CN113281275A - Detection method based on absorption spectrum technology - Google Patents
Detection method based on absorption spectrum technology Download PDFInfo
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- CN113281275A CN113281275A CN202110351653.4A CN202110351653A CN113281275A CN 113281275 A CN113281275 A CN 113281275A CN 202110351653 A CN202110351653 A CN 202110351653A CN 113281275 A CN113281275 A CN 113281275A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The invention provides a detection method based on an absorption spectrum technology, which comprises the following steps: (A1) the detection light emitted from the light source passes through the light filtering component and the detection cells respectively having different concentrations CiThe detector receives the optical signals passing through the detection cell respectively and converts the optical signals into electric signals UsiI is 1,2 … n, n is an integer not less than 2; (A2) the analysis unit processes the electrical signal UsiRespectively obtaining absorbances corresponding to different concentrations
U0An electric signal corresponding to the initial intensity of the detection light, m is a coefficient, and m belongs to (0, 1); (A3) obtaining the absorbance A at different values of miFor the absorbance AiAnd concentration CiPerforming goodness-of-fit calculation to obtain m value m when correlation coefficient is closest to 10(ii) a (A4) For the liquid to be measured, the detector obtains an electric signal Us(ii) a (A5) The analysis unit obtains the absorbance
And obtaining the parameters of the liquid to be measured. The invention has the advantages of accurate detection, high sensitivity and the like.
Description
Technical Field
The invention relates to spectral analysis, in particular to a detection method based on an absorption spectrum technology.
Background
Stray light is light in other spectral bands outside the set wavelength or band received by the detector. Stray light is generated due to processing and manufacturing errors of the optical filter, diffuse reflection on the surface of the optical filter, and the like. Therefore, in the current absorbance detection system based on the optical filter, the interference of stray light generally exists, and the concentration of a sample and the measured absorbance deviate from the lambert beer law. Some methods suppress the influence of stray light by performing multi-point correction on a detection system and fitting a polynomial, and the method needs multi-point calibration before each measurement, thereby greatly increasing time cost and workload. Still other approaches increase the hermetic opacity of the detection path, but do not significantly suppress stray light.
In summary, the problem of the absorbance detection system based on the optical filter is that the sample concentration and the measured absorbance of the photometric detection system deviate from the lambert beer law due to the processing technology and manufacturing error of the optical filter and the stray light caused by the diffuse reflection on the surface of the optical filter, and the test result of the absorbance detection system based on the optical filter is not accurate enough and the sensitivity is not high enough.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a detection method based on an absorption spectrum technology.
The purpose of the invention is realized by the following technical scheme:
the detection method based on the absorption spectrum technology comprises the following steps:
(A1) the detection light emitted from the light source passes through the light filtering component and the detection cells respectively having different concentrations CiThe detector receives the optical signals passing through the detection cell respectively and converts the optical signals into electric signals UsiI is 1, 2. cndot. n, n is an integer not less than 2;
(A2) the analysis unit processes the electrical signal UsiRespectively obtaining absorbances corresponding to different concentrations
U0For the electric signal corresponding to the initial intensity of the detection light, m is equal to [0,1 ]]；
(A3) Obtaining the absorbance A at different values of miFor the absorbance AiAnd is concentratedDegree CiPerforming goodness-of-fit calculation to obtain m value m when correlation coefficient is closest to 10；
(A4) For the liquid to be measured, the detector obtains an electric signal Us；
(A5) The analysis unit obtains the absorbance
And obtaining the parameters of the liquid to be detected by using an absorption spectrum technology.
Compared with the prior art, the invention has the beneficial effects that:
find value m0And is used for correcting the absorbance, thereby improving the accuracy of subsequent detection and improving the detection sensitivity;
experimental results show that the detection accuracy can be obviously improved.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only for illustrating the technical solutions of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a flow chart of a detection method based on absorption spectroscopy according to an embodiment of the invention;
FIG. 2 shows a search value m according to an embodiment of the invention0Is described.
Detailed Description
Fig. 1-2 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and use the invention. Some conventional aspects have been simplified or omitted for the purpose of explaining the technical solution of the present invention. Those skilled in the art will appreciate variations or substitutions from these embodiments that will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but only by the claims and their equivalents.
Example 1:
fig. 1 schematically shows a flow chart of a detection method based on an absorption spectrum technology according to an embodiment of the present invention, and as shown in fig. 1, the detection method based on the absorption spectrum technology includes the following steps:
(A1) the detection light emitted from the light source passes through the light filtering component and the detection cells respectively having different concentrations CiThe detector receives the optical signals passing through the detection cell respectively and converts the optical signals into electric signals UsiI is 1, 2. cndot. n, n is an integer not less than 2;
(A2) the analysis unit processes the electrical signal UsiRespectively obtaining absorbances corresponding to different concentrations
U0For the electric signal corresponding to the initial intensity of the detection light, m is equal to [0,1 ]]；
(A3) Obtaining the absorbance A at different values of miFor the absorbance AiAnd concentration CiPerforming goodness-of-fit calculation to obtain m value m when correlation coefficient is closest to 10；
(A4) For the liquid to be measured, the detector obtains an electric signal Us；
(A5) The analysis unit obtains the absorbance
And obtaining the parameters of the liquid to be detected by using an absorption spectrum technology.
To shorten the search value m0And furthermore, the values of different m values are assigned to m according to the step length.
Example 2:
the detection method based on the absorption spectrum technology in the embodiment 1 of the invention is applied to water sample detection.
In this application example, the detection method based on the absorption spectrum technology includes the following steps:
(A1) the detection light in ultraviolet band emitted from the light source sequentially passes through the filter component (such as optical filter) and the detection cellRespectively having different total nitrogen concentration CiThe standard solutions of (1) are 0, 20%, 50%, 80%, 100% gradient standard solutions of concentration C, namely 5 different concentrations of standard solutions; pure water is adopted as 0 gradient standard solution with concentration C;
the detector receives the optical signals passing through the detection cell and respectively converts the optical signals into electric signals Usi，i＝1,2···5；
(A2) The analysis unit processes the electrical signal UsiRespectively obtaining absorbances corresponding to different concentrations
U0For the electric signal corresponding to the initial intensity of the detection light, m is equal to [0,1 ]]；
(A3) As shown in FIG. 2, the absorbances A at different values of m (increasing step size b) were obtainediFor the absorbance AiAnd concentration CiCalculating the goodness of fit to obtain the value m when the linear correlation coefficient is closest to 1, and recording the value m as m0；
(A4) For a water sample to be detected, the detector obtains an electric signal Us；
(A5) The analysis unit obtains the absorbance
Obtaining the total nitrogen concentration in the water sample to be detected by utilizing an ultraviolet differential absorption spectrum technology;
in this embodiment, the experimental results obtained after the detection method is adopted are as follows:
for comparison, experimental results obtained without the detection method;
the comparative results are shown in the following table
From the comparison, the accuracy and the sensitivity of total nitrogen measurement are improved by adopting the detection method.
Claims (6)
1. The detection method based on the absorption spectrum technology comprises the following steps:
(A1) the detection light emitted from the light source passes through the light filtering component and the detection cells respectively having different concentrations CiThe detector receives the optical signals passing through the detection cell respectively and converts the optical signals into electric signals UsiI is 1, 2. cndot. n, n is an integer not less than 2;
(A2) the analysis unit processes the electrical signal UsiRespectively obtaining absorbances corresponding to different concentrations
U0For the electric signal corresponding to the initial intensity of the detection light, m is equal to [0,1 ]]；
(A3) Obtaining the absorbance A at different values of miFor the absorbance AiAnd concentration CiPerforming goodness-of-fit calculation to obtain m value m when correlation coefficient is closest to 10；
(A4) For the liquid to be measured, the detector obtains an electric signal Us；
(A5) The analysis unit obtains the absorbance
And obtaining the parameters of the liquid to be measured by using an absorption spectrum technology.
2. The absorption spectroscopy-based detection method of claim 1, wherein the different values of m are valued in such a way that m is assigned according to a step size.
3. The absorption spectroscopy-based detection method of claim 1, wherein the correlation coefficient is a linear correlation coefficient.
4. The method according to claim 1, wherein the filter device is a filter, and the detecting light passes through the filter and the detecting cell sequentially.
5. The method according to claim 1, wherein the liquid to be detected is a water sample, the parameter is ammonia nitrogen, and the wavelength of the detection light is in an ultraviolet band.
6. The absorption spectroscopy-based detection method of claim 1, wherein the absorption spectroscopy technique is a differential absorption spectroscopy technique.
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| CN202110351653.4A CN113281275A (en) | 2021-03-31 | 2021-03-31 | Detection method based on absorption spectrum technology |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5042946A (en) * | 1990-05-02 | 1991-08-27 | Hitachi, Ltd. | Atomic absorption spectrophotometric method and apparatus |
| JP2003014628A (en) * | 2001-07-02 | 2003-01-15 | Ishikawajima Harima Heavy Ind Co Ltd | Calibration method for quantitative analysis |
| CN102980864A (en) * | 2012-12-06 | 2013-03-20 | 力合科技（湖南）股份有限公司 | Spectrophotometric detection device and detection method thereof |
| CN111504925A (en) * | 2020-04-29 | 2020-08-07 | 安徽岩芯光电技术有限公司 | Gas detection device and method |
-
2021
- 2021-03-31 CN CN202110351653.4A patent/CN113281275A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5042946A (en) * | 1990-05-02 | 1991-08-27 | Hitachi, Ltd. | Atomic absorption spectrophotometric method and apparatus |
| JP2003014628A (en) * | 2001-07-02 | 2003-01-15 | Ishikawajima Harima Heavy Ind Co Ltd | Calibration method for quantitative analysis |
| CN102980864A (en) * | 2012-12-06 | 2013-03-20 | 力合科技（湖南）股份有限公司 | Spectrophotometric detection device and detection method thereof |
| CN111504925A (en) * | 2020-04-29 | 2020-08-07 | 安徽岩芯光电技术有限公司 | Gas detection device and method |
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