CN113265021A - Preparation method and application of iron-based nano enzyme hydrogel - Google Patents
Preparation method and application of iron-based nano enzyme hydrogel Download PDFInfo
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- CN113265021A CN113265021A CN202110604031.8A CN202110604031A CN113265021A CN 113265021 A CN113265021 A CN 113265021A CN 202110604031 A CN202110604031 A CN 202110604031A CN 113265021 A CN113265021 A CN 113265021A
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- OIBVSTUGLWGXHJ-UHFFFAOYSA-N n-prop-1-en-2-ylprop-2-enamide Chemical compound CC(=C)NC(=O)C=C OIBVSTUGLWGXHJ-UHFFFAOYSA-N 0.000 claims description 3
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Abstract
The invention discloses a preparation method and application of an iron-based nanoenzyme hydrogel, which comprises the steps of dissolving NIPAAM, AAM, DMPA and MBA in deionized water to obtain a mixed solution I; dispersing the iron-based nanoenzyme in deionized water, slowly and dropwise adding the iron-based nanoenzyme into the solution I, and uniformly dispersing to obtain a dispersion liquid II; mixing Tetramethylethylenediamine (TMEDA) and ammonium persulfate [ (NH)4)2S2O8]Respectively adding the mixture into the dispersion liquid II, uniformly mixing to obtain a mixed solution III, pouring the mixed solution III into a hydrogel forming die, and standing for 3 hours at room temperature. Washing off residual TMEDA and (NH) after hydrogel forming4)2S2O8Then, the hydrogel is soaked in tert-butyl alcohol to replace water in the hydrogel. And finally, extracting the hydrogel with liquid nitrogen and freeze-drying to obtain the iron-based nano enzyme hydrogel. The iron-based nanoenzyme hydrogel provided by the invention has peroxidase activity and has the effect of capturing bacteria and healing wounds.
Description
Technical Field
The invention relates to the technical field of nano material mimic enzyme, in particular to an iron-based nano enzyme hydrogel and a preparation method and antibacterial application thereof.
Background
The nano enzyme is a nano material with natural enzyme catalytic activity, and the catalytic activity of the nano enzyme is from a special nano structure of the nano enzyme, so that a catalytic functional group or natural enzyme is not required to be additionally introduced. The development of the nanoenzyme provides a chance for developing a new antibacterial approach and method, because the nanoenzyme has the capacity of regulating the free radical level of Reactive Oxygen Species (ROS), breaks the balance of the ROS, further destroys the integrity of cell membranes or biological membranes, degrades nucleic acid, inactivates various proteins, and finally initiates the violent change of bacterial morphology and death, which is fundamentally different from the traditional antibiotics in antibacterial mechanism, thereby avoiding the generation of bacterial drug resistance.
However, almost all nanoenzymes do not interact efficiently with bacteria, and ROS have the inherent disadvantages of short lifetime (less than 200ns) and diffusion distance (about 20 nm). Therefore, there is a need to solve these problems to facilitate the application of nanoenzymes in the antibacterial field. Hydrogels have attracted a great deal of attention in the biomedical community because of their unique physicochemical properties. The hydrogel surface has positive charges and a macroporous structure, and can effectively capture bacteria and fix active oxygen substances. Hydrogels also have good biocompatibility and biodegradability, and can absorb and retain large amounts of water, providing a moist environment. Based on these unique properties, some hydrogels with antimicrobial properties have been used to heal wounds. In addition, the iron-based nanoenzyme has peroxidase-like (POD) activity, can induce hydroxyl radicals of hydrogen peroxide, and destroys the integrity of bacteria. Therefore, the hydrogel prepared by the in-situ free radical polymerization method coats the iron-based nanoenzyme, so that bacteria can be captured and limited in the damage range of ROS, and finally, the bacteria are killed.
Disclosure of Invention
Based on the needs of the prior art, one of the objectives of the present invention is to provide a method for preparing an iron-based nanoenzyme hydrogel; the other object of the invention is to provide an antibacterial application of the iron-based nanoenzyme hydrogel. In order to achieve the above purpose, the invention provides the following technical scheme through research:
the invention firstly provides a preparation method of an iron-based nanoenzyme hydrogel, which comprises the following steps:
1) sequentially adding N-isopropenylacrylamide (NIPAAM), acrylamide (AAM), N- (3-Dimethylaminopropyl) Methacrylamide (DMPA) and N, N-Methylene Bisacrylamide (MBA) into deionized water, and performing ultrasonic treatment until the N-isopropenylacrylamide (NIPAAM), the acrylamide (AAM), the N- (3-Dimethylaminopropyl) Methacrylamide (DMPA) and the N, N-Methylene Bisacrylamide (MBA) are completely dissolved to obtain a mixed solution I;
2) dispersing the iron-based nanoenzyme in deionized water, slowly adding the iron-based nanoenzyme into the solution I dropwise, and continuing to perform ultrasonic treatment until the solution I is uniformly dispersed to obtain a dispersion liquid II;
3) mixing Tetramethylethylenediamine (TMEDA) and ammonium persulfate [ (NH)4)2S2O8]Respectively adding the mixture into the dispersion liquid II, carrying out ultrasonic treatment until the mixture is completely uniform to obtain a mixed solution III, pouring the mixed solution III into a hydrogel forming mould, and standing the mixed solution for 3 hours at room temperature;
4) to be condensed with waterAfter the glue is formed, the glue is transferred into ultrapure water for soaking, and residual TMEDA and (NH) are washed off4)2S2O8Then soaking the hydrogel in tert-butyl alcohol to replace the water in the hydrogel;
5) extracting the hydrogel in liquid nitrogen for two or three times, and freeze-drying in a freeze dryer to obtain the iron-based nano enzyme hydrogel with the macroporous structure.
Specifically, the method comprises the following steps: the hydrogel is prepared by an in-situ free radical polymerization method, and tests verify that the optimal addition amount of each component and the optimal dropping speed of the iron-based nanoenzyme dispersion liquid, so that the hydrogel is formed while waste is avoided. In addition, the addition of the crosslinking agent TMEDA needs to be strictly controlled, the pore structure is compact and narrow due to too much crosslinking agent TMEDA, and the hydrogel is difficult to form and cannot be subjected to the next operation if the addition amount is small.
a. Iron-based nanoenzyme:
fe in a large number of iron atoms on the surface of the iron-based nanoenzyme2+/Fe3+The conversion between them is the key to ensure the enzymatic activity. The peroxidase simulation activity of the iron-based nanoenzyme under the acidic condition accords with the Fenton mechanism, and Fe3+And H2O2The reaction generates hydroxyl radicals. The catalytic activity of the iron-based nano-enzyme can also be adjusted through the size, the shape, the structure and the surface modification of the nano-material. The iron-based nanoenzyme mainly comprises iron-based oxide such as Fe2O3、Fe3O4、FeMnO3、Fe2(MoO4)3FeOOH and FePO4And the like.
b. Preparing the iron-based nano enzyme hydrogel by using an in-situ free radical polymerization method:
sequentially adding NIPAAM, AAM, DMPA and MBA into deionized water, performing ultrasonic treatment until complete dissolution to obtain a mixed solution I, dispersing the iron-based nanoenzyme into the deionized water, slowly dropwise adding the solution I, and continuously performing ultrasonic treatment until uniform dispersion is achieved to obtain a dispersion liquid II, a certain volume of TMEDA and (NH)4)2S2O8Respectively adding the aqueous solution into the dispersion liquid II, and carrying out ultrasonic treatment until the aqueous solution is completely uniform to obtain a mixed solution III; the mixed solution III was poured into a hydrogel-forming mold and left at room temperature for 3 hours. To be hydratedAfter forming, the mixture is transferred into ultrapure water for soaking, and residual TMEDA and (NH) are washed off4)2S2O8Then soaking the hydrogel in tert-butyl alcohol to replace the water in the hydrogel. And finally, extracting the hydrogel in liquid nitrogen for two or three times, and freeze-drying in a freeze dryer to obtain the iron-based nano enzyme hydrogel with the macroporous structure.
In one embodiment of the invention, in the step 1), the mass ratio of NIPAAM, AAM, DMPA, MBA and deionized water is 1: 15-20, 1: 50-55, 1: 150-155.
In one embodiment according to the invention, in the step 2), the mass fraction of the iron-based nanoenzyme dispersion is 0.1-2.0%.
In one embodiment of the invention, in the step 2), the acceleration of the iron-based nanoenzyme dispersed liquid drop is 10-100 μ L/s.
In one embodiment according to the invention, in step 3), (NH)4)2S2O8The mass fraction of the aqueous solution is 1-10%.
In one embodiment according to the invention, in step 3), (NH)4)2S2O8The volume ratio of the aqueous solution to the TMEDA is 1: 0.5-3, and more preferably 1: 0.8-1.5.
In one embodiment according to the invention, in step 3), the volume ratio of the dispersion liquid II to TMEDA is 1: 0.002-0.02.
In one embodiment according to the invention, in step 3), the dispersion II is reacted with (NH)4)2S2O8The mass ratio of (A) to (B) is 1: 0.007-0.008.
The invention also provides the iron-based nanoenzyme hydrogel prepared by the preparation method.
The invention also provides application of the iron-based nanoenzyme hydrogel in preparation of medical devices, in-vivo implants or anti-bacterial infection dressings; preferably, the dressing against bacterial infection is an anti-infective gel.
In one embodiment according to the invention, the anti-infective dressing is for the treatment of an infection by a pathogenic bacterium selected from any one or more of staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA), pseudomonas aeruginosa, escherichia coli, proteus, shigella dysenteriae and salmonella typhi.
The invention has the beneficial effects that:
the hydrogel prepared by the invention can effectively capture bacteria through the macroporous structure and electropositivity of the hydrogel, and catalyzes trace hydrogen peroxide to generate hydroxyl free radicals which have strong destructive effect on cell membranes or biological membranes through the peroxidase-like activity of the iron-based nanoenzyme, so that bacteria are killed, and the generation of drug-resistant bacteria is effectively avoided. Meanwhile, the iron-based nano enzyme hydrogel can remove dead bacteria, so that the effects of no inflammation and rapid wound healing are achieved.
The hydrogel prepared by the in-situ free radical polymerization method coats the iron-based nanoenzyme to obtain a novel nanoenzyme hydrogel. In vitro antibacterial activity detection results show that the iron-based nano enzyme hydrogel prepared by the invention can well inhibit and kill gram-positive bacteria and gram-negative bacteria, and more importantly, the result of mouse experimental study shows that the hydrogel can heal wounds infected by bacteria more quickly without inflammation and cytotoxicity. Therefore, the iron-based nanoenzyme hydrogel synthesized by the invention is expected to be applied to clinical antibiosis, so that more efficient and safe methods are provided for clinical antimicrobial treatment, and the method is helpful for solving clinical treatment problems of increasingly severe drug resistance, stubborn pathogenic microorganisms, newly-appeared harmful microorganisms and the like. The iron-based nano enzyme hydrogel prepared by the invention is simple and convenient to operate and easy to produce in batches.
Drawings
FIG. 1 is a scanning electron microscope of the iron-based nanoenzyme, hydrogel and iron-based nanoenzyme hydrogel prepared in example 1 of the present invention.
FIG. 2 is an X-ray diffraction spectrum and an infrared spectrum of the iron-based nanoenzyme hydrogel prepared in example 1 of the present invention.
FIG. 3 is Zeta potential diagram of the iron-based nanoenzyme hydrogel prepared in example 1 of the present invention.
FIG. 4 is a graph showing the inhibitory effect of the iron-based nanoenzyme hydrogel prepared in example 1 of the present invention on Staphylococcus aureus and Escherichia coli.
FIG. 5 shows the healing of the wound infected by Staphylococcus aureus in mice treated with the iron-based nanoenzyme hydrogel prepared in example 1 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and specific embodiments. Examples of which are illustrated in the accompanying drawings. It should be understood that the specific examples described in the following description of the embodiments of the present invention are merely illustrative of specific embodiments of the present invention and are intended to be used for the purpose of explanation, not limitation, of the invention.
Example 1 preparation of iron-based nanoenzyme hydrogel
Dissolving 60mg of NIPAAM, 20mg of AAM, 20mg of DMPA and 6mg of MBA in 1mL of deionized water, and carrying out ultrasonic treatment for 10min until complete dissolution to obtain a solution I; dispersing the prepared iron phosphate nano enzyme in 0.1mL of deionized water, adding the solution into the solution I, and continuing to perform ultrasonic treatment until the solution is uniformly dispersed to obtain a dispersion liquid II; 20 μ L of TMEDA and 20 μ L of 10% (NH)4)2S2O8Adding the mixture into the dispersion liquid II, and carrying out ultrasonic treatment until the mixture is completely uniform to obtain a mixed solution III; and pouring the mixed solution III into a hydrogel material forming die, and standing at room temperature for 3 hours. After the hydrogel is formed, the hydrogel is transferred into ultrapure water for soaking, and the residual TMEDA and (NH) are washed off4)2S2O8Then soaking the hydrogel in tert-butyl alcohol to replace the water in the hydrogel. And finally, extracting the hydrogel in liquid nitrogen for two or three times, and freeze-drying the hydrogel in a freeze dryer to obtain 115.55mg of the iron-based nano enzyme hydrogel with the macroporous structure, wherein the yield is 99.6%, and the purity is about 98.8%.
Example 2 characterization of iron-based nanoenzyme hydrogels
(1) Electron microscopy analysis (SEM)
The hydrogel, the iron-based nanoenzyme and the iron-based nanoenzyme hydrogel prepared in the example were subjected to morphological observation and analysis by using a scanning electron microscope (JSM7500F), and the results are shown in fig. 1:
in FIG. 1, a is a scanning electron micrograph of the morphology of the hydrogel, which shows that the porous network structure of the hydrogel has a pore size of about 2-4 μm. In fig. 1, b is a scanning electron microscope photograph of the morphology of the iron-based nanoenzyme, and the spheroidal structure of the iron-based nanoenzyme can be observed. In fig. 1, c is a scanning electron microscope photograph of the shape of the iron-based nanoenzyme hydrogel, which shows that the iron-based nanoenzyme is successfully coated in the hydrogel.
(2) XRD and FT-IR analysis
The iron-based nanoenzyme hydrogel prepared in this example was characterized for crystal structure and functional groups using an X-ray diffractometer (ULTIMALV) and a fourier infrared spectrometer (Nicolet iS50), and the results are shown in fig. 2:
diffraction peaks in an XRD (X-ray diffraction) pattern of the iron-based nanoenzyme in the figure 2 a can be seen, and the prepared iron-based nanoenzyme is in an amorphous crystal form. In fig. 2 b, it can be seen that the iron-based nanoenzyme is 1036cm-1And 3445cm-1Has obvious characteristic peaks corresponding to Fe-O-P and H of the iron-based nanoenzyme2Stretching and contracting-OH in O; hydrogel concentration at 3447cm-1And 1652cm-1Has obvious characteristic peaks corresponding to hydrogel amido bonds (-CO-NH-) and-NH2The stretching vibration of (2). The iron-based nano enzyme hydrogel is 2970cm-1And 1650cm-1Has obvious characteristic peaks corresponding to the stretching vibration of C-H and amido bonds (-CO-NH-) in the iron-based nano enzyme hydrogel.
(3) Zeta potential analysis
The potential analyzer was used to perform potential analysis on the iron-based nanoenzyme hydrogel prepared in example 1, and the result is shown in fig. 3:
FIG. 3 is Zeta potential diagram of iron-based nanoenzyme, hydrogel and iron-based nanoenzyme hydrogel, showing that hydrogel is positively charged at about +39mV, iron-based nanoenzyme is negatively charged at about-33 mV, and iron-based nanoenzyme macroporous hydrogel is positively charged at about +20 mV. Therefore, the iron-based nanoenzyme hydrogel prepared in example 1 can effectively capture negatively charged bacteria.
Example 3 antibacterial experiment of iron-based nanoenzyme hydrogel
(1) Preparing a first-level seed solution: respectively taking 100 mu L of each gram positive bacterium staphylococcus aureus (S.aureus ATCC 6538) and gram negative bacterium escherichia coli (E.coli ATCC 8739) frozen in a laboratory, placing in 100mL of LB broth (Haibo organism) liquid culture medium, carrying out constant temperature shaking culture for 14h (37 ℃, 120rpm) to obtain a first-stage seed solution, wherein the strains are purchased from Shanghai Lu micro-technology Limited company.
(2) Preparing a secondary seed solution: transfer 100. mu.L of each of the primary seed solutions obtained in (1) to a new 100mL LB liquid medium according to the absorbance (OD) of the bacterial solution at 600nm600) The bacterial concentration is preferably measured in the range of 0.6 to 0.8. Obtaining a secondary seed liquid.
(3) Preparing an antibacterial mother solution: weighing (sucking) a proper amount of hydrogel, iron-based nanoenzyme and H2O2Iron-based nanoenzyme + H2O2Iron-based nanoenzyme hydrogel and iron-based nanoenzyme hydrogel + H2O2Adding the mixture into the secondary seed liquid obtained in the step (2) to ensure that the concentration of the material is 12 mu g/mL and H is2O2The concentration is 0.2mmol/L, and the bacteria is co-cultured, and the constant temperature shaking culture is carried out for 30min (37 ℃, 120rpm), thus obtaining the antibacterial mother liquor. And a blank control was set.
(4) Counting the survival rate of bacteria by a plate colony counting method: the bacterial suspension added with different material components in the step (3) is subjected to gradient dilution, and the bacterial suspension containing the escherichia coli is subjected to gradient dilution with five concentrations of 10-3To 10-7And the bacterial suspension containing staphylococcus aureus is diluted by six concentrations in a gradient way, and the concentration is 10-3To 10-8And coating 100 mu L of diluent on a nutrient agar plate, arranging three groups of parallel concentration gradients, inverting the plate into a constant-temperature incubator at 37 ℃ for constant-temperature culture for 12h, calculating plate colonies and counting the survival rate of bacteria.
Example 4 in vitro Effect of iron-based nanoenzyme hydrogels on survival rates of Staphylococcus aureus and Escherichia coli
This example is PBS, hydrogel, iron phosphate nanoenzyme, H2O2Iron phosphate nanoenzyme + H2O2Iron phosphate nanoenzyme hydrogel and method for producing the sameAnd ferric phosphate nano enzyme hydrogel + H2O2Inhibitory effect on Staphylococcus aureus (S.aureus ATCC 6538) and Escherichia coli (E.coli ATCC 25922), respectively. The experimental procedure and procedure were the same as in example 3, and the inhibition ratio was (B-a)/B × 100%, where B is the number of colonies on agar plates with PBS solution as negative control, and a is the number of bacterial colonies on LB plates containing various nanomaterials. FIG. 4 shows the inhibitory effect of each material on Staphylococcus aureus and Escherichia coli, and compared with the blank control group, each material has a certain inhibitory effect, and compared with the other materials in this example, the iron phosphate nanoenzyme hydrogel + H prepared in this example2O2Has the strongest bacteriostatic effect on staphylococcus aureus and escherichia coli. In addition, the iron phosphate nanoenzyme hydrogel + H prepared in this example was analyzed by bacterial survival rate statistics2O2The bacteriostasis rates to staphylococcus aureus and escherichia coli are respectively 98.2 percent and 96.1 percent.
Example 5 wound treatment and healing assay in mice
In this example, PBS, hydrogel, iron phosphate nanoenzyme, H were used2O2Iron phosphate nanoenzyme + H2O2Iron phosphate nano enzyme hydrogel and iron phosphate nano enzyme hydrogel + H2O2Wound healing of s.aureus infections treated separately. In the wound model and healing process of the living mouse, the hydrogel, the ferric phosphate nanoenzyme hydrogel and the H are prepared by using sterile water2O2Four kinds of antibacterial liquid. Male Kunming mice (4 weeks old, 18-23g), purchased from Jinanpunyue laboratory animal breeders, Inc., were randomly divided into 6 groups: PBS group, hydrogel, ferric phosphate nano-enzyme and H2O2Iron phosphate nanoenzyme + H2O2Iron phosphate nano enzyme hydrogel and iron phosphate nano enzyme hydrogel + H2O26 per group. A wound surface of 6mm in diameter was obtained by surgery on the back of the mice after anesthesia. Infection of the wound with a suspension of Staphylococcus aureus (1X 10)5CFU mL-1). After 12h, recording the weight and taking a picture to record the wound healing condition. Dropping 5 μ L of antibacterial liquid to the correspondingThe wound surface was assembled, bandaged with sterile cotton cloth, the antibacterial liquid was given once every 24 hours and the sterile cotton cloth was replaced, photographs of the wound were taken after 2 days and 5 days, respectively, and mouse wound tissue was taken for analysis.
As shown in FIG. 5, the ferric phosphate nanoenzyme + H provided by the invention2O2And ferric phosphate nano enzyme hydrogel + H2O2The bacteriostatic effect of the composition is better than that of the white control group in the embodiment; compared with other materials in each group, the ferric phosphate nano enzyme + H2O2And ferric phosphate nano enzyme hydrogel + H2O2Wound healing of treated staphylococcus aureus infection was better, and the iron phosphate nanoenzyme hydrogel + H prepared in this example was used for wound healing2O2The wounds with the treated S.aureus infection healed best, almost well within 3 days.
The iron-based nanoenzyme hydrogel prepared by the in-situ free radical polymerization method has peroxidase-like activity in H2O2In the presence of the active hydroxyl radicals, the active hydroxyl radicals can destroy the cell membrane or cell wall of bacteria. The macroporosity and electropositivity of the material can capture and confine bacteria within the destruction range of hydroxyl radicals, immobilize the hydroxyl radicals, and ultimately kill the bacteria. In vitro antibacterial activity detection results show that the iron-based nano enzyme hydrogel prepared by the invention can well inhibit and kill gram-positive bacteria and gram-negative bacteria, and more importantly, the result of mouse experimental study shows that the hydrogel can heal wounds infected by bacteria more quickly without inflammation and cytotoxicity. Therefore, the iron-based nanoenzyme hydrogel synthesized by the invention is expected to be applied to clinical antibiosis, so that more efficient and safe methods are provided for clinical antimicrobial treatment, and the method is helpful for solving clinical treatment problems of increasingly severe drug resistance, stubborn pathogenic microorganisms, newly-appeared harmful microorganisms and the like. The iron-based nanoenzyme macroporous hydrogel prepared by the method is simple and convenient to operate and easy to produce in batches.
Finally, the above embodiments are only used to illustrate the technical solutions of the present invention, and do not limit the content of the present invention. Although the present invention has been described in considerable detail with reference to the embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of an iron-based nanoenzyme hydrogel is characterized by comprising the following steps:
1) dissolving N-isopropenylacrylamide (NIPAAM), acrylamide (AAM), N- (3-Dimethylaminopropyl) Methacrylamide (DMPA) and N, N-Methylene Bisacrylamide (MBA) in deionized water to obtain a mixed solution I;
2) dropwise adding a proper amount of iron-based nano enzyme dispersion liquid into the mixed solution I, and uniformly dispersing to obtain a dispersion liquid II;
3) proper amount of tetramethyl ethylenediamine (TMEDA) and ammonium persulfate [ (NH)4)2S2O8]Respectively adding the aqueous solution into the dispersion liquid II, uniformly mixing to obtain a mixed solution III, placing the mixed solution III into a hydrogel forming die, and standing at room temperature; preferably standing for 3 hours;
4) washing off residual TMEDA and (NH) after hydrogel forming4)2S2O8Then soaking the hydrogel in tert-butyl alcohol to replace the water in the hydrogel;
5) extracting the hydrogel treated in the step 4) in liquid nitrogen for several times, and freeze-drying to obtain the iron-based nano enzyme hydrogel.
2. The method according to claim 1, wherein in step 1), the mass ratio of NIPAAM, AAM, DMPA, MBA to deionized water is 8-10: 2.8-3:.22.8-3.2:1:150-155.
3. The method of claim 1, wherein in step 2), the iron-based oxide in the iron-based nanoenzyme is selected from Fe2O3、Fe3O4、FeMnO3、Fe2(MoO4)3FeOOH or FePO4One kind of (1).
4. The preparation method according to claim 1, wherein in the step 2), the mass fraction of the iron-based nanoenzyme in the iron-based nanoenzyme dispersion is 0.1% to 2.0%.
5. The preparation method of claim 1, wherein in the step 2), the acceleration of the iron-based nanoenzyme dispersed droplet is 10 to 100 μ L/s.
6. The method according to claim 1, wherein in step 3), (NH)4)2S2O8The mass fraction of the aqueous solution is 1-10%.
7. The method according to claim 1, wherein in step 3), (NH)4)2S2O8The volume ratio of the aqueous solution to the TMEDA is 1: 0.5-3, preferably 1: 0.8-1.5.
8. The preparation method according to claim 1, wherein in the step 3), the volume ratio of the dispersion liquid II to the TMEDA is 1: 0.002-0.02; preferably, the dispersion II is mixed with (NH)4)2S2O8The mass ratio of (A) to (B) is 1: 0.007-0.008.
9. The iron-based nanoenzyme hydrogel prepared by the preparation method according to any one of claims 1 to 8.
10. Use of the iron-based nanoenzyme hydrogel of claim 9 in the manufacture of a medical device, an in vivo implant, or an anti-bacterial infection dressing; preferably an anti-bacterial infection gel;
preferably, the anti-infective dressing is a dressing for treating infection by pathogenic bacteria selected from any one or more of staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA), pseudomonas aeruginosa, escherichia coli, proteus, shigella dysenteriae and salmonella typhi.
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