CN113383955A - Preparation method and application of zein-gum arabic nanoparticles driven by double-frequency ultrasonic-assisted salt solution dialysis - Google Patents
Preparation method and application of zein-gum arabic nanoparticles driven by double-frequency ultrasonic-assisted salt solution dialysis Download PDFInfo
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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Abstract
The invention discloses a preparation method and application of zein-gum arabic nanoparticles driven by double-frequency ultrasonic-assisted salt solution dialysis, belonging to the technical field of functional foods. The method comprises the following steps: dissolving zein, gum arabic and curcumin in a 1, 2-propylene glycol solution, stirring to obtain a mixed solution, filling the mixed solution into a dialysis bag, sealing, putting the dialysis bag into a material container, adding a salt solution or deionized water into the material container, and immersing the dialysis bag; then putting the mixture into an ultrasonic groove, setting water temperature and double-frequency ultrasonic assistance, and carrying out continuous ultrasonic; and (3) taking out the dialysis bag after the ultrasound is finished, putting the dialysis bag into the saline solution or the deionized water again for dialysis, and obtaining the zein composite nano particles after the dialysis is finished. The double-frequency ultrasound and the salt ions can jointly regulate and control the particle characteristics and the load characteristics of the nano particles, improve the particle characteristics of the nano particles and improve the storage stability and the encapsulation efficiency of the composite nano particles.
Description
Technical Field
The invention belongs to the technical field of functional foods, and particularly relates to a preparation method and application of zein-gum arabic nanoparticles driven by double-frequency ultrasonic-assisted salt solution dialysis.
Background
In order to obtain the ideal functional characteristics of the nanoparticles, the matrix protein in the nanoparticles is modified by using physical, chemical or enzymatic treatment methods, so that the conformation and structure of the protein are changed, and the physicochemical and functional characteristics of the protein are further changed. Generally, to improve the stability of nanoparticles and functional properties such as encapsulation and controlled release of bioactive compounds, the interaction between proteins and polysaccharides, surfactants, can be facilitated by the use of chemical cross-linking agents. In previous studies, Yang et al could increase the solubility and emulsion stability of proteins by phosphorylation. (Yang S, Dai L, Mao L, et al. Effect of sodium triphosphoration in physiology, structural, morphological and physiological characteristics of zein and collagen nanoparticles [ J]International Journal of Biological Macromolecules,2019,136: 653-. Currently salt solutions are commonly used to study water-soluble protein nanoparticles, such as Ca2+The influence on the characteristics of nanoparticles formed by pea protein, soybean protein, whey protein and wheat germ protein is also researched, sodium chloride is used as an excipient for spray drying to obtain a nano-structured lipid carrier, or sodium chloride is used as a matrix for loading a spice, and the performance of beta-lactoglobulin nanofibers is adjusted. The combination of salt ions and zein can improve the structural stability and aggregation state of the zein and promote the effective delivery of bioactive substances in the nano delivery carrier. But for the preparation of zein by salt solution inductionThere have been few studies on the polysaccharide/surfactant composite nanoparticles, particle characteristics, storage stability and loading characteristics of the nanoparticles are to be improved, and salt ions are limited to be used in a specific preparation method.
Disclosure of Invention
The invention aims to obtain zein nanoparticles with small particle size, uniform distribution, good storage stability and high loading rate by using different types and concentrations of salt solutions as dialyzates based on a double-frequency ultrasonic-assisted dialysis method, and the prepared zein nanoparticles are applied to load curcumin, so that the application of the ultrasonic-assisted dialysis method in zein nanoparticle self-assembly is expanded.
The technical scheme of the invention is as follows:
the method for preparing the nano-particles by driving zein self-assembly through dialysis of the double-frequency ultrasonic-assisted salt solution comprises the following steps:
(1) dissolving zein, gum arabic and curcumin in a 1, 2-propylene glycol solution to serve as an original stock solution, and stirring the original stock solution by using a magnetic stirrer to obtain a zein-gum arabic-curcumin mixed solution;
(2) putting the zein-gum arabic-curcumin mixed solution prepared in the step (1) into a dialysis bag, sealing the dialysis bag, putting the dialysis bag into a material container, adding salt solutions or deionized water with different concentrations into the material container, and immersing the dialysis bag;
(3) putting the material container containing the dialysis bag, the salt solution or the deionized water in the step (2) into an ultrasonic tank, keeping the water temperature in the ultrasonic tank constant, and setting double-frequency ultrasonic assistance, wherein the ultrasonic mode is continuous ultrasonic; and (3) taking out the dialysis bag after the ultrasound is finished, putting the dialysis bag into a container, adding a salt solution or deionized water with the volume equal to that in the step (2) into the container, dialyzing at room temperature, and obtaining the zein composite nano particles after the dialysis is finished.
Preferably, the mass ratio of the zein to the gum arabic in the step (1) is 1: 1, the mass ratio of the zein to the curcumin is 5: 1; the mass-volume ratio of the zein to the 1, 2-propylene glycol solution is 1 g: 100 mL; the volume concentration of the 1, 2-propylene glycol solution is 80%.
Preferably, the rotation speed of the stirring in the step (1) is 600rpm, and the stirring time is 3 h.
Preferably, the volume ratio of the mixed solution to the salt solution or the deionized water in the step (2) is 1:20(v: v).
Preferably, the dialysis bag in step (2) has the following specifications: the length is 45cm, the width is 34mm, and the molecular weight cut-off is 8000KD-140000 KD.
Preferably, the concentration of the different salt solutions in step (2) is determined by the concentration of salt ions in the salt solutions, the salt ion concentration being in the range of 2mM to 10mM, the salt ions including Na+With Ca2+。
Preferably, the Na is+The concentration range is 2mM-4 mM; the Ca2+The concentration range is 6mM-10 mM.
Preferably, the frequency of the dual-frequency ultrasound in the step (3) is 20/40kHz, which indicates that the ultrasound of 20kHz and 40kHz works simultaneously; the total ultrasonic power is 300W, namely 150W is distributed to the power of each ultrasonic frequency; the ultrasonic time is 8-10 min; the water temperature in the ultrasonic tank was constant at 25 ℃.
Preferably, the dialysis is performed at room temperature in step (3) for 10-11 h.
Compared with the prior art, the invention has the advantages and the technical effects that:
1. ca in the invention2+The molecular structure of proteins and polysaccharides can be altered by electrostatic interactions to bind to negatively charged groups, thus maintaining better particle characteristics over a wider range of salt ion concentrations. The composite nano-particles prepared by the method have the particle size range of 255-269 nm, the particle size range of 287-297 nm after being stored for 30 days, the encapsulation efficiency of 85-88 percent, and good storage stability and encapsulation efficiency.
2. Na in the invention+Zein-gum arabic-curcumin composite nanoparticles are prepared by electrostatic interaction, hydrophobic interaction and hydrogen bonding. Is low inNa in a concentration of+The nano particles are closely adhered to the surfaces of the nano particles through electrostatic attraction, so that the close crosslinking among the particles is promoted, and the internal space of the nano particles is widened, so that the nano particles with small size, uniform distribution and high encapsulation efficiency are obtained. The composite nano-particles prepared by the method have the particle size range of 249-410 nm, the particle size range of 314-454 nm after being stored for 30 days, the encapsulation efficiency of 69-89 percent, and better storage stability and encapsulation efficiency.
3. The particle characteristics and the load characteristics of the nanoparticles can be regulated and controlled together by the dual-frequency ultrasound and the salt ions, and the composite nanoparticles with good particle characteristics can be prepared by the synergistic effect of the dual-frequency ultrasound and the salt ions.
Drawings
FIG. 1 is a graph of encapsulation efficiency of nanoparticles prepared by dialysis-driven zein self-assembly with dual-frequency ultrasound-assisted salt solution;
zein-gum arabic-curcumin composite nanoparticle samples according to Ca added2+The concentrations are named as Z-G-C-Ca-2mM, Z-G-C-Ca-4mM, Z-G-C-Ca-6mM, Z-G-C-Ca-8mM and Z-G-C-Ca-10mM in sequence; samples were based on Na addition+The final concentrations are designated as Z-G-C-2Na-2mM, Z-G-C-2Na-4mM, Z-G-C-2Na-6mM, Z-G-C-2Na-8mM, and Z-G-C-2Na-10mM in this order; the control dialysate was designated as deionized water Z-G-C.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the specific examples and data. However, these examples are not intended to limit the technical solution of the present invention, and are only illustrative.
Example 1:
(1) 1g of zein, 1g of gum arabic and 0.2g of curcumin were dissolved in 100mL of 80% 1, 2-propanediol solution (v/v) as the original stock solution. The original stock solution was stirred using a magnetic stirrer (600rpm, 3h) to obtain a zein-gum arabic-curcumin mixed solution.
(2) Weighing 20mL of zein-gum arabic-curcumin mixed solution, filling into a dialysis bag which is subjected to activation treatment, has the length of 45cm, the width of 34mm and the molecular weight cutoff of 8000KD-140000KD, sealing the dialysis bag, putting into a material container, and adding 400mL of deionized water.
(3) And (3) placing the material container in an ultrasonic tank, setting the ultrasonic frequency to be 20/40kHz, setting the total ultrasonic power to be 300W (150W is distributed to each frequency), setting the ultrasonic time to be 10min, setting the ultrasonic mode to be continuous ultrasonic, carrying out constant-temperature water bath at 25 ℃, taking out the dialysis bag, adding deionized water with the volume equal to that of the step (2) again, and carrying out dialysis at room temperature for 11 h.
(4) And after the dialysis is finished, obtaining the zein composite nano particles. And (3) after a part of samples are refrigerated in a refrigerator at 4 ℃ for 30d, determining the particle size, PDI and Zeta potential, and taking another part of fresh colloidal solution to perform particle size, PDI, Zeta potential and encapsulation efficiency.
(5) Measurement of particle diameter and Zeta potential: the particle size distribution, PDI and potential of the samples were measured using an Antopa laser particle sizer. Diluting fresh zein dispersion liquid to a proper concentration by using deionized water, and shaking up for 2min to avoid multiple scattering effect of particles. All measurements were performed in triplicate at room temperature (25 ℃) and the results were analysed using the Kalliope software.
(6) Determination of encapsulation efficiency: fresh samples were centrifuged at 5000rpm for 10min to obtain a supernatant containing free curcumin, which was diluted with 80% 1, 2-propanediol solution (v/v). The absorbance of the supernatant was measured at 426nm using a UV-vis UV-Vis spectrophotometer. To determine the content of free curcumin in the supernatant, a standard curve was established for curcumin dissolved in 80% 1, 2-propanediol solution (v/v). After the concentration of the encapsulated curcumin was calculated, the encapsulation efficiency of the nanoparticle-loaded curcumin was obtained by the following formula.
The particle size of the zein-Arabic gum-curcumin composite nano-particles treated by the deionized water prepared by the method is 288.04 +/-9.08 nm, PDI is 0.15 +/-0.05, the potential is-19.55 +/-1.45 mV, the particle size of the nano-particles after being stored for 30 days is 364.05 +/-5.61 nm, the PDI is 0.21 +/-0.05, the potential is-24.58 +/-0.29 mV, and the encapsulation rate is 86.21 +/-7.88%.
Example 2: (Na)+Solutions)
Selection of Na for the salt solution dialysate in this example+And performing gradient experiment to select Na+The final concentration of (b) was in the range of 2mM (Z-G-C-2Na-2mM), 4mM (Z-G-C-2Na-4mM), 6mM (Z-G-C-2Na-6mM), 8mM (Z-G-C-2Na-8mM), 10mM (Z-G-C-2Na-10 mM).
