CN113396896A - Intelligent stem cell freezing bin - Google Patents

Abstract

The invention relates to an intelligent stem cell freezing bin which comprises a control room for placing a liquid nitrogen tank, an air compressor, a compressed air buffer tank and a control cabinet; a storage chamber for placing the lifting device, the sorting device, the moving device, the shallow cooling bin, the deep cooling bin and the transition bin; a control device is arranged in the control cabinet; a shallow cooling storage device and a deep cooling storage device are respectively arranged in the shallow cooling bin and the deep cooling bin, and are provided with a plurality of layers, and stem cell storage bottles are stored by the storage devices; the shallow cold storage device is internally provided with an electric refrigeration device which is electrically connected with the control device, and the deep cold bin is internally provided with a vaporization device which is communicated with the liquid nitrogen tank. The stem cell intelligent freezing bin can automatically take, place and store stem cells; and the temperature is reduced step by step, and the energy consumption is reduced.

Description

Intelligent stem cell freezing bin

Technical Field

The invention relates to the technical field of medical instruments, in particular to an intelligent stem cell freezing bin.

Background

The stem cell is a pluripotent cell with self-renewal capacity, can be differentiated into various APSC pluripotent cells under certain conditions, and is a primitive cell with self-replication and multidirectional differentiation potential; that is, stem cells can maintain an unoriented differentiation state and have a proliferation ability, and under appropriate conditions or given appropriate signals, it can differentiate into various functional cells or tissues and organs, and is known as "universal cells" in the medical field. A stem cell, which is an unlimited or immortal self-renewing cell capable of producing at least one type of highly differentiated progeny cell.

In recent years, stem cells are defined in different levels by continuously correcting their definitions. Biologists and physicians widely believe that stem cells are cells which come from embryos, fetal tissues or adult tissues and have unlimited self-renewal, proliferation and differentiation capabilities under certain conditions, can generate daughter cells with completely the same phenotype and genotype as the stem cells, can also generate specialized cells which form body tissues and organs, and can also differentiate into progenitor cells; has the characteristic of multipotential differentiation.

After the stem cells are separated and cultured from different human tissues, the stem cells are detected and identified, and then the stem cells are frozen at the deep low temperature of-196 ℃, so that the stem cells can be revived for reinfusion of patients when clinically needed, and the purpose of treating diseases is achieved.

At present, liquid nitrogen freezing storage is the only method for storing stem cells, however, the following defects still exist in the conventional liquid nitrogen storage:

1. the stem cells need to be taken and placed in the storage tank manually, so that the danger of frostbite exists, and the sanitation level is low;

2. only liquid nitrogen is adopted for refrigeration, so that the energy consumption is large.

Disclosure of Invention

In order to solve the technical problems in the background technology, the invention provides an intelligent stem cell freezing bin which can automatically take, place and store stem cells; and the temperature is reduced step by step, and the energy consumption is reduced.

The technical scheme adopted by the invention is as follows:

freezing storehouse of stem cell intelligence includes: the air compressor control system comprises a control room and a storage room, wherein a liquid nitrogen tank, an air compressor, a compressed air buffer tank and a control cabinet are arranged in the control room, a control device is arranged in the control cabinet, and the control room is provided with a first door communicated with the outside; the storage chamber is set as a heat preservation chamber and is provided with a second door communicated with the outside.

Be provided with elevating gear, sorting device, mobile device, shallow cold storehouse, deep cold storehouse, excessive storehouse in the apotheca, be provided with shallow cold storage device, cryrogenic storage device in shallow cold storehouse, the cryrogenic storehouse respectively, shallow cold storage device, cryrogenic storage device are used for placing stem cell storage bottle.

A placing window is arranged on one side wall of the storage chamber in a penetrating manner and used for placing or taking the stem cell storage bottle; the lifting device is arranged right below the placing window and used for placing the stem cell storage bottle at a specified height; the sorting device is arranged behind the lifting device and is used for conveying the stem cell storage bottle to the lifting device or the moving device; the moving devices are arranged in parallel in the left-right direction and are arranged at the upper part of the rear part of the sorting device, so that the stem cell storage bottles can move up and down or back and forth among the sorting device, the shallow cooling bin, the deep cooling bin and the transition bin; the shallow cooling bin is arranged at the front part right below the moving device, and electric refrigeration equipment electrically connected with the control device is arranged in the shallow cooling bin and used for carrying out low-temperature preservation on stem cells in the stem cell storage bottle; the cryogenic bin is arranged at the rear part right below the moving device, and a vaporization device communicated with the liquid nitrogen tank is arranged in the cryogenic bin and is used for carrying out ultralow-temperature preservation on stem cells in the stem cell storage bottle; excessive storehouse sets up in the middle part under the mobile device, and sets up side by side between shallow cold storehouse, cryrogenic storehouse for make stem cell storage bottle cushion in cryopreservation, ultralow temperature preservation transformation process.

Further, the placement window includes:

the window body extends into the storage chamber, the open end of the window body is hinged with a window, the center of the inner bottom of the window body is convexly provided with a guide rail, and the tail end of the guide rail of the inner bottom of the window body is provided with a through hole in a penetrating manner;

a control screen electrically connected with the control device is fixedly arranged on one side of the placing window on the outer wall of the storage chamber.

Further, the lifting device includes:

the base, the base upper end is provided with slide bar, lead screw along upper and lower direction, the slide bar is provided with many, slide bar, lead screw upper end be provided with the coaxial thimble of through-hole, low side fixed connection under thimble and the window form, the lower extreme coaxial coupling of lead screw be provided with base fixed connection's servo motor, servo motor and controlling means electrical connection, the activity is provided with the slide on slide bar, the lead screw, the extrusion is provided with the placing head that inserts the through-hole on the slide, places the fixed cylinder that is provided with on the slide in first the place ahead, the stroke end of cylinder sets up forward to fixed connection is provided with "U" type push pedal, the open end inboard of push pedal is provided with the arch.

