CN113344593B - Meat product traceability management system based on DNA detection technology - Google Patents
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- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
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- G06Q30/018—Certifying business or products
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q50/00—Systems or methods specially adapted for specific business sectors, e.g. utilities or tourism
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- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
Abstract
The invention relates to the technical field of meat product traceability management, and discloses a meat product traceability management system based on a DNA detection technology, which comprises: the method comprises the steps that a cloud traceability server CTSmptms which is provided with meat product traceability management system server-side software and is used for storing a meat product traceability SNP marker library TL is operated, and a local traceability server LTSi which is provided with meat product traceability management system client-side software and is used for a meat product circulation link user Ui to upload detected meat product SNP markers Mj and obtain the meat product traceability SNP marker library TL is operated; the source tracing management method comprises the following steps: establishing a meat product traceability SNP marker library TL, carrying out SNP marker detection on a tissue sample of a received meat product to obtain a meat product SNP marker Mj, and comparing the detected meat product SNP marker Mj with the obtained traceability SNP marker TT in the meat product traceability SNP marker library TL. The invention solves the technical problem of how to realize accurate and reliable source tracing management of meat products based on a DNA detection technology.
Description
Technical Field
The invention relates to the technical field of meat product traceability management, in particular to a meat product traceability management system based on a DNA detection technology.
Background
Pork is the most important animal protein source in China, and the safety problem of the pork is generally concerned. The pork source tracing system is established, so that the traceable management of pork products can be realized, the detailed information related to products, including various links such as cultivation, transportation, slaughtering, cutting, sale and the like, is provided for consumers, and the strength of the government related departments in monitoring the quality safety of the meat products is greatly enhanced.
The label tracing technology is widely applied in China because of simple operation and low cost. However, the labels are confusing, easily dropped and lost, and once the pork product is separated from the carcass, it cannot be traced further. The DNA of an organism exists in the cells of any tissue thereof, and the DNA sequence of an individual has specificity (excluding monozygotic twins) and stability, so that the individual animal can be accurately identified by identifying the DNA characteristics of the individual without being affected by the division of the animal, and thus the DNA technology is used in the traceability of meat products.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a meat product traceability management system based on a DNA detection technology, and aims to solve the technical problem of how to realize accurate and reliable meat product traceability management based on the DNA detection technology.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme:
a meat product traceability management system based on a DNA detection technology comprises: the method comprises the steps that a cloud traceability server CTSmptms which is provided with meat product traceability management system server-side software and is used for storing a meat product traceability SNP marker library TL is operated, and a local traceability server LTSi which is provided with meat product traceability management system client-side software and is used for a meat product circulation link user Ui to upload detected meat product SNP markers Mj and obtain the meat product traceability SNP marker library TL is operated;
the meat product traceability management method based on the meat product traceability management system specifically comprises the following steps:
firstly, MPE of a meat product production enterprise acquires a traceability SNP marker TT of a meat product produced by the meat product production enterprise to obtain a meat product traceability SNP marker library TL, and then stores the meat product traceability SNP marker library TL in a cloud traceability server CTSmptms;
step two, a meat product circulation link user Ui firstly detects the SNP marker of the tissue sample of the meat product received by the user Ui to obtain a meat product SNP marker Mj, and then uploads the meat product SNP marker Mj to a local traceability server LTSi;
step three, if the local traceability server LTSi performs data interaction with the cloud traceability server CTSmptms for the first time, the local traceability server LTSi performs legal access authority registration on the meat product traceability management system;
if the local tracing server LTSi does not perform data interaction with the cloud tracing server CTSmptms for the first time, the local tracing server LTSi sends a request for obtaining the meat product tracing SNP marker library TL to the cloud tracing server CTSmptms;
step four, the meat product traceability management system verifies whether a user Ui in the meat product circulation link on the local traceability server LTSi has legal authority or not;
step five, if a meat product circulation link user Ui on the local traceability server LTSi has legal access authority, continuing to execute the next step;
otherwise, the tracing process is terminated immediately;
step six, allowing a meat product circulation link user Ui on a local traceability server LTSi to access by a cloud traceability server CTSmptms;
a system administrator SA on the cloud tracing server CTSmptms encrypts the meat product tracing SNP marker library TL and transmits an encrypted ciphertext Cx of the meat product tracing SNP marker library TL to the local tracing server LTSi;
step seven, the local traceability server LTSi decrypts the encrypted ciphertext Cx of the meat product traceability SNP marker library TL;
the local tracing server LTSi compares the detected meat product SNP marker Mj with the obtained tracing SNP marker TT in the meat product tracing SNP marker library TL;
step eight, if the meat product SNP marker Mj is matched with one traceability SNP marker TT in the meat product traceability SNP marker library TL, the meat product with the SNP marker Mj is proved to be produced by the meat product production enterprise MPE.
Further, the meat product traceability management system comprises: the system comprises a user registration module for managing local tracing server LTSi registration information, a user verification module for verifying the access authority of a special database PDsnp of a cloud tracing server CTSmptms, a storage module for storing meat product SNP markers Mj, an encryption module for encrypting and decrypting a meat product tracing SNP marker library TL, and a tracing module for comparing the meat product SNP markers Mj with tracing SNP markers TT in the meat product tracing SNP marker library TL.
Further, the specific step of the local tracing server LTSi performing the legal access right registration on the user registration module includes:
step S1, the user Ui on the local tracing server LTSi inputs the identity information IDui of the user into the user registration module;
step S2, the user registration module is in binary field F2 mUpper selection of an elliptic curve Ep(a,b)；
On the above-mentioned elliptic curve Ep(a, b) randomly selecting a point L as a base point, and then taking the elliptic curve Ep(a, b) disclosing a base point L to a user Ui on a local tracing server LTSi;
in step S3, the user Ui on the local tracing server LTSi selects one in the binary field F2 mS in binary field F2 mThe public key S above, and making S ═ sL true;
and then synchronously transmitting the public key S to a user registration module and a system administrator SA on the cloud tracing server CTSmptms.
Further, the specific step of verifying the access right of the user Ui on the local tracing server LTSi by the user verification module includes:
step S1, local tracingUser Ui on source server LTSi randomly selects one in binary field F2 mThe value m above, Q is calculated to mL, and Q is plotted on an elliptic curve Ep(a, b) and thereafter transmitting Q to the user authentication module;
in step S2, the user authentication module is automatically generated in binary field F2 mThe random number N is transmitted to a user Ui on the local tracing server LTSi;
step S3, the user Ui on the local tracing server LTSi calculates E as m + Qs, and transmits E to the user authentication module;
in step S4, the user verification module verifies whether the equation EL ═ Q + NS is true, and if true, it indicates that the user Ui on the local tracing server LTSi has a legal access right.
Further, the specific steps of the system administrator SA on the cloud tracing server ctsmsmptms to encrypt the meat product tracing SNP marker library TL on the encryption module include:
step S1, the system administrator SA on the cloud tracing server CTSmptms encodes the meat product tracing SNP marker library TL into E on the encryption modulepA point X on (a, b);
in step S2, the encryption module generates a key in binary field F2 mN, and X is satisfied at the elliptic curve Ep(a, b);
in step S3, the encryption module calculates the ciphertext Cx ═ X + nS of the meat product tracing SNP marker library TL.
Further, the concrete steps of the user Ui in the meat product circulation link decrypting the encrypted ciphertext Cx of the meat product traceability SNP marker library TL on the encryption module include:
step S1, solving equation Cx ═ nL (1+ S) according to key S of local tracing server LTSi to obtain n;
step S2, obtaining X by solving equation Cx ═ X + nS, based on n;
and step S3, obtaining the meat product traceability SNP marker library TL according to the X and the coding relation between the meat product traceability SNP marker library TL and the X.
Further, the cloud tracing server ctsmsmptms is configured with a dedicated database PDsnp for storing the meat product tracing SNP marker library TL.
(III) advantageous technical effects
Compared with the prior art, the invention has the following beneficial technical effects:
according to the invention, a meat product traceability SNP marker library TL is established through MPE of a meat product production enterprise, SNP marker detection is carried out on a tissue sample received by a meat product through a meat product circulation link user Ui, a meat product SNP marker Mj is obtained, and the detected meat product SNP marker Mj is compared with the obtained traceability SNP marker TT in the meat product traceability SNP marker library TL, so that accurate and reliable traceability of the meat product is realized.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A meat product traceability management system based on a DNA detection technology comprises: the method comprises the steps that a cloud traceability server CTSmptms which is provided with meat product traceability management system server software and is constructed in a traceability management system of a meat product production enterprise is installed and operated, and the cloud traceability server CTSmptms is provided with a special database PDsnp for storing a meat product traceability SNP marker library TL;
the meat product traceability management system further comprises: the method comprises the steps that client software of a meat product traceability management system is installed and operated, and a local traceability server LTSi (i ═ 1,2, …, n) which is used for uploading detected meat product SNP markers Mj (j ═ 1,2, …, m) by a meat product circulation link user Ui (i ═ 1,2, …, n) and acquiring a meat product traceability SNP marker library TL through accessing a special database PDsnp is installed and operated;
the meat product circulation link user Ui performs data interaction between meat product traceability management system client software running on a local traceability server LTSi and meat product traceability management system server software running on a cloud traceability server CTSmptms;
the meat product traceability management method based on the meat product traceability management system specifically comprises the following steps:
firstly, MPE of a meat product production enterprise acquires a meat product produced by the meat product production enterprise through a tracing SNP marker TT to obtain a meat product tracing SNP marker library TL, and then stores the meat product tracing SNP marker library TL in a special database PDsnp of a cloud tracing server CTSmptms;
step two, a meat product circulation link user Ui firstly detects the SNP marker of the tissue sample of the meat product received by the user Ui to obtain a meat product SNP marker Mj, and then uploads the meat product SNP marker Mj to a local traceability server LTSi;
step three, the local traceability server LTSi registers legal access authority on the meat product traceability management system;
the local tracing server LTSi sends a request for accessing a special database PDsnp of the cloud tracing server CTSmptms and acquiring a meat product tracing SNP marker library TL to the cloud tracing server CTSmptms;
step four, the meat product traceability management system verifies whether a meat product circulation link user Ui on the local traceability server LTSi has the authority of accessing a special database PDsnp of the cloud traceability server CTSmptms;
step five, if a meat product circulation link user Ui on the local traceability server LTSi has legal access authority, continuing to execute the next step;
on the contrary, if a meat product circulation link user Ui on the local traceability server LTSi does not have the right to access the special database PDsnp, the traceability process is immediately terminated;
step six, allowing a meat product circulation link user Ui on a local traceability server LTSi to access a special database PDsnp by a cloud traceability server CTSmptms;
a system administrator SA on the cloud tracing server CTSmptms encrypts the meat product tracing SNP marker library TL and transmits an encrypted ciphertext Cx of the meat product tracing SNP marker library TL to the local tracing server LTSi;
step seven, the local traceability server LTSi decrypts the encrypted ciphertext Cx of the meat product traceability SNP marker library TL;
the local tracing server LTSi compares the detected meat product SNP marker Mj with the obtained tracing SNP marker TT in the meat product tracing SNP marker library TL;
step eight, if the meat product SNP marker Mj is matched with a certain traceability SNP marker TT in the meat product traceability SNP marker library TL, the meat product with the SNP marker Mj is proved to be produced by the meat product production enterprise MPE;
on the contrary, if the meat product SNP marker Mj is not matched with any traceability SNP marker TT in the meat product traceability SNP marker library TL, the meat product with the SNP marker Mj is proved not to be produced by the meat product production enterprise MPE;
thereby realizing accurate and reliable tracing of the meat products;
further, the meat product traceability management system comprises: the system comprises a user registration module for managing local tracing server LTSi registration information, a user verification module for verifying the access authority of a special database PDsnp of a cloud tracing server CTSmptms, a storage module for storing meat product SNP markers Mj, an encryption module for encrypting and decrypting a meat product tracing SNP marker library TL, and a tracing module for comparing the meat product SNP markers Mj with tracing SNP markers TT in the meat product tracing SNP marker library TL;
the tracing module performs mutual data interaction with the encryption module and the storage module respectively, the encryption module performs mutual data interaction with the user registration module, and the user registration module performs mutual data interaction with the user verification module;
further, the specific steps of the local tracing server LTSi for performing legal access authority registration on the user registration module of the meat product tracing management system include:
step S1, the user Ui on the local tracing server LTSi inputs the identity information IDui of the user into the user registration module;
step S2, the user registration module is in binary field F2 mUpper selection of an elliptic curve Ep(a,b)；
On the above-mentioned elliptic curve Ep(a, b) randomly selecting a point L as a base point, and then taking the elliptic curve Ep(a, b) disclosing a base point L to a user Ui on a local tracing server LTSi;
in step S3, the user Ui on the local tracing server LTSi selects one in the binary field F2 mS in binary field F2 mThe public key S above, and making S ═ sL true;
then synchronously transmitting the public key S to a user registration module and a system administrator SA on a cloud tracing server CTSmptms;
further, the specific step of verifying the access authority of the user Ui on the local tracing server LTSi by the user verification module of the meat product tracing management system includes:
step S1, the user Ui on the local tracing server LTSi randomly selects one in the binary field F2 mThe value m above, Q is calculated to mL, and Q is plotted on an elliptic curve Ep(a, b) and thereafter transmitting Q to the user authentication module;
in step S2, the user authentication module is automatically generated in binary field F2 mThe random number N is transmitted to a user Ui on the local tracing server LTSi;
step S3, the user Ui on the local tracing server LTSi calculates E as m + Qs, and transmits E to the user authentication module;
step S4, the user verification module verifies whether the equation EL ═ Q + NS is true, and if true, it indicates that the user Ui on the local tracing server LTSi has a legal access right;
otherwise, the user Ui on the local tracing server LTSi is an illegal user;
further, the specific steps of the system administrator SA on the cloud tracing server ctsmsmptms to encrypt the meat product tracing SNP marker library TL on the encryption module of the meat product tracing management system include:
step S1, the system administrator SA on the cloud tracing server CTSmptms encodes the meat product tracing SNP marker library TL into E on the encryption modulepA point X on (a, b);
in step S2, the encryption module generates a key in binary field F2 mN, and X is satisfied at the elliptic curve Ep(a, b);
step S3, the encryption module calculates the ciphertext Cx of the meat product traceability SNP marker library TL as X + nS;
further, the specific steps of the local tracing server LTSi decrypting the encrypted ciphertext Cx of the meat product tracing SNP marker library TL on the encryption module include:
step S1, solving equation Cx ═ nL (1+ S) according to key S of local tracing server LTSi to obtain n;
step S2, obtaining X by solving equation Cx ═ X + nS, based on n;
step S3, obtaining a meat product traceability SNP marker library TL according to the X and the coding relation between the meat product traceability SNP marker library TL and the X;
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A meat product traceability management system based on a DNA detection technology is characterized by comprising: the method comprises the steps that a cloud traceability server CTSmptms which is provided with meat product traceability management system server-side software and is used for storing a meat product traceability SNP marker library TL is operated, and a local traceability server LTSi which is provided with meat product traceability management system client-side software and is used for a meat product circulation link user Ui to upload detected meat product SNP markers Mj and obtain the meat product traceability SNP marker library TL is operated;
the meat product traceability management method based on the meat product traceability management system specifically comprises the following steps:
firstly, MPE of a meat product production enterprise acquires a traceability SNP marker TT of a meat product produced by the meat product production enterprise to obtain a meat product traceability SNP marker library TL, and then stores the meat product traceability SNP marker library TL in a cloud traceability server CTSmptms;
step two, a meat product circulation link user Ui firstly detects the SNP marker of the tissue sample of the meat product received by the user Ui to obtain a meat product SNP marker Mj, and then uploads the meat product SNP marker Mj to a local traceability server LTSi;
step three, if the local traceability server LTSi performs data interaction with the cloud traceability server CTSmptms for the first time, the local traceability server LTSi performs legal access authority registration on the meat product traceability management system;
if the local tracing server LTSi does not perform data interaction with the cloud tracing server CTSmptms for the first time, the local tracing server LTSi sends a request for obtaining the meat product tracing SNP marker library TL to the cloud tracing server CTSmptms;
step four, the meat product traceability management system verifies whether a user Ui in the meat product circulation link on the local traceability server LTSi has legal authority or not;
step five, if a meat product circulation link user Ui on the local traceability server LTSi has legal access authority, continuing to execute the next step;
otherwise, the tracing process is terminated immediately;
step six, allowing a meat product circulation link user Ui on a local traceability server LTSi to access by a cloud traceability server CTSmptms;
a system administrator SA on the cloud tracing server CTSmptms encrypts the meat product tracing SNP marker library TL and transmits an encrypted ciphertext Cx of the meat product tracing SNP marker library TL to the local tracing server LTSi;
step seven, the local traceability server LTSi decrypts the encrypted ciphertext Cx of the meat product traceability SNP marker library TL;
the local tracing server LTSi compares the detected meat product SNP marker Mj with the obtained tracing SNP marker TT in the meat product tracing SNP marker library TL;
step eight, if the meat product SNP marker Mj is matched with one traceability SNP marker TT in the meat product traceability SNP marker library TL, the meat product with the SNP marker Mj is proved to be produced by the meat product production enterprise MPE.
2. The meat product traceability management system based on DNA detection technology as claimed in claim 1, characterized in that the meat product traceability management system comprises: the system comprises a user registration module for managing local tracing server LTSi registration information, a user verification module for verifying the access authority of a special database PDsnp of a cloud tracing server CTSmptms, a storage module for storing meat product SNP markers Mj, an encryption module for encrypting and decrypting a meat product tracing SNP marker library TL, and a tracing module for comparing the meat product SNP markers Mj with tracing SNP markers TT in the meat product tracing SNP marker library TL.
3. The meat product traceability management system based on DNA detection technology as claimed in claim 2, wherein the specific steps of the local traceability server LTSi performing legal access authority registration on the user registration module comprise:
step S1, the user Ui on the local tracing server LTSi inputs the identity information IDui of the user into the user registration module;
step S2, the user registration module is in binary field F2 mUpper selection of an elliptic curve Ep(a,b)；
On the above-mentioned elliptic curve Ep(a, b) randomly selecting a point L as a base point, and then taking the elliptic curve Ep(a, b) disclosing a base point L to a user Ui on a local tracing server LTSi;
in step S3, the user Ui on the local tracing server LTSi selects one in the binary field F2 mS in binary field F2 mThe public key S above, and making S ═ sL true;
and then synchronously transmitting the public key S to a user registration module and a system administrator SA on the cloud tracing server CTSmptms.
4. The meat product traceability management system based on DNA detection technology as claimed in claim 3, wherein the specific step of the user verification module verifying the access authority of the user Ui on the local traceability server LTSi comprises:
step S1, the user Ui on the local tracing server LTSi randomly selects one in the binary field F2 mThe value m above, Q is calculated to mL, and Q is plotted on an elliptic curve Ep(a, b) and thereafter transmitting Q to the user authentication module;
in step S2, the user authentication module is automatically generated in binary field F2 mThe random number N is transmitted to a user Ui on the local tracing server LTSi;
step S3, the user Ui on the local tracing server LTSi calculates E as m + Qs, and transmits E to the user authentication module;
in step S4, the user verification module verifies whether the equation EL ═ Q + NS is true, and if true, it indicates that the user Ui on the local tracing server LTSi has a legal access right.
5. The meat product traceability management system based on DNA detection technology as claimed in claim 4, wherein the specific steps of the system administrator SA on the cloud traceability server CTSmptms to encrypt the meat product traceability SNP marker library TL on the encryption module comprise:
step S1, the system administrator SA on the cloud tracing server CTSmptms encodes the meat product tracing SNP marker library TL into E on the encryption modulepA point X on (a, b);
in step S2, the encryption module generates a key in binary field F2 mN, and X is satisfied at the elliptic curve Ep(a, b);
in step S3, the encryption module calculates the ciphertext Cx ═ X + nS of the meat product tracing SNP marker library TL.
6. The meat product traceability management system based on the DNA detection technology as claimed in claim 5, wherein the concrete steps of the meat product circulation link user Ui decrypting the encrypted ciphertext Cx of the meat product traceability SNP marker library TL on the encryption module comprise:
step S1, solving equation Cx ═ nL (1+ S) according to key S of local tracing server LTSi to obtain n;
step S2, obtaining X by solving equation Cx ═ X + nS, based on n;
and step S3, obtaining the meat product traceability SNP marker library TL according to the X and the coding relation between the meat product traceability SNP marker library TL and the X.
7. The meat product traceability management system based on DNA detection technology as claimed in claim 6, wherein the cloud traceability server CTSmptms is configured with a dedicated database PDsnp for storing the meat product traceability SNP marker library TL.
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