaccggac tgaggaacgt gccctccatc 1020
cagtctagag gcctgctggg agcaatcgca ggcttcatcg agggaggatg gacaggaatg 1080
gtggacggat ggtacggcta tcaccaccag aatggacagg gatccggata tgcagcagat 1140
caggagtcta cacagaacgc catcaatggc atcaccaaca aggtcaatag catcatcgag 1200
aagatgaaca tccagtttac ctccgtgggc aaggagttca atcacctgga gaagaggatc 1260
gagaacctga ataagaaggt ggacgatggc tttctggacg tgtggacata caacgccgag 1320
ctgctgatcc tgctggagaa tgagcgcacc ctggacttcc acgattccaa cgtgaagtct 1380
ctgtatgaga aggtgaagag ccagctgaag aacaatgcca aggaagtggg caatggctgc 1440
ttcgagtttt accacaagtg cgacgataca tgtatggagt ccgtgaagaa cggcacctac 1500
aattatccta agtatagcga ggagtccaag ctgaaccggg agaagatcga cggcgtgaag 1560
ctggattcta tgggcgtgta cagaatcctg gccatctata gcaccgtggc ctctagcctg 1620
gtgctggtgg tgagcctggg agcaatctcc ttttggatgt gctctaacgg cagcctgcag 1680
tgccgggtgt gcatc 1695
<210> 117
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 117
atggaggcca agctgttcgt gctgttttgc gccttcaccg ccctgaaggc cgacacaatc 60
tgcgtgggct accacgccaa caatagcacc gagacagtgg ataccgtgct ggagaagaac 120
gtggccgtga cacactccgt gaacctgctg gaggacaagc acaatggcaa gctgtgcaag 180
ctgggaggaa tcgcaccact gcacctgggc aagtgcaaca tcgccggctg gatcctgggc 240
aatcctgagt gtgagctgct gatcagcaag gagtcttgga gctacatcgt ggaggcccca 300
aactccgaca atggagcatg ctacccagga cagttcgcag attatgagga gctgcgggag 360
cacctgagct ccgtgtctag ctttgagaag ttcgagatct ttccaaaggc ctcctcttgg 420
cccaaccacg attctaataa gggagtgacc gcagcatgtc cttacgcagg cgccaacagc 480
ttttatagga atctgctgtg gatcgtgaag aagggcaaca gctacccaaa gatcaataag 540
tcctacatca acaataaggg caaggaggtg ctggtgctgt ggggaatcca ccacccacct 600
acctccacag accagcagtc tctgtacaag aacgccgatg cctacgtgtt cgtgggcacc 660
tcccggtact ctaagaagtt taagcctgag atcgccatca ggccaaaggt gcgcgaccag 720
gagggcagaa tgaattacta ttggaccctg ctggaccagg gcgataccat cacattcgag 780
gcaacaggaa acctggtggc accaagatat gcctttgcca tggagcgcaa tgccggcagc 840
ggcatcatca tctccgacac ccctgtgcac gattgcaaca ccacatgtca gacaccaaag 900
ggcgccatca acagcaatct gcccttccag aatgtgcacc ctatcaccat cggcgagtgt 960
ccaaagtacg tgcggtccac caagctgaga atggtgacag gcctgcggaa catccccagc 1020
gtgcagtcca gaggcctgtt cggagcaatc gcaggctttc tggagggagg atggacaggc 1080
atggtgaacg gctggtacgg ctatcaccac cagaatgacc agggctctgg ctatgccgcc 1140
gatcagaaga gcacccagat cgccatcgac ggcatctcta acaaggtcaa tagcgtgatc 1200
gagaagatga atatccagtt cacagccgtg ggcaaggagt ttagccacct ggagcggaga 1260
atcgagaacc tgaataagaa ggtggacgat ggcctgctgg acgtgtggac ctacaacgcc 1320
gagatgctgg tgctgctgga gaatgagagg acactggact tccacgactt caacgtgaag 1380
aatctgtacg agaaggtgaa gtcccagctg cgcaacaatg ccaaggagat cggcaacggc 1440
tgcttcgagt tttatcacaa gtgcgacaac acctgtatgg agtccgtgag gaatggcaca 1500
tacgattatc ctaagtacta tgaggagtct aagctgaatc gcgaggagat cgatggcgtg 1560
aagctggagt ccatgggcgt gtaccagatc ctggccatct attctaccgc agccagctcc 1620
ctggtgctgc tggtgtccct gggagccgtg tctttctgga tgtgctctaa cggcagcctg 1680
cagtgcaaga tctgtatc 1698
<210> 118
<211> 1713
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 118
atgaagacca tcatcgcctt ctcctgcatc ctgtgcctga tcttcgccca gaagctgccc 60
ggcagcgaca acaactccat ggccaccctg tgcctgggac accacgccgt gccaaacggc 120
accctggtga agaccatcac cgacgaccag atcgaggtga ccaacgccac cgagctggtg 180
cagtccagct ccaccggaag gatctgcaac agcccaggac accagatcct ggacggcaag 240
aactgcaccc tgatcgacgc tctgctgggc gacccacact gcgacgactt ccagaacaag 300
tgggacctgt tcgtggagcg cagcaccgcc tactccggca actgctaccc ctactacgtg 360
ccagactacg ccagcctgag ggagcagctg tccctggtgg ctagctccgg caccctggag 420
ttcacccagg agtccttcaa ctggaccggc gtggcccagg acggaagctc ctacgcttgc 480
aggaggggaa gcgtgaacaa cagcttcttc tccagactga actggctgta cctgaactac 540
aagtaccccg ccctgaacgt gaccatgcca aacaacgaca agttcgacaa gctgtacatc 600
tggggagtgc accacccagg caccgacaag gaccagacca acctgtacgt gcaggcctcc 660
ggaagggtgg tgagcaccaa gagatcccag cagaccgtga tccccaacat cggatccagg 720
ccatgggtgc ggggcgtgag ctccatcatc agcatctact ggaccatcgt gaagcccggc 780
gacatcctgc tgatcaactc caccggcaac ctgatcgccc caaggggcta cttcaagatc 840
cagagcggca agagctccgg catcatgaga agcgacgccc ccatcgactg caactccgag 900
tgcatcaccc caaacggcag catccccaac gacaagccat tccagaacgt gaacaggatc 960
acctacggcg cctgccccag atacgtgaag cagaacaccc tgaagctggc caccggcatg 1020
cgcaacgtgc cagagaagca gaccagggga atcttcggag ctatcgctgg cttcatcgag 1080
aacggctggg agggcatggt ggacggctgg tacggcttcc gccaccagaa ctccgagggc 1140
accggacagg ctgctgacct gaagagcacc caggccgcca tcaaccagat caccggcaag 1200
ctgaaccgcg tgatcaagaa gaccaacgag aagttccacc agatcgagaa ggagttctcc 1260
gaggtggagg gcagaatcca ggacctggag aagtacgtgg aggacaccaa gatcgacctg 1320
tggtcctaca acgccgagct gctggtggcc ctggagaacc agcacaccat cgacctgacc 1380
gacagcgaga tgaacaagct gttcgagagg accagaaagc agctgcgcga gaacgccgag 1440
gacatgggca acggctgctt caagatctac cacaagtgcg acaacgcctg catcggcagc 1500
atccggaacg gcacctacga ccacgacgtg taccgcgacg aggccctgaa caaccggttc 1560
cagatcaagg gcgtgcagct gaagtccggc tacaaggact ggatcctgtg gatcagcttc 1620
gccatctcct gcttcctgct gtgcgtggtg ctgctgggct tcatcagcat gtgggcctgc 1680
cagaagggca acatccggtg caacatctgc atc 1713
<210> 119
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 119
atgaagacca tcatcgcctt ctcctgcatc ctgtgcctga tcttcgccca gaagctgcca 60
ggaagcgaca actccaccgc caccctgtgc ctgggacacc acgccgtgcc aaacggcacc 120
atggtgaaga ccatcaccga cgaccaggtg gaggtgacca acgccaccga gctggtgcag 180
tccagctcca ccggaaggat ctgcaactcc ccacaccaga tcctggacgg caagaactgc 240
accctgatcg acagcctgct gggcgaccca cactgcgacg acttccagaa caaggagtgg 300
gacctgttcg tggagaggtc caaggcctac agcaactgct acccctacta cgtgccagac 360
tacgccaccc tgagatccct ggtggccagc tccggcaacc tggagttcac ccaggagagc 420
ttcaactgga ccggcgtggc ccaggacgga agctcctacg cttgcaggag gggcagcgtg 480
aactccttct tcagccggct gaactggctg tacaacctga actacaagta ccccgagcag 540
aacgtgacca tgccaaacaa cgacaagttc gacaagctgt acatctgggg agtgcaccac 600
ccaggcaccg acaaggacca gaccaacctg tacgtgcagg cctccggacg cgtgatcgtg 660
tccaccaaga gaagccagca gaccatcatc cccaacatcg gatccaggcc atgggtgcgg 720
ggcgtgagct ccatcatcag catccactgg accatcgtga agcccggcga catcctgctg 780
atcaacagca ccggaaacct gatcgctcca aggggctact tcaagatcca gaacggcaag 840
agctccatca tgcggtccga cgcccacatc gacgagtgca acagcgagtg catcaccccc 900
aacggcagca tctccaacga caagccattc cagaacgtga acaagatcac ctacggcgcc 960
tgccccaggt acatcaagca gaacaccctg aagctggcca ccggcatgag gaacatccca 1020
gagaagcaga ccagaggcat cttcggcgcc atcgccggct tcatcgagaa cggctgggag 1080
ggcatggtga acggctggta cggcttcaga caccagaact ccgagggcac cggacaggct 1140
gctgacctga agagcaccca ggccgccatc aaccagatca ccggcaagct gaaccgcgtg 1200
atcaagaaga ccaacgagaa gttccaccag atcgagaagg agttctccga ggtggaggga 1260
aggatccagg acctggagaa gtacgtggag gacaccaaga tcgacatctg gagctacaac 1320
gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgaccga ctccgagatg 1380
agcaagctgt tcgagcgcac caggagacag ctgcgggaga acgccgagga catcggcaac 1440
ggctgcttca agatctacca caagtgcgac aactcctgca tggagagcat caggaacggc 1500
acctacgacc acgacatcta ccgcaacgag gccctgaaca accggttcca gatcaagggc 1560
gtgcagctga agagcggcta caaggactgg atcctgtgga tctccttcgc catcagctgc 1620
ttcctgctgt gcgtggtgct gctgggcttc atcatgtggg cctgccagag gggcaacatc 1680
agatgcaaca tctgcgtg 1698
<210> 120
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 120
atgaagacca tcatcgcctt ctcctgcatc ctgtgcctga tcttcgccca gaagctgccc 60
ggcagcgaca actccatggc caccctgtgc ctgggacacc acgccgtgcc aaacggcacc 120
ctggtgaaga ccatcaccga cgaccagatc gaggtgacca acgccaccga gctggtgcag 180
tccagctcca ccggcaggat ctgcaacagc ccccaccaga tcctggacgg caagaactgc 240
accctgatcg acgctctgct gggcgaccca cactgcgacg acttccagaa caaggagtgg 300
gacctgttcg tggagcgcag caccgcctac agcaactgct acccctacta cgtgccagac 360
tacgccagcc tgcggtccct ggtggctagc tccggcaccc tggagttcac ccaggagtcc 420
ttcaactgga ccggcgtggc ccaggacgga agctcctacg cttgcaggag gggcagcgtg 480
aactccttct tcagcagact gaactggctg tacaacctga actacaagta ccccgagcag 540
aacgtgacca tgccaaacaa cgacaagttc gacaagctgt acatctgggg agtgcaccac 600
ccaggcaccg acaaggacca gaccaacctg tacgtgcagg cctccggacg cgtgatcgtg 660
tccaccaaga gaagccagca gaccgtgatc cccaacatcg gatccaggcc atgggtgcgg 720
ggcgtgagct ccatcatcag catctactgg accatcgtga agcccggcga catcctgctg 780
atcaacagca ccggcaacct gatcgcccca aggggctact tcaagatcca gtccggcaag 840
agctccatca tgagaagcga cgcccacatc gacgagtgca actccgagtg catcaccccc 900
aacggcagca tccccaacga caagccattc cagaacgtga acaggatcac ctacggcgcc 960
tgccccagat acgtgaagca gaacaccctg aagctggcca ccggcatgcg caacgtgcca 1020
gagaagcaga ccaggggaat cttcggagct atcgctggct tcatcgagaa cggctgggag 1080
ggcatggtgg acggctggta cggcttccgc caccagaact ccgagggcac cggacaggct 1140
gctgacctga agagcaccca ggccgccatc aaccagatca ccggcaagct gaaccgcgtg 1200
atcaagaaga ccaacgagaa gttccaccag atcgagaagg agttcagcga ggtggagggc 1260
agaatccagg acctggagaa gtacgtggag gacaccaaga tcgacctgtg gtcctacaac 1320
gccgagctgc tggtggccct ggagaaccag cacaccatcg acctgaccga cagcgagatg 1380
aacaagctgt tcgagaggac cagaaagcag ctgcgcgaga acgccgagga catgggcaac 1440
ggctgcttca agatctacca caagtgcgac aacgcctgca tcggctccat ccggaacggc 1500
acctacgacc acgacgtgta ccgcgacgag gccctgaaca accggttcca gatcaagggc 1560
gtgcagctga agagcggcta caaggactgg atcctgtgga tctccttcgc catcagctgc 1620
ttcctgctgt gcgtggtgct gctgggcttc atcatgtggg cctgccagaa gggcaacatc 1680
cggtgcaaca tctgcatc 1698
<210> 121
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 121
atgaagacca tcatcgccct gtcctacatc ctgtgcctgg tgttcgccca gaagctgcca 60
ggaaacgaca acaacatggc caccctgtgc ctgggacacc acgccgtgcc caacggcacc 120
atcgtgaaga ccatcaccaa cgaccagatc gaggtgacca acgccaccga gctggtgcag 180
tccaccagca agggcgagat ctgctccagc ccacaccaga tcctggacgg cgagaactgc 240
accctgatcg acgctctgct gggcgaccca cactgcgacg acttccagaa caagaagtgg 300
gacctgttcg tggagagatc caaggcccac agcaactgct acccctacta cgtgccagac 360
tacgccaccc tgcgctccct ggtggcttcc agcggcaacc tggagttcac ccaggagagc 420
ttcaactgga ccggagtgac ccaggacgga gcttccagcg cctgcaagcg ccggtccagc 480
aagtccttct tcagccgcct gaactggctg cacaacctga actacaagta cccagccctg 540
aacgtgacca tgcccaacaa cgacaacttc gacaagctgt acatctgggg cgtgcaccac 600
ccatccaccg acaaggacca gacctccctg tacatccagg ccagcggaag ggtgaccgtg 660
tccaccaaga ggagccagca ggccgtgatc ccaaacatcg gatccaggcc acgggtgagg 720
gacatcccct cccggatcag catctactgg accatcgtga ggccaggcga catcctgctg 780
atcaacagca acggcaacct gatcgccccc agaggctact tcaagatccg ctccggcaag 840
tccagcatca tgcggagcga cgcccccatc ggcaactgca acagcgagtg catcacccca 900
aacggctcca tcagcaacga caagcccttc cagaacgtga acaagatcac ctacggcgcc 960
tgcccaaggt acatcaagca gaacaccctg aagctggcca ccggcatgag aaacatcccc 1020
gagaagcaga ccaggggaat cttcggagct aaggctggct tcatcgagaa cggctgggag 1080
ggcatggtga acggctggta cggcttcaga caccagaact ccgagggaag gggacaggct 1140
gctgacctga agagcaccca ggccgccatc gaccagatca acggcaagct gaacagactg 1200
atcggcaaga ccaacgagaa gttccaccag atcgagaagg agttctccga gatcgagggc 1260
cggatccagg acctggagag gtacgtggag gacaccaaga tcgacatctg gagctacaac 1320
gccgagctgc tggtggccat ggagaaccag cacaccatcg acctgaccga ctccgagatg 1380
agcaagctgt tcgagagaac caggagacag ctgcgcgaga acgccgagga catgggcaac 1440
ggctgcctga agatctacca caagtgcgac aactcctgca tggagagcat ccggaacggc 1500
acctacgacc acgacatcta ccggaacgag gccctgaaca acaggttcca gatcaagggc 1560
gtggagctga agagcgagta caaggactgg atcctgtgga tctccttcgc caccagctgc 1620
ttcctgctgt gcgccgtgct gctgggcttc atcatgtggg cctgccagaa gggcaacatc 1680
aagtgcaaca tctgcatc 1698
<210> 122
<211> 1698
<212> DNA
<213> 人工
<220>
<223> 合成产生的编码序列
<400> 122
atgaaggcca tcatcgcctt ctcctgcatc ctgtgccaga tcttcgccca gaagctgccc 60
ggcaacgaca actccaccgc caccctgtgc ctgggacacc acgccgtgag caacggcacc 120
ctggtgaaga ccatcaccga cgaccaggtg gaggtgacca acgccaccga gctggtgcag 180
aacttctcca tgggcaagat ctgcaagaac ccccacagga tcctggacgg cgccaactgc 240
accctgatcg acagcctgct gggcgaccca cactgcgacg gcttccagaa cgagaagtgg 300
gacctgttca tcgagaggtc caaggccttc agcaactgct acccctacga cgtgccagac 360
tacgcctccc tgagaagcct gatcgcctcc agcggcaccc tggagttcaa caacgagtcc 420
ttcaactgga ccggagtgac ccagaacgga ggatccagcg cctgcaagag gggacccaac 480
aactccttct tcagcagact gaactggctg acccacctga acttcaagta ccccgccctg 540
gaggtgacca tgccaaacaa cgagcagttc gacaagctgt acatctgggg agtgcaccac 600
ccagctaccg acaaggacca gatcagcctg tacgctcagg ctgctggaag gatcatcgtg 660
tccaccaaga gaagccagca gaccatcatc cccaacatcg gctccaggcc atggatcaga 720
ggcgtgtcca gcatcatcag catccactgg accatcgtga agcccggcga catgctgctg 780
atcaactcca ccggcaacat catcgcccca cgcggctact tcaagatcca gaccggcaag 840
tccagcgtga tgcggagcga cgctccaatc ggcaagtgca actccgcctg catcacccca 900
aacggaagca tccccaacga ccgcccattc cagaacgtga acaagatcac ctacggcgcc 960
tgccccaagt acgtgaagca gaacaccctg aagctggcca ccggcatgag gaacatccca 1020
gagaggcaga ccaggggaat cttcggagct atcgccggct tcatcgagaa cggctgggag 1080
ggcatgatcg acggctggta cggcttccgc caccacaact ccgagggcac cggacaggct 1140
gctgacatca agagcaccca ggctgccatc aaccagatca acggcaagct gaaccgcgtg 1200
atcgagaaga ccaacgagaa gttccaccag atcgagaagg agttcctgga ggtggagggc 1260
cggatccagg acatcgagaa gtacatcgag gacaccaaga tcgacctgtg gtcctacaac 1320
gccgagctgc tgatcgccct ggagaaccag cacaccatcg acctggccga cagcgagatg 1380
aacaagctgt tcgagaagac caagaagcag ctgagggaga acgccgagga catcggcaac 1440
ggctgcttca agatctacca caagtgcgac aacacctgca tcggcagcat cagaaacggc 1500
acctacaacc acgacgtgta ccgcgacgag gccgtgaaca accggttcca gatcaagggc 1560
gtgcagctga agctgggcta caaggactgg atcctgtgga tctccttcgc catgagctgc 1620
ttcctgctgt gcgtggccct gctgggcttc atcatgtggg cttgccagag gggaaacatc 1680
aggtgcaaca tctgcgtg 1698

Claims (23)

1.一种疫苗多肽，其与选自SEQ ID NO:1-102中任一个的氨基酸序列具有至少90％序列同一性。
2.根据权利要求1所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:1-102中任一个的氨基酸序列具有至少95％序列同一性。
3.根据权利要求1或2所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:1-102中任一个的氨基酸序列具有至少99％序列同一性。
4.根据权利要求1、2或3所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:1-102中任一个的氨基酸序列具有100％序列同一性。
5.根据权利要求1所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95和100-102的氨基酸序列具有至少90％序列同一性。
6.根据权利要求1、2或5所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95和100-102中任一个的氨基酸序列具有至少95％序列同一性。
7.根据权利要求1、2、3、5或6所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95和100-102中任一个的氨基酸序列具有至少99％序列同一性。
8.根据权利要求1-7中任一项所述的疫苗多肽，其中所述多肽与选自SEQ ID NO:6-8、13-15、20-22、27-29、34-36、41-43、49-52、57-60、65-67、72-74、79-81、86-88、93-95和100-102中任一个的氨基酸序列具有100％序列同一性。
9.根据权利要求1-8中任一项所述的疫苗多肽，其中所述多肽是由与选自SEQ ID NO:103-122的核酸序列具有至少90％序列同一性的核酸序列编码的。
10.根据权利要求1-9中任一项所述的疫苗多肽，其中所述多肽是由与选自SEQ ID NO:103-122的核酸序列具有至少95％序列同一性的核酸序列编码的。
11.根据权利要求1-10中任一项所述的疫苗多肽，其中所述多肽是由与选自SEQ IDNO:103-122的核酸序列具有至少99％序列同一性的核酸序列编码的。
12.根据权利要求1-11中任一项所述的疫苗多肽，其中所述多肽是由与选自SEQ IDNO:103-122的核酸序列具有至少100％序列同一性的核酸序列编码的。
13.一种疫苗组合物，其包含至少一种根据权利要求1-12中任一项所述的疫苗多肽和递送媒介物。
14.根据权利要求13所述的疫苗组合物，其包含至少两种所述疫苗多肽。
15.根据权利要求13所述的疫苗组合物，其包含至少三种所述疫苗多肽。
16.根据权利要求13所述的疫苗组合物，其中所述疫苗多肽选自SEQ ID NO:3、6、17、20、46、49、54和57。
17.根据权利要求13-16中任一项所述的疫苗组合物，其中所述递送媒介物是病毒。
18.根据权利要求17所述的疫苗组合物，其中所述递送媒介物病毒选自腺病毒、腺相关病毒、逆转录病毒、甲病毒、副粘病毒和弹状病毒。
19.根据权利要求13所述的疫苗组合物，其中所述递送媒介物是纳米颗粒。
20.根据权利要求19所述的疫苗组合物，其中所述纳米颗粒是脂质纳米颗粒。
21.一种向受试者接种疫苗的方法，所述方法包括：
将根据权利要求1-12中任一项所述的疫苗多肽或根据权利要求13-20中任一项所述的疫苗组合物施用于需要疫苗接种的受试者。
22.根据权利要求21所述的方法，其中所述受试者选自人和猪。
23.根据权利要求21或22所述的方法，其中所述施用步骤重复多于一次。
CN201980074988.7A 2018-09-21 2019-09-20 制备和使用通用中心化流感疫苗基因的方法 Pending CN113423421A (zh)

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