CN113318144A - Preparation method of isoflavone and saponin in soybean - Google Patents
Preparation method of isoflavone and saponin in soybean Download PDFInfo
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- CN113318144A CN113318144A CN202110679318.7A CN202110679318A CN113318144A CN 113318144 A CN113318144 A CN 113318144A CN 202110679318 A CN202110679318 A CN 202110679318A CN 113318144 A CN113318144 A CN 113318144A
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- soybean
- drying
- solvent
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- isoflavone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a preparation method of isoflavone and saponin in soybean, belonging to the technical field of medicine and comprising the following steps: heating and refluxing soybeans for 2 times by using a mixed solution of ethyl acetate and one of methanol, ethanol or acetone as an extraction solvent, combining the extracting solutions, concentrating to obtain a concentrated solution 1, standing the concentrated solution 1 overnight, adding water, heating for dispersion again, cooling overnight, performing centrifugal separation, drying biscuits to obtain high-content soybean isoflavone, drying extraction residues, adding high-concentration methanol or ethanol for extraction for 2 times, recovering the solvent from the extracting solution under reduced pressure, adding acid to adjust the pH value to 3-4, standing overnight, performing centrifugal separation, and drying the biscuits to obtain the soybean saponin. The technology improves the utilization rate of the soybeans by adopting a mode of extracting and separating a plurality of products step by step, thereby greatly improving the enterprise profits.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a preparation method of isoflavone and saponin in soybeans.
Background
The soybean contains rich functional components, especially special isoflavone and saponin as main effective components, and has anticancer and antiaging effects. The soybean isoflavone in the soybean is a general term of isoflavone, generally comprises 6 components of daidzin, glycitin, genistin and aglycone of the three components, and the daidzin is a more complex saponin mixture. In recent years, soybean has been increasingly emphasized. The soybean product also becomes one of the most advocated health-care foods by people in the world today. Soy isoflavones are also referred to as "botanical natural estrogens" because of their significant estrogen-like effects. The extraction and separation technology of active substances and the raw material resource problem of medicinal plants are key factors for restricting the industrialization of the active substances. On one hand, the soybean contains less active substances such as soybean isoflavone, soybean saponin and the like, the extraction and separation are difficult, the cost of the technology for extracting the effective components and the raw materials for extracting the single medicinal component by taking the soybean as the raw material is high at present, so that the enterprise engaged in the extraction of the natural active substances of the soybean has a tiny profit.
Disclosure of Invention
The invention aims to provide a preparation method of isoflavone and saponin in soybean aiming at the existing problems.
The invention is realized by the following technical scheme:
a method for preparing isoflavone and saponin in soybean comprises the following steps:
(1) heating and refluxing defatted soybean with mixed solvent for 2 times, mixing extractive solutions, and collecting soybean residue;
(2) concentrating the combined extract in the step (1) to obtain a concentrated solution 1, standing the concentrated solution 1 overnight, adding water, heating again for dispersion, cooling overnight, centrifuging, and drying to obtain high-content soybean isoflavone;
(3) heating the soybean residue collected in the step (1) to recover the solvent, drying, adding high-concentration alcohol, performing reflux extraction for 2 times, recovering the solvent from the obtained extracting solution under reduced pressure, adding acid to adjust the pH value to 3-4, standing overnight, performing centrifugal separation, drying the cake to obtain soyasaponin, and collecting the extracted soybean for later use;
(4) and (4) heating the extracted soybeans collected in the step (4) to recover the solvent, and drying to obtain the soybeans for the feed.
Further, the mixed solvent in the step (1) is a mixed solution of ethyl acetate and one of methanol, ethanol or acetone.
Further, the high-concentration alcohol in the step (3) is methanol or ethanol, and the concentration is more than or equal to 85%.
Compared with the prior art, the invention has the following advantages:
the technology improves the utilization rate of the soybeans by adopting a mode of extracting and separating a plurality of products step by step, thereby greatly improving the enterprise profits.
Drawings
Fig. 1 is a flow chart of a method for preparing isoflavones and saponins from soybeans provided by the present application.
Detailed Description
Implementation mode one
Adding methanol into 300kg of defatted soybean germs: boiling and refluxing for 2h with 2400L mixed solvent of ethyl acetate 1:1, repeating for 2 times, mixing extractive solutions to obtain a liquid A containing soybean isoflavone, heating the extracted soybean material to recover the solvent, adding 2400L 90% methanol for refluxing and extracting for 2 times, mixing the extractive solutions to obtain a liquid B containing soybean saponin, heating the extracted soybean material to recover the solvent, drying to obtain soybean for feed, separately concentrating the liquids A and B to recover the solvents to obtain aqueous solutions C and D, standing for 24h, adding water, centrifuging, drying to obtain 65% soybean isoflavone, adding hydrochloric acid to the solution D containing soybean saponin to adjust pH to 3, standing for 24h, centrifuging, and drying to obtain 72% soybean saponin.
Second embodiment
Adding methanol into 300kg of defatted soybean germs: boiling and refluxing for 2h with 2400L mixed solvent of 2:3 ethyl acetate, repeating for 2 times, mixing extractive solutions to obtain a liquid A containing soybean isoflavone, heating the extracted soybean material to recover the solvent, adding 2400L 90% methanol for refluxing and extracting for 2 times, mixing the extractive solutions to obtain a liquid B containing soybean saponin, heating the extracted soybean material to recover the solvent, drying to obtain soybean for feed, separately concentrating the liquids A and B to recover the solvents to obtain aqueous solutions C and D, standing for 24h, adding water, centrifuging, drying to obtain soybean isoflavone with the content of 75%, adding hydrochloric acid to the solution D containing soybean saponin to adjust the pH to 3, standing for 24h, centrifuging, and drying to obtain soybean saponin with the content of 78%.
Third embodiment
Adding 300kg of defatted soybean germ into ethanol: boiling and refluxing for 2h with 2400L mixed solvent of ethyl acetate 1:1, repeating for 2 times, mixing extractive solutions to obtain a liquid A containing soybean isoflavone, heating the extracted soybean material to recover the solvent, adding 2400L 90% ethanol, refluxing for 2 times, mixing the extractive solutions to obtain a liquid B containing soybean saponin, heating the extracted soybean material to recover the solvent, drying to obtain soybean for feed, separately concentrating the liquids A and B to recover the solvents to obtain aqueous solutions C and D, standing for 24h, adding water, centrifuging, drying to obtain soybean isoflavone with the content of 82%, adding hydrochloric acid to the solution D containing soybean saponin to adjust the pH to 3, standing for 24h, centrifuging, and drying to obtain soybean saponin with the content of 76%.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The use of the phrase "comprising one of the elements does not exclude the presence of other like elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A method for preparing isoflavone and saponin in soybean is characterized by comprising the following steps:
(1) heating and refluxing defatted soybean with mixed solvent for 2 times, mixing extractive solutions, and collecting soybean residue;
(2) concentrating the combined extract in the step (1) to obtain a concentrated solution 1, standing the concentrated solution 1 overnight, adding water, heating again for dispersion, cooling overnight, centrifuging, and drying to obtain high-content soybean isoflavone;
(3) heating the soybean residue collected in the step (1) to recover the solvent, drying, adding high-concentration alcohol, performing reflux extraction for 2 times, recovering the solvent from the obtained extracting solution under reduced pressure, adding acid to adjust the pH value to 3-4, standing overnight, performing centrifugal separation, drying the cake to obtain soyasaponin, and collecting the extracted soybean for later use;
(4) and (4) heating the extracted soybeans collected in the step (4) to recover the solvent, and drying to obtain the soybeans for the feed.
2. The method for preparing isoflavones and saponins in soybeans according to claim 1, wherein the mixed solvent in step (1) is a mixed solution of ethyl acetate and one of methanol, ethanol and acetone.
3. The method for preparing isoflavones and saponins in soybeans according to claim 1, wherein the high-concentration alcohol in the step (3) is methanol or ethanol, and the concentration is more than or equal to 85%.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110679318.7A CN113318144A (en) | 2021-06-18 | 2021-06-18 | Preparation method of isoflavone and saponin in soybean |
| CN202210128962.XA CN114306421A (en) | 2021-06-18 | 2022-02-11 | Method for preparing isoflavone and saponin in soybean |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110679318.7A CN113318144A (en) | 2021-06-18 | 2021-06-18 | Preparation method of isoflavone and saponin in soybean |
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| Publication Number | Publication Date |
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| CN113318144A true CN113318144A (en) | 2021-08-31 |
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| CN202110679318.7A Withdrawn CN113318144A (en) | 2021-06-18 | 2021-06-18 | Preparation method of isoflavone and saponin in soybean |
| CN202210128962.XA Pending CN114306421A (en) | 2021-06-18 | 2022-02-11 | Method for preparing isoflavone and saponin in soybean |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210128962.XA Pending CN114306421A (en) | 2021-06-18 | 2022-02-11 | Method for preparing isoflavone and saponin in soybean |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113861152A (en) * | 2021-11-04 | 2021-12-31 | 山东东宇生态科技有限公司 | Preparation method of soybean isoflavone |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1109682C (en) * | 2000-03-31 | 2003-05-28 | 姜浩奎 | Process for extracting glycitin and soybean saponin |
| CN1096456C (en) * | 2000-06-08 | 2002-12-18 | 蒋宇扬 | Process for continuously extracting glycitin, olegose and saponin |
| CN1300135C (en) * | 2005-02-07 | 2007-02-14 | 黑龙江双河松嫩大豆生物工程有限责任公司 | Method for preparing soybean isoflavone, soybean spanin, oligosaccharide and soybean concentrated protein |
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2021
- 2021-06-18 CN CN202110679318.7A patent/CN113318144A/en not_active Withdrawn
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2022
- 2022-02-11 CN CN202210128962.XA patent/CN114306421A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113861152A (en) * | 2021-11-04 | 2021-12-31 | 山东东宇生态科技有限公司 | Preparation method of soybean isoflavone |
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| CN114306421A (en) | 2022-04-12 |
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Application publication date: 20210831 |