CN113237819A - Flow cytometry detector - Google Patents
Flow cytometry detector Download PDFInfo
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- CN113237819A CN113237819A CN202110517278.6A CN202110517278A CN113237819A CN 113237819 A CN113237819 A CN 113237819A CN 202110517278 A CN202110517278 A CN 202110517278A CN 113237819 A CN113237819 A CN 113237819A
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- 238000000684 flow cytometry Methods 0.000 title abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 65
- 239000000463 material Substances 0.000 claims abstract description 50
- 239000007788 liquid Substances 0.000 claims description 73
- 238000004140 cleaning Methods 0.000 claims description 28
- 238000007789 sealing Methods 0.000 claims description 13
- 238000009434 installation Methods 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000007689 inspection Methods 0.000 claims description 8
- 230000003068 static effect Effects 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 10
- 230000010355 oscillation Effects 0.000 description 9
- 238000003756 stirring Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000005381 potential energy Methods 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a flow cytometry detector which comprises a material changing disc and a reversing mechanism, wherein a plurality of notches are uniformly formed in the edge of the material changing disc along the circumferential direction; a mounting base is arranged between the material changing plate and the reversing mechanism; the reversing mechanism comprises a rotary table, a material shifting mechanism and a motor, the rotary table is connected with the material changing disc through a rotating shaft, notches are uniformly formed in the edge of the rotary table along the circumferential direction, and the number of the notches is the same as that of the notches; the material shifting mechanism comprises an arc-shaped disc, a shifting rod and a roller, the bottom of the arc-shaped disc is connected with a motor, one end of the shifting rod is installed at the bottom of the arc-shaped disc, the other end of the shifting rod is connected with the roller, and the roller rotates the turntable through a notch in the rotating stroke of the shifting rod. The automatic conveying detection box is additionally provided with the material changing disc and the reversing mechanism which are in transmission connection with each other, and the automatic conveying detection box is realized by driving the material changing disc to rotate through the rotation of the reversing mechanism, so that the working efficiency is improved.
Description
Technical Field
The invention relates to the technical field of biological instruments, in particular to a flow cytometry detector.
Background
Along with the development of science and technology, the biological industry is developing more and more to high-end, for example, the prior sampling is performed by using an artificial magnifier for assay, a flow cytometer is used at present, the automatic assay of an electron microscope is realized, the technological content is greatly provided, the flow cytometer is a device for automatically analyzing and sorting cells, can quickly measure, store and display a series of important characteristic parameters of dispersed cells suspended in liquid in the aspects of biophysics and biochemistry, and can sort out specified cell subsets according to a preselected parameter range.
Flow cytometer generally can carry out diversified regulation among the prior art, makes things convenient for medical personnel to carry out multi-angle measurement, but has an important link before measuring, and the batching sample link promptly, in order to guarantee measurement accuracy, need wait to detect liquid and measure many times usually, gain the average value to reduce measuring error.
However, in the existing device, when the box to be detected is conveyed, manual conveying is generally adopted, the automation degree is low, and the working efficiency is slow.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the flow cytometry detector is provided, and the detection box is automatically conveyed, so that the working efficiency is improved.
In order to solve the technical problems, the invention adopts a technical scheme that: a flow cytometry detector comprises a material changing disc and a reversing mechanism, wherein a plurality of notches are uniformly formed in the edge of the material changing disc along the circumferential direction; a mounting base is arranged between the material changing plate and the reversing mechanism;
the reversing mechanism comprises a rotary disc, a material shifting mechanism and a motor, the rotary disc is connected with the material changing disc through a rotating shaft, notches are uniformly formed in the edge of the rotary disc along the circumferential direction, and the number of the notches is the same as that of the notches;
the material shifting mechanism comprises an arc-shaped disc, a shifting rod and a roller, the bottom of the arc-shaped disc is connected with a motor, one end of the shifting rod is installed at the bottom of the arc-shaped disc, the other end of the shifting rod is connected with the roller, and the roller rotates the turntable through a notch in the rotating stroke of the shifting rod.
The invention has the beneficial effects that: the material changing disc and the reversing mechanism which are in transmission connection with each other are additionally arranged, the arc-shaped disc is driven to rotate through the motor, the driving lever is driven to rotate, when the driving lever drives the roller to the notch position of the rotary disc, the rotary disc can be stirred to rotate in the opposite direction, the rotary shaft and the material changing disc are driven to synchronously rotate through the rotation of the rotary disc, and the material changing disc can enable the detection box to be conveyed on the installation base through the notch. Realize carrying the detection box automatically to improve work efficiency.
Drawings
FIG. 1 is a schematic structural diagram of a flow cytometer according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a mounting base of a flow cytometer in accordance with an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a material changing tray and a reversing mechanism of a flow cytometer according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a turntable structure of a flow cytometer according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a material stirring mechanism of a flow cytometer according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of an oscillation liquid injection mechanism of a flow cytometer according to an embodiment of the present invention;
FIG. 7 is a second gear mounting diagram of a flow cytometer in accordance with an embodiment of the present invention;
FIG. 8 is a schematic structural diagram of a feeding mechanism of a flow cytometer according to an embodiment of the present invention;
FIG. 9 is a schematic structural diagram of an inspection mechanism of a flow cytometer according to an embodiment of the present invention;
FIG. 10 is a schematic diagram of a cleaning mechanism of a flow cytometer according to an embodiment of the present invention;
description of reference numerals:
1. installing a base;
11. an annular groove; 12. a baffle plate; 13. a sloping plate;
2. changing the material plate; 21. a notch;
3. a reversing mechanism; 31. a turntable; 311. a notch; 32. a material poking mechanism; 321. an arc-shaped tray; 322. a deflector rod; 323. a drum; 33. a motor;
4. a storage cylinder;
5. a vibration liquid injection mechanism; 51. a first limit plate; 52. a first mounting plate; 53. a steering cylinder; 531. a chute; 54. a bump; 55. a liquid storage tank; 56. a second mounting plate; 57. a feeding mechanism; 571. sealing the barrel; 572. a catheter;
6. an inspection mechanism; 61. a second limiting plate; 62. a third mounting plate; 63. a stationary plate; 631. a limiting rod; 64. a cleaning mechanism; 641. a sliding sleeve; 642. a slide bar; 643. sliding the head; 644. cleaning the plate; 645. a spring; 65. a detection head; 66. a sliding disk; 661. an arc-shaped plate; 67. a connecting plate; 671. a bearing housing;
7. a first gear; 8. a rotating rod;
9. a spline shaft; 91. a groove; 92. a first piston plate; 93. a second piston plate; 94. a liquid outlet;
10. a second gear; 101. a spline housing.
Detailed Description
In order to explain technical contents, achieved objects, and effects of the present invention in detail, the following description is made with reference to the accompanying drawings in combination with the embodiments.
Referring to fig. 1, fig. 3, fig. 4 and fig. 5, an embodiment of the present invention provides a flow cytometer, including a material changing tray 2 and a reversing mechanism 3, wherein a plurality of notches 21 are uniformly formed along a circumferential direction on an edge of the material changing tray 2; a mounting base 1 is arranged between the material changing disc 2 and the reversing mechanism 3;
the reversing mechanism 3 comprises a rotary disc 31, a material shifting mechanism 32 and a motor 33, the rotary disc 31 is connected with the material changing disc 2 through a rotating shaft, notches 311 are uniformly formed in the edge of the rotary disc 31 along the circumferential direction, and the number of the notches 311 is the same as that of the notches 21;
the material shifting mechanism 32 comprises an arc-shaped disc 321, a shifting rod 322 and a roller 323, wherein the bottom of the arc-shaped disc 321 is connected with a motor 33, one end of the shifting rod 322 is installed at the bottom of the arc-shaped disc 321, the other end of the shifting rod 322 is connected with the roller 323, and the roller 323 rotates the turntable 31 through the notch 311 in the rotation stroke of the shifting rod 322.
From the above description, the beneficial effects of the present invention are: add material changing plate 2 and reversing mechanism 3 that the transmission is connected each other, and then drive the rotation of driving arc dish 321 through the drive of motor 33, and then drive the rotation of driving lever 322, when driving lever 322 drives cylinder 323 to the notch 311 position of carousel 31, can stir carousel 31 and carry out the opposite direction and rotate, drive pivot and material changing plate 2 synchronous rotation through the rotation of carousel 31, and then material changing plate 2 can make the detection box carry on installing base 1 through breach 21. Realize carrying the detection box automatically to improve work efficiency.
Further, a storage cylinder 4 is fixedly mounted on the mounting base 1, and a detection box is arranged in the storage cylinder 4 and is cylindrical.
Further, ring channel 11 has been seted up to installation base 1 upper surface, the groove width size of ring channel 11 with the external diameter of detection box is the same, ring channel 11 is provided with baffle 12 along the outer lane, baffle 12 is a not closed ring, baffle 12 is not closed department and is provided with the swash plate 13 that supplies to detect box roll-off installation base 1.
It can be known from the above description that the inside detection box of storage cylinder 4 can be if on installation base 1 because the action of gravity to be located the breach 21 of trading charging tray 2 inside, and then under the drive of motor 33, make the breach 21 of trading charging tray 2 promote the detection box and switch the station, can make the removal that detects the box more steady through ring channel 11, and protect the detection box in the transportation process through baffle 12, the detection box that finally accomplishes the test can pass through swash plate 13 roll-off installation base 1.
Further, a first gear 7 is installed above the material changing disc 2 through a rotating shaft, an oscillation liquid injection mechanism 5 is fixedly installed on the installation base 1, and the oscillation liquid injection mechanism 5 comprises a first limiting plate 51, a first installation plate 52, a steering cylinder 53, a convex block 54, a liquid storage tank 55, a second installation plate 56 and a feeding mechanism 57; the first limiting plate 51 is mounted on the upper surface of the mounting base 1, a first mounting plate 52 is arranged at one end, facing the annular groove 11, of the first limiting plate 51, a rotating rod 8 is slidably mounted on the first mounting plate 52, a steering cylinder 53 is arranged at the top of the rotating rod 8, and an inclined groove 531 is formed in the outer wall of the steering cylinder 53; the projection 54 is fixedly installed on the one end of the first limit plate 51, and a raised head of the projection 54 is slidably arranged in the inclined groove 531; a liquid storage tank 55 is arranged at the bottom of the rotating rod 8, a spline shaft 9 is arranged at the bottom of the liquid storage tank 55, and the inside of the spline shaft 9 is of a hollow structure and is communicated with the liquid storage tank 55; a groove 91 is formed in the upper end of the spline shaft 9, a second gear 10 meshed with the first gear 7 is clamped on the outer wall of the spline shaft 9, a spline sleeve 101 matched with the spline shaft 9 is formed in the middle of the second gear 10, a second mounting plate 56 is arranged at the position, opposite to the spline sleeve 101, of one end of the first limiting plate 51, two groups of second mounting plates 56 are arranged and are respectively located on the upper surface and the lower surface of the second gear 10, and the spline sleeve 101 is movably connected with the second mounting plate 56; the outer wall of the lower end of the spline shaft 9 is provided with a first piston plate 92, the bottom of the spline shaft 9 is provided with a second piston plate 93, the outer wall of the spline shaft 9 is provided with a liquid outlet 94, the liquid outlet 94 is positioned between the first piston plate 92 and the second piston plate 93, the first piston plate 92 and the second piston plate 93 stretch into the feeding mechanism 57, and the feeding mechanism 57 is arranged at the bottom of the second mounting plate 56.
Further, the feeding mechanism 57 comprises a sealing barrel 571 and a liquid guide pipe 572, the sealing barrel 571 is arranged at the bottom of the second mounting plate 56, the first piston plate 92 and the second piston plate 93 extend into the sealing barrel 571, and the liquid guide pipe 572 is arranged at the lower end of the sealing barrel 571.
It can be known from the above description, can drive first gear 7 synchronous rotation when reloading dish 2 rotates, and then drive second gear 10 and rotate, because spline housing 101 and integral key shaft 9 joint mutually, and then drive integral key shaft 9 and rotate, and then drive liquid storage pot 55 and rotate, wait to detect liquid to its inside and shake the formula stirring, prevent to wait that it detects the inside cell precipitation of liquid and influence detection precision, it rotates to drive bull stick 8 and steering tube 53 to move through liquid storage pot 55, because lug 54 has the guide effect to steering tube 53, lug 54's plush copper can slide in chute 531 inside when steering tube 53 rotates, and then drive steering tube 53 and reciprocate in vertical direction, and then drive bull stick 8, liquid storage pot 55, integral key shaft 9 reciprocates in step. Because the inside of integral key shaft 9 is hollow structure to with liquid storage pot 55 intercommunication, the liquid that awaits measuring of liquid storage pot 55 can flow into sealed bucket 571 through integral key shaft 9's liquid outlet 94 in, when integral key shaft 9 moves down, liquid outlet 94 can move down in step, owing to the sealed effect of first piston plate 92 and second piston plate 93 this moment, the liquid that awaits measuring can not overflow this moment, when liquid outlet 94 moved to catheter 572 position, the liquid that will await measuring is directed into the detection box below it through catheter 572, accomplish the automatic ration notes liquid function to the detection box.
Further, an inspection mechanism 6 is fixedly mounted on the mounting base 1, and the inspection mechanism 6 includes a second limiting plate 61, a third mounting plate 62, a stationary disc 63, a cleaning mechanism 64, a detection head 65, a sliding disc 66 and a connecting plate 67; the second limiting plate 61 is mounted on the upper surface of the mounting base 1, and a third mounting plate 62 is arranged at one end, facing the annular groove 11, of the second limiting plate 61; a static disc 63 is arranged at one end, away from the second limit plate 61, of the third mounting plate 62, two limit rods 631 are symmetrically arranged at the bottom of the static disc 63, and a cleaning mechanism 64 is connected to the bottom of each limit rod 631; the detection head 65 is electrically connected with a computer terminal, the outer wall of the detection head 65 is fixedly connected with a sliding disc 66, the sliding disc 66 is in sliding connection with two limiting rods 631, two arc-shaped plates 661 are arranged at the bottom of the sliding disc 66 corresponding to the cleaning mechanism 64, and the distance between the bottoms of the two arc-shaped plates 661 is smaller than that between the tops of the two arc-shaped plates 661; the top of the detection head 65 is connected with one end of a connecting plate 67, the other end of the connecting plate 67 is provided with a bearing sleeve 671 matched with the groove 91, the connecting plate 67 is connected with the spline shaft 9, and the bearing sleeve 671 and the groove 91 are mutually clamped.
Further, the cleaning mechanism 64 includes a sliding sleeve 641, a sliding rod 642, a sliding head 643, a cleaning plate 644 and a spring 645; the sliding sleeve 641 is connected with a limiting rod 631, the sliding sleeve 641 is slidably mounted on a sliding rod 642, one end of the sliding rod 642 is provided with a sliding head 643, the sliding head 643 is in contact with an arc-shaped plate 661, a spring 645 is arranged between the sliding head 643 and the sliding sleeve 641, and the other end of the sliding rod 642 is provided with a cleaning plate 644.
As can be seen from the above description, when the spline shaft 9 moves downwards, the groove 91 moves downwards at the same time, and further drives the bearing sleeve 671 to move downwards synchronously, and further drives the connecting plate 67 to move downwards, and further drives the detection head 65 to move downwards, so as to detect the liquid inside the detection box, and transmit data to the computer terminal, when the detection head 65 moves downwards, the sliding plate 66 and the arc plate 661 will be driven to slide downwards synchronously, since the height of the cleaning mechanism 64 is limited by the stationary plate 63 and the stop rod 631, the distance between the bottoms of the two arc plates 661 is smaller than the distance between the tops thereof, when the arc plate 661 moves downwards gradually, the sliding head 661 slides gradually from the bottom of the arc plate 661 to the top of the arc plate 661 642, at this time, due to the elastic potential energy of the spring 645, the sliding head 643 will be pushed to move in the direction away from the detection head 65, and further drive the sliding rod to move outwards, and further drive the two cleaning plates 644 to separate from the surface of the detection head 65 gradually, in a similar way, when detecting head 65 rebound, two cleaning board 644 can gradually with detect the laminating of head 65 outer wall this moment, will detect head 65 surface residual liquid stain and strike off then, prevent to influence down group's sample detection precision.
Example one
Referring to fig. 1, fig. 3, fig. 4 and fig. 5, a flow cytometer includes a material changing tray 2 and a reversing mechanism 3, wherein a plurality of notches 21 are uniformly formed on the edge of the material changing tray 2 along the circumferential direction; a mounting base 1 is arranged between the material changing disc 2 and the reversing mechanism 3; the reversing mechanism 3 comprises a rotary disc 31, a material shifting mechanism 32 and a motor 33, the rotary disc 31 is connected with the material changing disc 2 through a rotating shaft, notches 311 are uniformly formed in the edge of the rotary disc 31 along the circumferential direction, and the number of the notches 311 is the same as that of the notches 21; the material shifting mechanism 32 comprises an arc-shaped disc 321, a shifting rod 322 and a roller 323, wherein the bottom of the arc-shaped disc 321 is connected with a motor 33, one end of the shifting rod 322 is installed at the bottom of the arc-shaped disc 321, the other end of the shifting rod 322 is connected with the roller 323, and the roller 323 rotates the turntable 31 through the notch 311 in the rotation stroke of the shifting rod 322.
Referring to fig. 1 and 2, a storage cylinder 4 is fixedly mounted on the mounting base 1, and a detection box is disposed in the storage cylinder 4 and is cylindrical. The ring channel 11 has been seted up to installation base 1 upper surface, the groove width size of ring channel 11 with the external diameter of detection box is the same, ring channel 11 is provided with baffle 12 along the outer lane, baffle 12 is an unclosed ring, baffle 12 unclosed department is provided with the swash plate 13 that supplies detection box roll-off installation base 1.
Referring to fig. 1, 6, 7 and 8, a first gear 7 is mounted above the material changing tray 2 through a rotating shaft, an oscillation liquid injection mechanism 5 is fixedly mounted on the mounting base 1, and the oscillation liquid injection mechanism 5 includes a first limiting plate 51, a first mounting plate 52, a steering cylinder 53, a convex block 54, a liquid storage tank 55, a second mounting plate 56 and a feeding mechanism 57; the first limiting plate 51 is mounted on the upper surface of the mounting base 1, a first mounting plate 52 is arranged at one end, facing the annular groove 11, of the first limiting plate 51, a rotating rod 8 is slidably mounted on the first mounting plate 52, a steering cylinder 53 is arranged at the top of the rotating rod 8, and an inclined groove 531 is formed in the outer wall of the steering cylinder 53; the projection 54 is fixedly installed on the one end of the first limit plate 51, and a raised head of the projection 54 is slidably arranged in the inclined groove 531; a liquid storage tank 55 is arranged at the bottom of the rotating rod 8, a spline shaft 9 is arranged at the bottom of the liquid storage tank 55, and the inside of the spline shaft 9 is of a hollow structure and is communicated with the liquid storage tank 55; a groove 91 is formed in the upper end of the spline shaft 9, a second gear 10 meshed with the first gear 7 is clamped on the outer wall of the spline shaft 9, a spline sleeve 101 matched with the spline shaft 9 is formed in the middle of the second gear 10, a second mounting plate 56 is arranged at the position, opposite to the spline sleeve 101, of one end of the first limiting plate 51, two groups of second mounting plates 56 are arranged and are respectively located on the upper surface and the lower surface of the second gear 10, and the spline sleeve 101 is movably connected with the second mounting plate 56; the outer wall of the lower end of the spline shaft 9 is provided with a first piston plate 92, the bottom of the spline shaft 9 is provided with a second piston plate 93, the outer wall of the spline shaft 9 is provided with a liquid outlet 94, the liquid outlet 94 is positioned between the first piston plate 92 and the second piston plate 93, the first piston plate 92 and the second piston plate 93 stretch into the feeding mechanism 57, and the feeding mechanism 57 is arranged at the bottom of the second mounting plate 56. The feeding mechanism 57 comprises a sealing barrel 571 and a liquid guide pipe 572, the sealing barrel 571 is arranged at the bottom of the second mounting plate 56, the first piston plate 92 and the second piston plate 93 extend into the sealing barrel 571, and the liquid guide pipe 572 is arranged at the lower end of the sealing barrel 571.
Referring to fig. 1, 9 and 10, an inspection mechanism 6 is fixedly mounted on the mounting base 1, and the inspection mechanism 6 includes a second limiting plate 61, a third mounting plate 62, a stationary plate 63, a cleaning mechanism 64, a detection head 65, a sliding plate 66 and a connecting plate 67; the second limiting plate 61 is mounted on the upper surface of the mounting base 1, and a third mounting plate 62 is arranged at one end, facing the annular groove 11, of the second limiting plate 61; a static disc 63 is arranged at one end, away from the second limit plate 61, of the third mounting plate 62, two limit rods 631 are symmetrically arranged at the bottom of the static disc 63, and a cleaning mechanism 64 is connected to the bottom of each limit rod 631; the detection head 65 is electrically connected with a computer terminal, the outer wall of the detection head 65 is fixedly connected with a sliding disc 66, the sliding disc 66 is in sliding connection with two limiting rods 631, two arc-shaped plates 661 are arranged at the bottom of the sliding disc 66 corresponding to the cleaning mechanism 64, and the distance between the bottoms of the two arc-shaped plates 661 is smaller than that between the tops of the two arc-shaped plates 661; the top of the detection head 65 is connected with one end of a connecting plate 67, the other end of the connecting plate 67 is provided with a bearing sleeve 671 matched with the groove 91, the connecting plate 67 is connected with the spline shaft 9, and the bearing sleeve 671 and the groove 91 are mutually clamped. The cleaning mechanism 64 comprises a sliding sleeve 641, a sliding rod 642, a sliding head 643, a cleaning plate 644 and a spring 645; the sliding sleeve 641 is connected with a limiting rod 631, the sliding sleeve 641 is slidably mounted on a sliding rod 642, one end of the sliding rod 642 is provided with a sliding head 643, the sliding head 643 is in contact with an arc-shaped plate 661, a spring 645 is arranged between the sliding head 643 and the sliding sleeve 641, and the other end of the sliding rod 642 is provided with a cleaning plate 644.
The working principle is as follows: when the automatic feeding device is used, a plurality of groups of detection boxes are sequentially placed in the storage barrel 4, the motor 33 is started, the arc-shaped disc 321 is driven to rotate clockwise through the clockwise rotation of the motor 33, the driving lever 322 is driven to rotate clockwise, when the driving lever 322 drives the roller 323 to move to the position of the notch 311 of the turntable 31, the turntable 31 is driven to rotate anticlockwise, namely the motor 33 rotates for a circle, the turntable 31 rotates for a quarter period, the rotating shaft and the material changing disc 2 are driven to rotate anticlockwise synchronously through the anticlockwise rotation of the turntable 31, the detection boxes in the storage barrel 4 fall into the notch 21 under the action of gravity, so that the automatic feeding function of the detection boxes is realized, and the labor intensity of workers is reduced;
along with the counterclockwise rotation of the material changing disc 2 for a quarter period, the detection box is driven to slide above the annular groove 11 to the lower part of the oscillation liquid injection mechanism 5, when the material changing disc 2 rotates anticlockwise, the first gear 7 is driven to rotate synchronously, and then the second gear 10 is driven to rotate, because the spline housing 101 is clamped with the spline shaft 9, the spline shaft 9 is driven to rotate, the liquid storage tank 55 is driven to rotate, and meanwhile, the rotating rod 8 and the steering barrel 53 are driven to rotate through the rotation of the liquid storage tank 55, since the projection 54 has a guiding function for the steering column 53, the projection of the projection 54 slides inside the inclined groove 531 when the steering column 53 rotates, the steering cylinder 53 is driven to move up and down in the vertical direction, and the rotating rod 8, the liquid storage tank 55 and the spline shaft 9 are driven to synchronously move up and down to perform oscillation type stirring on the liquid to be detected in the liquid to be detected, so that the influence of cell precipitation in the liquid to be detected on the detection precision is prevented; because the inside of integral key shaft 9 is hollow structure to with liquid storage pot 55 intercommunication, the liquid that awaits measuring of liquid storage pot 55 can flow into sealed bucket 571 through integral key shaft 9's liquid outlet 94 in, when integral key shaft 9 moves down, liquid outlet 94 can move down in step, owing to the sealed effect of first piston plate 92 and second piston plate 93 this moment, the liquid that awaits measuring can not overflow this moment, when liquid outlet 94 moved to catheter 572 position, the liquid that will await measuring is directed into the detection box below it through catheter 572, accomplish the automatic ration notes liquid function to the detection box. Meanwhile, when the spline shaft 9 moves downwards, the groove 91 moves downwards at the same time, and then drives the bearing sleeve 671 to move downwards synchronously, and then drives the connecting plate 67 to move downwards, and then drives the detection head 65 to move downwards, so as to detect the liquid in the detection box, and transmit data to the computer terminal, when the detection head 65 moves downwards, the sliding disk 66 and the arc-shaped plate 661 can be driven to slide downwards synchronously, because the height of the cleaning mechanism 64 is limited by the static disk 63 and the limiting rod 631, the height is fixed, and simultaneously, the distance between the bottoms of the two arc-shaped plates 661 is smaller than the distance between the tops of the two arc-shaped plates 661, when the arc-shaped plates 661 move downwards gradually, the sliding head 643 slides from the bottom of the arc-shaped plate 661 to the top of the arc-shaped plate 645 gradually, and at this time, due to the elastic potential energy of the spring, the sliding head 643 can be pushed to move away from the detection head 65, and then the sliding rod drives the sliding rod 642 to move outwards, and further drives the two cleaning plates 644 to separate from the surface of the detection head 65 gradually, in a similar way, when detecting head 65 rebound, two cleaning board 644 can gradually with detect the laminating of head 65 outer wall this moment, will detect head 65 surface residual liquid stain and strike off then, prevent to influence down group's sample detection precision. The detection box after the detection is finished is then displaced to the position of the inclined plate 13, automatically falls down due to the action of gravity, and is convenient to collect in a centralized manner.
In summary, the invention provides a flow cytometer, which drives a reversing mechanism to rotate through the rotation of a motor, further drives a material changing disc to rotate, realizes a sequential feeding function, simultaneously drives an oscillation liquid injection mechanism to perform oscillation stirring on liquid to be detected through gear meshing transmission, prevents cell precipitation from influencing detection precision, simultaneously drives a feeding mechanism to perform automatic quantitative liquid injection on a detection box, simultaneously drives a detection mechanism to perform automatic detection on a sample, can automatically clean a detection head through a cleaning mechanism after detection is finished, improves detection precision, conveniently and intensively collects waste liquid after detection through an inclined plate, increases linkage between structures, and is more convenient to operate and has higher automation degree.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent changes made by using the contents of the present specification and the drawings, or applied directly or indirectly to the related technical fields, are included in the scope of the present invention.
Claims (7)
1. A flow cytometer, comprising: the automatic material changing device comprises a material changing disc and a reversing mechanism, wherein a plurality of notches are uniformly formed in the edge of the material changing disc along the circumferential direction; a mounting base is arranged between the material changing plate and the reversing mechanism;
the reversing mechanism comprises a rotary disc, a material shifting mechanism and a motor, the rotary disc is connected with the material changing disc through a rotating shaft, notches are uniformly formed in the edge of the rotary disc along the circumferential direction, and the number of the notches is the same as that of the notches;
the material shifting mechanism comprises an arc-shaped disc, a shifting rod and a roller, the bottom of the arc-shaped disc is connected with a motor, one end of the shifting rod is installed at the bottom of the arc-shaped disc, the other end of the shifting rod is connected with the roller, and the roller rotates the turntable through a notch in the rotating stroke of the shifting rod.
2. A flow cytometer as described in claim 1, wherein: the mounting base is fixedly provided with a storage cylinder, a detection box is arranged in the storage cylinder, and the detection box is cylindrical.
3. A flow cytometer according to claim 2 wherein: the ring channel has been seted up to installation base upper surface, the groove width size of ring channel with the external diameter of detection box is the same, the ring channel is provided with the baffle along the outer lane, the baffle is an unclosed ring, the unclosed department of baffle is provided with the swash plate that supplies detection box roll-off installation base.
4. A flow cytometer as described in claim 1, wherein: a first gear is mounted above the material changing plate through a rotating shaft, an oscillating liquid injection mechanism is fixedly mounted on the mounting base and comprises a first limiting plate, a first mounting plate, a steering cylinder, a convex block, a liquid storage tank, a second mounting plate and a feeding mechanism; the first limiting plate is arranged on the upper surface of the mounting base, a first mounting plate is arranged at one end, facing the annular groove, of the first limiting plate, a rotating rod is slidably mounted on the first mounting plate, a steering cylinder is arranged at the top of the rotating rod, and an inclined groove is formed in the outer wall of the steering cylinder; the convex block is fixedly arranged at one end of the first limiting plate, and a raised head of the convex block is arranged in the chute in a sliding manner; the bottom of the rotating rod is provided with a liquid storage tank, the bottom of the liquid storage tank is provided with a spline shaft, and the inside of the spline shaft is of a hollow structure and is communicated with the liquid storage tank; the upper end of the spline shaft is provided with a groove, a second gear meshed with the first gear is clamped on the outer wall of the spline shaft, a spline sleeve matched with the spline shaft is arranged in the middle of the second gear, a second mounting plate is arranged at the position, opposite to the spline sleeve, of one end of the first limiting plate, two groups of second mounting plates are arranged and are respectively positioned on the upper surface and the lower surface of the second gear, and the spline sleeve is movably connected with the second mounting plate; the integral key shaft lower extreme outer wall is provided with first piston plate, the integral key shaft bottom is provided with the second piston board, the liquid outlet has been seted up to the integral key shaft outer wall, the liquid outlet is located between first piston board and the second piston board, first piston board and second piston board stretch into in the feeding mechanism, feeding mechanism sets up the bottom at the second mounting panel.
5. A flow cytometer as described in claim 4 wherein: the feeding mechanism comprises a sealing barrel and a liquid guide pipe, the sealing barrel is arranged at the bottom of the second mounting plate, the first piston plate and the second piston plate extend into the sealing barrel, and the liquid guide pipe is arranged at the lower end of the sealing barrel.
6. A flow cytometer as described in claim 1, wherein: the mounting base is fixedly provided with an inspection mechanism, and the inspection mechanism comprises a second limiting plate, a third mounting plate, a static disc, a cleaning mechanism, a detection head, a sliding disc and a connecting plate; the second limiting plate is arranged on the upper surface of the mounting base, and a third mounting plate is arranged at one end, facing the annular groove, of the second limiting plate; a static disc is arranged at one end, away from the second limiting plate, of the third mounting plate, two limiting rods are symmetrically arranged at the bottom of the static disc, and the bottom of each limiting rod is connected with a cleaning mechanism; the detection head is electrically connected with the computer terminal, the outer wall of the detection head is fixedly connected with a sliding disc, the sliding disc is connected with the two limiting rods in a sliding mode, the bottom of the sliding disc is provided with two arc-shaped plates relative to the cleaning mechanism, and the distance between the bottoms of the two arc-shaped plates is smaller than that between the tops of the two arc-shaped plates; the top of the detection head is connected with one end of the connecting plate, the other end of the connecting plate is provided with a bearing sleeve matched with the groove, and the connecting plate is connected with the spline shaft and is mutually clamped with the groove through the bearing sleeve.
7. A flow cytometer as described in claim 6 wherein: the cleaning mechanism comprises a sliding sleeve, a sliding rod, a sliding head, a cleaning plate and a spring; the sliding sleeve is connected with the limiting rod, the sliding sleeve is slidably mounted on the sliding rod, a sliding head is arranged at one end of the sliding rod and is in contact with the arc-shaped plate, a spring is arranged between the sliding head and the sliding sleeve, and a cleaning plate is arranged at the other end of the sliding rod.
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| CN202110517278.6A CN113237819B (en) | 2021-05-12 | 2021-05-12 | Flow cytometry |
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| CN202110517278.6A CN113237819B (en) | 2021-05-12 | 2021-05-12 | Flow cytometry |
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