CN113358734A - Food net stability evaluation method based on stable isotope technology - Google Patents
Food net stability evaluation method based on stable isotope technology Download PDFInfo
- Publication number
- CN113358734A CN113358734A CN202110749417.8A CN202110749417A CN113358734A CN 113358734 A CN113358734 A CN 113358734A CN 202110749417 A CN202110749417 A CN 202110749417A CN 113358734 A CN113358734 A CN 113358734A
- Authority
- CN
- China
- Prior art keywords
- delta
- area
- stable isotope
- food net
- toc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013305 food Nutrition 0.000 title claims abstract description 43
- 238000011156 evaluation Methods 0.000 title claims abstract description 21
- 238000005516 engineering process Methods 0.000 title claims abstract description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 36
- 235000016709 nutrition Nutrition 0.000 claims abstract description 25
- 230000035764 nutrition Effects 0.000 claims abstract description 23
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 13
- 238000011160 research Methods 0.000 claims abstract description 12
- 230000008901 benefit Effects 0.000 claims abstract description 6
- 241000251468 Actinopterygii Species 0.000 claims description 8
- 241000238557 Decapoda Species 0.000 claims description 6
- 241000251511 Holothuroidea Species 0.000 claims description 6
- 238000011161 development Methods 0.000 claims description 6
- 210000003205 muscle Anatomy 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 241000270666 Testudines Species 0.000 claims description 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 3
- 239000012159 carrier gas Substances 0.000 claims description 3
- 238000007405 data analysis Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 3
- 230000000155 isotopic effect Effects 0.000 claims description 3
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 230000009182 swimming Effects 0.000 claims description 2
- 238000001727 in vivo Methods 0.000 claims 1
- 230000001502 supplementing effect Effects 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000037406 food intake Effects 0.000 abstract description 2
- 235000012631 food intake Nutrition 0.000 abstract description 2
- 238000003306 harvesting Methods 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 244000062645 predators Species 0.000 description 3
- 241001249555 Hexagrammos otakii Species 0.000 description 2
- 241000132114 Sebastiscus marmoratus Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 241001582957 Sebastes schlegelii Species 0.000 description 1
- 241000132116 Sebastiscus Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 230000029602 competition with other organism Effects 0.000 description 1
- 230000001808 coupling effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/11—Complex mathematical operations for solving equations, e.g. nonlinear equations, general mathematical optimization problems
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Data Mining & Analysis (AREA)
- Theoretical Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mathematical Optimization (AREA)
- Mathematical Analysis (AREA)
- Computational Mathematics (AREA)
- Operations Research (AREA)
- Algebra (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Databases & Information Systems (AREA)
- Software Systems (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention relates to the technical field of ecological evaluation, in particular to a food net stability evaluation method based on a stable isotope technology. By measuring and calculating delta in the sample13CTOCAnd delta15NTONValues then respectively delta13CTOCAnd delta15NTONThe value is horizontal and vertical coordinates, and a two-dimensional point set of the carbon and nitrogen stable isotope ratio of each population is drawn to obtain delta13Variation of C value (CR) and delta15And (3) five parameters of N value amplitude variation (NR), nutrition ecological niche area (TA), Standard Elliptical Area (SEA) and nutrition ecological niche Overlapping Area (OA), finishing the evaluation of the stability of the food net and performing manual intervention according to the evaluation result. The invention can monitor and research the nutrition ecological niche of main populations in the sea area food net in real time, evaluate the food intake competition among the populations, and provide data for accurate proliferation and releasing and fishery resource harvesting in the sea research areaThe supporting method realizes the purpose of fine ecological breeding of marine industry and improves the marine economic benefit.
Description
Technical Field
The invention relates to the technical field of ecological evaluation, in particular to a food net stability evaluation method based on a stable isotope technology.
Background
At present, the evaluation of the effect of the marine environmental ecosystem or the evaluation of the ecosystem in a restored sea area mostly takes underwater camera shooting and fishing as means to estimate the dominance, diversity, uniformity and the like of the population in the biological community, so that the efficiency is low, the investment is high, the actual effect is not ideal, and the dynamic change of the biological community in the marine ecosystem is not accurately mastered. The nutrition level relationship is the most important relationship among all the biological members in the community, is the basis for the community to live, and is the core for understanding the energy flow of the ecosystem. However, the traditional research method of the nutrition dynamics of the marine food net is a feeding analysis method, and a method for determining the basic structure and the food relation of the food net by analyzing the population composition and the quantity of the contents in the digestive tract of a predator is adopted, so that the workload is huge, and the nutrition relation among organisms with different nutrition levels is difficult to clear. The stable isotope method can estimate the coupling effect of each population in the ecological system of the marine research area, and provides data support for the natural sustainable development of the marine ecological system through accurate manual intervention, so that a 'simplified food net' method can be adopted to highlight the conversion relation between the nutrient content of the main resource species and the food quality.
Carbon and nitrogen stable isotopes are reflected in the accumulation of food ingested by predators during a significant period of life, digested by metabolism, and absorbed. Delta between consumer and food15The N difference is the nutrient fraction, with delta increasing with nutrient level15With increasing N value, between different nutrition levels15The average value of the N enrichment was 3.4 ‰. Carbon stable isotope ratio (delta) of substances of different organic origin13C) There is a certain difference, the consumer's delta13C may reflect delta of food it digests and absorbs13C, the difference between the two is 0 to 1 ‰, thus delta13C is often used to analyze the food source of consumers in ecosystems. Therefore, a theoretical basis is provided for evaluating the stability of the food net by adopting the stable isotope technology.
Disclosure of Invention
The invention aims to solve the technical problem that the traditional research method for the nutrition dynamics of the marine food net is an edible analysis method, adopts a method for analyzing the population composition and the quantity of the contents in the digestive tract of a predator and determining the basic structure and the food relation of the food net, has huge workload and is difficult to clearly learn the nutrition relation among organisms with different nutrition levels.
In order to solve the problems, the invention provides a food net stability evaluation method based on a stable isotope technology, which utilizes the stable isotope technology to monitor and research the nutritional ecological niches of main populations in a sea food net in real time, evaluates the food intake competition among the populations, provides a data support method for accurate proliferation and releasing and fishery resource harvesting in a marine research area, and manually regulates and controls the directions of material circulation and energy flow in the food net of an ecological system in the research area, thereby realizing the purpose of fine ecological breeding in marine industry and improving the marine economic benefit.
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for evaluating the stability of food net based on stable isotope technique features that the delta in specimen is measured and calculated13CTOCAnd delta15NTONValues then respectively delta13C、δ15The N value is horizontal and vertical coordinates, and a two-dimensional point set of the carbon and nitrogen stable isotope ratios of each population is drawn to obtain delta13Variation of C value (CR) and delta15And (3) five parameters of N value variation (NR), nutrition ecological niche area (TA), Standard Elliptical Area (SEA) and nutrition ecological niche Overlapping Area (OA) are adopted to finish the evaluation of the stability of the biological community food net in the research area.
The method specifically comprises the following steps:
(1) collecting and processing the main consumer samples in the area;
(2) measuring the stable isotope;
(3) estimating the nutrition ecological niche;
(4) evaluating the stability of the food net, predicting the development direction of the food net and carrying out manual intervention.
Further, the step (1) is specifically to collect samples of the swimming organisms from the sea in the research area according to GB/T12763.1-2007 Marine survey Specifications; the sample composition mainly comprises fish, shrimp, crab and sea cucumber, wherein the body muscle of the fish, shrimp and sea cucumber is taken, and the large turtle muscle of the crab is taken; the sample is dried for 48h at 60 ℃ in a drying oven to constant weight, ground into uniform powder by an agate mortar, wrapped by tinfoil, and placed in a dryer for storage to determine the content and the ratio of the stable isotopes.
Further, the step (2) is specifically to measure and calculate TOC, TON (percentage content of stable isotope C \ N in organism, measured by instrument) and Delta in the sample by using a stable isotope mass spectrometer (Flash EA 1112 HT-Delta V Advantages, Thermo Co., Ltd.)13CTOCAnd delta15NTONA value; wherein the flow rate of the carrier gas He is 90mL/min, the temperature of the reaction tube is 960 ℃, and the temperature of the chromatographic column is 50 ℃.
Further, δ13CTOCThe values are based on the international standard (VPDB), delta13CTOCThe values are calculated according to the following formula:
wherein R is: (13C/12C)VPDBCarbon isotopic abundance ratio of VPDB, R: (A), (B), (C) and C) a13C/12C)sampleIs the ratio of two carbon isotopes in the sample (obtained by on-line detection with a stable isotope mass spectrometer), delta13CTOCThe analysis precision of the value is +/-0.2 per mill;
δ15NTONvalues are based on air nitrogen as reference, delta15NTONThe values are calculated according to the following formula:
wherein R is: (15N/14N)airIs the nitrogen isotope abundance ratio of nitrogen in air, R: (15N/14N)sampleIs the ratio of two nitrogen isotopes in the sample (from a stable isotope mass spectrometer)Obtained by online detection), delta15NTONThe analytical accuracy of the value was ± 0.25%.
Further, in step (3), the values are respectively delta13CTOCAnd delta15NTONDrawing a two-dimensional point set of carbon and nitrogen stable isotope ratio values of each population with the values of horizontal and vertical coordinates, and calculating convex deformation total areas TA (total area), standard Ellipse areas SEA (standard Ellipse areas) and nutrition ecological niche overlapping areas OA (overlap area) surrounded by dotted line connecting lines of outer edge points of the two-dimensional point set; calculating the total area TA, the standard elliptical area SEA and the overlap area OA respectively through R software; the values of the parameters obtained are as follows:
further, the step (4) is that according to the data analysis result in the step (3), the population with serious interspecific competition is subjected to targeted fishing and fishing, and the number of the related species population is supplemented to the white space niche. Therefore, the inter-species competition strength can be reduced, reasonable and efficient utilization of sea area environment resources and bait resources is researched, and finally natural sustainable development of the environment, the resources and the organisms in the marine ecosystem is realized.
The invention has the beneficial effects that: the invention introduces the interaction relation between the structure and the process of the ecological system under the bidirectional action of multiple pressure stresses and repair measures with multiple local scales, and the synergistic or antagonistic counteraction effect exists between the pressure stresses of different types and the treatment effect of the multi-nutrition-level biological resource maintenance technology, thereby obtaining the arrangement mode of artificial breeding facilities with scientific basis and the quantity of fishes suitable for releasing, and realizing the scientific reconstruction and the synergistic repair of the food net of the marine ranching.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a technical flow diagram of the present invention;
FIG. 2 is a schematic diagram of the vegetative niches of 3 species of fish in a certain marine ranch.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
taking the sea area around the temple island in the cigarette platform city of Shandong province as an experimental area, performing fishery resource investigation on the ecological breeding area of the temple island in half bay in 2018 for 11 months, and performing nutritional ecological niche evaluation on major species Sebastes schlegeli, hexagrammos otakii and sebastes marmoratus by using a stable isotope technology, wherein the method comprises the following steps of:
(1) sample collection and processing
Samples of swim life were taken from the sea in the area under investigation according to GB/T12763.1-2007 Marine survey Specification. The sample composition mainly comprises fish, shrimp, crab and sea cucumber, wherein the body muscle of the fish, shrimp and sea cucumber is taken, and the large turtle muscle of the crab is taken. The sample is dried for 48h at 60 ℃ in a drying oven to constant weight, ground into uniform powder by an agate mortar, wrapped by tinfoil, and placed in a dryer for storage to determine the content and the ratio of the stable isotopes.
(2) Determination of stable isotopes
Determination of TOC, TON and calculation of Delta in samples by in-line stable isotope mass spectrometry (Flash EA 1112 HT-Delta V Advantages, Thermo Co.)13CTOCAnd delta15NTONThe value is obtained. The flow rate of the carrier gas He is 90mL/min, the temperature of the reaction tube is 960 ℃, and the temperature of the chromatographic column is 50 ℃.
δ13CTOCThe values are based on the international standard (VPDB), delta13CTOCThe values are calculated according to the following formula:
wherein R is: (13C/12C)VPDBCarbon isotopic abundance ratio of VPDB, R: (A), (B), (C) and C) a13C/12C)sampleIs the ratio of two carbon isotopes in the sample, delta13CTOCThe analytical accuracy of the value was ± 0.2%.
δ15NTONValues are based on air nitrogen as reference, delta15NTONThe values are calculated according to the following formula:
wherein R is: (15N/14N)airIs the nitrogen isotope abundance ratio of nitrogen in air, R: (15N/14N)sampleIs the ratio of two nitrogen isotopes in the sample, delta15NTONThe analytical accuracy of the value was ± 0.25%.
The detection values for each sample are as follows:
(3) estimation of nutrient niche
Are respectively expressed by delta13C、δ15And N is a horizontal coordinate and a vertical coordinate, drawing a two-dimensional point set of the carbon-nitrogen stable isotope ratio of each population, and calculating the total convex deformation area TA (total area), the standard elliptical area SEA (standard Ellipse areas) and the vegetative niche overlapping area OA (overlap area) which are surrounded by the dotted line connecting lines of the outer edge points of the two-dimensional point set. The total area TA, the standard elliptical area SEA and the overlap area OA are calculated by the R software, respectively. The following data were obtained with the accompanying figure 2:
| sebastiscus schlegeli (XSPY) | Hexagrammos otakii (DLLX) | Sebastiscus marmoratus (HCY) | |
| Total area of nutrient ecological niche (TA) | 2.584 | 1.647 | 2.636 |
| Standard oval area (SEA) | 0.816 | 0.580 | 0.788 |
And (4) conclusion: the A, B, C population has the overlapping of nutrition ecological niches, namely interspecific competition;
secondly, in A, B, C, the nutrition level of a population A is the highest, the nutrition level of a population C is the next time, and the nutrition level of a population B is the lowest;
③ the food types of the population B, C are not similar, and the food types of the population A, C are similar;
the food competition pressure of the population A, C is the greatest, the food competition pressure of the population B, C is the second best, and the food competition pressure of the population A, B is the smallest.
(4) Evaluating the stability of the food net, predicting the development direction of the food net and carrying out manual intervention.
According to the data analysis result of 4.3, the population with serious interspecific competition is subjected to targeted fishing and angling, the number of related species populations is supplemented to the empty ecological niche, the interspecific competition strength is reduced, reasonable and efficient utilization of sea area environment resources and bait resources is researched, and finally natural sustainable development of the environment, the resources and the organisms in the marine ecosystem is realized.
Claims (7)
1. A food net stability evaluation method based on stable isotope technology is characterized in that: by measuring and calculating delta in the sample13CTOCAnd delta15NTONValues, then respectively, of delta for the natural content of stable isotope in vivo in the species in the biological community of the investigation region13CTOCAnd delta15NTONThe value is horizontal and vertical coordinates, and a two-dimensional point set of the carbon and nitrogen stable isotope ratio of each population is drawn to obtain delta13Variation of C value (CR) and delta15And (3) five parameters of N value variation (NR), nutrition ecological niche area (TA), Standard Elliptical Area (SEA) and nutrition ecological niche Overlapping Area (OA) are adopted to finish the evaluation of the stability of the biological community food net in the research area.
2. The stable isotope technology-based food net stability evaluation method according to claim 1, wherein: the method comprises the following steps:
(1) collecting and processing the main consumer samples in the area;
(2) measuring the stable isotope;
(3) estimating the nutrition ecological niche;
(4) evaluating the stability of the food net, predicting the development direction of the food net and carrying out manual intervention.
3. The stable isotope technology-based food net stability evaluation method according to claim 2, wherein: specifically, the step (1) is to collect samples of swimming organisms from the sea in a research area according to GB/T12763.1-2007 ocean survey regulations; the sample composition mainly comprises fish, shrimp, crab and sea cucumber, wherein the body muscle of the fish, shrimp and sea cucumber is taken, and the large turtle muscle of the crab is taken; the sample is dried for 48h at 60 ℃ in a drying oven to constant weight, ground into uniform powder by an agate mortar, wrapped by tinfoil, and placed in a dryer for storage to determine the content and the ratio of the stable isotopes.
4. The stable isotope technology-based food net stability evaluation method according to claim 2, wherein: the step (2) is specifically to measure and calculate TOC, TON and Delta in the sample by using a stable isotope mass spectrometer (Flash EA 1112 HT-Delta V Advantages, Thermo company)13CTOCAnd delta15NTONA value; wherein the flow rate of the carrier gas He is 90mL/min, the temperature of the reaction tube is 960 ℃, and the temperature of the chromatographic column is 50 ℃.
5. The stable isotope technology-based food net stability evaluation method according to claim 4, wherein: delta13CTOCThe values are based on the international standard (VPDB), delta13CTOCThe values are calculated according to the following formula:
wherein R is: (13C/12C)VPDBCarbon isotopic abundance ratio of VPDB, R: (A), (B), (C) and C) a13C/12C)sampleIs the ratio of two carbon isotopes in the sample, delta13CTOCThe analysis precision of the value is +/-0.2 per mill;
δ15NTONvalues are based on air nitrogen as reference, delta15NTONThe values are calculated according to the following formula:
wherein R is: (15N/14N)airIs the nitrogen isotope abundance ratio of nitrogen in air, R: (15N/14N)sampleIs the ratio of two nitrogen isotopes in the sample, delta15NTONThe analytical accuracy of the value was ± 0.25%.
6. The stable isotope technology-based food net stability evaluation method according to claim 2, wherein: step (3) is to use delta respectively13C、δ15The N value is horizontal and vertical coordinates, a two-dimensional point set of the carbon and nitrogen stable isotope ratio of each population is drawn, and a convex deformation total area TA (total area), a standard Ellipse area SEA (standard Ellipse areas) and a nutrition ecological niche overlapping area OA (overlap area) which are surrounded by dotted line connecting lines of outer edge points of the two-dimensional point set are calculated; calculating the total area TA, the standard elliptical area SEA and the overlap area OA respectively through R software; the values of the parameters obtained are as follows:
7. the stable isotope technology-based food net stability evaluation method according to claim 2, wherein: and (4) performing targeted fishing and fishing on the population with serious interspecific competition according to the data analysis result in the step (3), and supplementing the population quantity of related species to the white space niche.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110749417.8A CN113358734A (en) | 2021-07-02 | 2021-07-02 | Food net stability evaluation method based on stable isotope technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110749417.8A CN113358734A (en) | 2021-07-02 | 2021-07-02 | Food net stability evaluation method based on stable isotope technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113358734A true CN113358734A (en) | 2021-09-07 |
Family
ID=77537936
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110749417.8A Pending CN113358734A (en) | 2021-07-02 | 2021-07-02 | Food net stability evaluation method based on stable isotope technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113358734A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114137058A (en) * | 2021-11-04 | 2022-03-04 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Method for investigating food competition relationship among large jellyfish species and application thereof |
| CN114594184A (en) * | 2022-03-10 | 2022-06-07 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artificial release effect evaluation method based on stable isotope technology |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107169679A (en) * | 2017-06-22 | 2017-09-15 | 北京师范大学 | A kind of method of utilization food web estimation of stability Wetland ecological moisturizing implementation result |
| CN112862255A (en) * | 2021-01-13 | 2021-05-28 | 交通运输部天津水运工程科学研究所 | Fishway effect evaluation method based on stable isotope method |
-
2021
- 2021-07-02 CN CN202110749417.8A patent/CN113358734A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107169679A (en) * | 2017-06-22 | 2017-09-15 | 北京师范大学 | A kind of method of utilization food web estimation of stability Wetland ecological moisturizing implementation result |
| CN112862255A (en) * | 2021-01-13 | 2021-05-28 | 交通运输部天津水运工程科学研究所 | Fishway effect evaluation method based on stable isotope method |
Non-Patent Citations (1)
| Title |
|---|
| 盖珊珊等: "小黑山岛人工鱼礁区许氏平鲉和大泷六线鱼的营养生态位研究", 《生态学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114137058A (en) * | 2021-11-04 | 2022-03-04 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Method for investigating food competition relationship among large jellyfish species and application thereof |
| CN114594184A (en) * | 2022-03-10 | 2022-06-07 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artificial release effect evaluation method based on stable isotope technology |
| CN114594184B (en) * | 2022-03-10 | 2023-09-19 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Artificial releasing effect evaluation method based on stable isotope technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113358734A (en) | Food net stability evaluation method based on stable isotope technology | |
| Hessen et al. | Phytoplankton contribution to sestonic mass and elemental ratios in lakes: implications for zooplankton nutrition | |
| CN103353508B (en) | Method for predicting dry matter digestion rate and metabolic energy of grass | |
| CN110444295A (en) | A kind of marine product heavy metal health risk assessment method based on Monte Carlo method | |
| CN112862255B (en) | Fishway effect evaluation method based on stable isotope method | |
| Zhu et al. | Characterizing CH4 and N2O emissions from an intensive dairy operation in summer and fall in China | |
| CN107169679B (en) | Method for evaluating wetland ecological water replenishing implementation effect by using food net stability | |
| CN106408114A (en) | Application of multivariate linear regression model to predicting chicken abdominal fat quantity or breeding low fat chicken | |
| CN110440969B (en) | Quick patinopecten yessoensis vitality evaluation method | |
| Wang et al. | Effects of polyunsaturated fatty acids supplementation on the meat quality of pigs: a meta-analysis | |
| Bayne et al. | Seasonal variability in feeding behaviour, metabolic rates and carbon and nitrogen balances in the Sydney oyster, Saccostrea glomerata (Gould) | |
| CN102217739A (en) | Low-nitrogen-emission daily ration for growing pigs | |
| CN107094689B (en) | A method of identifying the fresh and alive Bostrichthys sinensis of separate sources | |
| CN103472194A (en) | Method for measuring and calculating threshold concentration of ecological toxic effect of environmental pollutants | |
| CN103583468B (en) | Method for rapidly evaluating corn DDGS fowl metabolic energy through physicochemical indexes | |
| CN105137022A (en) | Zebrafish detoxification and sensitivity evaluation method for conducting on-line water quality monitoring | |
| CN110794024A (en) | Standardized determination method for shellfish blood physiological indexes | |
| CN104006767B (en) | High-throughput plant type measurement method based on image processing | |
| CN110766282A (en) | Wetland purification capacity assessment and improvement method | |
| CN103499676A (en) | Method for estimating excretion amount of nitrogen, phosphorus and organic carbon in marine shellfish aquaculture | |
| CN113111294A (en) | Water body nutrition evaluation method based on algae diversity index and application thereof | |
| CN114594184B (en) | Artificial releasing effect evaluation method based on stable isotope technology | |
| Kjeld et al. | Allometry (scaling) of blood components in mammals: connection with economy of energy? | |
| Tuo et al. | Discrimination of sexual dimorphism through external morphology of spotted steed (Hemibarbus maculatus) in the Yuanhe River, China. | |
| CN110361291A (en) | A method of utilizing moisture content rapid evaluation pork intramuscular fat content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210907 |
|
| RJ01 | Rejection of invention patent application after publication |