CN113230263B

CN113230263B - Phospholipid with high PC content and low acid value as well as preparation method and application thereof

Info

Publication number: CN113230263B
Application number: CN202110531430.6A
Authority: CN
Inventors: 冯玉梅; 郭静
Original assignee: Hebei Meiyesiweisheng Technology Co ltd
Current assignee: Hebei Meiyesiweisheng Technology Co ltd
Priority date: 2021-05-14
Filing date: 2021-05-14
Publication date: 2023-06-30
Anticipated expiration: 2041-05-14
Also published as: CN113230263A

Abstract

The invention discloses a high-PC content low-acid-value phospholipid, a preparation method and application thereof, wherein the high-PC content low-acid-value phospholipid comprises the following components in parts by weight: the soybean phospholipid PC-60 or sunflower seed phospholipid PC-60-65 parts, diglyceride oil 35-42 parts and vitamin E0.3-0.6 parts, wherein the PC content of the product prepared by the invention is 35% -38% of high PC content phospholipid on the premise of keeping the total phospholipid content of 55% -65%; meanwhile, the viscosity of the amber transparent liquid is less than or equal to 10000cp, and the phospholipid acid value with high PC content is less than or equal to 15mgKOH/g; the soybean phospholipid soft capsule has the characteristics of safety, health, definite functional factors, high content and good stability, and in addition, the preparation method of the soybean phospholipid soft capsule content is simple and fundamentally different from the existing commercially-available similar formula.

Description

Phospholipid with high PC content and low acid value as well as preparation method and application thereof

Technical Field

The invention belongs to the technical field of biochemistry, and relates to a phospholipid with high PC content and low acid value, a preparation method and application thereof.

Background

The technology for refining and separating phospholipids at home and abroad is rapidly developed, the phospholipid products are widely and deeply applied to the industries of medicines and health products, the effect of the phospholipids in the auxiliary treatment of various diseases has been proved by a large number of facts, and particularly, phosphatidylcholine is recognized as a main component playing an important physiological function, namely a functional factor, can provide choline components for human bodies, and has good curative effects on preventing and treating chronic diseases caused by fat metabolism disorder. People can treat chronic diseases such as hyperlipidemia, fatty liver, obesity and the like by orally taking a certain dose of phospholipid preparation and matching with other medicines. At present, the phospholipid health food takes the capsule as the most common, the content ingredients generally take soybean phospholipid and soybean oil as main raw materials, the capsule is in an amber sticky state, and the marking quality indexes are as follows: the total content of the phosphatide is 55% -60%, wherein the content of the lecithin (PC) is about 15%, and the diluent is soybean oil. Thus, such soyabean phospholipid capsule products suffer from four disadvantages: firstly, the intake of grease such as soybean oil in the product can increase the grease load of a human body, and the food is useless; secondly, the lecithin (PC) content in the product is low and generally not more than 15%, so that the product cannot have a good health care function; thirdly, because the phospholipid contains a large amount of unsaturated fatty acid, the acid value of the commercially available liquid phospholipid is 29mgKOH/g or more, generally about 31%, and the acid value is very high, so that the acid value is an important index for evaluating health-care food, regardless of foreign products or domestic products; fourth, when the PC content is increased from 12% to higher when the total phospholipid content is 62%, the viscosity tends to rise sharply or exceed 20000cp, the soft capsule is difficult or even impossible to fill, and the increase in viscosity tends to result in pill leakage.

Diglyceride oil (DG) is a structural lipid in which one fatty acid in Triglyceride (TAG) is replaced by hydroxyl, is a trace component of natural vegetable oil and fat, is a food component of world-recognized safety (GRAS), and is a new resource food approved by China. Clinical data show that the diglyceride edible oil has excellent improving effect on hyperlipidemia, obesity and the like. The future diglyceride edible oil is a necessary trend to replace part of triglyceride edible oil, and accords with objective historical development rules. It was found that diglycerides can enhance oxidation of fatty acids in the liver and reduce fatty acid synthesis. Diglycerides can inhibit fat accumulation by accelerating the beta-oxidation rate of fatty acids. The beta-oxidation process refers to the oxidation of fatty acids to eventually form carbon dioxide and water, and eventually form energy, without participating in the re-synthesis of triglycerides. The effects of diglyceride oil on increasing energy expenditure, fat oxidation and breath quotient are more pronounced in overweight people than in healthy people. The diglyceride is used to replace or partially replace common oil in food, so that appetite is not affected, weight gain is inhibited, and DG can be used to produce functional food with weight reducing effect, such as low calorie margarine, candy, chocolate, baked product, butter spread, mayonnaise, ice cream, etc. The diglyceride can reduce serum triglyceride of human and mice, and can be used for preventing and treating hyperlipidemia and cardiovascular and cerebrovascular diseases closely related to hyperlipidemia, such as arteriosclerosis, coronary heart disease, apoplexy, cerebral thrombosis, etc. DG is a trace component of natural vegetable oils and endogenous intermediate products of in vivo fat metabolism, and is a well-recognized and safe (GRAS) food ingredient. Scientific researches show that dietary DG has the effects of reducing visceral fat, inhibiting weight gain, reducing blood fat and the like, so that the dietary DG is widely paid attention to.

Vitamin E has various biological activities and has prevention and treatment effects on some diseases, is a strong antioxidant, and can protect the stability of cell membranes by interrupting chain reaction of free radicals in biological aspects, prevent lipofuscin from forming on the membranes and delay the aging of organisms; can regulate the metabolic activity of organisms by maintaining the stability of genetic materials and preventing the structural variation of chromosomes, also plays a role in delaying aging, and simultaneously VE is a good antioxidant for grease and prevents unsaturated fatty acids in the grease from being oxidized.

CN201410447157.9 discloses a soybean phosphatide soft capsule and its preparation method, the said content of the invention includes fish oil, soybean phosphatide, vitamin E and white beeswax, the components cooperate with each other, the effective components of soybean phosphatide soft capsule in phosphatidylcholine, EPA, DHA are improved. Because the technology for removing saturated fatty acid and cholesterol from the fish oil at present has some problems, the purity of the fish oil is often low, the low-purity fish oil contains excessive saturated fatty acid and cholesterol, and the long-term intake of the substances can lead to abnormal blood lipid, atherosclerosis and fish oil, which is not beneficial to the health of people suffering from cardiovascular diseases after long-term intake.

CN201310528936 discloses a six-dimensional phospholipid soft capsule and application thereof, the bulk pharmaceutical chemicals of the six-dimensional phospholipid soft capsule of the invention are: the raw material medicines of the six-dimensional phospholipid soft capsule are prepared by taking the soybean phospholipid powder and the soybean oil as raw materials instead of liquid phospholipid in the prior art, and the obtained six-dimensional phospholipid soft capsule is more homogeneous, does not delaminate and has the obvious effect of good long-term stability. The PC content in the soybean lecithin powder is generally about 22%, and if the soybean lecithin powder is prepared by mixing soybean oil with about 60% of the soybean oil, the PC content in the content is only about 14%.

CN201510411256.6 discloses a soybean lecithin soft capsule with hypolipidemic effect and its preparation method, the soybean lecithin soft capsule is mainly prepared by soybean lecithin, gingko extract, propolis, garlicin, vitamin E and pumpkin seed oil through a compound formulation, the preparation method is that 30-60% of soybean lecithin is compatible with other components, and the effects of ginkgo leaf extract, propolis, pumpkin seed oil and vitamin E are assisted, the compound synergistic effect is remarkable, the compound synergistic effect is important for reducing blood fat, keeping heart and brain circulation smooth and human health, 30-60% of soybean lecithin is 30-60% of total phospholipid content, and PC content is not high.

CN201610641595.8 discloses a soybean lecithin soft capsule and its preparation method, the soybean lecithin soft capsule of the invention comprises 30-60 parts of soybean lecithin, 30-60 parts of linseed oil, 0.1-0.5 part of VE, 2-8 parts of ethanol; the linseed oil with health-care function is used for replacing soybean oil, meanwhile, the addition of gelatin is canceled, and VE with health-care functions of resisting oxidation and resisting aging is added into the product, so that on one hand, the oxidation of soybean lecithin can be prevented, and the stability of the product can be enhanced. The total content of soybean phospholipids in the formula of the invention is 30-60 parts, and the PC content is not enhanced.

CN200810105088.8 discloses a hypolipidemic soft capsule health-care food and its preparation method, the patent adds notoginseng extract, ginkgo leaf extract into vegetable oil to dissolve, then adds DHA, EPA, soybean lecithin, beeswax and ethylcellulose to make products, the invention has advanced technology, the products have hypolipidemic health-care function, the adopted extraction method can extract notoginseng more effectively, ginkgo leaf effective component, remove coarse and store essence, greatly reduce dosage and improve curative effect, because of the conventional extraction method, the invention also uses fish oil formula, low purity fish oil can cause dyslipidaemia after long-term intake, atherosclerosis fish oil, and long-term intake is unfavorable for cardiovascular disease crowd health.

CN106455662 a discloses a nutrient combination comprising lipophilic active ingredients, in the examples disclosed in this patent a number of nutrient substances are provided dispersed with soybean oil, coconut oil, phospholipid substances and are not applied to diglyceride oils, although the lipid substances of this patent comprise monoglyceride oils and diglyceride oils, but they act as solvents and are not functional ingredients.

Disclosure of Invention

The invention aims to: in order to solve the defects of the phospholipid soft capsules in the prior art, the technical problem to be solved by the invention is to provide the phospholipid with high PC content and low acid value, wherein the high PC content is between 35 and 38 percent; simultaneously, the viscosity of the amber transparent liquid is less than or equal to 10000cp; meanwhile, the acid value is less than or equal to 15mgKOH/g.

The invention aims to solve the technical problem of providing a preparation method of phospholipid with high PC content and low acid value.

The invention finally solves the technical problem of providing the application of the phospholipid with high PC content and low acid value in the preparation of the hypolipidemic drug.

The technical scheme is as follows: in order to solve the technical problems, the invention provides high-PC content low-acid-value phospholipid, which is prepared from the following components in parts by weight: soybean phosphatide PC-60 or sunflower phosphatide PC-60-65, diglyceride oil 35-42, and vitamin E0.3-0.6.

Wherein, the PC content of the phospholipid with high PC content and low acid value is 35-38%, and the total phospholipid content is 55-65%.

Wherein the viscosity of the phospholipid with high PC content and low acid value is less than or equal to 10000cp, and the acid value is less than or equal to 15mgKOH/g.

Wherein, the total phospholipid content of the soybean phospholipid PC-60 or sunflower seed phospholipid PC-60 is 95% -98%, and the PC content is 55% -60%.

The invention also discloses a preparation method of the phospholipid with high PC content and low acid value, which comprises the following steps: placing soybean phosphatide PC-60 or sunflower phosphatide PC-60 into a material mixing tank, adding diglyceride oil, soaking for 12-24 hours, heating to 50-60 ℃, stirring, grinding, homogenizing, adding vitamin E, uniformly mixing to form uniform and stable viscous material, and sieving to obtain the soybean phosphatide. Vitamin E in the formula of the invention is a natural antioxidant, natural soybean phospholipids are easy to oxidize and deteriorate, and after VE is added into the product, the stability of the product is enhanced.

The grinding process uses a shearing and emulsifying pump, and the plastic and adhesive PC blocks are crushed, mixed, dispersed and homogenized under the shearing force of 2900 rpm high-speed rotation.

The invention also discloses application of the phospholipid with high PC content and low acid value in preparing a medicament for preventing or treating hyperlipidemia.

The functional factor phosphatidylcholine plays an important physiological role, can provide choline components for human bodies, has the effects of reducing fat intake and reducing blood lipid level, and has good curative effects on preventing and treating hyperlipidemia, fatty liver and chronic diseases associated with fat metabolic disorder; the diglyceride oil for blocking the absorption of human triglyceride is used for replacing soybean oil or other grease in the formula, so that the grease load of common phospholipid soft capsule products on the bodies of people with hyperlipidemia is avoided, and the absorption way of triglyceride is blocked by the addition part of the diglyceride oil. Specifically, the diglyceride edible oil adopted in the formula of the invention is digested into the monoglyceride in the intestinal tract, the monoglyceride is hydrolyzed into fatty acid, fat is not accumulated in the human body, the fatty acid is directly metabolized into energy, and the metabolic process can also "fry out" superfluous fat, so that the food has the effects of inhibiting weight, reducing visceral fat and reducing blood sugar. The diglyceride oil and lecithin are compatible and synergistically combined to form a healthy and safe product with definite functional factors and good effects of preventing and treating hyperlipidemia and related cardiovascular and cerebrovascular diseases.

The beneficial effects are that: compared with the prior art, the invention has the following remarkable advantages: the product prepared by the invention particularly strengthens the content of lecithin functional factors (phosphatidylcholine) on the premise of keeping 55% -65% of the total phospholipid content, namely, soybean phospholipid PC-60 or sunflower seed phospholipid PC-60 is selected as a raw material, and the PC content in the formula is strengthened to 35% -38% by utilizing an emulsification technology, so that the marking quality index below the high PC content low acid value phospholipid of the invention is satisfied, namely, the high PC content phospholipid with 35% -38% of PC content; simultaneously, the viscosity of the amber transparent liquid is less than or equal to 10000cp; meanwhile, the phospholipid acid value with high PC content is less than or equal to 15mgKOH/g; the functional grease of the new resource food is used in the formula, the functionality of the product is increased, and the novel food has the combination of the application of the novel product and the novel technology and the novel health concept. The invention has the characteristics of safety, health, definite functional factors, high content and no layering, and has healthier health care or treatment effects for users, especially people with triglyceride metabolism problems such as hyperlipidemia, obesity and the like. In addition, the preparation method of the soybean lecithin soft capsule content is simple and is fundamentally different from the existing commercially-available similar formula.

Drawings

FIG. 1 is a diagram of a liquid chromatography test sample of example 1; the abscissa of the graph is retention time (min), the ordinate is response value (mV) of the component, the retention time is 5.826, the PC peak is the rest of the peaks of other components;

FIG. 2 is a diagram of a liquid chromatography test sample in example 2; the abscissa of the graph is retention time (min), the ordinate is response value (mV) of the component, the retention time is 6.208, the PC peak is the rest of the peaks of other components;

FIG. 3 is a diagram of a liquid chromatography test sample in example 3; the abscissa of the graph is retention time (min), the ordinate is response value (mV) of the component, the retention time is 6.732, the PC peak is the rest of the peaks of other components;

FIG. 4 is a diagram of a liquid chromatography test sample of comparative example 1; the abscissa of the graph is retention time (min), the ordinate is response value (mV) of the component, the retention time is 6.600, the PC peak is the rest of the peaks of other components;

FIG. 5 is a diagram of a liquid chromatography test sample of comparative example 2; the abscissa of the graph is retention time (min), the ordinate is response value (mV) of the component, the retention time is 6.711, the PC peak is the rest of the peaks of other components;

FIG. 6 is a diagram of a liquid chromatography test sample of comparative example 3; the graph has a retention time (min) on the abscissa, a response value (mV) of the component on the ordinate, a PC peak with a retention time of 6.188, and other peaks of other components.

Detailed Description

The technical scheme of the invention is further described below.

In the technical scheme of the invention, soybean lecithin PC-60 (batch No. 20210202) and sunflower lecithin PC-60 (batch No. 20210104) are used as products produced by the company (Hebei Meiss vitamin technology Co., ltd.), and other raw materials of the invention are available in the market. The invention firstly compares the related performances of lecithin capsule products at home and abroad with soybean lecithin PC-60 and sunflower lecithin PC-60. The specific results are shown in Table 1:

example 1

Adding 35 parts of diglyceride oil into a batching tank according to the weight part, then adding 65 parts of soybean lecithin PC-60 material, heating to 60 ℃ and preserving heat for soaking for 24 hours to soften and dissolve the plastic soybean lecithin PC-60 material, stirring for 60 minutes, adding 0.6 part of VE, adding a shearing emulsifying pump, rotating at 2900 r/min, homogenizing to amber transparent state, detecting acetone insoluble matter 59.5%, PC content 35.6%, acid value 12.1mgKOH/g, detecting sample viscosity 9200cp by a viscometer, peroxide value 2.1meq/kg, detecting sample PC spectrum by liquid phase chromatography according to figure 1 and table 2, wherein the peak of the retention time at 5.826 is PC peak, and the PC content of the sample is 35.6% according to calculation.

TABLE 2

Example 2

Adding 40 parts of diglyceride oil into a batching tank according to the weight part, then adding 60 parts of sunflower lecithin PC-60 material, heating to 60 ℃, preserving heat and soaking for 24 hours to soften and dissolve the plastic sunflower lecithin PC-60 material, stirring for 60 minutes, adding 0.6 part of VE, adding a shearing emulsifying pump, rotating at 2900 r/min, homogenizing to amber transparent state, detecting acetone insoluble matter 58.7%, PC content 36.7%, acid value 10.4mgKOH/g, detecting sample viscosity 8600CP by a viscometer, detecting sample PC spectrum according to figure 2 and table 3, keeping peak at 6.208 as PC peak, and calculating that the PC content of the sample is 36.7%.

TABLE 3 Table 3

Example 3

Adding 42 parts of diglyceride oil into a batching tank according to the weight part, then adding 58 parts of soybean lecithin PC-60 material, heating to 60 ℃, preserving heat and soaking for 24 hours to soften and dissolve the plastic soybean lecithin PC-60 material, stirring for 60 minutes, adding 0.6 part of VE, feeding into an emulsification pump, rotating at 2900 r/min, homogenizing to amber transparent state, detecting acetone insoluble substances 55.2%, PC content 35.2%, acid value 9.3mgKOH/g, and measuring sample viscosity 4100CP by a viscometer. The PC spectra of the liquid chromatography test samples are shown in FIG. 3 and Table 4, the peak at 6.732 retention time is PC peak, and the PC content of the sample is 35.2% according to calculation.

TABLE 4 Table 4

Comparative example 1

According to the weight portion, 42 portions of diglyceride oil are added into a material mixing tank according to the formula, and then 58 portions of American Jia Ji Linzhi product are added

SB, 100002719, SOY LECHITHIN, LOT # 0007600907), stirring for 60min, adding 0.6 part of VE, mixing uniformly until the mixture is amber transparent, detecting 36.1% of acetone insoluble substances, 7.8% of PC content, 17.4mgKOH/g of acid value, and measuring the sample viscosity of 820CP by a viscometer. From the above examination, it can be seen that the viscosity according to the formulation can meet the predetermined requirements, but the phospholipid content and PC content are well below the limit values of examples 1 to 3 of the present invention, and the acid value is also above the limit values. The PC spectrum of the liquid chromatography detection sample is shown in fig. 4 and table 5, the peak with the retention time of 6.600 is PC peak, according to calculation,the PC content of this sample was 7.8%.

TABLE 5

Comparative example 2

Adding 50 parts of diglyceride oil into a material mixing tank according to the formula, and then adding 50 parts of American Jia Ji Linzhi product [ ] according to the weight part

SB, 100002719, SOY LECHITHIN, LOT # 0007600907), stirring for 60min, adding 0.6 part of VE, stirring uniformly until the mixture is amber transparent, detecting 29.1% of acetone insoluble substances, 6.5% of PC content and 14.5mgKOH/g of acid value, and measuring 200CP of sample viscosity by a viscometer; when the acid number and viscosity can meet the limit requirements, the phospholipid content and PC content are well below the limit of the present invention. The PC spectra of the liquid chromatography test samples are shown in FIG. 5 and Table 6, the peak at 6.711 retention time is PC peak, and the PC content of the sample is 6.5% according to calculation.

TABLE 6

Comparative example 3

Adding 5 parts by weight of soybean lecithin PC-60 into a preparation tank according to a formula, and then adding 95 parts by weight of American Jia Ji Linzhi product [ ]

SB, 100002719, SOY LECHITHIN, LOT # 0007600907), stirring for 60min, adding 0.6 part of VE, stirring and homogenizing until the mixture is transparent, detecting acetone insoluble 61.65%, PC content 17.1%, acid value 29.3mgKOH/g, and measuring sample viscosity 9610CP by a viscometer; when the phospholipid content and viscosity can meet the limit requirements, the PC content is still well below the limit of the invention, and the acid value is also above the limit. PC (polycarbonate) spectrum of liquid chromatography detection sampleAs shown in fig. 6 and table 7, the peak at the retention time of 6.188 was a PC peak, and the PC content of the sample was 17.1% according to the calculation.

TABLE 7

As can be seen from the above performance tests of the specific products of examples 1-3 and comparative examples 1-3, the products of the present invention can maintain a high PC content of 35% -38% while the viscosity is 10000cp or less and the acid value is 15mgKOH/g or less, whereas the comparative examples 1-3 cannot satisfy the present invention at the same time in terms of PC content or acid value and viscosity.

Claims

1. The phospholipid with high PC content and low acid value is characterized by comprising the following components in parts by weight: soybean phosphatide PC-60 or sunflower phosphatide PC-6058-65, diglyceride oil 35-42, vitamin E0.3-0.6,

the PC content of the high PC content low acid value phosphatide is 35% -38%, the total phosphatide content is 55% -65%, the viscosity of the high PC content low acid value phosphatide is less than or equal to 10000cp, the acid value is less than or equal to 15mgKOH/g,

the total phospholipid content of the soybean phospholipid PC-60 or sunflower phospholipid PC-60 is 95-98 percent, the PC content is 55-60 percent,

the preparation method of the phospholipid with high PC content and low acid value comprises the following steps: placing soybean phosphatide PC-60 or sunflower phosphatide PC-60 into a material mixing tank, adding diglyceride oil, soaking for 12-24 hours, heating to 50-60 ℃, stirring, grinding and homogenizing, adding vitamin E, uniformly mixing to form uniform and stable viscous material, sieving, obtaining the soybean phosphatide PC-60 or sunflower phosphatide PC-60, wherein a shearing emulsifying pump is used in the grinding process,

the rotating speed of the shearing emulsifying pump is 2000-2900 rpm.

2. The use of a high PC content low acid number phospholipid as defined in claim 1 in the manufacture of a medicament for the prevention or treatment of hyperlipidemia.