(1) 1g of zein, 1g of gum arabic and 0.2g of curcumin were dissolved in 100mL of 80% 1, 2-propanediol solution (v/v) as the original stock solution. The original stock solution was stirred using a magnetic stirrer (600rpm, 3h) to obtain a zein-gum arabic-curcumin mixed solution.
(2) Weighing 20mL zein-Arabic gum-curcumin mixed solution, filling into a dialysis bag with activated length of 45cm, width of 34mM and molecular weight cutoff of 8000KD-140000KD, sealing the dialysis bag, placing into a material container, and adding Na with concentration of 2mM, 4mM, 6mM, 8mM and 10mM respectively and volume of 400mL+And (3) solution.
(3) Placing the material container in an ultrasonic tank, setting the ultrasonic frequency at 20/40kHz, the total ultrasonic power at 300W (150W is distributed for each frequency), the ultrasonic time at 10min, the ultrasonic mode is continuous ultrasonic, the constant temperature water bath is 25 ℃, taking out the dialysis bag, and adding Na with the same volume as that in the step (2) again+The solution was dialyzed at room temperature for 11 h.
(4) And after the dialysis is finished, obtaining the zein composite nano particles. And (3) after a part of samples are refrigerated in a refrigerator at 4 ℃ for 30d, determining the particle size, PDI and Zeta potential, and taking another part of fresh colloidal solution to perform particle size, PDI, Zeta potential and encapsulation efficiency.
(5) Measurement of particle diameter and Zeta potential: the particle size distribution, PDI and potential of the samples were measured using an Antopa laser particle sizer. Diluting fresh zein dispersion liquid to a proper concentration by using deionized water, and shaking up for 2min to avoid multiple scattering effect of particles. All measurements were performed in triplicate at room temperature (25 ℃) and the results were analysed using the Kalliope software.
(6) Determination of encapsulation efficiency: fresh samples were centrifuged at 5000rpm for 10min to obtain supernatant containing free curcumin, which was diluted with 80% propylene glycol aqueous solution (v/v). The absorbance of the supernatant was measured at 426nm using a UV-vis UV-Vis spectrophotometer. To determine the content of free curcumin in the supernatant, a standard curve was established for curcumin dissolved in 80% propylene glycol aqueous solution (v/v). After the concentration of the encapsulated curcumin was calculated, the encapsulation efficiency of the nanoparticle-loaded curcumin was obtained by the following formula.
Na with different concentrations is prepared by the method+Treated zein-gum arabic-curcumin composite nanoparticles. According to the concentration gradient, the particle diameters of 2mM, 4mM, 6mM, 8mM and 10mM are 277.68 + -12.43 nm, 249.46 + -14.93 nm, 289.89 + -3.33 nm, 367.56 + -66.09 nm and 410.63 + -6.60 nm respectively, the PDI is 0.08 + -0.06, 0.17 + -0.09, 0.24 + -0.05, 0.24 + -0.11 and 0.24 + -0.05 respectively, the potentials are-17.88 + -0.74 mV, -13.13 + -1.14 mV, -15.82 + -1.12 mV, -11.29 + -1.45 mV and-10.35 + -0.37 mV respectively, the particle diameters of nanoparticles after 30d storage are 315.46 + -18.24 nm, 316.55 + -4.60 nm, 314.00 + -16.23 nm, 432.80 + -14.66 mM, 454.75 mM, 10mM and 10mM are 315.46 + -18.24 nm, -23.27.27 + -0.27.27 mV, 0.05 + -0.27.27.27-0.27.27 mV respectively, and the potentials are-0.05 + -0.27.05 + -0.9 mV respectively. In comparison with example 1, Na concentration was low+The particle size after treatment was significantly reduced, PDI was reduced, the size distribution was uniform, and the particle size remained small during storage, indicating a low concentration of Na+The generation of uniform and stable nanoparticles is promoted, the particle characteristics of the nanoparticles are improved, and the storage stability of the composite nanoparticles is improved. And high concentration of Na+It is possible to promote the cohesive flocculation of the nanoparticles, resulting in aggregation of the composite nanoparticles and an increase in particle size during storage. 2mM, 4mM, 2mM,6mM, 8mM, 10mM Na+The encapsulation rates of the treated composite nano-particles are respectively 86.27 +/-6.44%, 89.92 +/-8.15%, 82.17 +/-6.44%, 78.25 +/-6.71%, 69.258 +/-6.89% and Na+The encapsulation efficiency of the nanoparticles prepared after the solution dialysis shows a trend of increasing and then decreasing, and the Na concentration is low+The loading rate of the composite nano-particles is improved.
