CN113341118A - Method for testing clinical test items - Google Patents
Method for testing clinical test items Download PDFInfo
- Publication number
- CN113341118A CN113341118A CN202110391041.8A CN202110391041A CN113341118A CN 113341118 A CN113341118 A CN 113341118A CN 202110391041 A CN202110391041 A CN 202110391041A CN 113341118 A CN113341118 A CN 113341118A
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- China
- Prior art keywords
- test tube
- filter paper
- body fluid
- centrifuge
- taking
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Abstract
The invention discloses a method for inspecting a clinical inspection item, which belongs to the technical field of medical inspection and comprises the following steps: collecting body fluid, placing the body fluid into a test tube for sealing, marking sampling information on the outer wall of the test tube, and sending the sealed embassy into a laboratory for detection; taking out the test tube by an experimenter, taking down the sealing cover, taking a new test tube for later use, filling filter paper in the new test tube, enabling the filter paper to be tightly attached to the inner wall of the test tube, and pouring body fluid into the new test tube for sealing; placing the test tube filled with the filter paper into a centrifuge for processing, so that the clear liquid floats upwards, the turbid liquid sinks and is attached to the filter paper, and the centrifuge performs centrifugal processing on the body fluid at three rotating speeds; the test tube after centrifugation is taken out, the sealing cover is taken down, the filter paper is quickly taken out of the test tube, and the settled turbid liquid is taken down from the filter paper.
Description
Technical Field
The invention relates to the technical field of medical inspection, in particular to an inspection method of a clinical inspection item.
Background
Medical testing is a science of microbiology, immunology, biochemistry, genetics, hematology, biophysics, cytology, etc., of materials taken from the human body, providing information for the prevention, diagnosis, treatment of human diseases and assessment of human health. Through system learning, we will know how to identify a person's blood type, determine if a person is anemic, if liver function is normal, and so on. The subject requires various photoelectric instruments and chemical reagents to complete experimental analysis, so that better biological and chemical bases are required in the science of the important science.
The clinical examination refers to the results of a series of laboratory tests and items provided by clinical laboratories (which can be clinical laboratories, laboratories or independent laboratories in hospitals) for clinical medicine, and is used for disease diagnosis, wherein the clinical examination refers to the detection of blood, body fluid, secretion, excrement, fallen matter and other specimens of patients by visual observation, physical, chemical, instrument or molecular biological methods, and emphasizes that strict quality management measures are taken for the whole examination process (before, during and after analysis) to ensure the examination quality; thereby providing valuable experimental data for clinic and patients. Clinical examination is a comprehensive application subject of interdiffusion and cross fusion of multiple subjects, and relates to the subjects of chemistry, physics, biology, biochemistry, immunology, microbiology, physiology, pathology, genetics, molecular biology, statistics, multiple clinical medicine and the like.
Need collect the body fluid of being surveyed the person when detecting body fluid among the current clinical examination, then utilize centrifuge to carry out the separation of clear liquid and turbid liquid, generally only utilize centrifuge's high-speed to separate, because the high-speed rotation lacks the settling time of turbid liquid, cause the accuracy that the incomplete influence of clear and turbid liquid separation detected, need can extract turbid liquid after taking out the clear liquid through centrifuge separation turbid liquid, seriously influence detection efficiency.
Disclosure of Invention
The invention aims to provide a method for inspecting clinical inspection items, which aims to solve the problems that in the existing clinical inspection proposed in the background art, the body fluid of an inspected person needs to be collected when the body fluid is inspected, then a centrifugal machine is used for separating clear liquid from turbid liquid, generally the centrifugal machine is only used for separating the clear liquid at high speed, the separation of the clear liquid and the turbid liquid does not completely influence the accuracy of the inspection due to the lack of the settling time of the turbid liquid due to the high-speed rotation, and the clear liquid can be extracted into the turbid liquid only after the separation of the turbid liquid by the centrifugal machine, so that the inspection efficiency is seriously influenced.
In order to achieve the purpose, the invention provides the following technical scheme: a method for testing a clinical test item, comprising the steps of:
s1: collecting body fluid, placing the body fluid into a test tube for sealing, marking sampling information on the outer wall of the test tube, and sending the sealed embassy into a laboratory for detection;
s2: taking out the test tube by an experimenter, taking down the sealing cover, taking a new test tube for later use, filling filter paper in the new test tube, enabling the filter paper to be tightly attached to the inner wall of the test tube, and pouring body fluid into the new test tube for sealing;
s3: placing the test tube filled with the filter paper into a centrifuge for processing, so that the clear liquid floats upwards, the turbid liquid sinks and is attached to the filter paper, and the centrifuge performs centrifugal processing on the body fluid at three rotating speeds;
s4: the test tube after centrifugation is taken out, the sealing cover is taken down, the filter paper is quickly taken out from the test tube, the turbid liquid of the precipitate is taken down from the filter paper and evenly distributed on the glass slide, a plurality of visual fields are observed by a high power lens, and the average value of the visual fields is taken.
Preferably, the sampling information includes basic information of the person to be sampled, a sampling number, and a sampling type.
Preferably, the basic information of the person to be sampled includes the name, sex and age of the person to be sampled.
Preferably, the rotating speed of the centrifuge is 1500r/min, the centrifugation time is 5min, the rotating speed of the centrifuge is reduced to 1000r/min after 5min, the centrifugation time is 10 min, and finally the rotating speed of the centrifuge is reduced to 500r/min, and the centrifugation time is 5 min.
Preferably, the field of view observed by the high power lens is 10 fields of view.
Compared with the prior art, the invention has the beneficial effects that: the invention improves the detection efficiency, enables the body fluid to be evenly precipitated by adjusting the rotating speed of the centrifuge, improves the detection accuracy, puts the test tube with the filter paper into the centrifuge for processing, enables the clear liquid to float upwards, enables the turbid liquid to sink and attach to the filter paper, and the centrifuge carries out centrifugal processing on the body fluid by three rotating speeds, firstly, the rotating speed of the centrifuge is 1500r/min, the centrifugal time is 5min, enables the turbid liquid in the body fluid to be rapidly separated from the clear liquid by high-speed rotation, reduces the rotating speed of the centrifuge to 1000r/min after 5min, reduces the rotating speed on the high-speed basis, facilitates the precipitation of the turbid liquid, the centrifugal time is 10 min, finally reduces the rotating speed of the centrifuge to 500r/min, reduces the centrifugal time to 5min, reduces to the low speed, enables the turbid liquid to be thoroughly separated from the clear liquid in the process of three-level speed reduction, and can effectively enable the turbid liquid to fully attach to the filter paper, the effectual test accuracy that has improved takes out the test tube after the centrifugation, takes off sealed lid, and quick taking out filter paper from the test tube, taking off the turbid liquid of sediment from filter paper, evenly distributed is on the slide glass, observes 10 fields of vision with the high power mirror, gets its average value, directly takes out filter paper and can detect, need not like traditional mode, need clear away the back with the clear solution, takes out turbid liquid again and detects, has improved detection efficiency.
Drawings
FIG. 1 is a block diagram showing the flow of the inspection method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention provides a method for inspecting clinical test items, which improves the testing efficiency, and by adjusting the rotation speed of a centrifuge, the body fluid can be precipitated uniformly, and the testing accuracy is improved, please refer to FIG. 1,
the method for testing the clinical test items comprises the following steps:
s1: collecting body fluid, placing the body fluid into a test tube for sealing, marking the name, sex and age of a person to be sampled on the outer wall of the test tube, and whether the collected sample is saliva or urine, and sending the sealed embassy into a laboratory for detection;
s2: taking out the test tube by an experimenter, taking down the sealing cover, taking a new test tube for later use, filling filter paper in the new test tube, enabling the filter paper to be tightly attached to the inner wall of the test tube, and pouring body fluid into the new test tube for sealing;
s3: placing a test tube filled with filter paper into a centrifuge for processing, so that clear liquid floats upwards, turbid liquid sinks to be attached to the filter paper, centrifuging body fluid by the centrifuge at three rotating speeds, firstly, the rotating speed of the centrifuge is 1500r/min, the centrifuging time is 5min, rapidly separating the turbid liquid from the clear liquid in the body fluid through high-speed rotation, reducing the rotating speed of the centrifuge to 1000r/min after 5min, reducing the rotating speed on the basis of high speed to facilitate the precipitation of the turbid liquid, the centrifuging time is 10 min, finally, reducing the rotating speed of the centrifuge to 500r/min, the centrifuging time is 5min, reducing the rotating speed to low speed, and completely separating the turbid liquid from the clear liquid in the process of three-level speed reduction, so that the turbid liquid can be effectively and fully attached to the filter paper, and the detection accuracy is effectively improved;
s4: the test tube after will centrifuging takes out, takes off sealed lid, and quick taking out filter paper from the in vitro takes off, takes off the turbid liquid of sediment from filter paper, evenly distributed on the slide glass, observes 10 fields of vision with the high power mirror, gets its average value, directly takes out filter paper and can detect, need not like traditional mode, need clear away the back with the clear solution, takes out turbid liquid again and detects, has improved detection efficiency.
While the invention has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the various features of the embodiments disclosed herein may be used in any combination, provided that there is no structural conflict, and the combinations are not exhaustively described in this specification merely for the sake of brevity and conservation of resources. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (5)
1. A method for testing clinical test items, comprising: the method for testing the clinical test items comprises the following steps:
s1: collecting body fluid, placing the body fluid into a test tube for sealing, marking sampling information on the outer wall of the test tube, and sending the sealed embassy into a laboratory for detection;
s2: taking out the test tube by an experimenter, taking down the sealing cover, taking a new test tube for later use, filling filter paper in the new test tube, enabling the filter paper to be tightly attached to the inner wall of the test tube, and pouring body fluid into the new test tube for sealing;
s3: placing the test tube filled with the filter paper into a centrifuge for processing, so that the clear liquid floats upwards, the turbid liquid sinks and is attached to the filter paper, and the centrifuge performs centrifugal processing on the body fluid at three rotating speeds;
s4: the test tube after centrifugation is taken out, the sealing cover is taken down, the filter paper is quickly taken out from the test tube, the turbid liquid of the precipitate is taken down from the filter paper and evenly distributed on the glass slide, a plurality of visual fields are observed by a high power lens, and the average value of the visual fields is taken.
2. The method of claim 1, wherein the clinical test item is selected from the group consisting of: the sampling information includes basic information of a person to be sampled, a sampling number, and a sampling type.
3. The method of claim 2, wherein the clinical test item is selected from the group consisting of: the basic information of the sampled person comprises the name, the sex and the age of the sampled person.
4. The method of claim 1, wherein the clinical test item is selected from the group consisting of: the rotating speed of the centrifuge is 1500r/min, the centrifugation time is 5min, the rotating speed of the centrifuge is reduced to 1000r/min after 5min, the centrifugation time is 10 min, and finally the rotating speed of the centrifuge is reduced to 500r/min, and the centrifugation time is 5 min.
5. The method of claim 1, wherein the clinical test item is selected from the group consisting of: the visual field observed by the high power lens is 10 visual fields.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110391041.8A CN113341118A (en) | 2021-04-12 | 2021-04-12 | Method for testing clinical test items |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110391041.8A CN113341118A (en) | 2021-04-12 | 2021-04-12 | Method for testing clinical test items |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113341118A true CN113341118A (en) | 2021-09-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110391041.8A Pending CN113341118A (en) | 2021-04-12 | 2021-04-12 | Method for testing clinical test items |
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|---|---|
| CN (1) | CN113341118A (en) |
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2021
- 2021-04-12 CN CN202110391041.8A patent/CN113341118A/en active Pending
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