CN113403200A

CN113403200A - Cell culture transfer heat preservation device

Info

Publication number: CN113403200A
Application number: CN202110725223.4A
Authority: CN
Inventors: 张国昀; 何彩云; 张建国; 段爱国
Original assignee: Research Institute of Forestry of Chinese Academy of Forestry
Current assignee: Research Institute of Forestry of Chinese Academy of Forestry
Priority date: 2021-06-29
Filing date: 2021-06-29
Publication date: 2021-09-17
Anticipated expiration: 2041-06-29
Also published as: CN113403200B

Abstract

The invention discloses a cell culture transfer heat preservation device which comprises a box body, a box cover, a lock catch and a heat preservation layer arranged in the box body and the box cover, wherein the bottom of the side surface of the box cover is hinged with the upper part of the side surface of the box body through a hinge, the lock catch is positioned on the front surface of the box body, a self-balancing assembly is movably arranged in the box body, an air circulation assembly is arranged at the top of the box cover, and a temperature control assembly is arranged at the bottom of the box body. According to the invention, the self-balancing assembly is arranged, so that the cell culture dish placed in the placing box is always in a horizontal state, the stability of cell transfer is ensured, the weight of the first battery pack, the motor and the rotor is greater than that of the cell culture dish and cells to be cultured, and the heavier end of the first battery pack is positioned below the axis of the movable shaft, so that the stability of the cell culture dish when the box body is inclined is further improved; when the box body is shaken or overturned, the motor drives the rotor to rotate, so that the culture dish is ensured to be in relative level.

Description

Cell culture transfer heat preservation device

Technical Field

The invention relates to the technical field of transfer heat preservation in cell culture. In particular to a transfer heat preservation device for cell culture.

Background

Cell culture means simulating the growth environment of cells to enable the cells to survive, grow and reproduce and maintain the main structure and function; in the process of cell culture, the cells are sometimes required to be transported, in the process of transport, especially for long-time transport, the safety of the cells is difficult to guarantee, and once the transport box falls, rolls and the like, cultured cells placed in the transport box are easy to be damaged and the like, so that adverse effects are brought to the cell culture; different environments are needed for culturing different cells, if some plant cells need proper illumination and proper gas environment for culturing, and if some plant cells do not need the proper illumination and gas environment, the existing transfer box can not be adjusted; the temperature of traditional heat preservation device is generally carried out the constant temperature through heat sink, in case the heat sink can't work, just can not guarantee the temperature in the transfer case, and the heat resistance of cell is far less than cold tolerance, in case the temperature is too high, directly leads to the cell death.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a cell culture transfer heat preservation device which has a good constant temperature effect and good stability and can be adjusted by environment.

In order to solve the technical problems, the invention provides the following technical scheme: cell culture transfer heat preservation device, including box, case lid, hasp and the heat preservation of setting in box and case lid, the side bottom of case lid is articulated through the side upper portion of hinge with the box, the hasp is located the front of box, the box activity is provided with the self-balancing subassembly, the top of case lid is provided with the air cycle subassembly, the bottom of box is provided with the temperature control subassembly, the inner wall side of box is provided with first temperature sensor, carbon dioxide sensor and oxygen sensor respectively, first temperature sensor, carbon dioxide sensor and oxygen sensor all pass through wire and air cycle subassembly electric connection.

The cell culture transfer heat preservation device comprises an air pipe, a circulating fan, a main control board and a protective cover, wherein the air pipe, the circulating fan and the main control board are all arranged in the protective cover, the second end of the air pipe is communicated with an air inlet of the circulating fan, an ultraviolet lamp is arranged on the inner side wall of the air pipe, the first end of the air pipe is communicated with an upper air draft plate, an air outlet of the circulating fan is communicated with a lower air draft plate through a pipeline, an oxygen cylinder and a carbon dioxide cylinder are fixedly connected to the side surface of the protective cover through a fixed seat respectively, electromagnetic valves are communicated with bottle openings of the oxygen cylinder and the carbon dioxide cylinder respectively, the oxygen cylinder and the carbon dioxide cylinder are communicated with the air inlet of the circulating fan through the electromagnetic valves respectively, and the circulating fan, the ultraviolet lamp, the first temperature sensor, the carbon dioxide sensor and the oxygen sensor are electrically connected with the main control board through wires, the electromagnetic valve communicated with the oxygen cylinder opening and the electromagnetic valve communicated with the carbon dioxide cylinder opening are electrically connected with the main control board through leads; the bottom of safety cover and the top fixed connection of case lid, equal fixed connection in the top of case lid of tuber pipe, circulating fan and main control board.

Above-mentioned transfer heat preservation device is cultivated to cell, go up the convulsions board and arrange the structure of aerofoil down the same, go up the convulsions board and include the casing, a plurality of wind holes have evenly been seted up to the bottom of casing, the top intercommunication of casing has the trachea, the trachea of going up the convulsions board communicates with the first end of tuber pipe, the trachea and the circulating fan gas outlet intercommunication of lower aerofoil, the both sides of lower aerofoil and the inner wall both sides fixed connection of box, the top of going up the convulsions board and the inner wall top fixed connection of box, just the wind hole of going up the convulsions board sets up with the wind hole of lower aerofoil relatively.

The cell culture transfer heat preservation device comprises a self-balancing assembly, wherein the self-balancing assembly comprises an outer support ring, a middle support ring and an inner support ring, the diameters of the outer support ring, the middle support ring and the inner support ring are sequentially reduced and coaxially arranged on the same plane, movable shafts are arranged on the left side and the right side of the outer wall of the outer support ring and on the front side and the rear side of the inner wall of the outer support ring, the movable shafts on the left side and the right side of the outer wall of the outer support ring are vertical to the movable shaft axes on the front side and the rear side of the inner wall of the outer support ring, the inner wall of the outer support ring is movably connected with the outer side wall of the middle support ring through the two movable shafts, the movable shafts are also arranged on the left side and the right side of the inner wall of the middle support ring, the movable shafts on the left side and the right side of the inner wall of the middle support ring are coaxial with the movable shafts, the inner support ring is movably connected with a longitudinal buffer assembly on the front side and the rear side of the inner wall of the inner support ring, the end parts of the two longitudinal buffer assemblies are vertically and fixedly connected with a transverse buffer assembly, and the opposite surfaces of the two transverse buffer assemblies are fixedly connected with a fixed cylinder;

the middle part in the fixed cylinder is provided with a motor, an output shaft of the motor is connected with a rotor in a transmission manner, the upper end and the lower end of the motor are fixedly connected with the side surface of the inner wall of the fixed cylinder through a fixed frame, the middle part of the fixed frame is fixedly connected with a first battery pack, the bottom of the inner wall of the fixed cylinder is provided with a control panel, the bottom of the fixed cylinder is provided with an acceleration sensor, the first battery pack is electrically connected with the control panel through a wire, the control panel is respectively and electrically connected with the acceleration sensor and the motor through wires, the top of the fixed cylinder is fixedly connected with a bracket, the top of the bracket is movably connected with a placing box, an air bag is arranged between the bottom of the placing box and the top of the bracket, the; and the movable shafts on two sides of the outer wall of the outer support ring are respectively movably connected with two sides of the inner wall of the box body.

In the cell culture transfer heat preservation device, the longitudinal buffer assembly comprises a second supporting block, a second piston rod and a sleeve, one end of the second piston rod is slidably connected in the sleeve, the other end of the second piston rod is fixedly connected with one end of the second supporting block, the end part of the piston rod and the top of the inner wall of the sleeve are fixedly connected with a second magnetic block, the two second magnetic blocks are mutually exclusive, bearing holes are formed in the top of the sleeve and the bottom of the second supporting block, a bearing is arranged in each bearing hole, a rotating shaft is movably arranged in each bearing in a penetrating manner, an air hole is formed in the side wall of the sleeve, and an exhaust assembly is arranged on the side surface of each air hole; the rotating shaft at the bottom of the second supporting block is fixedly connected with the inner wall of the inner supporting ring;

the transverse buffer assembly comprises a connecting block, first piston rods fixedly connected with two ends of the connecting block respectively and first supporting blocks slidably connected with the two first piston rods respectively, the end parts of the first piston rods are inserted into piston holes in the side faces of the first supporting blocks, first magnetic blocks are fixedly connected to the end parts of the two first piston rods and the bottoms of the piston holes of the two fixing blocks respectively, the first magnetic blocks at the end parts of the piston rods and the first magnetic blocks at the bottoms of the piston holes of the fixing blocks are mutually exclusive, air holes are formed in the side walls of the first supporting blocks, and an exhaust assembly is arranged at the air holes; the rotating shaft at the bottom of the sleeve of the longitudinal buffering assembly is fixedly connected with the side wall of the connecting block, and the side wall of the first supporting block is fixedly connected with the outer side wall of the fixed cylinder.

Above-mentioned transfer heat preservation device is cultivated to cell, exhaust subassembly includes the soft board, the through-hole with the gas pocket intercommunication is seted up at the middle part of soft board, is provided with interior filter screen in the through-hole, the outside of soft board is provided with the lateral wall and the telescopic outer wall fixed connection of outer filter screen and outer filter screen, the upper portion and the telescopic lateral wall fixed connection of soft board.

The cell culture transfer heat preservation device comprises a liquid storage tank and a heat exchange pipe communicated with the upper part of the liquid storage tank, a liquid pump communicated with the lower part of the liquid storage tank, a semiconductor refrigeration component tightly attached to the bottom of the liquid tank and a heat exchanger, the second end of the heat exchange tube is in fluid communication with the liquid outlet of the heat exchanger, the liquid outlet of the liquid pump is in fluid communication with the liquid inlet of the heat exchanger, the bottom of the liquid storage tank is fixedly connected with a fixed plate, the side surface of the fixed plate is provided with a storage tank, liquefied gas is stored in the storage tank, a gas outlet of the storage tank is communicated with a gas inlet of the heat exchanger through two pipelines, an electromagnetic exhaust valve is arranged in the middle of one pipeline communicated with the storage tank, a manual valve is arranged in the middle of the other pipeline, and an electric heating rod and a second temperature sensor are arranged in the liquid storage tank; the both sides of liquid reserve tank respectively with the inner wall both sides fixed connection of box, the surface of liquid reserve tank is provided with the heat preservation, the heat exchange tube is the heliciform and coils the inner wall side at the box.

According to the cell culturing, transferring and heat insulating device, the bottom of the inner wall of the box body is provided with the second battery pack, the liquid pump, the electromagnetic exhaust valve, the second temperature sensor, the electric heating rod and the semiconductor refrigerating assembly are respectively and electrically connected with the main control board of the air circulation assembly through wires, the lower part of the side wall of the box body is provided with the air holes, the side surface of the inner wall of the box cover is provided with the light supplementing lamp and the exhaust valve, the top of the box cover is provided with the display screen and the control button, the display screen, the control button and the light supplementing lamp are all electrically connected with the main control board of the air circulation assembly through wires, the top of the box cover is provided with the transparent observation window, and the top of the transparent observation window is provided with the cover; supporting legs are arranged at four corners of the bottom of the box body.

The technical scheme of the invention achieves the following beneficial technical effects:

1. according to the invention, through arranging the self-balancing assembly, the cell culture dish placed in the placing box can be ensured to be always in a horizontal state, through the mutual matching and matching of the outer support ring, the middle support ring and the inner support ring, the box body can be ensured to incline, incline or turn over in any direction, and the culture dish can be ensured to be always in the horizontal state, so that the stability of cell transportation is ensured, the weight of the first battery pack, the motor and the rotor is more than that of the culture dish and cultured cells, and the heavier end is positioned below the axis of the movable shaft, so that the gravity center is reduced, and the stability of the culture dish when the box body inclines is further improved; through cooperation motor and rotor, when the box takes place stronger rocking or upset, the motor drives the rotor and rotates, and as is known, high-speed pivoted object has certain inertia, and inertia can stabilize the bracket, avoids it along with the box upset, guarantees that the culture dish is in relative level.

2. The invention can provide longitudinal and transverse buffer effects by arranging the longitudinal buffer component and the transverse buffer component, the transverse buffer component and the longitudinal buffer component can realize multidirectional buffer effects by mutual matching, the transverse buffer component and the longitudinal buffer component both utilize the mutual repulsive force of the magnetic blocks to provide buffer force, the components which rub with each other are fewer, the resistance is smaller, the vibration is more sensitive to be absorbed, better buffer effect is provided, the structure is relatively simple, the service life is longer, meanwhile, the air bag can provide buffer in the vertical direction, so that the buffer effect is further improved, the exhaust components are arranged on the side surfaces of the buffer component and the longitudinal buffer component, when the piston rod moves inwards, the air in the piston cavity is compressed, the soft plate is pushed outwards to be opened, the air in the sleeve is rapidly exhausted, when the piston rod moves reversely under the repulsive force of the magnetic blocks, the soft board resets under self elasticity, and outside air enters the sleeve through the hole in the side of the soft board, so that the air entering speed is limited, and a certain damping effect is provided.

3. According to the invention, the air circulation assembly is arranged, so that air in the box body can circularly flow, the temperature of the box body is more uniform, the temperature unevenness is avoided, the oxygen sensor and the carbon dioxide sensor are matched, the oxygen and carbon dioxide concentrations in the box body can be monitored, the oxygen cylinder and the carbon dioxide cylinder are matched, the oxygen and carbon dioxide concentrations in the box body can be regulated and controlled, the gas environment in the box body is more suitable for cell culture, the brightness of the light supplement lamp can be regulated according to the requirement, and the brightness in the box body is regulated; when the air through circulating fan to the box circulates, the air passes through the trachea, through the irradiation of the interior ultraviolet lamp of trachea, disinfects to the air to be in aseptic environment relatively in the assurance box, and the ultraviolet ray can not shine the cell of cultivating, guarantees that the cell can normally be cultivated.

4. According to the invention, through arranging the semiconductor refrigeration assembly, the liquid in the liquid storage tank can be cooled, and the cold liquid is circulated into the heat exchange pipe under the action of the liquid pump, so that the temperature in the tank body is reduced, meanwhile, the temperature in the tank body can be monitored by the first temperature sensor, so that the temperature in the tank body is suitable for cell culture, when the semiconductor refrigeration assembly cannot work, liquefied gas in the storage tank can be discharged by opening the electromagnetic exhaust valve, a large amount of heat can be absorbed when the liquefied gas is gasified, and when the gas passes through the heat exchanger, the heat of the liquid is absorbed, so that the temperature in the tank body is reduced, the temperature in the tank body is ensured not to be overhigh, and the two refrigeration modes are matched, so that the use stability of the heat preservation tank is improved; when the temperature is too low, resulting in the liquid temperature in the liquid reserve tank, accessible electric heating rod is to liquid heating to the temperature that the temperature is in suitable cell culture in the assurance box.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic side view of the cross-sectional structure of the present invention;

FIG. 3 is a schematic cross-sectional view of an air duct according to the present invention;

FIG. 4 is a schematic top view of a self-balancing assembly of the present invention;

FIG. 5 is a schematic cross-sectional front view of a self-balancing assembly of the present invention;

FIG. 6 is a schematic front sectional view of a longitudinal cushioning element of the present invention;

FIG. 7 is an enlarged view of FIG. 6A of the present invention;

FIG. 8 is a schematic top cross-sectional view of a lateral cushioning assembly of the present invention;

FIG. 9 is a schematic cross-sectional view of an upper air draft plate according to the present invention.

The reference numbers in the figures denote: 1-a box body; 2-box cover; 3-self-balancing assembly; 301-an outer support ring; 302-middle support ring; 303-inner support ring; 304-a movable shaft; 305-a longitudinal cushioning component; 306-a lateral cushioning assembly; 307-a stationary cartridge; 308-a fixing frame; 309-a motor; 310-a first battery pack; 311-control panel; 312-an acceleration sensor; 313-a rotor; 314-a bracket; 315-balloon; 316-placing the box; 317-box cover; 318-connecting block; 319-first piston rod; 320-a first support block; 321-a first magnetic block; 322-an exhaust assembly; 323-a second piston rod; 324-a second support block; 325-a sleeve; 326 — second magnetic block; 327-air holes; 328-soft board; 329-inner filtering net; 330-outer filter screen; 4-an air circulation assembly; 401-a protective cover; 402-an air duct; 403-ultraviolet lamp; 404-a circulating fan; 405-a main control board; 406-an oxygen cylinder; 407-carbon dioxide cylinder; 5, locking and buckling; 6-observation window; 7-an exhaust valve; 8-a first temperature sensor; 9-a carbon dioxide sensor; 10-an oxygen sensor; 11-upper suction plate; 111-a housing; 112-wind holes; 113-trachea; 12-lower exhaust plate; 13-heat exchange tube; 14-insulating layer; 15-supporting feet; 16-a liquid storage tank; 17-a second temperature sensor; 18-a fill light; 19-a semiconductor refrigeration assembly; 20-air holes; 21-fixing the plate; 22-a storage tank; 23-a heat exchanger; 24-a liquid pump; 25-electromagnetic exhaust valve; 26-a manual valve; 27-a temperature control assembly; 28-an electrical heating rod; 29-a second battery pack.

Detailed Description

Referring to fig. 1-2, the cell culturing, transferring and heat preserving device comprises a box body 1, a box cover 2, a lock catch 5 and a heat preserving layer 14 arranged in the box body 1 and the box cover 2, wherein the bottom of the side surface of the box cover 2 is hinged with the upper part of the side surface of the box body 1 through a hinge, the lock catch 5 is positioned on the front surface of the box body 1, the lower part of the side wall of the box body 2 is provided with an air vent 20, the side surface of the inner wall of the box cover 2 is provided with a light supplementing lamp 18 and an exhaust valve 7, the top of the box cover 2 is provided with a display screen and a control button, the display screen, the control button and the light supplementing lamp 18 are electrically connected with a main control board 405 of an air circulation assembly 4 through wires, the top of the box cover 2 is provided with a transparent observation window 6, the top of the transparent observation window 6 is provided with a cover, the bottom of the box body 1 is provided with four corners 15, and a self-balancing assembly 3 is movably arranged in the box body 1, the top of the box cover 2 is provided with an air circulation assembly 4, the air circulation assembly 4 comprises an air pipe 402, a circulating fan 404, a main control board 405 and a protective cover 401, the air pipe 402, the circulating fan 404 and the main control board 405 are all arranged in the protective cover 401, the second end of the air pipe 402 is communicated with an air inlet of the circulating fan 404, the inner side wall of the air pipe 402 is provided with an ultraviolet lamp 403, when the air of the box body 1 is circulated through the circulating fan 404, the air passes through the air pipe 113 and is sterilized through irradiation of the ultraviolet lamp 403 in the air pipe 113, so that the inside of the box body 1 is in a relatively sterile environment, ultraviolet rays cannot irradiate cultured cells, and the cells can be normally cultured, the first end of the air pipe 402 is communicated with the upper exhaust plate 11, the air outlet of the circulating fan 404 is communicated with the lower exhaust plate 12 through a pipeline, as shown in fig. 9, the structure of the upper air draft plate 11 is the same as that of the lower air draft plate 12, the upper air draft plate 11 comprises a shell 111, a plurality of air holes 112 are uniformly formed in the bottom of the shell 111, an air pipe 113 is communicated with the top of the shell 111, the air pipe 113 of the upper air draft plate 11 is communicated with the first end of an air pipe 402, the air pipe 113 of the lower air draft plate 12 is communicated with the air outlet of a circulating fan 404, two sides of the lower air draft plate 12 are fixedly connected with two sides of the inner wall of the box body 1, the top of the upper air draft plate 11 is fixedly connected with the top of the inner wall of the box body 1, the air holes 112 of the upper air draft plate 11 are arranged opposite to the air holes 112 of the lower air draft plate 12, the side of the protective cover 401 is fixedly connected with an oxygen cylinder 406 and a carbon dioxide cylinder 407 through fixing seats respectively, the bottle openings of the oxygen cylinder 406 and the carbon dioxide cylinder 407 are communicated with electromagnetic valves respectively, and the oxygen cylinder 406 and the carbon dioxide cylinder 407 are communicated with the air inlet of the circulating fan 404 through the electromagnetic valves 407 respectively, the circulating fan 404, the ultraviolet lamp 403, the first temperature sensor 8, the carbon dioxide sensor 9 and the oxygen sensor 10 are all electrically connected with the main control board 405 through leads, and the electromagnetic valve communicated with the mouth of the oxygen cylinder 406 and the electromagnetic valve communicated with the mouth of the carbon dioxide cylinder 407 are all electrically connected with the main control board 405 through leads; the bottom of safety cover 401 and the top fixed connection of case lid 2, tuber pipe 402, the equal fixed connection of circulating fan 404 and main control board 405 at the top of case lid 2, through setting up air circulation subassembly 4, can make the air in the box 1 carry out the circulation flow, thereby make the temperature of box 1 more even, avoid appearing the temperature inhomogeneous, cooperation oxygen sensor 10 and carbon dioxide sensor 9, can monitor oxygen and carbon dioxide concentration in the box 1, and cooperation and oxygen cylinder 406 and carbon dioxide gas cylinder 407, can regulate and control oxygen and carbon dioxide concentration in the box 1, make the gaseous environment more suitable cell culture in the box 1, and can adjust the luminance of lamp 18 as required, thereby adjust the luminance in the box 1.

The bottom of the box body 1 is provided with a temperature control assembly 27, the temperature control assembly 27 comprises a liquid storage box 16, a heat exchange pipe 13 communicated with the upper part of the liquid storage box 16, a liquid pump 24 communicated with the lower part of the liquid storage box 16, a semiconductor refrigeration assembly 19 tightly attached to the bottom of the liquid storage box and a heat exchanger 23, the liquid in the liquid storage box 16 can be cooled by arranging the semiconductor refrigeration assembly 19, and the cold liquid is circulated into the heat exchange pipe 13 under the action of the liquid pump 24, so that the temperature in the box body 1 is reduced, meanwhile, the temperature in the box body 1 can be monitored by a first temperature sensor 8, so that the temperature in the box body 1 is suitable for cell culture, the second end of the heat exchange pipe 13 is communicated with the liquid outlet fluid of the heat exchanger 23, the liquid outlet of the liquid pump 24 is communicated with the liquid inlet fluid of the heat exchanger 23, the bottom of the liquid storage box 16 is fixedly connected with a fixing plate 21, the side of the fixed plate 21 is provided with a storage tank 22, liquefied gas is stored in the storage tank 22, a gas outlet of the storage tank 22 is communicated with a gas inlet of a heat exchanger 23 through two pipelines, the middle part of one pipeline communicated with the storage tank 22 is provided with an electromagnetic exhaust valve 25, the middle part of the other pipeline is provided with a manual valve 26, when the semiconductor refrigeration component 19 cannot work, the electromagnetic exhaust valve 25 can be opened, the liquefied gas in the storage tank 22 is discharged, the liquefied gas can absorb a large amount of heat during gasification, and when the gas passes through the heat exchanger 23, the heat of the liquid is absorbed, so that the liquid is cooled, the temperature in the box body 1 is ensured not to be too high, the two refrigeration modes are matched, the use stability of the insulation box is improved, the electric heating rod 28 and the second temperature sensor 17 are arranged in the liquid storage box 16, when the temperature is too low, and the temperature of the liquid in the liquid storage box 16 is caused, the liquid can be heated by the electric heating rod 28, so that the temperature in the box body 1 is ensured to be at a temperature suitable for cell culture; the both sides of liquid reserve tank 16 respectively with the inner wall both sides fixed connection of box 1, the surface of liquid reserve tank 16 is provided with heat preservation 14, heat exchange tube 13 is the heliciform and coils in the inner wall side of box 1, the inner wall side of box 1 is provided with first temperature sensor 8, carbon dioxide sensor 9 and oxygen sensor 10 respectively, first temperature sensor 8, carbon dioxide sensor 9 and oxygen sensor 10 all pass through wire and 4 electric connection of air cycle subassembly, the inner wall bottom of box 1 is provided with second group battery 29, liquid pump 24, electromagnetic exhaust valve 25, second temperature sensor 17, electric heating rod 28 and semiconductor refrigeration subassembly 19 are respectively through wire and air cycle subassembly 4's main control board 405 electric connection.

As shown in fig. 4-5, the self-balancing assembly 3 includes an outer support ring 301, a middle support ring 302 and an inner support ring 303, diameters of the outer support ring 301, the middle support ring 302 and the inner support ring 303 are sequentially reduced and coaxially disposed on the same plane, movable shafts 304 are disposed on left and right sides of an outer wall of the outer support ring 301 and on front and rear sides of an inner wall of the outer support ring 301, the movable shafts 304 on left and right sides of the outer wall of the outer support ring 301 are perpendicular to axes of the movable shafts 304 on front and rear sides of the inner wall of the outer support ring 301, an inner wall of the outer support ring 301 is movably connected with an outer wall of the middle support ring 302 through the two movable shafts 304, the movable shafts 304 are disposed on left and right sides of the inner wall of the middle support ring 302, the movable shafts 304 on left and right sides of the inner wall of the middle support ring 302 are coaxial with the movable shafts 304 on left and right sides of the outer support ring 301, the inner wall of the middle support ring 302 is movably connected with the inner support ring 303 through the movable shafts 304, the front side and the rear side of the inner wall of the inner support ring 303 are movably connected with longitudinal buffer components 305, the end parts of the two longitudinal buffer components 305 are vertically and fixedly connected with transverse buffer components 306, the opposite surfaces of the two transverse buffer components 306 are fixedly connected with a fixed cylinder 307, the middle part in the fixed cylinder 307 is provided with a motor 309, the output shaft of the motor 309 is in transmission connection with a rotor 313, the upper end and the lower end of the motor 309 are fixedly connected with the side surface of the inner wall of the fixed cylinder 307 through a fixed frame 308, the middle part of the fixed frame 308 is fixedly connected with a first battery pack 310, the bottom of the inner wall of the fixed cylinder 307 is provided with a control panel 311, the bottom of the fixed cylinder 307 is provided with an acceleration sensor 312, the first battery pack 310 is electrically connected with the control panel 311 through a lead, and the control panel 311 is electrically connected with the acceleration sensor 312 and the motor 309 through leads respectively, the top of the fixed cylinder 307 is fixedly connected with a bracket 314, the top of the bracket 314 is movably connected with a placing box 316, an air bag 315 is arranged between the bottom of the placing box 316 and the top of the bracket 314, the top of the placing box 316 is buckled with a box cover 317, and the top of the box cover 317 is provided with a through hole; the movable shafts 304 on the two sides of the outer wall of the outer support ring 301 are movably connected with the two sides of the inner wall of the box body 1 respectively, the cell culture dish placed in the placing box 316 can be ensured to be in a horizontal state all the time by arranging the self-balancing assembly 3, the box body 1 can be ensured to incline, incline or turn over towards any direction by the mutual matching of the outer support ring 301, the middle support ring 302 and the inner support ring 303, and the culture dish can be ensured to be in a horizontal state all the time, so that the stability of cell transfer is ensured, the weight of the first battery pack 310, the motor 309 and the rotor 313 is greater than that of the culture dish and cultured cells, and the heavier end is positioned below the axis of the movable shaft 304, so that the center of gravity is reduced, and the stability of the culture dish when the box body 1 inclines is; through cooperation motor 309 and rotor 313, when box 1 took place stronger rocking or upset, motor 309 drove rotor 313 and rotates, and as is known, high-speed pivoted object has certain inertia, and inertia can stabilize bracket 314, avoids it to overturn along with box 1, guarantees that the culture dish is in relative level.

As shown in fig. 6, the longitudinal buffer assembly 305 includes a second supporting block 324, a second piston rod 323, and a sleeve 325, one end of the second piston rod 323 is slidably connected in the sleeve 325, the other end of the second piston rod 323 is fixedly connected with one end of the second supporting block 324, the end of the piston rod and the top of the inner wall of the sleeve 325 are both fixedly connected with a second magnetic block 326, the two second magnetic blocks 326 are mutually exclusive, the top of the sleeve 325 and the bottom of the second supporting block 324 are both provided with bearing holes, a bearing is disposed in the bearing hole, a rotating shaft is movably inserted in the bearing, an air hole 327 is formed in a side wall of the sleeve 325, and an exhaust assembly 322 is disposed on a side surface of the air hole; the rotating shaft at the bottom of the second supporting block 324 is fixedly connected with the inner wall of the inner supporting ring 303, as shown in fig. 8, the transverse buffer component 306 comprises a connecting block 318, first piston rods 319 respectively fixedly connected with two ends of the connecting block 318, and first supporting blocks 320 respectively slidably connected with the two first piston rods 319, ends of the first piston rods 319 are inserted into piston holes in the side surfaces of the first supporting blocks 320, the ends of the two first piston rods 319 and the bottoms of the piston holes of the two fixing blocks are fixedly connected with first magnetic blocks 321, the first magnetic blocks 321 at the ends of the piston rods and the first magnetic blocks 321 at the bottoms of the piston holes of the fixing blocks are mutually repelled, air holes 327 are formed in the side walls of the first supporting blocks 320, and an exhaust component 322 is arranged at the air holes 327; the rotating shaft at the bottom of the sleeve 325 of the longitudinal buffer assembly 305 is fixedly connected with the side wall of the connecting block 318, the side wall of the first supporting block 320 is fixedly connected with the outer side wall of the fixed cylinder 307, as shown in fig. 7, the exhaust assembly 322 comprises a soft plate 328, the middle of the soft plate 328 is provided with a through hole communicated with the air hole 327, an inner filter screen 329 is arranged in the through hole, the outer side of the soft plate 328 is provided with an outer filter screen 330, the side wall of the outer filter screen is fixedly connected with the outer wall of the sleeve 325, the upper part of the soft plate 328 is fixedly connected with the side wall of the sleeve 325, longitudinal and

transverse buffer assemblies

305 and 306 are arranged to provide longitudinal and transverse buffer effects, the transverse buffer assemblies 306 and the longitudinal buffer assemblies 305 are matched with each other to achieve multidirectional buffer effects, and the transverse buffer assemblies 306 and the longitudinal buffer assemblies 305 all provide buffer forces by utilizing mutual repulsive forces of the magnetic blocks, the subassembly of mutual friction is less, and the resistance is littleer, and it is more sensitive to absorb the vibration, provides better buffering effect, and the structure is simple relatively, and the life-span is longer, and gasbag 315 can provide the buffering of vertical direction simultaneously to further improve buffering effect.

The working process is as follows: when the culture dish is used, a culture dish cultured with cells is placed in the placing box 316, the box cover 317 is buckled on the placing box 316, then the box cover 2 is closed, the box cover 2 and the box body 1 are locked through the lock catch 5, the button is pressed, the air circulation component 4 and the temperature control component 27 are opened, and the temperature, the gas environment and the illumination intensity in the box body 1 are set according to requirements; when light supplement is needed, the main control panel 405 is set through the buttons, the lighting time and the illumination intensity of the light supplement lamp 18 are set, so as to facilitate cell culture, the oxygen sensor 10 and the carbon dioxide sensor 9 can respectively monitor the oxygen and carbon dioxide gas concentrations of the gas in the box body 1, when a certain gas concentration is lower than a set value, the main control panel 405 controls the electromagnetic valve at the bottle mouth of the corresponding gas bottle to be opened, so that the compressed gas in the gas bottle is released to the gas inlet of the circulating fan 404, and the gas is discharged into the box body 1 under the action of the circulating fan 404, so that the box body 1 is in a stable gas environment, meanwhile, the main control panel 405 controls the semiconductor refrigeration component 19 to work, the semiconductor refrigeration component 19 cools the liquid in the liquid storage box 16, so as to cool the liquid in the liquid storage box 16, and the liquid in the liquid storage box 16 is pumped into the heat exchanger 23 and is input into the heat exchange tube 13 under the action, when cold liquid circularly flows in the heat exchange tube 13, the heat in the box body 1 is absorbed, so that the temperature in the box body 1 is reduced, meanwhile, the first temperature sensor 8 monitors the temperature in the box body 1, so that the temperature in the box body 1 is constant within a certain range, when the semiconductor refrigeration assembly 19 cannot work, the main control board 405 controls the electromagnetic exhaust valve 25 to be opened, liquefied gas in the storage tank 22 is discharged and filled into a gas inlet of the heat exchanger 23, and the liquefied gas absorbs a large amount of heat during gasification, so that the liquid passing through the heat exchanger 23 is absorbed, and the temperature of the liquid can be reduced; when the temperature in the box body 1 is too low, the electric heating rod 28 is started to heat the water in the liquid storage tank 16 to a certain temperature, so that heat is released into the box body 1 under the circulation of the liquid pump 24, and the temperature in the box body 1 is raised; the circulating fan 404 of the air circulating assembly 4 sucks air in the box body 1 through the air pipe 402 and the upper exhaust plate 11 communicated with the air pipe 402, and discharges the air into the box body 1 again through the lower exhaust plate 12, when the air passes through the air pipe 402, the ultraviolet lamp 403 in the air pipe 402 irradiates the air to sterilize the air, and the circulating fan 404 continuously works, so that the air in the box body 1 is continuously sterilized, and the relatively sterile environment in the box body 1 is ensured;

when the box body 1 inclines, inclines or turns over in any direction, due to the mutual matching of the outer support ring 301, the middle support ring 302 and the inner support ring 303, when the box body 1 rotates, the fixed cylinder 307 and the upper component can keep horizontal under the action of gravity and cannot rotate along with the rotation of the box body 1, and weights such as the motor 309 and the first battery pack 310 are arranged in the fixed cylinder 307, and the weights are positioned below the axis of the movable shaft 304, so that the gravity center can be reduced, the stability of the fixed cylinder 307 is ensured, thereby avoiding the turning over and the inclination of the culture dish, when the box body 1 falls and other conditions occur, and the acceleration sensor 312 monitors larger acceleration, the control panel 311 controls the motor 309 to rotate, the motor 309 drives the rotor 313 to rotate, the rotor 313 rotates at high speed and has certain rotational inertia, so that the fixed cylinder 307 has higher stability, and ensures that the culture dish is in horizontal, meanwhile, when the box body 1 is impacted, the longitudinal buffer assemblies 305 and the transverse buffer assemblies 306 and the air bags 315 provide buffer,

it should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications are possible which remain within the scope of the appended claims.

Claims

1. The cell culture transfer heat preservation device comprises a box body (1), a box cover (2), a lock catch (5) and a heat preservation layer (14) arranged in the box body (1) and the box cover (2), it is characterized in that the bottom of the side surface of the box cover (2) is hinged with the upper part of the side surface of the box body (1) through a hinge, the lock catch (5) is positioned on the front surface of the box body (1), the self-balancing component (3) is movably arranged in the box body (1), the top of the box cover (2) is provided with an air circulation component (4), the bottom of the box body (1) is provided with a temperature control component (27), the side surface of the inner wall of the box body (1) is respectively provided with a first temperature sensor (8), a carbon dioxide sensor (9) and an oxygen sensor (10), the first temperature sensor (8), the carbon dioxide sensor (9) and the oxygen sensor (10) are electrically connected with the air circulation component (4) through leads.

2. The cell culturing, transferring and insulating device according to claim 1, wherein the air circulation assembly (4) comprises an air pipe (402), a circulating fan (404), a main control board (405) and a protective cover (401), the air pipe (402), the circulating fan (404) and the main control board (405) are all disposed in the protective cover (401), the second end of the air pipe (402) is communicated with the air inlet of the circulating fan (404), the inner side wall of the air pipe (402) is provided with an ultraviolet lamp (403), the first end of the air pipe (402) is communicated with an upper air exhaust board (11), the air outlet of the circulating fan (404) is communicated with a lower air exhaust board (12) through a pipeline, the side surface of the protective cover (401) is fixedly connected with an oxygen cylinder (406) and a carbon dioxide cylinder (407) through a fixing seat respectively, and the mouths of the oxygen cylinder (406) and the carbon dioxide cylinder (407) are communicated with electromagnetic valves, the oxygen cylinder (406) and the carbon dioxide cylinder (407) are respectively communicated with an air inlet of the circulating fan (404) through electromagnetic valves, the circulating fan (404), the ultraviolet lamp (403), the first temperature sensor (8), the carbon dioxide sensor (9) and the oxygen sensor (10) are electrically connected with the main control board (405) through wires, and the electromagnetic valve communicated with the opening of the oxygen cylinder (406) and the electromagnetic valve communicated with the opening of the carbon dioxide cylinder (407) are electrically connected with the main control board (405) through wires; the bottom of safety cover (401) and the top fixed connection of case lid (2), equal fixed connection in the top of case lid (2) is in tuber pipe (402), circulating fan (404) and main control board (405).

3. The cell culturing, transferring and incubating device according to claim 2, wherein the upper air draft plate (11) and the lower air draft plate (12) have the same structure, the upper air draft plate (11) comprises a shell (111), a plurality of air holes (112) are uniformly formed in the bottom of the shell (111), the top of the shell (111) is communicated with an air pipe (113), the air pipe (113) of the upper air draft plate (11) is communicated with the first end of the air pipe (402), the air pipe (113) of the lower exhaust plate (12) is communicated with the air outlet of the circulating fan (404), the two sides of the lower exhaust plate (12) are fixedly connected with the two sides of the inner wall of the box body (1), the top of the upper air draft plate (11) is fixedly connected with the top of the inner wall of the box body (1), and the air holes (112) of the upper air draft plate (11) are opposite to the air holes (112) of the lower air draft plate (12).

4. The cell culturing, transferring and heat preserving device according to claim 1, wherein the self-balancing assembly (3) comprises an outer support ring (301), a middle support ring (302) and an inner support ring (303), the diameters of the outer support ring (301), the middle support ring (302) and the inner support ring (303) are sequentially reduced and coaxially arranged on the same plane, the left and right sides of the outer wall of the outer support ring (301) and the front and back sides of the inner wall thereof are respectively provided with a movable shaft (304), the movable shafts (304) on the left and right sides of the outer wall of the outer support ring (301) and the movable shafts (304) on the front and back sides of the inner wall thereof are mutually perpendicular, the inner wall of the outer support ring (301) is movably connected with the outer wall of the middle support ring (302) through the two movable shafts (304), the left and right sides of the inner wall of the middle support ring (302) are also provided with movable shafts (304), and the movable shafts (304) on the left and right sides of the inner wall of the middle support ring (302) and the outer support ring (301) and the left and right sides of the outer support ring (301) The movable shaft (304) of the middle support ring (302) is coaxial, the inner wall of the middle support ring (302) is movably connected with an inner support ring (303) through the movable shaft (304), the front side and the rear side of the inner wall of the inner support ring (303) are movably connected with longitudinal buffer assemblies (305), the end parts of the two longitudinal buffer assemblies (305) are vertically and fixedly connected with transverse buffer assemblies (306), and the opposite surfaces of the two transverse buffer assemblies (306) are fixedly connected with a fixed cylinder (307);

a motor (309) is arranged in the middle of the inside of the fixed cylinder (307), an output shaft of the motor (309) is in transmission connection with a rotor (313), the upper end and the lower end of the motor (309) are fixedly connected with the side face of the inner wall of the fixed cylinder (307) through a fixed frame (308), the middle of the fixed frame (308) is fixedly connected with a first battery pack (310), a control panel (311) is arranged at the bottom of the inner wall of the fixed cylinder (307), an acceleration sensor (312) is arranged at the bottom of the fixed cylinder (307), the first battery pack (310) is electrically connected with the control panel (311) through a lead, the control panel (311) is respectively electrically connected with the acceleration sensor (312) and the motor (309) through leads, a bracket (314) is fixedly connected to the top of the fixed cylinder (307), and a placing box (316) is movably connected to the top of the bracket (314), an air bag (315) is arranged between the bottom of the placing box (316) and the top of the bracket (314), a box cover (317) is buckled at the top of the placing box (316), and a through hole is formed in the top of the box cover (317); the movable shafts (304) on the two sides of the outer wall of the outer support ring (301) are respectively movably connected with the two sides of the inner wall of the box body (1).

5. The cell culturing, trans-incubation device of claim 4, wherein the longitudinal buffer assembly (305) comprises a second support block (324), a second piston rod (323), and a sleeve (325), one end of the second piston rod (323) is connected in the sleeve (325) in a sliding way, the other end of the second piston rod (323) is fixedly connected with one end of the second supporting block (324), the end part of the piston rod and the top part of the inner wall of the sleeve (325) are fixedly connected with second magnetic blocks (326), the two second magnetic blocks (326) are mutually exclusive, bearing holes are respectively arranged at the top of the sleeve (325) and the bottom of the second supporting block (324), a bearing is arranged in each bearing hole, a rotating shaft is movably arranged in each bearing in a penetrating way, an air hole (327) is formed in the side wall of the sleeve (325), and an exhaust assembly (322) is arranged on the side surface of the air hole (327); the rotating shaft at the bottom of the second supporting block (324) is fixedly connected with the inner wall of the inner supporting ring (303);

the transverse buffer assembly (306) comprises a connecting block (318), first piston rods (319) fixedly connected with two ends of the connecting block (318) respectively and first supporting blocks (320) slidably connected with the two first piston rods (319) respectively, the end parts of the first piston rods (319) are inserted into piston holes in the side faces of the first supporting blocks (320), first magnetic blocks (321) are fixedly connected to the end parts of the two first piston rods (319) and the bottoms of the piston holes of the two fixing blocks respectively, the first magnetic blocks (321) at the end parts of the piston rods and the first magnetic blocks (321) at the bottoms of the piston holes of the fixing blocks are mutually exclusive, air holes (327) are formed in the side walls of the first supporting blocks (320), and exhaust assemblies (322) are arranged at the positions of the air holes (327); the rotating shaft at the bottom of the sleeve (325) of the longitudinal buffer assembly (305) is vertically and fixedly connected with the side wall of the connecting block (318), and the side wall of the first supporting block (320) is fixedly connected with the outer side wall of the fixed cylinder (307).

6. The cell culturing, transferring and heat preserving device of claim 5, wherein the exhaust assembly (322) comprises a soft plate (328), a through hole communicated with the air hole (327) is formed in the middle of the soft plate (328), an inner filter screen (329) is arranged in the through hole, an outer filter screen (330) is arranged on the outer side of the soft plate (328), the side wall of the outer filter screen is fixedly connected with the outer wall of the sleeve (325), and the upper part of the soft plate (328) is fixedly connected with the side wall of the sleeve (325).

7. The cell culturing, transferring and heat preserving device according to claim 1, wherein the temperature control assembly (27) comprises a liquid storage tank (16), a heat exchanging pipe (13) communicated with the upper part of the liquid storage tank (16), a liquid pump (24) communicated with the lower part of the liquid storage tank (16), a semiconductor refrigerating assembly (19) and a heat exchanger (23) closely attached to the bottom of the liquid storage tank, the second end of the heat exchanging pipe (13) is in fluid communication with the liquid outlet of the heat exchanger (23), the liquid outlet of the liquid pump (24) is in fluid communication with the liquid inlet of the heat exchanger (23), the bottom of the liquid storage tank (16) is fixedly connected with a fixing plate (21), a liquefied gas storage tank (22) is arranged on the side surface of the fixing plate (21), the storage tank (22) is stored therein, and the gas outlet of the storage tank (22) is communicated with the gas inlet of the heat exchanger (23) through two, an electromagnetic exhaust valve (25) is arranged in the middle of one pipeline communicated with the storage tank (22), a manual valve (26) is arranged in the middle of the other pipeline, and an electric heating rod (28) and a second temperature sensor (17) are arranged in the liquid storage tank (16); the two sides of the liquid storage tank (16) are fixedly connected with the two sides of the inner wall of the tank body (1) respectively, the surface of the liquid storage tank (16) is provided with a heat insulation layer (14), and the heat exchange tube (13) is spirally coiled on the side surface of the inner wall of the tank body (1).

8. The cell culturing, transferring and heat preserving device according to claim 1, wherein a second battery pack (29) is disposed at the bottom of the inner wall of the box body (1), the second battery pack (29), the liquid pump (24), the electromagnetic exhaust valve (25), the second temperature sensor (17), the electric heating rod (28) and the semiconductor refrigerating assembly (19) are electrically connected with the main control board (405) of the air circulating assembly (4) through wires respectively, the air holes (20) are disposed at the lower portion of the side wall of the box body (2), the light supplementing lamp (18) and the exhaust valve (7) are disposed at the side of the inner wall of the box cover (2), the display screen and the control button are disposed at the top of the box cover (2), the display screen, the control button and the light supplementing lamp (18) are electrically connected with the main control board (405) of the air circulating assembly (4) through wires, the transparent observation window (6) is disposed at the top of the box cover (2), a cover is arranged at the top of the transparent observation window (6); supporting legs (15) are arranged at four corners of the bottom of the box body (1).