Further, the sorting device comprises:

the device comprises a base arranged on the ground of a storage chamber, wherein a first translation belt is arranged at the upper end of the base in a clearance mode, a first motor electrically connected with a control device is coaxially arranged at a rotating shaft of the first translation belt, a first limiting plate is arranged at the upper end of the first translation belt in a clearance mode, a first transition plate protruding forwards is arranged in front of the left side of the first limiting plate, a first positioning groove is arranged behind the right side of the first limiting plate, and a first channel in smooth transition with the first transition plate and the first positioning groove respectively is arranged on the first limiting plate in a penetrating mode;

a first partition plate is arranged at the upper end of the first partition plate, a second translation belt is arranged at the upper end of the first partition plate in a clearance mode, a second motor electrically connected with the control device is coaxially arranged at a rotating shaft of the second translation belt, a second partition plate is arranged at the upper end of the second translation belt in a clearance mode, a second transition plate protruding forwards is arranged in front of the left side of the second partition plate, a first positioning groove is arranged in the rear of the right side of the second partition plate, a second channel which is in smooth transition with the second transition plate and the second positioning groove respectively penetrates through the second partition plate, and a first through hole located right above the first positioning groove penetrates through the first partition plate;

a second partition plate is arranged at the upper end of the second limiting plate, a third translation belt is arranged at the upper end of the second partition plate in a clearance mode, a third motor electrically connected with the control device is coaxially arranged at a rotating shaft of the third translation belt, a third limiting plate is arranged at the upper end of the third translation belt in a clearance mode, a third transition plate protruding forwards is arranged in front of the left side of the third limiting plate, a second positioning groove is arranged behind the right side of the third limiting plate, a third channel which is in smooth transition with the third transition plate and the third positioning groove respectively penetrates through the third limiting plate, and a second through hole located right above the second positioning groove penetrates through the second partition plate;

and a third partition plate is arranged at the upper end of the third limiting plate, and a third through hole positioned right above the third positioning groove is arranged on the third partition plate in a penetrating manner.

Furthermore, a gap for accommodating the first transition plate, the second transition plate and the third transition plate is arranged on the sliding plate behind the placing head;

the first transition plate, the second transition plate and the third transition plate are sequentially arranged along the vertical direction.

Further, the mobile device is provided with a plurality of groups, including:

the connecting pipes are respectively arranged right above the first transition plate, the second transition plate and the third transition plate, the pipe orifices of the connecting pipes are hermetically provided with end sockets, the end sockets protrude towards one side and are provided with extension plates, the pipe orifices of the connecting pipes are fixedly provided with stroke cylinders, the extension plates are fixedly connected with the stroke ends of the stroke cylinders, and the end sockets are provided with limiting grooves and are matched with the pipe orifices of the connecting pipes;

the mobile device, still include:

be provided with its inside many slide rails that set up along the fore-and-aft direction, sliding connection is provided with the slide on the slide rail, the upper end of slide is provided with the location cylinder, the stroke end of location cylinder runs through the slide and fixes and be provided with the chuck, the outside cover of chuck is equipped with the bellows, the upper and lower both ends of bellows respectively with the lower extreme of slide, the upper portion fixed connection of chuck.

Further, shallow cold storehouse, excessive storehouse, cryrogenic storehouse are sealed setting side by side along the fore-and-aft direction, and the upper end in shallow cold storehouse, excessive storehouse, cryrogenic storehouse is provided with the space of holding and establishing the chuck to with mobile device sealing connection.

Further, the left and right sides that the chuck space was established to shallow cold storehouse content is provided with shallow cold storage device the left and right sides that the chuck space was established to deep cold storehouse content is provided with cryrogenic storage device, shallow cold storage device, cryrogenic storage device are provided with the multilayer, are provided with storage device on each layer respectively, storage device is provided with a plurality ofly along the fore-and-aft direction side by side, include:

the partition board is used for placing a storage device, the outer side, the inner side and the mounting groove of the partition board are arranged along the front-back direction, the partition board at the left side and the right side of the mounting groove is provided with a slideway, the mounting groove is internally provided with a transmission chain, a rotating shaft of the transmission chain is coaxially provided with a driving mechanism which is electrically connected with a control device, the guiding groove is internally provided with a thrust spring and is provided with a plurality of groups in parallel along the front-back direction, the partition board between the guiding grooves is provided with a storage barrel, two sides of the lower end of the storage barrel are downwards convexly provided with guide rods, the guide rods are slidably inserted in the guiding grooves and are abutted against the thrust spring, a partition plate is rotationally arranged in the storage barrel, the partition plate is provided with a plurality of accommodating grooves for accommodating stem cell storage bottles in a circular array along the central axis thereof, and the rotating shaft of the partition plate downwards penetrates through the storage barrel and is axially connected with a reduction gearbox, the reduction gearbox is connected with the upper end of the partition plate in a sliding mode and is provided with a synchronous motor electrically connected with the control device in a shaft connection mode, a jacking head protrudes towards one side of the slideway from the reduction gearbox, and a translation cylinder fixedly connected with the transmission chain is arranged on the slideway in a sliding mode.

Further, the chuck is provided as a pneumatic chuck;

the pneumatic control device is characterized in that the control device is provided with a plurality of electromagnetic valves and a plurality of positioners in an electrical connection mode, the electromagnetic valves are respectively communicated and arranged among the air cylinder, the stroke air cylinder, the chuck, the translation air cylinder and the compressed air buffer tank, and the positioners are communicated and arranged between the positioning air cylinder and the compressed air buffer tank.

Furthermore, a code scanner and a light supplement lamp which are electrically connected with the control device are fixedly arranged inside the clamping jaws of the chuck;

the first transition plate, the second transition plate and the third transition plate are made of magnetic conductive materials;

the stem cell storage bottle comprises:

the appearance establishes stem cell's tubbiness body, the lower extreme of body is provided with the bottle end, set up to magnetic material at the bottom of the bottle to respectively inhale with the excessive board magnetism of first excessive board, the excessive board of second, third mutually, the upper end of body is sealed and the spiro union is provided with the bottle lid, the top of bottle lid is provided with the identification code, the identification code sets up inside the jack catch of chuck to be located bar code collector, light filling lamp under.

The intelligent stem cell freezing bin has the advantages that:

1. the stem cell storage bottle is automatically taken, placed and stored through the control device;

2. through shallow cold storehouse, transition storehouse, cryrogenic storehouse of setting in parallel, realize the cooling step by step of stem cell, save the liquid nitrogen loss.

Drawings

In order to more clearly illustrate the embodiments of the present invention, reference will now be made in brief to the accompanying drawings, which are needed in the description, and in which embodiments of the present invention are illustrated.

FIG. 1 is a schematic diagram of the general arrangement of an intelligent freezing chamber for stem cells according to an embodiment of the present invention;

FIG. 2 is a schematic view of a placement window of an intelligent freezing chamber for stem cells according to an embodiment of the present invention;

FIG. 3 is an exploded view of a sorting device for an intelligent stem cell freezing bin according to an embodiment of the present invention;

FIG. 4 is an enlarged schematic view of a lifting device of the intelligent stem cell freezing chamber provided by the embodiment of the invention;

FIG. 5 is an enlarged schematic view I of a mobile device for providing an intelligent stem cell freezing chamber according to an embodiment of the present invention;

FIG. 6 is an enlarged schematic view of a second embodiment of the mobile device for providing an intelligent freezing chamber for stem cells;

FIG. 7 is a schematic view of the inside of a shallow cold storage device of an intelligent stem cell freezing bin according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of the inside of a cryogenic storage device that provides an intelligent freezing chamber for stem cells according to an embodiment of the present invention;

FIG. 9 is an enlarged view of the interior of a shallow cold storage device of the intelligent stem cell freezing bin according to an embodiment of the present invention;

FIG. 10 is an enlarged schematic view of the inside of a cryogenic storage device for an intelligent stem cell freezing bin according to an embodiment of the present invention;

fig. 11 is a schematic diagram of a storage bottle of the intelligent stem cell freezing bin provided by the embodiment of the invention.

In the figure:

1. a placing window 11, a window body 12, a window door 13, a guide rail 14 and a through hole,

2. a lifting device 21, a base 22, a slide rod 23, a lead screw 24, a top ring 25, a servo motor 26, a slide plate 27, a placing head 28, an air cylinder 29 and a push plate,

3. the sorting device is used for sorting the materials,

311. a base 312, a first partition plate 313, a second partition plate 314, a third partition plate,

321. a first translation band, 322, a second translation band, 323, a third translation band,

331. a first motor, 332, a second motor, 333, a third motor,

341. a first limit plate 342, a second limit plate 343, a third limit plate,

351. a first transition plate, 352, a second transition plate, 353, a third transition plate,

361. a first positioning groove 362, a second positioning groove 363, a first pressing groove,

371. a first through hole 372, a second through hole 373, a third through hole,

4. a moving device 40, a connecting pipe 41, a seal head 42, an extension plate 43, a stroke cylinder 44, a limit groove 45, a slide rail 46, a slide seat 47, a positioning cylinder 48, a chuck 49 and a corrugated pipe,

5-A, a shallow cooling bin, 5-B, a deep cooling bin, 6, a transition bin,

7-A, a shallow cold storage device, 7-B, a deep cold storage device, 70, a partition plate, 701, a mounting groove, 702, a slide way, 703, a guide groove, 71, a transmission chain, 72, a driving mechanism, 74, a storage barrel, 741, a guide rod, 75, a partition plate, 751, a containing groove, 76, a reduction gearbox, 77, a synchronous motor, 78, a top head, 79 and a translation cylinder,

80. a control room 81, a first door 82, a liquid nitrogen tank 83, an air compressor 84, a compressed air buffer tank 85 and a control cabinet,

90. the storage compartment, 91, the second door,

100. a control screen is provided on the display panel,

101. stem cell storage bottle 1011, body, 1012, bottle lid 1013, bottle bottom 1014, identification code.

Detailed Description

In order to clearly and clearly illustrate the specific implementation objects and the implementation modes of the invention, the technical scheme of the invention is completely described below, and the described examples are a part of the examples of the invention, but not all the examples. All other embodiments based on the described embodiments of the invention are within the scope of the invention without making creative efforts.

The intelligent freezing chamber for stem cells, disclosed by the invention, is shown in figure 1 and comprises:

the air conditioner comprises a control room 80 and a storage room 90, wherein a liquid nitrogen tank 82, an air compressor 83, a compressed air buffer tank 84 and a control cabinet 85 are arranged in the control room 80, a control device is arranged in the control cabinet 85, and the control room 80 is provided with a first door 81 used for being communicated with the outside; the storage chamber 90 is provided as a warm-keeping chamber, and the storage chamber 90 is provided with a second door 91 to communicate with the outside.

