CN113390987A

CN113390987A - Method for removing pigment from active component of pomegranate bark

Info

Publication number: CN113390987A
Application number: CN202110645957.1A
Authority: CN
Inventors: 阿吉艾克拜尔·艾萨; 孙光映
Original assignee: Xinjiang Technical Institute of Physics and Chemistry of CAS
Current assignee: Xinjiang Technical Institute of Physics and Chemistry of CAS
Priority date: 2021-06-10
Filing date: 2021-06-10
Publication date: 2021-09-14
Anticipated expiration: 2041-06-10
Also published as: CN113390987B

Abstract

The invention relates to a method for removing pigment from an effective component of pomegranate bark, which is characterized in that macromolecular pigment substances in the obtained effective component of the pomegranate bark are subjected to special dissolution-precipitation-adsorption on a reverse phase chromatogram, and methanol, acetonitrile and formic acid solvents with better pigment dissolution performance are added before the sample injection of the effective component of the pomegranate bark, so that the pigment in the effective component of the pomegranate bark is always kept in a dissolved state in the separation process on a column until the pigment is effectively adsorbed. The retention of macromolecular pigment substances in the pomegranate peel on the reversed phase chromatography is strong, and the retention of polyphenol substances with larger polarity on the reversed phase chromatography is weaker. The elution strength of the liquid chromatogram is adjusted, and macromolecular pigment and polyphenol in the pomegranate peel polyphenol extract can be effectively separated, so that the decolorization effect is achieved, the content and color of effective components of the pomegranate peel are effectively improved, and powerful support is provided for high-valued comprehensive utilization of the pomegranate peel.

Description

Method for removing pigment from active component of pomegranate bark

Technical Field

The invention relates to a method for removing pigment from an effective component of pomegranate bark.

Background

Pomegranate is a fruit with high economic effect value, and the dry peel of pomegranate is called pomegranate peel for short, and has the effects of relieving diarrhea with astringents, stopping bleeding and expelling parasites. It is commonly used for chronic diarrhea, chronic dysentery, hematochezia, rectocele, metrorrhagia, leukorrhagia, abdominal pain due to parasitic infestation. The pomegranate rind is rich in polyphenol compounds, and has strong antioxidant, anti-inflammatory and antibacterial effects. However, the pomegranate bark is rich in condensed tannins and macromolecular substances generated by the oxidative polymerization of polyphenol, and the color is dark red, so that the color of the micromolecular polyphenol substances in the pomegranate bark extract is dark, the biological activity of the pomegranate bark extract is influenced, and the quality of the pomegranate bark extract is also poor. In addition, the water solubility of the macromolecular pigment is poor, which is not beneficial to the further preparation application and popularization of the pomegranate bark polyphenol extract.

The traditional decoloring method such as an activated carbon decoloring method can cause great polyphenol loss and has poor effect; the gel chromatography or MCI resin decoloration is expensive, and the dead adsorption of the pigment and the polyphenol is serious, so that the application range of the pigment and the polyphenol in industrialization is limited. Therefore, in order to improve the comprehensive utilization added value of the pomegranate bark, it is necessary to develop a method capable of effectively removing macromolecular pigments in the pomegranate bark polyphenol extract. Reverse phase liquid chromatography is a widely used technique for separation and purification, and is generally used for separation and purification of compounds in various polarity ranges. However, reverse phase liquid chromatography typically uses a mobile phase having a relatively high water content in the separation of large polar materials such as polyphenols. High water content is not conducive to the dissolution of macromolecular pigments. Therefore, after the aqueous solution of the pomegranate bark polyphenol extract is subjected to sample loading and preliminary separation, macromolecular pigment molecules quickly precipitate on the chromatographic column and spread to the whole chromatographic column, and even flow out from the filling gaps of the chromatographic column filler along with the washing of the mobile phase, so that the macromolecular pigment molecules and the polyphenol substances are eluted together, and the separation effect cannot be achieved. Therefore, in the sample injection process, a solvent with good solubility to macromolecular pigments, such as methanol, formic acid, acetonitrile and the like, is introduced, and the sample is injected into the reversed phase chromatographic column for separation on the premise of not causing solvent effect. The pigment in a dissolved state is effectively adsorbed by the reversed phase chromatographic packing due to the high polarity, so that the pigment is retained on the chromatographic column. The polyphenols can be eluted from the mobile phase with high water content, so as to achieve the effect of separation and decoloration. The method is simple and effective, the filler can be repeatedly used, and the method has high industrial value.

