CN113350506A - Preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer - Google Patents

Preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer Download PDF

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CN113350506A
CN113350506A CN202110716018.1A CN202110716018A CN113350506A CN 113350506 A CN113350506 A CN 113350506A CN 202110716018 A CN202110716018 A CN 202110716018A CN 113350506 A CN113350506 A CN 113350506A
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silk fibroin
solution
photosensitizer
nanoparticles
regenerated silk
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许志刚
许姬明
高永娥
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Southwest University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A61K41/0057—Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
    • A61K41/0071—PDT with porphyrins having exactly 20 ring atoms, i.e. based on the non-expanded tetrapyrrolic ring system, e.g. bacteriochlorin, chlorin-e6, or phthalocyanines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/513—Organic macromolecular compounds; Dendrimers
    • A61K9/5169—Proteins, e.g. albumin, gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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Abstract

The invention particularly designs preparation of nanoparticles based on regenerated silk fibroin combined with a photosensitizer and antitumor research thereof. The synthesis steps are as follows: (1) degumming silkworm cocoons to extract silk fibroin and preparing a regenerated silk fibroin RSF solution; (2) and physically embedding the photosensitizer Ce6 in silk fibroin by adopting a solvent removal method to prepare the silk fibroin nanoparticle SFC NPs loaded with Ce 6. The prepared nano particles have uniform size and good stability in aqueous solution, and have basically negligible toxic and side effects and good treatment effect in tumor treatment. Meanwhile, near-infrared fluorescence of the photosensitizer Ce6 is used for imaging, and diagnosis and treatment integration is realized.

