CN113252648B

CN113252648B - Quick detection swab for living microorganism ATP and use method thereof

Info

Publication number: CN113252648B
Application number: CN202110430246.2A
Authority: CN
Inventors: 孙华云
Original assignee: Shandong Vocational College of Industry
Current assignee: Shandong Vocational College of Industry
Priority date: 2021-04-21
Filing date: 2021-04-21
Publication date: 2022-11-22
Anticipated expiration: 2041-04-21
Also published as: CN113252648A

Abstract

The invention relates to a rapid detection swab for living microorganism ATP and a using method thereof, belonging to the field of microorganism detection. Including the sleeve pipe, the upper portion opening is sealed to the sleeve pipe bottom, sets up the rotary drum in the sleeve pipe, and the up end of rotary drum sets up the counter bore, and the counter bore is the torsion pattern, counter bore fit-in torsion bar, and the torsion bar is the torsion pattern, and the torsion bar passes through torsional surface fit with the counter bore, the solid fixed ring shape gasbag in the rotary drum upper end, annular gasbag upper end fixed connection guide cylinder, guide cylinder and sleeve cooperation, the axial sets up a plurality of capillary holes on the rotary drum wall. The reaction between the microbial lysate and the enzymatic reaction solution is more sufficient, the detection result is more accurate, the use is simple and convenient, and the enzymatic reaction solution and the water sample can be thoroughly emptied, so that the detection result has higher reference value.

Description

Quick detection swab for living microorganism ATP and use method thereof

Technical Field

The invention relates to a rapid detection swab for living microorganism ATP and a using method thereof, belonging to the field of microorganism detection.

Background

ATP content in living microorganisms is relatively constant. The swab determines the ATP content by detecting the luminescence value, thereby determining the content of living microorganisms in the sample. The swab has two structures, the lower part of the connector of the surface swab is connected with a cotton swab, and the cotton swab contains bacterial lysate for lysing microorganisms such as bacteria; the lower part of the connector of the water sample collector is connected with a suction pipe type water sample collector, and the sleeve is internally provided with bacterial lysate of microorganisms such as lytic bacteria and the like. The swab head contains an enzyme reaction solution which can react with ATP to emit light. The sample on the surface of the object is collected by a cotton swab or the liquid sample is collected by a water sample collector, the sample reacts with the bacterial lysate to release ATP in the microorganism, and then the ATP is mixed with the enzyme reaction liquid to generate reaction luminescence, and the luminescence value can be read by an instrument.

Microbial detection is usually carried out in outdoor places, and staff usually need carry two kinds of swabs, and it will lead to detecting unable going on to omit any kind of swab, and is very inconvenient. The enzyme reaction liquid is required to be fully mixed with the cracked microorganisms to enable all ATP and the enzyme reaction liquid to react and emit light, and the existing swab is usually simply mixed in a shaking mode, so that the mixing is insufficient, and the detection accuracy is influenced. The swab head contains the enzymatic reaction liquid, and when the swab head is used, a valve in the swab head needs to be folded back and forth to break the valve, and then the enzymatic reaction liquid is extruded out. In water sample collection, a quantitative water sample needs to be absorbed to perform a mixing reaction with a quantitative enzyme reaction solution, but when the water sample is collected, the amount of the absorbed water sample is usually determined through visual inspection by an operator, and the method is not accurate enough, so that the detection result has no high reference value.

Disclosure of Invention

According to the defects in the prior art, the technical problems to be solved by the invention are as follows: the rapid detection swab for living microorganism ATP and the use method thereof are provided, the reaction of the microorganism lysate and the enzymatic reaction solution is more sufficient, the detection result is more accurate, the use is simple and convenient, and the enzymatic reaction solution and a water sample can be thoroughly emptied, so that the detection result has higher reference value.

The invention relates to a rapid detection swab for living microorganism ATP, which comprises a sleeve, wherein the bottom of the sleeve is closed, the upper part of the sleeve is opened, a rotary drum is arranged in the sleeve, the upper end surface of the rotary drum is provided with a counter bore, the counter bore is in a torsion mode, a torsion bar is matched in the counter bore and is in a torsion mode, the torsion bar is matched with the counter bore through the torsion surface, the upper end of the rotary drum is fixedly provided with a ring-shaped air bag, the upper end of the ring-shaped air bag is fixedly connected with a guide cylinder, the guide cylinder is matched with a sleeve, the wall of the rotary drum is axially provided with a plurality of capillary holes, the bottom of the rotary drum is provided with a pit, the upper ends of the capillary holes are communicated with the ring-shaped air bag, the lower ends of the capillary holes are communicated with the pit, a pipette is coaxially fixed at the bottom of the rotary drum and is communicated with the pit, and enzyme reaction liquid is stored in the ring-shaped air bag.

The working principle and the process are as follows:

at ordinary times, the swab is stored in a refrigerator, and the long capillary greatly reduces the contact chance of the enzyme reaction liquid and the air, thereby preventing deterioration. When the device is used, the sleeve is held by four fingers, the guide cylinder is pressed by a thumb, the annular air bag is compressed by the guide cylinder, enzymatic reaction liquid in the annular air bag enters the sleeve along capillary holes, then the guide cylinder is pressed by the thumb, the sleeve is pulled down, the lower end of the pipette is stretched into a water sample to absorb the water sample, the thumb is loosened, the annular air bag bounces under the action of self elasticity to reset, the water sample is sucked into the pipette, the sleeve is sleeved, the guide cylinder is pressed again, the water sample in the pipette is pressed into the sleeve, bacterial lysate in the water sample reacts with the enzymatic reaction liquid to emit light, and then the swab is immediately placed into a detection instrument to be detected. Compared with the traditional swab, the method has the advantages that the operation is simpler, the enzyme reaction solution or the water sample can be thoroughly emptied by pressing for multiple times, and the detection accuracy is ensured.

The inner wall of the sleeve is provided with a stop block, and the distance between the stop blocks is larger than the diameter of the pipette and smaller than the diameter of the rotary drum. The rotary drum is blocked by the stop block, and a limiting effect is achieved.

A plurality of strings are arranged in the annular air bag, the two ends of each string are respectively and fixedly connected with the upper end and the lower end of the annular air bag, and when the strings are straightened, the strings are parallel to the axis of the annular air bag. When the annular air bag compresses, the string can not obstruct the compression of the annular air bag, when the string is straightened, the maximum expansion height of the annular air bag is fixed, and when a water sample is absorbed through negative pressure, the volume of the absorbed water sample is fixed, so that the detection result is more accurate by the quantitative reaction mode.

The upper end of the guide cylinder is fixed with a push disc, and the diameter of the push disc is larger than that of the guide cylinder. When the annular air bag bounces, the thumb nail is used for pushing the push disc upwards, so that the annular air bag can be expanded as soon as possible, liquid suction is rapid, and efficiency is improved.

The stirring ring is cooperated with the bottom of the sleeve, a plurality of notches are evenly distributed on the inner wall of the stirring ring, the inner diameter of the stirring ring is larger than the outer diameter of the liquid suction pipe, the lower part of the liquid suction pipe is fixed with a protrusion, the protrusion is matched with the notches, a plurality of stirring rods are fixed on the lower part of the stirring ring, and thin rods are vertically fixed at the lower ends of the stirring rods. Because the torsion bar is matched with the counter bore through the twisted surface, when the guide cylinder is pressed down or lifted up, the rotary drum rotates in the positive direction or the positive direction, the rotary drum drives the liquid suction pipe to rotate, the liquid suction pipe drives the stirring ring to rotate, and the stirring ring drives the slender rod to stir the mixed liquid of the water sample and the enzyme reaction liquid, so that the mixed liquid is fully mixed and reacted, and the detection result is ensured to be accurate and reliable.

The bulge is arc-shaped. The arc can play the guide effect for the arch can smoothly cooperate with the breach.

A limiting block is fixed on the push disc, and an arc-shaped groove is formed in the limiting block. When the thumb presses the guide cylinder, the thumb is clamped in the groove, so that the rotation of the guide cylinder can be limited, and the torsion bar is prevented from rotating and the rotary drum rotates.

Including the extension rod, the extension rod external diameter is unanimous with pipette internal diameter, and the fixed cotton ball of extension rod lower extreme sets up ventilative groove in the last circumference of extension rod. When the surface microorganism detection is required, the annular air bag is extruded to extrude the enzyme reaction liquid into the sleeve from the ventilating groove, then the extension rod is inserted into the lower end of the pipette, the surface of the object to be detected is wiped by the cotton ball to sample, then the pipette is inserted into the sleeve, the cotton ball is immersed into the enzyme reaction liquid, the air bag is extruded for many times, the fine rod is rotated positively and negatively to stir the enzyme reaction liquid, so that the microorganism lysate on the cotton ball is fully reacted with the enzyme reaction liquid, and the detection accuracy is ensured.

A limit frame is stuck on the guide cylinder, the limit frame is in a square frame shape, the inner side length of the limit frame is 10cm, and the frame width is not less than 0.5cm. The limiting frame is torn off and stuck on the surface of the object to be detected, and the cotton ball is wiped and sampled in the limiting frame. The common method is to sample a 10cm multiplied by 10cm area during sampling, the limit frame can enable the sampling area to be more accurate, compared with the traditional visual sampling mode, the frame width is large enough, and the cotton ball is prevented from being wiped to the area outside the frame.

Compared with the prior art, the invention has the beneficial effects that:

when the swab is used, the sleeve is held by four fingers, the guide cylinder is pressed by a thumb, the annular air bag is compressed by the guide cylinder, the enzyme reaction liquid in the annular air bag enters the sleeve along capillary pores, then the guide cylinder is pressed by the thumb, the sleeve is pulled down, the lower end of the pipette is stretched into a water sample to suck the water sample, the thumb is loosened, the annular air bag bounces under the action of the elasticity of the annular air bag to reset, the water sample is sucked into the pipette, then the sleeve is sleeved, the guide cylinder is pressed again, the water sample in the pipette is pressed into the sleeve, the bacterial lysate in the water sample reacts with the enzyme reaction liquid to emit light, and then the swab is immediately placed into a detection instrument to be detected. Compared with the traditional swab, the method is simpler to operate, and can thoroughly empty the enzymatic reaction solution or the water sample in a multi-pressing mode, so that the detection accuracy is ensured. The surface detection and the water sample detection can be realized simultaneously, the production cost is reduced, and the convenience of operators is improved.

Drawings

FIG. 1 is a schematic view of an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of embodiment A shown in FIG. 1;

FIG. 3 is an enlarged view of a portion of embodiment B shown in FIG. 1;

FIG. 4 is a perspective view of the embodiment of FIG. 2 showing the engagement of the torsion bar with the drum;

FIG. 5 is a schematic cross-sectional view of embodiment C-C of FIG. 3;

fig. 6 is a schematic diagram of a limiting frame according to an embodiment of the invention.

In the figure: 1. a sleeve; 2. a pipette; 3. a limiting block; 4. pushing a disc; 5. a guide cylinder; 6. an annular air bag; 7. a string; 8. a torsion bar; 9. capillary pores; 10. a drum; 11. a counter bore; 12. a pit; 13. a stopper; 14. a protrusion; 15. a stirring ring; 16. a notch; 17. a stirring rod; 18. a ventilation groove; 19. an extension pole; 20. a cotton ball; 21. a thin rod; 22. and a limiting frame.

Detailed Description

In the present invention, unless otherwise explicitly specified and defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be understood that the terms "central," "lateral," "longitudinal," "front," "rear," "left," "right," "upper," "lower," "vertical," "horizontal," "top," "bottom," "inner," "outer," "side," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the patent and for simplicity in description, and do not indicate or imply that the devices or components shown must be in a particular orientation, constructed and operated in a particular orientation, and are not to be considered limiting of the scope of the present invention.

In the present invention, unless otherwise explicitly stated or defined, the terms "mounted," "connected," "fixed," "fitted and fixed," and the like are to be understood broadly, and for example, may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium; there may be communication between the interiors of the two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

The present invention will be described more fully hereinafter with reference to the accompanying drawings of embodiments of the invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, by which the invention may be more fully understood; rather, these implementation examples are provided by way of example so that this disclosure will convey the scope of the invention to those skilled in the art. Further, the figures are merely schematic and not drawn to scale, and like numerals refer to like or similar elements or components throughout.

Embodiments of the invention are further described below with reference to the accompanying drawings:

as shown in fig. 1-6, the swab for ATP rapid detection of living microorganisms according to the present invention includes a casing 1, a closed upper opening is formed at the bottom of the casing 1, a rotating drum 10 is disposed in the casing 1, a counter bore 11 is disposed on an upper end surface of the rotating drum 10, the counter bore 11 is in a torsion pattern, a torsion bar 8 is fitted in the counter bore 11, the torsion bar 8 is in a torsion pattern, the torsion bar 8 is fitted with the counter bore 11 through a twisted surface, a ring-shaped air bag 6 is fixed at an upper end of the rotating drum 10, a guide cylinder 5 is fixedly connected to an upper end of the ring-shaped air bag 6, the guide cylinder 5 is fitted with the casing, a plurality of capillary holes 9 are axially disposed on a wall of the rotating drum 10, a pit 12 is disposed at the bottom of the rotating drum 10, an upper end of the capillary hole 9 is communicated with the ring-shaped air bag 6, a pipette 2 is coaxially fixed at the bottom of the rotating drum 10, the pipette 2 is communicated with the pit 12, and an enzyme reaction solution is stored in the ring-shaped air bag 6.

The working principle and the process are as follows:

the swab is stored in a refrigerator at ordinary times, and the long capillary holes 9 greatly reduce the contact chance between the enzyme reaction solution and the air and prevent deterioration. When the device is used, the sleeve 1 is held by four fingers, the guide cylinder 5 is pressed by a thumb, the annular air bag 6 is compressed by the guide cylinder 5, enzyme reaction liquid in the annular air bag 6 enters the sleeve 1 along the capillary hole 9, then the guide cylinder 5 is pressed by the thumb, the sleeve 1 is pulled down, the lower end of the pipette 2 is stretched into a water sample to suck the water sample, the thumb is loosened, the annular air bag 6 bounces and resets under the self elasticity, the water sample is sucked into the pipette 2, then the sleeve 1 is sleeved, the guide cylinder 5 is pressed again, the water sample in the pipette 2 is pressed into the sleeve 1, bacteria lysate in the water sample reacts with the enzyme reaction liquid to emit light, and then the swab is immediately placed into a detection instrument to be detected. Compared with the traditional swab, the method is simpler to operate, and can thoroughly empty the enzymatic reaction solution or the water sample in a multi-pressing mode, so that the detection accuracy is ensured.

The inner wall of the sleeve 1 is provided with stoppers 13, and the interval between the stoppers 13 is larger than the diameter of the pipette 2 and smaller than the diameter of the drum 10. The stop 13 stops the drum 10 to limit the position.

A plurality of strings 7 are arranged in the annular air bag 6, two ends of each string 7 are respectively and fixedly connected with the upper end and the lower end of the annular air bag 6, and when the strings 7 are straightened, the strings 7 are parallel to the axis of the annular air bag 6. When annular gasbag 6 compresses, string 7 can not hinder annular gasbag 6's compression, and after string 7 straightened, annular gasbag 6's the biggest height of swelling just fixed, when absorbing the water sample through the negative pressure, the volume of the water sample of absorbing is just fixed so, and this kind of quantitative reaction's mode makes the testing result more accurate.

The upper end of the guide cylinder 5 is fixed with a push disc 4, and the diameter of the push disc 4 is larger than that of the guide cylinder 5. When the annular air bag 6 bounces, the thumb nail is used for pushing the push disc 4 upwards, so that the annular air bag 6 can be expanded as soon as possible, liquid suction is rapid, and efficiency is improved.

The bottom of sleeve pipe 1 cooperates stirring ring 15, and a plurality of breachs 16 of equipartition on the stirring ring 15 inner wall, stirring ring 15 internal diameter are greater than pipette 2 external diameters, 2 lower parts fixed protrusion 14 of pipette, and arch 14 and breach 16 cooperation, a plurality of puddlers 17 of 15 lower part fixed of stirring ring, puddler 17 lower extreme vertical fixation thin rod 21. Because the torsion bar 8 is matched with the counter bore 11 through the twisted surface, when the guide cylinder 5 is pressed down or lifted up, the rotary drum 10 rotates in the positive direction or the rotation direction, the rotary drum 10 drives the liquid suction pipe 2 to rotate, the liquid suction pipe 2 drives the stirring ring 15 to rotate, and the stirring ring 15 drives the thin rod 21 to stir the mixed liquid of the water sample and the enzyme reaction liquid, so that the mixed liquid is fully mixed and reacted, and the detection result is ensured to be accurate and reliable.

The projections 14 are arcuate. The arc shape may serve as a guide so that the protrusion 14 can smoothly fit into the notch 16.

A limiting block 3 is fixed on the push disc 4, and an arc-shaped groove is formed in the limiting block 3. When the thumb presses the guide cylinder 5, the thumb is clamped in the groove, so that the rotation of the guide cylinder 5 can be limited, and the torsion bar 8 is prevented from rotating and the rotary drum 10 rotates.

Comprises an extension rod 19, the outer diameter of the extension rod 19 is consistent with the inner diameter of the pipette 2, a cotton ball 20 is fixed at the lower end of the extension rod 19, and a ventilation groove 18 is circumferentially arranged on the extension rod 19. When the surface microorganism detection is required, the annular air bag 6 is extruded to extrude the enzyme reaction liquid from the ventilation groove 18 into the sleeve 1, then the extension rod 19 is inserted into the lower end of the pipette 2, the surface of the object to be detected is wiped through the cotton ball 20 for sampling, then the pipette 2 is inserted into the sleeve 1, the cotton ball 20 is immersed into the enzyme reaction liquid, the air bag is extruded for multiple times, and the fine rod 21 is rotated forward and backward to stir the enzyme reaction liquid, so that the microorganism lysate on the cotton ball 20 is fully reacted with the enzyme reaction liquid, and the detection accuracy is ensured.

The guide cylinder 5 is adhered with a limit frame 22, the limit frame 22 is in a square frame shape, the inner side length of the limit frame is 10cm, and the frame width is not less than 0.5cm. The limiting frame 22 is torn off and stuck on the surface of the object to be detected, and the cotton ball 20 is wiped and sampled in the limiting frame 22. In the sampling process, the area of 10cm × 10cm is sampled, the limit frame 22 can make the sampling area more accurate, compared with the traditional visual sampling mode, and meanwhile, the frame width is large enough to prevent the cotton ball 20 from being wiped to the area outside the frame.

These and other changes can be made to the present apparatus with reference to the above detailed description. While the above detailed description describes certain embodiments of the invention and describes the best mode contemplated, no matter how detailed the above appears in text, the system can be practiced in many ways. The details of the locally-based support device, although considerable variation in its implementation details is possible, are nevertheless contained within the device disclosed herein. As noted above, the use of particular technical terms in describing particular features or aspects of the present device does not imply that the terms are being redefined herein to be restricted to particular features, or system aspects associated with the terms. In general, the terms used in the following claims should not be construed to limit the system to the specific embodiments disclosed in the specification, unless the above detailed description section explicitly defines such terms. Accordingly, the actual scope of the system encompasses not only the disclosed embodiments, but also all equivalent ways of practicing or implementing the apparatus covered by the claims.

Claims

1. A living microorganism ATP rapid detection swab is characterized in that: the reaction solution reaction device comprises a sleeve (1), wherein an upper opening is sealed at the bottom of the sleeve (1), a rotary drum (10) is arranged in the sleeve (1), a counter bore (11) is formed in the upper end face of the rotary drum (10), the counter bore (11) is in a torsion mode, a torsion bar (8) is in the torsion mode, the torsion bar (8) is matched with the counter bore (11) through the torsion surface, a ring-shaped air bag (6) is fixed at the upper end of the rotary drum (10), a guide cylinder (5) is fixedly connected to the upper end of the ring-shaped air bag (6), the guide cylinder (5) is matched with the sleeve, a plurality of capillary holes (9) are axially formed in the wall of the rotary drum (10), a pit (12) is formed in the bottom of the rotary drum (10), the upper ends of the capillary holes (9) are communicated with the ring-shaped air bag (6), the lower ends of the capillary holes (9) are communicated with the pit (12), a liquid suction pipe (2) is coaxially fixed at the bottom of the rotary drum (10), and the liquid suction pipe (2) is communicated with the pit (12), and enzyme reaction solution is stored in the ring-shaped air bag (6);

including extension rod (19), extension rod (19) external diameter is unanimous with pipette (2) internal diameter, and extension rod (19) lower extreme fixed cotton ball (20), and extension rod (19) go up circumference and set up ventilative groove (18), and extension rod (19) insert pipette (2) lower extreme.

2. The swab for rapid ATP detection of a living organism according to claim 1, wherein: the inner wall of the sleeve (1) is provided with a stop block (13), and the distance between the stop blocks (13) is larger than the diameter of the pipette (2) and smaller than the diameter of the rotary drum (10).

3. The swab for rapid ATP detection of a living organism according to claim 1, wherein: a plurality of strings (7) are arranged in the annular air bag (6), the two ends of each string (7) are respectively and fixedly connected with the upper end and the lower end of the annular air bag (6), and when the strings (7) are straightened, the strings (7) are parallel to the axis of the annular air bag (6).

4. The swab for rapid ATP detection of a living organism according to claim 1, wherein: the upper end of the guide cylinder (5) is fixed with a push disc (4), and the diameter of the push disc (4) is larger than that of the guide cylinder (5).

5. The rapid ATP detection swab for living organisms according to claim 1, wherein: the bottom of the sleeve (1) is matched with the stirring ring (15), a plurality of notches (16) are uniformly distributed on the inner wall of the stirring ring (15), the inner diameter of the stirring ring (15) is larger than the outer diameter of the liquid suction pipe (2), the lower part of the liquid suction pipe (2) is fixed with a protrusion (14), the protrusion (14) is matched with the notches (16), a plurality of stirring rods (17) are fixed on the lower part of the stirring ring (15), and thin rods (21) are vertically fixed on the lower ends of the stirring rods (17).

6. The swab for rapid ATP detection of a living organism according to claim 5, wherein: the projection (14) is arc-shaped.

7. The swab for rapid ATP detection of a living organism according to claim 4, wherein: a limiting block (3) is fixed on the push disc (4), and an arc-shaped groove is formed in the limiting block (3).

8. The swab for rapid ATP detection of a living organism according to claim 5, wherein: a limiting frame (22) is adhered to the guide cylinder (5), the limiting frame (22) is square frame-shaped, the length of the inner side of the limiting frame is 10cm, and the frame width is not less than 0.5cm.

9. The method for using a swab for rapid detection of ATP in living organisms according to claim 8, wherein the swab comprises:

1. when the water sample microbial detection is carried out, when the device is used, the sleeve (1) is held by four fingers, the guide cylinder (5) is pressed down by a thumb, the annular air bag (6) is compressed by the guide cylinder (5), enzyme reaction liquid in the annular air bag (6) enters the sleeve (1) along the capillary hole (9), then the guide cylinder (5) is pressed down by the thumb, the sleeve (1) is pulled down, the lower end of the pipette (2) is stretched into a water sample to suck the water sample, the thumb is loosened, the annular air bag (6) bounces up and resets under the action of self elasticity, the water sample is sucked into the pipette (2), then the sleeve (1) is sleeved, the guide cylinder (5) is pressed again, the water sample in the pipette (2) is pressed into the sleeve (1), bacteria lysate in the water sample reacts with the enzyme reaction liquid to emit light, and then the swab is immediately placed into a detection instrument to be detected;

2. when surface microorganism detection is carried out, the annular air bag (6) is extruded to extrude enzyme reaction liquid into the sleeve (1) from the ventilating groove (18), then the extension rod (19) is inserted into the lower end of the pipette (2), the surface of an object to be detected is wiped through the cotton ball (20) to be sampled, then the pipette (2) is inserted into the sleeve (1), the cotton ball (20) is immersed into the enzyme reaction liquid, the annular air bag (6) is extruded for a plurality of times, the thin rod (21) is rotated positively and negatively to stir the enzyme reaction liquid, and therefore microorganism lysate on the cotton ball (20) is fully reacted with the enzyme reaction liquid.