Disclosure of Invention

The invention aims to provide a method for removing pigments in an effective component of pomegranate bark, which is based on the special dissolution-precipitation-adsorption of macromolecular pigments in the effective component of the pomegranate bark on a reverse phase chromatogram obtained in the preparation method of the effective component of the pomegranate bark with strong antioxidation and staphylococcus aureus inhibition activity of patent application No. 201911127266.1, and methanol, acetonitrile and formic acid solvents with good pigment dissolution performance are added before the sample injection of the effective component of the pomegranate bark to promote the separation process of the pigments in the effective component of the pomegranate bark on a column to always keep a dissolved state until the pigments are effectively adsorbed. The retention of macromolecular pigment substances in the pomegranate peel on the reversed phase chromatography is strong, and the retention of polyphenol substances with larger polarity on the reversed phase chromatography is weaker. The elution strength of the liquid chromatogram is adjusted, and macromolecular pigment and polyphenol in the pomegranate peel polyphenol extract can be effectively separated, so that the decolorization effect is achieved, the content and color of effective components of the pomegranate peel are effectively improved, and powerful support is provided for high-valued comprehensive utilization of the pomegranate peel. The method is simple and quick, has high decoloring efficiency, and provides an effective method for decoloring the pomegranate peel polyphenol extract and improving the product quality.

The method for removing the pigment of the effective component of the pomegranate rind comprises the following steps:

a. dissolving an effective component of pomegranate peel with 60.6 percent of punicalagin in 2L of water, ultrasonically dissolving, adding formic acid until the volume fraction of formic acid is 3-10 percent, or adding methanol until the volume fraction of methanol is 3-10 percent, or adding acetonitrile until the volume fraction of acetonitrile is 3-10 percent, and obtaining a solution for later use;

b. connecting a reversed phase C18 chromatographic column to a pilot-scale preparation liquid chromatograph, taking methanol-0.1% formic acid water with the volume ratio of 12:88-15:85 as a mobile phase, or acetonitrile-0.1% formic acid water with the volume ratio of 8:92-11:89 as a mobile phase, or tetrahydrofuran-0.1% formic acid water with the volume ratio of 7:93-10:90 as a mobile phase, controlling the flow rate to be 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature to be 10-35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for standby;

c. b, feeding the solution obtained in the step a into a chromatographic column which is well balanced in the step b, taking methanol-0.1% formic acid water with the volume ratio of 12:88-15:85 as a mobile phase, or acetonitrile-0.1% formic acid water with the volume ratio of 8:92-11:89 as a mobile phase, or tetrahydrofuran-0.1% formic acid water with the volume ratio of 7:93-10:90 as a mobile phase, controlling the temperature of the column to be 10-35 ℃, controlling the flow rate to be 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. washing the chromatographic column with methanol until the effluent is colorless, concentrating the obtained component containing punicalagin at 50 deg.C by rotary evaporation evaporator, freezing the concentrated solution, drying to obtain solid powder, measuring the content range of punicalagin by high performance liquid chromatography, concentrating the obtained column washing solution at 50 deg.C by rotary evaporation evaporator, freezing the concentrated solution, and drying to obtain red pigment.

The method for removing the pigment in the effective component of the pomegranate rind is based on the special dissolving-precipitating-adsorbing behavior of macromolecular pigment substances in the effective component of the pomegranate rind on a reverse phase chromatogram, and methanol, acetonitrile and formic acid solvents with good pigment dissolving performance are added before the effective component of the pomegranate rind is injected, so that the pigment in the effective component of the pomegranate rind is always kept in a dissolved state in the separation process on a column until the pigment is effectively adsorbed. The retention of macromolecular pigment substances in the pomegranate peel on the reversed phase chromatography is strong, and the retention of polyphenol substances with larger polarity on the reversed phase chromatography is weaker. The elution strength of the liquid chromatogram is adjusted, and macromolecular pigment and polyphenol in the active components of the pomegranate bark can be effectively separated, so that the decolorization effect is achieved, the content and color of the active components of the pomegranate bark are effectively improved, and powerful support is provided for high-valued comprehensive utilization of the pomegranate bark.