Description

Preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer
Technical Field
The invention relates to the field of chemical drugs and biological application, in particular to a preparation method of nanoparticles based on regenerated silk fibroin combined with a photosensitizer Ce6 and application of the nanoparticles in anti-tumor drug delivery.
Background
Chlorins e6 (Chlorin e6, Ce 6) are typically synthesized from pheophorbide a, with CAS number: 19660-77-6, chemical structural formula: c34H36N4O6Relative molecular weight: 596.67. the singlet oxygen generated has high efficiency, so the singlet oxygen is a good photosensitizer for tumor photodynamic therapy. But because of itHydrophobicity, there is a certain obstacle in practical operation.
The photodynamic therapy is that the photosensitizer is used to transfer energy to surrounding oxygen after being excited under the irradiation of specific wavelength, and a large amount of ROS is generated to generate cytotoxicity, so that the effect of treatment is achieved by killing tumor cells. Photodynamic therapy is a novel therapy for cancer therapy, and three conditions required for its action are: oxygen, photosensitizer, and visible light. The photosensitizer chlorin e6 has the problems of strong hydrophobicity, low size, easy aggregation in solution and the like, like most photosensitizers, so that the hydrophobic photosensitizer chlorin e6 is delivered to tumors through a drug carrier, the loss of chlorin e6 in blood circulation is effectively reduced, the damage to normal tissues is reduced, the drug activity can be maintained, the tumors are killed, and a good treatment effect is obtained. The hydrophobic photosensitizer chlorin e6 is directly wrapped in the regenerated silk fibroin by a solvent method through a physical embedding method, the preparation method is simple and direct, and the method is expected to become a method for treating clinical tumors in the future.
The silk fibroin is an important component of silk, is a natural protein polymer macromolecule and accounts for 70-80% of the silk. Has excellent mechanical property, water permeability, oxygen permeability, biocompatibility and non-inflammatory characteristic, and is beneficial to cell adhesion. Therefore, the nano-drug system based on the combination of the regenerated silk fibroin and the photosensitizer can be self-assembled to form nanospheres, the drug loading amount is high, the toxic and side effects are low, the problem of hydrophobic drugs in the process of cancer photodynamic therapy can be effectively solved, the aggregation is avoided due to the good stability of the nano-drug system, and the nano-drug system can be accumulated at tumor positions. Therefore, the design and construction of a nano-drug delivery system with low toxic and side effects and high biological safety function are strongly necessary.
Disclosure of Invention
Aiming at the problems of poor stability, poor biological safety and the like of micelles in the current drug delivery system, the invention provides a preparation method of nanoparticles of a regenerated silk fibroin-combined photosensitizer, wherein a photosensitizer chlorin e6 is directly physically embedded in regenerated silk fibroin by a solvent method. And the treatment effect is discussed through experiments such as cytotoxicity, in vivo photodynamic and the like. The nano-particles synthesized by the method have good biocompatibility and dispersibility, and generate a large amount of ROS to kill tumor cells under certain illumination conditions, so that effective delivery and in-vivo treatment of the medicine are realized.
The technical scheme of the invention is as follows:
a preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer is characterized by comprising the following steps:
(1) the method for preparing the regenerated silk fibroin RSF solution by extracting silk fibroin based on silkworm cocoon degumming comprises the following steps: weighing 10g of silkworm cocoon, placing the silkworm cocoon in 1000ml of sodium carbonate solution, boiling for degumming for 30 minutes, slowly stirring every 10 minutes, repeatedly washing for 2-3 times by using secondary water for purification, and placing the degummed silk fibroin in an oven for overnight at 40 ℃; at 90 ℃ in CaCl2Adding appropriate amount of degummed silk fibroin into the absolute ethyl alcohol/secondary water solution, and stirring for 2-2.5 hours to fully dissolve the silk fibroin; filtering with 8 layers of gauze, putting into a dialysis bag with cut-off molecular weight Da =14000, and dialyzing for about 3 days; centrifuging at 9000rpm for 10 min, sucking the upper layer solution, and concentrating the obtained silk fibroin solution in polyethylene glycol solution with molecular weight of 20000 for 24 hr to obtain regenerated silk fibroin RSF solution;
(2) physically embedding a photosensitizer Ce6 in silk fibroin by adopting a solvent removal method to prepare the silk fibroin nanoparticle SFC NPs loaded with Ce6, comprising the following steps: slowly adding 1ml of regenerated silk fibroin solution dropwise into 9ml of acetone solution dissolved with Ce6, stirring vigorously for 5-6 minutes, performing ultrasonic treatment with a cell disruption instrument for 30 seconds, stopping ultrasonic treatment for 2 seconds every 2 seconds, and stirring at normal temperature overnight; subsequently, the solution was centrifuged at 12000rpm for about 15 minutes in a centrifuge, and the precipitate was collected and washed several times with secondary water, and finally dispersed in the secondary water to obtain a solution of Ce 6-loaded silk fibroin nanoparticles SFC NPs.
Further, the mass ratio of sodium carbonate in the sodium carbonate solution in the step (1) is 0.2%; CaCl in the step (1)2Anhydrous ethanol/CaCl in secondary aqueous solution2The molar ratio of the absolute ethyl alcohol to the secondary water is 1: 2: 8; the mass of the polyethylene glycol in the polyethylene glycol solution in the step (1) accounts for 8 percent; the diameter range of the RSF nano-particles in the regenerated silk fibroin RSF solution in the step (1) is 100-140 nm.
Further, the concentration of the photosensitizer Ce6 in the acetone solution in the step (2) is 140 mu g ml-1-160 μg ml-1(ii) a The concentration of the regenerated silk fibroin solution in the step (2) is 13 mg mL-1-16 mg mL-1(ii) a The diameter range of the SFC NPs nanoparticles in the step (2) is 130-170 nm.
The invention has the following main advantages:
1. the method aims at solving the problems existing in the current drug delivery system, such as poor stability and high toxicity of the nanoparticles. The project creatively provides a drug delivery system based on regenerated silk fibroin combined with a photosensitizer, and the nano-particles utilize the good biocompatibility and stability of the regenerated silk fibroin to regulate and control the size and stability of the nano-particles, so that partial problems in the current drug delivery system are solved, and the drug delivery system has certain guiding significance for effectively treating tumors.
2. The delivery carrier and the drug molecules are designed and synthesized to physically embed the photosensitizer to form the nano particles by a solvent method, the method is simple and easy to implement, and the obtained nano particles have good stability and dispersibility. Due to the existence of the photosensitizer, ROS is generated under the irradiation of laser to kill tumor cells, thereby realizing the treatment of cancer.
Drawings
In order to make the purpose, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings:
fig. 1 is a schematic flow chart of the preparation of nanoparticles of regenerated silk fibroin-conjugated photosensitizer in example 1.
Fig. 2 is a TEM and DLS schematic of the regenerated silk fibroin photosensitizer-bound nanoparticles of example 1.
Fig. 3 is a schematic diagram of uv absorption and fluorescence of the regenerated silk fibroin photosensitizer-bound nanoparticles of example 1.
Fig. 4 is a schematic representation of the cytotoxicity of the regenerated silk fibroin photosensitizer-bound nanoparticles of example 1.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 regenerated silk fibroin photosensitizer-bound nanoparticles
The following preparation was carried out according to the schematic flow diagram of regenerated silk fibroin photosensitizer-bound nanoparticles shown in fig. 1:
(1) the method for preparing the regenerated silk fibroin RSF solution by extracting silk fibroin based on silkworm cocoon degumming comprises the following steps: weighing 10g of silkworm cocoon, placing the silkworm cocoon in 1000ml of sodium carbonate solution with the mass fraction of 0.2%, boiling for degumming for 30 minutes, slowly stirring every 10 minutes, repeatedly washing for 2-3 times by using secondary water for purification, and placing the degummed silk fibroin in an oven for overnight at 40 ℃; at 90 ℃, in a molar ratio of 1: 2: 8 CaCl2Adding appropriate amount of degummed silk fibroin into the absolute ethyl alcohol/secondary water solution, and stirring for 2-2.5 hours to fully dissolve the silk fibroin; filtering with 8 layers of gauze, putting into a dialysis bag with cut-off molecular weight Da =14000, and dialyzing for about 3 days; centrifuging at 9000rpm for 10 min, sucking the upper layer solution after centrifugation, and concentrating the obtained silk fibroin solution in polyethylene glycol solution with molecular weight of 20000 mass percent of 8% for 24 hr to obtain regenerated silk fibroin RSF solution; the RSF nanoparticles have a diameter range of about 120 nm.
(2) Physically embedding a photosensitizer Ce6 in silk fibroin by adopting a solvent removal method to prepare the silk fibroin nanoparticle SFC NPs loaded with Ce6, comprising the following steps: the concentration of dissolved Ce6 is 150 mug ml-1To 9mL of acetone solution, 1mL of a solution having a concentration of 13 mg mL was slowly added dropwise-1Regenerating the silk fibroin solution, stirring vigorously for 5-6 minutes, performing ultrasonic treatment for 30 seconds by using a cell disruption instrument, stopping ultrasonic treatment for 2 seconds every 2 seconds, and stirring at normal temperature overnight after finishing ultrasonic treatment; subsequently, the solution was centrifuged at 12000rpm for about 15 minutes in a centrifuge, and the precipitate was collected and washed several times with secondary water, and finally dispersed in the secondary water to obtain a solution of Ce 6-loaded silk fibroin nanoparticles SFC NPs. Alignment of SFC NPs nanoparticlesThe diameter range is about 150 nm.
TEM and DLS schematic of regenerated silk fibroin photosensitizer-binding nanoparticles
The morphological characteristics of the SF NPs were observed using a transmission electron microscope and their hydrated particle size was measured using a Zeta potentiometer. As shown in FIG. 2, the diameter range of SF NPs nanoparticles is around 150 nm.
Ultraviolet absorption and fluorescence schematic of regenerated silk fibroin photosensitizer-bound nanoparticles
Ultraviolet absorption and fluorescence emission were detected using an ultraviolet spectrophotometer and a fluorescence spectrophotometer, respectively. As shown in FIG. 3, from the ultraviolet absorption spectrum, the photosensitizer Ce6 has absorption peaks at 400nm and 660nm, and although the SF NPs nanoparticles have red shift at 660nm, the SF NPs nanoparticles also have a strong absorption peak at 400 nm; in addition, from the fluorescence emission spectrum, the SF NPs nanoparticles and the photosensitizer Ce6 have stronger emission peaks near 660nm, and both can indicate that the SF NPs nanoparticles are successfully prepared.
Cytotoxicity assays of nanoparticles of regenerated silk fibroin-conjugated photosensitizers
The in vitro cytotoxicity of the regenerated silk fibroin combined with Ce6 nanoparticle SFC NPs on mouse breast cancer cells 4T1 was determined by MTT method. At a rate of 1X 10 per hole4Cell density cells were incubated overnight in 96-well plates, after cells were attached to the wall, the photosensitizers chlorin e6 and SFC NPs were added to the plates at different concentrations, respectively, and incubation was continued for 4-6 hours. The light groups were irradiated with a near infrared laser at 660nm for 5 minutes. Old medium was removed and incubation continued for 24 hours with new medium. After the incubation was completed, the cells were gently washed with PBS, and 100 μ L of fresh medium containing 10% MTT reagent was added to each well and incubated for 4 hours. Finally, 150 μ of LDMSO was added to the well plate, and the absorbance was measured using a microplate reader. The cell theoretical survival rate was 100% using the blank control group as a control. The control groups were compared and the cell viability was calculated for the remaining groups and plotted. As shown in FIG. 4, the difference in cytotoxicity of free Ce6 and SFC NPs on cells was smaller for the non-illuminated group (left) and stronger for the illuminated group (right) than for the SFC NPsLeaving Ce 6. When the concentration of the drug is 3 mug/mL, the inhibition rate of the SFC NPs of the illumination group to 4T1 cells is ninety percent, and the inhibition rate of the free Ce6 of the illumination group to 4T1 cells is eighty percent, which proves that the SFC NPs of the regenerated silk fibroin combined with the photosensitizer have obvious treatment effect.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims (3)

1. A preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer is characterized by comprising the following steps:
(1) the method for preparing the regenerated silk fibroin RSF solution by extracting silk fibroin based on silkworm cocoon degumming comprises the following steps: weighing 10g of silkworm cocoon, placing the silkworm cocoon in 1000ml of sodium carbonate solution, boiling for degumming for 30 minutes, slowly stirring every 10 minutes, repeatedly washing for 2-3 times by using secondary water for purification, and placing the degummed silk fibroin in an oven for overnight at 40 ℃; at 90 ℃ in CaCl2Adding appropriate amount of degummed silk fibroin into the absolute ethyl alcohol/secondary water solution, and stirring for 2-2.5 hours to fully dissolve the silk fibroin; filtering with 8 layers of gauze, putting into a dialysis bag with cut-off molecular weight Da =14000, and dialyzing for about 3 days; centrifuging at 9000rpm for 10 min, sucking the upper layer solution, and concentrating the obtained silk fibroin solution in polyethylene glycol solution with molecular weight of 20000 for 24 hr to obtain regenerated silk fibroin RSF solution;
(2) physically embedding a photosensitizer Ce6 in silk fibroin by adopting a solvent removal method to prepare the silk fibroin nanoparticle SFC NPs loaded with Ce6, comprising the following steps: slowly adding 1ml of regenerated silk fibroin solution dropwise into 9ml of acetone solution dissolved with Ce6, stirring vigorously for 5-6 minutes, performing ultrasonic treatment with a cell disruption instrument for 30 seconds, stopping ultrasonic treatment for 2 seconds every 2 seconds, and stirring at normal temperature overnight; subsequently, the solution was centrifuged at 12000rpm for about 15 minutes in a centrifuge, and the precipitate was collected and washed several times with secondary water, and finally dispersed in the secondary water to obtain a solution of Ce 6-loaded silk fibroin nanoparticles SFC NPs.
2. The method for preparing nanoparticles by using regenerated silk fibroin combined with photosensitizer as claimed in claim 1, characterized in that: the mass ratio of sodium carbonate in the sodium carbonate solution in the step (1) is 0.2%; CaCl in the step (1)2Anhydrous ethanol/CaCl in secondary aqueous solution2The molar ratio of the absolute ethyl alcohol to the secondary water is 1: 2: 8; the mass of the polyethylene glycol in the polyethylene glycol solution in the step (1) accounts for 8 percent; the diameter range of the RSF nano-particles in the regenerated silk fibroin RSF solution in the step (1) is 100-140 nm.
3. The method for preparing nanoparticles by using regenerated silk fibroin combined with photosensitizer as claimed in claim 1, characterized in that: the concentration of the photosensitizer Ce6 in the acetone solution in the step (2) is 140 mu g ml-1-160 μg ml-1(ii) a The concentration of the regenerated silk fibroin solution in the step (2) is 13 mg mL-1-16 mg mL-1(ii) a The diameter range of the SFC NPs nanoparticles in the step (2) is 130-170 nm.
CN202110716018.1A 2021-06-28 2021-06-28 Preparation method of nanoparticles of regenerated silk fibroin combined photosensitizer Pending CN113350506A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116920110A (en) * 2023-07-28 2023-10-24 深圳市儿童医院 Multifunctional nano platform based on aggregation-induced emission and gene editing, and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110251480A (en) * 2019-06-26 2019-09-20 浙江大学 A kind of fibroin albumen of core-shell structure/manganese dioxide complex microsphere pharmaceutical carrier and preparation method
CN110354272A (en) * 2019-07-10 2019-10-22 浙江理工大学 A kind of preparation method for tumor post-operation radiotherapy photo-thermal-light power link treatment controlled release hydrogel

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110251480A (en) * 2019-06-26 2019-09-20 浙江大学 A kind of fibroin albumen of core-shell structure/manganese dioxide complex microsphere pharmaceutical carrier and preparation method
CN110354272A (en) * 2019-07-10 2019-10-22 浙江理工大学 A kind of preparation method for tumor post-operation radiotherapy photo-thermal-light power link treatment controlled release hydrogel

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEI ZHANG等: ""MnO2-capped silk fibroin (SF) nanoparticles with chlorin e6 (Ce6) encapsulation for augmented photo-driven therapy by modulating the tumor microenvironment"", 《J. MATER. CHEM. B》, vol. 9, 5 May 2021 (2021-05-05), pages 3678 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116920110A (en) * 2023-07-28 2023-10-24 深圳市儿童医院 Multifunctional nano platform based on aggregation-induced emission and gene editing, and preparation method and application thereof
CN116920110B (en) * 2023-07-28 2024-05-14 深圳市儿童医院 Multifunctional nano platform based on aggregation-induced emission and gene editing, and preparation method and application thereof

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Application publication date: 20210907