CN113234634A - Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene - Google Patents
Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene Download PDFInfo
- Publication number
- CN113234634A CN113234634A CN202110598572.4A CN202110598572A CN113234634A CN 113234634 A CN113234634 A CN 113234634A CN 202110598572 A CN202110598572 A CN 202110598572A CN 113234634 A CN113234634 A CN 113234634A
- Authority
- CN
- China
- Prior art keywords
- rhodococcus
- fluorene
- polycyclic aromatic
- phenanthrene
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- NIHNNTQXNPWCJQ-UHFFFAOYSA-N fluorene Chemical compound C1=CC=C2CC3=CC=CC=C3C2=C1 NIHNNTQXNPWCJQ-UHFFFAOYSA-N 0.000 title claims abstract description 42
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 title claims abstract description 36
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 title claims abstract description 28
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 241001425152 Rhodococcus aetherivorans Species 0.000 title claims abstract description 26
- 230000000593 degrading effect Effects 0.000 title description 4
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 claims abstract description 43
- 230000015556 catabolic process Effects 0.000 claims abstract description 28
- 238000006731 degradation reaction Methods 0.000 claims abstract description 28
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 238000009629 microbiological culture Methods 0.000 claims description 5
- 241000316848 Rhodococcus <scale insect> Species 0.000 abstract description 8
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052799 carbon Inorganic materials 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000003708 ampul Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000012880 LB liquid culture medium Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 150000001447 alkali salts Chemical class 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 206010043275 Teratogenicity Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 231100001239 persistent pollutant Toxicity 0.000 description 1
- 238000007539 photo-oxidation reaction Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 231100000211 teratogenicity Toxicity 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of microorganisms, in particular to rhodococcus aetherivorans and application thereof in degradation of four polycyclic aromatic hydrocarbons, wherein the used bacterial strain is rhodococcus aetherivorans (Rhodococcus aetherivorans), and the preservation number is CGMCC (No. 12425); the polycyclic aromatic hydrocarbon is selected from naphthalene, anthracene, phenanthrene and fluorene. The result shows that the rhodococcus aetheriformis can grow by using four polycyclic aromatic hydrocarbons as a unique carbon source under aerobic conditions, the rhodococcus aetheriformis has good degradation performance on phenanthrene and fluorene, and the degradation rate of the rhodococcus aetheriformis on fluorene is up to 74.22%. The strain screened by the invention can provide new germplasm resources for developing the research on degradation mechanisms of various polycyclic aromatic hydrocarbons.
Description
Technical Field
The invention belongs to the technical field of biological treatment of environmental organic pollutants, and particularly relates to rhodococcus aetherivorans and application thereof in degradation of naphthalene, anthracene, phenanthrene and fluorene.
Background
Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants with mutagenicity, carcinogenicity and teratogenicity, are not easily degraded under natural conditions, can be widely distributed in different environmental media, and pose great threat to ecological environment and human health. PAHs in the environment can be degraded through various ways such as volatilization, photooxidation, chemical oxidation, biodegradation and the like, wherein the microbial degradation can decompose or convert harmful PAHs into less harmful or nontoxic compounds, so that the PAHs have better safety and minimal interference to the environment. The research selects rhodococcus aetherivorans (Rhodococcus aetherivorans) separated from the polycyclic aromatic hydrocarbon polluted soil, and the rhodococcus aetherivorans is utilized to carry out degradation experiments on four polycyclic aromatic hydrocarbons, namely naphthalene, anthracene, phenanthrene and fluorene to determine the degradation capability of the rhodococcus aetherivorans so as to provide new germplasm resources for developing the degradation mechanism research of degradation of various polycyclic aromatic hydrocarbons.
Disclosure of Invention
The invention aims to overcome the problems of unclear variety and lack of degradation rate of the conventional polycyclic aromatic hydrocarbon degrading flora, a Rhodococcus aetherivorans (Rhodococcus aetherivorans) separated from polycyclic aromatic hydrocarbon polluted soil is purchased from the China general microbiological culture Collection center (http:// www.cgmcc.net /), the preservation number is CGMCC (1.12425), and the bacterial strain is used for degradation experiments to determine that the bacterial strain has higher degradation efficiency on four polycyclic aromatic hydrocarbons, namely naphthalene, anthracene, phenanthrene and fluorene, has good degradation effect and is environment-friendly.
In order to achieve the purpose, the technical scheme of the invention is as follows: the application of rhodococcus aetherivorans in degradation of polycyclic aromatic hydrocarbons is characterized in that the rhodococcus aetherivorans (Rhodococcus aetherivorans) is purchased from China general microbiological culture Collection center with the preservation number of CGMCC (1.12425).
In the application, the polycyclic aromatic hydrocarbon is one or more of naphthalene, anthracene, phenanthrene and fluorene.
The polycyclic aromatic hydrocarbon degrading bacteria provided by the invention are separated from polycyclic aromatic hydrocarbon polluted soil, are preserved in China general microbiological culture Collection center with the serial number of CGMCC (China general microbiological culture collection center) (1.12425), are Rhodococcus aetherivorans and are gram-positive.
Rhodococcus aetheriformis can grow and reproduce by using naphthalene, anthracene, phenanthrene and fluorene as unique carbon sources and energy sources, and the degradation rate of 50mg/L phenanthrene and fluorene in a Rhodococcus aetheriformis inorganic salt culture medium is over 50% under a pure culture condition.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the invention are not limited thereto.
EXAMPLE 1 growth Performance of Rhodococcus aetherivorans in polycyclic aromatic hydrocarbons System
Sterilizing the outer surface of an ampoule by using 75% alcohol absorbent cotton, heating the top end of the ampoule by using flame, dropping a small amount of sterile water to the top end of the heated ampoule to break the ampoule (the influence of excessive water vapor on strain recovery is avoided), knocking down the top end of the broken ampoule by using tweezers, sucking about 0.3mL of corresponding LB liquid culture medium (table 1, no agar is added) by using a sterile straw, adding the LB liquid culture medium into the ampoule, and slightly shaking to dissolve freeze-dried bacteria to be in a suspension state. And (3) sucking all the bacterial suspension, transplanting the bacterial suspension into an activation medium (table 1) test tube, and placing the test tube at the constant temperature of 30 ℃ for culturing until bacterial colonies grow out, which indicates that the bacterial strains are successfully activated. The successfully activated strains are subcultured.
TABLE 1 strains and activation media used in the experiments
Secondly, inoculating the successfully activated strains into an LB enrichment medium, wrapping a bottle mouth with a biological sealing film to isolate sundry bacteria, and culturing in a constant-temperature oscillation incubator at 30 ℃ and 150r/min for 16-20h to logarithmic phase.
Thirdly, accurately weighing 50mg of PAHs standard substances (naphthalene, anthracene, phenanthrene and fluorene) by using an analytical balance, putting the PAHs standard substances into a clean beaker, dissolving the PAHs standard substances by using chromatographic grade methanol, transferring the solution into a volumetric flask with the specification of 100mL, fixing the volume to a scale mark by using the methanol, and shaking up to obtain 500mg/L mother liquor of the PAHs (naphthalene, anthracene, phenanthrene and fluorene).
And fourthly, determining the absorption wavelengths of the naphthalene, the anthracene, the phenanthrene and the fluorene used in the experiment by using an ultraviolet spectrophotometer. Then, an ultraviolet spectrophotometer is used for respectively measuring the absorbance values of four PAHs with the concentrations of 2, 4, 6, 8 and 10mg/L, a standard curve is obtained by taking the concentration value of a PAHs standard solution as an X axis and the corresponding ultraviolet absorbance value as a Y axis, and a regression equation and R of the standard curves of naphthalene, anthracene, phenanthrene and fluorene2Respectively as follows:
y＝0.0111*x-0.0102，R2becoming 0.9971 (naphthalene)
y＝0.0076*x+0.0896，R2Becoming 0.9952 (anthracene)
y 0.0102 x +0.0028, R2 0.9985 (phenanthrene)
y 0.0099 x-0.0056, R2 0.9923 (fluorene)
Of the formulae R2All are more than 0.99, which shows that the linear fitting degree is good and the accurate quantitative analysis can be realized.
Fifthly, filtering and sterilizing the four mother solutions by using a 0.22 mu m organic filter membrane, placing the four mother solutions in a fume hood for blowing until the solvent is completely volatilized, and then adding a liquid basic salt culture medium according to the concentration proportion, wherein the final concentration of the four PAHs in the culture system is 50 mg/L. Then diluting the strain cultured to logarithmic phase (16-20h) in the step II with an inorganic salt culture medium on a sterile workbench, and measuring the initial OD600And respectively inoculating the mixture into a culture system, and placing the culture system into a constant-temperature shaking table at 30 ℃ for shake culture at 150rpm for 7 days. Meanwhile, the degradation condition is observed, if PAHs adhered to the wall of the triangular flask are found, the triangular flask is shaken to dissolve the PAHs on the wall of the flask in a degradation system, a contrast test is carried out on a bacteria-containing basic salt culture medium without adding polycyclic aromatic hydrocarbon mother liquor, and three groups of experiments are arranged in parallel.
Sixthly, after culturing for 7 days, sampling one by one to a quartz cuvette, and measuring OD by using a spectrophotometer600By passingAnd (5) primarily judging the degradation condition by the growth amount. The growth amounts of Rhodococcus aetherivorans in mineral salt media containing four PAHs, respectively, are shown in Table 2.
TABLE 2 growth of Rhodococcus aetherivorans in mineral salts medium containing four PAHs
Since inorganic salts belong to the sole carbon source in the medium, PAHs belong to the sole nutrient. Therefore, the growth nutrient source of Rhodococcus aetherivorans is polycyclic aromatic hydrocarbons contained therein, and as can be seen from Table 2, the OD of Rhodococcus aetherivorans in all polycyclic aromatic hydrocarbon systems during the culture period600All rise.
This example illustrates that Rhodococcus aetherivorans can grow using four polycyclic aromatic hydrocarbons as the sole carbon source.
Example 2 degradation of polycyclic aromatic hydrocarbons in a System by Rhodococcus aetherivorans
Extracting the culture solution cultured for 7 days in example 1 by using ethyl acetate, centrifuging for 10min at 4000rpm/min and 4 ℃, taking the supernatant, filtering the supernatant by using anhydrous sodium sulfate, diluting the supernatant by a certain multiple, determining the absorbance under the corresponding wavelength, taking the average value of the absorbance of three groups of samples, respectively substituting the average value into a regression equation of a standard curve, and calculating to obtain the residual concentrations and degradation rates of the four degraded PAHs, wherein the results are shown in Table 3. As can be seen from Table 3, the non-biodegradation effect of the four polycyclic aromatic hydrocarbons is very small, the degradation rate of phenanthrene and fluorene is over 50%, and the degradation rate of rhodococcus aetheriformis on fluorene is as high as 62.08%.
TABLE 3 concentration of PAHs remaining in the degradation system and degradation rate thereof
This example shows that Rhodococcus aetheriformis has a degradation capability on four kinds of polycyclic aromatic hydrocarbons, phenanthrene and fluorene can be degraded at a high level, and the degradation rate of Rhodococcus aetheriformis on fluorene is up to 62.08%.
Claims (2)
1. The application of rhodococcus aetherivorans in degradation of polycyclic aromatic hydrocarbons is characterized in that the rhodococcus aetherivorans (Rhodococcus aetherivorans) is purchased from China general microbiological culture Collection center with the preservation number of CGMCC (1.12425).
2. The use according to claim 1, wherein the polycyclic aromatic hydrocarbon is one or more of naphthalene, anthracene, phenanthrene, and fluorene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110598572.4A CN113234634A (en) | 2021-05-31 | 2021-05-31 | Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110598572.4A CN113234634A (en) | 2021-05-31 | 2021-05-31 | Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113234634A true CN113234634A (en) | 2021-08-10 |
Family
ID=77135884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110598572.4A Pending CN113234634A (en) | 2021-05-31 | 2021-05-31 | Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113234634A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196589A (en) * | 2021-12-23 | 2022-03-18 | 浙江工业大学 | Rhodococcus aetherivorans ZHC and application thereof in degradation of methyl acrylate |
| CN116179402A (en) * | 2022-09-06 | 2023-05-30 | 南京工业大学 | Carotenoid synthetic strain and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120178146A1 (en) * | 2009-04-03 | 2012-07-12 | IFP Energies Nouvelles | Bacteria capable of degrading multiple petroleum compounds in solution in aqueous effluents and process for treating said effluents |
| CN109517780A (en) * | 2018-11-12 | 2019-03-26 | 沈阳药科大学 | The method for improving the Rhod quasi-microorganism speed of growth and preventing spawn degeneration |
| CN115537446A (en) * | 2022-09-06 | 2022-12-30 | 天津中医药大学 | Application of rhodococcus aetherivorans in production of 4-androstenedione by converting cholesterol |
-
2021
- 2021-05-31 CN CN202110598572.4A patent/CN113234634A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120178146A1 (en) * | 2009-04-03 | 2012-07-12 | IFP Energies Nouvelles | Bacteria capable of degrading multiple petroleum compounds in solution in aqueous effluents and process for treating said effluents |
| CN109517780A (en) * | 2018-11-12 | 2019-03-26 | 沈阳药科大学 | The method for improving the Rhod quasi-microorganism speed of growth and preventing spawn degeneration |
| CN115537446A (en) * | 2022-09-06 | 2022-12-30 | 天津中医药大学 | Application of rhodococcus aetherivorans in production of 4-androstenedione by converting cholesterol |
Non-Patent Citations (9)
| Title |
|---|
| CGMCC中国普通微生物菌种保藏中心: "CGMCC 1.12425", pages 1, Retrieved from the Internet <URL:https://cgmcc.net/directory/detail?cgmccid=1.12425&number=1.12425&genus=&speci> * |
| JIE QU ET AL.: "Complete Genome Sequence of Rhodococcus sp. Strain IcdP1 Shows Diverse Catabolic Potential", 《GENOME ANNOUNC》, pages 00711 - 15 * |
| LI-LI MIAO ET AL.: "Hydroxylation at Multiple Positions Initiated the Biodegradation of Indeno[1,2,3-cd]Pyrene in Rhodococcus aetherivorans IcdP1", 《FRONT. MICROBIOL.》, vol. 11, pages 3 * |
| MARC AUFFRET ET AL.: "Degradation of a mixture of hydrocarbons, gasoline, and diesel oil additives by Rhodococcus aetherivorans and Rhodococcus wratislaviensis", 《APPL ENVIRON MICROBIOL》, pages 7774 - 7782 * |
| PENG WU ET AL.: "Fluorene degradation by Rhodococcus sp. A2-3 isolated from hydrocarbon contaminated sediment of the Pearl River estuary, China", 《ECOTOXICOLOGY》, pages 929 - 935 * |
| 卢晓霞 等: "焦化厂污染土壤中多环芳烃降解菌的分离及降解特性", 《应用与环境生物学报》, pages 722 - 726 * |
| 夏瑛铭 等: "外加碳源对红球菌IcdP1降解荧蒽的特性研究", 《环境科学与技术》, pages 14 - 19 * |
| 环境保护部自然生态保护司编: "《土壤污染与人体健康》", 中国环境科学出版社, pages: 302 * |
| 秦富军: "红球菌P14中参与多环芳烃降解的新型双加氧酶功能分析", 《万方数据知识服务平台》, pages 1 - 74 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196589A (en) * | 2021-12-23 | 2022-03-18 | 浙江工业大学 | Rhodococcus aetherivorans ZHC and application thereof in degradation of methyl acrylate |
| CN114196589B (en) * | 2021-12-23 | 2023-05-23 | 浙江工业大学 | Rhodococcus etherae ZHC and application thereof in degradation of methyl acrylate |
| CN116179402A (en) * | 2022-09-06 | 2023-05-30 | 南京工业大学 | Carotenoid synthetic strain and application thereof |
| CN116179402B (en) * | 2022-09-06 | 2024-01-30 | 南京工业大学 | Carotenoid synthetic strain and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Przystaś et al. | Biological removal of azo and triphenylmethane dyes and toxicity of process by-products | |
| Liu et al. | Effect of plant density on phytoremediation of polycyclic aromatic hydrocarbons contaminated sediments with Vallisneria spiralis | |
| CN113234634A (en) | Rhodococcus aetherivorans and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene | |
| Sarim et al. | Biosorption of direct textile dye Congo red by Bacillus subtilis HAU-KK01 | |
| Chaieb et al. | Biodegradation and decolorization of azo dyes by adherent Staphylococcus lentus strain | |
| CN114540226B (en) | Polycyclic aromatic hydrocarbon degrading strain LJB-25 in petroleum polluted soil, and microbial inoculum and application thereof | |
| CN1793311A (en) | Facultative anaerobe of degrading polychlorinated diphenyl and obtainding process | |
| CN114107092B (en) | Endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN103013885A (en) | Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof | |
| CN112940972B (en) | Endophytic bacteria PX1 with polycyclic aromatic hydrocarbon degradation function and application thereof | |
| Aruna et al. | Decolorization of Acid Blue 25 dye by individual and mixed bacterial consortium isolated from textile effluents | |
| CN110317745B (en) | Ralstonia pickettii M1 strain and application thereof in degrading phenanthrene and biphenyl | |
| CN104805018B (en) | Earth mould (Agromyces sp.) MT E of one plant of a variety of phthalic acid ester that can degrade simultaneously | |
| CN104845890B (en) | Applications of earth mould (Agromyces sp.) the MT E in a variety of phthalic acid esters of degrading | |
| CN101880642B (en) | Ralstoniapickettii H2 with chlorobenzene degrability and applications thereof | |
| CN116656535A (en) | Bacterial strain with chlorohydrocarbon degradation capability and application thereof | |
| CN113336334A (en) | Bacillus firmus and application thereof in degrading naphthalene, anthracene, phenanthrene and fluorene | |
| Nahar et al. | Toxic effects of toluene on the growth of activated sludge bacteria | |
| Khazaal et al. | Bioremediation and detoxification of real refinery oily sludge using mixed bacterial cells | |
| Mahmoodsaleh et al. | Methyl tertiary butyl ether biodegradation by the bacterial consortium isolated from petrochemical wastewater and contaminated soils of Imam Khomeini Port Petrochemical Company (Iran) | |
| CN115786179A (en) | Bacterial strain for degrading o-dichlorobenzene and application thereof | |
| TWI589694B (en) | Acinetobacter junii ds44 isolate having the benzene and/or naphthalene degrading ability and uses of the same | |
| CN110433619B (en) | Application of Aeromonas salmonicida subspecies in degradation of volatile organic pollutants | |
| CN104845891B (en) | A kind of earth mould bacteria suspension of a variety of phthalic acid esters of degrading and its application | |
| CN113755371B (en) | Strain A.seifertigii P52-1 and application thereof in degradation of polychlorinated biphenyl |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |