CN113395582B - Transmittance-related intelligent two-dimensional video playing method and video device thereof - Google Patents

Transmittance-related intelligent two-dimensional video playing method and video device thereof Download PDF

Info

Publication number
CN113395582B
CN113395582B CN202010170382.8A CN202010170382A CN113395582B CN 113395582 B CN113395582 B CN 113395582B CN 202010170382 A CN202010170382 A CN 202010170382A CN 113395582 B CN113395582 B CN 113395582B
Authority
CN
China
Prior art keywords
dimensional video
transmittance
transmittances
playing
different
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010170382.8A
Other languages
Chinese (zh)
Other versions
CN113395582A (en
Inventor
张大庆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pinghu Laidun Optical Instrument Manufacturing Co ltd
Original Assignee
Pinghu Laidun Optical Instrument Manufacturing Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pinghu Laidun Optical Instrument Manufacturing Co ltd filed Critical Pinghu Laidun Optical Instrument Manufacturing Co ltd
Priority to CN202010170382.8A priority Critical patent/CN113395582B/en
Publication of CN113395582A publication Critical patent/CN113395582A/en
Application granted granted Critical
Publication of CN113395582B publication Critical patent/CN113395582B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N21/00Selective content distribution, e.g. interactive television or video on demand [VOD]
    • H04N21/40Client devices specifically adapted for the reception of or interaction with content, e.g. set-top-box [STB]; Operations thereof
    • H04N21/43Processing of content or additional data, e.g. demultiplexing additional data from a digital video stream; Elementary client operations, e.g. monitoring of home network or synchronising decoder's clock; Client middleware
    • H04N21/44Processing of video elementary streams, e.g. splicing a video clip retrieved from local storage with an incoming video stream, rendering scenes according to MPEG-4 scene graphs
    • H04N21/44008Processing of video elementary streams, e.g. splicing a video clip retrieved from local storage with an incoming video stream, rendering scenes according to MPEG-4 scene graphs involving operations for analysing video streams, e.g. detecting features or characteristics in the video stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458Fluorescence microscopy
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00Geometric image transformation in the plane of the image
    • G06T3/40Scaling the whole image or part thereof
    • G06T3/4038Scaling the whole image or part thereof for image mosaicing, i.e. plane images composed of plane sub-images
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

The invention discloses a transmittance-related intelligent two-dimensional video playing method and an intelligent two-dimensional video device thereof. The method comprises the following steps: connecting a plurality of images generated by a fluorescence optical microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sequenced from large to small according to the transmittances; and playing the two-dimensional video to enable an operator to quickly view the plurality of images respectively corresponding to the distribution of the plurality of different transmittances in the two-dimensional video. The invention comprises at least the following advantages: but the molecule transmittance of each material molecule in the liquid collection sample of short-term test, and the fluorescence molecular marker of collocation different colours can learn which kind of material molecule's quantity is too much or too little, and then help the doctor can the short-term judgement patient have unusually.

Description

Transmittance-related intelligent two-dimensional video playing method and video device thereof
Technical Field
The invention relates to the technical field of picture imaging, in particular to a transmittance-related intelligent two-dimensional video playing method based on a fluorescence optical microscope picture and a transmittance-related intelligent two-dimensional video device thereof.
Background
The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.
Fluorescence Light microscopy (Fluorescence Microscope) includes a Transmitted Light (Transmitted Light) source for Bright Field (BF) observation and an Incident Light (incorporated Light) Fluorescence source for Fluorescence (Fluorescence) observation. The bright field is the most basic mode of observation, and the light source of transmitted light can penetrate the specimen (usually a transparent or nearly transparent material), and is therefore commonly used to observe the organic tissue structure of a living body or the internal structure of a biological cell. Fluorescence observation is suitable for identification and localization of specific objects, e.g., fluorescent substances or antibodies bound to fluorescent substances (e.g., FITC), and can be used to target the location of specific objects within tissue.
It should be noted that the above background description is only for the sake of clarity and complete description of the technical solutions of the present invention and for the understanding of those skilled in the art. Such solutions are not considered to be known to the person skilled in the art merely because they have been set forth in the background section of the invention.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a transmittance-related intelligent two-dimensional video playing method based on a fluorescence optical microscope picture and a transmittance-related intelligent two-dimensional video device thereof, wherein the intelligent two-dimensional video device can rapidly switch different transmittances to check the textures and the overall appearance of different areas of an object to be researched.
The embodiment of the application discloses: a transmittance-related intelligent two-dimensional video playing method comprises the following steps: connecting a plurality of images generated by a fluorescence optical microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sorted from large to small according to the transmittances; and playing the two-dimensional video to allow an operator to quickly view the plurality of images respectively corresponding to the plurality of distributions of different transmittances in the two-dimensional video.
Further, the method further comprises: and identifying the two-dimensional video according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein a plurality of different substance molecules in the collected liquid sample respectively correspond to the plurality of fluorescent molecular markers with different colors.
Further, the method further comprises: calculating the molecular transmittance of the plurality of different substance molecules for each image; and displaying the molecular transmittance of the plurality of different substance molecules in the marked two-dimensional video.
Further, the method further comprises: the marked two-dimensional video is identified according to a designated transmittance range to generate a low-risk section and a high-risk section.
Further, the method further comprises: playing the low-risk section of the marked two-dimensional video at a first playing speed, and playing the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
The embodiment of the application discloses: a transmittance-dependent intelligent two-dimensional video device comprising: the video generation unit is used for connecting a plurality of images generated by a fluorescence optical microscope imaging system in series into a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sorted from large to small according to the transmittances; and a display unit, coupled to the video generation unit, for playing the two-dimensional video to allow an operator to quickly view the plurality of images corresponding to the plurality of distributions of different transmittances in the two-dimensional video.
Furthermore, the transmittance-related intelligent two-dimensional video device further comprises a marking unit, coupled to the video generation unit, for identifying the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules in the collected liquid sample respectively correspond to the plurality of fluorescent molecular markers of different colors.
Furthermore, the transmittance-dependent intelligent two-dimensional video apparatus further comprises a calculating unit, coupled to the marking unit, for calculating the molecular transmittances of the plurality of different material molecules for each image. The display unit displays the molecular transmittance of the plurality of different substance molecules in the marked two-dimensional video.
Further, the transmittance-dependent intelligent two-dimensional video apparatus further comprises an intelligent identification unit, coupled to the computing unit, for identifying the marked two-dimensional video according to a designated transmittance range to generate a low-risk section and a high-risk section.
Furthermore, the transmittance-related intelligent two-dimensional video device further comprises an intelligent play control unit, coupled to the intelligent identification unit and the display unit, for controlling the display unit to play the low-risk section of the marked two-dimensional video at a first play speed and play the high-risk section of the marked two-dimensional video at a second play speed, wherein the first play speed is greater than the second play speed.
By means of the technical scheme, the invention has the following beneficial effects: images shot under the fluorescence optical microscope are displayed on a screen after being spliced, and a two-dimensional video is generated, so that the whole appearance of a researched object under a microscopic field of view can be conveniently checked, and an interested area can be conveniently researched. In addition, the invention provides a light transmittance related intelligent two-dimensional video playing method and a light transmittance related intelligent two-dimensional video device thereof, which can automatically adjust the playing speed according to the content of the video, and play the low-risk section at a higher playing speed when detecting that the video is a low-risk section; when the two-dimensional video is detected to be a high-risk section corresponding to the designated transmittance range, the high-risk section is played at a slower playing speed. The invention can quickly detect the transmittance of each substance molecule in the liquid sample, and can know which substance molecule is too many or too few by matching with fluorescent molecular markers with different colors, thereby assisting doctors to quickly judge whether the patient has abnormity.
In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flowchart of a transmittance-dependent intelligent two-dimensional video playing method according to a first embodiment of the present invention.
Fig. 2 is a flowchart of a transmittance-dependent intelligent two-dimensional video playing method according to a second embodiment of the present invention.
Fig. 3 is a block diagram of a transmittance-dependent intelligent two-dimensional video apparatus according to a first embodiment of the invention.
Fig. 4 is a block diagram of a transmittance-dependent intelligent two-dimensional video apparatus according to a second embodiment of the invention.
Reference numbers to the above figures: s110, S120, S210, S220, S230, S240, S250 and a step; 30. 40, a light transmittance related intelligent two-dimensional video device; 310. a video generation unit; 320. 420, a display unit; 430. a marking unit; 440. a calculation unit; 450. an intelligent identification unit; 460. and a smart play control unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, in the description of the present invention, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is considered as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Transmittance is a physical term that indicates the ability of light to pass through a medium, and is the percentage of the luminous flux that passes through a transparent or translucent body as compared to the luminous flux incident upon it. When the human-irradiated light intensity I0 is constant, the greater the intensity Ia of light absorbed by the medium, the smaller the intensity It of transmitted light. The ability of light to transmit through the medium is denoted by It/I0, called transmittance, denoted by T, i.e. T = It/I0. The transmittance value is percentage and is 0-100%. It =0, t =0% if the light is totally absorbed by the medium. And if the light is totally transmitted, it = I0 and T =100%.
Generally, each substance in a microscope picture can be displayed by four parameters, wherein three color parameters of R, G and B can be used to represent different colors of the substance molecule, and the transmittance T can be used to represent the transmittance of the substance molecule. For example, red blood cells are thinner in the center and thicker on both sides, so that the center portion has a higher transmittance and looks brighter, and the two side portions have a lower transmittance. The white blood cells are nearly transparent and must be stained or stained with fluorescent molecules to be easily seen. In addition, the amount of the substance molecules in the liquid sample affects the transmittance of the liquid sample, and the transmittance is lower when the amount of the substance molecules is larger, and the transmittance is higher when the amount of the substance molecules is smaller.
Referring to fig. 1, fig. 1 is a flowchart illustrating a transmittance-dependent intelligent two-dimensional video playing method according to a first embodiment of the present invention. The method comprises the following steps:
s110: the method comprises the steps of connecting a plurality of images generated by a fluorescence optical microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sorted from large to small according to the transmittances.
S120: and playing the two-dimensional video to allow an operator to quickly watch a plurality of images respectively corresponding to the distribution of the plurality of different transmittances in the two-dimensional video.
In one possible embodiment, the fluid collection sample is a human blood collection sample, which includes at least biological biomolecules such as red blood cells, white blood cells, and platelets. Firstly, a plurality of images generated after a fluorescence optical microscope imaging system shoots a human blood collection sample are connected in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid collection sample, and the plurality of images are sequenced from large to small according to the transmittances. If the number of blood cells in the blood is small, the transmittance of the blood is high; if the number of blood cells in blood is large, the transmittance of blood is low. Therefore, when the two-dimensional video is played, an operator can watch the plurality of images respectively corresponding to the distribution of the plurality of different transmittances in the two-dimensional video too quickly, and further know whether the blood cell number of the patient is normal or not through the transmittance of blood.
Referring to fig. 2, fig. 2 is a flowchart illustrating a transmittance-dependent intelligent two-dimensional video playing method according to a second embodiment of the present invention. The method comprises the following steps:
s210: the method comprises the steps of connecting a plurality of images generated by a fluorescence optical microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sorted from large to small according to the transmittances.
S220: the two-dimensional video is identified according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein a plurality of different substance molecules in the collected liquid sample respectively correspond to the fluorescent molecular markers with different colors.
S230: calculating the molecular transmittances of the plurality of different substance molecules for each image; and displaying the molecular transmittance of the plurality of different substance molecules in the marked two-dimensional video.
S240: the two-dimensional video is identified and marked according to a designated transmittance range so as to generate a low-risk section and a high-risk section.
S250: the low-risk section marked with the two-dimensional video is played at a first playing speed, and the high-risk section marked with the two-dimensional video is played at a second playing speed, wherein the first playing speed is higher than the second playing speed.
In one possible embodiment, the red blood cells, white blood cells and platelets in the human blood sample are stained with different fluorescent substances, the red blood cells are stained blue, the white blood cells are stained green, and the platelets are stained purple, so that a plurality of images generated by the fluorescence light microscope imaging system are superimposed to give a two-dimensional video of the markers marked with fluorescent molecules of different colors.
Referring to fig. 4, in step S220, the labeling unit 430 identifies a two-dimensional video according to the blue, green and purple fluorescent molecular markers to generate a labeled two-dimensional video, wherein the labeled two-dimensional video includes the blue, green and purple fluorescent molecular markers, which respectively correspond to different substance molecules, such as red blood cells stained blue, white blood cells stained green and platelets stained purple, in the collected liquid sample. Thus, the viewer can be assisted in clearly distinguishing red blood cells stained blue, white blood cells stained green, and platelets stained purple.
In the above example, the molecular transmittances of red blood cells, white blood cells, and platelets were calculated separately for each image, and the molecular transmittances of red blood cells, white blood cells, and platelets were displayed in the labeled two-dimensional video. At this time, the doctor can easily determine which kind of abnormal blood cell count is present based on the molecular transmittance of these blood cells.
In human blood, 45% and 55% are blood cells and plasma, among which Red blood cells (Erythrocytes) account for the highest proportion, and the proportion of white blood cells, platelets and Red blood cells is about 1:30:500. in step S230, the molecular transmittances of the red blood cells, the white blood cells and the platelets can be further calculated and displayed in a two-dimensional indication video to easily determine which of the abnormal numbers of the blood cells is present.
In one possible embodiment, the playing speed of the two-dimensional video can be automatically adjusted. When the two-dimensional video is detected to be a low-risk section, the low-risk section is played at a higher playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to the designated transmittance range (for example, the transmittance is lower than a specific threshold), the high-risk section is played at a slower playing speed, so as to assist a doctor to quickly judge whether the patient is abnormal.
For example, if blood cells agglutinate, the transmittance may also change. Hemagglutinin (hemagglutinin) refers to an antibody or other substance that agglutinates red blood cells, and is found on the surface of influenza virus, 30194rash virus (and many other bacteria and viruses), and can attach to red blood cells of different animals to cause agglutination of red blood cells, which can be fatal in severe cases. If a patient undergoes hemagglutination, the molecular transmittance of the red blood cells is abnormally low. Therefore, the invention can quickly detect the transmittance of each substance molecule in the liquid collection sample, and can know the excessive or insufficient number of the substance molecules by matching with fluorescent molecular markers with different colors, thereby assisting doctors to quickly judge whether the patient has abnormality.
Referring to fig. 3, fig. 3 is a block diagram of a transmittance-dependent intelligent two-dimensional video apparatus 30 according to a first embodiment of the invention. The transmittance-dependent intelligent two-dimensional video device 30 includes a video generation unit 310 and a display unit 320. The video generating unit 310 is configured to serially connect a plurality of images generated by a fluorescence optical microscope imaging system into a two-dimensional video, wherein the plurality of images respectively correspond to a plurality of distributions of different transmittances of a liquid collection sample, and the plurality of images are sorted according to the transmittances from high to low. The display unit 320 is coupled to the video generating unit 310, and configured to play the two-dimensional video for an operator to quickly view the plurality of images in the two-dimensional video, which correspond to a plurality of distributions with different transmittances.
Referring to fig. 4, fig. 4 is a block diagram of a transmittance-dependent intelligent two-dimensional video apparatus 40 according to a second embodiment of the present invention. In the present embodiment, the transmittance-dependent intelligent two-dimensional video apparatus 40 includes a video generating unit 310, a display unit 420, a marking unit 430, a calculating unit 440, an intelligent identification unit 450, and an intelligent playback control unit 460. The marking unit 430 is coupled to the video generating unit 310, and configured to identify the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein a plurality of different substance molecules in the collected liquid sample respectively correspond to the plurality of fluorescent molecular markers of different colors. The calculating unit 440, coupled to the marking unit 430, is configured to calculate the molecular transmittances of the plurality of different material molecules for each image; at this time, the display unit 420 displays the molecular transmittances of the plurality of different substance molecules in the marked two-dimensional video. The intelligent recognition unit 450 is coupled to the computing unit 440, and is configured to recognize the marked two-dimensional video according to a designated transmittance range to generate a low risk section and a high risk section. The smart play control unit 460 is coupled to the smart identification unit 450 and the display unit 420, and configured to control the display unit 420 to play the low risk segment of the two-dimensional video at a first play speed and play the high risk segment of the two-dimensional video at a second play speed, where the first play speed is greater than the second play speed.
In other words, the transmittance-dependent intelligent two-dimensional video device 40 can automatically adjust the playing speed of the two-dimensional video. When the two-dimensional video is detected to be a low-risk section, the low-risk section is played at a higher playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to the designated transmittance range (for example, the transmittance is lower than a specific threshold), the high-risk section is played at a slower playing speed, so as to assist a doctor to quickly judge whether the patient has an abnormality.
By means of the technical scheme, the invention has the following beneficial effects: images shot under the fluorescence optical microscope are spliced and then displayed on a screen, and a two-dimensional video is generated, so that the whole appearance of a researched object in a microscopic view can be conveniently checked, and an interested area can be conveniently researched. In addition, the invention provides a transmittance-related intelligent two-dimensional video playing method and a transmittance-related intelligent two-dimensional video device thereof, which can automatically adjust the whole playing speed of a two-dimensional video, and when the video is detected to be a low-risk section, the low-risk section can be played at a higher playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to the designated transmittance range, the high-risk section is played at a slower playing speed. The invention can quickly detect the transmittance of each substance molecule in the liquid sample, and can know which substance molecule is too many or too few by matching with fluorescent molecular markers with different colors, thereby assisting doctors to quickly judge whether the patient has abnormity.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.

Claims (4)

1. A transmittance-related intelligent two-dimensional video playing method is characterized by comprising the following steps:
connecting a plurality of images generated by a fluorescence optical microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sequenced from large to small according to the transmittances;
playing the two-dimensional video to enable an operator to quickly watch the plurality of images which respectively correspond to the distribution of the plurality of different transmittances in the two-dimensional video;
identifying the two-dimensional video according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein a plurality of different substance molecules in the liquid collection sample respectively correspond to the plurality of fluorescent molecular markers with different colors;
calculating the molecular transmittances of the plurality of different substance molecules for each image;
displaying molecular transmittance of the plurality of different substance molecules in the labeled two-dimensional video;
the marked two-dimensional video is identified according to a designated transmittance range to generate a low-risk section and a high-risk section.
2. The transparency-dependent intelligent two-dimensional video playback method of claim 1, further comprising:
playing the low-risk section of the marked two-dimensional video at a first playing speed, and playing the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
3. A transparency-dependent intelligent two-dimensional video apparatus comprising:
the video generation unit is used for connecting a plurality of images generated by a fluorescence optical microscope imaging system in series into a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different transmittances of a liquid acquisition sample, and the plurality of images are sorted from large to small according to the transmittances;
a display unit, coupled to the video generating unit, for displaying the two-dimensional video for an operator to quickly view the plurality of images corresponding to the plurality of distributions of different transmittances;
a marking unit, coupled to the video generating unit, for identifying the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein a plurality of different substance molecules in the liquid collection sample respectively correspond to the plurality of fluorescent molecular markers of different colors;
a calculating unit, coupled to the marking unit, for calculating the molecular transmittances of the plurality of different material molecules for each image; and
an intelligent identification unit, coupled to the computing unit, for identifying the marked two-dimensional video according to a designated transmittance range to generate a low-risk section and a high-risk section;
wherein the display unit displays the molecular transmittance of the plurality of different substance molecules in the marked two-dimensional video.
4. A transmittance-dependent intelligent two-dimensional video apparatus according to claim 3, further comprising:
and the intelligent playing control unit is coupled with the intelligent identification unit and the display unit and used for controlling the display unit to play the low-risk section of the marked two-dimensional video at a first playing speed and play the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
CN202010170382.8A 2020-03-12 2020-03-12 Transmittance-related intelligent two-dimensional video playing method and video device thereof Active CN113395582B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010170382.8A CN113395582B (en) 2020-03-12 2020-03-12 Transmittance-related intelligent two-dimensional video playing method and video device thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010170382.8A CN113395582B (en) 2020-03-12 2020-03-12 Transmittance-related intelligent two-dimensional video playing method and video device thereof

Publications (2)

Publication Number Publication Date
CN113395582A CN113395582A (en) 2021-09-14
CN113395582B true CN113395582B (en) 2023-04-07

Family

ID=77615597

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010170382.8A Active CN113395582B (en) 2020-03-12 2020-03-12 Transmittance-related intelligent two-dimensional video playing method and video device thereof

Country Status (1)

Country Link
CN (1) CN113395582B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101076724A (en) * 2004-04-14 2007-11-21 美国医软科技公司 Liver disease diagnosis system, method and graphical user interface
WO2012025637A1 (en) * 2010-08-27 2012-03-01 Dublin City University An agglutination assay method and device
JP2017003865A (en) * 2015-06-12 2017-01-05 オリンパス株式会社 Microscope and microscope image acquisition method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060211071A1 (en) * 2004-12-14 2006-09-21 Millennium Pharmaceuticals, Inc. Device for aggregating, imaging and analyzing thrombi and a method of use
US20070027362A1 (en) * 2005-07-27 2007-02-01 Olympus Medical Systems Corp. Infrared observation system
WO2015024020A1 (en) * 2013-08-16 2015-02-19 The General Hospital Corporation Portable diffraction-based imaging and diagnostic systems and methods
JP2015103217A (en) * 2013-11-28 2015-06-04 ソニー株式会社 Image processing device and image processing method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101076724A (en) * 2004-04-14 2007-11-21 美国医软科技公司 Liver disease diagnosis system, method and graphical user interface
WO2012025637A1 (en) * 2010-08-27 2012-03-01 Dublin City University An agglutination assay method and device
JP2017003865A (en) * 2015-06-12 2017-01-05 オリンパス株式会社 Microscope and microscope image acquisition method

Also Published As

Publication number Publication date
CN113395582A (en) 2021-09-14

Similar Documents

Publication Publication Date Title
CN103267843B (en) Colloidal gold test paper card, corresponding collaurum analyser and method of testing
CN111699510A (en) Transformation of digital pathology images
US11099181B2 (en) Bead-based analysis of a sample
EP4345776A2 (en) Microscope system, projection unit, and image projection method
CN113218844A (en) Method for analyzing particles in sample to be detected
JP4881973B2 (en) Microtube reader device for blood sample analysis and method for reading microtube
Takko et al. ShapeMetrics: A userfriendly pipeline for 3D cell segmentation and spatial tissue analysis
ES2500916T3 (en) Procedure of cellular analysis of a sample by means of a virtual analysis plate
CN113395582B (en) Transmittance-related intelligent two-dimensional video playing method and video device thereof
KR102431217B1 (en) Image analysis apparatus, method and program
US20190178867A1 (en) Method for Identification of Tissue Objects in IHC Without Specific Staining
Falbo et al. Performance evaluation of a new and improved cuvette-based automated urinalysis analyzer with phase contrast microscopy
WO2012126346A1 (en) Method for rapidly determining dna content in cell nucleus
US20110300574A1 (en) Cell-counting method for blood hemolysis analysis in fragility measurement
CN103472218A (en) Colloidal gold chart reader and corresponding control method
CN113390867A (en) Intelligent two-dimensional video playing method related to viscosity and video device thereof
JP2021148793A (en) Method for determining characteristics of sample container in in-vitro diagnostics system, analyzing device, and in-vitro diagnostics system
CN113395595B (en) Intelligent two-dimensional video playing method related to density and video device thereof
CN204479588U (en) A kind of colloidal gold chart reader
Sato et al. Identification of SNA-I-positive cells as stem-like cells in an established cell line using computerized single-cell lineage tracking
Ramos et al. Demonstrating cerebral vascular networks: a comparison of methods for the teaching laboratory
Cousin et al. Monitoring activity-dependent bulk endocytosis in primary neuronal culture using large fluorescent dextrans
Diaz et al. Spectral phasor plot for hyperspectral imaging of acridine orange photophysics within the cell
RU2612007C2 (en) Method for automated blood cells analysis through leukocytes description based on optical features of nuclei structures
CN206002767U (en) Two-way fast microbiological imaging system

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant