CN113350229A - Glutathione-rich yeast extract, and preparation method and application thereof - Google Patents
Glutathione-rich yeast extract, and preparation method and application thereof Download PDFInfo
- Publication number
- CN113350229A CN113350229A CN202110664656.3A CN202110664656A CN113350229A CN 113350229 A CN113350229 A CN 113350229A CN 202110664656 A CN202110664656 A CN 202110664656A CN 113350229 A CN113350229 A CN 113350229A
- Authority
- CN
- China
- Prior art keywords
- glutathione
- yeast extract
- preparation
- yeast
- percent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a glutathione-enriched yeast extract, a preparation method and application thereof. The content of the reduced glutathione is more than 5 percent, the content of the protein is more than 35 percent, the DPPH free radical clearance rate is 15-20 percent, the half inhibition concentration IC50 of the tyrosinase is less than or equal to 0.3mg/L, the intermediate antioxidant activity is realized, the dark state of the pigment and the formation of free radicals caused by the overexpression of the tyrosinase in the organism can be inhibited, and the skin color is lightened to achieve the whitening antioxidant effect.
Description
Technical Field
The invention relates to the field of yeast extracts, in particular to a glutathione-rich yeast extract, a preparation method and application thereof.
Background
The yeast extract is rich in functional components such as glutathione, other oligopeptides, amino acids and the like, and is widely applied to industries such as food seasoning, beverages and the like, wherein the glutathione containing sulfhydryl cysteine residues is a main intracellular antioxidant in organisms.
At present, the production process of yeast extract can be roughly divided into: autolysis, enzymolysis, solid-liquid separation, high-temperature concentration of clear liquid and other processes to obtain an extract product which has special flavor and color and contains a certain amount of peptide and amino acid. The yeast extract prepared by the prior art can promote the generation of Maillard reaction in the production process so as to improve the flavor of products, and is mainly applied to the field of seasonings.
Chinese patent CN105919137 discloses a high-protein yeast extract and a preparation method thereof. According to the invention, yeast milk is obtained by yeast fermentation, autolysis enzymolysis is carried out on the yeast milk, auxiliary RNA is added in the treatment process to obtain the yeast extract with the protein content of more than 59%, and finally the yeast extract with the I + G content of more than 18% is obtained by ultrafiltration concentration, so that the requirements of people are met, and the economic value is improved.
Chinese patent CN101756151 discloses a homoglutamic acid yeast extract and a preparation method thereof, the homoglutamic acid yeast extract is obtained by yeast fermentation, then autolysis enzymolysis is carried out on the yeast milk, enzyme is deactivated and drying is carried out, and the yeast extract is rich in glutamic acid, the content of the glutamic acid is more than 10 percent, the homoglutamic acid yeast extract has more delicious taste and stronger capability of strengthening the delicate flavor of food.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: the yeast extract produced by the prior art has low glutathione content, low free radical scavenging capacity and tyrosinase activity inhibition strength, and poor whitening and antioxidant effects when being used for cosmetics.
Aiming at the defects in the prior art, one of the purposes of the invention is to provide a glutathione-rich yeast extract, the other purpose of the invention is to provide the use of the yeast extract or the yeast extract prepared by the preparation method for antioxidation, the fourth purpose of the invention is to provide the use of the yeast extract or the yeast extract prepared by the preparation method for whitening, the fifth purpose of the invention is to provide the use of the yeast extract or the yeast extract prepared by the preparation method in the cosmetic field, and the sixth purpose of the invention is to provide a whitening antioxidation facial mask containing the yeast extract or the yeast extract prepared by the preparation method.
The technical scheme of the invention is as follows:
the invention provides a glutathione-enriched yeast extract, which comprises reducing glutathione and protein, wherein the content of the reducing glutathione is more than or equal to 5 percent and the content of the protein is more than or equal to 35 percent based on the mass of dry matters of the yeast extract, the DPPH free radical clearance rate of the glutathione-enriched yeast extract is 15-20 percent, and the tyrosinase half inhibition concentration IC50 of the glutathione-enriched yeast extract is less than or equal to 0.3 mg/L.
Preferably, the content of the reduced glutathione is more than or equal to 10 percent, the content of the protein is more than or equal to 40 percent, and the tyrosinase half inhibition concentration IC50 of the glutathione-rich yeast extract is less than or equal to 0.2 mg/L.
The invention also provides a preparation method of the glutathione-enriched yeast extract, which comprises the following steps:
(1) homogenizing and breaking the walls of the glutathione-rich yeast raw material under high pressure to obtain yeast cell wall milk;
(2) performing enzymolysis on the yeast cell wall milk obtained in the step (1) by using a complex enzyme, wherein the complex enzyme is two or more of cellulase, glucanase and mannanase;
(3) and (3) sequentially carrying out enzyme deactivation treatment and centrifugal separation on the zymolyte obtained in the step (2), and taking supernatant fluid to obtain the glutathione-enriched yeast extract.