The stem cell storage bottle is characterized in that a lifting device 2, a sorting device 3, a moving device 4, a shallow cooling bin 5-A, a deep cooling bin 5-B and an excessive bin 6 are arranged in the storage chamber 90, a shallow cooling storage device 7-A and a deep cooling storage device 7-B are respectively arranged in the shallow cooling bin 5-A and the deep cooling bin 5-B, and the shallow cooling storage device 7-A and the deep cooling storage device 7-B are used for placing a stem cell storage bottle 101.

A placing window 1 is arranged on one side wall of the storage chamber 90 in a penetrating manner, and the placing window 1 is used for placing or taking the stem cell storage bottle 101. The placement window 1, as shown in fig. 2, includes: the window body 11 extends into the storage chamber 90, the opening end of the window body 11 is hinged with a window 12, the center of the inner bottom of the window body 11 is convexly provided with a guide rail 13, and the tail end of the guide rail 13 of the inner bottom of the window body 11 is provided with a through hole 14 in a penetrating manner; a control panel 100 electrically connected to the control device is fixedly installed on the outer wall of the storage chamber 90 at the side of the placement window 1.

The lifting device 2 is arranged right below the placing window 1 and used for placing the stem cell storage bottle 101 at a specified height. As shown in fig. 4, the lifting device 2 includes: the window comprises a base 21, wherein a slide rod 22 and a lead screw 23 are arranged at the upper end of the base 21 along the vertical direction, the slide rod 22 is provided with a plurality of slide rods, a top ring 24 which shares the same central axis with the through hole 14 is arranged at the upper ends of the slide rod 22 and the lead screw 23, the top ring 24 is fixedly connected with the lower end of the window 11, a servo motor 25 which is fixedly connected with the base 21 is coaxially connected with the lower end of the lead screw 23, the servo motor 25 is electrically connected with a control device, a slide plate 26 is movably arranged on the slide rod 22 and the lead screw 23, a placing head 27 inserted into the through hole 14 is protruded on the slide plate 26, a notch for accommodating a first transition plate 351, a second transition plate 352 and a third transition plate 353 is arranged on the slide plate 26 behind the placing head 27, and the first transition plate 351, the second transition plate 352 and the third transition plate 353 are made of magnetic conductive materials and are sequentially arranged along the vertical direction; an air cylinder 28 is fixedly arranged on the sliding plate 26 in front of the placing head 27, the stroke end of the air cylinder 28 is arranged forwards, a U-shaped push plate 29 is fixedly connected and arranged, and a bulge is arranged on the inner side of the opening end of the push plate 29.

The sorting device 3 is arranged behind the lifting device 2 and used for conveying the stem cell storage bottle 101 to the lifting device 2 or the moving device 4. The sorting device 3, as shown in fig. 3, comprises:

a base 311 disposed on the ground of the storage chamber 90, wherein a first translation belt 321 is disposed at an upper end gap of the base 311, a first motor 331 electrically connected to the control device is coaxially disposed at a rotating shaft of the first translation belt 321, a first limit plate 341 is disposed at an upper end gap of the first translation belt 321, a first transition plate 351 protruding forward is disposed at a left front side of the first limit plate 341, a first positioning groove 361 is disposed at a right rear side of the first limit plate 341, and a first channel respectively in smooth transition with the first transition plate 351 and the first positioning groove 361 is penetratingly disposed on the first limit plate 341;

a first partition plate 312 is arranged at the upper end of the first partition plate 341, a second translation belt 322 is arranged at the upper end of the first partition plate 312 in a clearance manner, a second motor 332 electrically connected with the control device is coaxially arranged at the rotating shaft of the second translation belt 322, a second partition plate 342 is arranged at the upper end of the second translation belt 322 in a clearance manner, a second transition plate 352 protruding forwards is arranged at the left front side of the second partition plate 342, a first positioning groove 362 is arranged at the right rear side of the second partition plate 342, second channels respectively in smooth transition with the second transition plate 352 and the second positioning groove 362 are arranged on the second partition plate 342 in a penetrating manner, and a first through hole 371 located right above the first positioning groove 361 is arranged on the first partition plate 312 in a penetrating manner;

a second partition 313 is arranged at the upper end of the second limiting plate 342, a third translation belt 323 is arranged at the upper end of the second partition 313 in a clearance manner, a third motor 333 electrically connected with the control device is coaxially arranged at the rotating shaft of the third translation belt 323, a third limiting plate 343 is arranged at the upper end of the third translation belt 323 in a clearance manner, a third transition plate 353 protruding forwards is arranged in front of the left side of the third limiting plate 343, a second positioning groove 363 is arranged behind the right side of the third limiting plate 343, a third channel respectively in smooth transition with the third transition plate 353 and the third positioning groove 363 is arranged on the third limiting plate 343 in a penetrating manner, and a second through hole 372 positioned right above the second positioning groove 362 is arranged on the second partition 313 in a penetrating manner;

the third partition 314 is disposed at the upper end of the third limiting plate 343, and a third through hole 373 located right above the third positioning groove 363 is disposed through the third partition 314.

The moving device 4 is provided with a plurality of groups in parallel along the left-right direction and is arranged at the upper part of the rear part of the sorting device 3 so as to enable the stem cell storage bottle 101 to move up and down or back and forth among the sorting device 3, the shallow cooling bin 5-A, the deep cooling bin 5-B and the transition bin 6, and the moving device 4 is provided with a plurality of groups, as shown in fig. 5, comprising:

the connecting pipe 40 is respectively arranged right above the first transition plate 351, the second transition plate 352 and the third transition plate 353, a seal head 41 is arranged at the pipe orifice of the connecting pipe 40 in a sealing mode, an extension plate 42 is arranged on the seal head 41 in a protruding mode towards one side, a stroke cylinder 43 is fixedly arranged at the pipe orifice of the connecting pipe 40, the extension plate 42 is fixedly connected with the stroke end of the stroke cylinder 43, and a limiting groove 44 is arranged on the seal head 41 and matched with the pipe orifice of the connecting pipe 40;

the mobile device 4, as shown in fig. 6, further includes:

the device is provided with a plurality of sliding rails 45 arranged inside the device along the front-back direction, a sliding seat 46 is arranged on the sliding rails 45 in a sliding connection mode, a positioning cylinder 47 is arranged at the upper end of the sliding seat 46, the stroke end of the positioning cylinder 47 penetrates through the sliding seat 46 and is fixedly provided with a chuck 48, the chuck 48 is a pneumatic chuck, a corrugated pipe 49 is sleeved outside the pneumatic chuck, and a code scanner and a light supplementing lamp which are electrically connected with a control device are fixedly arranged inside a clamping jaw of the chuck 48; the upper and lower ends of the bellows 49 are fixedly connected to the lower end of the slider 46 and the upper portion of the chuck 48, respectively.

As shown in fig. 1, the shallow cooling chamber 5-a is arranged at the front part right below the moving device 4, and an electric refrigerating device electrically connected with the control device is arranged in the shallow cooling chamber 5-a for storing stem cells in the stem cell storage bottle 101 at a low temperature; the deep cooling bin 5-B is arranged at the rear part right below the mobile device 4, and a vaporization device communicated with the liquid nitrogen tank 82 is arranged in the deep cooling bin 5-B and is used for carrying out ultra-low temperature preservation on stem cells in the stem cell storage bottle 101; the transition bin 6 is arranged in the middle of the position right below the mobile device 4 and arranged between the shallow cooling bin 5-A and the deep cooling bin 5-B in parallel, and is used for buffering the stem cell storage bottle 101 in the low-temperature storage and ultra-low-temperature storage conversion processes.

The shallow cooling bin 5-A, the transition bin 6 and the deep cooling bin 5-B are arranged in parallel and hermetically along the front-back direction, and the upper ends of the shallow cooling bin 5-A, the transition bin 6 and the deep cooling bin 5-B are provided with a space for accommodating the chuck 48 and are hermetically connected with the moving device 4. As shown in fig. 7 and 8, shallow cold storage devices 7-a are arranged on the left and right sides of the space where the chuck 48 is arranged in the shallow cold bin 5-a, deep cold storage devices 7-B are arranged on the left and right sides of the space where the chuck 48 is arranged in the deep cold bin 5-B, the shallow cold storage devices 7-a and the deep cold storage devices 7-B are provided with multiple layers, and the storage devices are respectively arranged on the layers. As shown in fig. 9 and 10, the storage device includes:

the partition board 70 for placing the storage device, the outside and inside mounting grooves 701 of the partition board 70, the guide grooves 703, the mounting groove 701 is arranged along the front-back direction, the slide ways 702 are arranged on the partition boards 70 at the left and right sides of the mounting groove 701, the driving chain 71 is arranged in the mounting groove 701, the driving mechanism 72 electrically connected with the control device is coaxially arranged on the rotating shaft of the driving chain 71, the thrust springs are arranged in the guide grooves 703, and a plurality of groups of the thrust springs are arranged in parallel along the front-back direction, the storage barrel 74 is arranged on the partition board 70 between the guide grooves 703, the guide rods 741 are arranged on the two sides of the lower end of the storage barrel 74 in a downward protruding manner, the guide rods 741 are inserted in the guide grooves 703 in a sliding manner and are abutted against the thrust springs, the storage barrel 74 is rotatably provided with the partition plate 75, a plurality of accommodating grooves 751 for accommodating the stem cell storage bottles 101 are distributed on the partition plate 75 along the central axis in a circular array, the rotating shaft of the dividing disc 75 penetrates the storage barrel 74 downwards and is provided with a reduction gearbox 76 in a shaft connection mode, the reduction gearbox 76 is connected with the upper end of the partition plate 70 in a sliding mode and is provided with a synchronous motor 77 electrically connected with the control device in a shaft connection mode, the reduction gearbox 76 is provided with a top 78 protruding towards one side of the slideway 702, and the slideway 702 is provided with a translation cylinder 79 fixedly connected with the transmission chain 71 in a sliding mode.

The control device is provided with a plurality of electromagnetic valves and a plurality of positioners in an electrical connection mode, the electromagnetic valves are respectively communicated and arranged among the air cylinder 28, the stroke air cylinder 43, the chuck 48, the translation air cylinder 79 and the compressed air buffer tank 84, and the positioners are communicated and arranged between the positioning air cylinder 47 and the compressed air buffer tank 84.

The stem cell reservoir 101, as shown in fig. 11, includes:

the stem cell-containing bottle comprises a barrel-shaped bottle body 1011 for containing stem cells, wherein a bottle bottom 1013 is arranged at the lower end of the bottle body 1011, the bottle bottom 1013 is made of a magnetic material and is magnetically attracted with a first transition plate 351, a second transition plate 352 and a third transition plate 353 respectively, a bottle cap 1012 is arranged at the upper end of the bottle body 1011 in a sealing and screwing manner, an identification code 1014 is arranged at the top end of the bottle cap 1012, and the identification code 1014 is arranged inside a clamping jaw of a chuck 48 and is positioned under a code scanner and a light supplement lamp.

According to the specific structure of the embodiment of the intelligent stem cell freezing bin, the following further describes the working method of the intelligent stem cell freezing bin:

the method for placing the intelligent stem cell freezing bin comprises the following steps:

an operator opens the window 12, places the stem cell storage bottle 101 for storing stem cells on the placing head 27 in the through hole 14 along the guide rail 13, then closes the window 12, and controls to store the stem cell storage bottle 101 placed in the through hole 14 through the control screen 100;

the control device controls the servo motor 25 to operate to drive the lead screw 23 to rotate, at this time, the sliding plate 26 slides downwards along the sliding rod 22, namely the stem cell storage bottle 101 on the placing head 27 arranged on the sliding plate 26 moves downwards until the placing head 27 is flush with the first transition plate 351, the second transition plate 352 or the third transition plate 353;

the control device controls the stroke end of the air cylinder 28 to push backwards, and at the same time, the push plate 29 pushes the stem cell storage bottle 101 backwards into the upper top surface of the first transition plate 351, the second transition plate 352 or the third transition plate 353 and onto the corresponding first translation belt 321, the second translation belt 322 or the third translation belt 323;

the control device controls the first motor 331, the second motor 332 or the third motor 333 to operate in a forward direction to drive the corresponding first translation belt 321, the second translation belt 322 or the third translation belt 323 to operate, so as to drive the stem cell storage bottle 101 to move backwards along the first limiting plate 341, the second limiting plate 342 or the third limiting plate 343 until the corresponding first positioning groove 361, the corresponding second positioning groove 362 or the corresponding first pressing positioning groove 363;

the control device controls the chuck 48 to move to the position right above the stem cell storage bottle 101, the serial number of the stem cell storage bottle 101 is recorded through the code scanner and the light supplement lamp, the chuck 48 grabs the stem cell storage bottle 101, the stem cell storage bottle is taken out of the first through hole 371, the second through hole 372 or the third through hole 373, the stem cell storage bottle moves to the shallow cooling bin 5-A, and then the stem cell storage bottle is transferred to the deep cooling bin 5-B through the transition bin 6;

the control device controls the driving mechanism 72 to operate to drive the transmission chain 71 to operate until the translation cylinder 79 moves to the top 78, the control device controls the stroke end of the translation cylinder 79 to push out, the storage barrel 74 is pushed out to the position below the stem cell storage bottle 101 grabbed by the chuck 48, the control device controls the synchronous motor 77 to operate to drive the partition plate 75 to operate until the accommodating groove 751 is positioned right below the stem cell storage bottle 101 grabbed by the chuck 48, the control device controls the chuck 48 to place the stem cell storage bottle 101 in the accommodating groove 751 and record the position of the stem cell storage bottle 101, and the control device controls the stroke end of the translation cylinder 79 to retract.

The taking method of the intelligent stem cell freezing bin comprises the following steps:

an operator selects a stem cell storage bottle 101 to be taken through the control screen 100, the control device judges the position of the stem cell storage bottle 101, the control device controls the chuck 48 to be positioned above the storage barrel 74 where the stem cell storage bottle 101 is positioned, the control device controls the servo motor 25 to operate, the lead screw 23 is driven to rotate, at the moment, the sliding plate 26 slides along the sliding rod 22 until the placing head 27 is flush with the first transition plate 351, the second transition plate 352 or the third transition plate 353, the control device controls the stroke end of the air cylinder 28 to be pushed backwards, and at the moment, the push plate 29 is positioned at the first transition plate 351, the second transition plate 352 or the third transition plate 353;

the control device controls the translation cylinder 79 to move to the top 78 of the storage barrel 74 where the stem cell storage bottle 101 is located, the storage barrel 74 is pushed out by the translation cylinder 79, meanwhile, the control device controls the synchronous motor 77 to operate, the cutting disc 75 is driven to operate, the stem cell storage bottle 101 to be taken out is located under the chuck 48, and the serial number of the stem cell storage bottle 101 is confirmed through the code scanner and the light supplement lamp;

the control device controls the chuck 48 to grab the stem cell storage bottle 101 to move, and the stem cell storage bottle 101 is placed in the first positioning groove 361, the second positioning groove 362 or the first pressing positioning groove 363 through the first perforation 371, the second perforation 372 or the third perforation 373, and controls the first motor 331, the second motor 332 or the third motor 333 to reversely rotate to drive the corresponding first translation belt 321, the second translation belt 322 or the third translation belt 323 to rotate, so that the stem cell storage bottle 101 is driven to move forwards along the first limiting plate 341, the second limiting plate 342 or the third limiting plate 343 until the stem cell storage bottle 101 is clamped into the push plate 29;

the control device controls the stroke end of the air cylinder 28 to retract forwards, at the moment, the push plate 29 pulls the stem cell storage bottle 101 to the placing head 27, the control device controls the servo motor 25 to operate to drive the lead screw 23 to rotate, at the moment, the sliding plate 26 slides upwards along the sliding rod 22, namely, the stem cell storage bottle 101 on the placing head 27 arranged on the sliding plate 26 moves upwards until the stem cell storage bottle 101 penetrates out of the through hole 14;

the operator opens the window 12, takes out the stem cell storage bottle 101 storing the stem cells from the through hole 14, and closes the window 12.

Based on the above, the embodiment of the intelligent freezing chamber for stem cells of the present invention is suggested, and various changes and modifications can be made by workers in the field without departing from the technical spirit of the present invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims (10)

1. Freezing storehouse of stem cell intelligence includes: the air compressor control system comprises a control room (80) and a storage room (90), wherein a liquid nitrogen tank (82), an air compressor (83), an air compression buffer tank (84) and a control cabinet (85) are arranged in the control room (80), a control device is arranged in the control cabinet (85), and the control room (80) is provided with a first door (81) communicated with the outside; the storage chamber (90) is arranged as a heat preservation chamber, and the storage chamber (90) is provided with a second door (91) communicated with the outside, and is characterized in that:

a lifting device (2), a sorting device (3), a moving device (4), a shallow cooling bin (5-A), a deep cooling bin (5-B) and a transition bin (6) are arranged in the storage chamber (90), a shallow cooling storage device (7-A) and a deep cooling storage device (7-B) are respectively arranged in the shallow cooling bin (5-A) and the deep cooling bin (5-B), the shallow cooling storage device (7-A) and the deep cooling storage device (7-B) are used for placing a stem cell storage bottle (101),

a placing window (1) penetrates through the wall on one side of the storage chamber (90), and the placing window (1) is used for placing or taking a stem cell storage bottle (101);

the lifting device (2) is arranged right below the placing window (1) and used for placing the stem cell storage bottle (101) at a specified height;

the sorting device (3) is arranged behind the lifting device (2) and is used for conveying the stem cell storage bottle (101) to the lifting device (2) or the moving device (4);

the moving devices (4) are arranged in parallel in the left-right direction and are arranged at the upper part behind the sorting device (3) so as to enable the stem cell storage bottle (101) to move up and down or back and forth among the sorting device (3), the shallow cooling bin (5-A), the deep cooling bin (5-B) and the transition bin (6);

the shallow cooling bin (5-A) is arranged at the front part right below the mobile device (4), and an electric refrigeration device electrically connected with the control device is arranged in the shallow cooling bin (5-A) and used for carrying out low-temperature preservation on stem cells in the stem cell storage bottle (101);

the deep cooling bin (5-B) is arranged at the rear part right below the moving device (4), and a vaporization device communicated with the liquid nitrogen tank (82) is arranged in the deep cooling bin (5-B) and is used for carrying out ultra-low temperature preservation on stem cells in the stem cell storage bottle (101);

transition storehouse (6) set up in the middle part under mobile device (4), and set up side by side between shallow cold storehouse (5-A), cryrogenic storehouse (5-B) for make stem cell storage bottle (101) buffer in cryopreservation, ultra-low temperature preserve the conversion process.

2. The intelligent stem cell freezing bin of claim 1, wherein:

the placement window (1) comprises:

the window body (11) extends into the storage chamber (90), the open end of the window body (11) is hinged with a window (12), the center of the inner bottom of the window body (11) is convexly provided with a guide rail (13), and the tail end of the guide rail (13) of the inner bottom of the window body (11) is provided with a through hole (14) in a penetrating way;

a control screen (100) electrically connected with the control device is fixedly arranged on one side of the placing window (1) on the outer wall of the storage chamber (90).

3. The intelligent stem cell freezing bin of claim 1, wherein:

the lifting device (2) comprises:

the window body is characterized by comprising a base (21), wherein a slide rod (22) and a lead screw (23) are arranged at the upper end of the base (21) along the up-down direction, a plurality of slide rods (22) are arranged on the slide rod (22), a top ring (24) sharing the central axis with a through hole (14) is arranged at the upper end of the lead screw (23), the top ring (24) is fixedly connected with the lower end of the window body (11), a servo motor (25) fixedly connected with the base (21) is coaxially connected with the lower end of the lead screw (23), the servo motor (25) is electrically connected with a control device, a sliding plate (26) is movably arranged on the slide rod (22) and the lead screw (23), a placing head (27) inserted into the through hole (14) protrudes out of the sliding plate (26), an air cylinder (28) is fixedly arranged on the sliding plate (26) in front of the placing head (27), the stroke end of the air cylinder (28) is forwards arranged, and a U-shaped push plate (29) is fixedly connected with the sliding plate, the inner side of the opening end of the push plate (29) is provided with a bulge.

4. The intelligent stem cell freezing bin of claim 1, wherein:

the sorting device (3) comprises:

the device comprises a base (311) arranged on the ground of a storage chamber (90), wherein a first translation belt (321) is arranged at the upper end of the base (311) in a clearance mode, a first motor (331) electrically connected with a control device is coaxially arranged at the rotating shaft of the first translation belt (321), a first limiting plate (341) is arranged at the upper end of the first translation belt (321) in a clearance mode, a first transition plate (351) protruding forwards is arranged in the left front of the first limiting plate (341), a first positioning groove (361) is arranged on the right rear side of the first limiting plate (341), and a first channel which is in smooth transition with the first transition plate (351) and the first positioning groove (361) respectively is arranged on the first limiting plate (341) in a penetrating mode;

a first partition plate (312) is arranged at the upper end of the first partition plate (341), a second translation belt (322) is arranged at the upper end of the first partition plate (312) in a clearance manner, a second motor (332) electrically connected with the control device is coaxially arranged at the rotating shaft of the second translation belt (322), a second partition plate (342) is arranged at the upper end of the second translation belt (322) in a clearance manner, a second transition plate (352) protruding forwards is arranged in the left front of the second partition plate (342), a first positioning groove (362) is arranged in the right rear of the second partition plate (342), a second channel which is in smooth transition with the second transition plate (352) and the second positioning groove (362) respectively is arranged in the second partition plate (342) in a penetrating manner, and a first through hole (371) positioned right above the first positioning groove (361) is arranged in the first partition plate (312) in a penetrating manner;

a second partition plate (313) is arranged at the upper end of the second limiting plate (342), a third translation belt (323) is arranged at the upper end of the second partition plate (313) in a clearance manner, a third motor (333) electrically connected with the control device is coaxially arranged at a rotating shaft of the third translation belt (323), a third limiting plate (343) is arranged at the upper end of the third translation belt (323) in a clearance manner, a third transition plate (353) protruding forwards is arranged in the left front of the third limiting plate (343), a second positioning groove (363) is arranged at the right rear of the third limiting plate (343), a third channel which is in smooth transition with the third transition plate (353) and the third positioning groove (363) respectively is arranged on the third limiting plate (343) in a penetrating manner, and a second through hole (372) located right above the second positioning groove (362) is arranged on the second partition plate (313) in a penetrating manner;

the upper end of the third limiting plate (343) is provided with a third partition plate (314), and a third through hole (373) located right above the third positioning groove (363) penetrates through the third partition plate (314).

5. The intelligent stem cell freezing bin of claim 4, wherein:

a notch for accommodating a first transition plate (351), a second transition plate (352) and a third transition plate (353) is formed in the sliding plate (26) behind the placing head (27);

the first transition plate (351), the second transition plate (352) and the third transition plate (353) are sequentially arranged along the vertical direction.

6. The intelligent stem cell freezing bin of claim 2, wherein:

the mobile device (4) is provided with a plurality of groups comprising:

the connecting pipe (40) is respectively arranged right above the first transition plate (351), the second transition plate (352) and the third transition plate (353), a sealing head (41) is arranged at the pipe orifice of the connecting pipe (40) in a sealing mode, an extension plate (42) is arranged on the sealing head (41) in a protruding mode towards one side, a stroke cylinder (43) is fixedly arranged at the pipe orifice of the connecting pipe (40), the extension plate (42) is fixedly connected with the stroke end of the stroke cylinder (43), and a limiting groove (44) is formed in the sealing head (41) and matched with the pipe orifice of the connecting pipe (40);

a mobile device (4), further comprising:

be provided with its inside many slide rails (45) that set up along the fore-and-aft direction, sliding connection is provided with slide (46) on slide (45), the upper end of slide (46) is provided with location cylinder (47), the stroke end of location cylinder (47) runs through slide (46) and fixes and be provided with chuck (48), chuck (48) outside cover is equipped with bellows (49), bellows (49) upper and lower both ends respectively with the lower extreme of slide (46), the upper portion fixed connection of chuck (48).

7. The intelligent stem cell freezing bin of claim 6, wherein:

shallow cold storehouse (5-A), excessive storehouse (6), cryrogenic storehouse (5-B) are sealed setting side by side along the fore-and-aft direction, and the upper end in shallow cold storehouse (5-A), excessive storehouse (6), cryrogenic storehouse (5-B) is provided with the space of holding and establishing chuck (48) to with mobile device (4) sealing connection.

8. The intelligent stem cell freezing bin of claim 7, wherein:

the left and right sides that chuck (48) space was established to shallow cold storehouse (5-A) content is provided with shallow cold storage device (7-A), the left and right sides that chuck (48) space was established to deep cold storehouse (5-B) content is provided with cryrogenic storage device (7-B), shallow cold storage device (7-A), cryrogenic storage device (7-B) are provided with the multilayer, are provided with storage device on each layer respectively, storage device is provided with a plurality ofly along the fore-and-aft direction side by side, include:

the partition board (70) is used for placing a storage device, the outer side and the inner side of the partition board (70) are provided with a mounting groove (701) and a guide groove (703), the mounting groove (701) is arranged along the front-back direction, the partition boards (70) on the left side and the right side of the mounting groove (701) are provided with slideways (702), the mounting groove (701) is internally provided with a transmission chain (71), a rotating shaft of the transmission chain (71) is coaxially provided with a driving mechanism (72) electrically connected with a control device, the guide groove (703) is internally provided with thrust springs and is provided with a plurality of groups in parallel along the front-back direction, the partition board (70) between the guide grooves (703) is provided with a storage barrel (74), two sides of the lower end of the storage barrel (74) are downwards convexly provided with guide rods (741), the guide rods (741) are slidably inserted into the guide grooves (703) and are abutted against the thrust springs, storage bucket (74) internal rotation is provided with cuts apart dish (75), it is provided with a plurality of appearance grooves (751) of placing stem cell storage bottle (101) to divide to distribute along the circular array of central axis on dish (75), and the pivot of dividing dish (75) runs through storage bucket (74) downwards and the coupling is provided with reducing gear box (76), reducing gear box (76) and space bar (70) upper end sliding connection to the coupling is provided with synchronous machine (77) with controlling means electrical connection, and reducing gear box (76) are provided with top (78) to slide (702) one side protrusion, slide and be provided with on slide (702) with driving chain (71) fixed connection's translation cylinder (79).

9. The intelligent stem cell freezing bin of claim 8, wherein:

the chuck (48) is configured as a pneumatic chuck;

the control device is electrically connected with a plurality of electromagnetic valves and a plurality of positioners, the electromagnetic valves are respectively communicated and arranged among the air cylinder (28), the stroke air cylinder (43), the chuck (48), the translation air cylinder (79) and the compressed air buffer tank (84), and the positioners are communicated and arranged between the positioning air cylinder (47) and the compressed air buffer tank (84).

10. The intelligent stem cell freezing bin of claim 1, wherein:

a code scanner and a light supplement lamp which are electrically connected with the control device are fixedly arranged inside the clamping jaws of the chuck (48);

the first transition plate (351), the second transition plate (352) and the third transition plate (353) are made of magnetic conductive materials;

the stem cell reservoir bottle (101) comprising:

the barrel-shaped bottle body (1011) of stem cells is held and set up, the lower extreme of bottle body (1011) is provided with bottle end (1013), bottle end (1013) sets up to magnetic material to inhale mutually with first excessive board (351), the excessive board of second (352), the excessive board of third (353) magnetism respectively, the upper end of bottle body (1011) is sealed and the spiro union is provided with bottle lid (1012), the top of bottle lid (1012) is provided with identification code (1014), identification code (1014) set up inside the jack catch of chuck (48) to be located scanner, light filling lamp under.

Images