CN113341152A - Application of RPS9 protein in prediction of good response of crab eating monkey to superovulation - Google Patents
Application of RPS9 protein in prediction of good response of crab eating monkey to superovulation Download PDFInfo
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Abstract
The invention relates to an application of RPS9 protein in predicting good response of crab-eating monkey superovulation, belonging to the technical field of superovulation, wherein the biological information analysis is carried out on blood sample protein of the superovulation successful group on day 1, the superovulation successful group on day 5, the superovulation failed group on day 1 and the superovulation failed group on day 5 by a proteomic method to obtain differential protein among the groups. From the protein level studies, studies were conducted to reveal the changes in protein expression in the blood of superovulated monkeys. In the cynomolgus monkey superovulation, the RPS9 protein was expressed on day 5 in the superovulation successful group, but not in the day 5 superovulation failure group. It was suggested that down-regulation of PRS9 in the superovulation failure group affected activation of MAPK signaling pathway and NK-kb pathway, thereby causing apoptosis, cell cycle arrest and inhibition of proliferation. Downregulation of PRS9 expression may lead to activation of p53, resulting in inhibition of cell proliferation, affecting follicular formation, leading to failure of the superovulation response. Therefore, the RPS9 protein becomes a new molecule for predicting good response of crab eating monkey superovulation and has practical application value.
Description
Technical Field
The invention relates to the technical field of superovulation, in particular to application of RPS9 protein in predicting good response of cynomolgus monkey superovulation.
Background
Superovulation is a technique of stimulating the ovaries of female animals by using exogenous hormones to promote the simultaneous growth and development of multiple follicles in the ovaries, and finally obtaining multiple mature oocytes. Cynomolgus monkeys have only one dominant follicle in the natural menstrual cycle to mature and ovulate. If the superovulation technology is used in the menstrual period, the number of oocytes per ovulation can be increased from the original 1 to 10-20 or more. Mature oocytes are important materials for the development of human diseases, genetic engineering and embryo engineering studies. However, the number of oocytes that can be obtained in a natural cycle is very small, and thus the experimental requirements cannot be met, and the required cycle is too long. The superovulation can fully explore the reproductive potential of female animals, and can ensure that a large number of oocytes in ovaries grow, develop and mature. Therefore, obtaining a large number of mature oocytes by superovulation is an effective and feasible approach.
Although more mature oocytes than normal natural cycle are obtained in different superovulation schemes, there are large differences in the number of oocytes obtained from different animals. Relatively speaking, the superovulation effect of obese cynomolgus monkeys with the weight more than 5kg and the superovulation effect of cynomolgus monkeys with the weight less than 3kg are not ideal, and the number of mature oocytes in superovulation of the cynomolgus monkeys with the weight of 3-5 kg is large. In repeated superovulation, if the macaque has poor ovarian response during the first superovulation, the superovulation effect is not ideal during the subsequent superovulation. If the macaque with better ovarian response during the last superovulation is selected for repeated superovulation, the quantity and quality of the oocytes obtained in the next superovulation are more ideal. In addition, macaques with seasonal reproduction have significantly lower numbers of mature oocytes obtained by superovulation in the non-reproductive season than in the reproductive season. In addition, menstrual cycle disturbances, diarrhea and the presence of undegraded corpus luteum in the ovaries can also lead to undesirable effects of superovulation.
The ribosomal protein S9(RPS9) gene encodes a ribosomal protein that is a component of the 40S subunit and is also an essential ribosomal protein for assembly of the 30S ribosomal protein complex. The protein belongs to the S4P family of ribosomal proteins, is located in cytoplasm, and is involved in ribosome production. During the bovine lactation cycle, the mammary gland is characterized by cellular and molecular changes compared to changes in the ovaries during the oestrus cycle. RPS9 was stably expressed in bovine mammary glands and served as a suitable reference gene. The bovine ovary contains at its various locations a large number of heterogeneous cell populations that are affected at the morphological and molecular level by the estrous cycle and pregnancy. H3F3B and RPS9 were used as the best reference genes for healthy bovine ovary normalization. At present, no research on the interaction between RPS9 protein and superovulation is reported, and the interaction between RPS9 and ovaries, oocytes and the like in the superovulation process is not clear.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention firstly provides the application of the RPS9 protein in predicting the good response of the crab eating monkey to superovulation.
The purpose of the invention is realized by the following technical scheme:
the RPS9 protein is used for predicting the good response of the super ovulation of the cynomolgus monkey.
Superovulation is always the key point of research on assisted reproductive technologies, and the number, maturity and development potential of oocytes obtained in superovulation are very important. The cynomolgus monkey can obtain ten or even dozens of mature MII-stage oocytes in normal superovulation, however, after repeated superovulation, part of the cynomolgus monkeys begin to respond in superovulation, can obtain only a small amount of GV-stage eggs, and perform poorly in subsequent superovulation. Although the ovary stores a large number of primordial follicles, it cannot be efficiently harvested by superovulation. Although numerous studies on superovulation have confirmed the presence of antibodies, body weight, season, age, etc. influencing factors in superovulation, superovulation is a relative regulation between gonadotropins and oocytes and is a complex dynamic process. Therefore, the mechanism affecting superovulation has not been elucidated and effective solutions have not been proposed. Since proteins are the final actors in life activities, the superovulation process is also the result of the relative action of a large number of proteins in the body.
In this experiment, a DIA non-standard quantitative technique was used, which divides the entire scan range of the mass spectrum into several windows and performs high-speed, cyclic selection, fragmentation and detection of all ions in each window. Therefore, compared with the traditional DDA technology, DIA can collect all fragment information in a sample, greatly improve data utilization rate, have few missing values, and have higher detection rate of low-abundance proteins. In the research, blood samples are grouped according to the superovulation result, and the blood samples subjected to superovulation of 6 cynomolgus monkeys are analyzed through a proteomics method to identify 1550 proteins in total, wherein the biological information analysis is performed on the sample proteins of the superovulation successful group on day 1, the superovulation successful group on day 5, the superovulation failed group on day 1 and the superovulation failed group on day 5, so as to obtain the difference proteins among the groups. From the protein level studies, studies were conducted to reveal the changes in blood protein expression during superovulation. Attempts to screen out related differential proteins influencing the quantity and quality of oocytes in superovulation provide possible molecular markers for further improving superovulation schemes and predicting superovulation results.
It was found from the protein qualitative analysis that the RPS9 protein was expressed on day 5 in the superovulation successful group, but not in the superovulation failure group on day 5. It was suggested that down-regulation of PRS9 in the superovulation failure group affected activation of MAPK signaling pathway and NK-kb pathway, thereby causing apoptosis, cell cycle arrest and inhibition of proliferation. Downregulation of PRS9 expression may lead to activation of p53, resulting in inhibition of cell proliferation, affecting follicular formation, leading to failure of the superovulation response.
Preferably, the RPS9 protein is used as a positive regulator for predicting the good response of the super ovulation of the cynomolgus monkey.
Thus, in particular predicting the success or failure of cynomolgus superovulation, it can be predicted by detecting the presence or absence of RPS9 protein in blood samples from day 1 to day 5 of cynomolgus superovulation.
Of course, when the cynomolgus monkey superovulates, the hormone may be administered according to the existing scheme, which includes but is not limited to the following three:
the first method comprises the following steps: female cynomolgus monkeys were subcutaneously injected with 3.75mg of GnRH agonist on day 1 of menstruation. Two to three weeks later, female cynomolgus monkeys injected 75IU/kg rhFSH subcutaneously, three times every 72 hours, and 60 hours after the last injection, 1200IU hCG was intravenously injected.
And the second method comprises the following steps: the female monkey was administered with 60IU of rhFSH twice daily for 6 consecutive days, 60IU of rhFSH twice daily for 3 consecutive days, and then 5 hours later with 1000IU of hCG intravenously. To suppress premature ovulation, 0.25mg of GnRH inhibitor was injected daily starting on day 1 of stimulation of rhFSH and continuing until the day of hCG.
And the third is that: on day 1 of menstruation, female cynomolgus monkeys were injected subcutaneously with 3.75mg of GnRH agonist. After two to three weeks, follicular growth was stimulated by either the a or B method. The method A comprises the following steps: 25IU/kg rhFSH was dissolved in glycerol/physiological saline (1:1) and injected subcutaneously once a day for 9 days, and after 36h, 1200IU hCG was injected intravenously. The method B comprises the following steps: female cynomolgus monkeys injected 75IU/kg hFSH subcutaneously every 3 days, and after 60h interval for the last treatment, 1200IU hCG intravenously.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, through a proteomics method, biological information analysis is carried out on the sample proteins of the superovulation successful group on day 1, the superovulation successful group on day 5, the superovulation failed group on day 1 and the superovulation failed group on day 5, so as to obtain the differential proteins among the groups. From the protein level studies, studies were conducted to reveal the changes in blood protein expression during superovulation. Attempts to screen out related differential proteins influencing the quantity and quality of oocytes in superovulation provide possible molecular markers for further improving superovulation schemes and predicting superovulation results.
In the cynomolgus monkey superovulation, the RPS9 protein was expressed on day 5 in the superovulation successful group, but not in the day 5 superovulation failure group. It was suggested that down-regulation of PRS9 in the superovulation failure group affected activation of MAPK signaling pathway and NK-kb pathway, thereby causing apoptosis, cell cycle arrest and inhibition of proliferation. Downregulation of PRS9 expression may lead to activation of p53, resulting in inhibition of cell proliferation, affecting follicular formation, leading to failure of the superovulation response.
The prediction provides a new method for predicting the good response of the crab eating monkey to the superovulation, and has practical application value.
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FIG. 1 is a flow chart of the proteomics experimental procedures and results analysis of the present invention;
FIG. 2 shows the results of quantitative analysis of differential protein; wherein FIG. 2a is a histogram of the differential protein; FIG. 2b is a Wien diagram of differential proteins and total number of identified proteins.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The test methods used in the following experimental examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
One, superovulation experimental process
Physiological status of adult female cynomolgus monkeys in the target monkey cohort was observed daily and cage and monkey number registration was made on day 1 where menses were found to occur. On days 1-4 of menstruation, venous blood sample collection, B-type ultrasonic image examination of ovary and uterus and color ultrasonic image examination of ovary and uterus are carried out on cynomolgus monkeys in menstrual period. Meanwhile, the intramuscular injection of rhFSH was started on the evening of the day the menstrual blood sample was collected, and the day was recorded as day 1 of the superovulation. rhFSH was injected once a day, in the morning and in the evening, starting on the second day. On day 5 of injection of rhFSH, blood sample collection was again performed. The needle was stopped after completion of the intramuscular injection of rhFSH on the 9 th morning of superovulation. The superovulation cynomolgus monkeys were given an intramuscular injection of hCG once on the evening of day 10 of superovulation. Finally, blood sample collection and laparoscopic ovum retrieval were performed 36 hours after rhCG injection and on the 12 morning of superovulation.
Proteomics detection and result analysis
The blood samples were divided into 4 groups, which were day 1 in the super-exclusion failure group, day 5 in the super-exclusion failure group, day 1 in the super-exclusion success group, and day 5 in the super-exclusion success group, respectively. The 4 groups of plasma samples were subjected to subsequent work of proteomics, and the 4 groups of proteins were analyzed in comparison with each other until protein data was generated. The proteomics method selects DIA non-labeled quantitative proteomics technology to complete analysis. Under the data independent acquisition mode, a large amount of proteome coverage can be provided, and simultaneously, a large amount of proteins in each sample can be accurately and highly repeatable quantified, so that the quantitative analysis platform is an ideal proteome quantitative platform for differential proteome qualitative analysis or mass samples. The specific experimental process and the information analysis process are shown in fig. 1. The protein sequences finally identified in proteomics were all derived from the UniProt protein database, NCBI and Ensembl gene annotation system, and were done by the Spectronaut software, with the differential analysis done by the RStudio software.
The data results of four groups of blood sample proteomics are screened and integrated, and the study is carried out from protein characterization and differential analysis.
The experimental results are as follows: blood proteins of female cynomolgus monkeys with poor superovulation response on day 1 and day 5 were designated as Treated1 group (T1) and Treated 2 group (T2), respectively, and blood proteins of female cynomolgus monkeys with good superovulation response on day 1 and day 5 were designated as Treated 3 group (T3) and Treated 4 group (T4), respectively. The first part is to compare and analyze the differential protein between the super-exclusion failure group T1 and T2 and the differential protein between the super-exclusion success groups T3 and T4, and compare the change of the differential protein in the super-exclusion process between the super-exclusion failure group and the super-exclusion success group; the second part is to analyze the differential protein of the superovulation failure group T1 and the superovulation success group T3 and compare the difference of the blood protein of the superovulation failure group and the superovulation success group in the menstrual period; and in the third part, the differential proteins of the superovulation failure group T2 and the superovulation success group T4 are analyzed, and the differential proteins of blood in the superovulation process between the superovulation success group and the superovulation failure group are compared. Wherein each portion of the differential protein is to be analyzed in terms of Gene Ontology (GO), pathway enrichment analysis (KEGG), interactions, etc. of the differential protein.
As shown in fig. 2a, the quantitative analysis of differential proteins revealed that T1 had a total of 56 differential proteins compared to T2, of which 52 were up-regulated and 4 were down-regulated.
Since the differential protein analysis results were performed on the differential levels of proteins present in all 4 groups of samples, 55 proteins were not detected in some of the 4 groups of samples, as shown in fig. 2b, in addition to 146 differential proteins among the identified proteins, including 60S ribosomal protein L26(60S ribosomal protein L26, RPS9), 40S ribosomal protein S9(40S ribosomal protein S9, RPS9), Catechol O-methyltransferase (COMT). Among them, RPS9 protein was not detected on day 5 in the superovulation failure group, but was present in all other groups. In the GO analysis, the RPS9 protein is mainly involved in the processes of translation, RNA combination and the like. In KEGG analysis, RPS9 protein is mainly involved in the ribosome signaling pathway. It has been shown that interference with ribosome biogenesis causes ribosome stress, and a group of Ribosomal Proteins (RPs) appear as key mediators of the P53 signaling pathway. Therefore, in the cynomolgus monkey superovulation, the RPS9 protein was expressed on day 5 in the superovulation successful group, but not in the day 5 superovulation failure group. It was suggested that down-regulation of PRS9 in the superovulation failure group affected activation of MAPK signaling pathway and NK-kb pathway, thereby causing apoptosis, cell cycle arrest and inhibition of proliferation. Downregulation of PRS9 expression may lead to activation of p53, resulting in inhibition of cell proliferation, affecting follicular formation, leading to failure of the superovulation response.
Claims (2)
- The application of RPS9 protein in predicting the good response of the super ovulation of cynomolgus monkeys.
- 2. The use according to claim 1, wherein the RPS9 protein is used as a positive regulator in the prediction of good response to ovarian hyperstimulation in cynomolgus monkeys.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020058880A1 (en) * | 2000-11-12 | 2002-05-16 | Tanita Corporation | Method and instrument for estimating condition of growing ovum |
| CN1517362A (en) * | 2002-06-28 | 2004-08-04 | 上海博德基因开发有限公司 | New Polypeptide-human ribosome protein s9 containing ATP/GTP conjugated structure domain and polynucleotide coding said polypetide |
| CN1662643A (en) * | 2002-05-28 | 2005-08-31 | 贝克顿·迪金森公司 | Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells |
| CN103169699A (en) * | 2011-12-22 | 2013-06-26 | 中国农业大学 | Application of letrozole (LE) in preparation of drug used for causing adult cattle to excessively ovulate |
| CN104839085A (en) * | 2015-05-12 | 2015-08-19 | 华南农业大学 | Method for inducing hen to ovulate regularly |
| CN109825564A (en) * | 2019-04-05 | 2019-05-31 | 南京优而生物科技发展有限公司 | A kind of SLC2A1 expression is preparing the application in oocyte fertilization ability detection kit |
| CN109913531A (en) * | 2019-03-29 | 2019-06-21 | 武汉市农业科学院 | Ox propagating system and the screening of embryonic tissue quantitative PCR detection reference gene and application |
| CN109913533A (en) * | 2019-03-29 | 2019-06-21 | 武汉市农业科学院 | Pregnant cattle uterus mound mucous membrane fluorogenic quantitative detection reference gene screening technique and application |
| CN109971829A (en) * | 2019-03-29 | 2019-07-05 | 武汉市农业科学院 | The reference gene screening technique of pregnant dairy cows fallopian tubal fluorogenic quantitative detection and application |
| CN110852357A (en) * | 2019-10-24 | 2020-02-28 | 开望(杭州)科技有限公司 | Ovulation test paper category detection method |
| CN111630173A (en) * | 2017-10-19 | 2020-09-04 | 库瑞瓦格股份公司 | Novel artificial nucleic acid molecules |
| CN112410439A (en) * | 2020-12-11 | 2021-02-26 | 华南农业大学 | Application of chi-miR-128-3p as goat follicle maturation miRNA marker |
-
2021
- 2021-04-27 CN CN202110462106.3A patent/CN113341152B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020058880A1 (en) * | 2000-11-12 | 2002-05-16 | Tanita Corporation | Method and instrument for estimating condition of growing ovum |
| CN1662643A (en) * | 2002-05-28 | 2005-08-31 | 贝克顿·迪金森公司 | Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells |
| CN1517362A (en) * | 2002-06-28 | 2004-08-04 | 上海博德基因开发有限公司 | New Polypeptide-human ribosome protein s9 containing ATP/GTP conjugated structure domain and polynucleotide coding said polypetide |
| CN103169699A (en) * | 2011-12-22 | 2013-06-26 | 中国农业大学 | Application of letrozole (LE) in preparation of drug used for causing adult cattle to excessively ovulate |
| CN104839085A (en) * | 2015-05-12 | 2015-08-19 | 华南农业大学 | Method for inducing hen to ovulate regularly |
| CN111630173A (en) * | 2017-10-19 | 2020-09-04 | 库瑞瓦格股份公司 | Novel artificial nucleic acid molecules |
| CN109913531A (en) * | 2019-03-29 | 2019-06-21 | 武汉市农业科学院 | Ox propagating system and the screening of embryonic tissue quantitative PCR detection reference gene and application |
| CN109913533A (en) * | 2019-03-29 | 2019-06-21 | 武汉市农业科学院 | Pregnant cattle uterus mound mucous membrane fluorogenic quantitative detection reference gene screening technique and application |
| CN109971829A (en) * | 2019-03-29 | 2019-07-05 | 武汉市农业科学院 | The reference gene screening technique of pregnant dairy cows fallopian tubal fluorogenic quantitative detection and application |
| CN109825564A (en) * | 2019-04-05 | 2019-05-31 | 南京优而生物科技发展有限公司 | A kind of SLC2A1 expression is preparing the application in oocyte fertilization ability detection kit |
| CN110852357A (en) * | 2019-10-24 | 2020-02-28 | 开望(杭州)科技有限公司 | Ovulation test paper category detection method |
| CN112410439A (en) * | 2020-12-11 | 2021-02-26 | 华南农业大学 | Application of chi-miR-128-3p as goat follicle maturation miRNA marker |
Non-Patent Citations (4)
| Title |
|---|
| K.MENSE, ET AL.: "The somatotropic axis during the physiological estrus cycle in dairy heifers—Effect on hepatic expression of GHR and SOCS2.", 《J. DAIRY SCI.》 * |
| 余文莉等: "绒山羊超数排卵和胚胎冷冻技术的初步研究", 《中国养羊》 * |
| 吕俊杰等: "阻滞和非阻滞品系小鼠次级卵母细胞基因表达水平的比较", 《解剖学杂志》 * |
| 孙丽君等: "卵巢正常反应不孕症患者体外受精不同长方案促排卵效果的研究", 《中华生殖与避孕杂志》 * |
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