Example 3: (Ca)2+Solutions)
Selection of Ca for the salt solution dialysate of this example2+And performing gradient experiment to select Ca2+The concentration of (B) is in the range of 2mM (Z-G-C-Ca-2mM), 4mM (Z-G-C-Ca-4mM), 6mM (Z-G-C-Ca-6mM), 8mM (Z-G-C-Ca-8mM), 10mM (Z-G-C-Ca-10 mM).
(1) 1g of zein, 1g of gum arabic and 0.2g of curcumin were dissolved in 100mL of 80% 1, 2-propanediol solution (v/v) as the original stock solution. The original stock solution was stirred using a magnetic stirrer (600rpm, 3h) to obtain a zein-gum arabic-curcumin mixed solution.
(2) Weighing 20mL zein-Arabic gum-curcumin mixed solution, filling into a dialysis bag with activated length of 45cm, width of 34mM and molecular weight cutoff of 8000KD-140000KD, sealing the dialysis bag, placing into a material container, and adding Ca with concentration of 2mM, 4mM, 6mM, 8mM and 10mM respectively and volume of 400mL2+And (3) solution.
(3) Placing the material container in an ultrasonic tank, setting the ultrasonic frequency at 20/40kHz, the total ultrasonic power at 300W (150W is distributed for each frequency), the ultrasonic time at 10min, the ultrasonic mode is continuous ultrasonic, the constant temperature water bath is 25 ℃, taking out the dialysis bag, and adding Ca with the same volume as that in the step (2) again2+The solution was dialyzed at room temperature for 11 h.
(4) And after the dialysis is finished, obtaining the zein composite nano particles. And (3) after a part of samples are refrigerated in a refrigerator at 4 ℃ for 30d, determining the particle size, PDI and Zeta potential, and taking another part of fresh colloidal solution to perform particle size, PDI, Zeta potential and encapsulation efficiency.
(5) Measurement of particle diameter and Zeta potential: the particle size distribution, PDI and potential of the samples were measured using an Antopa laser particle sizer. Diluting fresh zein dispersion liquid to a proper concentration by using deionized water, and shaking up for 2min to avoid multiple scattering effect of particles. All measurements were performed in triplicate at room temperature (25 ℃) and the results were analysed using the Kalliope software.
(6) Determination of encapsulation efficiency: fresh samples were centrifuged at 5000rpm for 10min to obtain supernatant containing free curcumin, which was diluted with 80% propylene glycol aqueous solution (v/v). The absorbance of the supernatant was measured at 426nm using a UV-vis UV-Vis spectrophotometer. To determine the content of free curcumin in the supernatant, a standard curve was established for curcumin dissolved in 80% propylene glycol aqueous solution (v/v). After the concentration of the encapsulated curcumin was calculated, the encapsulation efficiency of the nanoparticle-loaded curcumin was obtained by the following formula.
Ca with different concentrations is prepared by the method2+Treated zein-gum arabic-curcumin composite nanoparticles. According to the concentration gradient, the particle diameters of 2mM, 4mM, 6mM, 8mM and 10mM are 260.27 + -3.61 nm, 266.92 + -10.43 nm, 255.12 + -7.79 nm, 256.42 + -6.66 nm and 269.14 + -9.79 nm respectively, the PDI is 0.13 + -0.1, 0.13 + -0.06, 0.21 + -0.02, 0.15 + -0.06 and 0.14 + -0.05 respectively, the potentials of the nanoparticles are-21.74 + -2.29 mV, -19.79 + -1.94 mV, -19.52 + -2.36 mV, -20.85 + -5.24 mV and-22.36 + -0.63 mV respectively, the particle diameters of the nanoparticles after 30d storage are 297.42 + -6.97 nm, 287.51 + -2.54 nm, 291.24 + -10.87 nm, 2 + -7.42 mM, 23 mM, 8mM and 10mM are 297.42 + -6.97 nm, -23.23.05 + -0.05 and-23.05 + -0.05-23.05 mV respectively, and the potentials of the nanoparticles are 297.42 + -0.7.7.7.7.7.7.7 mV, 23.20.20.23 mV, 23.20.20.20.20.23 mM respectively.
Ca compares with examples 1 and 22+Good potential is shown in promoting cross-linking between the interior of the nanoparticles. Ca at different concentrations2+Under the action of horizontal and ultrasonic waves, the molecular chain of zein is partially unfolded and exposed outMultiple active sites that interact with and bind to gum arabic to form a compact and stable complex. After 30 days of storage, the salt solution induced greater stability of the composite nanoparticles than the deionized water treated nanoparticles, and even though the particle size increased after storage, the formation of stable nanoparticles was still within acceptable limits. 2mM, 4mM, 6mM, 8mM, 10mM Ca2+The encapsulation efficiency of the treated composite nano-particles is 85.16 +/-6.71%, 86.84 +/-8.6%, 86.16 +/-7.34%, 88.59 +/-8.24% and 87.63 +/-7.52% respectively. Calcium ion-regulated composite nanoparticles, wherein calcium ions promote nanoparticles to form a relatively compact structure with each other, so that the internal limited capacity is caused, and the encapsulation efficiency is highest at the concentration of 8 mM.
Table 1 shows the particle size, PDI and Zeta potential of a fresh sample of zein-gum arabic-curcumin composite nanoparticles prepared by dialysis of a double-frequency ultrasound-assisted salt solution
Table 2 shows the particle size, PDI and Zeta potential of zein-gum arabic-curcumin composite nanoparticles prepared by dialysis of double-frequency ultrasound-assisted salt solution after being stored for 30 days
The stability of the zein-gum arabic-curcumin composite nanoparticles is greatly influenced by the dialysis of the double-frequency ultrasonic-assisted salt solution. For fresh samples, low Na concentrations are shown in Table 1+The particle size of the nanoparticles can be reduced because the electrostatic repulsive force can be well protected by the salt ions, and thus the protein and polysaccharide molecules can form an aggregate with a compact structure. Higher concentration of Na+The existence of the protein will be shieldedSome negative charge on the surface, which interferes with nanoparticle formation, may form a thicker ionic coating on the nanoparticle surface. Therefore, the sudden drop of electrostatic repulsion on the surface of the nanoparticles can cause the cross-linking between different particles without maintaining stability, and the nanoparticles are randomly aggregated. And Ca2+For zein-gum arabic-curcumin composite nanoparticles, the ion concentration increase is consistently smaller for nanoparticle size than deionized water induced nanoparticles, the Zeta potential is relatively constant, which can be explained as the potential of the nanoparticles is relatively constant with Ca2+Close to the potential of the sample without salt ion induction, calcium ions show good potential in promoting cross-linking between nanoparticle interiors. After 30 days of storage, the salt solution induced stability of the composite nanoparticles was greater than that of the nanoparticles prepared with deionized water as dialysate (except for 8mM, 10mM Na concentration+) Even with increased particle size after storage, the formation of stable nanoparticles is still within acceptable ranges.
Description of the drawings: the above embodiments are only used to illustrate the present invention and do not limit the technical solutions described in the present invention; thus, while the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted; all such modifications and variations are intended to be included herein within the scope of this disclosure and the present invention and protected by the following claims.
Claims (10)
1. A preparation method of zein-gum arabic nanoparticles driven by double-frequency ultrasound-assisted salt solution dialysis is characterized by comprising the following steps:
(1) dissolving zein, gum arabic and curcumin in a 1, 2-propylene glycol solution to serve as an original stock solution, and stirring the original stock solution by using a magnetic stirrer to obtain a zein-gum arabic-curcumin mixed solution;
(2) putting the zein-gum arabic-curcumin mixed solution prepared in the step (1) into a dialysis bag, sealing the dialysis bag, putting the dialysis bag into a material container, adding salt solutions or deionized water with different concentrations into the material container, and immersing the dialysis bag;
(3) putting the material container containing the dialysis bag, the salt solution or the deionized water in the step (2) into an ultrasonic tank, keeping the water temperature in the ultrasonic tank constant, and setting double-frequency ultrasonic assistance, wherein the ultrasonic mode is continuous ultrasonic; and (3) taking out the dialysis bag after the ultrasound is finished, putting the dialysis bag into a container, adding a salt solution or deionized water with the volume equal to that in the step (2) into the container, dialyzing at room temperature, and obtaining the zein composite nano particles after the dialysis is finished.
2. The method for preparing zein-gum arabic nanoparticles by dialysis driven with double-frequency ultrasound-assisted salt solution according to claim 1, wherein the mass ratio of zein to gum arabic in step (1) is 1: 1, the mass ratio of the zein to the curcumin is 5: 1; the mass-volume ratio of the zein to the 1, 2-propylene glycol solution is 1 g: 100 mL; the volume concentration of the 1, 2-propylene glycol solution is 80%.
3. The method for preparing zein-gum arabic nanoparticles by dialysis driven with dual-frequency ultrasound-assisted salt solution as claimed in claim 1, wherein the stirring speed in step (1) is 600rpm and the stirring time is 3 h.
4. The method of claim 1, wherein the volume ratio of the mixed solution to the saline solution or the deionized water in step (2) is 1: 20.
5. The method for preparing zein-gum arabic nanoparticles by dialysis driven with dual-frequency ultrasound-assisted salt solution according to claim 1, wherein the specification of the dialysis bag in step (2) is as follows: the length is 45cm, the width is 34mm, and the molecular weight cut-off is 8000KD-140000 KD.
6. The method of claim 1, wherein the concentration of the different salt solutions in step (2) is determined by the concentration of salt ions in the salt solutions, wherein the concentration of salt ions is in the range of 2mM to 10mM, and wherein the salt ions comprise Na+With Ca2+。
7. The method of claim 6, wherein the Na is selected from the group consisting of sodium, potassium, magnesium, calcium, magnesium, and magnesium+The concentration range is 2mM-4 mM; the Ca2+The concentration range is 6mM-10 mM.
8. The method for preparing zein-gum arabic nanoparticles by dialysis driven with dual-frequency ultrasound-assisted salt solution as claimed in claim 1, wherein the dual-frequency ultrasound frequency in step (3) is 20/40kHz, indicating that 20kHz and 40kHz ultrasound work simultaneously; the total ultrasonic power is 300W, namely 150W is distributed to each frequency of power; the ultrasonic time is 8-10 min; the water temperature in the ultrasonic tank was constant at 25 ℃.
9. The method of claim 1, wherein the dialysis at room temperature in step (3) is performed for 10-11 h.
10. Use of zein-gum arabic nanoparticles prepared according to the process of any one of claims 1-9 for loading curcumin.
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