The method for removing the pigment of the effective component of the pomegranate bark, disclosed by the invention, comprises the steps of firstly utilizing special dissolution-precipitation-adsorption of the macromolecular pigment on a reversed-phase chromatographic column, adding a methanol solvent, a formic acid solvent and an acetonitrile solvent which have better solubility on the macromolecular pigment into a sample injection solvent, and then carrying out sample injection and separation, so that the large-polarity polyphenol substances in the pomegranate bark are effectively separated from the macromolecular pigment. The method is simple and effective, and can provide a new idea for improving the comprehensive utilization added value of the pomegranate rind.

The invention relates to a method for removing pigment from an effective component of pomegranate bark, which is used for obtaining the effective component of the pomegranate bark according to ZL201911127266.1 preparation method of the effective component of the pomegranate bark with strong antioxidation and staphylococcus aureus inhibition activity, and comprises the following steps:

a. by a high performance liquid chromatograph, taking methanol or acetonitrile as an organic phase A phase and 0.1% formic acid water as an aqueous phase B phase, adjusting the phase A and the phase B by a double pump of the liquid chromatograph until a mobile phase is a methanol-0.1% formic acid water mixture, wherein the content of the methanol is in a range of 5% -45%, or the mobile phase is an acetonitrile-0.1% formic acid water mixture, wherein the content of the acetonitrile is in a range of 5% -30%, the flow rate is controlled to be 2.4mL/min, the temperature of a column is room temperature, the column is a balanced reversed phase C18 chromatographic column, the length is 250mm, the inner diameter is 10mm, the particle size of a filler is 10 mu m, and the balance is carried out to a baseline level;

b. respectively weighing 500mg, 750mg, 1000mg and 1500mg of pomegranate bark extract macroporous resin purified products, dissolving the purified products in water to prepare a solution with the concentration of 500mg/mL, centrifuging to remove suspended precipitates in the solution, placing the solution in a refrigerator at the temperature of 4 ℃ for 15 hours, respectively injecting samples into the chromatographic column balanced in the step a, starting to take components after injection, connecting the obtained component solution into a container at a rate of one per minute, transferring the solution into a liquid phase vial to be tested, and taking 40 components in total from the interval of 0min to 40 min;

c. b, using a high performance liquid chromatograph, matching with an ultraviolet detector and an analytical reversed phase C18 chromatographic column, wherein the length is 250mm, the inner diameter is 4.6mm, the particle size of the filler is 10 mu m, the temperature of the column is room temperature, the flow rate is 1.0mL/min, the monitoring wavelength is 254nm, the sample injection volume of each component sample obtained in the step b is 5 microliter, and the peak area A of punicalagin in each sample and the total area A of all peaks are recorded₀Taking the sequence number (x) of each component as an abscissa, taking the ratio of punicalagin and pre-impurity of each component as an ordinate, and drawing an eta (%) -x graph and an alpha (%) -x graph on the same graph;

d. for a series of fractions obtained when the sample loading amount is 500mg in step b, combining fractions within 0min to 40min, concentrating at 50 ℃ to 5mL, and performing vacuum freeze drying to obtain fraction 1; or loading a series of 750mg fractions, mixing fractions for 0min-40min, concentrating at 50 deg.C to 5mL, and vacuum freeze drying to obtain fraction 2; or loading a series of components with sample amount of 1000mg, mixing the components for 0min-40min, concentrating at 50 deg.C to 5mL, and vacuum freeze drying to obtain component 3; or loading a series of components with sample amount of 1500mg, mixing the components for 0min-40min, concentrating at 50 deg.C to 5mL, and vacuum freeze drying to obtain component 4, which is effective component of pericarpium Granati with strong antioxidant and Staphylococcus aureus inhibiting activity.

Detailed Description

The present invention will be described in further detail with reference to the following examples.

In the examples, the effective components of pomegranate rind are all obtained from the preparation method of the effective components of pomegranate rind with strong antioxidant and staphylococcus aureus inhibitory activity, which is disclosed in the patent application No. 201911127266.1.

Example 1

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 3%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking methanol-0.1% formic acid water with the volume ratio range of 12:88 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking methanol-0.1% formic acid water with the volume ratio of 12:88 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 250g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 63.5%, the obtained column washing solution is concentrated by the rotary evaporator at the temperature of 50 ℃, and the obtained concentrated solution is dried by a freeze dryer to obtain 15g of red powder.

Example 2

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 10%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking methanol-0.1% formic acid water with a volume ratio of 15:85 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 20 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking methanol-0.1% formic acid water with a volume ratio of 15:85 as a mobile phase, controlling the temperature of the column at 20 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. washing the chromatographic column with methanol until the effluent is colorless, concentrating the fraction containing punicalagin at 50 deg.C by rotary evaporator, freezing the concentrated solution, drying to obtain 248g pale yellow solid powder, measuring the punicalagin content by high performance liquid chromatography to 64.5%, concentrating the column washing solution at 50 deg.C by rotary evaporator, freezing the concentrated solution, and drying to obtain 25g red powder.

Example 3

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 7%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking methanol-0.1% formic acid water with a volume ratio range of 14:86 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking methanol-0.1% formic acid water with a volume ratio of 14:86 as a mobile phase, controlling the temperature of the column at 35 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 245g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.5%, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 22g of red powder.

Example 4

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 10 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the column temperature at 10 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.0%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 15g of red pigment.

Example 5

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 6%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 20 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the column temperature at 20 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 249g of light yellow solid powder, the content of punicalagin in the solid powder is determined to be 66.0 percent by high performance liquid chromatography, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 26g of red pigment.

Example 6

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the temperature of the column at 35 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection is finished, washing the chromatographic column with methanol until the effluent is colorless, concentrating the obtained component containing punicalagin at the temperature of 50 ℃ by a rotary evaporation evaporator, freezing and drying the concentrated solution to obtain 250g of light yellow solid powder, measuring the content range of the punicalagin in the solid powder by using a high performance liquid chromatography to be 65.0%, concentrating the obtained column washing solution at the temperature of 50 ℃ by a rotary evaporator, freezing and drying the concentrated solution to obtain 22g of red pigment.

Example 7

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 4%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 15 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, injecting the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the temperature of the column at 15 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 251g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.5%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 23g of red pigment.

Example 8

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 25 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the column temperature at 25 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths at 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 248g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.0%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 23g of red pigment.

Example 9

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding formic acid until the volume fraction of formic acid is 8%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 9:91 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 9:91 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 242g of light yellow solid powder, the content of punicalagin in the solid powder is measured to be 63.5 percent by a high performance liquid chromatography, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 24g of red pigment.

Example 10

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 3%, wherein the obtained solution is used for later use;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 258g of light yellow solid powder, the content of punicalagin in the solid powder is measured by a high performance liquid chromatography to be 61.5 percent, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 7g of red powder.

Example 11

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 10%, wherein the obtained solution is used for later use;

d. after the collection, the chromatographic column is washed by methanol until the effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 249g of light yellow solid powder, the content of punicalagin in the solid powder is 63.5 percent by using high performance liquid chromatography, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, and the concentrated solution is dried by a freeze dryer to obtain 27g of red powder.

Example 12

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 7%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking methanol-0.1% formic acid water with a volume ratio of 13:87 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking methanol-0.1% formic acid water with a volume ratio of 13:87 as a mobile phase, controlling the temperature of the column at 35 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column was washed with methanol until the effluent was colorless, the obtained punicalagin-containing fraction was concentrated at 50 ℃ by a rotary evaporator, the concentrate was dried by a freeze dryer to obtain 262g of pale yellow powder, and the punicalagin content therein was 63.5% as determined by high performance liquid chromatography. The column-flushing solution was concentrated at 50 ℃ by a rotary evaporator, and the resulting concentrate was dried by a freeze dryer to give 18g in total of red powder.

Example 13

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 4%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 13 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the column temperature at 13 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 62.5%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 17g of red pigment.

Example 14

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 6%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 25 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the column temperature at 25 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 255g of light yellow solid powder, the content of punicalagin in the solid powder is measured by a high performance liquid chromatography to be 62.3%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 23g of red pigment.

Example 15

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 65.0%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 22g of red pigment.

Example 16

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 5%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the flow rate to be 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature to be 18 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the column temperature at 18 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 251g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.6%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 22g of red pigment.

Example 17

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 8%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 20 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the temperature of the column at 20 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 249g of light yellow solid powder, the content of punicalagin in the solid powder is measured by a high performance liquid chromatography to be 61.5%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 30g of red pigment.

Example 18

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding methanol until the volume fraction of methanol is 9%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 8:92 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 63.2%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 31g of red pigment.

Example 19

a. Weighing 300g of pomegranate peel effective component with punicalagin content of 60.6%, dissolving in 2L of water, ultrasonically dissolving, adding acetonitrile until acetonitrile volume fraction is 3%, and obtaining a solution for later use;

d. after the collection, the chromatographic column is washed by methanol until the effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 254g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 63.0%, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 21g of red powder.

Example 20

a. Weighing 300g of pomegranate peel effective component with punicalagin content of 60.6%, dissolving in 2L of water, ultrasonically dissolving, and adding acetonitrile until acetonitrile volume fraction is 10%, wherein the obtained solution is used;

d. after the collection, the chromatographic column is washed by methanol until the effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 239g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 65.0 percent, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, and the obtained concentrated solution is dried by a freeze dryer to obtain 20g of red powder.

Example 21

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding acetonitrile until the volume fraction of the acetonitrile is 7%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking methanol-0.1% formic acid water with a volume ratio of 13:87 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 10 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking methanol-0.1% formic acid water with a volume ratio of 13:87 as a mobile phase, controlling the column temperature at 10 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 242g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.5%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 22g of red pigment.

Example 22

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding acetonitrile until the volume fraction of the acetonitrile is 4%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 15 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the temperature of the column at 15 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 257g of light yellow solid powder, the content of punicalagin in the solid powder is determined to be 62.3 percent by high performance liquid chromatography, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 28g of red pigment.

Example 23

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, injecting the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 11:89 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 251g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.5%, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 19g of red powder.

Example 24

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding acetonitrile until the volume fraction of the acetonitrile is 6%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column to a pilot-scale preparative liquid chromatograph, taking acetonitrile-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking acetonitrile-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the temperature of the column at 35 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, frozen and dried to obtain 247g of light yellow solid powder, the content of punicalagin in the solid powder is measured by a high performance liquid chromatography to be 63.1%, and the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, frozen and dried to obtain 18g of red pigment.

Example 25

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding acetonitrile until the volume fraction of the acetonitrile is 10%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column (250mm multiplied by 150mm, 10 mu m) to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 30 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, injecting the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 7:93 as a mobile phase, controlling the temperature of the column at 30 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting wavelengths of 254nm and 366nm, and collecting a component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until the effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 64.0%, the obtained column washing liquid is concentrated by the rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 23g of red powder.

Example 26

b. connecting a reversed phase C18 chromatographic column to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 10:90 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 10 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 10:90 as a mobile phase, controlling the column temperature at 10 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 248g of light yellow solid powder, the content of punicalagin in the solid powder is measured by a high performance liquid chromatography to be 63.9 percent, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 26g of red pigment.

Example 27

a. Dissolving 300g of pomegranate peel active component with punicalagin content of 60.6% in 2L of water, ultrasonically dissolving, and adding acetonitrile until the volume fraction of the acetonitrile is 9%, wherein the obtained solution is used for later use;

b. connecting a reversed phase C18 chromatographic column to a pilot-scale preparative liquid chromatograph, taking tetrahydrofuran-0.1% formic acid water with a volume ratio of 8:92 as a mobile phase, controlling the flow rate at 600mL/min, balancing the chromatographic column for 15min, controlling the column temperature at 35 ℃, detecting chromatographic signals by an ultraviolet detector, and detecting the wavelengths of 254nm and 366nm for later use;

c. b, feeding the solution obtained in the step a into a chromatographic column well balanced in the step b, taking tetrahydrofuran-0.1% formic acid water with the volume ratio of 8:92 as a mobile phase, controlling the temperature of the column at 35 ℃ and the flow rate at 600mL/min, detecting chromatographic signals by an ultraviolet detector, detecting the wavelengths of 254nm and 366nm, and collecting the component containing punicalagin;

d. after the collection, the chromatographic column is washed by methanol until effluent liquid is colorless, the obtained component containing punicalagin is concentrated by a rotary evaporation evaporator at the temperature of 50 ℃, concentrated solution is frozen and dried to obtain 252g of light yellow solid powder, the content of punicalagin in the solid powder is measured by high performance liquid chromatography to be 63.4%, the obtained column washing liquid is concentrated by a rotary evaporator at the temperature of 50 ℃, the concentrated solution is frozen and dried to obtain 32g of red pigment.

Claims

1. A method for removing pigment from an effective component of pomegranate rind is characterized by comprising the following steps: