CN113322164A - Frozen soil microorganism sampling device and method - Google Patents
Frozen soil microorganism sampling device and method Download PDFInfo
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- CN113322164A CN113322164A CN202110734923.XA CN202110734923A CN113322164A CN 113322164 A CN113322164 A CN 113322164A CN 202110734923 A CN202110734923 A CN 202110734923A CN 113322164 A CN113322164 A CN 113322164A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- G01N1/02—Devices for withdrawing samples
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Abstract
The invention discloses a frozen soil microorganism sampling device and a frozen soil microorganism sampling method, and the frozen soil microorganism sampling device comprises a soil sample collecting device and a microorganism extracting device, wherein the soil sample collecting device conveys collected frozen soil to the microorganism extracting device through a soil sample transferring mechanism for microorganism extraction, the soil sample collecting device comprises a sampling driving mechanism and a supplying mechanism, the supplying mechanism stores a plurality of sampling tubes, and the supplying mechanism supplies the plurality of sampling tubes to the sampling driving mechanism one by one. Through supply mechanism and sample actuating mechanism's cooperation come to realize carrying out a lot of samplings to the frozen soil with a plurality of sampling tubes to carry out the extraction that microorganism was carried out to microorganism extraction element through the sampling tube that soil sample shift mechanism will be equipped with the soil sample, not only realized the integration that soil sample collection and microorganism extracted, and realized the automation to frozen soil independent sampling many times, avoided the manual work to carry out the big drawback of intensity of labour of many times sample existence to the frozen soil.
Description
Technical Field
The invention relates to the technical field of soil microorganism sampling, in particular to a frozen soil microorganism sampling device and method.
Background
The microorganisms include: bacteria, viruses, fungi, and some small protists, microscopic algae, etc., which are small and closely related to humans. The food can be widely applied to various fields such as food, medicine, industry and agriculture, environmental protection, sports and the like.
At present, the research on microorganisms often needs to extract microorganism samples from nature for research, the microorganism research work in the prior art includes the extraction research on microorganisms in soil, soil sampling is often performed manually in places where biological activities such as certain flower beds and lawns are active, but the requirement for sampling microorganisms in frozen soil also exists, the soil of frozen soil is hard and hard at normal temperature, soil samples in different areas and different depths are often required to be collected, the physical consumption of operators is large, the manpower is limited, the efficiency of soil sample collection and the range of soil sample depths capable of being collected are affected, and therefore a device capable of replacing manual sampling from frozen soil is urgently needed.
Disclosure of Invention
The invention aims to provide a frozen soil microorganism sampling device and a frozen soil microorganism sampling method, which aim to solve the technical problems of low efficiency and limited sampling depth of manual sampling of frozen soil in the prior art.
In order to solve the technical problems, the invention specifically provides the following technical scheme:
a frozen soil microorganism sampling device comprises a soil sample collecting device and a microorganism extracting device, wherein the soil sample collecting device conveys collected frozen soil to the microorganism extracting device through a soil sample transferring mechanism to extract microorganisms;
soil sample collection system includes sampling actuating mechanism and supply mechanism, the supply mechanism storage has a plurality of sampling tubes, just supply mechanism will be a plurality of the sampling tube one by one to the sampling actuating mechanism supply, sampling actuating mechanism with the sampling tube that supply mechanism carried is assembled, just sampling actuating mechanism is to rather than the equipment the sampling tube carries out reciprocating drive, so that the sampling tube inserts and takes a sample and extract from the soil layer in the soil layer, soil sample transfer mechanism will the sampling tube that is equipped with soil sample that extracts from the soil layer that sampling actuating mechanism breaks away from to microorganism extraction element carry.
The sampling driving mechanism comprises a telescopic driving cylinder and a cylindrical metal sheath arranged on the telescopic driving cylinder, wherein a filling cavity for filling the sampling tube is formed in the cylindrical metal sheath, the top end of the cylindrical metal sheath is connected with the telescopic driving cylinder, the filling cavity penetrates through the bottom of the cylindrical metal sheath to form a sample inlet for soil samples to enter the sampling tube, an inlet and outlet pipe orifice communicated with the filling cavity for filling the sampling tube is formed in the side wall of the cylindrical metal sheath, and the width of the inlet and outlet pipe orifice is not less than the outer diameter of the sampling tube;
the intracavity is installed and is used for the sampling tube is fixed a position and will be equipped with the soil sample the sampling tube by the business turn over mouth of pipe to the location and the propelling movement subassembly that soil sample transfer mechanism carried.
The tubular metal sheath comprises a pipe orifice protection section, an assembly section and a tail section which are sequentially connected and communicated through the filling cavity, wherein the tail section is connected with the telescopic driving cylinder, the inlet and outlet pipe orifices are arranged on the assembly section, a pipe orifice protection ring for supporting and protecting the pipe orifice of the sampling pipe is arranged on the inner wall of the pipe orifice protection section provided with the sample inlet, and the inner diameter and the outer diameter of the pipe orifice protection ring are not smaller than the inner diameter and the outer diameter of the sampling pipe.
The positioning and pushing assembly comprises an electric push rod, an end positioning block and a translation pushing plate, the translation pushing plate is installed in the filling cavity, the end positioning block is installed at one end, facing the tail section, of the translation pushing plate, the electric push rod is installed on the tail section and connected with the back, opposite to the inlet and outlet pipe orifice, of the translation pushing plate, an electromagnet is installed at the bottom, facing the assembling section, of the end positioning block, and the sampling pipe in the filling cavity is pushed by the electromagnet to abut against the pipe orifice protection ring.
According to a preferable scheme of the invention, the supply mechanism comprises a flat channel with an opening at one side and a pushing cylinder arranged in the other side of the flat channel, and a yielding gap for the soil sample transfer mechanism to transfer the sampling tube is arranged between the opening side of the flat channel and the cylindrical metal sheath;
soil sample shifts mechanism including being used for empty the sampling tube to the transition that the filling chamber shifted changes over to the external member and is used for receiving translation pushing plate releases the external member is rolled over to the transition of sampling tube, the transition change over to the external member with the transition is rolled over the external member and is connected and alternate movement extremely through the same guide dish in the clearance of stepping down, guide dish coaxial arrangement has one end to be located the guide shaft under the clearance of stepping down, the other end transmission of guide shaft is connected with installs servo motor on the outer wall of flat passageway, just it is right to install on the outer wall of flat passageway the support base that the guide shaft supported, run through on the support base seted up with guide shaft normal running fit's shaft hole.
The transition turning-in kit comprises a turning-in platform and an inverted U-shaped transition turning-in channel, wherein the turning-in platform is installed on the side wall of the guide disc, one end of an opening of the transition turning-in channel is installed on the front face of the turning-in platform, which is used for supporting the sampling tube to be transferred into the filling cavity, an input through hole for guiding and limiting the sampling tube is formed between the opening of the transition turning-in channel and the front face of the turning-in platform, and the sampling tube output by the flat channel sequentially passes through the input through hole and the inlet and outlet pipe orifice to enter the filling cavity.
The transition roll-out kit comprises a roll-out platform and a groove-shaped output arm, wherein one end of the groove-shaped output arm is mounted on the roll-out platform, and an output groove for receiving the sampling tube pushed out by the translation push plate is formed in one side of the groove-shaped output arm facing the inlet and outlet tube orifice.
The soil sample collection device, the soil sample transfer mechanism and the microorganism extraction device are arranged in the shell, and the bottom of the shell is provided with a mud scraping hole for the cylindrical metal sheath to pass through;
the microorganism extraction device is positioned behind the cylindrical metal sheath relative to the inlet and outlet pipe orifice, one side of the shell is provided with a soil sample transfer outlet for exposing the groove-shaped output arm in a penetrating way, the shell is provided with a sampling pipe interface in a penetrating way, and the sampling pipe in the output groove of the groove-shaped output arm is butted to the sampling butt joint port of the microorganism extraction device through the sampling pipe interface under the driving of an external power source.
In order to solve the above technical problems, the present invention further provides the following technical solutions:
a sampling method of a frozen soil microorganism sampling device comprises the following steps:
s100, determining the positions and sampling depths of a plurality of sampling points in a frozen soil sampling area;
s200, starting a supply mechanism, wherein the supply mechanism conveys a plurality of sampling tubes stored in the supply mechanism one by one to a cylindrical metal sheath driven by a telescopic driving cylinder;
s300, aligning a sample inlet at the bottom of the cylindrical metal sheath with a sampling point, and setting the stroke of the telescopic driving cylinder according to the sampling depth corresponding to the sampling point;
s400, the telescopic driving cylinder drives the cylindrical metal sheath to be inserted into a soil layer with a corresponding depth of frozen soil according to a set stroke, so that soil below a corresponding sampling point enters the sampling tube from a sample inlet at the bottom of the cylindrical metal sheath, and extruded soil in the sampling tube is retained in the sampling tube in a tensioning mode;
s500, the telescopic driving cylinder drives the cylindrical metal sheath to reset so as to expose the sampling tube with the pattern inside;
s600, the sampling tube filled with the soil sample in the cylindrical metal sheath is transferred to a microorganism extraction device by a soil sample transfer mechanism;
s700, taking out soil in the sampling pipe of the soil sample transfer mechanism by the microorganism extraction device and extracting to finish sampling of frozen soil microorganisms;
and S800, repeating S100-S700 until all the sampling points and the soil samples at the sampling depth are completely collected.
As a preferable mode of the present invention, the soil sample transfer mechanism manually assists in docking the sampling tube removed from the cylindrical metal sheath to a sampling docking port of the microorganism extraction device.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the frozen soil is sampled for multiple times by the plurality of sampling pipes through the matching of the supply mechanism and the sampling driving mechanism, and the sampling pipes filled with the soil samples are conveyed to the microorganism extraction device through the soil sample transfer mechanism to extract the microorganisms, so that the integration of soil sample collection and microorganism extraction is realized, the automation of multiple independent sampling of the frozen soil is realized, and the defect of high labor intensity caused by manual multiple sampling of the frozen soil is avoided.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
FIG. 1 is a schematic overall structure diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a positioning and pushing assembly according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a cylindrical metal sheath according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of a soil sample transfer mechanism according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a transition roll-out kit according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a housing according to an embodiment of the invention.
The reference numerals in the drawings denote the following, respectively:
1-a soil sample collection device; 2-a microorganism extraction device; 3-a soil sample transfer mechanism; 4-sampling tube; 5-filling the cavity; 6-sample inlet; 7-inlet and outlet pipe orifice; 8-positioning and pushing components; 9-a pipe orifice protection ring; 10-abdication clearance; 11-input vias; 12-an output chute; 13-a housing;
101-a sampling drive mechanism; 102-a supply mechanism;
1011-telescopic driving cylinder; 1012-cylindrical metal sheath;
1012 a-nozzle protecting section; 1012 b-Assembly section; 1012 c-end section;
1021-a flat channel; 1022-a push cylinder;
301-transition into the kit; 302-transition out of the kit; 303-a guide disc; 304-a guide shaft; 305-a servo motor; 306-a support base;
3011-transfer to platform; 3012-transiting into the tunnel;
3021-rolling out the platform; 3022-slot type output arm;
801-electric push rod; 802-end positioning block; 803-translating the pusher plate; 804-an electromagnet;
1301-scraping mud holes; 1302-a soil sample transfer outlet; 1303-sampling tube interface.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 6, the invention provides a frozen soil microorganism sampling device, which comprises a soil sample collecting device 1 and a microorganism extracting device 2, wherein the soil sample collecting device 1 conveys collected frozen soil to the microorganism extracting device 2 through a soil sample transferring mechanism 3 for microorganism extraction;
the soil sample collection device 1 comprises a sampling driving mechanism 101 and a supply mechanism 102, wherein the supply mechanism 102 stores a plurality of sampling tubes 4, the supply mechanism 102 supplies the plurality of sampling tubes 4 to the sampling driving mechanism 101 one by one, the sampling driving mechanism 101 is assembled with the sampling tubes 4 conveyed by the supply mechanism 102, the sampling driving mechanism 101 drives the sampling tubes 4 assembled with the sampling driving mechanism to reciprocate, so that the sampling tubes 4 are inserted into soil layers for sampling and pulled out of the soil layers, and the soil sample transfer mechanism 3 conveys the sampling tubes 4 which are pulled out of the soil layers and are separated from the sampling driving mechanism 101 and are filled with soil samples to the microorganism extraction device 2.
The supply mechanism 102 supplies sampling tubes 4 for containing soil samples one by one to the sampling driving mechanism 101, the sampling driving mechanism 101 inserts the empty sampling tubes 4 into the frozen soil, when the sampling tubes 4 are inserted into the frozen soil, the tube walls of the sampling tubes 4 extrude the soil inside and outside the sampling tubes 4, when the sampling tubes 4 are lifted from the frozen soil by the sampling driving mechanism 101, the soil samples collected in the sampling tubes 4 are kept in the sampling tubes 4 under the tensioning action of the soil pressure, so that the sampling tubes 4 collect the frozen soil, and then the sampling tubes 4 containing the soil samples are transferred to the microorganism extraction device 2 through the soil sample transfer mechanism 3, and the microorganism extraction device 2 sequentially takes out, pretreats and extracts the soil samples in the sampling tubes 4.
According to the invention, the frozen soil is sampled for a plurality of times by the plurality of sampling pipes 4 through the matching of the supply mechanism 102 and the sampling driving mechanism 101, and the sampling pipes 4 filled with the soil samples are conveyed to the microorganism extraction device 2 through the soil sample transfer mechanism 3 for microorganism extraction, so that not only is the integration of soil sample collection and microorganism extraction realized, but also the automation of multiple independent sampling of the frozen soil is realized, and the defect of high labor intensity caused by the manual multiple sampling of the frozen soil is avoided.
Wherein, the sampling driving mechanism 101 includes the flexible actuating cylinder 1011 of driving and installs the tube-shape metal sheath 1012 on the flexible actuating cylinder 1011 of driving, the chamber 5 that fills that supplies the sampling tube 4 to pack has been seted up in the tube-shape metal sheath 1012, the top and the flexible actuating cylinder 1011 of driving of tube-shape metal sheath 1012 are connected, fill the chamber 5 and run through the bottom of tube-shape metal sheath 1012 in order to form the introduction port 6 that supplies the soil sample to get into the sampling tube 4, set up the business turn over mouth of pipe 7 that supplies the sampling tube 4 that the intercommunication filled the chamber 5 on the lateral wall of tube-shape metal sheath 1012, the width of business turn over mouth of pipe 7 is not less than the external diameter of sampling tube 4. And a positioning and pushing assembly 8 for positioning the sampling tube 4 and conveying the sampling tube 4 filled with the soil sample to the soil sample transfer mechanism 3 from the inlet and outlet pipe orifice 7 is arranged in the filling cavity 5.
The telescopic driving cylinder 1011 is a driving member having a reciprocating driving function such as a cylinder or an electric push rod, and the shapes of the cylindrical metal sheath 1012 and the filling chamber 5 are adapted to the shape of the sampling tube 4, but in order to prevent the soil sample from remaining at the corner of the sampling tube 4 and being difficult to clean, both the sampling tube 4 and the cylindrical metal sheath 1012 are cylindrical with one open end, and the filling chamber 5 is cylindrical with one open end adapted to the shape of the sampling tube 4.
The cylindrical metal sheath 1012 is alternatively butted with the soil sample transfer mechanism 3 and the supply mechanism 102 through the inlet and outlet pipe orifice 7, specifically, when frozen soil sampling is performed, the output port of the supply mechanism 102 is butted with the inlet and outlet pipe orifice 7, so that the output sampling pipe 4 enters the filling cavity 5 of the cylindrical metal sheath 1012 through the inlet and outlet pipe orifice 7. And after the sampling driving mechanism 101 drives the cylindrical metal sheath 1012 to be lifted out of the soil, the input port of the soil sample transfer mechanism 3 is switched to be in butt joint with the inlet and outlet pipe orifice 7, so that when the positioning and pushing assembly 8 pushes the sampling pipe 4 filled with the soil sample out of the inlet and outlet pipe orifice 7, the soil sample transfer mechanism 3 can receive the sampling pipe 4 filled with the soil sample. Subsequently, the soil sample transfer mechanism 3 transfers the sampling tube 4 containing the soil sample directly or indirectly by way of manual assistance to the microorganism extraction device 2, and at the same time, the input port of the soil sample transfer mechanism 3 is disengaged from the inlet/outlet nozzle 7, and the output port of the supply mechanism 102 is switched to be in butt joint with the inlet/outlet nozzle 7.
The cylindrical metal sheath 1012 comprises a pipe orifice protection section 1012a, an assembly section 1012b and a tail section 1012c which are sequentially connected and communicated with each other through the filling cavity 5, the tail section 1012c is connected with the telescopic driving cylinder 1011, the inlet and outlet pipe orifice 7 is arranged on the assembly section 1012b, a pipe orifice protection ring 9 for supporting and protecting the pipe orifice of the sampling pipe 4 is arranged on the inner wall of the pipe orifice protection section 1012a provided with the sampling port 6, and the inner diameter and the outer diameter of the pipe orifice protection ring 9 are not smaller than the inner diameter and the outer diameter of the sampling pipe 4.
The cylindrical metal sheath 1012 is made of steel and is cylindrical, the top of the pipe orifice protection ring 9 and the bottom of the flat channel 1021 are in the same horizontal plane or slightly lower than the bottom of the flat channel 1021, so that the sampling pipe 4 output through the flat channel 1021 can be smoothly conveyed to the pipe orifice protection ring 9, the pipe orifice at the bottom of the sampling pipe 4 is supported and protected through the pipe orifice protection ring 9, the sampling pipe 4 is prevented from being damaged by frozen earth and hard objects such as stones in the frozen earth due to the fact that the sampling pipe 4 is directly inserted into the frozen earth, recycling of the sampling pipe 4 is facilitated, and sampling failure caused by breakage of the sampling pipe 4 when the frozen earth is sampled is avoided.
The positioning and pushing assembly 8 comprises an electric push rod 801, an end positioning block 802 and a translation pushing plate 803, the translation pushing plate 803 is installed in the filling cavity 5, the end positioning block 802 is installed at one end of the translation pushing plate 803 facing to a tail section 1012c, the electric push rod 801 is installed on the tail section 1012c and is connected with the back of the translation pushing plate 803 opposite to the inlet and outlet nozzle 7, an electromagnet 804 is installed at the bottom of the end positioning block 802 facing to an assembling section 1012b, and the sampling tube 4 in the filling cavity 5 is pushed by the electromagnet 804 to abut against the nozzle protection ring 9.
The iron core of the electromagnet 804 contacts with the top of the sampling tube 4 through a rubber pad, when the sampling tube 4 is pulled out of frozen soil along with the cylindrical metal sheath 102 and is reset, the electromagnet 804 is electrified to enable the iron core abutting against the sampling tube 4 to retract, the positioning state of the sampling tube 4 is released, and then the electric push rod 801 pushes the sampling tube 4 filled with soil samples in the adjusting cavity 5 to the soil sample transfer mechanism 3 through pushing the translation push plate 803 from the inlet and outlet pipe opening 7.
The supply mechanism 102 includes a flat passage 1021 with one side open and a pushing cylinder 1022 installed in the other side of the flat passage 1021, and an abdicating gap 10 for the soil sample transfer mechanism 3 to transfer the sampling tube 4 is provided between the open side of the flat passage 1021 and the cylindrical metal sheath 1012.
Soil sample shifts mechanism 3 and changes over into external member 301 and transition and roll out external member 302 including the transition that moves in turn to in the clearance 10 of stepping down in turn over in the transition, the transition is changed over into external member 301 and is rolled out external member 302 and be connected through same guide plate 303, guide plate 303 coaxial arrangement has one end to be located the guide shaft 304 of stepping down the clearance 10 under, the other end transmission of guide shaft 304 is connected with and is installed servo motor 305 on the outer wall of flat passageway 1021, and install on the outer wall of flat passageway 1021 and be used for carrying out the support base 306 that supports guide shaft 304, run through on the support base 306 and set up with guide shaft 304 normal running fit's shaft hole.
The servo motor 305 intermittently drives the guide shaft 304 to rotate in a reciprocating manner, the guide disc 303 drives the installed transition switching sleeve piece 301 and the transition switching sleeve piece 302 to alternately enter the abdicating gap 10 under the driving of the guide shaft 304, when the flat channel 1021 conveys the sampling tube 4 to the filling cavity 5, the transition switching sleeve piece 301 moves to the abdicating gap 10 under the driving of the servo motor 305, the sampling tube 4 outputting the flat channel 1021 is transferred to the filling cavity 5 through the transition switching sleeve piece 301, similarly, when the cylindrical metal sheath 1012 is pulled out from the frozen soil and reset under the driving of the telescopic driving cylinder 1011, the transition switching sleeve piece 302 enters the abdicating gap 10 under the driving of the servo motor 305, when the sampling tube 4 is pushed out of the filling cavity 5 by the translation pushing plate 803, the transition switching sleeve piece 302 is received by the transition switching sleeve piece 302, and the transition switching sleeve piece 301 enters the abdicating gap 10 again, to prepare for transferring the next sampling tube 4 to the filling chamber 5 of the cylindrical metal sheath 1012, and the transition roll-out set 302 is returned to the original position and, at the same time, the sampling tube 4 with the soil sample is directly or indirectly transferred to the microorganism-extracting apparatus 2 by means of manual assistance to the microorganism-extracting apparatus 2.
The concentrative sleeve piece 301 and the concentrative sleeve piece 302 are matched through the arrangement of the abdicating gap 10 and the transition in and out alternately entering the abdicating gap 10, so that the sampling tube 4 enters the filling cavity 5 and moves out of the filling cavity 5 through the same inlet and outlet tube opening 7, the defect that the structural strength of the cylindrical metal sheath 1012 is influenced due to the fact that an opening is additionally formed in the cylindrical metal sheath 1012 is avoided, and the arrangement of the translation push plate 803 and the push driving part is facilitated.
Preferably, the transition turning-in kit 301 comprises a turning-in platform 3011 and an inverted U-shaped transition turning-in channel 3012, the turning-in platform 3011 is mounted on the side wall of the guide disc 303, one end of an opening of the transition turning-in channel 3012 is mounted on the front surface of the turning-in platform 3011 for supporting the sampling tube 4 to be transferred into the loading cavity 5, an input through hole 11 for guiding and limiting the sampling tube 4 is formed between the opening of the transition turning-in channel 3012 and the front surface of the turning-in platform 3011, and the sampling tube 4 output from the flat channel 1021 sequentially passes through the input through hole 11 and the inlet and outlet pipe orifice 7 to enter the loading cavity 5.
A plurality of sampling tubes 4 which are mutually attached and placed in a flat channel 1021 are pushed to a turning-in platform 3011 one by one under the driving of a pushing cylinder 1022, and along with the further pushing of the pushing cylinder 1022, the sampling tubes 4 positioned on the turning-in platform 3011 are pushed to a tube opening protection ring 9 in a filling cavity 5, namely, the turning-in platform 3011 is used as transition to transfer the sampling tubes 4 to the filling cavity 5 of a cylindrical metal sheath 1012, and the sampling tubes 4 are laterally limited through an input through hole 11 of the transition turning-in channel 3012, so that the situation that the sampling tubes 4 cannot enter the filling cavity 5 due to deviation from an inlet and outlet tube opening 7 when the sampling tubes 4 are conveyed into the filling cavity 5 through the turning-in platform 3011 is prevented.
Preferably, the transition roll-out kit 302 comprises a roll-out platform 3021 and a groove-shaped output arm 3022, one end of the groove-shaped output arm 3022 is mounted on the roll-out platform 3021, and an output groove 12 for receiving the sampling tube 4 pushed out by the translational push plate 803 is opened at a side of the groove-shaped output arm 3022 facing the inlet/outlet nozzle 7, when the groove-shaped output arm 3022 is reset, the sampling tube 4 filled with soil sample in the output groove 12 is butted onto the microorganism extracting apparatus 2 by a manual or external transfer device to extract microorganisms.
What is further optimized in the above embodiment is that the soil sample collecting device further comprises a housing 13 in which the soil sample collecting device 1, the soil sample transferring mechanism 3 and the microorganism extracting device 2 are installed, a mud scraping hole 1301 for the cylindrical metal sheath 1012 to pass through is formed at the bottom of the housing 13, and the inner diameter of the mud scraping hole 1301 is the same as the outer diameter of the cylindrical metal sheath 1012, so that the soil on the outer surface of the cylindrical metal sheath 1012 extracted from the soil is scraped through the housing 13 around the mud scraping hole 1301. The microorganism extraction device 2 is located behind the tubular metal sheath 1012 relative to the inlet/outlet pipe opening 7, one side of the housing 13 is penetrated and provided with a soil sample transfer outlet 1302 for exposing the groove-shaped output arm 3022, the housing 13 is penetrated and provided with a sampling pipe interface 1303, and the sampling pipe 4 in the output groove 12 of the groove-shaped output arm 3022 is butted to the sampling butt joint port of the microorganism extraction device 2 through the sampling pipe interface 1303 by means of manual transfer.
The invention also provides a sampling method of the frozen soil microorganism sampling device, which comprises the following steps:
s100, determining the positions and sampling depths of a plurality of sampling points in a frozen soil sampling area;
s200, starting a supply mechanism, wherein the supply mechanism conveys a plurality of sampling tubes stored in the supply mechanism one by one to a cylindrical metal sheath driven by a telescopic driving cylinder;
s300, aligning a sample inlet at the bottom of the cylindrical metal sheath with a sampling point, and setting the stroke of the telescopic driving cylinder according to the sampling depth of the corresponding sampling point;
s400, the telescopic driving cylinder drives the cylindrical metal sheath to be inserted into a soil layer with a corresponding depth of frozen soil according to a set stroke, so that soil below a corresponding sampling point enters a sampling tube from a sample inlet at the bottom of the cylindrical metal sheath, and extruded soil in the sampling tube is retained in the sampling tube in a tensioning mode;
s500, driving the cylindrical metal sheath to reset by the telescopic driving cylinder so as to expose the sampling tube with the pattern inside;
s600, the soil sample transfer mechanism transfers the sampling tube filled with the soil sample in the cylindrical metal sheath to the microorganism extraction device;
s700, taking out soil in the sampling pipe of the soil sample transfer mechanism by the microorganism extraction device and extracting to finish sampling of frozen soil microorganisms;
and S800, repeating S100-S700 until all the sampling points and the soil samples at the sampling depth are completely collected.
It is further optimized on the above embodiment that the soil sample transfer mechanism couples the sampling tube removed from the cylindrical metal sheath to the sampling docking port of the microorganism extraction device in a manually assisted manner, so as to simplify the structure of the device and save the device cost.
The above embodiments are only exemplary embodiments of the present application, and are not intended to limit the present application, and the protection scope of the present application is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present application and such modifications and equivalents should also be considered to be within the scope of the present application.
Claims (10)
1. The frozen soil microorganism sampling device is characterized by comprising a soil sample collecting device (1) and a microorganism extracting device (2), wherein the soil sample collecting device (1) conveys collected frozen soil to the microorganism extracting device (2) through a soil sample transferring mechanism (3) to extract microorganisms;
soil sample collection system (1) is including sample actuating mechanism (101) and supply mechanism (102), supply mechanism (102) storage has a plurality of sampling tubes (4), just supply mechanism (102) will be a plurality of sampling tube (4) one by one to sample actuating mechanism (101) supply, sample actuating mechanism (101) with sampling tube (4) that supply mechanism (102) carried are assembled, and sample actuating mechanism (101) is to rather than assembling sampling tube (4) carry out reciprocating drive, so that sampling tube (4) are inserted and are taken a sample and extract from the soil layer in the soil layer, soil sample transfer mechanism (3) will sample actuating mechanism (101) break away from extract in the soil layer sampling tube (4) that are equipped with the soil sample to microorganism extraction element (2) carry.
2. The frozen soil microorganism sampling device according to claim 1, wherein the sampling driving mechanism (101) comprises a telescopic driving cylinder (1011) and a cylindrical metal sheath (1012) mounted on the telescopic driving cylinder (1011), a filling cavity (5) for the sampling tube (4) to be filled is arranged in the cylindrical metal sheath (1012), the top end of the cylindrical metal sheath (1012) is connected with the telescopic driving cylinder (1011), the filling cavity (5) penetrates through the bottom of the cylindrical metal sheath (1012) to form a sample inlet (6) for a soil sample to enter the sampling tube (4), an inlet and outlet pipe orifice (7) which is communicated with the filling cavity (5) and is used for filling the sampling pipe (4) is arranged on the side wall of the cylindrical metal sheath (1012), the width of the inlet and outlet pipe orifice (7) is not less than the outer diameter of the sampling pipe (4);
install in filling chamber (5) and be used for sampling tube (4) are fixed a position and will be equipped with soil sample sampling tube (4) by business turn over mouth of pipe (7) to soil sample transfer mechanism (3) carry out the location and propelling movement subassembly (8) of carrying.
3. The frozen soil microorganism sampling device of claim 2, wherein the cylindrical metal sheath (1012) comprises a nozzle protection section (1012a), an assembly section (1012b) and a tail section (1012c) which are connected in sequence and communicated with each other through the filling cavity (5), the tail section (1012c) is connected with the telescopic driving cylinder (1011), the inlet and outlet nozzle (7) is arranged on the assembly section (1012b), the inner wall of the nozzle protection section (1012a) provided with the sample inlet (6) is provided with a nozzle protection ring (9) for supporting and protecting the nozzle of the sampling tube (4), and the inner diameter and the outer diameter of the nozzle protection ring (9) are not less than the inner diameter and the outer diameter of the sampling tube (4).
4. The frozen soil microorganism sampling device according to claim 2, wherein the positioning and pushing assembly (8) comprises an electric push rod (801), an end positioning block (802) and a translation push plate (803), the translational push plate (803) is installed in the filling cavity (5), the end positioning block (802) is installed at one end of the translational push plate (803) facing the tail section (1012c), the electric push rod (801) is arranged on the tail section (1012c) and is connected with the back of the translation push plate (803) relative to the inlet and outlet pipe orifice (7), an electromagnet (804) is arranged at the bottom of the end positioning block (802) facing the assembling section (1012b), the sampling tube (4) in the filling cavity (5) is pressed against the nozzle protection ring (9) by the push of the electromagnet (804).
5. The frozen soil microorganism sampling device according to claim 1, wherein the supply mechanism (102) comprises a flat channel (1021) with one side open and a pushing cylinder (1022) installed in the other side of the flat channel (1021), and a yielding gap (10) for the soil sample transfer mechanism (3) to transfer the sampling tube (4) is arranged between the open side of the flat channel (1021) and the cylindrical metal sheath (1012);
the soil sample transfer mechanism (3) comprises a transition transfer sleeve (301) for transferring the empty sampling tube (4) to the filling cavity (5) and a transition transfer sleeve (302) for receiving the sampling tube (4) pushed out by the translation pushing plate (803), the transition transfer sleeve (301) and the transition transfer sleeve (302) are connected through the same guide disc (303) and alternately move to the abdicating gap (10), the guide disc (303) is coaxially provided with a guide shaft (304) with one end positioned right below the abdicating gap (10), the other end of the guide shaft (304) is in transmission connection with a servo motor (305) arranged on the outer wall of the flat channel (1021), and the outer wall of the flat channel (1021) is provided with a support base (306) for supporting the guide shaft (304), the supporting base (306) is provided with a shaft hole which is in running fit with the guide shaft (304).
6. The frozen soil microorganism sampling device according to claim 5, wherein the transition transfer-in kit (301) comprises a transfer-in platform (3011) and a transition transfer-in channel (3012) in an inverted 'U' shape, the turning-in platform (3011) is installed on the side wall of the guide disc (303), one end of the opening of the transition turning-in channel (3012) is installed on the front surface of the turning-in platform (3011) for supporting the sampling tube (4) to be transferred into the filling cavity (5), an input through hole (11) used for guiding and limiting the sampling tube (4) is formed between the opening of the transition transfer-in passage (3012) and the front face of the transfer-in platform (3011), and the sampling tube (4) output from the flat passage (1021) sequentially passes through the input through hole (11) and the inlet and outlet pipe orifice (7) to enter the filling cavity (5).
7. The frozen soil microorganism sampling device according to claim 6, wherein the transition roll-out kit (302) comprises a roll-out platform (3021) and a trough-shaped output arm (3022), one end of the trough-shaped output arm (3022) is mounted on the roll-out platform (3021), and the side of the trough-shaped output arm (3022) facing the access nozzle (7) is opened with an output groove (12) for receiving the sampling tube (4) pushed out by the translational push plate (803).
8. The frozen soil microorganism sampling device according to claim 6, further comprising a housing (13) in which the soil sample collection device (1), the soil sample transfer mechanism (3) and the microorganism extraction device (2) are installed, wherein a mud scraping hole (1301) for the cylindrical metal sheath (1012) to pass through is formed at the bottom of the housing (13);
the microorganism extraction device (2) is positioned behind the cylindrical metal sheath (1012) relative to the inlet and outlet pipe orifice (7), one side of the shell (13) is penetrated and provided with a soil sample transferring outlet (1302) for exposing the groove-shaped output arm (3022), a sampling pipe interface (1303) is penetrated and provided on the shell (13), and the sampling pipe (4) in the output groove (12) of the groove-shaped output arm (3022) is butted to a sampling butt joint port of the microorganism extraction device (2) through the sampling pipe interface (1303) under the driving of an external power source.
9. A sampling method for the frozen soil microorganism sampling apparatus according to any one of claims 1 to 8, comprising:
s100, determining the positions and sampling depths of a plurality of sampling points in a frozen soil sampling area;
s200, starting a supply mechanism, wherein the supply mechanism conveys a plurality of sampling tubes stored in the supply mechanism one by one to a cylindrical metal sheath driven by a telescopic driving cylinder;
s300, aligning a sample inlet at the bottom of the cylindrical metal sheath with a sampling point, and setting the stroke of the telescopic driving cylinder according to the sampling depth corresponding to the sampling point;
s400, the telescopic driving cylinder drives the cylindrical metal sheath to be inserted into a soil layer with a corresponding depth of frozen soil according to a set stroke, so that soil below a corresponding sampling point enters the sampling tube from a sample inlet at the bottom of the cylindrical metal sheath, and extruded soil in the sampling tube is retained in the sampling tube in a tensioning mode;
s500, the telescopic driving cylinder drives the cylindrical metal sheath to reset so as to expose the sampling tube with the pattern inside;
s600, the sampling tube filled with the soil sample in the cylindrical metal sheath is transferred to a microorganism extraction device by a soil sample transfer mechanism;
s700, taking out soil in the sampling pipe of the soil sample transfer mechanism by the microorganism extraction device and extracting to finish sampling of frozen soil microorganisms;
and S800, repeating S100-S700 until all the sampling points and the soil samples at the sampling depth are completely collected.
10. The sampling method of a frozen soil microorganism sampling device according to claim 9, wherein the soil sample transfer mechanism manually assists in docking the sampling tube removed from the cylindrical metal sheath to the sampling docking port of the microorganism extraction device.
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