CN113231112A - Microfluidic chip reagent storage device and application method thereof - Google Patents
Microfluidic chip reagent storage device and application method thereof Download PDFInfo
- Publication number
- CN113231112A CN113231112A CN202110450479.9A CN202110450479A CN113231112A CN 113231112 A CN113231112 A CN 113231112A CN 202110450479 A CN202110450479 A CN 202110450479A CN 113231112 A CN113231112 A CN 113231112A
- Authority
- CN
- China
- Prior art keywords
- reagent
- interface
- microfluidic chip
- storage device
- interface structure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a microfluidic chip reagent storage device and a using method thereof, wherein the microfluidic chip reagent storage device comprises: reagent storage bag container, reagent end cap card strip and install the reagent interface connection card strip at the equipment end, at least a part of reagent storage bag container is flexible structure, reagent storage bag container includes at least one independent reagent and bears the weight of the chamber, and set up the interface structure in the entrance that the chamber was born to the reagent, reagent end cap card strip and interface structure detachably sealing connection, the inside of reagent storage bag container is the reagent circulation passageway that the bottom in every reagent bore the weight of the chamber communicates, the upper end that the passageway was circulated to the reagent communicates with the entry in reagent bore the weight of the chamber intercommunication, interface structure can dismantle and be connected with reagent interface connection card strip and sealedly after dismantling reagent end cap card strip. The invention can meet different application scenes of disposable use, repeated use or repeated use, has the characteristics of reversible disassembly and plug-and-play, and can avoid the problems of cross contamination, loss and the like of the reagent due to the sealed storage of the reagent.
Description
Technical Field
The invention belongs to the field of microfluidic chip reagent storage devices, and particularly relates to a microfluidic chip reagent storage device and a using method thereof.
Background
The micro-fluidic chip is a rapid analysis platform which can integrate basic operations such as sample preparation, reaction, separation, detection and the like involved in the biological, chemical and medical analysis processes. Based on the characteristics of small volume, low reagent consumption, high integration and the like, the micro-fluidic chip has great application potential in the fields of biology, chemistry, medical treatment and the like, and has gradually developed into a research field of high cross fusion of biochemistry, hydrodynamics, microelectronics, materials, machinery and the like.
Since the inside of the microfluidic chip relates to various biochemical reactions, the introduction of the fluid reagent from the outside is an important link for realizing the functions of the microfluidic chip. Currently, most of the external reagents of microfluidic chips are still manually filled, injected, or implemented by using a syringe/pump for carrying the reagents. However, such methods can greatly weaken the inherent advantages of automation, miniaturization and portability of the microfluidic chip. Part of the microfluidic chip adopts the integrated design of the reagent and the chip, and the method can greatly simplify peripheral liquid supply equipment of the microfluidic chip, but has the following three problems:
(1) micro valves are required to be arranged in the chip to realize time sequence supply of different reagents, the complexity of the chip structure is improved, and the process cost is improved, and the reliability and the yield are reduced; (2) the size of the chip is often incompatible with the volume of the reagent and cannot bear the required dosage of the reagent; (3) the integrated packaging requirements of the liquid reagent and the microfluidic chip are high, pressure loss and liquid leakage are easily caused, and the manufacturing cost of the chip is also increased.
Disclosure of Invention
The invention aims to provide a microfluidic chip reagent storage device and a using method thereof, which can meet different application scenes of disposable use, repeated use or repeated use and have the characteristics of reversible disassembly and plug-and-play.
In order to achieve the above object, according to an aspect of the present invention, there is provided a microfluidic chip reagent storage device including: reagent storage bag container, reagent end cap card strip and install the reagent interface connection card strip at the equipment end, at least a part of reagent storage bag container is flexible structure, reagent storage bag container includes that at least one independent reagent bears the weight of the chamber and sets up the interface structure of the entrance in reagent bearing chamber, reagent end cap card strip with interface structure detachably sealing connection, the inside of reagent storage bag container is every the reagent circulation passageway of the bottom intercommunication in reagent bearing chamber, the upper end of reagent circulation passageway with the entry intercommunication in reagent bearing chamber, interface structure is demolising after the reagent end cap card strip can with reagent interface connection card strip can dismantle and connect sealedly.
According to the invention, the reagent storage capsule container comprises two layers of film which are bonded in the circumferential direction of the reagent carrying chamber and are separated from each other in the region of the reagent carrying chamber.
According to the invention, the microfluidic chip reagent storage device further comprises a reagent interface fixing clamping strip, the reagent interface fixing clamping strip is fixedly connected with the reagent storage bag container, and the interface structure is formed on the reagent interface fixing clamping strip.
According to the invention, a reagent tube used as the reagent flow channel is arranged in the reagent storage capsule container, the reagent tube is arranged in the reagent bearing cavity, one end of the reagent tube is hermetically connected with the interface structure, and the other end of the reagent tube extends to the bottom of the reagent bearing cavity.
According to the invention, the reagent storage capsule container comprises a reagent bag back plate and reagent cavity membranes, wherein the reagent cavity membranes are hermetically connected in the circumferential direction of each reagent bearing cavity and are separated from each other in the area where the reagent bearing cavity is located.
According to the invention, a pore canal used as the reagent flowing channel is formed inside the reagent bag back plate, one end of the pore canal is hermetically connected with the interface structure, and the other end of the pore canal extends to the bottom of the reagent bearing cavity.
Preferably, the interface structure is integrally formed with the reagent bag backing plate.
According to the invention, the interface structure adopts a standard luer male interface, the reagent plug clamping strip comprises a plug structure, and the plug structure adopts a standard luer plug which can be screwed with the interface structure, or adopts a conical plug, or adopts a structure which can be in direct-insertion sealing connection with the interface structure.
According to the invention, the reagent interface connecting strip is provided with a reagent connecting port which can be hermetically connected with the interface structure in a direct insertion mode, the rear end of the reagent interface connecting strip is provided with a liquid connecting structure, and preferably, the liquid connecting structure adopts a threaded interface or a luer connector.
According to another aspect of the present invention, there is also provided a method for using any one of the microfluidic chip reagent storage devices described above, including the steps of:
step 2, removing the reagent plug clamping strip from the interface structure, directly connecting the interface structure with the reagent loading module, separating the interface structure from the reagent loading module after filling a preset reagent volume, and completely sealing the interface structure by using the reagent plug clamping strip again;
step 3, when the reagent is to be used, removing the reagent plug clamping strip, connecting the reagent interface connecting clamping strip to the interface structure, wherein the liquid connecting structure of the reagent interface connecting clamping strip is used for being connected with the pipeline, and if the pipeline has an active driving source, the driving source is used as power to realize the supply of the reagent in the microfluidic chip reagent storage device; if the pipeline interface has no power source, the reagent in the microfluidic chip reagent storage device is supplied by extruding the reagent storage capsule container.
The invention has the beneficial effects that: due to the adoption of the technical scheme, the invention can meet different application scenes of disposable use, repeated use or repeated use, has the characteristics of reversible disassembly and plug-and-play, can realize the sealed storage of the reagent, can avoid the problems of cross contamination, loss and the like of the reagent, and can realize the storage and supply of the liquid of various chips by matching with a subsequent liquid supply module.
Drawings
FIG. 1 shows a schematic structural diagram of a first embodiment of the present invention;
FIG. 2 shows a cross-sectional view A-A and B-B of FIG. 1;
FIG. 3 shows a schematic structural view of a reagent plug strip;
FIG. 4 shows a schematic structural diagram of a reagent interface connection card strip;
FIG. 5 shows a schematic structural diagram of a second embodiment of the present invention;
fig. 6 shows a cross-sectional view a-a and B-B in fig. 5.
Reference numbers in the figures: 1. a reagent storage capsule container; 2. a reagent plug clamping strip; 3. the reagent interface is connected with the clamping strip; 4. a reagent carrying chamber; 5. an inlet; 6. an interface structure; 7. a film; 8. fixing a clamping strip on the reagent interface; 9. a reagent tube; 10. a reagent bag backing plate; 11. a duct; 12. a plug structure; 13. a reagent connection port; 14. a liquid connection structure; 15. a reagent chamber membrane.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and embodiments. It should be emphasized that the specific embodiments described herein are merely illustrative of the invention, are some, not all, and therefore do not limit the invention. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
According to one aspect of the present invention, a microfluidic chip reagent storage device is provided, which can extract or load a reagent in a reagent storage structure through direct pressure deformation or indirect air pressure driving, thereby realizing stable reagent supply.
Referring to fig. 1 to 6, the microfluidic chip reagent storage device according to the present invention includes: the reagent storage bag comprises a reagent storage bag container 1, a reagent plug clamping strip 2 and a reagent interface connecting clamping strip 3 arranged at the equipment end, wherein at least one part of the reagent storage bag container 1 is of a flexible structure. The reagent storage bag container 1 comprises at least one independent reagent bearing cavity 4 and an interface structure 6 arranged at an inlet 5 of the reagent bearing cavity 4, a reagent plug clamping strip 2 is detachably and hermetically connected with the interface structure 6, a reagent circulation channel communicated with the bottom of each reagent bearing cavity 4 is arranged in the reagent storage bag container 1, the upper end of the reagent circulation channel is communicated with the inlet of the reagent bearing cavity 4, and the interface structure 6 can be detachably and hermetically connected with a reagent interface connection clamping strip 3 after the reagent plug clamping strip 2 is detached.
According to the present invention, the reagent holding chambers 4 hold reagents, and the reagent storage pouch container 1 may have a multi-row structure of a plurality of reagent holding chambers 4 formed thereon, so that there are a plurality of reagent holding chambers 4, and the inlet 5 corresponds to the position of the interface structure 6. When the reagent plug clamping strip is delivered from a factory, the reagent plug clamping strip 2 is installed at the interface structure 6 and is detachably connected with the interface structure 6 in a sealing mode, and therefore reagents are sealed in the respective reagent bearing cavities 4. The reagent interface connection clamping strip 3 is arranged at the equipment end, when in use, the reagent plug clamping strip 2 is taken down from the reagent storage bag container 1, so that the inlet 5 of the reagent bearing cavity 4 is in an open state, and then the reagent storage bag container 1 is arranged on the reagent interface connection clamping strip 3 through the interface structure 6 on the reagent storage bag container, so that the equipment end can draw out the reagent through the inlet 5 and the reagent circulation channel for use.
Due to the adoption of the technical scheme, the invention can meet different application scenes of disposable use, repeated use or repeated use, has the characteristics of reversible disassembly and plug-and-play, can realize the sealed storage of the reagent, can avoid the problems of cross contamination, loss and the like of the reagent, and can realize the storage and supply of the liquid of various chips by matching with a subsequent liquid supply module.
Referring to fig. 1-4, a 5-gang configuration is shown in a first embodiment. The reagent storage capsule container 1 comprises two films 7, the two films 7 being bonded in the circumferential direction of the reagent holding chamber 4 and separated from each other in the region of the reagent holding chamber 4. Wherein, the membrane 7 can be made of PE, PP, PETC and other flexible structures. The area separated by the two films 7 is a reagent bearing cavity 4, and the function of bearing the reagent can be realized; the area where the two films 7 are bonded is a reagent cavity fixing structure and provides fixing and supporting capacity for the reagent bag.
Preferably, the microfluidic chip reagent storage device further comprises a reagent interface fixing clamping strip 8, the reagent interface fixing clamping strip 8 is fixedly connected with the reagent storage capsule container 1, and the interface structure 6 is formed on the reagent interface fixing clamping strip 8.
A reagent tube 9 serving as a reagent flow channel is arranged in the reagent storage capsule container 1, the reagent tube 9 is arranged in the reagent bearing cavity 4, wherein one end of the reagent tube 9 is hermetically connected with the interface structure 6, and the other end extends to the bottom of the reagent bearing cavity 4. Preferably, the reagent vessel 9 has an outer diameter in the range of 1.6mm to 2mm and an inner diameter in the range of 0.25mm to 1 mm. In order to ensure the sealing performance, irreversible encapsulation can be realized by adopting modes such as bonding, welding or integrated injection molding. The material of the reagent tube 9 can be selected from PTFE, ETFE, PFA, FEP, silica gel and other hard or flexible polymer materials. The membrane 7 in this embodiment is made of a flexible material, occupies a small space, and is suitable for storing expensive reagents with a small reagent consumption.
In order to reduce the processing difficulty, the present invention also provides a second embodiment, in which a case of a 5-gang structure is shown. Referring specifically to fig. 5-6, the reagent storage capsule container 1 comprises a reagent bag backing plate 10 and reagent chamber membranes 15, the reagent chamber membranes 15 being sealingly connected in the circumferential direction of each reagent carrying chamber 4 and separated from each other in the region where the reagent carrying chamber 4 is located. The reagent bag back plate 10 is made of hard material, and can be made of PP, PE, Teflon, polytetrafluoroethylene and the like. The reagent cavity membrane 15 and the reagent bag back plate 10 form a reagent bearing cavity 4, the material of the reagent cavity membrane 15 preferably has flexible materials such as PE, PP, PETC and the like, and structural integration is carried out by adopting modes such as laser welding, hot-press bonding and the like. The upper part of the reagent bag back plate 10 is provided with an interface structure 6, and the interface structure 6 and the reagent bag back plate 10 can be integrally formed.
A pore canal 11 used as a reagent flowing channel is formed inside the reagent bag back plate 10, one end of the pore canal 11 is hermetically connected with the interface structure 6, and the other end extends to the bottom of the reagent bearing cavity 4. The interface structure 6 is communicated with the physical space of the pore canal 11, and the lower end opening of the pore canal 11 is formed at the bottom of the reagent bag back plate 10.
The structure of the interface structure 6 and the reagent interface connection strip 3 of the present invention will be described in detail below.
The interface structure 6 adopts a standard luer male interface and can be matched with a standard syringe interface and a luer female interface. The reagent plug clamping strip 2 comprises a plug structure 12, wherein the plug structure 12 adopts a standard luer plug which can be screwed with the interface structure 6, or adopts a conical plug, or adopts a structure which can realize sealing connection with the interface structure 6 in a direct-insertion mode.
Preferably, a reagent connecting port 13 capable of being in sealing connection with the interface structure 6 in a straight-insertion manner is arranged on the reagent interface connecting clamping strip 3, a liquid connecting structure 14 is arranged at the rear end of the reagent interface connecting clamping strip 3, preferably, the liquid connecting structure 14 adopts a threaded interface or a luer connector, the threaded interface can be used for being matched with an inverted cone connector to connect a hard liquid conduit, and the luer connector can be used for being matched with a soft conduit.
By adopting the technical scheme, the invention realizes the airtight storage of the reagent and avoids the problems of cross contamination, loss and the like of the reagent; the liquid storage and supply of various chips can be realized by matching with a subsequent liquid supply module; the liquid storage and supply device can meet different application scenes of disposable use, repeated use or permanent use, has the characteristics of reversible disassembly and plug and play, and can realize the liquid storage and supply of various chips by matching with a subsequent liquid supply module.
The invention also provides a using method of the microfluidic chip reagent storage device, which comprises the following steps:
And 2, removing the reagent plug clamping strip 2 from the interface structure 6, directly connecting the interface structure 6 with the reagent loading module, separating the interface structure 6 from the reagent loading module after the preset reagent volume is filled, and completely sealing the interface structure 6 by using the reagent plug clamping strip 2 again.
Step 3, when the reagent is to be used, removing the reagent plug clamping strip 2, connecting the reagent interface connecting clamping strip 3 to the interface structure 6, connecting the liquid connecting structure 14 of the reagent interface connecting clamping strip 3 with a pipeline, and if the pipeline has an active driving source, using the driving source as power to realize the supply of the reagent in the microfluidic chip reagent storage device; if the pipeline interface has no power source, the reagent in the microfluidic chip reagent storage device is supplied by extruding the reagent storage capsule container 1.
All the preparation processes of all the parts and the parts related to the invention are general processes, such as injection molding, blow molding, machining, 3D printing, hot pressing, laser bonding and the like, and different processing process combinations can be selected according to different application occasions, structure precision and material requirements. In order to ensure that the interior of the reagent storage cavity is clean, the gas in the interior of the reagent bearing cavity can be completely discharged in a physical extrusion mode, namely the volume of the reagent bearing cavity is compressed to the minimum, and the interface structure 6 is completely sealed by using the reagent plug clamping strip 2.
Before the microfluidic chip reagent storage device is used, reagent loading is required, and the loading method can be realized by, for example, removing the reagent plug clamping strip 2 connected with the interface structure 6, directly connecting the interface structure 6 with a reagent loading module (the reagent loading module can be a universal injector or a special industrial-grade reagent loading device, and the like), and separating the interface structure 6 from the reagent loading module after a preset reagent volume is loaded. Then, the interface structure 6 is completely sealed by using the reagent plug clamping strip 2, and the reagent plug clamping strip is placed in an environment meeting the storage requirement of the reagent for storage and transportation.
When the reagent is to be used, the microfluidic chip reagent storage device is taken out, the reagent plug clamping strip 2 is removed, and the reagent interface connecting clamping strip 3 is connected to the interface structure 6. The liquid connecting structure 14 of the reagent interface connecting clamping strip 3 can be connected with a hard or soft pipeline, if an active driving source exists in the pipeline, the driving source can be used as power to realize the supply of the reagent in the reagent storage structure; if the pipeline interface has no power source, the reagent in the reagent storage structure can be supplied by extruding the bag.
The foregoing is only a preferred application of the present invention, and it should be noted that, for those skilled in the art, various modifications and improvements can be made without departing from the technical principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.
Claims (10)
1. A microfluidic chip reagent storage device, comprising: a reagent storage bag container (1), a reagent plug clamping strip (2) and a reagent interface connecting clamping strip (3) arranged at the equipment end, at least a part of the reagent storage capsule container (1) is of a flexible construction, the reagent storage capsule container (1) comprising at least one separate reagent carrying chamber (4) and an interface construction (6) arranged at an inlet (5) of the reagent carrying chamber (4), the reagent plug clamping strip (2) is detachably connected with the interface structure (6) in a sealing way, the interior of the reagent storage capsule container (1) is a reagent flow channel communicated with the bottom of each reagent bearing cavity (4), the upper end of the reagent flow channel is communicated with the inlet of the reagent bearing cavity (4), the interface structure (6) can be detachably and hermetically connected with the reagent interface connection clamping strip (3) after the reagent plug clamping strip (2) is removed.
2. Microfluidic chip reagent storage device according to claim 1, wherein the reagent storage capsule container (1) comprises two membranes (7), the two membranes (7) being bonded in the circumferential direction of the reagent carrying chamber (4) and separated from each other in the area where the reagent carrying chamber (4) is located.
3. The microfluidic chip reagent storage device according to claim 2, further comprising a reagent interface fixing clip strip (8), wherein the reagent interface fixing clip strip (8) is fixedly connected with the reagent storage capsule container (1), and the interface structure (6) is formed on the reagent interface fixing clip strip (8).
4. The microfluidic chip reagent storage device according to claim 2, wherein a reagent tube (9) serving as the reagent flow channel is arranged in the reagent storage capsule container (1), the reagent tube (9) is arranged in the reagent carrying cavity (4), one end of the reagent tube (9) is connected with the interface structure (6) in a sealing manner, and the other end of the reagent tube extends to the bottom of the reagent carrying cavity (4).
5. Microfluidic chip reagent storage device according to claim 1, wherein the reagent storage capsule container (1) comprises a reagent bag back plate (10) and a reagent cavity membrane (15), the reagent cavity membrane (15) being sealingly connected in the circumferential direction of each reagent carrying cavity (4) and separated from each other in the area where the reagent carrying cavity (4) is located.
6. The microfluidic chip reagent storage device according to claim 5, wherein the reagent bag back plate (10) is internally formed with a hole channel (11) serving as the reagent flow channel, one end of the hole channel (11) is connected with the interface structure (6) in a sealing manner, and the other end extends to the bottom of the reagent bearing cavity (4).
7. Microfluidic chip reagent storage device according to claim 6, wherein the interface structure (6) is integrally formed with the reagent bag backing plate (10).
8. The microfluidic chip reagent storage device according to any one of claims 1 to 7, wherein the interface structure (6) is a standard luer male interface, the reagent stopper strip (2) comprises a stopper structure (12), and the stopper structure (12) is a standard luer stopper which can be screwed with the interface structure (6), or a tapered stopper, or a structure which can be inserted in a straight manner with the interface structure (6) to realize a sealing connection.
9. The microfluidic chip reagent storage device according to any one of claims 1 to 7, wherein the reagent interface connection card strip (3) is provided with a reagent connection port (13) capable of being hermetically connected with the interface structure (6) in an in-line manner, and the rear end of the reagent interface connection card strip (3) is provided with a liquid connection structure (14), preferably the liquid connection structure (14) is a screw interface or a luer connector.
10. A method of using the microfluidic chip reagent storage device of any one of claims 1-9, comprising the steps of:
step 1, completely exhausting gas in a reagent bearing cavity (4) in a physical extrusion mode, so that the volume of the reagent bearing cavity (4) is compressed to the minimum, and a reagent plug clamping strip (2) is used for completely sealing an interface structure (6);
step 2, removing the reagent plug clamping strip (2) from the interface structure (6), directly connecting the interface structure (6) with the reagent loading module, separating the interface structure (6) from the reagent loading module after filling a preset reagent volume, and completely sealing the interface structure (6) by using the reagent plug clamping strip (2) again;
step 3, when the reagent is to be used, removing the reagent plug clamping strip (2), connecting the reagent interface connecting clamping strip (3) to the interface structure (6), connecting a liquid connecting structure (14) of the reagent interface connecting clamping strip (3) with a pipeline, and if an active driving source exists in the pipeline, supplying the reagent in the microfluidic chip reagent storage device by taking the driving source as power; if the pipeline interface has no power source, the reagent in the microfluidic chip reagent storage device is supplied by extruding the reagent storage capsule container (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110450479.9A CN113231112A (en) | 2021-04-25 | 2021-04-25 | Microfluidic chip reagent storage device and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110450479.9A CN113231112A (en) | 2021-04-25 | 2021-04-25 | Microfluidic chip reagent storage device and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113231112A true CN113231112A (en) | 2021-08-10 |
Family
ID=77129181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110450479.9A Pending CN113231112A (en) | 2021-04-25 | 2021-04-25 | Microfluidic chip reagent storage device and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113231112A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114405449A (en) * | 2022-02-19 | 2022-04-29 | 鲍熙来 | Chemical experiment device based on micro-fluidic |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207786624U (en) * | 2017-11-27 | 2018-08-31 | 深圳华炎微测医疗科技有限公司 | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system |
| CN210146038U (en) * | 2019-04-24 | 2020-03-17 | 浙江警察学院 | Modular sample single-channel detection chip assembly of handheld SPR detector |
-
2021
- 2021-04-25 CN CN202110450479.9A patent/CN113231112A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207786624U (en) * | 2017-11-27 | 2018-08-31 | 深圳华炎微测医疗科技有限公司 | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system |
| CN210146038U (en) * | 2019-04-24 | 2020-03-17 | 浙江警察学院 | Modular sample single-channel detection chip assembly of handheld SPR detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114405449A (en) * | 2022-02-19 | 2022-04-29 | 鲍熙来 | Chemical experiment device based on micro-fluidic |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102047124B (en) | Fluid processing and transfer using inter-connected multi-chamber device | |
| US20020031836A1 (en) | Fluidics system | |
| KR102375602B1 (en) | Modular micro-fluidic chip and micro-fluidic flow system having thereof | |
| JP2020525802A5 (en) | ||
| US20160116381A1 (en) | Swab port for microfluidic devices | |
| CN113231112A (en) | Microfluidic chip reagent storage device and application method thereof | |
| CN118165805B (en) | Multi-drive source kit, amplification detection equipment and amplification detection method | |
| CN112058324B (en) | Micro-fluidic chip | |
| CN111944672B (en) | Chip structure for molecular detection and detection method | |
| ES2632863B1 (en) | System of loading and unloading of air under controlled pressure | |
| CN112934277A (en) | Rapid low-consumption sample filling method for microfluidic chip | |
| US20230256429A1 (en) | Modular fluidic chip and fluidic flow system comprising same | |
| JPWO2017208772A1 (en) | Liquid injection attachment | |
| CN114308149B (en) | Chip sealing device and self-sealing modularized chip equipment | |
| Reichen et al. | Characterisation of an adhesive-free packaging system for polymeric microfluidic biochemical devices and reactors | |
| US11471883B2 (en) | Fluid chip, fluid device, and method for manufacturing same | |
| JP2019181427A (en) | adapter | |
| Etxeberria et al. | Critical Study on the Tube-to-Chip Luer Slip Connectors | |
| US20190328298A1 (en) | Emulsion production member, and sample collection tube and blood collection tube each equipped with same | |
| CN220126240U (en) | Chip bottom shell of micro-fluidic chip and micro-fluidic chip | |
| US20240342710A1 (en) | Device, in Particular Microfluidic Cartridge, and Method, Comprising a Removal Chamber and a Removable Cover | |
| CN221117405U (en) | Chip sequencing system for rapid sample injection | |
| CN218811631U (en) | Extrusion type micro-fluidic chip | |
| CN117384750B (en) | Fully integrated digital nucleic acid analysis cartridge | |
| CN114410448B (en) | Microfluidic chip for detecting multi-target nucleic acid and detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |