CN113322204A - MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof - Google Patents
MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an MOF-Ni/nano titanium dioxide microbial accelerant and a preparation method thereof, and particularly relates to an MOF-Ni/nano titanium dioxide dispersion liquid, a bacterial liquid and a culture medium. The preparation method comprises the steps of adding the MOF-Ni/nano titanium dioxide dispersion liquid into a sterilized culture medium to obtain a mixed liquid B; and adding the bacterial liquid into the mixed liquid B to obtain the microbial accelerant. The MOF-Ni/nano titanium dioxide microbial accelerant not only can improve the activity of urease so as to improve the yield of calcium carbonate, but also can be used as a carrier to increase nucleation sites in an experiment of microbial calcium carbonate induction, and can accelerate the precipitation of calcium carbonate when the calcium carbonate is induced to precipitate due to the adsorption function of the nano titanium dioxide, so that the mineralization of microorganisms is promoted.
Description
Technical Field
The invention relates to an MOF-Ni/nano titanium dioxide microbial accelerant and a preparation method thereof, belonging to the technical field of dust prevention and suppression of coal mines.
Background
Coal is taken as a non-renewable resource, is one of three major energy sources in the world economy at present, is also one of main channels of energy supply in China, and the production scale and consumption level of the coal in China are ranked first in all countries in the world. Among them, opencast coal mine production is the dominant one in the coal production process. Because coal is not properly controlled in the processes of transportation, storage and mining, a large amount of coal dust is generated in the using process, and the coal dust is also the most important pollutant in the coal mining process. The coal dust is released and mixed with dust on the transportation road, which damages the surrounding environment and harms human health, and is mainly reflected in the following aspects: firstly, coal dust and dust can generate spontaneous combustion explosion when reaching a certain concentration, thereby causing accidents; workers working in coal mines can seriously threaten the physical and psychological health of coal mine workers due to the increase of the respiratory dust, and the probability of pneumoconiosis and other lung diseases can be increased; and thirdly, for equipment, the abrasion degree of the equipment can be increased by the dust, so that the precision is reduced, and further accidents are caused. The current method for spraying the chemical dust suppressant has the defects of high cost, large environmental pollution, difficult degradation and the like, and can cause secondary pollution. Therefore, the key point is to research and develop the microbial dust suppressant which has no secondary pollution, good dust suppression effect and low price and solve the problems of the current chemical dust suppressant. However, the existing microbial dust suppressant has the problems of long mineralization time, low calcium carbonate yield and few nucleation sites in the use process.
Disclosure of Invention
The invention aims at the problems and provides an MOF-Ni/nano titanium dioxide microbial accelerant and a preparation method thereof. Meanwhile, the MOF-Ni/nano titanium dioxide material serving as a carrier can increase nucleation sites to promote the precipitation of calcium carbonate; further provides the MOF-Ni/nano titanium dioxide microbial accelerant which is low in cost, green and environment-friendly and can reduce the mineralization time. The technical scheme of the invention is as follows:
an MOF-Ni/nano titanium dioxide microbial accelerant specifically comprises an MOF-Ni/nano titanium dioxide dispersion liquid, a bacterial liquid and a culture medium.
Preferably, the volume ratio of the MOF-Ni/nano titanium dioxide dispersion liquid to the bacterial liquid to the culture medium is 0.1:75: 7.5.
Further, the preparation method of the MOF-Ni/nano titanium dioxide comprises the following steps:
(1) preparing precursor MOF-Ni: mixing Ni (NO)3)2·6H2Adding O and terephthalic acid into dimethyl amide, stirring until the solid is completely dissolved, adding ethylene glycol, continuously stirring for 20-30 min to obtain a mixed solution A, then transferring the mixed solution A into a reaction kettle, placing the reaction kettle into an oven, heating for 6-12 h at the temperature of 120-130 ℃, and finally sequentially centrifuging, washing, drying and grinding to obtain a precursor MOF-Ni;
ni (NO) in the mixed solution A3)2·6H2The molar concentration of O is 0.018-0.022 mol/L;
the molar concentration of the terephthalic acid in the mixed solution A is 0.007-0.012 mol/L;
the volume ratio of the ethylene glycol to the dimethylformamide is (0.12-0.35) to 1;
(2) preparation of MOF-Ni/nano titanium dioxide: adding deionized water into the precursor MOF-Ni prepared in the step (1) for ultrasonic dispersion for 5-10 min to obtain a precursor MOF-Ni with a concentration of 0.5-0.75 g/L, adding nano titanium dioxide under stirring, stirring for 10-20 min, placing the reaction kettle in an oven, heating for 8-20 h at 18-200 ℃, and then sequentially carrying out de-hilling washing and drying to obtain powder; finally obtaining MOF-Ni/nano titanium dioxide; and carrying out ultrasonic dispersion on the MOF-Ni/nano titanium dioxide and 1% of starch to prepare an MOF-Ni/nano titanium dioxide dispersion liquid.
Washing in the step (2) is carried out by sequentially adopting deionized water and absolute ethyl alcohol; the volume ratio of the deionized water to the absolute ethyl alcohol is (0.05-0.08): 1;
the concentration of the MOF-Ni/nano titanium dioxide dispersion liquid is 10-20 g/L.
Further, the nano titanium dioxide is at least one selected from anatase hydrophilic nano titanium dioxide, anatase lipophilic nano titanium dioxide and anatase hydrophilic lipophilic nano titanium dioxide, and the diameter of the nano titanium dioxide is 5-25 nm.
The bacteria in the bacterial liquid are selected from one or more urease-producing bacteria such as bacillus pasteurii, bacillus cereus, bacillus megaterium, bacillus sphaericus and the like, and the concentration of the bacteria in the bacterial liquid is 1 multiplied by 107～1×1010Each colony per ml.
The raw materials in the culture medium are lipopeptide biosurfactant or rhamnolipid and K2HPO4、NiCl2Or Ni (NO)3) Peptone, sodium chloride, glucose, urea;
further, the preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
adding the MOF-Ni/nano titanium dioxide dispersion liquid into a sterilized culture medium to obtain a mixed liquid B; and adding the bacterial liquid into the mixed liquid B to obtain the microbial accelerant.
A microbial accelerant comprises the following raw materials in parts by weight in a culture medium: 1-10 parts of MOF-Ni/nano titanium dioxide dispersion liquid, 100-150 parts of lipopeptide biosurfactant or rhamnolipid, and K2HPO4500-1000 parts of CO (NH)2)22000-3000 parts of NiCl2Or Ni (NO)3) 1-5 parts of peptone, 1000-2000 parts of sodium chloride and 1000-2000 parts of glucose.
The MOF-Ni/nano titanium dioxide microbial accelerant mainly utilizes MOF-Ni/nano titanium dioxide to provide nickel ions for promoting urease activity and slowly release nano titanium dioxide. The nanometer titanium dioxide can generate free radicals with high catalytic activity under the action of sunlight and ultraviolet rays, and can generate strong photooxidation and reduction capability. The MOF-Ni/nano titanium dioxide can also provide nucleation sites to promote the production of calcium carbonate.
By adding lipopeptide biosurfactant or rhamnolipid, stronger phospholipid substances can be used for replacing strong hydrophilic substances on the surfaces of bacteria, so that the lipophilicity of the surfaces of the microorganisms is enhanced, the size of a contact angle is reduced, the permeability is enhanced, and the adsorbability is enhanced. In addition, lipopeptide biosurfactants or rhamnolipids can also increase adhesion, allowing a large number of microorganisms to adhere to each other to form aggregates.
By adding NiCl2Or Ni (NO)3) The reagent, namely nickel ions are added in a liquid phase environment, and the added nickel ions are not only trace elements for the growth of microorganisms, but also one of inorganic salts.
By addition of CO (NH)2)2The reagent is added with decomposed substances of urease-producing bacteria for producing urease in a liquid phase environment, and can be decomposed to produce ammonium ions and carbonate ions.
Finally, K2HPO4The inorganic salt component provides a material basis for the normal degradation of the microorganism growth. Namely, the MOF-Ni/nano titanium dioxide microbial accelerant provided by the invention can effectively provide basic guarantee for the microbial induction of calcium carbonate precipitation, improve the activity of urease, increase the precipitation of calcium carbonate, solve the problems of hydrophobicity and the like of microorganisms, and simultaneously realize the characteristics of environmental protection, no pollution and low cost.
The usage method of the MOF-Ni/nano titanium dioxide microbial accelerant is as follows:
(3) spraying MOF-Ni/nano titanium dioxide microbial accelerant to the coal dust;
(4) spraying a mineralized substrate on the basis of the step (1).
The mineralized substrate is selected from calcium chloride and calcium lactateAt least one of calcium nitrate, calcium formate or calcium acetate; the mineralized substrate and CO (NH) in the culture medium2)2The concentration ratio of (1) to (1).
Compared with the prior art, the invention has the following advantages:
(1) the MOF-Ni/nano titanium dioxide dispersion liquid which is nontoxic to microorganisms is added into the MOF-Ni/nano titanium dioxide microbial accelerant, so that the penetration of a bacterial liquid and a mineralized substrate in the using process is increased, and the thickness of a coal dust consolidation layer is increased;
(2) the urease-producing bacteria can produce urease in the culture process, the urease can decompose urea to produce calcium carbonate ions, and the produced calcium carbonate ions can be combined with calcium ions in the mineralized liquid to form calcium carbonate in the use process, so that the environment-friendly calcium carbonate mineralized liquid is green and pollution-free;
(3) the surface of the pulverized coal is solidified by utilizing the bacterial mineralization, so that the method has the advantages of no secondary pollution, low cost, good effect, simplicity and easiness in operation, has a good application prospect in open-pit coal mines and is worthy of large-scale popularization;
(4) compared with chemical dust depressors and common biological dust depressors, the MOF-Ni/nano titanium dioxide microbial accelerant has the characteristics of simple preparation, convenient construction, greenness and no pollution;
(5) compared with the common microbial dust suppressant, the MOF-Ni/nano titanium dioxide microbial accelerant has MOF-Ni/nano titanium dioxide dispersion liquid, and the nano titanium dioxide has photocatalytic performance and shortens mineralization time.
Drawings
FIG. 1 is a growth curve of bacteria under different concentrations of MOF-Ni/nano titanium dioxide microbial accelerant;
FIG. 2 shows the amount of calcium carbonate produced by MOF-Ni/nano titanium dioxide microbial accelerator with different concentrations;
FIG. 3 MOF-Ni/nano-titania microbial accelerant penetration depth tests at different concentrations;
FIG. 4 is an electron micrograph of calcium carbonate production by MOF-Ni/nano titanium dioxide microbial promoters at different concentrations.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Example 1: MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof
(1) Preparation of precursor MOF-Ni: 0.018mol/L of Ni (NO)3)2·6H2Adding O and 0.007mol/L terephthalic acid into dimethyl amide, stirring until the solid is completely dissolved, adding ethylene glycol, continuously stirring for 20-30 min to obtain a mixed solution A, then moving the mixed solution A into a reaction kettle, placing the reaction kettle into an oven, heating for 6h at the temperature of 120 ℃, and finally sequentially centrifuging, washing and drying to obtain a precursor MOF-Ni;
(2) ultrasonically dispersing a precursor MOF-Ni in deionized water for 5min, dropwise adding nano titanium dioxide under the stirring condition, stirring for 10min, placing a reaction kettle in an oven, heating for 8h at 180 ℃, and then sequentially carrying out de-hilling washing and drying to obtain powder; finally obtaining MOF-Ni/nano titanium dioxide; and carrying out ultrasonic dispersion on the MOF-Ni/nano titanium dioxide and 1% of starch to prepare an MOF-Ni/nano titanium dioxide dispersion liquid.
The preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
the preparation concentration is 0.23g/L rhamnolipid, 0.2g/L K2HPO4，0.1g/L NiCl2900ml of a mixed solution of 10g/L peptone and 10g/L sodium chloride was adjusted to pH 7, and sterilized by autoclaving at 121 ℃ for 20 min. 100ml of mixed solution of 10g/L glucose and 20g/L urea is sterilized by a filter head, and the sterilized mixed solution of glucose and urea is added after peptone and sodium chloride solution are cooled. The mixture is divided into 18 conical flasks, and 1% of the bacterial liquid is added into each conical flask. Parallel experiments were designed, one set of three bottles each, one set being a control experiment. Adding MOF-Ni/nano titanium dioxideThe concentration gradient of the dispersion is 0.03g/L, 0.06g/L, 0.09g/L and 0.12 g/L. The flask was placed in a constant temperature shaking incubator at 150rpm at 25 ℃ for 24 hours. And 3ml of the MOF-Ni/nano titanium dioxide microbial accelerant in the conical flask is taken in a sterilized ultra-clean workbench at intervals within 24h, and the absorbance at the wavelength of 600nm is measured by an ultraviolet spectrophotometer to reflect the growth curve of the bacteria. The measured results are shown in figure 1, and figure 1 is a growth curve of bacteria under different concentrations of MOF-Ni/nano titanium dioxide microbial promoters.
Example 2: MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof
The preparation method of the MOF-Ni/nano titanium dioxide is the same as that of the example 1.
The preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
the preparation concentration is 0.23g/L rhamnolipid, 0.2g/L K2HPO4，0.1g/L NiCl2900ml of a mixed solution of 10g/L peptone and 10g/L sodium chloride was adjusted to pH 7, and sterilized by autoclaving at 121 ℃ for 20 min. 100ml of mixed solution of 10g/L glucose and 20g/L urea is sterilized by a filter head, and the sterilized mixed solution of glucose and urea is added after peptone and sodium chloride solution are cooled. The mixture is divided into 18 conical flasks, and 1% of the bacterial liquid is added into each conical flask. Parallel experiments were designed, one set of three bottles each, one set being a control experiment. The concentration gradient of the added MOF-Ni/nano titanium dioxide dispersion liquid is 0.03g/L, 0.06g/L, 0.09g/L and 0.12 g/L. The flask was placed in a constant temperature shaking incubator at 150rpm at 25 ℃ for 24 hours. Adding mineralized substrate and culturing for 7 d.
And (3) testing:
drying the conical flask in a constant temperature incubator at 100 ℃, weighing the flask and the precipitate to obtain W1(ii) a Washing with hydrochloric acid to remove calcium carbonate precipitate, oven drying, and weighing to obtain W2(ii) a The weight of calcium carbonate is W2-W1。
TABLE 1 calcium carbonate production by MOF-Ni/nano-titanium dioxide microbial accelerator with different concentrations
The amount of calcium carbonate produced by MOF-Ni/nano titanium dioxide microbial accelerator with different concentrations is shown in figure 2;
as shown in figure 4, the electron microscope images of calcium carbonate production by MOF-Ni/nano titanium dioxide microbial accelerant with different concentrations;
as can be seen from Table 1, the addition of different concentrations of MOF-Ni/nano titanium dioxide microbial promoters resulted in a decrease after an increase in the calcium carbonate content. When the concentration of the MOF-Ni/nano titanium dioxide microbial accelerant is 0.06, the content of calcium carbonate generated by adding the accelerant is increased by 37.1 percent, because the MOF-Ni/nano titanium dioxide can release the nano titanium dioxide to improve the activity of urease; when the concentration of the MOF-Ni/nano titanium dioxide is 0.09, the reason that the yield of the calcium carbonate is reduced is that the nano titanium dioxide has a certain bactericidal effect and can influence the growth of bacteria; when the concentration of the MOF-Ni/nano titanium dioxide is 0.12, the yield of the calcium carbonate is increased because the nano titanium dioxide can be used as a nucleation site, and the yield of the calcium carbonate is increased. In conclusion, the MOF-Ni/nano titanium dioxide microbial accelerant can promote the yield of calcium carbonate.
Example 3: MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof
The preparation method of the MOF-Ni/nano titanium dioxide is the same as that of the example 1.
The preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
the preparation concentration is 0.23g/L rhamnolipid, 0.2g/L K2HPO4，0.1g/L NiCl2900ml of a mixed solution of 10g/L peptone and 10g/L sodium chloride was adjusted to pH 7, and sterilized by autoclaving at 121 ℃ for 20 min. 100ml of mixed solution of 10g/L glucose and 20g/L urea is sterilized by a filter head, and the sterilized mixed solution of glucose and urea is added after peptone and sodium chloride solution are cooled. The mixture is divided into 18 conical flasks, and 1% of the bacterial liquid is added into each conical flask. Parallel experiments were designed, one set of three bottles each, one set being a control experiment. Adding MOF-Ni/nano titanium dioxideThe concentration was 0.06 g/L. The flask was placed in a constant temperature shaking incubator at 150rpm at 25 ℃ for 24 hours.
And (3) testing:
30g of coal powder and 120-mesh coal powder are weighed by a culture dish, then the prepared solutions are respectively placed in a watering can, and the mixed solution of the MOF-Ni/nano titanium dioxide microbial accelerator and the mineralized substrate are sequentially arranged. The volume ratio is 10: 1.
And finally, placing the coal powder sprayed with the MOF-Ni/nano titanium dioxide microbial accelerant at room temperature for natural air drying, spraying the MOF-Ni/nano titanium dioxide microbial accelerant again according to the two modes after 3d, repeating the operation for 4 times, and placing the treated coal powder at room temperature for natural air drying for 15 d. (treatment mode 1)
As a control, the microbial promoter without MOF-Ni/nano titanium dioxide was treatment mode 2.
After the treatment, the treated coal dust was subjected to a weathering test at a wind speed of 10m/s, the results of which are shown in Table 2.
TABLE 2 wind erosion rates of different treated coal powders at different times
As can be seen from Table 2, the treatment mode with the addition of the MOF-Ni/nano titanium dioxide microbial accelerant has a wind erosion rate of 75.5% less than that of the treatment mode without the addition of the MOF-Ni/nano titanium dioxide microbial accelerant in the 8h wind erosion process. It can be seen from table 2 that the coal dust loss effect of the treatment mode 1 is far better than that of the treatment mode 2, because the coal dust of the treatment mode 1 is added with the microbial accelerant of the MOF-Ni/nano titanium dioxide, more mineralized products can be produced, the cementation of the coal dust can be realized, and a better dust suppression effect can be achieved.
Example 4: MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof
The preparation method of the MOF-Ni/nano titanium dioxide is the same as that of the example 1.
The preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
the preparation concentration is 0.23g/L rhamnolipid, 0.2g/L K2HPO4，0.1g/L NiCl2900ml of a mixed solution of 10g/L peptone and 10g/L sodium chloride was adjusted to pH 7, and sterilized by autoclaving at 121 ℃ for 20 min. 100ml of mixed solution of 10g/L glucose and 20g/L urea is sterilized by a filter head, and the sterilized mixed solution of glucose and urea is added after peptone and sodium chloride solution are cooled. The mixture is divided into 18 conical flasks, and 1% of the bacterial liquid is added into each conical flask. Parallel experiments were designed, one set of three bottles each, one set being a control experiment. The concentration of the added MOF-Ni/nano titanium dioxide is 0.06 g/L. The flask was placed in a constant temperature shaking incubator at 150rpm at 25 ℃ for 24 hours.
And (3) testing:
30g of coal powder and 120-mesh coal powder are weighed by a culture dish, then the prepared solutions are respectively placed in a watering can, and the mixed solution of the MOF-Ni/nano titanium dioxide microbial accelerator and the mineralized substrate are sequentially arranged. The volume ratio is 10: 1.
And finally, placing the coal powder sprayed with the MOF-Ni/nano titanium dioxide microbial accelerant at room temperature for natural air drying, spraying the MOF-Ni/nano titanium dioxide microbial accelerant again according to the two modes after 3d, repeating the operation for 4 times, and placing the treated coal powder at room temperature for natural air drying for 15 d. (treatment mode 1)
As a control, the microbial promoter without MOF-Ni/nano titanium dioxide was treatment mode 2.
After the treatment, the treated coal powder is subjected to an evaporation rate resistance experiment, and the balance measures that the total weight of the sprayed glass vessel is V1Then putting the mixture into a constant temperature incubator at 35 ℃ for 8 hours, taking out the mixture and weighing the mixture to obtain a total weight V2And measuring the water evaporation capacity of the MOF-Ni/nano titanium dioxide microbial accelerant per unit area and unit time for each three parallel samples.
η＝V1-V2/ST
S, area of the glass vessel;
t-time
As a control, the microbial promoter without MOF-Ni/nano titania was treated as treatment 2, and the results are shown in table 3.
TABLE 3 different treatment coal dust evaporation rates
As can be seen from Table 3, the evaporation resistance of the treatment mode with the MOF-Ni/nano titanium dioxide microbial accelerant is improved by 1 time compared with the evaporation resistance of the treatment mode without the MOF-Ni/nano titanium dioxide microbial accelerant, and a better evaporation resistance effect can be achieved.
Example 5: MOF-Ni/nano titanium dioxide microbial accelerant and preparation method thereof
The preparation method of the MOF-Ni/nano titanium dioxide is the same as that of the example 1.
The preparation method of the MOF-Ni/nano titanium dioxide microbial accelerant comprises the following steps:
preparing 900ml of APG solution with the concentration of 0.23g/L and mixed solution of 10g/L peptone and 10g/L sodium chloride, adjusting the pH value to 7, and sterilizing for 20min at 121 ℃ in an autoclave by using high-pressure steam. 100ml of mixed solution of 10g/L glucose and 20g/L urea is sterilized by a filter head, and the sterilized mixed solution of glucose and urea is added after peptone and sodium chloride solution are cooled. The mixture is divided into 18 conical flasks, and 1% of the bacterial liquid is added into each conical flask. Parallel experiments were designed, one set of three bottles each, one set being a control experiment. The concentration gradient of the added MOF-Ni/nano titanium dioxide is 0.03g/L, 0.06g/L, 0.09g/L and 0.12 g/L. The flask was placed in a constant temperature shaking incubator at 150rpm at 25 ℃ for 24 hours.
After the treatment is finished, performing a permeability rate experiment on the treated coal dust, filling a certain amount of coal dust into a test tube with phi multiplied by h of 20 multiplied by 200mm, compacting the coal dust by using a glass rod to ensure that the height of the coal dust filled in the test tube is 15cm, and then fixing the coal dust on a test tube rack; finally, 2ml of dust suppression solution is slowly added, the dust suppression solution is slowly dropped into a glass tube filled with coal powder as shown in figure 3, the penetration depth within 20min is measured by a tape measure, and the result is shown in table 4; FIG. 3 is a MOF-Ni/nano titanium dioxide microbial accelerant penetration depth test at different concentrations.
TABLE 4 penetration depth of coal dust
As can be seen from Table 4, the effect of permeation with the addition of the MOF-Ni/nano titanium dioxide microbial enhancer is better than that without the addition of the MOF-Ni/nano titanium dioxide microbial enhancer.
Claims (10)
1. A MOF-Ni/nano titanium dioxide microbial accelerant is characterized by comprising an MOF-Ni/nano titanium dioxide dispersion liquid, a bacterial liquid and a culture medium.
2. The microbial growth promoter of claim 1, wherein the volume ratio of the MOF-Ni/nano titanium dioxide dispersion to the bacterial liquid to the culture medium is 0.1:75: 7.5.
3. The microbial growth promoter according to claim 1 or 2, wherein the MOF-Ni/nano titanium dioxide is prepared by the following steps:
(1) preparing precursor MOF-Ni: mixing Ni (NO)3)2·6H2Adding O and terephthalic acid into dimethyl amide, stirring until the solid is completely dissolved, adding ethylene glycol, continuously stirring for 20-30 min to obtain a mixed solution A, then transferring the mixed solution A into a reaction kettle, placing the reaction kettle into an oven, heating for 6-12 h at the temperature of 120-130 ℃, and finally sequentially centrifuging, washing, drying and grinding to obtain a precursor MOF-Ni;
ni (NO) in the mixed solution A3)2·6H2The molar concentration of O is 0.018-0.022 mol/L;
the molar concentration of the terephthalic acid in the mixed solution A is 0.007-0.012 mol/L;
the volume ratio of the ethylene glycol to the dimethylformamide is (0.12-0.35) to 1;
(2) preparation of MOF-Ni/nano titanium dioxide: adding deionized water into the precursor MOF-Ni prepared in the step (1) for ultrasonic dispersion for 5-10 min to obtain a precursor MOF-Ni with a concentration of 0.5-0.75 g/L, adding nano titanium dioxide under stirring, stirring for 10-20 min, placing the reaction kettle in an oven, heating for 8-20 h at 18-200 ℃, and then sequentially carrying out de-hilling washing and drying to obtain powder; finally obtaining MOF-Ni/nano titanium dioxide; carrying out ultrasonic dispersion on the MOF-Ni/nano titanium dioxide by adding 1% of starch to prepare an MOF-Ni/nano titanium dioxide dispersion liquid; the washing is sequentially carried out by using deionized water and absolute ethyl alcohol, and the volume ratio of the deionized water to the absolute ethyl alcohol is (0.05-0.08): 1.
4. The microbial accelerant as claimed in claim 3, wherein the concentration of MOF-Ni/nano titanium dioxide in the MOF-Ni/nano titanium dioxide dispersion liquid is 10-20 g/L.
5. The microbe accelerator as claimed in claim 1, wherein the nano titanium dioxide is at least one selected from anatase hydrophilic nano titanium dioxide, anatase lipophilic nano titanium dioxide, and anatase hydrophilic lipophilic nano titanium dioxide, and the diameter of the nano titanium dioxide is 5-25 nm.
6. The microbe accelerator according to claim 1, wherein the bacteria in the bacterial liquid are selected from one or more urease-producing bacteria such as Bacillus pasteurii, Bacillus cereus, Bacillus megaterium, Bacillus sphaericus, etc., and the concentration of the bacteria in the bacterial liquid is 1X 107～1×1010Each colony per ml.
7. The microbe promoter as claimed in claim 1, wherein the raw material in the culture medium is lipopeptide biosurfactant or rhamnolipid, K2HPO4、NiCl2Or Ni (NO)3) Peptone, sodium chloride, glucose, urea;
8. the microbial growth promoter according to claim 7, wherein the culture medium isComprises the following raw materials in parts by weight: 1-10 parts of MOF-Ni/nano titanium dioxide dispersion liquid, 100-150 parts of lipopeptide biosurfactant or rhamnolipid, and K2HPO4500-1000 parts of CO (NH)2)22000-3000 parts of NiCl2Or Ni (NO)3) 1-5 parts of peptone, 1000-2000 parts of sodium chloride and 1000-2000 parts of glucose.
9. A process for producing a microbial growth promoter as claimed in any one of claims 1 to 8, which comprises the steps of:
adding the MOF-Ni/nano titanium dioxide dispersion liquid into a sterilized culture medium to obtain a mixed liquid B; and adding the bacterial liquid into the mixed liquid B to obtain the microbial accelerant.
10. The use of the microbial growth promoting agent according to any one of claims 1 to 8, comprising the steps of:
(1) spraying MOF-Ni/nano titanium dioxide microbial accelerant to the coal dust;
(2) spraying a mineralized substrate on the basis of the step (1);
the mineralization substrate is selected from at least one of calcium chloride, calcium lactate, calcium nitrate, calcium formate or calcium acetate; the mineralized substrate and CO (NH) in the culture medium2)2The concentration ratio of (1) to (1).
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