Preferably, before high-pressure homogenizing wall breaking, a saccharomyces cerevisiae strain is adopted, molasses is used as a carbon source, ammonium sulfate is used as a nitrogen source, fermentation is carried out for 20-22 hours at the temperature of 28-32 ℃ and at the pH value of 5.5-6.5, a glutathione-rich yeast raw material is obtained, preferably, the glutathione-rich yeast raw material can be in a yeast milk or dry yeast form, preferably, 6-15 wt% of yeast milk is prepared, and further preferably, the saccharomyces cerevisiae is a yeast strain (saccharomyces cerevisiae) rich in reduced glutathione, and the preservation number is as follows: CCTCC M205130, the preservation address is: the strain was deposited in the China center for type culture Collection (Wuhan) at 25.10.2005 and described in the patent publication No. CN 101575578A.
Preferably, the preparation method further comprises concentrating or drying the glutathione-enriched yeast extract to obtain a paste or powder product.
Preferably, in step (1), the high-pressure homogeneous wall breaking is performed at a pH of 1.0 to 7.0 and a temperature of 4 to 50 ℃, preferably at a pH of 3.0 to 5.0 and a temperature of 10 to 40 ℃, more preferably at a pressure of 5 to 100MPa, and preferably at a pressure of 10 to 80 MPa.
Preferably, in the step (2), the addition amount of the complex enzyme is 0.1-3% by mass of the dry substance of the yeast cell wall milk, and is preferably 1-2%.
Preferably, in the step (2), the addition amount of the cellulase is 0.5-1% and the addition amount of the glucanase is 0.5-1% by mass of the dry substance of the yeast cell wall milk; or the addition amount of the cellulase is 0.5-1%, and the addition amount of the mannosidase is 0.5-1%; or the addition amount of the glucanase is 0.5-1%, and the addition amount of the mannase is 0.5-1%.
Preferably, in step (2), the temperature of the enzymolysis is 10-50 ℃, and the pH is 1.0-8.0, preferably, the temperature of the enzymolysis is 25-45 ℃, and the pH is 2.0-6.0, further preferably, the enzymolysis time is 2-15h, preferably 3-12 h.
Preferably, in step (3), the enzyme deactivation is performed at a temperature of 50 to 80 ℃ for 0.5 to 1.5 hours.
The invention also provides the use of the yeast extract or the yeast extract prepared by the preparation method for antioxidation.
The invention also provides the application of the yeast extract or the yeast extract prepared by the preparation method in preparing the tyrosinase inhibitor.
The invention also provides application of the yeast extract or the yeast extract prepared by the preparation method in the field of cosmetics, preferably whitening antioxidant cosmetics.
The invention also provides a whitening antioxidant mask containing the yeast extract or the yeast extract prepared by the preparation method, which comprises the following components in percentage by weight, 5-10% of yeast extract rich in glutathione, 1-8% of propylene glycol, 8-15% of acrylamide dimethyl taurinate/VP copolymer (ice crystal forming agent), 1-3% of glycerol, 1-3% of carboxymethyl chitosan, 1-3% of hyaluronic acid, 0-1% of grapefruit essence and 1-5% of phenoxyethanol, and the balance of water.
The invention has the beneficial effects that:
the glutathione-rich yeast extract prepared by the invention has the reduced glutathione content of more than 5 percent, the protein content of more than 35 percent, the DPPH free radical clearance rate of 15-20 percent and the tyrosinase half inhibition concentration IC50 of less than or equal to 0.3mg/L, has medium antioxidant activity, can inhibit the dark state of pigment and the formation of free radicals caused by the overexpression of tyrosinase in organisms, thereby lightening the skin color and achieving the whitening and antioxidant effects.
Detailed Description
In order to better understand the technical solutions, the technical solutions of the present application are described in detail with specific embodiments below, and it should be understood that the specific features in the embodiments and examples of the present application are detailed descriptions of the technical solutions of the present application, but not limitations of the technical solutions of the present application, and the technical features in the embodiments and examples of the present application may be combined with each other without conflict.
The invention provides a glutathione-enriched yeast extract, which comprises reducing glutathione and protein, wherein the content of the reducing glutathione is more than or equal to 5 percent and the content of the protein is more than or equal to 35 percent based on the mass of dry matters of the yeast extract, the DPPH free radical clearance rate of the glutathione-enriched yeast extract is 15-20 percent, and the tyrosinase half inhibition concentration IC50 of the glutathione-enriched yeast extract is less than or equal to 0.3 mg/L.
Preferably, the content of the reduced glutathione is more than or equal to 10 percent, the content of the protein is more than or equal to 40 percent, and the tyrosinase half inhibition concentration IC50 of the glutathione-rich yeast extract is less than or equal to 0.2 mg/L.
According to the preparation method of the glutathione-enriched yeast extract, firstly, the reductive glutathione can be quickly discharged from cells through high-pressure homogeneous wall breaking, and the reductive glutathione is easily decomposed by endogenous enzymes, so that compared with autolytic wall breaking, the reductive glutathione can be kept as much as possible by adopting high-pressure homogeneous wall breaking; the inventor finds that the mannan and the beta-glucan can be degraded and the glutathione can be separated from the yeast cell wall by adopting two or more of cellulase, glucanase and mannanase. Because the glutathione is easily degraded by the protease, compared with the method adopting the protease for degradation, the composite enzyme can retain more reductive glutathione in the yeast, and improve the antioxidation and whitening effects of the yeast extract.
The invention also provides a preparation method of the glutathione-enriched yeast extract, which comprises the following steps:
(1) homogenizing and breaking the walls of the glutathione-rich yeast raw material under high pressure to obtain yeast cell wall milk;
(2) performing enzymolysis on the yeast cell wall milk obtained in the step (1) by using a complex enzyme, wherein the complex enzyme is two or more of cellulase, glucanase and mannanase;
(3) and (3) sequentially carrying out enzyme deactivation treatment and centrifugal separation on the zymolyte obtained in the step (2), and taking supernatant fluid to obtain the glutathione-enriched yeast extract.
Preferably, before high-pressure homogenizing wall breaking, a saccharomyces cerevisiae strain is adopted, molasses is used as a carbon source, ammonium sulfate is used as a nitrogen source, fermentation is carried out for 20-22 hours at the temperature of 28-32 ℃ and at the pH value of 5.5-6.5, a glutathione-rich yeast raw material is obtained, preferably, the glutathione-rich yeast raw material can be in a yeast milk or dry yeast form, preferably, 6-15 wt% of yeast milk is prepared, and further preferably, the saccharomyces cerevisiae is a yeast strain (saccharomyces cerevisiae) rich in reduced glutathione, and the preservation number is as follows: CCTCC M205130, the preservation address is: the strain was deposited in the China center for type culture Collection (Wuhan) at 25.10.2005 and described in the patent publication No. CN 101575578A.
Preferably, the preparation method further comprises concentrating or drying the glutathione-enriched yeast extract to obtain a paste or powder product
Preferably, in step (1), the high-pressure homogeneous wall breaking is performed at a pH of 1.0 to 7.0 and a temperature of 4 to 50 ℃, preferably at a pH of 3.0 to 5.0 and a temperature of 10 to 40 ℃, more preferably at a pressure of 5 to 100MPa, and preferably at a pressure of 10 to 80 MPa.
Preferably, in the step (2), the addition amount of the complex enzyme is 0.1-3% by mass of the dry substance of the yeast cell wall milk, and is preferably 1-2%.
Preferably, in the step (2), the addition amount of the cellulase is 0.5-1% and the addition amount of the glucanase is 0.5-1% by mass of the dry substance of the yeast cell wall milk; or the addition amount of the cellulase is 0.5-1%, and the addition amount of the mannosidase is 0.5-1%; or the addition amount of the glucanase is 0.5-1%, and the addition amount of the mannase is 0.5-1%.
Preferably, in step (2), the temperature of the enzymolysis is 10-50 ℃, and the pH is 1.0-8.0, preferably, the temperature of the enzymolysis is 25-45 ℃, and the pH is 2.0-6.0, further preferably, the enzymolysis time is 2-15h, preferably 3-12 h.
Preferably, in step (3), the enzyme deactivation is performed at a temperature of 50 to 80 ℃ for 0.5 to 1.5 hours.
The invention also provides the use of the yeast extract or the yeast extract prepared by the preparation method for antioxidation.
The invention also provides the application of the yeast extract or the yeast extract prepared by the preparation method in preparing the tyrosinase inhibitor.
The invention also provides application of the yeast extract or the yeast extract prepared by the preparation method in the field of cosmetics, preferably whitening antioxidant cosmetics.
The invention also provides a whitening antioxidant mask containing the yeast extract or the yeast extract prepared by the preparation method, which comprises the following components in percentage by weight, 5-10% of yeast extract rich in glutathione, 1-8% of propylene glycol, 8-15% of acrylamide dimethyl taurinate/VP copolymer (ice crystal forming agent), 1-3% of glycerol, 1-3% of carboxymethyl chitosan, 1-3% of hyaluronic acid, 0-1% of grapefruit essence and 1-5% of phenoxyethanol, and the balance of water.
DPPH is also called 1, 1-diphenyl-2-trinitrobenzene trap, is a very stable free radical with a nitrogen center, and the stability of DPPH comes from the steric hindrance of 3 benzene rings with resonance stabilization effect, so that unpaired electrons on the nitrogen atom in the middle cannot play the role of electron pairing. Its absolute ethyl alcohol solution is purple, and has maximum absorption at wavelength of 517nm, and its absorbance and concentration are in linear relation. When a radical scavenger is added thereto, DPPH may be combined with or substituted for the radical scavenger, so that the radical number is decreased and the absorbance becomes small, whereby the ability to clarify the radical can be evaluated.
The glutathione-enriched yeast extract and the preparation method thereof according to the present invention will be specifically described below by way of specific examples and experimental examples.
The inventive examples and comparative examples used starting materials and equipment sources as shown in table 1.
TABLE 1 examples of the invention and comparative examples use sources of raw materials and equipment
Example 1
The yeast preparation method comprises the following steps:
the yeast strain is a yeast strain (Saccharomyces cerevisiae) rich in reduced glutathione, the preservation number is CCTCC M205130, 1% of yeast extract, 2% of peptone and 2% of glucose are taken as nutrient solution, the yeast strain rich in reduced glutathione is inoculated, the common culture temperature is 30 ℃, the nutrient solution is added in a feeding and feeding manner in the fermentation process, and the first stage culture is carried out for 6 hours; the adding flow rate of the nutrient solution in the second stage is 20% of that in the first stage, and the culture is carried out for 8 h. And after the culture is finished, separating the fermentation liquor to obtain the yeast.
Preparing yeast into yeast milk with 10% (yeast dry matter content) in a mixing tank, adjusting pH to 3.0, heating to 30 deg.C, and homogenizing under 50 MPa. Adding the above solution into a reaction tank, adjusting pH to 6.0, maintaining the temperature at 40 deg.C, adding 1% cellulase and 1% dextranase, and performing enzymolysis for 3 hr. Then heating to 80 ℃, keeping the temperature for 0.5h to inactivate enzyme, and then centrifuging the enzymatic hydrolysate to obtain supernatant fluid, namely the glutathione-enriched yeast extract.
Example 2
Yeast preparation was as in example 1.
Preparing 15% (yeast dry matter content) yeast milk from the obtained yeast in a mixing tank, adjusting pH to 7.0, cooling to 4 deg.C, and homogenizing under 5 MPa. Adding the above solution into a reaction tank, adjusting pH to 1.0, heating to 10 deg.C, adding 0.05% cellulase and 0.05% mannosidase, and performing enzymolysis for 15 hr. Then heating to 70 ℃, keeping the temperature for 3h to inactivate enzyme, then centrifuging the enzymolysis liquid to obtain supernatant, and then concentrating the supernatant by adopting a multi-effect evaporation process until the mass concentration of dry matters is 60% to obtain a pasty glutathione-enriched yeast extract, wherein the evaporation temperature is 65 ℃.
Example 3
Yeast preparation was as in example 1.
Preparing 15% (yeast dry matter content) yeast milk from the above yeast in a mixing tank, adjusting pH to 2.0, controlling temperature at 10 deg.C, and homogenizing under 40 MPa. Adding the above solution into a reaction tank, adjusting pH to 2.0, controlling temperature at 30 deg.C, adding 0.5% cellulase and 1% dextranase, and performing enzymolysis for 12 hr. And then heating to 65 ℃, keeping the temperature for 1.5h to inactivate enzyme, centrifuging the enzymolysis liquid to obtain supernatant, and drying the supernatant in a spray dryer, wherein the inlet air temperature is 130 ℃, and the outlet air temperature is 80 ℃ to obtain the powdery yeast extract rich in glutathione.
Example 4
Yeast preparation was as in example 1.
Preparing the yeast into yeast milk with 10% (yeast dry matter content) in a mixing tank, adjusting pH to 6.0, controlling temperature at 20 deg.C, and homogenizing under 80 MPa. Adding the above solution into a reaction tank, adjusting pH to 4.0, controlling temperature at 25 deg.C, adding 1% dextranase and 1% mannase, and performing enzymolysis for 8 hr. And then heating to 50 ℃, keeping the temperature for 2h to inactivate enzyme, centrifuging the enzymolysis liquid to obtain supernatant, and drying the supernatant in a spray dryer, wherein the inlet air temperature is 130 ℃, and the outlet air temperature is 80 ℃ to obtain the powdery glutathione-enriched yeast extract.
Example 5
Yeast preparation was as in example 1.
Preparing the yeast into 6% (yeast dry matter content) yeast milk in a mixing tank, adjusting pH to 5.0, controlling temperature at 40 deg.C, and homogenizing under 10 MPa. Adding the above solution into a reaction tank, adjusting pH to 5.0, controlling temperature at 45 deg.C, adding 0.05% cellulose carbohydrase and 0.05% dextranase, and performing enzymolysis for 10 hr. And then heating to 55 ℃, keeping the temperature for 1h to inactivate enzyme, centrifuging the enzymolysis liquid to obtain supernatant, and drying the supernatant in a spray dryer at the air inlet temperature of 130 ℃ and the air outlet temperature of 80 ℃ to obtain the powdery glutathione-enriched yeast extract.
Example 6
Yeast preparation was as in example 1.
Preparing yeast into 6% (yeast dry matter content) yeast milk in a mixing tank, adjusting pH to 1.0, controlling temperature at 50 deg.C, and homogenizing under 100 MPa. Adding the above solution into a reaction tank, adjusting pH to 8.0, controlling temperature at 50 deg.C, adding 1% cellulose carbohydrase, 1% dextranase and 1% mannase, and performing enzymolysis for 3 hr. And then heating to 60 ℃, keeping the temperature for 2h to inactivate enzyme, centrifuging the enzymolysis liquid to obtain supernatant, and drying the supernatant in a spray dryer, wherein the inlet air temperature is 130 ℃, and the outlet air temperature is 80 ℃ to obtain the powdery glutathione-enriched yeast extract.
Example 7
Weighing 10g of glutathione-rich yeast extract, 8g of propylene glycol, 15g of acrylamide dimethyl ammonium taurate/VP copolymer, 3g of glycerol, 1g of carboxymethyl chitosan, 1g of hyaluronic acid, 0.1g of grapefruit essence and 3g of phenoxyethanol in the embodiment 3, adding the materials into 58.9g of water, and uniformly stirring to obtain the glutathione-rich yeast extract mask.
Comparative example 1
Yeast preparation was as in example 1.
Preparing yeast into 10% (yeast dry matter content) yeast milk in a blending tank, adjusting pH to 3.0, heating to 30 deg.C, homogenizing under 4 MPa. Adding the above solution into a reaction tank, adjusting pH to 6.0, maintaining the temperature at 40 deg.C, adding 1% cellulase and 1% dextranase, and performing enzymolysis for 3 hr. Then heating to 80 ℃, preserving the temperature for 0.5h to inactivate enzyme, and then centrifuging the enzymolysis liquid to obtain supernatant.
Comparative example 2
Yeast preparation was as in example 1.
Preparing 15% (yeast dry matter content) yeast milk from the obtained yeast in a mixing tank, adjusting pH to 7.0, cooling to 4 deg.C, and homogenizing under 5 MPa. Adding the above solution into a reaction tank, adjusting pH to 1.0, heating to 60 deg.C, adding 0.05% cellulase and 0.05% mannosidase, and performing enzymolysis for 15 hr. Then heating to 70 ℃, keeping the temperature for 3h to inactivate enzyme, then centrifuging the enzymolysis liquid to obtain supernatant, and then concentrating the supernatant by adopting a multi-effect evaporation process until the mass concentration of dry matters is 60% to obtain a pasty glutathione-enriched yeast extract, wherein the evaporation temperature is 65 ℃.
Comparative example 3
Yeast preparation was as in example 1.
Preparing 15% (yeast dry matter content) yeast milk from the above yeast in a mixing tank, adjusting pH to 2.0, controlling temperature at 10 deg.C, and homogenizing under 40 MPa. Adding the above solution into a reaction tank, adjusting pH to 2.0, controlling temperature at 30 deg.C, adding 0.05% cellulase and 0.03% dextranase, and performing enzymolysis for 12 hr. And then heating to 65 ℃, keeping the temperature for 1.5h to inactivate enzyme, centrifuging the enzymolysis liquid to obtain supernatant, and drying the supernatant in a spray dryer, wherein the inlet air temperature is 130 ℃, and the outlet air temperature is 80 ℃ to obtain the powdery yeast extract rich in glutathione.
Comparative example 4
Weighing 8g of propylene glycol, 15g of acrylamide dimethyl ammonium taurate/VP copolymer, 3g of glycerol, 1g of carboxymethyl chitosan, 1g of hyaluronic acid, 0.1g of grapefruit essence and 3g of phenoxyethanol, adding into 68.9g of water, and uniformly stirring to obtain the mask without the yeast extract.
Experimental example 1
The reduced glutathione content, protein content and DPPH radical scavenging rate of the yeast extracts prepared in examples 1-5 and comparative examples 1-3 were determined, and the inhibitory activity of the yeast extracts prepared in examples 1-5 and comparative examples 1-3, arbutin, vitamins and glutathione tyrosinase was determined.
1. Method for measuring content of reduced glutathione
The content of the reduced glutathione is determined by adopting the appendix A of the national standard GB/T35882-2018 'enriched Yeast'.
2. Method for measuring protein content
The total protein content is determined according to the third combustion method of GB/T5009.5-2016 protein determination in national food safety Standard food.
Method for measuring DPPH free radical clearance rate
Accurately transferring 10mg/mL reduced glutathione standard substance and 20 muL of yeast extract respectively, adding 280 muL of DPPH free radical absolute ethanol solution with the concentration of 65 mumol/L, reacting for 30min in a dark place at room temperature, measuring absorbance Ax at the wavelength of 517nm, and calculating the DPPH free radical clearance rate according to the following formula.
Clearance%0-AX)/A0*100％(1)；
Wherein: a. the0Blank control absorbance; a. theXIs the absorbance of the sample.
4. Determination method for inhibiting tyrosinase activity
Preparing 0.5mol/L sodium phosphate buffer solution with pH6.8; preparing 2.5 mmol/L-tyrosine solution; preparing 1.5mg/mL tyrosinase solution; the samples to be tested are dissolved by distilled water and prepared into five groups of samples of 0.625mg/mL, 1.250mg/mL, 2.500mg/mL, 5.000mg/mL and 10.000 mg/mL. During the test, the specific design is shown in table 2, five test tubes are taken and respectively added with 50 μ L of each sample to be tested, another test tube is taken and added with 50 μ L of sodium phosphate buffer solution (as standard control), then 100 μ L of sodium phosphate buffer solution and 50 μ L of tyrosinase solution are respectively and sequentially added, water bath is carried out at 30 ℃ for 10min, then 100 μ L of tyrosine solution is respectively added, the timing is immediately started, and the light absorption value at 475nm wavelength is measured when the reaction is carried out for 20 min. The inhibition of tyrosinase by the test samples was calculated using the following formula and an approximation of the median inhibitory concentration (IC50) was estimated from the concentration-enzyme inhibition curve.
Inhibition rate (A-B)/A100%
Wherein: a is the absorbance of the standard control and B is the absorbance of the test sample.
TABLE 2 composition of reaction solution in enzyme Activity measurement (unit,. mu.L)
| Components of reaction solution | Group 1 | 2 groups of | Group 3 | 4 groups of | 5 groups of | Standard control |
| Test sample | 50 | 50 | 50 | 50 | 50 | |
| Buffer solution | 100 | 100 | 100 | 100 | 100 | 150 |
| L-tyrosine solution | 100 | 100 | 100 | 100 | 100 | 100 |
| Tyrosinase solution | 50 | 50 | 50 | 50 | 50 | 50 |
| Total volume | 300 | 300 | 300 | 300 | 300 | 300 |
The results of the performance indexes of examples 1 to 6 and comparative examples 1 to 3 are shown in Table 3.
TABLE 3 Performance index results for examples 1-6 and comparative examples 1-3
As is clear from Table 3, the yeast extracts obtained in examples 1 to 6 each had a reduced glutathione content of 5% or more, a protein content of 35% or more, a DPPH radical scavenging rate of 15 to 20%, and a tyrosinase half maximal inhibitory concentration (IC50) of 0.3mg/mL or less. From the test results, examples 1, 3, 4 and 5 had better effects in terms of reduced glutathione content, protein content and tyrosinase half maximal inhibitory concentration (IC 50). Compared with examples 1-3, the content of the reduced glutathione in comparative examples 1-3 is less than 3%, which may be caused by the fact that the reduced glutathione cannot be better dissolved out of the cells due to the lower homogenizing pressure and the lower compound enzyme dosage, and the reduced glutathione is easily oxidized due to the higher enzymolysis temperature, which all result in the reduction of the final glutathione content and also influence the DPPH free radical clearance rate and the tyrosinase activity inhibition strength of the yeast extract.
The test results of the tyrosinase half inhibition concentration of the yeast extract, arbutin, vitamin C and glutathione show that the tyrosinase activity inhibition of the yeast extract is far higher than that of arbutin, equivalent to that of vitamin C and slightly lower than that of glutathione.
Experimental example 2
About 200g of apple was cut into two halves, and the mask prepared in comparative example 4 was laid on one half and the mask rich in glutathione yeast extract prepared in example 6 was laid on one half, and color change was observed after 20 min.
The results showed that the apple surface covered with the mask prepared in comparative example 4 was yellowish brown, while the apple surface covered with the mask rich in glutathione yeast extract prepared in example 6 was moist and yellowish. The facial mask containing the glutathione-enriched yeast extract has whitening and antioxidation effects.
In conclusion, the glutathione-rich yeast extract prepared by the invention has the reduced glutathione content of more than 5 percent, the protein content of more than 35 percent, the DPPH free radical clearance rate of 15-20 percent and the tyrosinase half inhibition concentration IC50 of less than or equal to 0.3mg/L, has medium antioxidant activity, can inhibit the dark state of pigment and the formation of free radicals caused by the overexpression of tyrosinase in organisms, and thus lightens the skin color and achieves the whitening and antioxidant effects.
The foregoing is considered as illustrative and not restrictive in character, and that various modifications, equivalents, and improvements made within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (13)
1. The glutathione-rich yeast extract is characterized by comprising reduced glutathione and protein, wherein the content of the reduced glutathione is more than or equal to 5 percent and the content of the protein is more than or equal to 35 percent based on the mass of dry matter of the yeast extract, the DPPH free radical clearance rate of the glutathione-rich yeast extract is 15-20 percent, and the tyrosinase half inhibition concentration IC50 of the glutathione-rich yeast extract is less than or equal to 0.3 mg/L.
2. The glutathione-rich yeast extract of claim 1, wherein the content of the reduced glutathione is more than or equal to 10 percent, the content of the protein is more than or equal to 40 percent, and the tyrosinase half-inhibitory concentration IC50 of the glutathione-rich yeast extract is less than or equal to 0.2mg/L based on the mass of the dry matter of the yeast extract.
3. The method for preparing glutathione-enriched yeast extract of claim 1 or 2, comprising the following steps:
(1) carrying out high-pressure homogenization and wall breaking on the glutathione-rich yeast raw material to obtain yeast cell wall milk;
(2) performing enzymolysis on the yeast cell wall milk obtained in the step (1) by using a complex enzyme, wherein the complex enzyme is two or more of cellulase, glucanase and mannanase;
(3) and (3) sequentially carrying out enzyme deactivation treatment and centrifugal separation on the zymolyte obtained in the step (2), and taking supernatant fluid to obtain the glutathione-enriched yeast extract.
4. The method according to claim 3, further comprising concentrating or drying the glutathione-enriched yeast extract to obtain a paste or powder product.
5. The method according to claim 3 or 4, wherein in step (1), the high-pressure homogeneous wall breaking is performed at a pH of 1.0-7.0 and a temperature of 4-50 ℃, preferably at a pH of 2.0-6.0 and a temperature of 10-40 ℃, and more preferably at a pressure of 5-100MPa, preferably 10-80 MPa.
6. The preparation method according to any one of claims 3 to 5, characterized in that, in the step (2), the compound enzyme is added in an amount of 0.1 to 3 percent, preferably 1 to 2 percent, based on the mass of the dry substance of the yeast cell wall milk.
7. The preparation method according to claim 6, wherein in the step (2), the cellulase is added in an amount of 0.5-1% and the glucanase is added in an amount of 0.5-1% by mass of the dry substance of the yeast cell wall milk; or the addition amount of the cellulase is 0.5-1%, and the addition amount of the mannosidase is 0.5-1%; or the addition amount of the glucanase is 0.5-1%, and the addition amount of the mannase is 0.5-1%.
8. The preparation method according to any one of claims 3 to 7, wherein in step (2), the temperature of the enzymolysis is 10 to 50 ℃, and the pH is 1.0 to 8.0, preferably, the temperature of the enzymolysis is 25 to 45 ℃, and the pH is 2.0 to 6.0, further preferably, the enzymolysis time is 2 to 15 hours, preferably 3 to 12 hours.
9. The method according to any one of claims 3 to 8, wherein in step (3), the enzyme deactivation operation is carried out at a temperature of 50 to 80 ℃ for 0.5 to 1.5 hours.
10. Use of the glutathione-enriched yeast extract of claim 1 or 2 or the glutathione-enriched yeast extract prepared by the preparation method of claims 3 to 9 for antioxidation.
11. Use of the glutathione-enriched yeast extract of claim 1 or 2 or the glutathione-enriched yeast extract prepared by the preparation method of claims 3 to 9 for preparing tyrosinase inhibitors.
12. Application of the glutathione-enriched yeast extract of claim 1 or 2 or the glutathione-enriched yeast extract prepared by the preparation method of claims 3-9 in the field of cosmetics, preferably whitening and antioxidant cosmetics.
13. The whitening and antioxidant mask is characterized by comprising the glutathione-rich yeast extract in the claim 1 or 2 or the glutathione-rich yeast extract prepared by the preparation method in the claims 3 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110664656.3A CN113350229A (en) | 2021-06-16 | 2021-06-16 | Glutathione-rich yeast extract, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110664656.3A CN113350229A (en) | 2021-06-16 | 2021-06-16 | Glutathione-rich yeast extract, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113350229A true CN113350229A (en) | 2021-09-07 |
Family
ID=77534435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110664656.3A Pending CN113350229A (en) | 2021-06-16 | 2021-06-16 | Glutathione-rich yeast extract, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113350229A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276942A (en) * | 2021-12-30 | 2022-04-05 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and application of glutathione yeast product |
| CN114806776A (en) * | 2022-04-26 | 2022-07-29 | 安琪酵母股份有限公司 | Biological antioxidant for fruit juice wine and preparation method and application thereof |
| CN115364128A (en) * | 2022-10-21 | 2022-11-22 | 北京本草源生物科技有限公司 | Zinc-containing composition and liver-protecting wine and application thereof |
| CN116445306A (en) * | 2023-06-15 | 2023-07-18 | 山东锦鲤生物工程有限公司 | Schizosaccharomyces pombe and its application in improving skin condition |
| CN116530659A (en) * | 2023-07-07 | 2023-08-04 | 北京衡美金叶营养健康科技有限公司 | Raw material composition with enhanced antioxidant function and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575578A (en) * | 2008-05-06 | 2009-11-11 | 安琪酵母股份有限公司 | Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof |
| CN102653722A (en) * | 2012-04-20 | 2012-09-05 | 湖北工业大学 | Preparation method of glutathione-enriched yeast |
| CN104757560A (en) * | 2014-08-11 | 2015-07-08 | 四川安益生物科技有限公司 | Glutathione yeast enzymolysis solution composition and preparation method thereof |
| CN105434321A (en) * | 2015-12-22 | 2016-03-30 | 温州任和教育科技有限责任公司 | Whitening freckle-removing mask and preparation method thereof |
-
2021
- 2021-06-16 CN CN202110664656.3A patent/CN113350229A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575578A (en) * | 2008-05-06 | 2009-11-11 | 安琪酵母股份有限公司 | Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof |
| CN102653722A (en) * | 2012-04-20 | 2012-09-05 | 湖北工业大学 | Preparation method of glutathione-enriched yeast |
| CN104757560A (en) * | 2014-08-11 | 2015-07-08 | 四川安益生物科技有限公司 | Glutathione yeast enzymolysis solution composition and preparation method thereof |
| CN105434321A (en) * | 2015-12-22 | 2016-03-30 | 温州任和教育科技有限责任公司 | Whitening freckle-removing mask and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276942A (en) * | 2021-12-30 | 2022-04-05 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and application of glutathione yeast product |
| WO2023125992A1 (en) * | 2021-12-30 | 2023-07-06 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and use of product |
| CN114806776A (en) * | 2022-04-26 | 2022-07-29 | 安琪酵母股份有限公司 | Biological antioxidant for fruit juice wine and preparation method and application thereof |
| CN115364128A (en) * | 2022-10-21 | 2022-11-22 | 北京本草源生物科技有限公司 | Zinc-containing composition and liver-protecting wine and application thereof |
| CN116445306A (en) * | 2023-06-15 | 2023-07-18 | 山东锦鲤生物工程有限公司 | Schizosaccharomyces pombe and its application in improving skin condition |
| CN116445306B (en) * | 2023-06-15 | 2023-09-01 | 山东锦鲤生物工程有限公司 | Schizosaccharomyces pombe and its application in improving skin condition |
| CN116530659A (en) * | 2023-07-07 | 2023-08-04 | 北京衡美金叶营养健康科技有限公司 | Raw material composition with enhanced antioxidant function and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113350229A (en) | Glutathione-rich yeast extract, and preparation method and application thereof | |
| EP2774993B1 (en) | Effective use of yeast and yeast extract residue | |
| KR101577079B1 (en) | Yeast autolysates | |
| RU2456347C2 (en) | Yeast extract containing inosinate disodium salt and guanylate disodium salt, extract production method | |
| JP4854199B2 (en) | Functional material obtained by re-fermenting liquefied sake lees | |
| CN114214387B (en) | Method for preparing schizophyllan and ergothioneine by co-fermentation of two strains | |
| CN108324633B (en) | Preparation method of brown rice fermentation stock solution for cosmetics and product thereof | |
| Kim et al. | High hydrostatic pressure treatment combined with enzymes increases the extractability and bioactivity of fermented rice bran | |
| CN111041059B (en) | Preparation method of Antarctic krill peptide with antioxidant activity | |
| CN114231354A (en) | High-efficiency beef tallow enzymolysis method | |
| CN110664726A (en) | Rice, tremella and mushroom mixed fermentation broth and preparation method thereof | |
| CN108094674A (en) | A kind of method that Freeze Drying Technique prepares soybean peptide | |
| Zhao et al. | In vitro antioxidant and antitumor activities of polysaccharides extracted from the mycelia of liquid-cultured Flammulina velutipes | |
| KR20100138664A (en) | A functional deer antlers product produced by two-step process and method for preparing thereof | |
| CN114672533B (en) | Method for removing bitter soybean peptide by utilizing aminopeptidase and alkaline protease in synergy | |
| CN105177095A (en) | Method for efficiently preparing antioxidative bioactive peptides of sea cucumbers | |
| CN115261414A (en) | Method for preparing tremella fermentation liquor by combining compound enzyme with microbial fermentation | |
| KR20160049251A (en) | The method of antioxidant peptides extracted from tuna fish heart | |
| CN107519105A (en) | One kind cleaning moisturizing spraying | |
| Lin et al. | Preparation of chaga medicinal mushroom, Inonotus obliquus− fermented rice using solid-state fermentation and its taste quality and antioxidant property | |
| CN111150045A (en) | Tea-flavored soy sauce rich in polyphenol and brewing method thereof | |
| KR102151742B1 (en) | Method for producing fermented rice bran with increased GABA content and fermented rice bran with increased GABA content produced by the same method | |
| WO2018225813A1 (en) | Water retentive agent for foods | |
| CN108244331A (en) | A kind of preparation method of very soluble soybean peptide | |
| CN115887341B (en) | Red rice enzymolysis extract and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |