CN113230282A - Microecological bacteriostatic liquid and preparation method thereof - Google Patents
Microecological bacteriostatic liquid and preparation method thereof Download PDFInfo
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- CN113230282A CN113230282A CN202110589786.5A CN202110589786A CN113230282A CN 113230282 A CN113230282 A CN 113230282A CN 202110589786 A CN202110589786 A CN 202110589786A CN 113230282 A CN113230282 A CN 113230282A
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- 239000007788 liquid Substances 0.000 title claims abstract description 25
- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims abstract description 18
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 16
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 16
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 16
- 239000002207 metabolite Substances 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 9
- 235000013024 sodium fluoride Nutrition 0.000 claims abstract description 9
- 239000011775 sodium fluoride Substances 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 239000003755 preservative agent Substances 0.000 claims abstract description 7
- 230000002335 preservative effect Effects 0.000 claims abstract description 7
- 241000186660 Lactobacillus Species 0.000 claims abstract description 6
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 6
- 239000003906 humectant Substances 0.000 claims abstract description 5
- 238000009630 liquid culture Methods 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 13
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical group [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 239000004302 potassium sorbate Substances 0.000 claims description 5
- 235000010241 potassium sorbate Nutrition 0.000 claims description 5
- 229940069338 potassium sorbate Drugs 0.000 claims description 5
- 239000000600 sorbitol Substances 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 238000010899 nucleation Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 3
- 239000004299 sodium benzoate Substances 0.000 claims description 3
- 235000010234 sodium benzoate Nutrition 0.000 claims description 3
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical group OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 239000006041 probiotic Substances 0.000 claims 3
- 230000000529 probiotic effect Effects 0.000 claims 3
- 235000018291 probiotics Nutrition 0.000 claims 3
- 239000000022 bacteriostatic agent Substances 0.000 claims 2
- 238000001471 micro-filtration Methods 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 208000002925 dental caries Diseases 0.000 abstract description 8
- 206010059866 Drug resistance Diseases 0.000 abstract description 2
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 241000194019 Streptococcus mutans Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000012982 microporous membrane Substances 0.000 description 2
- QIJRTFXNRTXDIP-UHFFFAOYSA-N (1-carboxy-2-sulfanylethyl)azanium;chloride;hydrate Chemical compound O.Cl.SCC(N)C(O)=O QIJRTFXNRTXDIP-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 201000004328 Pulpitis Diseases 0.000 description 1
- 206010037464 Pulpitis dental Diseases 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229940040526 anhydrous sodium acetate Drugs 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 229960001305 cysteine hydrochloride Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 208000004480 periapical periodontitis Diseases 0.000 description 1
- 208000028169 periodontal disease Diseases 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 208000004371 toothache Diseases 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/20—Halogens; Compounds thereof
- A61K8/21—Fluorides; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention discloses a micro-ecological antibacterial liquid and a preparation method thereof, aiming at solving the technical problem of difficulty in preventing and treating children dental caries. The micro-ecological bacteriostatic solution consists of the following raw materials in percentage by weight: 20-30% of humectant, 1-5% of preservative, 0.02-0.1% of sodium fluoride and the balance of lactobacillus acidophilus filtrate containing fermentation metabolites. The microecological antibacterial liquid provided by the invention effectively avoids the problems of abuse of antibiotics and bacterial drug resistance of children, and the antibacterial gargle prepared from metabolites of lactobacillus is simple, convenient, safe and efficient to prevent and treat decayed teeth.
Description
Technical Field
The invention relates to the technical field of food, in particular to a micro-ecological antibacterial liquid and a preparation method thereof.
Background
Dental caries is also called tooth decay, is mainly a dental hard tissue destruction disease caused by factors such as bacterial infection and the like, is one of the most common diseases of preschool children, has the clinical symptoms of toothache, not only influences the chewing function of the children, but also hinders the growth of permanent teeth of the children. If the dental caries is not treated timely and effectively, complications such as pulpitis, periapical periodontitis and jacobsite inflammation can be caused, so that the physical and mental health of children is seriously affected, and attention is paid to the tooth health of the children at the period.
Dental caries is a disease caused by multifactorial factors, and the occurrence reason is to consider tooth constituent elements, form, position, saliva constituent, pH value, secretion amount, viscosity, antibacterial factor, carbohydrate content in diet, fluoride, vitamin and physical properties thereof, so that the number of bacteria in the oral cavity is reduced, and the incidence rate of dental caries and periodontal disease caused by bacteria can be reduced.
Disclosure of Invention
The invention aims to solve the technical problem of providing a micro-ecological antibacterial liquid and a preparation method thereof, so as to solve the technical problem of difficulty in preventing and treating children dental caries.
In order to solve the technical problems, the invention adopts the following technical scheme:
provides a micro-ecological bacteriostatic liquid which is prepared from the following raw materials in percentage by weight:
20-30% of a humectant, 1-5% of a preservative, 0.02-0.1% of sodium fluoride and the balance of a filtrate containing fermentation metabolites of lactobacillus acidophilus.
Preferably, the humectant is sorbitol or propylene glycol.
Preferably, the preservative is potassium sorbate or sodium benzoate.
Preferably, the method for preparing the lactobacillus acidophilus filtrate containing fermentation metabolites comprises the following steps:
(1) taking acidophilic lactobacillus suspension to transfer to an MRS agar culture medium plate, and culturing overnight at 37 ℃;
(2) inoculating the single colony subjected to overnight culture into an MRS liquid culture medium, and culturing for 2-3 days at 37 ℃;
(3) taking a liquid culture solution, inoculating the liquid culture solution into a seeding tank according to the inoculation amount of 3-5%, and culturing for 36-48 h at 37 ℃;
(4) inoculating the bacterial liquid cultured in the seed tank into a fermentation tank by an inoculation amount of 1-2%, and fermenting until the concentration of the bacterial reaches 106～108/ml；
(5) Centrifuging the fermentation liquor, collecting supernatant, and separating and filtering out viable bacteria.
Further, in the step (5), the filtering is to pass the fermentation liquor through a microporous membrane filtration device by using a membrane separation technology.
In addition, the preparation method of the micro-ecological bacteriostatic solution comprises the following steps:
(1) taking acidophilic lactobacillus suspension to transfer to an MRS agar culture medium plate, and culturing overnight at 37 ℃;
(2) inoculating the single colony subjected to overnight culture into an MRS liquid culture medium, and culturing for 2-3 days at 37 ℃;
(3) taking a liquid culture solution, inoculating the liquid culture solution into a seeding tank according to the inoculation amount of 3-5%, and culturing for 36-48 h at 37 ℃;
(4) inoculating the bacterial liquid cultured in the seed tank into a fermentation tank by an inoculation amount of 1-2%, and fermenting until the concentration of the bacterial reaches 106～108/ml；
(5) Centrifuging the fermentation liquor, collecting supernatant, and separating and filtering out viable bacteria to obtain lactobacillus acidophilus filtrate containing fermentation metabolites;
(6) and (3) mixing 20-30% of a moisturizing agent, 1-5% of a preservative, 0.02-0.1% of sodium fluoride and the balance of a filtrate containing fermentation metabolites of lactobacillus acidophilus, and thus obtaining the lactobacillus acidophilus beverage.
Compared with the prior art, the invention has the beneficial technical effects that:
the microecological antibacterial liquid provided by the invention effectively avoids the problems of abuse of antibiotics and bacterial drug resistance of children, and the antibacterial gargle prepared from metabolites of lactobacillus is simple, convenient, safe and efficient to prevent and treat decayed teeth.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but are not intended to limit the scope of the present invention in any way.
The instruments and devices referred to in the following examples are conventional instruments and devices unless otherwise specified; the reagents and raw materials are commercially available conventional reagents and culture media unless otherwise specified.
Example 1: culture of Lactobacillus acidophilus NCMF
(1) The Lactobacillus acidophilus NCMF strain was activated, and about 0.2mL of the suspension was transferred onto an MRS agar medium plate and cultured overnight at 37 ℃.
The MRS culture medium comprises the following components in parts by weight: peptone 1%, yeast extract powder 0.5%, beef extract 1%, glucose 2%, anhydrous sodium acetate 0.3%, diammonium citrate 0.2%, tween 800.1%, dipotassium hydrogen phosphate 0.2%, MgSO4 & 7H2O 0.02%，MnSO4·4H20.005% of O and 0.05% of cysteine hydrochloride, adjusting the pH value of the culture medium to 6.8, and sterilizing for 20min at 121 ℃.
(2) Liquid culture
Inoculating overnight cultured Lactobacillus acidophilus NCMF colony into a triangular flask containing MRS liquid culture medium, and standing in an incubator at 37 deg.C. Culturing for 2-3 days until the strain grows slowly and uniformly in the liquid culture medium and has obvious layering phenomenon.
(3) Expanding culture
Inoculating 5% of inoculum size into a sterilized seed tank under aseptic conditions of liquid culture, culturing at 37 ℃ for 48h, conveying the seed tank to a fermentation tank, fermenting for 48-72 h until the thallus concentration reaches 108/ml。
(4) Isolation of the fermentation broth
Centrifuging at 7000r/min to collect the fermentation liquid, separating by membrane separation technology, applying pressure under clean production environment to make the fermentation liquid pass through microporous membrane filtration equipment, and filtering out viable bacteria to obtain filtrate containing fermentation metabolite.
Example 2: microecological bacteriostatic liquid
The micro-ecological bacteriostatic liquid consists of the following raw materials in parts by weight:
20 parts of sorbitol, 1 part of potassium sorbate, 0.02 part of sodium fluoride and the balance of the filtrate containing the fermentation metabolites in example 1 are taken.
The surfactant in the fermentation liquor of the lactobacillus acidophilus NCMF can inhibit the synthesis of water-soluble and water-insoluble glucan of the streptococcus mutans of the dental caries pathogenic bacteria, so as to inhibit the formation of a streptococcus mutans biomembrane; bacteriocin in the fermentation broth of lactobacillus acidophilus NCMF can significantly inhibit the growth of streptococcus mutans.
Example 3: microecological bacteriostatic liquid
The micro-ecological bacteriostatic liquid consists of the following raw materials in parts by weight:
taking 25 parts of sorbitol, 2 parts of potassium sorbate, 0.05 part of sodium fluoride and the balance of the filtrate containing the fermentation metabolites in the example 1.
Example 4: microecological bacteriostatic liquid
The micro-ecological bacteriostatic liquid consists of the following raw materials in parts by weight:
28 parts of propylene glycol, 3 parts of sodium benzoate, 0.04 part of sodium fluoride and the balance of the filtrate containing the fermentation metabolites in the example 1 are taken.
Example 5: microecological bacteriostatic liquid
The micro-ecological bacteriostatic liquid consists of the following raw materials in parts by weight:
30 parts of sorbitol, 5 parts of potassium sorbate and 0.1 part of sodium fluoride are taken, and the balance is the filtrate containing the fermentation metabolites in the example 1.
Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes in the specific parameters of the embodiments may be made without departing from the spirit of the present invention, and a plurality of specific embodiments are formed, which are common variations of the present invention, and will not be described in detail herein.
Claims (6)
1. The microbial antibacterial liquid is characterized by comprising the following raw materials in percentage by weight:
20-30% of a humectant, 1-5% of a preservative, 0.02-0.1% of sodium fluoride and the balance of a filtrate containing fermentation metabolites of lactobacillus acidophilus.
2. The probiotic bacteriostatic solution according to claim 1, wherein the humectant is sorbitol or propylene glycol.
3. The probiotic bacteriostatic solution according to claim 1, wherein the preservative is potassium sorbate or sodium benzoate.
4. The probiotic bacteriostatic agent according to claim 1, wherein the lactobacillus acidophilus filtrate containing fermentation metabolites is prepared by the following steps:
(1) taking acidophilic lactobacillus suspension to transfer to an MRS agar culture medium plate, and culturing overnight at 37 ℃;
(2) inoculating the single colony subjected to overnight culture into an MRS liquid culture medium, and culturing for 2-3 days at 37 ℃;
(3) taking a liquid culture solution, inoculating the liquid culture solution into a seeding tank according to the inoculation amount of 3-5%, and culturing for 36-48 h at 37 ℃;
(4) inoculating the bacterial liquid cultured in the seed tank into a fermentation tank by an inoculation amount of 1-2%, and fermenting until the concentration of the bacterial reaches 106～108/ml；
(5) Centrifuging the fermentation liquor, collecting supernatant, and separating and filtering out viable bacteria.
5. The bacteriostatic agent according to claim 4, wherein in the step (5), the filtration is performed by passing the fermentation liquid through a microfiltration membrane filtration device by using a membrane separation technique.
6. The preparation method of the microecological bacteriostatic liquid according to claim 1, which is characterized by comprising the following steps:
(1) taking acidophilic lactobacillus suspension to transfer to an MRS agar culture medium plate, and culturing overnight at 37 ℃;
(2) inoculating the single colony subjected to overnight culture into an MRS liquid culture medium, and culturing for 2-3 days at 37 ℃;
(3) taking a liquid culture solution, inoculating the liquid culture solution into a seeding tank according to the inoculation amount of 3-5%, and culturing for 36-48 h at 37 ℃;
(4) inoculating the bacterial liquid cultured in the seed tank into a fermentation tank by an inoculation amount of 1-2%, and fermenting until the concentration of the bacterial reaches 106～108/ml；
(5) Centrifuging the fermentation liquor, collecting supernatant, and separating and filtering out viable bacteria to obtain lactobacillus acidophilus filtrate containing fermentation metabolites;
(6) and (3) mixing 20-30% of a moisturizing agent, 1-5% of a preservative, 0.02-0.1% of sodium fluoride and the balance of a filtrate containing fermentation metabolites of lactobacillus acidophilus, and thus obtaining the lactobacillus acidophilus beverage.
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| CN202110589786.5A CN113230282A (en) | 2021-05-28 | 2021-05-28 | Microecological bacteriostatic liquid and preparation method thereof |
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| CN107753930A (en) * | 2017-11-16 | 2018-03-06 | 江苏大学 | The preparation and its application of nisin, Cetylpyridinium Chloride and sodium fluoride mouthwash |
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